Rw7G002510

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000159
Physical Location & Seq
Forward (+)
2066832 .. 2069809
2978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002510.1

Sequence Viewer

Length: 2715 bp
ATGGAGTTTGCCTCTGGCATTGCTGAGACAGTCTTGGTTAGGCTAGCCTCCTATGCTGCCCAAGAAGTTTGCTTGGCTTGGGGTGCCCAACTTGAGCTCAAGAAGCTCAATAAGACCTTGTCAGCCATCAAACTTGTACTCCAGGATGCAGAGAGGAAGCAAATGCAGAACCCCCTCATCACTCATTGGCTAGGAAACCTTAAAGATGTTTGCAACGATGCTGAGGATGTACTCGACGAATTTGAGTTCCGAAAGCTCCGACTCGAAGTGCTCACCAACGACCACCGGAGTGTCAAAGCAAAGGTACACCAATTCTTCTCCCGTTGGAATCCAGTCGTGTTTAACTTTAAAATGGGTCATAAAATCAAAGAGATTAGAGAAAGACTAGATGAGATTGATGCTGAAAAGACTAACTTTTCTCTCATTCAAGTTGTTGAACTAGTTGATGATTGTGGTGAGCCCCAAAGGGCACATGTTAATAAAAGGGAGACAGACTCTTTACTGGAAGGTCATGTTGTTGGAAGAGATGATGATAAGGAAAACATTATCCTTCTCTTTGATGACACCCGTTCCTCCAGTGTCGATAATGTTTCAGTCATTTCCATCTTTGGGTTAGCAGGGTTGGGAAAAACCACACTCGCTAAATCAGTGTATAATGATACTGCGGTGGAGAATAAATTTGATATTAGAATGTGGGTATGCGTCTCAGAAAACTTTGATATCCATACATTAGTTCGTGGCATTATTGCTGCAACTAAACAAGAATGTGCCGGTGAAAGTTTAGATCTTATGAAAAGGGGGTTGCAAGATATTTTGAGAGATAAAAAGTTTTTGTTAGTGTTGGACGATGTATGGGATAAAGAGTCGACGGGAGTTACACATCAAAAATGGAGTGAGTTAAAAACTGTGTTACATGTTGGAGCCAAAGGGAGTAAAATCATCGTAACAACCCGTAATGAATCAGTCGCTTCACTTGTAAACCCTGTACATATGCATTCTTTAGAAGGTCTTTCTCATGAGGACTCCATGACTTTGTTTAAAAAAAATGCATTTAACGAAGGAGAGGAGTCACACTATCAACACTTGATAAAAATTGGAGAGGACATTGTGAAGAAGTGTGGAGGGGTTCCCTTAGCACTAGCTACTCTAGGGAGTTTACTTCACTCGAAAAGGGAGCGACGTCAGTGGTTACATGTAAGAGATAATGAAATATGGAGTTTGCCCGGAAATGACAACATTATAGCTGCACTAAAATTGAGCTACAATGCACTGCCACCTCACTTGAAGCCTTGCTTTGCCTTTTGTTCACTTTACCCCAAGGATTATGAATTTGCAAGTGAAGAAATTGTCCAATTATGGATGGCACAAGGCTTCCTCAAGTCCTCAAGAGAAAATGAAGATTTTGACGAGATGGGCCTAGACTATATTAGGCAGTTCTGTTCCAAGTCTTTATTTCAACTTGACGTTGATGTGAAGACTGCTGTGCAGTTCAAAATCCATGATTTAGTGCATGACTTGGCAATATCAGTGGCACGGGCAGATTGCTCCTCAATTAATTTCCGTCCCTCTAGTGCTTTTGAAAAAGTCCGACACGTGTCAATATCGGAAAAAGACTTGTCTGGGGATGTGGCAGGAGTCCCGGCATTCATTCTCCAGTCGGAGAAACTGCGGACCATTCTAAATCTAGATAGTGAAGCTGGGATCTCCAATCAATATTTTCTAAAGACATGCATCTTGAGATTCAAGTATCTGCGTGCGCTAGATGTGCGTGGTTCTACTCTTGAGGAGCTACCAGGTTCCATTGGTAACTTGATTCATTTAAGATATCTCAACCTGGGCGGAAATGCAAAGATAAAACAGCTTCCCAGTTCCATTTGCAAGCTCTTGAATTTGCAGTTCTTAAGCCTTGCCCGTTGTGAGGCACTTGAGGAGTTACCCAAAGGCATAGGGAACCTGATCAATCTCAGATATTTGAATATAACTACACAGCAGATGTATTTTCCGAAAGGAGTATTTCGACGCCTCACCTTACTTCAATCCTTGATCATTGGGTATTGTGCGAATCTGAAATCTCTGGGAGAAGAGATCCAATACCTCACTAACCTTCGTCAACTGTGGATCCGTTACTGTGAAAATCTGGAGTCCTTGCCACCTAACATGAAGAACTTGACTGCATTCCATACTTTGGCGATCGTTGATTGTAAGAAGCTTGAGTTAATGAGGTCGGGAGAAGGCATCAATGGTCTCAGGTCATTCGCTGTCCTCACATCAGCTTTGGAGGCTTTGCCCAATTGGCTTCAAGAGTCTGCAGTCACTTTACAGAACATGACAATCATAGAGTGGGATAATCTCGCGGCACTTCCAGAGTGGTTACAAAATCTCACCTTGCTGGAGCAACTGGCGATTGGGAAATGCCCCAAATTGTCTGCTTTGCCGCAAGGGATGCATCGCCTCACTGCGCTGAGAGAATTGCGGATTGGCGGGTGTCCTGAATTGAGAAAAAGATGCAAAAGGGAAACAGGAGAGGATTGGTCCAAGATCAAACATGTCCAAAAGATTGAACTCGATGACGAGGTGCGGATAATATCGATGATTAGGAAACAAATTACTTGTTCCACTACTGTGATGCTTTTCATAAATTGTTTGCACTTCTTATCCCATAAGCTCTTTATTATTTGTCTTAGATTTAATACATTCTATCCGTCAATTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

904

Amino Acids

102.66

Weight (kDa)

6.96

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 94 7.7e-21 Rx N-terminal domain
NB-ARC PF00931 195 - 353 5.7e-35 NB-ARC domain
WHD_DRP PF23559 437 - 507 8.3e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 578 - 777 3.5e-26 Leucine-rich repeat region
LRR_13 PF23286 592 - 739 2e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1183
AccB1I GGYRCC 1 cut(s) 83
AccB7I CCANNNNNTGG 1 cut(s) 2185
AccI GTMKAC 1 cut(s) 866
AccII CGCG 1 cut(s) 2354
AciI CCGC 8 cut(s) 665, 1669, 1839, 2354, 2435, 2473, 2481, 2578
AclWI GGATC 4 cut(s) 1709, 2080, 2113, 2126
AcsI RAATTY 4 cut(s) 239, 677, 1328, 1888
AcvI CACGTG 1 cut(s) 1594
AcyI GRCGYC 2 cut(s) 1180, 2020
AfaI GTAC 4 cut(s) 138, 231, 306, 987
AfiI CCNNNNNNNGG 3 cut(s) 609, 1918, 2185
AflII CTTAAG 1 cut(s) 1900
AflIII ACRYGT 6 cut(s) 472, 913, 1192, 1591, 1593, 2545
AhlI ACTAGT 1 cut(s) 439
AjnI CCWGG 3 cut(s) 141, 1792, 1833
AjuI GAANNNNNNNTTGG 2 cut(s) 2460, 2492
AleI CACNNNNGTG 2 cut(s) 288, 2621
Alw21I GWGCWC 2 cut(s) 99, 273
Alw26I GTCTC 4 cut(s) 20, 482, 709, 2249
AlwI GGATC 4 cut(s) 1709, 2080, 2113, 2126
AoxI GGCC 1 cut(s) 1414
ApeKI GCWGC 3 cut(s) 56, 749, 1244
ApoI RAATTY 4 cut(s) 239, 677, 1328, 1888
AseI ATTAAT 1 cut(s) 1554
AspLEI GCGC 2 cut(s) 1759, 2461
AspS9I GGNCC 3 cut(s) 1414, 1671, 2532
AsuC2I CCSGG 2 cut(s) 1224, 1640
AsuHPI GGTGA 5 cut(s) 265, 467, 785, 2017, 2374
AsuNHI GCTAGC 1 cut(s) 43
AvaII GGWCC 2 cut(s) 1671, 2532
BaeGI GKGCMC 2 cut(s) 88, 472
BaeI ACNNNNGTAYC 2 cut(s) 651, 684
BamHI GGATCC 1 cut(s) 2118
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 2 cut(s) 99, 462
BbrPI CACGTG 1 cut(s) 1594
BbsI GAAGAC 1 cut(s) 1481
Bbv12I GWGCWC 2 cut(s) 99, 273
BbvCI CCTCAGC 1 cut(s) 222
BbvI GCAGC 3 cut(s) 43, 736, 1231
BccI CCATC 4 cut(s) 134, 611, 1354, 1405
BciT130I CCWGG 3 cut(s) 143, 1794, 1835
BclI TGATCA 2 cut(s) 1956, 2043
BcnI CCSGG 2 cut(s) 1224, 1640
BcoDI GTCTC 4 cut(s) 20, 482, 709, 2249
BcuI ACTAGT 1 cut(s) 439
BfmI CTRYAG 1 cut(s) 2307
BfrI CTTAAG 1 cut(s) 1900
BglI GCCNNNNNGGC 1 cut(s) 2293
BglII AGATCT 1 cut(s) 784
BisI GCNGC 5 cut(s) 57, 750, 1245, 2355, 2435
BlsI GCNGC 5 cut(s) 58, 751, 1246, 2356, 2436
Bme1390I CCNGG 5 cut(s) 143, 1224, 1640, 1794, 1835
Bme18I GGWCC 2 cut(s) 1671, 2532
BmgT120I GGNCC 3 cut(s) 1414, 1671, 2532
BmiI GGNNCC 6 cut(s) 85, 922, 1128, 1798, 1952, 2120
BmrFI CCNGG 5 cut(s) 143, 1224, 1640, 1794, 1835
BmrI ACTGGG 1 cut(s) 1860
BmsI GCATC 9 cut(s) 136, 208, 388, 1740, 2244, 2433, 2455, 2495, 2616
BmtI GCTAGC 1 cut(s) 47
BmuI ACTGGG 1 cut(s) 1860
BoxI GACNNNNGTC 1 cut(s) 1594
BpiI GAAGAC 1 cut(s) 1481
BplI GAGNNNNNCTC 3 cut(s) 28, 479, 511
BpmI CTGGAG 5 cut(s) 125, 559, 1637, 2159, 2411
Bpu10I CCTNAGC 2 cut(s) 222, 1132
BpuEI CTTGAG 8 cut(s) 83, 113, 1361, 1369, 1756, 1802, 1946, 2231
BpuMI CCSGG 2 cut(s) 1224, 1640
Bsa29I ATCGAT 1 cut(s) 2588
BsaAI YACGTR 1 cut(s) 1594
BsaHI GRCGYC 2 cut(s) 1180, 2020
BsaI GGTCTC 1 cut(s) 2249
BsaJI CCNNGG 2 cut(s) 1317, 1834
BsaWI WCCGGW 1 cut(s) 285
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bsc4I CCNNNNNNNGG 3 cut(s) 609, 1918, 2185
Bse118I RCCGGY 1 cut(s) 770
Bse1I ACTGG 6 cut(s) 332, 507, 576, 1654, 1866, 2403
Bse3DI GCAATG 1 cut(s) 18
BseBI CCWGG 3 cut(s) 143, 1794, 1835
BseCI ATCGAT 1 cut(s) 2588
BseDI CCNNGG 2 cut(s) 1317, 1834
BseGI GGATG 5 cut(s) 151, 232, 1365, 1630, 2448
BseLI CCNNNNNNNGG 3 cut(s) 609, 1918, 2185
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 6 cut(s) 15, 213, 720, 1978, 2260, 2453
BseNI ACTGG 6 cut(s) 332, 507, 576, 1654, 1866, 2403
BseRI GAGGAG 4 cut(s) 1079, 1537, 1799, 1943
BseSI GKGCMC 2 cut(s) 88, 472
BseXI GCAGC 3 cut(s) 43, 736, 1231
BseYI CCCAGC 1 cut(s) 1697
BsgI GTGCAG 2 cut(s) 1230, 1505
Bsh1236I CGCG 1 cut(s) 2354
Bsh1285I CGRYCG 1 cut(s) 2193
BshFI GGCC 1 cut(s) 1416
BshNI GGYRCC 1 cut(s) 83
BshVI ATCGAT 1 cut(s) 2588
BsiEI CGRYCG 1 cut(s) 2193
BsiHKAI GWGCWC 2 cut(s) 99, 273
BsiSI CCGG 4 cut(s) 286, 771, 1224, 1640
BslFI GGGAC 2 cut(s) 1548, 1622
BslI CCNNNNNNNGG 3 cut(s) 609, 1918, 2185
BsmAI GTCTC 4 cut(s) 20, 482, 709, 2249
BsmBI CGTCTC 1 cut(s) 709
BsmFI GGGAC 2 cut(s) 1548, 1622
BsmI GAATGC 3 cut(s) 994, 1643, 2174
BsnI GGCC 1 cut(s) 1416
Bso31I GGTCTC 1 cut(s) 2249
Bsp1286I GDGCHC 5 cut(s) 88, 99, 273, 462, 472
Bsp1407I TGTACA 1 cut(s) 985
Bsp143I GATC 8 cut(s) 784, 1701, 1956, 2043, 2085, 2118, 2190, 2538
BspACI CCGC 8 cut(s) 665, 1669, 1839, 2354, 2435, 2473, 2481, 2578
BspANI GGCC 1 cut(s) 1416
BspCNI CTCAG 6 cut(s) 16, 214, 719, 1977, 2259, 2454
BspDI ATCGAT 1 cut(s) 2588
BspFNI CGCG 1 cut(s) 2354
BspHI TCATGA 1 cut(s) 1015
BspLI GGNNCC 6 cut(s) 85, 922, 1128, 1798, 1952, 2120
BspMAI CTGCAG 1 cut(s) 2311
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 4 cut(s) 1709, 2080, 2113, 2126
BspT107I GGYRCC 1 cut(s) 83
BspTI CTTAAG 1 cut(s) 1900
BspTNI GGTCTC 1 cut(s) 2249
BsrDI GCAATG 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 770
BsrGI TGTACA 1 cut(s) 985
BsrI ACTGG 6 cut(s) 332, 507, 576, 1654, 1866, 2403
BssAI RCCGGY 1 cut(s) 770
BssECI CCNNGG 2 cut(s) 1317, 1834
BssMI GATC 8 cut(s) 784, 1701, 1956, 2043, 2085, 2118, 2190, 2538
BssNI GRCGYC 2 cut(s) 1180, 2020
BssT1I CCWWGG 1 cut(s) 1317
Bst2UI CCWGG 3 cut(s) 143, 1794, 1835
Bst4CI ACNGT 5 cut(s) 31, 907, 2115, 2129, 2623
Bst6I CTCTTC 2 cut(s) 517, 2076
BstACI GRCGYC 2 cut(s) 1180, 2020
BstAFI CTTAAG 1 cut(s) 1900
BstAPI GCANNNNNTGC 1 cut(s) 2443
BstAUI TGTACA 1 cut(s) 985
BstBAI YACGTR 1 cut(s) 1594
BstC8I GCNNGC 3 cut(s) 45, 1755, 1880
BstDEI CTNAG 8 cut(s) 24, 222, 706, 1132, 1964, 2246, 2462, 2681
BstF5I GGATG 5 cut(s) 151, 232, 1365, 1630, 2448
BstFNI CGCG 1 cut(s) 2354
BstHHI GCGC 2 cut(s) 1759, 2461
BstKTI GATC 8 cut(s) 787, 1704, 1959, 2046, 2088, 2121, 2193, 2541
BstMAI GTCTC 4 cut(s) 20, 482, 709, 2249
BstMBI GATC 8 cut(s) 784, 1701, 1956, 2043, 2085, 2118, 2190, 2538
BstMCI CGRYCG 1 cut(s) 2193
BstMWI GCNNNNNNNGC 7 cut(s) 53, 83, 103, 1765, 2279, 2293, 2443
BstNI CCWGG 3 cut(s) 143, 1794, 1835
BstNSI RCATGY 5 cut(s) 476, 917, 1196, 1731, 2549
BstPAI GACNNNNGTC 1 cut(s) 1594
BstSCI CCNGG 5 cut(s) 141, 1222, 1638, 1792, 1833
BstSFI CTRYAG 1 cut(s) 2307
BstSLI GKGCMC 2 cut(s) 88, 472
BstUI CGCG 1 cut(s) 2354
BstV1I GCAGC 3 cut(s) 43, 736, 1231
BstV2I GAAGAC 1 cut(s) 1481
BstX2I RGATCY 4 cut(s) 784, 1701, 2085, 2118
BstYI RGATCY 4 cut(s) 784, 1701, 2085, 2118
Bsu15I ATCGAT 1 cut(s) 2588
BsuRI GGCC 1 cut(s) 1416
BsuTUI ATCGAT 1 cut(s) 2588
BtgZI GCGATG 1 cut(s) 2432
BtsCI GGATG 5 cut(s) 151, 232, 1365, 1630, 2448
BtsI GCAGTG 2 cut(s) 1268, 2454
BtsIMutI CAGTG 6 cut(s) 583, 654, 1190, 1268, 1533, 2454
Cac8I GCNNGC 3 cut(s) 45, 1755, 1880
CciI TCATGA 1 cut(s) 1015
CfoI GCGC 2 cut(s) 1759, 2461
Cfr10I RCCGGY 1 cut(s) 770
Cfr13I GGNCC 3 cut(s) 1414, 1671, 2532
ClaI ATCGAT 1 cut(s) 2588
CseI GACGC 2 cut(s) 691, 2028
CsiI ACCWGGT 1 cut(s) 1792
Csp6I GTAC 4 cut(s) 137, 230, 305, 986
CspCI CAANNNNNGTGG 4 cut(s) 1263, 1298, 1509, 1544
CviQI GTAC 4 cut(s) 137, 230, 305, 986
DdeI CTNAG 8 cut(s) 24, 222, 706, 1132, 1964, 2246, 2462, 2681
DpnI GATC 8 cut(s) 786, 1703, 1958, 2045, 2087, 2120, 2192, 2540
DpnII GATC 8 cut(s) 784, 1701, 1956, 2043, 2085, 2118, 2190, 2538
DraI TTTAAA 2 cut(s) 349, 1039
Eam1104I CTCTTC 2 cut(s) 517, 2076
EarI CTCTTC 2 cut(s) 517, 2076
EciI GGCGGA 1 cut(s) 1854
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 1317
Eco24I GRGCYC 2 cut(s) 99, 462
Eco31I GGTCTC 1 cut(s) 2249
Eco32I GATATC 2 cut(s) 721, 1826
Eco47I GGWCC 2 cut(s) 1671, 2532
Eco53kI GAGCTC 1 cut(s) 97
Eco72I CACGTG 1 cut(s) 1594
EcoICRI GAGCTC 1 cut(s) 97
EcoRII CCWGG 3 cut(s) 141, 1792, 1833
EcoRV GATATC 2 cut(s) 721, 1826
EcoT14I CCWWGG 1 cut(s) 1317
EcoT22I ATGCAT 4 cut(s) 996, 1051, 1733, 2448
EcoT38I GRGCYC 2 cut(s) 99, 462
ErhI CCWWGG 1 cut(s) 1317
Esp3I CGTCTC 1 cut(s) 709
FalI AAGNNNNNCTT 4 cut(s) 398, 430, 1277, 1309
FaqI GGGAC 2 cut(s) 1548, 1622
FauI CCCGC 1 cut(s) 2474
FauNDI CATATG 1 cut(s) 990
FbaI TGATCA 2 cut(s) 1956, 2043
FblI GTMKAC 1 cut(s) 866
Fnu4HI GCNGC 5 cut(s) 57, 750, 1245, 2355, 2435
FokI GGATG 5 cut(s) 158, 239, 1372, 1637, 2455
FriOI GRGCYC 2 cut(s) 99, 462
Fsp4HI GCNGC 5 cut(s) 57, 750, 1245, 2355, 2435
GlaI GCGC 2 cut(s) 1758, 2460
GluI GCNGC 5 cut(s) 57, 750, 1245, 2355, 2435
GsaI CCCAGC 1 cut(s) 1701
GsuI CTGGAG 5 cut(s) 125, 559, 1637, 2159, 2411
HaeIII GGCC 1 cut(s) 1416
HapII CCGG 4 cut(s) 286, 771, 1224, 1640
HgaI GACGC 2 cut(s) 691, 2028
HhaI GCGC 2 cut(s) 1759, 2461
Hin1I GRCGYC 2 cut(s) 1180, 2020
Hin6I GCGC 2 cut(s) 1757, 2459
HinP1I GCGC 2 cut(s) 1757, 2459
HincII GTYRAC 2 cut(s) 867, 2111
HindII GTYRAC 2 cut(s) 867, 2111
HindIII AAGCTT 1 cut(s) 2207
HpaII CCGG 4 cut(s) 286, 771, 1224, 1640
HphI GGTGA 5 cut(s) 265, 467, 785, 2017, 2374
Hpy166II GTNNAC 6 cut(s) 307, 867, 979, 1157, 1307, 2111
Hpy188I TCNGA 9 cut(s) 251, 260, 709, 1589, 1606, 1660, 1967, 2004, 2067
Hpy8I GTNNAC 6 cut(s) 307, 867, 979, 1157, 1307, 2111
Hpy99I CGWCG 4 cut(s) 239, 871, 1182, 2022
HpyAV CCTTC 6 cut(s) 500, 560, 998, 1052, 2114, 2225
HpyCH4III ACNGT 5 cut(s) 31, 907, 2115, 2129, 2623
HpyCH4IV ACGT 3 cut(s) 1180, 1464, 1593
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 83, 103, 1765, 2279, 2293, 2443
HpyF3I CTNAG 8 cut(s) 24, 222, 706, 1132, 1964, 2246, 2462, 2681
HpySE526I ACGT 3 cut(s) 1180, 1464, 1593
Hsp92I GRCGYC 2 cut(s) 1180, 2020
HspAI GCGC 2 cut(s) 1757, 2459
Ksp22I TGATCA 2 cut(s) 1956, 2043
Kzo9I GATC 8 cut(s) 784, 1701, 1956, 2043, 2085, 2118, 2190, 2538
LmnI GCTCC 6 cut(s) 261, 920, 1174, 1550, 1786, 2392
Lsp1109I GCAGC 3 cut(s) 43, 736, 1231
LweI GCATC 9 cut(s) 136, 208, 388, 1740, 2244, 2433, 2455, 2495, 2616
MabI ACCWGGT 1 cut(s) 1792
MaeII ACGT 3 cut(s) 1180, 1464, 1593
MalI GATC 8 cut(s) 786, 1703, 1958, 2045, 2087, 2120, 2192, 2540
MboI GATC 8 cut(s) 784, 1701, 1956, 2043, 2085, 2118, 2190, 2538
MboII GAAGA 8 cut(s) 307, 534, 1123, 1352, 1409, 1486, 2093, 2173
MfeI CAATTG 1 cut(s) 2290
MflI RGATCY 4 cut(s) 784, 1701, 2085, 2118
MhlI GDGCHC 5 cut(s) 88, 99, 273, 462, 472
MlyI GAGTC 8 cut(s) 255, 488, 872, 1016, 1076, 1644, 2150, 2312
MmeI TCCRAC 7 cut(s) 283, 305, 499, 822, 898, 1612, 1638
Mph1103I ATGCAT 4 cut(s) 996, 1051, 1733, 2448
MslI CAYNNNNRTG 2 cut(s) 288, 2621
MspCI CTTAAG 1 cut(s) 1900
MspI CCGG 4 cut(s) 286, 771, 1224, 1640
MspR9I CCNGG 5 cut(s) 143, 1224, 1640, 1794, 1835
MunI CAATTG 1 cut(s) 2290
Mva1269I GAATGC 3 cut(s) 994, 1643, 2174
MvaI CCWGG 3 cut(s) 143, 1794, 1835
MvnI CGCG 1 cut(s) 2354
MwoI GCNNNNNNNGC 7 cut(s) 53, 83, 103, 1765, 2279, 2293, 2443
NciI CCSGG 2 cut(s) 1224, 1640
NdeI CATATG 1 cut(s) 990
NdeII GATC 8 cut(s) 784, 1701, 1956, 2043, 2085, 2118, 2190, 2538
NheI GCTAGC 1 cut(s) 43
NlaIV GGNNCC 6 cut(s) 85, 922, 1128, 1798, 1952, 2120
NmuCI GTSAC 2 cut(s) 1068, 2311
NsiI ATGCAT 4 cut(s) 996, 1051, 1733, 2448
NspI RCATGY 5 cut(s) 476, 917, 1196, 1731, 2549
OliI CACNNNNGTG 2 cut(s) 288, 2621
PagI TCATGA 1 cut(s) 1015
PciI ACATGT 4 cut(s) 472, 913, 1192, 2545
PctI GAATGC 3 cut(s) 994, 1643, 2174
PfeI GAWTC 5 cut(s) 328, 959, 1740, 1813, 2062
PflFI GACNNNGTC 1 cut(s) 118
PflMI CCANNNNNTGG 1 cut(s) 2185
PfoI TCCNGGA 1 cut(s) 141
PkrI GCNGC 5 cut(s) 58, 751, 1246, 2356, 2436
Ple19I CGATCG 1 cut(s) 2193
PleI GAGTC 8 cut(s) 255, 488, 871, 1016, 1075, 1643, 2149, 2311
PmaCI CACGTG 1 cut(s) 1594
PmlI CACGTG 1 cut(s) 1594
PpsI GAGTC 8 cut(s) 255, 488, 871, 1016, 1075, 1643, 2149, 2311
Ppu21I YACGTR 1 cut(s) 1594
PscI ACATGT 4 cut(s) 472, 913, 1192, 2545
PshAI GACNNNNGTC 1 cut(s) 1594
PshBI ATTAAT 1 cut(s) 1554
Psp124BI GAGCTC 1 cut(s) 99
Psp6I CCWGG 3 cut(s) 141, 1792, 1833
PspCI CACGTG 1 cut(s) 1594
PspFI CCCAGC 1 cut(s) 1697
PspGI CCWGG 3 cut(s) 141, 1792, 1833
PspN4I GGNNCC 6 cut(s) 85, 922, 1128, 1798, 1952, 2120
PspPI GGNCC 3 cut(s) 1414, 1671, 2532
PstI CTGCAG 1 cut(s) 2311
PsuI RGATCY 4 cut(s) 784, 1701, 2085, 2118
PsyI GACNNNGTC 1 cut(s) 118
PvuI CGATCG 1 cut(s) 2193
RsaI GTAC 4 cut(s) 138, 231, 306, 987
RsaNI GTAC 4 cut(s) 137, 230, 305, 986
RseI CAYNNNNRTG 2 cut(s) 288, 2621
SacI GAGCTC 1 cut(s) 99
SalI GTCGAC 1 cut(s) 865
SatI GCNGC 5 cut(s) 57, 750, 1245, 2355, 2435
Sau3AI GATC 8 cut(s) 784, 1701, 1956, 2043, 2085, 2118, 2190, 2538
Sau96I GGNCC 3 cut(s) 1414, 1671, 2532
SchI GAGTC 8 cut(s) 255, 488, 872, 1016, 1076, 1644, 2150, 2312
ScrFI CCNGG 5 cut(s) 143, 1224, 1640, 1794, 1835
SduI GDGCHC 5 cut(s) 88, 99, 273, 462, 472
SexAI ACCWGGT 1 cut(s) 1792
SfaNI GCATC 9 cut(s) 136, 208, 388, 1740, 2244, 2433, 2455, 2495, 2616
SfcI CTRYAG 1 cut(s) 2307
SinI GGWCC 2 cut(s) 1671, 2532
SmiMI CAYNNNNRTG 2 cut(s) 288, 2621
SmlI CTYRAG 9 cut(s) 92, 98, 1376, 1384, 1735, 1781, 1900, 1925, 2210
SmoI CTYRAG 9 cut(s) 92, 98, 1376, 1384, 1735, 1781, 1900, 1925, 2210
SpeI ACTAGT 1 cut(s) 439
SsiI CCGC 8 cut(s) 665, 1669, 1839, 2354, 2435, 2473, 2481, 2578
SspI AATATT 1 cut(s) 1715
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 5 cut(s) 141, 1222, 1638, 1792, 1833
StyI CCWWGG 1 cut(s) 1317
TaaI ACNGT 5 cut(s) 31, 907, 2115, 2129, 2623
TaiI ACGT 3 cut(s) 1183, 1467, 1596
TaqI TCGA 8 cut(s) 234, 264, 582, 866, 1166, 2017, 2565, 2588
TatI WGTACW 3 cut(s) 136, 229, 985
TauI GCSGC 2 cut(s) 2357, 2437
TfiI GAWTC 5 cut(s) 328, 959, 1740, 1813, 2062
TscAI CASTG 6 cut(s) 583, 654, 1190, 1275, 1533, 2461
TseFI GTSAC 2 cut(s) 1068, 2311
TseI GCWGC 3 cut(s) 56, 749, 1244
Tsp45I GTSAC 2 cut(s) 1068, 2311
TspDTI ATGAA 9 cut(s) 806, 972, 1221, 1341, 1410, 1636, 1805, 2174, 2623
TspGWI ACGGA 3 cut(s) 1550, 2111, 2691
TspRI CASTG 6 cut(s) 583, 654, 1190, 1275, 1533, 2461
Tth111I GACNNNGTC 1 cut(s) 118
Van91I CCANNNNNTGG 1 cut(s) 2185
Vha464I CTTAAG 1 cut(s) 1900
VpaK11BI GGWCC 2 cut(s) 1671, 2532
VspI ATTAAT 1 cut(s) 1554
XapI RAATTY 4 cut(s) 239, 677, 1328, 1888
XbaI TCTAGA 1 cut(s) 1684
XceI RCATGY 5 cut(s) 476, 917, 1196, 1731, 2549
XmiI GTMKAC 1 cut(s) 866
ZraI GACGTC 1 cut(s) 1181
Zsp2I ATGCAT 4 cut(s) 996, 1051, 1733, 2448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.