RchiOBHm_Chr2g0116761

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000159
Physical Location & Seq
Forward (+)
28999546 .. 29002227
2682 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48978

Sequence Viewer

Length: 2682 bp
ATGGAGTTTGCCACCAGCATTGCAGAGAACGTCTTGGGTAGGCTAGCTTCCTATGCTTCCCAAGAGGTCTCCTTGGCATGGGGTGCCCAACTTGAGCTCACCAAGCTCAACAAGACCTTTTCTACCATCAAACTAGTGCTTCAGGATGCAGAGAAGAAGCAAGTGAAGAATCCCCTAATCACTCGTTGGTTGGGAGATCTCAAAGATGTTTGCCATGACATTGACGATGTCCTTGATGAACTAGAGTTCCAAAAGCTGCGGAAGAAAGTTCTAGTCAACAACAGTGCGAGAGTCAAAGGACAGGTATGCCAATTTTTTTCGCGTTGGAATCCAGTTGTGCTTAACTTCAAGTTGGGACATAAAGTGAAAGAAATTAGAGAACGACTAGTTGAAATTGAAAAAGACAAGAGAGAGTTCTCTCTTGTTGAAGTTGCTGAGCAAGCCGAAGATCATCATGCACCCCAACAAGTGCACGATAACAGGAGGATGACTAGCTCCAAAGTTGACGCTTCAAAAGTTATTGGAAGAGATGATAATAAAAAACAGATTATCAAGCATCTCTTGAATGACACTGATTTCTCCAGTGAGGAGAATGTTTCTGTTGTTTCCATAATTGGGTTAGGAGGGTTGGGGAAAACAACACTTGCTGAATTGGTGTATAATGATAGCATGGTACAAGAAAATTTTGAGAAAAAGATGTGGATATGCGTCTCAGACAACTTTGATATTCAAGCATTAATTCGAGGTATTACTAATGCTGCAAATGGACCAAAATGTGAGGATGAAAGTTTGGATCTTATGGAAAGAAAGTTGCAAGATACTCTTAGGGGCAAAAAAATTTTATTGGTGTTGGATGATATTTGGGATACAGAGTTGATTGGAGTAACAATTAAAAAATGGACTGATTTAAAGCTTTTGTTAAATGTGGGAGCTAATGGAAGCAAAATCATCATAACAACACGAAATGAATCAGTTGCTTTACTTGTGAGTTCCTTATACATGCATTCTTTAAAAGGTCTTTCCCACAATGATTGCATGTCCTTGTTCATCCAAAGGGCGTTTAAAATAGGAGAGGAGCAGCGCTATAAGCATTTGATAGAAATTGGAGAGGATATTGTGAAGAAGTGTGGAGGACTTCCTTTAGCAGTTGCAGCTATAGGGGGCATGCTCTATTTGAATAAAGAGCAACGTCATTGGTCTAGTGTCAGAGATGATGACATGTGGAGTATAGGGAATGACAATATTCTACCTGCACTCAAATTGAGCTATGATGCATTACCACAAAAGTTGAAACCATGTTTTGCATATTGTTCACTTTTTCCAAAGGATTATGTATTCGACTGTGAACATTTGGTTCCATTGTGGATATCACAAGGCTACCTTAAAACATGCAAGAAAAATGAAGATTTGGAACAGATGGGTTTAGAGTACGTAAGGGAGTTTTGTTCTAGATCTTTGTTTCAAGTCGATGTGGATTGGAAAACAACCTTACGTATTAAAATACATGATCTAGTTCATGATTTAGCTATTAAAGTGGCACAAGTAGAGTACTCCACAGTCAATTTTCGTCCCTCTAGTGGCTTTGAAGTGTTTCGACATGTGTCAATATTCAAAAAAGACTTGCTTGAAGAAGGGGCACAAGTTCCCGATTTCATGCTCAAGTCAAAGAAATTACGAACCATTCTAATTCCTGAAGAGAAATTACGAACCATTCTAATTCCTGAAGATGGCCAGATAAATCAACGTTGTTTTGTGAAGACATGCATCTCAAGATTCAAATATATGCGGTTGCTAGGTCTGGAGGGGTTGCTTCTTGATGAGCTACCAAGTTCCATTGGTAGTTTGCTTCATTTGAGATTCCTCGACTTGTCTTTTAATAGTAAGTTAAAAAGGCTTCCCAATTCCATCAGTAAGCTGCTGAATTTGGAGTACTTGGACTTTGAGGAGTGCAACGCACTTGAGGAGATACCCAAAGACATAGGGAACCTGATCAACCTACGAAGCCTGACGATGACAACACAGCAAACGTATTTGCCAGAAGGAATTAGACGCCTCACCTCACTTCGATATTTAGGTTTTGTAGGATGTGTCAATCTTAAATCTTTTGGCGAAGAGATCGAATTCCTCAACAACCTTCGCAATTTGTGGATTAACAGGTGTAATAATTTGGAATGCTTGCTACCAAATATGAAACACATGACCGCTTTAGATTCTTTGTTCATCGAAGATTGCAAAAAGCTTCAGTTGATGCTGAGATCAGGGGAAGGTCCTCAAAGTCTTCGATCATTGGTTATCTGGAATTCAAGTTTGGAGGCTTTGCCCCCTTGGCTCACTGAAGATTCTGCAGATACTCTACAGAGCATAGCACTTGATCGATGTCATAATCTCACGGCACTTCCGGAGTTGATGAACTTCAAATTCCTCGAGCAACTACGCATTGAAGAATGCTCCAAATTGTCGGCTTTGCCGCAAGGGTTGCATTGCCTTACTCATTTGAGAGAACTGTACATTGAAGACTGTCCTGAATTGAGCAAAAGCTGCGTAAGGCAATTAAAAGGTGAGGAGTTGTCGAAGATGGGACGTCACGTGACGATTACGCTAGACGCTGATGATGAAGAAGATGAAGCCGACAATCGCTTGGCCGACGGTAGGATCAGGTACCACCCGCGACGACGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

893

Amino Acids

102.38

Weight (kDa)

6.25

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 94 2.2e-20 Rx N-terminal domain
NB-ARC PF00931 177 - 355 8.6e-31 NB-ARC domain
WHD_DRP PF23559 439 - 509 1.4e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 588 - 789 7.4e-26 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 757 - 816 2.9e-08 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2584
Acc36I ACCTGC 1 cut(s) 1258
Acc65I GGTACC 1 cut(s) 2658
AccB1I GGYRCC 2 cut(s) 83, 2658
AccII CGCG 2 cut(s) 322, 2668
AccIII TCCGGA 1 cut(s) 2398
AciI CCGC 5 cut(s) 259, 1786, 2202, 2468, 2666
AclI AACGTT 1 cut(s) 1744
AclWI GGATC 2 cut(s) 801, 2660
AcoI YGGCCR 2 cut(s) 1729, 2640
AcsI RAATTY 6 cut(s) 682, 837, 1921, 2120, 2299, 2417
AcuI CTGAAG 5 cut(s) 125, 1713, 1743, 2225, 2355
AcvI CACGTG 1 cut(s) 2587
AcyI GRCGYC 3 cut(s) 2050, 2581, 2674
AfaI GTAC 6 cut(s) 675, 1430, 1550, 1931, 2507, 2660
AfeI AGCGCT 1 cut(s) 1082
AfiI CCNNNNNNNGG 5 cut(s) 78, 615, 1577, 1727, 2649
AflIII ACRYGT 2 cut(s) 1218, 1597
AhlI ACTAGT 2 cut(s) 133, 385
AjuI GAANNNNNNNTTGG 2 cut(s) 2291, 2323
AloI GAACNNNNNNTCC 2 cut(s) 231, 263
Alw21I GWGCWC 2 cut(s) 99, 474
Alw26I GTCTC 2 cut(s) 73, 715
Alw44I GTGCAC 1 cut(s) 470
AlwI GGATC 2 cut(s) 801, 2660
Ama87I CYCGRG 1 cut(s) 2423
Aor13HI TCCGGA 1 cut(s) 2398
Aor51HI AGCGCT 1 cut(s) 1082
AoxI GGCC 2 cut(s) 1729, 2640
ApaLI GTGCAC 1 cut(s) 470
ApeKI GCWGC 6 cut(s) 256, 758, 1078, 1151, 1915, 2538
ApoI RAATTY 6 cut(s) 682, 837, 1921, 2120, 2299, 2417
AseI ATTAAT 1 cut(s) 737
Asp700I GAANNNNTTC 1 cut(s) 1590
Asp718I GGTACC 1 cut(s) 2658
AspLEI GCGC 1 cut(s) 1083
AspS9I GGNCC 2 cut(s) 767, 2267
AsuHPI GGTGA 3 cut(s) 91, 2047, 2570
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 2423
AvaII GGWCC 2 cut(s) 767, 2267
BaeGI GKGCMC 3 cut(s) 88, 474, 1639
BalI TGGCCA 1 cut(s) 1731
BanI GGYRCC 2 cut(s) 83, 2658
BanII GRGCYC 1 cut(s) 99
BarI GAAGNNNNNNTAC 4 cut(s) 31, 63, 1686, 1718
BbrPI CACGTG 1 cut(s) 2587
BbsI GAAGAC 3 cut(s) 1763, 2270, 2520
Bbv12I GWGCWC 2 cut(s) 99, 474
BbvI GCAGC 6 cut(s) 243, 745, 1090, 1163, 1902, 2525
BccI CCATC 5 cut(s) 134, 1411, 1721, 1913, 2569
BceAI ACGGC 1 cut(s) 2406
BciVI GTATCC 1 cut(s) 859
BclI TGATCA 1 cut(s) 1989
BcoDI GTCTC 2 cut(s) 73, 715
BcuI ACTAGT 2 cut(s) 133, 385
BfmI CTRYAG 3 cut(s) 1155, 2343, 2354
BfoI RGCGCY 1 cut(s) 1084
BfuAI ACCTGC 1 cut(s) 1258
BfuI GTATCC 1 cut(s) 859
BglI GCCNNNNNGGC 1 cut(s) 2326
BglII AGATCT 2 cut(s) 196, 1451
BisI GCNGC 7 cut(s) 257, 759, 1079, 1152, 1916, 2468, 2539
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 7 cut(s) 258, 760, 1080, 1153, 1917, 2469, 2540
BmcAI AGTACT 2 cut(s) 1550, 1931
Bme18I GGWCC 2 cut(s) 767, 2267
BmeT110I CYCGRG 1 cut(s) 2423
BmgT120I GGNCC 2 cut(s) 767, 2267
BmiI GGNNCC 4 cut(s) 85, 1356, 1985, 2660
BmsI GCATC 5 cut(s) 136, 565, 1261, 1773, 2238
BmtI GCTAGC 1 cut(s) 47
BoxI GACNNNNGTC 1 cut(s) 1600
BpiI GAAGAC 3 cut(s) 1763, 2270, 2520
BpmI CTGGAG 2 cut(s) 565, 1820
Bpu1102I GCTNAGC 1 cut(s) 435
BpuEI CTTGAG 4 cut(s) 113, 1643, 1753, 1979
Bsa29I ATCGAT 1 cut(s) 2374
BsaAI YACGTR 3 cut(s) 1432, 1493, 2587
BsaHI GRCGYC 3 cut(s) 2050, 2581, 2674
BsaI GGTCTC 1 cut(s) 73
BsaJI CCNNGG 2 cut(s) 72, 2324
BsaWI WCCGGW 1 cut(s) 2398
Bsc4I CCNNNNNNNGG 5 cut(s) 78, 615, 1577, 1727, 2649
Bse1I ACTGG 2 cut(s) 332, 582
Bse3DI GCAATG 2 cut(s) 18, 2479
BseAI TCCGGA 1 cut(s) 2398
BseCI ATCGAT 1 cut(s) 2374
BseDI CCNNGG 2 cut(s) 72, 2324
BseGI GGATG 6 cut(s) 151, 492, 787, 859, 1047, 2090
BseLI CCNNNNNNNGG 5 cut(s) 78, 615, 1577, 1727, 2649
BseMI GCAATG 2 cut(s) 18, 2479
BseMII CTCAG 3 cut(s) 426, 726, 2243
BseNI ACTGG 2 cut(s) 332, 582
BseRI GAGGAG 5 cut(s) 602, 1088, 1958, 1976, 2576
BseSI GKGCMC 3 cut(s) 88, 474, 1639
BseXI GCAGC 6 cut(s) 243, 745, 1090, 1163, 1902, 2525
BsgI GTGCAG 1 cut(s) 1236
Bsh1236I CGCG 2 cut(s) 322, 2668
BshFI GGCC 2 cut(s) 1731, 2642
BshNI GGYRCC 2 cut(s) 83, 2658
BshVI ATCGAT 1 cut(s) 2374
BsiHKAI GWGCWC 2 cut(s) 99, 474
BsiHKCI CYCGRG 1 cut(s) 2423
BsiSI CCGG 1 cut(s) 2399
BslFI GGGAC 3 cut(s) 369, 1554, 2592
BslI CCNNNNNNNGG 5 cut(s) 78, 615, 1577, 1727, 2649
BsmAI GTCTC 2 cut(s) 73, 715
BsmBI CGTCTC 1 cut(s) 715
BsmFI GGGAC 3 cut(s) 369, 1554, 2592
BsmI GAATGC 3 cut(s) 1003, 2177, 2450
BsnI GGCC 2 cut(s) 1731, 2642
Bso31I GGTCTC 1 cut(s) 73
BsoBI CYCGRG 1 cut(s) 2423
Bsp1286I GDGCHC 4 cut(s) 88, 99, 474, 1639
Bsp13I TCCGGA 1 cut(s) 2398
Bsp1407I TGTACA 1 cut(s) 2505
Bsp1720I GCTNAGC 1 cut(s) 435
BspACI CCGC 5 cut(s) 259, 1786, 2202, 2468, 2666
BspANI GGCC 2 cut(s) 1731, 2642
BspCNI CTCAG 3 cut(s) 427, 725, 2244
BspDI ATCGAT 1 cut(s) 2374
BspEI TCCGGA 1 cut(s) 2398
BspFNI CGCG 2 cut(s) 322, 2668
BspHI TCATGA 1 cut(s) 1516
BspLI GGNNCC 4 cut(s) 85, 1356, 1985, 2660
BspMAI CTGCAG 1 cut(s) 2347
BspMI ACCTGC 1 cut(s) 1258
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 2 cut(s) 801, 2660
BspT107I GGYRCC 2 cut(s) 83, 2658
BspTNI GGTCTC 1 cut(s) 73
BsrDI GCAATG 2 cut(s) 18, 2479
BsrGI TGTACA 1 cut(s) 2505
BsrI ACTGG 2 cut(s) 332, 582
BssECI CCNNGG 2 cut(s) 72, 2324
BssNI GRCGYC 3 cut(s) 2050, 2581, 2674
BssT1I CCWWGG 2 cut(s) 72, 2324
Bst4CI ACNGT 6 cut(s) 284, 1343, 1558, 2505, 2519, 2648
Bst6I CTCTTC 3 cut(s) 520, 1689, 2106
BstACI GRCGYC 3 cut(s) 2050, 2581, 2674
BstAPI GCANNNNNTGC 3 cut(s) 83, 2476, 2538
BstAUI TGTACA 1 cut(s) 2505
BstBAI YACGTR 3 cut(s) 1432, 1493, 2587
BstC8I GCNNGC 4 cut(s) 45, 441, 1166, 2177
BstDEI CTNAG 4 cut(s) 435, 712, 824, 2252
BstF5I GGATG 6 cut(s) 151, 492, 787, 859, 1047, 2090
BstFNI CGCG 2 cut(s) 322, 2668
BstH2I RGCGCY 1 cut(s) 1084
BstHHI GCGC 1 cut(s) 1083
BstMAI GTCTC 2 cut(s) 73, 715
BstMWI GCNNNNNNNGC 9 cut(s) 53, 83, 103, 440, 1087, 1151, 2326, 2476, 2538
BstNSI RCATGY 7 cut(s) 1003, 1039, 1168, 1222, 1392, 1601, 1764
BstPAI GACNNNNGTC 1 cut(s) 1600
BstSFI CTRYAG 3 cut(s) 1155, 2343, 2354
BstSLI GKGCMC 3 cut(s) 88, 474, 1639
BstSNI TACGTA 2 cut(s) 1432, 1493
BstUI CGCG 2 cut(s) 322, 2668
BstV1I GCAGC 6 cut(s) 243, 745, 1090, 1163, 1902, 2525
BstV2I GAAGAC 3 cut(s) 1763, 2270, 2520
BstX2I RGATCY 3 cut(s) 196, 793, 1451
BstYI RGATCY 3 cut(s) 196, 793, 1451
Bsu15I ATCGAT 1 cut(s) 2374
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 2 cut(s) 1731, 2642
BsuTUI ATCGAT 1 cut(s) 2374
BtsCI GGATG 6 cut(s) 151, 492, 787, 859, 1047, 2090
BtsIMutI CAGTG 4 cut(s) 289, 570, 589, 2331
BveI ACCTGC 1 cut(s) 1258
Cac8I GCNNGC 4 cut(s) 45, 441, 1166, 2177
CciI TCATGA 1 cut(s) 1516
CfoI GCGC 1 cut(s) 1083
Cfr13I GGNCC 2 cut(s) 767, 2267
ClaI ATCGAT 1 cut(s) 2374
CseI GACGC 4 cut(s) 515, 697, 2058, 2612
Csp6I GTAC 6 cut(s) 674, 1429, 1549, 1930, 2506, 2659
CspCI CAANNNNNGTGG 5 cut(s) 36, 1013, 1048, 1269, 1304
CviQI GTAC 6 cut(s) 674, 1429, 1549, 1930, 2506, 2659
DdeI CTNAG 4 cut(s) 435, 712, 824, 2252
DraI TTTAAA 3 cut(s) 909, 1011, 1063
EaeI YGGCCR 2 cut(s) 1729, 2640
Eam1104I CTCTTC 3 cut(s) 520, 1689, 2106
EarI CTCTTC 3 cut(s) 520, 1689, 2106
Ecl136II GAGCTC 1 cut(s) 97
Eco105I TACGTA 2 cut(s) 1432, 1493
Eco130I CCWWGG 2 cut(s) 72, 2324
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 73
Eco32I GATATC 1 cut(s) 1368
Eco47I GGWCC 2 cut(s) 767, 2267
Eco47III AGCGCT 1 cut(s) 1082
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 5 cut(s) 125, 1713, 1743, 2225, 2355
Eco72I CACGTG 1 cut(s) 2587
Eco88I CYCGRG 1 cut(s) 2423
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 2267
EcoRI GAATTC 2 cut(s) 2120, 2299
EcoRV GATATC 1 cut(s) 1368
EcoT14I CCWWGG 2 cut(s) 72, 2324
EcoT22I ATGCAT 3 cut(s) 1005, 1276, 1766
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 2 cut(s) 72, 2324
Esp3I CGTCTC 1 cut(s) 715
FalI AAGNNNNNCTT 8 cut(s) 545, 577, 807, 839, 1365, 1397, 1608, 1640
FaqI GGGAC 3 cut(s) 369, 1554, 2592
FauI CCCGC 1 cut(s) 2673
FbaI TGATCA 1 cut(s) 1989
Fnu4HI GCNGC 7 cut(s) 257, 759, 1079, 1152, 1916, 2468, 2539
FokI GGATG 6 cut(s) 158, 499, 794, 866, 1034, 2097
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 7 cut(s) 257, 759, 1079, 1152, 1916, 2468, 2539
GlaI GCGC 1 cut(s) 1082
GluI GCNGC 7 cut(s) 257, 759, 1079, 1152, 1916, 2468, 2539
GsuI CTGGAG 2 cut(s) 565, 1820
HaeII RGCGCY 1 cut(s) 1084
HaeIII GGCC 2 cut(s) 1731, 2642
HapII CCGG 1 cut(s) 2399
HgaI GACGC 4 cut(s) 515, 697, 2058, 2612
HhaI GCGC 1 cut(s) 1083
Hin1I GRCGYC 3 cut(s) 2050, 2581, 2674
Hin6I GCGC 1 cut(s) 1081
HinP1I GCGC 1 cut(s) 1081
HincII GTYRAC 2 cut(s) 277, 505
HindII GTYRAC 2 cut(s) 277, 505
HindIII AAGCTT 2 cut(s) 911, 2237
HinfI GANTC 8 cut(s) 169, 291, 328, 968, 1773, 1857, 2210, 2339
HpaII CCGG 1 cut(s) 2399
HphI GGTGA 3 cut(s) 91, 2047, 2570
Hpy166II GTNNAC 5 cut(s) 277, 472, 505, 1313, 1346
Hpy188I TCNGA 2 cut(s) 715, 1208
Hpy8I GTNNAC 5 cut(s) 277, 472, 505, 1313, 1346
Hpy99I CGWCG 3 cut(s) 2648, 2673, 2676
HpyAV CCTTC 4 cut(s) 1625, 2033, 2144, 2258
HpyCH4III ACNGT 6 cut(s) 284, 1343, 1558, 2505, 2519, 2648
HpyCH4IV ACGT 8 cut(s) 30, 1189, 1431, 1492, 1744, 2027, 2581, 2586
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 83, 103, 440, 1087, 1151, 2326, 2476, 2538
HpyF3I CTNAG 4 cut(s) 435, 712, 824, 2252
HpySE526I ACGT 8 cut(s) 30, 1189, 1431, 1492, 1744, 2027, 2581, 2586
Hsp92I GRCGYC 3 cut(s) 2050, 2581, 2674
HspAI GCGC 1 cut(s) 1081
Kpn2I TCCGGA 1 cut(s) 2398
KpnI GGTACC 1 cut(s) 2662
Ksp22I TGATCA 1 cut(s) 1989
LmnI GCTCC 4 cut(s) 500, 929, 1075, 2453
Lsp1109I GCAGC 6 cut(s) 243, 745, 1090, 1163, 1902, 2525
LweI GCATC 5 cut(s) 136, 565, 1261, 1773, 2238
MaeII ACGT 8 cut(s) 30, 1189, 1431, 1492, 1744, 2027, 2581, 2586
MaeIII GTNAC 3 cut(s) 883, 2582, 2587
MflI RGATCY 3 cut(s) 196, 793, 1451
MhlI GDGCHC 4 cut(s) 88, 99, 474, 1639
MlsI TGGCCA 1 cut(s) 1731
MluNI TGGCCA 1 cut(s) 1731
MlyI GAGTC 1 cut(s) 300
MmeI TCCRAC 2 cut(s) 305, 831
Mox20I TGGCCA 1 cut(s) 1731
Mph1103I ATGCAT 3 cut(s) 1005, 1276, 1766
MroI TCCGGA 1 cut(s) 2398
MroXI GAANNNNTTC 1 cut(s) 1590
MscI TGGCCA 1 cut(s) 1731
Msp20I TGGCCA 1 cut(s) 1731
MspI CCGG 1 cut(s) 2399
Mva1269I GAATGC 3 cut(s) 1003, 2177, 2450
MvnI CGCG 2 cut(s) 322, 2668
MwoI GCNNNNNNNGC 9 cut(s) 53, 83, 103, 440, 1087, 1151, 2326, 2476, 2538
NheI GCTAGC 1 cut(s) 43
NlaIV GGNNCC 4 cut(s) 85, 1356, 1985, 2660
NmuCI GTSAC 2 cut(s) 2582, 2587
NsiI ATGCAT 3 cut(s) 1005, 1276, 1766
NspI RCATGY 7 cut(s) 1003, 1039, 1168, 1222, 1392, 1601, 1764
PaeI GCATGC 1 cut(s) 1168
PaeR7I CTCGAG 1 cut(s) 2423
PagI TCATGA 1 cut(s) 1516
PciI ACATGT 2 cut(s) 1218, 1597
PctI GAATGC 3 cut(s) 1003, 2177, 2450
PdmI GAANNNNTTC 1 cut(s) 1590
PfeI GAWTC 7 cut(s) 169, 328, 968, 1773, 1857, 2210, 2339
PflFI GACNNNGTC 1 cut(s) 227
PkrI GCNGC 7 cut(s) 258, 760, 1080, 1153, 1917, 2469, 2540
PleI GAGTC 1 cut(s) 299
PmaCI CACGTG 1 cut(s) 2587
PmlI CACGTG 1 cut(s) 2587
PpsI GAGTC 1 cut(s) 299
Ppu21I YACGTR 3 cut(s) 1432, 1493, 2587
PpuMI RGGWCCY 1 cut(s) 2267
PscI ACATGT 2 cut(s) 1218, 1597
PshAI GACNNNNGTC 1 cut(s) 1600
PshBI ATTAAT 1 cut(s) 737
Psp124BI GAGCTC 1 cut(s) 99
Psp1406I AACGTT 1 cut(s) 1744
Psp5II RGGWCCY 1 cut(s) 2267
PspCI CACGTG 1 cut(s) 2587
PspN4I GGNNCC 4 cut(s) 85, 1356, 1985, 2660
PspPI GGNCC 2 cut(s) 767, 2267
PspPPI RGGWCCY 1 cut(s) 2267
PspXI VCTCGAGB 1 cut(s) 2423
PstI CTGCAG 1 cut(s) 2347
PsuI RGATCY 3 cut(s) 196, 793, 1451
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 6 cut(s) 675, 1430, 1550, 1931, 2507, 2660
RsaNI GTAC 6 cut(s) 674, 1429, 1549, 1930, 2506, 2659
SacI GAGCTC 1 cut(s) 99
SatI GCNGC 7 cut(s) 257, 759, 1079, 1152, 1916, 2468, 2539
Sau96I GGNCC 2 cut(s) 767, 2267
ScaI AGTACT 2 cut(s) 1550, 1931
SchI GAGTC 1 cut(s) 300
SduI GDGCHC 4 cut(s) 88, 99, 474, 1639
SfaNI GCATC 5 cut(s) 136, 565, 1261, 1773, 2238
SfcI CTRYAG 3 cut(s) 1155, 2343, 2354
Sfr274I CTCGAG 1 cut(s) 2423
SinI GGWCC 2 cut(s) 767, 2267
SlaI CTCGAG 1 cut(s) 2423
SmlI CTYRAG 5 cut(s) 92, 1658, 1768, 1958, 2423
SmoI CTYRAG 5 cut(s) 92, 1658, 1768, 1958, 2423
SnaBI TACGTA 2 cut(s) 1432, 1493
SpeI ACTAGT 2 cut(s) 133, 385
SphI GCATGC 1 cut(s) 1168
SsiI CCGC 5 cut(s) 259, 1786, 2202, 2468, 2666
SspI AATATT 2 cut(s) 1243, 1608
SstI GAGCTC 1 cut(s) 99
StyI CCWWGG 2 cut(s) 72, 2324
TaaI ACNGT 6 cut(s) 284, 1343, 1558, 2505, 2519, 2648
TaiI ACGT 8 cut(s) 33, 1192, 1434, 1495, 1747, 2030, 2584, 2589
TatI WGTACW 3 cut(s) 1548, 1929, 2505
TauI GCSGC 1 cut(s) 2470
TfiI GAWTC 7 cut(s) 169, 328, 968, 1773, 1857, 2210, 2339
TscAI CASTG 4 cut(s) 289, 577, 589, 2338
TseFI GTSAC 2 cut(s) 2582, 2587
TseI GCWGC 6 cut(s) 256, 758, 1078, 1151, 1915, 2538
Tsp45I GTSAC 2 cut(s) 2582, 2587
TspRI CASTG 4 cut(s) 289, 577, 589, 2338
Tth111I GACNNNGTC 1 cut(s) 227
VneI GTGCAC 1 cut(s) 470
VpaK11BI GGWCC 2 cut(s) 767, 2267
VspI ATTAAT 1 cut(s) 737
XapI RAATTY 6 cut(s) 682, 837, 1921, 2120, 2299, 2417
XbaI TCTAGA 1 cut(s) 1448
XceI RCATGY 7 cut(s) 1003, 1039, 1168, 1222, 1392, 1601, 1764
XhoI CTCGAG 1 cut(s) 2423
XmnI GAANNNNTTC 1 cut(s) 1590
ZraI GACGTC 1 cut(s) 2582
ZrmI AGTACT 2 cut(s) 1550, 1931
Zsp2I ATGCAT 3 cut(s) 1005, 1276, 1766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.