FvH4_6g51610

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000159
Physical Location & Seq
Reverse (-)
38247878 .. 38252407
4530 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g51610.t2

Sequence Viewer

Length: 2655 bp
ATGGAGTTTGCCGCCATCATTGCTGAGAACGTCCTGGGAAGGCTAGCTTCCCATGCTTCCCAAGAGGTCTTTTTGGCATGGGGTGCCCAACTTGAGCTCACAAAGCTCAAGAAGACTTTATCCACCATCAATCTAGTGCTTCAGGATGCAGAGAAGAAGCAAGTGAAGAATCTCCTAATCACTCATTGGTTGGGAAAGCTCAAAGATGTTTGTTACGACGTCGATGATGTCCTTGACGAACTGGAGTTTCGAAAGTTGAGGAAGAAAGTGCTGACCAACAACCATGGGCGTGTCAAAGGACAGGTACGCCACTTTTTTTCGCGTTGGAGTCCAACAGTGTTTAACTTCAATATAGGACATAAGGTGAAAGAGATTAGAGAACGACTTGTTGAAATTGATAATGAAAAGAGACAATTTGCTCTGGTTGAGGTGCCTAAGATAGCTGAAGATCATAGTGTGCCCCAAGGGATGCAATACAATCAAAGAGAAACTGATTCCTTGGTAGAGGCTTCAGAAGTTATTGGGAGAGATGATGATAAAGAGCTAATTTTTAGTCATCTCTTACATAACACTGATATCTCTAATGAGGAGAATGTTTCTGTTATTTCAATCCTTGGGTTGGGGGGGTTGGGTAAGACAACGCTGGCTAAATTGGTGTACAATGATAGCGTGGTAGAGGAGAATTTTGAGGTGAAAATGTGGGTATGTGTCTCAGAAAACTTTGAAGTCAAATCATTAATTCTCAAGATTATCAATGCTGCAACTGATCAAAAATGTGAGGAGGAAAGTTTGGACAGTATGAAAAGAAGGTTGCAAGATACTTTGAGAGGTAGAAAGTTTTTGTTAGTGTTAGATGATGTTTGGGATACGGAGTCTATTGGAGTAACACTAGAAAAATGGTTGGGTTTAAAAAGTCTGTTATGTGTGGGAGCTAATGGAAGTAGAATCCTCGTAACAACACGAAATAGATCAGTTGCTTTACTCGTGAATCCTTCATACATGCATTCTTTGAAAGGTCTTCCCCACAAAGACTGCATGTCCTTGTTCATCAAAAGGGCATTGAAGAAAGGAGAGGAACAACGCTATCCACATTTGATAGAAATTGGAGAGGATATTGTGAACAAGTGTGGAGGAGTTCCTTTAGCTGTAGCAACTCTAGGGAGCATGCTCTATTTGAACAGAGAGCCAGACCGATGGTTGAGTGTGAGAGATGATGACATGTGGAGTATAGGAAATGACCATATTCTACCTGCACTCAAATTGAGCTATGATGCATTGCCACAACACTTGAAACCATGTTTTGCATTTTGTTCACTTTTCCCAAAGGATTTTGAATACTATAGTGAGTCACTAGTTGACCTGTGGGTAGCACAAGGCTACCTCAAAACATGTAAAAAAAATGAAGATCTTGGTAAAATGGGTATGGACTATATAAGACAGTTCTGCTCTAGATCATTGTTTCAAGTTGAAAACGATTTCAAAACCATTATGATGTTTAAAATGCATGATCTAGTTCATGATATAGCAATTTCGGTGGCACAAACAGAGTACTCCACAGTCAATTTTCGTCCCTCTAGTGCTTTTGAAATGGTTCGACATGTGTCAATATCCGACAAAGACATGCTTGGACAAGAAGCACAAGTGCCTGATTTCATATTCAAGTCAACCAAATTGCGTTCCTTTCTAATTCCATCTAGAGCTGGCAAGATGATGAATCAACGTTTTGTAAAGACATGCATCTCGAGATTCAAATATATGCGGGTGCTAGATCTCAGTGGGTCGCCCCTTGAGAAGCTACCAAGTTCCATTGGTAGTTTGTTTCATCTGAGATTTCTCGACTTGTCTTCCAATGATAAGATAAAAAGGCTTCCGAATTCCATCTGTAATTTACTGAATTTGGAGTCCTTGATCCTTCGTCAATGTACCGCACTTGAGGAGATACCCAAAGACATAGGGAACCTGATCAACCTCCGAAGCTTGGCGATAACTGCACAGCAGAAGTATTTACCAAAAGGAATTAGTCGCCTCACTTCACTCCAACTTTTACATTTTTTTGGATGTGTCAATCTGATGACTTTGGGGGAAGAGATCCAATTCCTCACTAACCTTCGTAGATTGGGGATTTCCAACTGTGAAAATTTGGAATCCTTGCCACCAAATATGAAACTCTTGACCGCTCTGCATAGTCTACATATAGATGAGTGTAAAAAGCTTGATTTGATGAAAACCAGAGAAGGCCCTCAAGGTCTTAGATCATTTATCATCTGCGTTTCAGATTTAGAGGTTTTGCCTCCCTGGCTTGAAGATTCTGCAAAAGTTTTGCAGAGCATAACAGTCGCGGGGTGTGATAATCTGACTGAACTTCCGGAACTGCAAAAATTTACATTCCTCGAGCAACTATTGATTGGAAGTTGTTCCAATTTGTTAGCTTTACCGCAAGGGTTACATTGCCTCACTGAGTTGAGAGAATTGGAAATTAATGATTGTCCCAAACTGAGCAAGAGCTGCAAAAGGCAATTAAAAGGTGGGGACTGGTCCAAGTTTGCACGGGAGGCAAAGATTACACTTGACTCTGATGTGGATACGGACGAAGAGGAAGACAAAGATGAAGGTGCTTTCGACGATCACAATGCTATCTCAAGTGATAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

885

Amino Acids

100.48

Weight (kDa)

6.12

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 94 8.4e-21 Rx N-terminal domain
NB-ARC PF00931 194 - 356 7.1e-32 NB-ARC domain
WHD_DRP PF23559 439 - 509 1.3e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 557 - 771 1.6e-28 Leucine-rich repeat region
LRR_RPS2 PF23247 602 - 736 7.6e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 222
Acc36I ACCTGC 1 cut(s) 1258
AccB1I GGYRCC 2 cut(s) 83, 430
AccBSI CCGCTC 1 cut(s) 2177
AccI GTMKAC 1 cut(s) 2188
AccII CGCG 2 cut(s) 322, 2339
AccIII TCCGGA 1 cut(s) 2365
AciI CCGC 6 cut(s) 12, 1759, 1926, 2175, 2339, 2435
AclI AACGTT 1 cut(s) 1720
AclWI GGATC 2 cut(s) 1903, 2083
AcsI RAATTY 5 cut(s) 682, 1873, 1894, 2137, 2378
AcuI CTGAAG 3 cut(s) 125, 465, 495
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 4 cut(s) 306, 659, 1550, 1924
AfiI CCNNNNNNNGG 2 cut(s) 619, 1978
AflIII ACRYGT 3 cut(s) 1218, 1388, 1597
AhdI GACNNNNNGTC 1 cut(s) 1036
AhlI ACTAGT 1 cut(s) 1351
AjnI CCWGG 2 cut(s) 33, 2294
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 2 cut(s) 403, 715
AlwI GGATC 2 cut(s) 1903, 2083
Ama87I CYCGRG 2 cut(s) 1741, 2390
Aor13HI TCCGGA 1 cut(s) 2365
AoxI GGCC 1 cut(s) 2236
ApeKI GCWGC 2 cut(s) 758, 2505
ApoI RAATTY 5 cut(s) 682, 1873, 1894, 2137, 2378
AseI ATTAAT 2 cut(s) 737, 2478
Asp700I GAANNNNTTC 2 cut(s) 1590, 2413
AspS9I GGNCC 2 cut(s) 2237, 2535
AsuHPI GGTGA 2 cut(s) 376, 703
AsuII TTCGAA 1 cut(s) 250
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 2 cut(s) 1741, 2390
AvaII GGWCC 1 cut(s) 2535
BaeGI GKGCMC 2 cut(s) 88, 462
BanI GGYRCC 2 cut(s) 83, 430
BanII GRGCYC 1 cut(s) 99
BauI CACGAG 1 cut(s) 983
BbsI GAAGAC 4 cut(s) 119, 1010, 1836, 2604
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 2 cut(s) 745, 2492
BccI CCATC 5 cut(s) 23, 134, 1188, 1699, 1886
BcgI CGANNNNNNTGC 4 cut(s) 2317, 2351, 2612, 2646
BciT130I CCWGG 2 cut(s) 35, 2296
BciVI GTATCC 2 cut(s) 859, 2575
BclI TGATCA 2 cut(s) 766, 1962
BcoDI GTCTC 2 cut(s) 403, 715
BcuI ACTAGT 1 cut(s) 1351
BfmI CTRYAG 2 cut(s) 1146, 1339
BfuAI ACCTGC 1 cut(s) 1258
BfuI GTATCC 2 cut(s) 859, 2575
BglI GCCNNNNNGGC 1 cut(s) 2296
BglII AGATCT 2 cut(s) 1405, 1768
BisI GCNGC 3 cut(s) 12, 759, 2506
BlsI GCNGC 3 cut(s) 13, 760, 2507
BmcAI AGTACT 1 cut(s) 1550
Bme1390I CCNGG 2 cut(s) 35, 2296
Bme18I GGWCC 1 cut(s) 2535
BmeRI GACNNNNNGTC 1 cut(s) 1036
BmeT110I CYCGRG 2 cut(s) 1741, 2390
BmgT120I GGNCC 2 cut(s) 2237, 2535
BmiI GGNNCC 3 cut(s) 85, 432, 1958
BmrFI CCNGG 2 cut(s) 35, 2296
BmsI GCATC 4 cut(s) 136, 459, 1261, 1746
BmtI GCTAGC 1 cut(s) 47
BoxI GACNNNNGTC 1 cut(s) 1600
BpiI GAAGAC 4 cut(s) 119, 1010, 1836, 2604
BpmI CTGGAG 1 cut(s) 263
Bpu14I TTCGAA 1 cut(s) 250
BpuEI CTTGAG 7 cut(s) 92, 113, 728, 1808, 1952, 2226, 2623
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 6 cut(s) 34, 283, 463, 498, 613, 2294
BsaWI WCCGGW 1 cut(s) 2365
Bsc4I CCNNNNNNNGG 2 cut(s) 619, 1978
Bse1I ACTGG 2 cut(s) 246, 2537
Bse3DI GCAATG 3 cut(s) 18, 1274, 2446
BseAI TCCGGA 1 cut(s) 2365
BseBI CCWGG 2 cut(s) 35, 2296
BseDI CCNNGG 6 cut(s) 34, 283, 463, 498, 613, 2294
BseGI GGATG 3 cut(s) 151, 474, 2063
BseLI CCNNNNNNNGG 2 cut(s) 619, 1978
BseMI GCAATG 3 cut(s) 18, 1274, 2446
BseMII CTCAG 6 cut(s) 15, 726, 1786, 1817, 2448, 2486
BseNI ACTGG 2 cut(s) 246, 2537
BseRI GAGGAG 5 cut(s) 602, 692, 794, 1146, 1949
BseSI GKGCMC 2 cut(s) 88, 462
BseXI GCAGC 2 cut(s) 745, 2492
BsgI GTGCAG 2 cut(s) 1236, 1974
Bsh1236I CGCG 2 cut(s) 322, 2339
BshFI GGCC 1 cut(s) 2238
BshNI GGYRCC 2 cut(s) 83, 430
BsiHKAI GWGCWC 1 cut(s) 99
BsiHKCI CYCGRG 2 cut(s) 1741, 2390
BsiSI CCGG 1 cut(s) 2366
BslFI GGGAC 3 cut(s) 1554, 2472, 2543
BslI CCNNNNNNNGG 2 cut(s) 619, 1978
BsmAI GTCTC 2 cut(s) 403, 715
BsmFI GGGAC 3 cut(s) 1554, 2472, 2543
BsmI GAATGC 1 cut(s) 1003
BsnI GGCC 1 cut(s) 2238
BsoBI CYCGRG 2 cut(s) 1741, 2390
Bsp119I TTCGAA 1 cut(s) 250
Bsp1286I GDGCHC 3 cut(s) 88, 99, 462
Bsp13I TCCGGA 1 cut(s) 2365
Bsp1407I TGTACA 1 cut(s) 657
Bsp19I CCATGG 1 cut(s) 283
BspACI CCGC 6 cut(s) 12, 1759, 1926, 2175, 2339, 2435
BspANI GGCC 1 cut(s) 2238
BspCNI CTCAG 6 cut(s) 16, 725, 1785, 1818, 2449, 2487
BspEI TCCGGA 1 cut(s) 2365
BspFNI CGCG 2 cut(s) 322, 2339
BspHI TCATGA 1 cut(s) 1516
BspLI GGNNCC 3 cut(s) 85, 432, 1958
BspMI ACCTGC 1 cut(s) 1258
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 2 cut(s) 1903, 2083
BspT104I TTCGAA 1 cut(s) 250
BspT107I GGYRCC 2 cut(s) 83, 430
BsrBI CCGCTC 1 cut(s) 2177
BsrDI GCAATG 3 cut(s) 18, 1274, 2446
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 2 cut(s) 246, 2537
BssECI CCNNGG 6 cut(s) 34, 283, 463, 498, 613, 2294
BssNI GRCGYC 1 cut(s) 219
BssSI CACGAG 1 cut(s) 983
BssT1I CCWWGG 4 cut(s) 283, 463, 498, 613
Bst2BI CACGAG 1 cut(s) 983
Bst2UI CCWGG 2 cut(s) 35, 2296
Bst4CI ACNGT 6 cut(s) 337, 797, 1440, 1558, 2132, 2335
Bst6I CTCTTC 2 cut(s) 2079, 2586
BstACI GRCGYC 1 cut(s) 219
BstAPI GCANNNNNTGC 2 cut(s) 83, 2505
BstAUI TGTACA 1 cut(s) 657
BstBI TTCGAA 1 cut(s) 250
BstC8I GCNNGC 4 cut(s) 45, 645, 1166, 1702
BstDEI CTNAG 8 cut(s) 24, 435, 712, 1772, 1826, 2249, 2457, 2495
BstDSI CCRYGG 1 cut(s) 283
BstF5I GGATG 3 cut(s) 151, 474, 2063
BstFNI CGCG 2 cut(s) 322, 2339
BstMAI GTCTC 2 cut(s) 403, 715
BstMWI GCNNNNNNNGC 8 cut(s) 20, 53, 83, 103, 1988, 2296, 2505, 2552
BstNI CCWGG 2 cut(s) 35, 2296
BstNSI RCATGY 8 cut(s) 1003, 1039, 1168, 1222, 1392, 1601, 1624, 1737
BstPAI GACNNNNGTC 1 cut(s) 1600
BstSCI CCNGG 2 cut(s) 33, 2294
BstSFI CTRYAG 2 cut(s) 1146, 1339
BstSLI GKGCMC 2 cut(s) 88, 462
BstUI CGCG 2 cut(s) 322, 2339
BstV1I GCAGC 2 cut(s) 745, 2492
BstV2I GAAGAC 4 cut(s) 119, 1010, 1836, 2604
BstX2I RGATCY 3 cut(s) 1405, 1768, 2088
BstXI CCANNNNNNTGG 1 cut(s) 1194
BstYI RGATCY 3 cut(s) 1405, 1768, 2088
BsuI GTATCC 2 cut(s) 859, 2575
BsuRI GGCC 1 cut(s) 2238
BtgI CCRYGG 1 cut(s) 283
BtsCI GGATG 3 cut(s) 151, 474, 2063
BtsIMutI CAGTG 4 cut(s) 342, 570, 1780, 2454
BveI ACCTGC 1 cut(s) 1258
Cac8I GCNNGC 4 cut(s) 45, 645, 1166, 1702
CciI TCATGA 1 cut(s) 1516
Cfr13I GGNCC 2 cut(s) 2237, 2535
Csp6I GTAC 4 cut(s) 305, 658, 1549, 1923
CspCI CAANNNNNGTGG 4 cut(s) 1269, 1304, 1515, 1550
CviQI GTAC 4 cut(s) 305, 658, 1549, 1923
DdeI CTNAG 8 cut(s) 24, 435, 712, 1772, 1826, 2249, 2457, 2495
DraI TTTAAA 2 cut(s) 909, 1498
DriI GACNNNNNGTC 1 cut(s) 1036
Eam1104I CTCTTC 2 cut(s) 2079, 2586
Eam1105I GACNNNNNGTC 1 cut(s) 1036
EarI CTCTTC 2 cut(s) 2079, 2586
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 4 cut(s) 283, 463, 498, 613
Eco24I GRGCYC 1 cut(s) 99
Eco32I GATATC 1 cut(s) 577
Eco47I GGWCC 1 cut(s) 2535
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 3 cut(s) 125, 465, 495
Eco88I CYCGRG 2 cut(s) 1741, 2390
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 2237
EcoRI GAATTC 1 cut(s) 1873
EcoRII CCWGG 2 cut(s) 33, 2294
EcoRV GATATC 1 cut(s) 577
EcoT14I CCWWGG 4 cut(s) 283, 463, 498, 613
EcoT22I ATGCAT 4 cut(s) 1005, 1276, 1506, 1739
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 4 cut(s) 283, 463, 498, 613
FalI AAGNNNNNCTT 4 cut(s) 31, 63, 1608, 1640
FaqI GGGAC 3 cut(s) 1554, 2472, 2543
FauI CCCGC 2 cut(s) 1752, 2332
FbaI TGATCA 2 cut(s) 766, 1962
FblI GTMKAC 1 cut(s) 2188
Fnu4HI GCNGC 3 cut(s) 12, 759, 2506
FokI GGATG 3 cut(s) 158, 481, 2070
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 3 cut(s) 12, 759, 2506
GluI GCNGC 3 cut(s) 12, 759, 2506
GsuI CTGGAG 1 cut(s) 263
HaeIII GGCC 1 cut(s) 2238
HapII CCGG 1 cut(s) 2366
Hin1I GRCGYC 1 cut(s) 219
HincII GTYRAC 2 cut(s) 1357, 1665
HindII GTYRAC 2 cut(s) 1357, 1665
HindIII AAGCTT 2 cut(s) 1975, 2210
HpaII CCGG 1 cut(s) 2366
HphI GGTGA 2 cut(s) 376, 703
Hpy166II GTNNAC 6 cut(s) 658, 1120, 1313, 1357, 1665, 2189
Hpy8I GTNNAC 6 cut(s) 658, 1120, 1313, 1357, 1665, 2189
Hpy99I CGWCG 3 cut(s) 221, 224, 2624
HpyAV CCTTC 7 cut(s) 33, 801, 1002, 1922, 2117, 2228, 2603
HpyCH4III ACNGT 6 cut(s) 337, 797, 1440, 1558, 2132, 2335
HpyCH4IV ACGT 3 cut(s) 30, 219, 1720
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 53, 83, 103, 1988, 2296, 2505, 2552
HpyF3I CTNAG 8 cut(s) 24, 435, 712, 1772, 1826, 2249, 2457, 2495
HpySE526I ACGT 3 cut(s) 30, 219, 1720
Hsp92I GRCGYC 1 cut(s) 219
Kpn2I TCCGGA 1 cut(s) 2365
Ksp22I TGATCA 2 cut(s) 766, 1962
LmnI GCTCC 2 cut(s) 929, 1161
Lsp1109I GCAGC 2 cut(s) 745, 2492
LweI GCATC 4 cut(s) 136, 459, 1261, 1746
MaeII ACGT 3 cut(s) 30, 219, 1720
MaeIII GTNAC 5 cut(s) 212, 883, 952, 1347, 2442
MbiI CCGCTC 1 cut(s) 2177
MflI RGATCY 3 cut(s) 1405, 1768, 2088
MhlI GDGCHC 3 cut(s) 88, 99, 462
MlyI GAGTC 5 cut(s) 337, 881, 1355, 1910, 2564
MmeI TCCRAC 5 cut(s) 305, 356, 1635, 2062, 2151
Mph1103I ATGCAT 4 cut(s) 1005, 1276, 1506, 1739
MroI TCCGGA 1 cut(s) 2365
MroXI GAANNNNTTC 2 cut(s) 1590, 2413
MseI TTAA 6 cut(s) 342, 737, 908, 1497, 2478, 2519
MslI CAYNNNNRTG 3 cut(s) 288, 1490, 2196
MspI CCGG 1 cut(s) 2366
MspR9I CCNGG 2 cut(s) 35, 2296
Mva1269I GAATGC 1 cut(s) 1003
MvaI CCWGG 2 cut(s) 35, 2296
MvnI CGCG 2 cut(s) 322, 2339
MwoI GCNNNNNNNGC 8 cut(s) 20, 53, 83, 103, 1988, 2296, 2505, 2552
NcoI CCATGG 1 cut(s) 283
NheI GCTAGC 1 cut(s) 43
NlaIV GGNNCC 3 cut(s) 85, 432, 1958
NmuCI GTSAC 1 cut(s) 1347
NsiI ATGCAT 4 cut(s) 1005, 1276, 1506, 1739
NspI RCATGY 8 cut(s) 1003, 1039, 1168, 1222, 1392, 1601, 1624, 1737
NspV TTCGAA 1 cut(s) 250
PaeI GCATGC 1 cut(s) 1168
PaeR7I CTCGAG 2 cut(s) 1741, 2390
PagI TCATGA 1 cut(s) 1516
PciI ACATGT 3 cut(s) 1218, 1388, 1597
PctI GAATGC 1 cut(s) 1003
PdmI GAANNNNTTC 2 cut(s) 1590, 2413
PfeI GAWTC 8 cut(s) 169, 494, 945, 988, 1714, 1746, 2144, 2306
PkrI GCNGC 3 cut(s) 13, 760, 2507
PleI GAGTC 5 cut(s) 336, 880, 1354, 1909, 2564
PpsI GAGTC 5 cut(s) 336, 880, 1354, 1909, 2564
PscI ACATGT 3 cut(s) 1218, 1388, 1597
PshAI GACNNNNGTC 1 cut(s) 1600
PshBI ATTAAT 2 cut(s) 737, 2478
Psp124BI GAGCTC 1 cut(s) 99
Psp1406I AACGTT 1 cut(s) 1720
Psp6I CCWGG 2 cut(s) 33, 2294
PspGI CCWGG 2 cut(s) 33, 2294
PspN4I GGNNCC 3 cut(s) 85, 432, 1958
PspPI GGNCC 2 cut(s) 2237, 2535
PspXI VCTCGAGB 1 cut(s) 2390
PsuI RGATCY 3 cut(s) 1405, 1768, 2088
RsaI GTAC 4 cut(s) 306, 659, 1550, 1924
RsaNI GTAC 4 cut(s) 305, 658, 1549, 1923
RseI CAYNNNNRTG 3 cut(s) 288, 1490, 2196
SacI GAGCTC 1 cut(s) 99
SaqAI TTAA 6 cut(s) 342, 737, 908, 1497, 2478, 2519
SatI GCNGC 3 cut(s) 12, 759, 2506
Sau96I GGNCC 2 cut(s) 2237, 2535
ScaI AGTACT 1 cut(s) 1550
SchI GAGTC 5 cut(s) 337, 881, 1355, 1910, 2564
ScrFI CCNGG 2 cut(s) 35, 2296
SduI GDGCHC 3 cut(s) 88, 99, 462
SfaNI GCATC 4 cut(s) 136, 459, 1261, 1746
SfcI CTRYAG 2 cut(s) 1146, 1339
Sfr274I CTCGAG 2 cut(s) 1741, 2390
SfuI TTCGAA 1 cut(s) 250
SinI GGWCC 1 cut(s) 2535
SlaI CTCGAG 2 cut(s) 1741, 2390
SmiMI CAYNNNNRTG 3 cut(s) 288, 1490, 2196
SmlI CTYRAG 9 cut(s) 92, 107, 743, 1741, 1787, 1931, 2241, 2390, 2638
SmoI CTYRAG 9 cut(s) 92, 107, 743, 1741, 1787, 1931, 2241, 2390, 2638
SpeI ACTAGT 1 cut(s) 1351
SphI GCATGC 1 cut(s) 1168
SsiI CCGC 6 cut(s) 12, 1759, 1926, 2175, 2339, 2435
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 2 cut(s) 33, 2294
StyI CCWWGG 4 cut(s) 283, 463, 498, 613
TaaI ACNGT 6 cut(s) 337, 797, 1440, 1558, 2132, 2335
TaiI ACGT 3 cut(s) 33, 222, 1723
TaqI TCGA 7 cut(s) 222, 250, 1594, 1742, 1836, 2391, 2619
TaqII GACCGA 1 cut(s) 1206
TatI WGTACW 2 cut(s) 657, 1548
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 8 cut(s) 169, 494, 945, 988, 1714, 1746, 2144, 2306
Tru1I TTAA 6 cut(s) 342, 737, 908, 1497, 2478, 2519
Tru9I TTAA 6 cut(s) 342, 737, 908, 1497, 2478, 2519
TscAI CASTG 4 cut(s) 342, 577, 1780, 2461
TseFI GTSAC 1 cut(s) 1347
TseI GCWGC 2 cut(s) 758, 2505
Tsp45I GTSAC 1 cut(s) 1347
TspGWI ACGGA 2 cut(s) 884, 2600
TspRI CASTG 4 cut(s) 342, 577, 1780, 2461
VpaK11BI GGWCC 1 cut(s) 2535
VspI ATTAAT 2 cut(s) 737, 2478
XapI RAATTY 5 cut(s) 682, 1873, 1894, 2137, 2378
XbaI TCTAGA 2 cut(s) 1448, 1694
XceI RCATGY 8 cut(s) 1003, 1039, 1168, 1222, 1392, 1601, 1624, 1737
XhoI CTCGAG 2 cut(s) 1741, 2390
XmiI GTMKAC 1 cut(s) 2188
XmnI GAANNNNTTC 2 cut(s) 1590, 2413
ZraI GACGTC 1 cut(s) 220
ZrmI AGTACT 1 cut(s) 1550
Zsp2I ATGCAT 4 cut(s) 1005, 1276, 1506, 1739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.