RchiOBHm_Chr5g0009011

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000159
Physical Location & Seq
Reverse (-)
5959345 .. 5961357
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28993

Sequence Viewer

Length: 1242 bp
ATGTGGATGGCACAAGGCTTCCTTCACTCCTCTACCAGAAATGAACAAGATTTTGAAGAGATTGGCCTAGATTACATGAGGCAGCTATGTTCTAGATCTTTCTTTCACATTGAAGAAGATGATTTCTTTTGTATGAGGTTTAAGATGCATGATTTAATTCATGATTTAGCCATCTTGGTGGCACAGGTAGAGTACTTATCTGTCAATTTTCATCAATCTGGTTCTTTTGATAGGGTCCGGCACCTGTCAATATATACAAAGGACTTGTCTGACAATGAGGAAGTCCCTGATTTCATACTCCAGTTAGGCAAGGTGAGAACCATTCTCATCCCAGAAGAGAAGGTTGGCATCACTGGTCGATCTTTCATAAAAAAATGCATTTCCAGATTCAAATATTTGCGGTTGCTAAATCTGCGTAGCTCAACTTTCGACGAGTTGCCAAGTTCCATTGGTAACTTGAGTCACTTGAGATATGTGGACCTATCATACAATTGCCACATAAAAAGGGTTCCTGATTGCATTTGCAAGCTGCAGCATCTACAAACCTTATTGCTTTCACATTGTGAGGAGCTTGAGAAGTTTCCCAAGGACATAGGGAACCTGATTAGCCTCAGGTACTTGGCACTAACTACAAAGAAAACTTGTTTGCCAACAGGAATCGATCGTCTCACTTCTCTTCGCATTTTACATGTTGTTGCATGCCGAAACCTTCTTTCTTTGGAAGGGTTGCCCCGCCTCACTCACCTCAAAATGTTCATTATTGAGGAATGTCCAGTTCTGAGGTCTTTGCCGCATAACATGAAATATTCAACTGCATTAGAGACTTTAATCATCTCTGACTGTGAGAAACTTGATCTGTTGAGTCTTGAAGAATGCGTTCGAGGTCTAAGATCATTCTGGATCTTGAAATCAAAGTTGAAGAACTTTCCCCTTTGGCTTAAAGAGTGTGCAAGTACTCTACAAAACATATGTATTGCAAACTGTCAGAATCTCATTGCACTTCCGGAGTGGCTGCAAAGCTTCAAGTTGCTTGAAAAGCTAGTGATTGAAAACTGCCCCCAAGTGCCAGCTTTGCCAGAGGGGATGCATTGCCTCACAACATTGAGAGAATTGAAGATCAGTGGGTGTCCTCGATTGATGGAAAGATGCAACAGGCACGGAGGTATAGATTGGTTCAAAATTGCTCATGTCACAAAGATAATGATTGGAGGTGTCAACAACAACACCTTCGGTGGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

413

Amino Acids

47.68

Weight (kDa)

8.21

Isoelectric Point (pI)

60.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 1 - 57 2.4e-15 Disease resistance protein Winged helix domain
LRR_14 PF23598 126 - 371 7.4e-22 Leucine-rich repeat region
LRR_8 PF13855 133 - 188 2.4e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 240
AccIII TCCGGA 1 cut(s) 1003
AciI CCGC 3 cut(s) 400, 733, 791
AclWI GGATC 1 cut(s) 908
AdeI CACNNNGTG 1 cut(s) 563
AfaI GTAC 3 cut(s) 194, 617, 955
AflIII ACRYGT 1 cut(s) 688
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
AluBI AGCT 7 cut(s) 85, 420, 529, 571, 1020, 1039, 1070
AluI AGCT 7 cut(s) 85, 420, 529, 571, 1020, 1039, 1070
Alw26I GTCTC 2 cut(s) 671, 815
AlwI GGATC 1 cut(s) 908
Aor13HI TCCGGA 1 cut(s) 1003
AoxI GGCC 1 cut(s) 64
ApeKI GCWGC 4 cut(s) 82, 529, 532, 1012
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
Asp700I GAANNNNTTC 1 cut(s) 876
AspS9I GGNCC 2 cut(s) 235, 478
AsuHPI GGTGA 2 cut(s) 325, 734
AvaII GGWCC 2 cut(s) 235, 478
AxyI CCTNAGG 1 cut(s) 611
BanI GGYRCC 1 cut(s) 240
BbvI GCAGC 4 cut(s) 94, 516, 544, 999
BccI CCATC 2 cut(s) 179, 1132
BcoDI GTCTC 2 cut(s) 671, 815
BfaI CTAG 3 cut(s) 68, 93, 1040
BfmI CTRYAG 1 cut(s) 530
BglII AGATCT 1 cut(s) 95
BisI GCNGC 5 cut(s) 83, 530, 533, 791, 1013
BlsI GCNGC 5 cut(s) 84, 531, 534, 792, 1014
BmcAI AGTACT 2 cut(s) 194, 955
Bme18I GGWCC 2 cut(s) 235, 478
BmgT120I GGNCC 2 cut(s) 235, 478
BmiI GGNNCC 4 cut(s) 236, 242, 510, 599
BmsI GCATC 5 cut(s) 135, 357, 544, 1074, 1136
BpmI CTGGAG 1 cut(s) 284
BpuEI CTTGAG 3 cut(s) 478, 487, 593
Bsa29I ATCGAT 1 cut(s) 660
BsaJI CCNNGG 1 cut(s) 585
BsaWI WCCGGW 1 cut(s) 1003
Bse1I ACTGG 3 cut(s) 301, 358, 773
Bse21I CCTNAGG 1 cut(s) 611
Bse3DI GCAATG 2 cut(s) 993, 1087
BseAI TCCGGA 1 cut(s) 1003
BseCI ATCGAT 1 cut(s) 660
BseDI CCNNGG 1 cut(s) 585
BseGI GGATG 3 cut(s) 12, 327, 1089
BseMI GCAATG 2 cut(s) 993, 1087
BseMII CTCAG 2 cut(s) 625, 770
BseNI ACTGG 3 cut(s) 301, 358, 773
BseRI GAGGAG 2 cut(s) 19, 581
BseXI GCAGC 4 cut(s) 94, 516, 544, 999
Bsh1285I CGRYCG 1 cut(s) 664
BshFI GGCC 1 cut(s) 66
BshNI GGYRCC 1 cut(s) 240
BshVI ATCGAT 1 cut(s) 660
BsiEI CGRYCG 1 cut(s) 664
BsiSI CCGG 2 cut(s) 238, 1004
BslFI GGGAC 1 cut(s) 269
BsmAI GTCTC 2 cut(s) 671, 815
BsmBI CGTCTC 1 cut(s) 671
BsmFI GGGAC 1 cut(s) 269
BsmI GAATGC 1 cut(s) 878
BsnI GGCC 1 cut(s) 66
Bsp13I TCCGGA 1 cut(s) 1003
Bsp143I GATC 7 cut(s) 95, 359, 661, 853, 890, 900, 1116
BspACI CCGC 3 cut(s) 400, 733, 791
BspANI GGCC 1 cut(s) 66
BspCNI CTCAG 2 cut(s) 624, 771
BspDI ATCGAT 1 cut(s) 660
BspEI TCCGGA 1 cut(s) 1003
BspHI TCATGA 1 cut(s) 160
BspLI GGNNCC 4 cut(s) 236, 242, 510, 599
BspMAI CTGCAG 1 cut(s) 534
BspPI GGATC 1 cut(s) 908
BspT107I GGYRCC 1 cut(s) 240
BsrDI GCAATG 2 cut(s) 993, 1087
BsrI ACTGG 3 cut(s) 301, 358, 773
BssECI CCNNGG 1 cut(s) 585
BssMI GATC 7 cut(s) 95, 359, 661, 853, 890, 900, 1116
BssT1I CCWWGG 1 cut(s) 585
Bst4CI ACNGT 2 cut(s) 842, 983
Bst6I CTCTTC 3 cut(s) 51, 330, 681
BstC8I GCNNGC 3 cut(s) 527, 700, 1068
BstDEI CTNAG 3 cut(s) 611, 779, 887
BstF5I GGATG 3 cut(s) 12, 327, 1089
BstKTI GATC 7 cut(s) 98, 362, 664, 856, 893, 903, 1119
BstMAI GTCTC 2 cut(s) 671, 815
BstMBI GATC 7 cut(s) 95, 359, 661, 853, 890, 900, 1116
BstMCI CGRYCG 1 cut(s) 664
BstMWI GCNNNNNNNGC 3 cut(s) 412, 1036, 1072
BstNSI RCATGY 2 cut(s) 692, 702
BstSFI CTRYAG 1 cut(s) 530
BstV1I GCAGC 4 cut(s) 94, 516, 544, 999
BstX2I RGATCY 2 cut(s) 95, 900
BstXI CCANNNNNNTGG 1 cut(s) 178
BstYI RGATCY 2 cut(s) 95, 900
Bsu15I ATCGAT 1 cut(s) 660
Bsu36I CCTNAGG 1 cut(s) 611
BsuRI GGCC 1 cut(s) 66
BsuTUI ATCGAT 1 cut(s) 660
BtsCI GGATG 3 cut(s) 12, 327, 1089
BtsIMutI CAGTG 2 cut(s) 351, 1126
Cac8I GCNNGC 3 cut(s) 527, 700, 1068
CciI TCATGA 1 cut(s) 160
Cfr13I GGNCC 2 cut(s) 235, 478
ClaI ATCGAT 1 cut(s) 660
Csp6I GTAC 3 cut(s) 193, 616, 954
CviAII CATG 7 cut(s) 76, 149, 161, 689, 699, 799, 1187
CviQI GTAC 3 cut(s) 193, 616, 954
DdeI CTNAG 3 cut(s) 611, 779, 887
DpnI GATC 7 cut(s) 97, 361, 663, 855, 892, 902, 1118
DpnII GATC 7 cut(s) 95, 359, 661, 853, 890, 900, 1116
DraIII CACNNNGTG 1 cut(s) 563
Eam1104I CTCTTC 3 cut(s) 51, 330, 681
EarI CTCTTC 3 cut(s) 51, 330, 681
Eco130I CCWWGG 1 cut(s) 585
Eco47I GGWCC 2 cut(s) 235, 478
Eco81I CCTNAGG 1 cut(s) 611
EcoT14I CCWWGG 1 cut(s) 585
EcoT22I ATGCAT 3 cut(s) 150, 380, 1089
ErhI CCWWGG 1 cut(s) 585
Esp3I CGTCTC 1 cut(s) 671
FaeI CATG 7 cut(s) 79, 152, 164, 692, 702, 802, 1190
FalI AAGNNNNNCTT 1 cut(s) 38
FaqI GGGAC 1 cut(s) 269
FatI CATG 7 cut(s) 75, 148, 160, 688, 698, 798, 1186
FauI CCCGC 1 cut(s) 740
FauNDI CATATG 1 cut(s) 968
Fnu4HI GCNGC 5 cut(s) 83, 530, 533, 791, 1013
FokI GGATG 3 cut(s) 19, 314, 1096
Fsp4HI GCNGC 5 cut(s) 83, 530, 533, 791, 1013
FspBI CTAG 3 cut(s) 68, 93, 1040
GluI GCNGC 5 cut(s) 83, 530, 533, 791, 1013
GsuI CTGGAG 1 cut(s) 284
HaeIII GGCC 1 cut(s) 66
HapII CCGG 2 cut(s) 238, 1004
Hin1II CATG 7 cut(s) 79, 152, 164, 692, 702, 802, 1190
HincII GTYRAC 1 cut(s) 1216
HindII GTYRAC 1 cut(s) 1216
HindIII AAGCTT 1 cut(s) 1018
HinfI GANTC 5 cut(s) 387, 460, 657, 862, 988
HpaII CCGG 2 cut(s) 238, 1004
HphI GGTGA 2 cut(s) 325, 734
Hpy166II GTNNAC 2 cut(s) 478, 1216
Hpy188I TCNGA 4 cut(s) 271, 780, 838, 987
Hpy188III TCNNGA 8 cut(s) 93, 161, 384, 512, 866, 898, 904, 1004
Hpy8I GTNNAC 2 cut(s) 478, 1216
Hpy99I CGWCG 1 cut(s) 434
HpyAV CCTTC 5 cut(s) 32, 334, 716, 719, 1237
HpyCH4III ACNGT 2 cut(s) 842, 983
HpyF10VI GCNNNNNNNGC 3 cut(s) 412, 1036, 1072
HpyF3I CTNAG 3 cut(s) 611, 779, 887
Hsp92II CATG 7 cut(s) 79, 152, 164, 692, 702, 802, 1190
Kpn2I TCCGGA 1 cut(s) 1003
Kzo9I GATC 7 cut(s) 95, 359, 661, 853, 890, 900, 1116
LmnI GCTCC 1 cut(s) 568
Lsp1109I GCAGC 4 cut(s) 94, 516, 544, 999
LweI GCATC 5 cut(s) 135, 357, 544, 1074, 1136
MaeI CTAG 3 cut(s) 68, 93, 1040
MaeIII GTNAC 3 cut(s) 452, 461, 1189
MalI GATC 7 cut(s) 97, 361, 663, 855, 892, 902, 1118
MboI GATC 7 cut(s) 95, 359, 661, 853, 890, 900, 1116
MboII GAAGA 8 cut(s) 68, 125, 128, 347, 668, 881, 931, 1126
MfeI CAATTG 1 cut(s) 490
MflI RGATCY 2 cut(s) 95, 900
MluCI AATT 5 cut(s) 156, 205, 490, 1109, 1179
MlyI GAGTC 2 cut(s) 469, 871
Mph1103I ATGCAT 3 cut(s) 150, 380, 1089
MroI TCCGGA 1 cut(s) 1003
MroXI GAANNNNTTC 1 cut(s) 876
MseI TTAA 4 cut(s) 141, 155, 827, 939
MslI CAYNNNNRTG 1 cut(s) 176
MspI CCGG 2 cut(s) 238, 1004
MunI CAATTG 1 cut(s) 490
Mva1269I GAATGC 1 cut(s) 878
MwoI GCNNNNNNNGC 3 cut(s) 412, 1036, 1072
NdeI CATATG 1 cut(s) 968
NdeII GATC 7 cut(s) 95, 359, 661, 853, 890, 900, 1116
NlaIII CATG 7 cut(s) 79, 152, 164, 692, 702, 802, 1190
NlaIV GGNNCC 4 cut(s) 236, 242, 510, 599
NmuCI GTSAC 2 cut(s) 461, 1189
NsiI ATGCAT 3 cut(s) 150, 380, 1089
NspI RCATGY 2 cut(s) 692, 702
PaeI GCATGC 1 cut(s) 702
PagI TCATGA 1 cut(s) 160
PciI ACATGT 1 cut(s) 688
PctI GAATGC 1 cut(s) 878
PdmI GAANNNNTTC 1 cut(s) 876
PfeI GAWTC 3 cut(s) 387, 657, 988
PkrI GCNGC 5 cut(s) 84, 531, 534, 792, 1014
Ple19I CGATCG 1 cut(s) 664
PleI GAGTC 2 cut(s) 468, 870
PpsI GAGTC 2 cut(s) 468, 870
PscI ACATGT 1 cut(s) 688
PspN4I GGNNCC 4 cut(s) 236, 242, 510, 599
PspPI GGNCC 2 cut(s) 235, 478
PstI CTGCAG 1 cut(s) 534
PsuI RGATCY 2 cut(s) 95, 900
PvuI CGATCG 1 cut(s) 664
RsaI GTAC 3 cut(s) 194, 617, 955
RsaNI GTAC 3 cut(s) 193, 616, 954
RseI CAYNNNNRTG 1 cut(s) 176
SaqAI TTAA 4 cut(s) 141, 155, 827, 939
SatI GCNGC 5 cut(s) 83, 530, 533, 791, 1013
Sau3AI GATC 7 cut(s) 95, 359, 661, 853, 890, 900, 1116
Sau96I GGNCC 2 cut(s) 235, 478
ScaI AGTACT 2 cut(s) 194, 955
SchI GAGTC 2 cut(s) 469, 871
SfaNI GCATC 5 cut(s) 135, 357, 544, 1074, 1136
SfcI CTRYAG 1 cut(s) 530
SinI GGWCC 2 cut(s) 235, 478
SmiMI CAYNNNNRTG 1 cut(s) 176
SmlI CTYRAG 3 cut(s) 457, 466, 572
SmoI CTYRAG 3 cut(s) 457, 466, 572
SphI GCATGC 1 cut(s) 702
Sse9I AATT 5 cut(s) 156, 205, 490, 1109, 1179
SsiI CCGC 3 cut(s) 400, 733, 791
SspI AATATT 2 cut(s) 395, 806
SspMI CTAG 3 cut(s) 68, 93, 1040
StyI CCWWGG 1 cut(s) 585
TaaI ACNGT 2 cut(s) 842, 983
TaqI TCGA 5 cut(s) 358, 429, 660, 880, 1132
TasI AATT 5 cut(s) 156, 205, 490, 1109, 1179
TatI WGTACW 2 cut(s) 192, 953
TauI GCSGC 1 cut(s) 793
TfiI GAWTC 3 cut(s) 387, 657, 988
Tru1I TTAA 4 cut(s) 141, 155, 827, 939
Tru9I TTAA 4 cut(s) 141, 155, 827, 939
TscAI CASTG 2 cut(s) 358, 1126
TseFI GTSAC 2 cut(s) 461, 1189
TseI GCWGC 4 cut(s) 82, 529, 532, 1012
Tsp45I GTSAC 2 cut(s) 461, 1189
TspDTI ATGAA 7 cut(s) 57, 149, 200, 283, 355, 745, 815
TspGWI ACGGA 1 cut(s) 1173
TspRI CASTG 2 cut(s) 358, 1126
VpaK11BI GGWCC 2 cut(s) 235, 478
XbaI TCTAGA 1 cut(s) 92
XceI RCATGY 2 cut(s) 692, 702
XmnI GAANNNNTTC 1 cut(s) 876
XspI CTAG 3 cut(s) 68, 93, 1040
ZrmI AGTACT 2 cut(s) 194, 955
Zsp2I ATGCAT 3 cut(s) 150, 380, 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.