Rroxscaffold_4G00321190

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000159
Physical Location & Seq
Reverse (-)
50427769 .. 50436112
8344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00321190.1

Sequence Viewer

Length: 1230 bp
ATGGAGTTTGCCAGCAGCATTGCAGATAACGTCTTGTGTAGGCTAGCTTCACATGCTTCCCAAGAGATCTCTTTGGCATGGGGTGCCCAACTTGAGCTCACCAAGCTCAATAAGACTTTATCTGCCATCAAACTAGTGCTCGAGGATGCTAAGAAGAAGCAAGTGAGAGATCCCCTAATCACTCGTTGGTTGGGAAATCTCAAAGATGTTTGTCATGACGTTGATGACGTCTTGGACGAGCTGGACTTCCAAAAGTTGCGGTTGAAAGTGGAGGTCGACAACTGTGGGAAGATCAAAGGAAAGGTACACCAATTTTTTTCTCAATGGAATCCAGTAGTGTTTAACTTCAAAATGGGATATAAAGTTAAAAAGATTAGAGAAAGACTAGTTGAAATTGATAATGAAAAGAGACAGTTTGCTCTCCTTGATTTTCCTAAGATAGCTAAAGATCCTTGTGCGCCCCAACAAGTGCACGATGACAGGAGGATGACTGCCTCCAAAGTTGAGGCTTCAAATGTTATTGGAAGAGATGATGACAAAAAACAGATTATCAAGCATCTCTTGAATGACACTGATTTCTCCAGTGAGGAGAATGTTTCTGTTGTTTCCATAATTGGGTTAGGAGGGTTGGGGAAAACAACACTTGCTAAATTGGTGTACAATGATAAAATGGTAGAAGAAAATTTTGAGATAAAGATGTGGATATGCGTCTCAGACAACTTTGTTATTCAAACATTAATTCGAGGTATTACTAATGCTGCAAATGGACCAAAATGTGAGGATGAAAGTTTGGATCTTATGGAAAGAAAGTTGCAAGATACTCTTAGAGGTAAAAAAATTTTATTGGTGTTGGACGATATCCGGGATTTAGAGTCGATTGGAGTAACAATTGAAAAATGGACTAATTTAAAAACTTTGTTAAATGTGGGAGCTAATGGAAGCAAAATCATTATAACAACACGAAATAAATCAGTTGCTCTACTTGTGAGTCCCTTATACATGCATTCTTTAGAAGGTCTTTCCCACAAAGATTGCATGTCCTTATTCATCCAAAGGGCATTTAAAAGAGGAGAGGAGCAACGCTATCAACATTTGATAGAAATCGGACAGGATATTGTGAAGAAGTGTGGAGGAGTTCCTTTAGCAAGTTACTACGTAGGGAGCATGCTCTATTTGAATAGAGAGCAACACCATTGGTTGCGTGTCGAGATGATGACATGTGGAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

409

Amino Acids

46.53

Weight (kDa)

8.24

Isoelectric Point (pI)

30.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 93 1.1e-18 Rx N-terminal domain
NB-ARC PF00931 177 - 356 2.5e-30 NB-ARC domain
AAA_22 PF13401 201 - 315 1.6e-06 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 953
AatII GACGTC 1 cut(s) 231
AccB1I GGYRCC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 276
AciI CCGC 1 cut(s) 259
AclWI GGATC 3 cut(s) 164, 443, 801
AcsI RAATTY 2 cut(s) 682, 837
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 2 cut(s) 306, 659
AfiI CCNNNNNNNGG 1 cut(s) 615
AflIII ACRYGT 1 cut(s) 1217
AgsI TTSAA 8 cut(s) 265, 349, 392, 513, 565, 731, 893, 1177
AhlI ACTAGT 2 cut(s) 133, 385
AluBI AGCT 6 cut(s) 47, 97, 106, 241, 443, 932
AluI AGCT 6 cut(s) 47, 97, 106, 241, 443, 932
Alw21I GWGCWC 3 cut(s) 99, 141, 474
Alw26I GTCTC 2 cut(s) 403, 715
Alw44I GTGCAC 1 cut(s) 470
AlwI GGATC 3 cut(s) 164, 443, 801
Ama87I CYCGRG 1 cut(s) 140
ApaLI GTGCAC 1 cut(s) 470
ApeKI GCWGC 2 cut(s) 15, 758
ApoI RAATTY 2 cut(s) 682, 837
AseI ATTAAT 1 cut(s) 737
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 1 cut(s) 767
AsuC2I CCSGG 1 cut(s) 863
AsuHPI GGTGA 1 cut(s) 91
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 1 cut(s) 767
BaeGI GKGCMC 2 cut(s) 88, 474
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 1 cut(s) 99
BarI GAAGNNNNNNTAC 2 cut(s) 31, 63
Bbv12I GWGCWC 3 cut(s) 99, 141, 474
BbvI GCAGC 2 cut(s) 27, 745
BccI CCATC 1 cut(s) 134
BcnI CCSGG 1 cut(s) 863
BcoDI GTCTC 2 cut(s) 403, 715
BcuI ACTAGT 2 cut(s) 133, 385
BfaI CTAG 3 cut(s) 44, 134, 386
BglII AGATCT 1 cut(s) 66
BisI GCNGC 2 cut(s) 16, 759
BlsI GCNGC 2 cut(s) 17, 760
Bme1390I CCNGG 1 cut(s) 863
Bme18I GGWCC 1 cut(s) 767
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 767
BmiI GGNNCC 1 cut(s) 85
BmrFI CCNGG 1 cut(s) 863
BmsI GCATC 2 cut(s) 136, 565
BmtI GCTAGC 1 cut(s) 47
BpmI CTGGAG 1 cut(s) 565
BpuEI CTTGAG 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 863
BsaAI YACGTR 1 cut(s) 1156
BsaBI GATNNNNATC 1 cut(s) 1100
BsaHI GRCGYC 1 cut(s) 228
Bsc4I CCNNNNNNNGG 1 cut(s) 615
Bse1I ACTGG 2 cut(s) 332, 582
Bse3DI GCAATG 1 cut(s) 18
Bse8I GATNNNNATC 1 cut(s) 1100
BseGI GGATG 4 cut(s) 151, 492, 787, 1047
BseJI GATNNNNATC 1 cut(s) 1100
BseLI CCNNNNNNNGG 1 cut(s) 615
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 1 cut(s) 726
BseNI ACTGG 2 cut(s) 332, 582
BseRI GAGGAG 4 cut(s) 602, 1083, 1088, 1146
BseSI GKGCMC 2 cut(s) 88, 474
BseXI GCAGC 2 cut(s) 27, 745
BshNI GGYRCC 1 cut(s) 83
BsiHKAI GWGCWC 3 cut(s) 99, 141, 474
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 1 cut(s) 862
BslFI GGGAC 1 cut(s) 975
BslI CCNNNNNNNGG 1 cut(s) 615
BsmAI GTCTC 2 cut(s) 403, 715
BsmBI CGTCTC 1 cut(s) 715
BsmFI GGGAC 1 cut(s) 975
BsmI GAATGC 1 cut(s) 1003
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 4 cut(s) 88, 99, 141, 474
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 5 cut(s) 66, 169, 291, 448, 793
BspACI CCGC 1 cut(s) 259
BspCNI CTCAG 1 cut(s) 725
BspHI TCATGA 1 cut(s) 214
BspLI GGNNCC 1 cut(s) 85
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 3 cut(s) 164, 443, 801
BspT107I GGYRCC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 18
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 2 cut(s) 332, 582
BssMI GATC 5 cut(s) 66, 169, 291, 448, 793
BssNI GRCGYC 1 cut(s) 228
Bst4CI ACNGT 2 cut(s) 284, 414
Bst6I CTCTTC 1 cut(s) 520
BstACI GRCGYC 1 cut(s) 228
BstAPI GCANNNNNTGC 1 cut(s) 83
BstAUI TGTACA 1 cut(s) 657
BstBAI YACGTR 1 cut(s) 1156
BstC8I GCNNGC 3 cut(s) 13, 45, 1166
BstDEI CTNAG 4 cut(s) 150, 435, 712, 824
BstF5I GGATG 4 cut(s) 151, 492, 787, 1047
BstHHI GCGC 1 cut(s) 460
BstKTI GATC 5 cut(s) 69, 172, 294, 451, 796
BstMAI GTCTC 2 cut(s) 403, 715
BstMBI GATC 5 cut(s) 66, 169, 291, 448, 793
BstMWI GCNNNNNNNGC 3 cut(s) 53, 83, 103
BstNSI RCATGY 5 cut(s) 56, 1003, 1039, 1168, 1221
BstSCI CCNGG 1 cut(s) 861
BstSLI GKGCMC 2 cut(s) 88, 474
BstSNI TACGTA 1 cut(s) 1156
BstV1I GCAGC 2 cut(s) 27, 745
BstX2I RGATCY 4 cut(s) 66, 169, 448, 793
BstYI RGATCY 4 cut(s) 66, 169, 448, 793
BtsCI GGATG 4 cut(s) 151, 492, 787, 1047
BtsIMutI CAGTG 2 cut(s) 570, 589
Cac8I GCNNGC 3 cut(s) 13, 45, 1166
CciI TCATGA 1 cut(s) 214
CfoI GCGC 1 cut(s) 460
Cfr13I GGNCC 1 cut(s) 767
CseI GACGC 1 cut(s) 697
Csp6I GTAC 2 cut(s) 305, 658
CspCI CAANNNNNGTGG 2 cut(s) 1013, 1048
CviAII CATG 7 cut(s) 53, 78, 215, 1000, 1036, 1165, 1218
CviJI RGCY 8 cut(s) 43, 47, 97, 106, 241, 443, 509, 932
CviKI_1 RGCY 8 cut(s) 43, 47, 97, 106, 241, 443, 509, 932
CviQI GTAC 2 cut(s) 305, 658
DdeI CTNAG 4 cut(s) 150, 435, 712, 824
DpnI GATC 5 cut(s) 68, 171, 293, 450, 795
DpnII GATC 5 cut(s) 66, 169, 291, 448, 793
DraI TTTAAA 2 cut(s) 909, 1063
Eam1104I CTCTTC 1 cut(s) 520
EarI CTCTTC 1 cut(s) 520
Ecl136II GAGCTC 1 cut(s) 97
Eco105I TACGTA 1 cut(s) 1156
Eco24I GRGCYC 1 cut(s) 99
Eco32I GATATC 1 cut(s) 859
Eco47I GGWCC 1 cut(s) 767
Eco53kI GAGCTC 1 cut(s) 97
Eco88I CYCGRG 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 97
EcoRV GATATC 1 cut(s) 859
EcoT22I ATGCAT 1 cut(s) 1005
EcoT38I GRGCYC 1 cut(s) 99
Esp3I CGTCTC 1 cut(s) 715
FaeI CATG 7 cut(s) 56, 81, 218, 1003, 1039, 1168, 1221
FalI AAGNNNNNCTT 4 cut(s) 545, 577, 807, 839
FaqI GGGAC 1 cut(s) 975
FatI CATG 7 cut(s) 52, 77, 214, 999, 1035, 1164, 1217
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 2 cut(s) 16, 759
FokI GGATG 4 cut(s) 158, 499, 794, 1034
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 2 cut(s) 16, 759
FspBI CTAG 3 cut(s) 44, 134, 386
GlaI GCGC 1 cut(s) 459
GluI GCNGC 2 cut(s) 16, 759
GsuI CTGGAG 1 cut(s) 565
HapII CCGG 1 cut(s) 862
HgaI GACGC 1 cut(s) 697
HhaI GCGC 1 cut(s) 460
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 7 cut(s) 56, 81, 218, 1003, 1039, 1168, 1221
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 3 cut(s) 328, 872, 988
HpaII CCGG 1 cut(s) 862
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 4 cut(s) 277, 307, 472, 658
Hpy188I TCNGA 2 cut(s) 715, 1106
Hpy188III TCNNGA 3 cut(s) 215, 562, 1207
Hpy8I GTNNAC 4 cut(s) 277, 307, 472, 658
HpyAV CCTTC 1 cut(s) 1007
HpyCH4III ACNGT 2 cut(s) 284, 414
HpyCH4IV ACGT 4 cut(s) 30, 219, 228, 1155
HpyCH4V TGCA 6 cut(s) 23, 472, 761, 814, 1003, 1035
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 83, 103
HpyF3I CTNAG 4 cut(s) 150, 435, 712, 824
HpySE526I ACGT 4 cut(s) 30, 219, 228, 1155
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 7 cut(s) 56, 81, 218, 1003, 1039, 1168, 1221
HspAI GCGC 1 cut(s) 458
Kzo9I GATC 5 cut(s) 66, 169, 291, 448, 793
LmnI GCTCC 3 cut(s) 929, 1075, 1161
LpnPI CCDG 7 cut(s) 25, 227, 345, 466, 595, 875, 1094
Lsp1109I GCAGC 2 cut(s) 27, 745
LweI GCATC 2 cut(s) 136, 565
MaeI CTAG 3 cut(s) 44, 134, 386
MaeII ACGT 4 cut(s) 30, 219, 228, 1155
MaeIII GTNAC 2 cut(s) 883, 1148
MalI GATC 5 cut(s) 68, 171, 293, 450, 795
MboI GATC 5 cut(s) 66, 169, 291, 448, 793
MboII GAAGA 5 cut(s) 166, 301, 537, 689, 1132
MfeI CAATTG 1 cut(s) 888
MflI RGATCY 4 cut(s) 66, 169, 448, 793
MhlI GDGCHC 4 cut(s) 88, 99, 141, 474
MluCI AATT 9 cut(s) 311, 393, 612, 650, 682, 738, 837, 888, 904
MlyI GAGTC 2 cut(s) 881, 997
MmeI TCCRAC 1 cut(s) 831
Mph1103I ATGCAT 1 cut(s) 1005
MseI TTAA 6 cut(s) 342, 366, 737, 908, 920, 1062
MspI CCGG 1 cut(s) 862
MspR9I CCNGG 1 cut(s) 863
MunI CAATTG 1 cut(s) 888
Mva1269I GAATGC 1 cut(s) 1003
MwoI GCNNNNNNNGC 3 cut(s) 53, 83, 103
NciI CCSGG 1 cut(s) 863
NdeII GATC 5 cut(s) 66, 169, 291, 448, 793
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 7 cut(s) 56, 81, 218, 1003, 1039, 1168, 1221
NlaIV GGNNCC 1 cut(s) 85
NsiI ATGCAT 1 cut(s) 1005
NspI RCATGY 5 cut(s) 56, 1003, 1039, 1168, 1221
PaeI GCATGC 1 cut(s) 1168
PaeR7I CTCGAG 1 cut(s) 140
PagI TCATGA 1 cut(s) 214
PciI ACATGT 1 cut(s) 1217
PcsI WCGNNNNNNNCGW 2 cut(s) 225, 234
PctI GAATGC 1 cut(s) 1003
PfeI GAWTC 1 cut(s) 328
PfoI TCCNGGA 1 cut(s) 861
PkrI GCNGC 2 cut(s) 17, 760
PleI GAGTC 2 cut(s) 880, 996
PpsI GAGTC 2 cut(s) 880, 996
Ppu21I YACGTR 1 cut(s) 1156
PscI ACATGT 1 cut(s) 1217
PshBI ATTAAT 1 cut(s) 737
PsiI TTATAA 1 cut(s) 953
Psp124BI GAGCTC 1 cut(s) 99
PspN4I GGNNCC 1 cut(s) 85
PspPI GGNCC 1 cut(s) 767
PspXI VCTCGAGB 1 cut(s) 140
PsuI RGATCY 4 cut(s) 66, 169, 448, 793
RsaI GTAC 2 cut(s) 306, 659
RsaNI GTAC 2 cut(s) 305, 658
SacI GAGCTC 1 cut(s) 99
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 6 cut(s) 342, 366, 737, 908, 920, 1062
SatI GCNGC 2 cut(s) 16, 759
Sau3AI GATC 5 cut(s) 66, 169, 291, 448, 793
Sau96I GGNCC 1 cut(s) 767
SchI GAGTC 2 cut(s) 881, 997
ScrFI CCNGG 1 cut(s) 863
SduI GDGCHC 4 cut(s) 88, 99, 141, 474
SfaNI GCATC 2 cut(s) 136, 565
Sfr274I CTCGAG 1 cut(s) 140
SinI GGWCC 1 cut(s) 767
SlaI CTCGAG 1 cut(s) 140
SmlI CTYRAG 2 cut(s) 92, 140
SmoI CTYRAG 2 cut(s) 92, 140
SnaBI TACGTA 1 cut(s) 1156
SpeI ACTAGT 2 cut(s) 133, 385
SphI GCATGC 1 cut(s) 1168
Sse9I AATT 9 cut(s) 311, 393, 612, 650, 682, 738, 837, 888, 904
SsiI CCGC 1 cut(s) 259
SspMI CTAG 3 cut(s) 44, 134, 386
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 1 cut(s) 861
TaaI ACNGT 2 cut(s) 284, 414
TaiI ACGT 4 cut(s) 33, 222, 231, 1158
TaqI TCGA 5 cut(s) 141, 276, 742, 875, 1206
TasI AATT 9 cut(s) 311, 393, 612, 650, 682, 738, 837, 888, 904
TatI WGTACW 1 cut(s) 657
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 6 cut(s) 342, 366, 737, 908, 920, 1062
Tru9I TTAA 6 cut(s) 342, 366, 737, 908, 920, 1062
TscAI CASTG 2 cut(s) 577, 589
TseI GCWGC 2 cut(s) 15, 758
TspDTI ATGAA 3 cut(s) 417, 798, 1036
TspRI CASTG 2 cut(s) 577, 589
VneI GTGCAC 1 cut(s) 470
VpaK11BI GGWCC 1 cut(s) 767
VspI ATTAAT 1 cut(s) 737
XapI RAATTY 2 cut(s) 682, 837
XceI RCATGY 5 cut(s) 56, 1003, 1039, 1168, 1221
XhoI CTCGAG 1 cut(s) 140
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 3 cut(s) 44, 134, 386
ZraI GACGTC 1 cut(s) 229
Zsp2I ATGCAT 1 cut(s) 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.