Rh7BG020200

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000159
Physical Location & Seq
Reverse (-)
1343188 .. 1346748
3561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG020200.1

Sequence Viewer

Length: 2550 bp
ATGGAGTTTGCCTCTGGCATTGCTGAGACCGTCTCGGTTAGGCTAGCCTCCTATGCTGCCCACGAAGTTAGCTTGGCTTGGGGTGCCCAACATGAGCTGAAGAAGCTCAAAAAGACCTTGTCAGCCATCAAACTTGTGCTTCAGGATGCAGAGAAGAAGCAATTGCAGAATCCCCTCATCACTCATTGGTTAGGAAACCTCAAAGATGTTTGCAATGATGCCGAGGATGTACTGGACGAATTTGAGTTCCGAAAGCTCCGACTCAAAGTGCAACGGAGCGTCAAAGGAAAGGTACACCAATTCTTCTCCTGTTGGAATCCAGTCGTGTTTAATTTGAAAATGGGTCATAAAATTAAAGAGATTAGAGAAAGACTAGTTGAGATTGATGATGAAAAGACACAATTTTCTCTCGATAAAGTTGCTGAAGTACCCAACGATATTAGAAGGGAGACAGACTCTTTACCAGAGGCGTATATTGTTGGAAGAGATGAGGACAGAAATCAGATTATTAACCATCTCTTGAATGGCACCCGTTCTTTTGGTAATGAGAATGTTTCAGTTATCTCCATCATCGGGTTAGGTGGGTTGGGAAAAACTACACTTGCTAAAATGGTGTACAATGATAATGTGGTAGAAGAAAAATTTGAGATGAGGTTGTGGATATGTGTCTCTGATAAATTTGATATCCATACATTAGTTAAAGGCATTATTACTGAAACTAAAAAGGAATGTGATTGTGGAAGTTTGAACCTTATGAAAAAGGGGTTGCAAGATATCTTGAGAGGTACAAAGTTTTTGTTAGTCTTGGATGATGTTTGGGACACAGAGTCAACAGGATTTACAGTTCAAAAATGGGATGAGTTAATAACTTTGTTAGATGTTGGAGCCAAAGGGAGTAAAATCATCGTGACAACACGCAGTGAATTAGTCGGTTCACTTGTACACCCAATACAAATGCATTCGTTAGAAGGTCTTTCTCATGAGGATTCCATGATCTTGTTTAAAAAAAATGCATTTTACGTGGGAGAGGAGCAACTATATCAACACTTGATAGAAATTGGGGAGGACATTGTGAAGAAGTGTGGAGGTGTTCCCTTAGCACTAGTTACTCTAGGGAGCTTGCTCCGCTCGAAAAGGGTGCTACGCGACTGGGTACGTGTGAAAGATGATGAAATATGGAGTATGTTAGGAAACGACACCATTATGGCTGCACTAAAATTGAGTTACAATGCATTGCCGCCTCACTTGAAATCTTGTTTTGCCTTTTGTTCACTTTACCCCAAGGATTATGTATTTATTAGTGAAGACATAGTCCCATTATGGATGGCACAAGGCTATATCAAGTCCTTAGAAGAAAATGAAGATTTTGAAGAGACGGGTCTAGACTATATAAGGCTGTTTTGTTCCAAGTCCTTGTTTCAACTTGAGTTAGATTTGAAGACTAACATAGTGTTCAAAATTCATGATCTATTGCACGATTTAGCAATATCAGTGGCAGGGGTAGAGTATTCCACGGTTAATTGTCGTCCCTCAAGTGCTTTTGAAAAAGTCCGGCACATATCAATAACGGCAAAAGACTTGCCTGGAGATGAGGCACGAGTCCCGAAATTCATTCTCCAGTTGGAAAAACTACGAACCATTTTAAATGGAGATATGGATGCAGGAATCTCCAATCAATATTTTCTAAGGACATGCATCTCGAGATTCAAGTATATGCGTGCACTAGATATACGTGGTTCAACTCTTGAGGAGCTACCAAGTTCCATTGGTAACTTGAGTCATTTGAGATATCTCAACCTGGATGATAATACAAAGTTAAGAAAGCTTCCCGCTTCCATTGGCAAGCTCTTGAATTTGCAGTTCTTAAGCCTTGAAGATTGTGAGGCACTTGAGGAGTTACCCAAAGGCATAGGGAACCTGATCAGTCTCAGATTTTTGGATATAACTACGCAGCAGATGTATTTGCCGAAAGGATTATTCAGACGCCTCACCTTGCTTCTAACATTATACGTCTCGAGCTGTGAAAATCTAAAATCTCTGGGTGAAGAGATGGAATACCTCACTAACCTTCGTCAGCTGGGGATTAGAAACTGTGGAAATCTGGAGTCCTTGCCACCGAACACAAAGAACTTGACTGCATTACATACTTTGGTGATCGGTGATTGTAAGAAGCTTGAGTTAATGAGGTCGGGAGAAGGCATCAATGGTCTCAGATCATTCGCTGTCAACGGATCAGATTTGGAGGCTTTGCCGAAGTGGCTTCAAGAGTCTGCACACACTTTACAGAGCATACTAATCAGCGAGTGTGATAATCTCACGGCACTTCCAGAGTGGTTACAAAATCTCACCTTGCTGGAGCAACTGGTGATTGTAGACTGCCCCAAATTGCCTGCTTTGCCGCAAGGTATGCATTGCCTCACTGTGTTGAGAGAATTGCGGATTCTTAATTGTACTAAATTGAGAAAAAGATGCGAAAGGGAAACGGGAGAGGATTGGTCCAAGATCAAACATGTCCCAAAGATTGTACTCGACGACTCTGACGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

849

Amino Acids

96.27

Weight (kDa)

5.89

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 13 - 93 9.2e-22 Rx N-terminal domain
NB-ARC PF00931 166 - 339 7.5e-31 NB-ARC domain
WHD_DRP PF23559 425 - 495 2.8e-17 Disease resistance protein Winged helix domain
LRR_14 PF23598 566 - 763 3.3e-25 Leucine-rich repeat region
LRR_13 PF23286 580 - 727 2.2e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 83, 527
AccBSI CCGCTC 1 cut(s) 1128
AccI GTMKAC 1 cut(s) 2373
AccII CGCG 1 cut(s) 1146
AciI CCGC 5 cut(s) 1126, 1238, 1830, 2399, 2437
AclWI GGATC 1 cut(s) 2239
AcsI RAATTY 6 cut(s) 239, 641, 677, 1458, 1607, 1852
AcuI CTGAAG 3 cut(s) 119, 125, 444
AcyI GRCGYC 1 cut(s) 1984
AdeI CACNNNGTG 1 cut(s) 920
AfaI GTAC 9 cut(s) 231, 294, 429, 617, 787, 942, 1155, 2452, 2526
AfiI CCNNNNNNNGG 1 cut(s) 573
AflII CTTAAG 1 cut(s) 1864
AflIII ACRYGT 2 cut(s) 1156, 2509
AhdI GACNNNNNGTC 1 cut(s) 826
AhlI ACTAGT 2 cut(s) 373, 1102
AjnI CCWGG 2 cut(s) 1582, 1797
AloI GAACNNNNNNTCC 2 cut(s) 828, 860
Alw21I GWGCWC 1 cut(s) 1723
Alw26I GTCTC 8 cut(s) 20, 37, 443, 673, 1367, 1931, 2017, 2213
Alw44I GTGCAC 1 cut(s) 1719
AlwI GGATC 1 cut(s) 2239
Ama87I CYCGRG 2 cut(s) 1699, 2014
ApaLI GTGCAC 1 cut(s) 1719
ApeKI GCWGC 3 cut(s) 56, 1208, 1951
ApoI RAATTY 6 cut(s) 239, 641, 677, 1458, 1607, 1852
AspS9I GGNCC 1 cut(s) 2496
AsuHPI GGTGA 6 cut(s) 1981, 2054, 2164, 2171, 2338, 2377
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 2 cut(s) 1699, 2014
AvaII GGWCC 1 cut(s) 2496
BaeGI GKGCMC 2 cut(s) 88, 1723
BanI GGYRCC 2 cut(s) 83, 527
BauI CACGAG 1 cut(s) 1596
BbsI GAAGAC 2 cut(s) 1311, 1445
Bbv12I GWGCWC 1 cut(s) 1723
BbvI GCAGC 3 cut(s) 43, 1195, 1963
BccI CCATC 5 cut(s) 134, 522, 575, 1318, 2044
BceAI ACGGC 2 cut(s) 1584, 2334
BcgI CGANNNNNNTGC 4 cut(s) 401, 435, 1120, 1154
BciT130I CCWGG 2 cut(s) 1584, 1799
BclI TGATCA 1 cut(s) 1920
BcoDI GTCTC 8 cut(s) 20, 37, 443, 673, 1367, 1931, 2017, 2213
BcuI ACTAGT 2 cut(s) 373, 1102
BfaI CTAG 6 cut(s) 44, 374, 1103, 1112, 1382, 1724
BfrI CTTAAG 1 cut(s) 1864
BglI GCCNNNNNGGC 1 cut(s) 2257
BisI GCNGC 5 cut(s) 57, 1209, 1238, 1952, 2399
BlsI GCNGC 5 cut(s) 58, 1210, 1239, 1953, 2400
Bme1390I CCNGG 2 cut(s) 1584, 1799
Bme18I GGWCC 1 cut(s) 2496
BmeRI GACNNNNNGTC 1 cut(s) 826
BmeT110I CYCGRG 2 cut(s) 1699, 2014
BmgT120I GGNCC 1 cut(s) 2496
BmiI GGNNCC 4 cut(s) 85, 529, 886, 1916
BmrFI CCNGG 2 cut(s) 1584, 1799
BmrI ACTGGG 1 cut(s) 1159
BmsI GCATC 6 cut(s) 136, 208, 1648, 1704, 2208, 2459
BmtI GCTAGC 1 cut(s) 47
BmuI ACTGGG 1 cut(s) 1159
BpiI GAAGAC 2 cut(s) 1311, 1445
BplI GAGNNNNNCTC 5 cut(s) 28, 17, 49, 440, 472
BpmI CTGGAG 4 cut(s) 1601, 1605, 2123, 2375
Bpu10I CCTNAGC 1 cut(s) 1096
BpuEI CTTGAG 7 cut(s) 799, 1445, 1516, 1766, 1795, 1910, 2195
BsaAI YACGTR 3 cut(s) 1021, 1157, 1733
BsaHI GRCGYC 1 cut(s) 1984
BsaI GGTCTC 2 cut(s) 20, 2213
BsaJI CCNNGG 3 cut(s) 222, 1281, 1512
Bsc4I CCNNNNNNNGG 1 cut(s) 573
Bse1I ACTGG 5 cut(s) 237, 320, 1154, 1618, 2367
Bse3DI GCAATG 4 cut(s) 18, 220, 1232, 2410
BseBI CCWGG 2 cut(s) 1584, 1799
BseDI CCNNGG 3 cut(s) 222, 1281, 1512
BseGI GGATG 7 cut(s) 151, 232, 814, 862, 1329, 1663, 1807
BseLI CCNNNNNNNGG 1 cut(s) 573
BseMI GCAATG 4 cut(s) 18, 220, 1232, 2410
BseMII CTCAG 3 cut(s) 15, 1942, 2224
BseNI ACTGG 5 cut(s) 237, 320, 1154, 1618, 2367
BseRI GAGGAG 3 cut(s) 1043, 1763, 1907
BseSI GKGCMC 2 cut(s) 88, 1723
BseXI GCAGC 3 cut(s) 43, 1195, 1963
BseYI CCCAGC 1 cut(s) 2077
BsgI GTGCAG 2 cut(s) 1194, 2256
Bsh1236I CGCG 1 cut(s) 1146
BshNI GGYRCC 2 cut(s) 83, 527
BsiHKAI GWGCWC 1 cut(s) 1723
BsiHKCI CYCGRG 2 cut(s) 1699, 2014
BsiSI CCGG 1 cut(s) 1552
BslFI GGGAC 5 cut(s) 833, 1298, 1512, 1586, 2498
BslI CCNNNNNNNGG 1 cut(s) 573
BsmAI GTCTC 8 cut(s) 20, 37, 443, 673, 1367, 1931, 2017, 2213
BsmBI CGTCTC 3 cut(s) 37, 1367, 2017
BsmFI GGGAC 5 cut(s) 833, 1298, 1512, 1586, 2498
BsmI GAATGC 1 cut(s) 958
Bso31I GGTCTC 2 cut(s) 20, 2213
BsoBI CYCGRG 2 cut(s) 1699, 2014
Bsp1286I GDGCHC 2 cut(s) 88, 1723
Bsp1407I TGTACA 2 cut(s) 615, 940
Bsp143I GATC 7 cut(s) 993, 1465, 1920, 2154, 2213, 2231, 2502
BspACI CCGC 5 cut(s) 1126, 1238, 1830, 2399, 2437
BspCNI CTCAG 3 cut(s) 16, 1941, 2223
BspFNI CGCG 1 cut(s) 1146
BspHI TCATGA 2 cut(s) 979, 1462
BspLI GGNNCC 4 cut(s) 85, 529, 886, 1916
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 1 cut(s) 2239
BspT107I GGYRCC 2 cut(s) 83, 527
BspTI CTTAAG 1 cut(s) 1864
BspTNI GGTCTC 2 cut(s) 20, 2213
BsrBI CCGCTC 1 cut(s) 1128
BsrDI GCAATG 4 cut(s) 18, 220, 1232, 2410
BsrGI TGTACA 2 cut(s) 615, 940
BsrI ACTGG 5 cut(s) 237, 320, 1154, 1618, 2367
BssECI CCNNGG 3 cut(s) 222, 1281, 1512
BssMI GATC 7 cut(s) 993, 1465, 1920, 2154, 2213, 2231, 2502
BssNI GRCGYC 1 cut(s) 1984
BssSI CACGAG 1 cut(s) 1596
BssT1I CCWWGG 1 cut(s) 1281
Bst2BI CACGAG 1 cut(s) 1596
Bst2UI CCWGG 2 cut(s) 1584, 1799
Bst4CI ACNGT 5 cut(s) 31, 844, 1516, 2093, 2422
Bst6I CTCTTC 3 cut(s) 478, 1365, 2040
BstACI GRCGYC 1 cut(s) 1984
BstAFI CTTAAG 1 cut(s) 1864
BstAPI GCANNNNNTGC 1 cut(s) 2407
BstAUI TGTACA 2 cut(s) 615, 940
BstBAI YACGTR 3 cut(s) 1021, 1157, 1733
BstC8I GCNNGC 5 cut(s) 45, 1121, 1719, 1844, 2391
BstDEI CTNAG 6 cut(s) 24, 1096, 1348, 1685, 1928, 2210
BstDSI CCRYGG 1 cut(s) 1512
BstF5I GGATG 7 cut(s) 151, 232, 814, 862, 1329, 1663, 1807
BstFNI CGCG 1 cut(s) 1146
BstKTI GATC 7 cut(s) 996, 1468, 1923, 2157, 2216, 2234, 2505
BstMAI GTCTC 8 cut(s) 20, 37, 443, 673, 1367, 1931, 2017, 2213
BstMBI GATC 7 cut(s) 993, 1465, 1920, 2154, 2213, 2231, 2502
BstMWI GCNNNNNNNGC 7 cut(s) 53, 83, 103, 1125, 2257, 2395, 2407
BstNI CCWGG 2 cut(s) 1584, 1799
BstNSI RCATGY 2 cut(s) 1695, 2513
BstSCI CCNGG 2 cut(s) 1582, 1797
BstSLI GKGCMC 2 cut(s) 88, 1723
BstUI CGCG 1 cut(s) 1146
BstV1I GCAGC 3 cut(s) 43, 1195, 1963
BstV2I GAAGAC 2 cut(s) 1311, 1445
BtgI CCRYGG 1 cut(s) 1512
BtsCI GGATG 7 cut(s) 151, 232, 814, 862, 1329, 1663, 1807
BtsI GCAGTG 1 cut(s) 925
BtsIMutI CAGTG 3 cut(s) 925, 1497, 2418
Cac8I GCNNGC 5 cut(s) 45, 1121, 1719, 1844, 2391
CciI TCATGA 2 cut(s) 979, 1462
Cfr13I GGNCC 1 cut(s) 2496
CseI GACGC 2 cut(s) 268, 1992
Csp6I GTAC 9 cut(s) 230, 293, 428, 616, 786, 941, 1154, 2451, 2525
CspCI CAANNNNNGTGG 2 cut(s) 1473, 1508
CviAII CATG 6 cut(s) 92, 980, 991, 1463, 1692, 2510
CviQI GTAC 9 cut(s) 230, 293, 428, 616, 786, 941, 1154, 2451, 2525
DdeI CTNAG 6 cut(s) 24, 1096, 1348, 1685, 1928, 2210
DpnI GATC 7 cut(s) 995, 1467, 1922, 2156, 2215, 2233, 2504
DpnII GATC 7 cut(s) 993, 1465, 1920, 2154, 2213, 2231, 2502
DraI TTTAAA 2 cut(s) 1003, 1644
DraIII CACNNNGTG 1 cut(s) 920
DriI GACNNNNNGTC 1 cut(s) 826
Eam1104I CTCTTC 3 cut(s) 478, 1365, 2040
Eam1105I GACNNNNNGTC 1 cut(s) 826
EarI CTCTTC 3 cut(s) 478, 1365, 2040
Eco130I CCWWGG 1 cut(s) 1281
Eco31I GGTCTC 2 cut(s) 20, 2213
Eco32I GATATC 3 cut(s) 685, 775, 1790
Eco47I GGWCC 1 cut(s) 2496
Eco57I CTGAAG 3 cut(s) 119, 125, 444
Eco88I CYCGRG 2 cut(s) 1699, 2014
EcoRII CCWGG 2 cut(s) 1582, 1797
EcoRV GATATC 3 cut(s) 685, 775, 1790
EcoT14I CCWWGG 1 cut(s) 1281
EcoT22I ATGCAT 5 cut(s) 960, 1015, 1234, 1697, 2412
ErhI CCWWGG 1 cut(s) 1281
Esp3I CGTCTC 3 cut(s) 37, 1367, 2017
FaeI CATG 6 cut(s) 95, 983, 994, 1466, 1695, 2513
FaqI GGGAC 5 cut(s) 833, 1298, 1512, 1586, 2498
FatI CATG 6 cut(s) 91, 979, 990, 1462, 1691, 2509
FauI CCCGC 1 cut(s) 1837
FbaI TGATCA 1 cut(s) 1920
FblI GTMKAC 1 cut(s) 2373
Fnu4HI GCNGC 5 cut(s) 57, 1209, 1238, 1952, 2399
FokI GGATG 7 cut(s) 158, 239, 821, 869, 1336, 1670, 1814
Fsp4HI GCNGC 5 cut(s) 57, 1209, 1238, 1952, 2399
FspBI CTAG 6 cut(s) 44, 374, 1103, 1112, 1382, 1724
GluI GCNGC 5 cut(s) 57, 1209, 1238, 1952, 2399
GsaI CCCAGC 1 cut(s) 2081
GsuI CTGGAG 4 cut(s) 1601, 1605, 2123, 2375
HapII CCGG 1 cut(s) 1552
HgaI GACGC 2 cut(s) 268, 1992
Hin1I GRCGYC 1 cut(s) 1984
Hin1II CATG 6 cut(s) 95, 983, 994, 1466, 1695, 2513
HincII GTYRAC 2 cut(s) 831, 2227
HindII GTYRAC 2 cut(s) 831, 2227
HindIII AAGCTT 2 cut(s) 1823, 2171
HpaII CCGG 1 cut(s) 1552
HphI GGTGA 6 cut(s) 1981, 2054, 2164, 2171, 2338, 2377
Hpy166II GTNNAC 9 cut(s) 295, 616, 831, 935, 943, 1271, 1721, 2227, 2374
Hpy188I TCNGA 9 cut(s) 251, 260, 504, 673, 1931, 1982, 2213, 2236, 2539
Hpy8I GTNNAC 9 cut(s) 295, 616, 831, 935, 943, 1271, 1721, 2227, 2374
Hpy99I CGWCG 1 cut(s) 2534
HpyAV CCTTC 4 cut(s) 438, 962, 2078, 2189
HpyCH4III ACNGT 5 cut(s) 31, 844, 1516, 2093, 2422
HpyCH4IV ACGT 4 cut(s) 1020, 1156, 1732, 2010
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 83, 103, 1125, 2257, 2395, 2407
HpyF3I CTNAG 6 cut(s) 24, 1096, 1348, 1685, 1928, 2210
HpySE526I ACGT 4 cut(s) 1020, 1156, 1732, 2010
Hsp92I GRCGYC 1 cut(s) 1984
Hsp92II CATG 6 cut(s) 95, 983, 994, 1466, 1695, 2513
Ksp22I TGATCA 1 cut(s) 1920
Kzo9I GATC 7 cut(s) 993, 1465, 1920, 2154, 2213, 2231, 2502
LmnI GCTCC 8 cut(s) 261, 276, 884, 1030, 1116, 1128, 1750, 2356
Lsp1109I GCAGC 3 cut(s) 43, 1195, 1963
LweI GCATC 6 cut(s) 136, 208, 1648, 1704, 2208, 2459
MaeI CTAG 6 cut(s) 44, 374, 1103, 1112, 1382, 1724
MaeII ACGT 4 cut(s) 1020, 1156, 1732, 2010
MaeIII GTNAC 6 cut(s) 907, 1105, 1223, 1769, 1896, 2334
MalI GATC 7 cut(s) 995, 1467, 1922, 2156, 2215, 2233, 2504
MbiI CCGCTC 1 cut(s) 1128
MboI GATC 7 cut(s) 993, 1465, 1920, 2154, 2213, 2231, 2502
MfeI CAATTG 1 cut(s) 161
MhlI GDGCHC 2 cut(s) 88, 1723
MlyI GAGTC 8 cut(s) 255, 449, 836, 1608, 1786, 2114, 2276, 2528
MmeI TCCRAC 5 cut(s) 283, 293, 460, 862, 1602
Mph1103I ATGCAT 5 cut(s) 960, 1015, 1234, 1697, 2412
MslI CAYNNNNRTG 1 cut(s) 1202
MspA1I CMGCKG 1 cut(s) 2077
MspCI CTTAAG 1 cut(s) 1864
MspI CCGG 1 cut(s) 1552
MspR9I CCNGG 2 cut(s) 1584, 1799
MunI CAATTG 1 cut(s) 161
Mva1269I GAATGC 1 cut(s) 958
MvaI CCWGG 2 cut(s) 1584, 1799
MvnI CGCG 1 cut(s) 1146
MwoI GCNNNNNNNGC 7 cut(s) 53, 83, 103, 1125, 2257, 2395, 2407
NdeII GATC 7 cut(s) 993, 1465, 1920, 2154, 2213, 2231, 2502
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 6 cut(s) 95, 983, 994, 1466, 1695, 2513
NlaIV GGNNCC 4 cut(s) 85, 529, 886, 1916
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 1 cut(s) 907
NsiI ATGCAT 5 cut(s) 960, 1015, 1234, 1697, 2412
NspI RCATGY 2 cut(s) 1695, 2513
PaeR7I CTCGAG 2 cut(s) 1699, 2014
PagI TCATGA 2 cut(s) 979, 1462
PciI ACATGT 1 cut(s) 2509
PcsI WCGNNNNNNNCGW 1 cut(s) 2538
PctI GAATGC 1 cut(s) 958
PfeI GAWTC 6 cut(s) 169, 316, 986, 1665, 1704, 2440
PflFI GACNNNGTC 2 cut(s) 118, 1310
PkrI GCNGC 5 cut(s) 58, 1210, 1239, 1953, 2400
PleI GAGTC 8 cut(s) 255, 449, 835, 1607, 1785, 2113, 2275, 2528
PpsI GAGTC 8 cut(s) 255, 449, 835, 1607, 1785, 2113, 2275, 2528
Ppu21I YACGTR 3 cut(s) 1021, 1157, 1733
PscI ACATGT 1 cut(s) 2509
Psp6I CCWGG 2 cut(s) 1582, 1797
PspFI CCCAGC 1 cut(s) 2077
PspGI CCWGG 2 cut(s) 1582, 1797
PspN4I GGNNCC 4 cut(s) 85, 529, 886, 1916
PspPI GGNCC 1 cut(s) 2496
PsyI GACNNNGTC 2 cut(s) 118, 1310
PvuII CAGCTG 1 cut(s) 2077
RsaI GTAC 9 cut(s) 231, 294, 429, 617, 787, 942, 1155, 2452, 2526
RsaNI GTAC 9 cut(s) 230, 293, 428, 616, 786, 941, 1154, 2451, 2525
RseI CAYNNNNRTG 1 cut(s) 1202
SatI GCNGC 5 cut(s) 57, 1209, 1238, 1952, 2399
Sau3AI GATC 7 cut(s) 993, 1465, 1920, 2154, 2213, 2231, 2502
Sau96I GGNCC 1 cut(s) 2496
SchI GAGTC 8 cut(s) 255, 449, 836, 1608, 1786, 2114, 2276, 2528
ScrFI CCNGG 2 cut(s) 1584, 1799
SduI GDGCHC 2 cut(s) 88, 1723
SfaNI GCATC 6 cut(s) 136, 208, 1648, 1704, 2208, 2459
Sfr274I CTCGAG 2 cut(s) 1699, 2014
SinI GGWCC 1 cut(s) 2496
SlaI CTCGAG 2 cut(s) 1699, 2014
SmiMI CAYNNNNRTG 1 cut(s) 1202
SpeI ACTAGT 2 cut(s) 373, 1102
SsiI CCGC 5 cut(s) 1126, 1238, 1830, 2399, 2437
SspI AATATT 1 cut(s) 1679
SspMI CTAG 6 cut(s) 44, 374, 1103, 1112, 1382, 1724
StyD4I CCNGG 2 cut(s) 1582, 1797
StyI CCWWGG 1 cut(s) 1281
TaaI ACNGT 5 cut(s) 31, 844, 1516, 2093, 2422
TaiI ACGT 4 cut(s) 1023, 1159, 1735, 2013
TaqI TCGA 5 cut(s) 411, 1130, 1700, 2015, 2529
TatI WGTACW 5 cut(s) 229, 615, 940, 2450, 2524
TauI GCSGC 2 cut(s) 1240, 2401
TfiI GAWTC 6 cut(s) 169, 316, 986, 1665, 1704, 2440
TscAI CASTG 3 cut(s) 925, 1497, 2425
TseFI GTSAC 1 cut(s) 907
TseI GCWGC 3 cut(s) 56, 1208, 1951
Tsp45I GTSAC 1 cut(s) 907
TspDTI ATGAA 6 cut(s) 405, 770, 1185, 1374, 1451, 1600
TspGWI ACGGA 2 cut(s) 289, 2244
TspRI CASTG 3 cut(s) 925, 1497, 2425
Tth111I GACNNNGTC 2 cut(s) 118, 1310
Vha464I CTTAAG 1 cut(s) 1864
VneI GTGCAC 1 cut(s) 1719
VpaK11BI GGWCC 1 cut(s) 2496
XapI RAATTY 6 cut(s) 239, 641, 677, 1458, 1607, 1852
XbaI TCTAGA 1 cut(s) 1381
XceI RCATGY 2 cut(s) 1695, 2513
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XhoI CTCGAG 2 cut(s) 1699, 2014
XmiI GTMKAC 1 cut(s) 2373
XspI CTAG 6 cut(s) 44, 374, 1103, 1112, 1382, 1724
Zsp2I ATGCAT 5 cut(s) 960, 1015, 1234, 1697, 2412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.