Rorug06G0421600

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000159
Physical Location & Seq
Reverse (-)
56001298 .. 56004175
2878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0421600.1

Sequence Viewer

Length: 1335 bp
ATGAACCCTTTGTTGCTCTGTCTTCTAACTCTTCTTCTTGTCTCCCATCCTTGCCTTTCTTCTTCTTCTTCTTCTTCTTCTTATTCTTCTTCTTCTTCATCTGAACTGTTTGAAGCTTGGTGCAAACAGTATGGCAAGTCATACTCTTCAGAACAAGAAAAGCTCTATAGGCTTAGTGTGTTTGAGCAAAATCTTGCCTTCATTACTCAACACAATGATTTGGGCAATTCTTCTTACACCCTTTCTCTCAATTCATTTTCTGATCTTGCCCACCACGAGTTTAAGGCCTCTCGGTTAGGATTTTCGCCTACGTTTCTCAGACTTAACCGGAAATCAGACCCAAAACCCAGTGCGGTTCGTGATGTTTCTTCTTCGATTGATTGGAGGAAGAATGGAGCTGTGACCAATGTTAAAGATCAAGGCAGTTGTGGTGCTTGTTGGTCCTTTTCAGCAACTGGTGCCATTGAAGGTATCAACAAGATTGTCACTGGATCACTTGTCAGCCTCTCCGAACAAGAGTTGATTGATTGTGACAGAGTTTATCCAAATAGCGGCTGTGATGGTGGACTGATGGACGATGCATTTCAGTTTATCATAGATAACAATGGGATTGACACTGAGGAGGATTACCCGTATCGAGGTGTGGATGGCACTTGCAATAAGCAAAGGTTGAAGCGGCATGTTGTGACAATTGATGGCTATACTGATGTGCCCGCAAATGATGAGGAACAACTCCTACAGGCTGTGGCAAGGCAACCTGTGAGTGTAGGTATAGCTGGCAGTGGCAGGGAATTTCAGTTCTACTCAAAGGGGATTTTTGCTGGCCCTTGTTCAACTTCTTTGGATCATGCTGTATTAATTGTAGGTTATGGTTCAGAAAATGGGGTGAACTATTGGATTGTAAAGAATTCATGGGGTAAGAACTGGGGAATGAATGGCTATATTCACATGCTGCGTGATCATAGCAATTCCAAAGGGGTCTGCGGAATCAACATGTTGGCTTCATATCCAACAAAGACTGGGGAAAACCCTCCTTTTCCCCCGCCTTCCCCTCCTCCAGGTCCAACTAAATGTGATATCTTTTCCAAGTGTGGAGTTGGGGAAACCTGCTGCTGTGCAAAGAAGTTTTTTGGAATATGCCTTTCTTGGAGATGCTGCGAGCTAGCCTCTGCTGTTTGTTGTAAGGATCAACTTCACTGTTGCCCCCATGATTATCCTATTTGTGATACAGAGAGGAACTTCTGCCTCCAGGCTAACGGGAATTTAACAATGAAAGCAAATGAGAGTAGAGGCAGTTTGGGAAAGTCAAGCCGATCGAAAGCAAAGTTGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

444

Amino Acids

48.56

Weight (kDa)

6.55

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 37 - 94 1.1e-20 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 123 - 338 5.9e-83 Papain family cysteine protease
Granulin PF00396 369 - 416 1.7e-09 Granulin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1115
AccB1I GGYRCC 1 cut(s) 458
AciI CCGC 6 cut(s) 353, 552, 676, 714, 984, 1043
AclWI GGATC 3 cut(s) 499, 852, 1194
AcsI RAATTY 3 cut(s) 791, 907, 1261
AcuI CTGAAG 1 cut(s) 132
AfiI CCNNNNNNNGG 3 cut(s) 551, 638, 1058
AflIII ACRYGT 1 cut(s) 993
AgsI TTSAA 4 cut(s) 113, 467, 673, 834
AjnI CCWGG 2 cut(s) 1057, 1248
AloI GAACNNNNNNTCC 2 cut(s) 782, 814
AluBI AGCT 5 cut(s) 116, 163, 398, 776, 1162
AluI AGCT 5 cut(s) 116, 163, 398, 776, 1162
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 3 cut(s) 499, 852, 1194
AlwNI CAGNNNCTG 1 cut(s) 455
AoxI GGCC 2 cut(s) 285, 823
ApeKI GCWGC 3 cut(s) 952, 1110, 1155
ApoI RAATTY 3 cut(s) 791, 907, 1261
AseI ATTAAT 1 cut(s) 857
AspS9I GGNCC 3 cut(s) 441, 824, 1061
AsuHPI GGTGA 1 cut(s) 898
AsuNHI GCTAGC 1 cut(s) 1162
AvaII GGWCC 2 cut(s) 441, 1061
BaeGI GKGCMC 1 cut(s) 714
BanI GGYRCC 1 cut(s) 458
BauI CACGAG 1 cut(s) 275
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 3 cut(s) 939, 1097, 1142
BccI CCATC 5 cut(s) 54, 554, 565, 641, 689
BciT130I CCWGG 2 cut(s) 1059, 1250
BclI TGATCA 1 cut(s) 958
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 2 cut(s) 1163, 1333
BfmI CTRYAG 2 cut(s) 166, 737
BfuAI ACCTGC 1 cut(s) 1115
BisI GCNGC 5 cut(s) 553, 677, 953, 1111, 1156
BlsI GCNGC 5 cut(s) 554, 678, 954, 1112, 1157
Bme1390I CCNGG 2 cut(s) 1059, 1250
Bme18I GGWCC 2 cut(s) 441, 1061
BmgT120I GGNCC 3 cut(s) 441, 824, 1061
BmiI GGNNCC 1 cut(s) 460
BmrFI CCNGG 2 cut(s) 1059, 1250
BmrI ACTGGG 3 cut(s) 342, 934, 1029
BmsI GCATC 2 cut(s) 568, 1142
BmtI GCTAGC 1 cut(s) 1166
BmuI ACTGGG 3 cut(s) 342, 934, 1029
BpiI GAAGAC 1 cut(s) 14
BplI GAGNNNNNCTC 2 cut(s) 1151, 1183
BpmI CTGGAG 2 cut(s) 1041, 1232
BsaWI WCCGGW 1 cut(s) 327
Bsc4I CCNNNNNNNGG 3 cut(s) 551, 638, 1058
Bse1I ACTGG 5 cut(s) 348, 460, 493, 929, 1024
BseBI CCWGG 2 cut(s) 1059, 1250
BseGI GGATG 2 cut(s) 46, 652
BseLI CCNNNNNNNGG 3 cut(s) 551, 638, 1058
BseMII CTCAG 2 cut(s) 331, 609
BseNI ACTGG 5 cut(s) 348, 460, 493, 929, 1024
BseRI GAGGAG 2 cut(s) 635, 1044
BseSI GKGCMC 1 cut(s) 714
BseXI GCAGC 3 cut(s) 939, 1097, 1142
Bsh1285I CGRYCG 1 cut(s) 1316
BshFI GGCC 2 cut(s) 287, 825
BshNI GGYRCC 1 cut(s) 458
BsiEI CGRYCG 1 cut(s) 1316
BsiSI CCGG 1 cut(s) 328
BslI CCNNNNNNNGG 3 cut(s) 551, 638, 1058
BsmAI GTCTC 1 cut(s) 46
BsnI GGCC 2 cut(s) 287, 825
Bsp1286I GDGCHC 1 cut(s) 714
Bsp143I GATC 7 cut(s) 262, 415, 491, 844, 958, 1186, 1313
BspACI CCGC 6 cut(s) 353, 552, 676, 714, 984, 1043
BspANI GGCC 2 cut(s) 287, 825
BspCNI CTCAG 2 cut(s) 330, 610
BspLI GGNNCC 1 cut(s) 460
BspMI ACCTGC 1 cut(s) 1115
BspOI GCTAGC 1 cut(s) 1166
BspPI GGATC 3 cut(s) 499, 852, 1194
BspT107I GGYRCC 1 cut(s) 458
BsrI ACTGG 5 cut(s) 348, 460, 493, 929, 1024
BssMI GATC 7 cut(s) 262, 415, 491, 844, 958, 1186, 1313
BssSI CACGAG 1 cut(s) 275
Bst2BI CACGAG 1 cut(s) 275
Bst2UI CCWGG 2 cut(s) 1059, 1250
Bst4CI ACNGT 3 cut(s) 108, 129, 1199
Bst6I CTCTTC 2 cut(s) 36, 151
BstAPI GCANNNNNTGC 1 cut(s) 458
BstC8I GCNNGC 5 cut(s) 714, 778, 823, 1160, 1164
BstDEI CTNAG 3 cut(s) 173, 317, 618
BstENI CCTNNNNNAGG 1 cut(s) 1056
BstF5I GGATG 2 cut(s) 46, 652
BstKTI GATC 7 cut(s) 265, 418, 494, 847, 961, 1189, 1316
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 7 cut(s) 262, 415, 491, 844, 958, 1186, 1313
BstMCI CGRYCG 1 cut(s) 1316
BstMWI GCNNNNNNNGC 2 cut(s) 169, 458
BstNI CCWGG 2 cut(s) 1059, 1250
BstNSI RCATGY 3 cut(s) 683, 952, 997
BstSCI CCNGG 2 cut(s) 1057, 1248
BstSFI CTRYAG 2 cut(s) 166, 737
BstSLI GKGCMC 1 cut(s) 714
BstV1I GCAGC 3 cut(s) 939, 1097, 1142
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 2 cut(s) 287, 825
BtsCI GGATG 2 cut(s) 46, 652
BtsI GCAGTG 1 cut(s) 787
BtsIMutI CAGTG 5 cut(s) 355, 486, 615, 787, 1195
BveI ACCTGC 1 cut(s) 1115
Cac8I GCNNGC 5 cut(s) 714, 778, 823, 1160, 1164
CaiI CAGNNNCTG 1 cut(s) 455
Cfr13I GGNCC 3 cut(s) 441, 824, 1061
CviAII CATG 6 cut(s) 680, 848, 912, 949, 994, 1208
DdeI CTNAG 3 cut(s) 173, 317, 618
DpnI GATC 7 cut(s) 264, 417, 493, 846, 960, 1188, 1315
DpnII GATC 7 cut(s) 262, 415, 491, 844, 958, 1186, 1313
Eam1104I CTCTTC 2 cut(s) 36, 151
EarI CTCTTC 2 cut(s) 36, 151
Eco147I AGGCCT 1 cut(s) 287
Eco32I GATATC 1 cut(s) 1078
Eco47I GGWCC 2 cut(s) 441, 1061
Eco57I CTGAAG 1 cut(s) 132
EcoNI CCTNNNNNAGG 1 cut(s) 1056
EcoRI GAATTC 1 cut(s) 907
EcoRII CCWGG 2 cut(s) 1057, 1248
EcoRV GATATC 1 cut(s) 1078
EcoT22I ATGCAT 1 cut(s) 583
FaeI CATG 6 cut(s) 683, 851, 915, 952, 997, 1211
FatI CATG 6 cut(s) 679, 847, 911, 948, 993, 1207
FauI CCCGC 2 cut(s) 721, 1050
FbaI TGATCA 1 cut(s) 958
Fnu4HI GCNGC 5 cut(s) 553, 677, 953, 1111, 1156
FokI GGATG 2 cut(s) 33, 659
Fsp4HI GCNGC 5 cut(s) 553, 677, 953, 1111, 1156
FspBI CTAG 2 cut(s) 1163, 1333
GluI GCNGC 5 cut(s) 553, 677, 953, 1111, 1156
GsuI CTGGAG 2 cut(s) 1041, 1232
HaeIII GGCC 2 cut(s) 287, 825
HapII CCGG 1 cut(s) 328
Hin1II CATG 6 cut(s) 683, 851, 915, 952, 997, 1211
HindIII AAGCTT 1 cut(s) 114
HinfI GANTC 1 cut(s) 987
HpaII CCGG 1 cut(s) 328
HphI GGTGA 1 cut(s) 898
Hpy166II GTNNAC 2 cut(s) 566, 889
Hpy188I TCNGA 7 cut(s) 103, 151, 262, 320, 337, 511, 877
Hpy188III TCNNGA 1 cut(s) 359
Hpy8I GTNNAC 2 cut(s) 566, 889
HpyAV CCTTC 3 cut(s) 208, 461, 1056
HpyCH4III ACNGT 3 cut(s) 108, 129, 1199
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 4 cut(s) 123, 581, 657, 1118
HpyF10VI GCNNNNNNNGC 2 cut(s) 169, 458
HpyF3I CTNAG 3 cut(s) 173, 317, 618
HpySE526I ACGT 1 cut(s) 311
Hsp92II CATG 6 cut(s) 683, 851, 915, 952, 997, 1211
Ksp22I TGATCA 1 cut(s) 958
Kzo9I GATC 7 cut(s) 262, 415, 491, 844, 958, 1186, 1313
LmnI GCTCC 1 cut(s) 395
Lsp1109I GCAGC 3 cut(s) 939, 1097, 1142
LweI GCATC 2 cut(s) 568, 1142
MaeI CTAG 2 cut(s) 1163, 1333
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 4 cut(s) 400, 484, 530, 685
MalI GATC 7 cut(s) 264, 417, 493, 846, 960, 1188, 1315
MboI GATC 7 cut(s) 262, 415, 491, 844, 958, 1186, 1313
MfeI CAATTG 1 cut(s) 690
MhlI GDGCHC 1 cut(s) 714
MluCI AATT 8 cut(s) 226, 250, 690, 791, 858, 907, 967, 1261
MmeI TCCRAC 2 cut(s) 1034, 1088
Mph1103I ATGCAT 1 cut(s) 583
MseI TTAA 5 cut(s) 282, 324, 411, 857, 1265
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 2 cut(s) 1059, 1250
MunI CAATTG 1 cut(s) 690
MvaI CCWGG 2 cut(s) 1059, 1250
MwoI GCNNNNNNNGC 2 cut(s) 169, 458
NdeII GATC 7 cut(s) 262, 415, 491, 844, 958, 1186, 1313
NheI GCTAGC 1 cut(s) 1162
NlaIII CATG 6 cut(s) 683, 851, 915, 952, 997, 1211
NlaIV GGNNCC 1 cut(s) 460
NmuCI GTSAC 4 cut(s) 400, 484, 530, 685
NsiI ATGCAT 1 cut(s) 583
NspI RCATGY 3 cut(s) 683, 952, 997
PceI AGGCCT 1 cut(s) 287
PciI ACATGT 1 cut(s) 993
PfeI GAWTC 1 cut(s) 987
PkrI GCNGC 5 cut(s) 554, 678, 954, 1112, 1157
Ple19I CGATCG 1 cut(s) 1316
PscI ACATGT 1 cut(s) 993
PshBI ATTAAT 1 cut(s) 857
Psp6I CCWGG 2 cut(s) 1057, 1248
PspGI CCWGG 2 cut(s) 1057, 1248
PspN4I GGNNCC 1 cut(s) 460
PspPI GGNCC 3 cut(s) 441, 824, 1061
PsrI GAACNNNNNNTAC 2 cut(s) 720, 752
PstNI CAGNNNCTG 1 cut(s) 455
PvuI CGATCG 1 cut(s) 1316
SaqAI TTAA 5 cut(s) 282, 324, 411, 857, 1265
SatI GCNGC 5 cut(s) 553, 677, 953, 1111, 1156
Sau3AI GATC 7 cut(s) 262, 415, 491, 844, 958, 1186, 1313
Sau96I GGNCC 3 cut(s) 441, 824, 1061
ScrFI CCNGG 2 cut(s) 1059, 1250
SduI GDGCHC 1 cut(s) 714
SfaNI GCATC 2 cut(s) 568, 1142
SfcI CTRYAG 2 cut(s) 166, 737
SinI GGWCC 2 cut(s) 441, 1061
Sse9I AATT 8 cut(s) 226, 250, 690, 791, 858, 907, 967, 1261
SseBI AGGCCT 1 cut(s) 287
SsiI CCGC 6 cut(s) 353, 552, 676, 714, 984, 1043
SspMI CTAG 2 cut(s) 1163, 1333
StuI AGGCCT 1 cut(s) 287
StyD4I CCNGG 2 cut(s) 1057, 1248
TaaI ACNGT 3 cut(s) 108, 129, 1199
TaiI ACGT 1 cut(s) 314
TaqI TCGA 3 cut(s) 374, 637, 1316
TasI AATT 8 cut(s) 226, 250, 690, 791, 858, 907, 967, 1261
TauI GCSGC 2 cut(s) 555, 679
TfiI GAWTC 1 cut(s) 987
Tru1I TTAA 5 cut(s) 282, 324, 411, 857, 1265
Tru9I TTAA 5 cut(s) 282, 324, 411, 857, 1265
TscAI CASTG 5 cut(s) 355, 493, 622, 787, 1202
TseFI GTSAC 4 cut(s) 400, 484, 530, 685
TseI GCWGC 3 cut(s) 952, 1110, 1155
Tsp45I GTSAC 4 cut(s) 400, 484, 530, 685
TspDTI ATGAA 8 cut(s) 17, 87, 190, 243, 900, 947, 993, 1286
TspRI CASTG 5 cut(s) 355, 493, 622, 787, 1202
VpaK11BI GGWCC 2 cut(s) 441, 1061
VspI ATTAAT 1 cut(s) 857
XagI CCTNNNNNAGG 1 cut(s) 1056
XapI RAATTY 3 cut(s) 791, 907, 1261
XceI RCATGY 3 cut(s) 683, 952, 997
XspI CTAG 2 cut(s) 1163, 1333
Zsp2I ATGCAT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.