Rh2CG270700

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000159
Physical Location & Seq
Forward (+)
29447087 .. 29448264
1178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG270700.1

Sequence Viewer

Length: 1026 bp
ATGTTCAAGTCAAAGAAATTGCGAACCATTCTAGTTCCTGAAGATGCCCAGATGAACCAATGTTTTGTGAAGACATGCATCTCGAGATTCAAATGTTTGCGGATGCTAGATCTCCGTTGGTCGGCTCTTGAGGAGCTACCAAGTTCCATTGGTAGTCTGTTTCATTTGAGATTTCTCAACTTATCTGAAAATAGGAAGATAAAAAGGCTCCCCAATTCCATCAGAAAGCTGCTGAATTTGGAGACCTTGCACCTTCGCAAGTGCAACGCACTTAAGGAGATACCCAAAGACATAGGAAACCTGATAAATCTCCGAAGCTTGGTGATAACTACACAACAGACATATTTGCCAAAAGGAATTAGACGCCTCACCTTACTTCGAGATTTATATTTTGTTGGATGTGTCAATCTTAAATCTTTAGGCGAAGAGATCCAATTCCTCAATAACCTTCATAAACTGCTGATTTATAGTTGTAATAATTTGGTATCCTTGCCACCAAATATGAAACACATGACTGCTTTAGATACTTTGGCTGTAATGGATTGTGAGAAGCTTCAGGTGATAATGAGATCAGGGGAAGGTCCTCGACGTCTTCGATCATTGGGTATCGGGAATTCAAGTTTGGAGGCTTTGCCCGCTTGGCTTGAAGATTCTGCAGACACTCTACAGAGTATATATATTTATGGATGTGATAATCTCACGGCACTTCCGGAGTTGATAAAGTTCAGATTCCTCGAGCAACTACACATTGGCGAATGCTCCAAATTGTCGGCTTTGTCGCAAGGGTTGCATTGCCTTACTGGGTTGAGAGGATTGACGATTTCAGGGTGTCCTAAATTGAGCAAAAGCTGCAAAAGGAAATTAAAAGGTGGGGAGTGGTCAAAGATGGCACGTCAGGTGAAGATTACACTCGACGCTGATGATGAAGAAGATGAAGCCGACAATCGCTTGGTCGACGGTAGGATCGTGTACCTCACAACGCGATCCAGCATGTATTGGGTGAGTATTTTGGGGTACAGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

341

Amino Acids

38.76

Weight (kDa)

9.33

Isoelectric Point (pI)

46.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 25 - 222 1.7e-26 Leucine-rich repeat region
LRR_13 PF23286 40 - 190 1.3e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_RPS2 PF23247 44 - 183 2.1e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_R13L1-DRL21 PF25019 194 - 253 4.6e-07 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 592
AccI GTMKAC 1 cut(s) 954
AccII CGCG 1 cut(s) 982
AccIII TCCGGA 1 cut(s) 709
AciI CCGC 2 cut(s) 100, 636
AclWI GGATC 3 cut(s) 424, 971, 978
AcsI RAATTY 2 cut(s) 235, 613
AcuI CTGAAG 2 cut(s) 60, 539
AcyI GRCGYC 2 cut(s) 364, 589
AfaI GTAC 2 cut(s) 971, 1016
AfiI CCNNNNNNNGG 2 cut(s) 121, 319
AflII CTTAAG 1 cut(s) 272
AgsI TTSAA 4 cut(s) 7, 91, 618, 647
AjiI CACGTC 1 cut(s) 893
AjuI GAANNNNNNNTTGG 2 cut(s) 605, 637
AluBI AGCT 5 cut(s) 136, 229, 318, 553, 849
AluI AGCT 5 cut(s) 136, 229, 318, 553, 849
Alw26I GTCTC 1 cut(s) 236
AlwI GGATC 3 cut(s) 424, 971, 978
Ama87I CYCGRG 2 cut(s) 82, 734
Aor13HI TCCGGA 1 cut(s) 709
ApeKI GCWGC 2 cut(s) 229, 849
ApoI RAATTY 2 cut(s) 235, 613
AspS9I GGNCC 1 cut(s) 581
AsuHPI GGTGA 5 cut(s) 334, 361, 571, 910, 1012
AvaI CYCGRG 2 cut(s) 82, 734
AvaII GGWCC 1 cut(s) 581
BaeI ACNNNNGTAYC 1 cut(s) 1006
BbsI GAAGAC 2 cut(s) 77, 584
BbvI GCAGC 2 cut(s) 216, 836
BccI CCATC 2 cut(s) 227, 880
BceAI ACGGC 1 cut(s) 717
BciVI GTATCC 1 cut(s) 496
BcoDI GTCTC 1 cut(s) 236
BfaI CTAG 2 cut(s) 32, 107
BfmI CTRYAG 2 cut(s) 654, 665
BfrI CTTAAG 1 cut(s) 272
BfuI GTATCC 1 cut(s) 496
BglI GCCNNNNNGGC 1 cut(s) 640
BglII AGATCT 1 cut(s) 109
BisI GCNGC 2 cut(s) 230, 850
BlsI GCNGC 2 cut(s) 231, 851
Bme18I GGWCC 1 cut(s) 581
BmeT110I CYCGRG 2 cut(s) 82, 734
BmgBI CACGTC 1 cut(s) 893
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 1 cut(s) 209
BmrI ACTGGG 1 cut(s) 810
BmsI GCATC 3 cut(s) 34, 87, 93
BmuI ACTGGG 1 cut(s) 810
BpiI GAAGAC 2 cut(s) 77, 584
BpuEI CTTGAG 1 cut(s) 149
BsaHI GRCGYC 2 cut(s) 364, 589
BsaI GGTCTC 1 cut(s) 236
BsaWI WCCGGW 1 cut(s) 709
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 319
Bse1I ACTGG 1 cut(s) 805
Bse3DI GCAATG 1 cut(s) 790
BseAI TCCGGA 1 cut(s) 709
BseGI GGATG 3 cut(s) 108, 404, 692
BseLI CCNNNNNNNGG 2 cut(s) 121, 319
BseMI GCAATG 1 cut(s) 790
BseNI ACTGG 1 cut(s) 805
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 2 cut(s) 216, 836
Bsh1236I CGCG 1 cut(s) 982
BsiHKCI CYCGRG 2 cut(s) 82, 734
BsiSI CCGG 1 cut(s) 710
BslI CCNNNNNNNGG 2 cut(s) 121, 319
BsmAI GTCTC 1 cut(s) 236
BsmI GAATGC 1 cut(s) 761
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 2 cut(s) 82, 734
Bsp13I TCCGGA 1 cut(s) 709
Bsp143I GATC 6 cut(s) 109, 429, 569, 596, 963, 983
BspACI CCGC 2 cut(s) 100, 636
BspEI TCCGGA 1 cut(s) 709
BspFNI CGCG 1 cut(s) 982
BspLI GGNNCC 1 cut(s) 209
BspMAI CTGCAG 1 cut(s) 658
BspPI GGATC 3 cut(s) 424, 971, 978
BspTI CTTAAG 1 cut(s) 272
BspTNI GGTCTC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 790
BsrI ACTGG 1 cut(s) 805
BssMI GATC 6 cut(s) 109, 429, 569, 596, 963, 983
BssNI GRCGYC 2 cut(s) 364, 589
Bst4CI ACNGT 1 cut(s) 959
Bst6I CTCTTC 1 cut(s) 420
BstACI GRCGYC 2 cut(s) 364, 589
BstAFI CTTAAG 1 cut(s) 272
BstAPI GCANNNNNTGC 2 cut(s) 787, 849
BstC8I GCNNGC 1 cut(s) 636
BstF5I GGATG 3 cut(s) 108, 404, 692
BstFNI CGCG 1 cut(s) 982
BstKTI GATC 6 cut(s) 112, 432, 572, 599, 966, 986
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 6 cut(s) 109, 429, 569, 596, 963, 983
BstMWI GCNNNNNNNGC 4 cut(s) 635, 640, 787, 849
BstNSI RCATGY 2 cut(s) 78, 994
BstSFI CTRYAG 2 cut(s) 654, 665
BstUI CGCG 1 cut(s) 982
BstV1I GCAGC 2 cut(s) 216, 836
BstV2I GAAGAC 2 cut(s) 77, 584
BstX2I RGATCY 2 cut(s) 109, 429
BstYI RGATCY 2 cut(s) 109, 429
BsuI GTATCC 1 cut(s) 496
BtrI CACGTC 1 cut(s) 893
BtsCI GGATG 3 cut(s) 108, 404, 692
Cac8I GCNNGC 1 cut(s) 636
Cfr13I GGNCC 1 cut(s) 581
CseI GACGC 2 cut(s) 372, 923
Csp6I GTAC 2 cut(s) 970, 1015
CviAII CATG 3 cut(s) 75, 511, 991
CviQI GTAC 2 cut(s) 970, 1015
DpnI GATC 6 cut(s) 111, 431, 571, 598, 965, 985
DpnII GATC 6 cut(s) 109, 429, 569, 596, 963, 983
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 1 cut(s) 581
Eco57I CTGAAG 2 cut(s) 60, 539
Eco88I CYCGRG 2 cut(s) 82, 734
EcoO109I RGGNCCY 1 cut(s) 581
EcoRI GAATTC 1 cut(s) 613
EcoT22I ATGCAT 1 cut(s) 80
FaeI CATG 3 cut(s) 78, 514, 994
FatI CATG 3 cut(s) 74, 510, 990
FauI CCCGC 1 cut(s) 643
FblI GTMKAC 1 cut(s) 954
Fnu4HI GCNGC 2 cut(s) 230, 850
FokI GGATG 3 cut(s) 115, 411, 699
Fsp4HI GCNGC 2 cut(s) 230, 850
FspBI CTAG 2 cut(s) 32, 107
GluI GCNGC 2 cut(s) 230, 850
HapII CCGG 1 cut(s) 710
HgaI GACGC 2 cut(s) 372, 923
Hin1I GRCGYC 2 cut(s) 364, 589
Hin1II CATG 3 cut(s) 78, 514, 994
HincII GTYRAC 1 cut(s) 955
HindII GTYRAC 1 cut(s) 955
HindIII AAGCTT 2 cut(s) 316, 551
HinfI GANTC 3 cut(s) 87, 650, 729
HpaII CCGG 1 cut(s) 710
HphI GGTGA 5 cut(s) 334, 361, 571, 910, 1012
Hpy166II GTNNAC 2 cut(s) 955, 970
Hpy188I TCNGA 4 cut(s) 187, 224, 314, 728
Hpy188III TCNNGA 7 cut(s) 38, 82, 84, 128, 380, 610, 710
Hpy8I GTNNAC 2 cut(s) 955, 970
Hpy99I CGWCG 3 cut(s) 591, 917, 959
HpyAV CCTTC 3 cut(s) 263, 458, 572
HpyCH4III ACNGT 1 cut(s) 959
HpyCH4IV ACGT 2 cut(s) 589, 892
HpyCH4V TGCA 6 cut(s) 78, 250, 264, 656, 790, 852
HpyF10VI GCNNNNNNNGC 4 cut(s) 635, 640, 787, 849
HpySE526I ACGT 2 cut(s) 589, 892
Hsp92I GRCGYC 2 cut(s) 364, 589
Hsp92II CATG 3 cut(s) 78, 514, 994
Kpn2I TCCGGA 1 cut(s) 709
Kzo9I GATC 6 cut(s) 109, 429, 569, 596, 963, 983
LmnI GCTCC 3 cut(s) 133, 213, 764
Lsp1109I GCAGC 2 cut(s) 216, 836
LweI GCATC 3 cut(s) 34, 87, 93
MaeI CTAG 2 cut(s) 32, 107
MaeII ACGT 2 cut(s) 589, 892
MalI GATC 6 cut(s) 111, 431, 571, 598, 965, 985
MboI GATC 6 cut(s) 109, 429, 569, 596, 963, 983
MboII GAAGA 9 cut(s) 53, 82, 208, 437, 584, 659, 913, 938, 941
MflI RGATCY 2 cut(s) 109, 429
MmeI TCCRAC 1 cut(s) 376
MnlI CCTC 8 cut(s) 124, 377, 449, 594, 619, 743, 803, 983
Mph1103I ATGCAT 1 cut(s) 80
MroI TCCGGA 1 cut(s) 709
MseI TTAA 3 cut(s) 273, 411, 863
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 1 cut(s) 710
Mva1269I GAATGC 1 cut(s) 761
MvnI CGCG 1 cut(s) 982
MwoI GCNNNNNNNGC 4 cut(s) 635, 640, 787, 849
NdeII GATC 6 cut(s) 109, 429, 569, 596, 963, 983
NlaIII CATG 3 cut(s) 78, 514, 994
NlaIV GGNNCC 1 cut(s) 209
NsiI ATGCAT 1 cut(s) 80
NspI RCATGY 2 cut(s) 78, 994
PaeR7I CTCGAG 2 cut(s) 82, 734
PcsI WCGNNNNNNNCGW 2 cut(s) 592, 963
PctI GAATGC 1 cut(s) 761
PfeI GAWTC 3 cut(s) 87, 650, 729
PkrI GCNGC 2 cut(s) 231, 851
PpuMI RGGWCCY 1 cut(s) 581
Psp5II RGGWCCY 1 cut(s) 581
PspN4I GGNNCC 1 cut(s) 209
PspPI GGNCC 1 cut(s) 581
PspPPI RGGWCCY 1 cut(s) 581
PspXI VCTCGAGB 1 cut(s) 734
PstI CTGCAG 1 cut(s) 658
PsuI RGATCY 2 cut(s) 109, 429
RsaI GTAC 2 cut(s) 971, 1016
RsaNI GTAC 2 cut(s) 970, 1015
SalI GTCGAC 1 cut(s) 953
SaqAI TTAA 3 cut(s) 273, 411, 863
SatI GCNGC 2 cut(s) 230, 850
Sau3AI GATC 6 cut(s) 109, 429, 569, 596, 963, 983
Sau96I GGNCC 1 cut(s) 581
SfaNI GCATC 3 cut(s) 34, 87, 93
SfcI CTRYAG 2 cut(s) 654, 665
Sfr274I CTCGAG 2 cut(s) 82, 734
SinI GGWCC 1 cut(s) 581
SlaI CTCGAG 2 cut(s) 82, 734
SmlI CTYRAG 4 cut(s) 82, 128, 272, 734
SmoI CTYRAG 4 cut(s) 82, 128, 272, 734
SsiI CCGC 2 cut(s) 100, 636
SspMI CTAG 2 cut(s) 32, 107
TaaI ACNGT 1 cut(s) 959
TaiI ACGT 2 cut(s) 592, 895
TaqI TCGA 7 cut(s) 83, 379, 586, 595, 735, 912, 954
TfiI GAWTC 3 cut(s) 87, 650, 729
Tru1I TTAA 3 cut(s) 273, 411, 863
Tru9I TTAA 3 cut(s) 273, 411, 863
TseI GCWGC 2 cut(s) 229, 849
TspDTI ATGAA 6 cut(s) 68, 152, 440, 518, 939, 948
TspGWI ACGGA 1 cut(s) 104
Vha464I CTTAAG 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 581
XapI RAATTY 2 cut(s) 235, 613
XceI RCATGY 2 cut(s) 78, 994
XhoI CTCGAG 2 cut(s) 82, 734
XmiI GTMKAC 1 cut(s) 954
XspI CTAG 2 cut(s) 32, 107
ZraI GACGTC 1 cut(s) 590
Zsp2I ATGCAT 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.