FvH4_7g31321

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000159
Physical Location & Seq
Forward (+)
22669427 .. 22672957
3531 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31321.t1

Sequence Viewer

Length: 2607 bp
ATGGAGTTTGCCTCCGGCATCACTGACATCGTCTTGGGAAGGCTAGGCTCATATGCTGCCCAAGAAATTTGCTTGGCATGGGGCGCCCAACTTGAACTAAAGAAGCTCAATAACACCTTGTTAGCCATCAAAGTTGTGCTCCACGATGCAGAGAAGAAGCAGATGCAGACTTCCAGCATAATCCATTGGTTAGGAAACCTCAAAGATATCTGCAACGATGTTGAGGATGTACTAGACGAACTGGAGTTTCAAAAGCTACGACAAGTGCTCATCGACGAGCAGCGCAGCGTGAAAGGAAAGGTACACCAGTTCTTCTCTCGTTGGAATCCAATTGTTTTTAACTTCAAAATGGGTCATAAGATCAAAGCGATTAGAGAAAGACTAGTTGAGATTGATGACGAGAAGACAAAGTTTTCTCTCGTTCAAATTGCTGCACCAAGTGAAGAGTATCGTGTGCCCCAAAGGGTGCATGATAATGTAAGGGAGACTGACTCTTTACCGGAGGTGTATGTTGTTGGGAGAGATGAGGACAAAAAACAGATTATTACTCATCTCTTGAATGACATTGACTCCAGGAATGAAAATGTTAGTGTTATCTCCATCTTCGGGTTAGGTGGCTTGGGGAAAACTACACTTGCAAAAATGGTGTACAATGAGAGTGTGGTAGAAGAAAGCTTCAAGTTGCGGATGTGGATATGTGTCTCGGAGAACTTTGATAACAAGAGACTGGTTCGTGAGATTACGAGTGCTGCAACTAAGAGCAAATGTAAAGAAACTACTTTGGATCTTATGAAGACAAGGTTGCAAGATATATTAAAAGGTAAACAGTTTTTGTTAGTCTTGGATGATGTTTGGGATACAATTCCATTAGGAGTGACAACTCAAAAATGGAGAGAACTTAAAGCGTTGTTAAATGTTGGAGCATGTGGGAGTAAAATCATGGTAACAACACGCGATGAATCAGTTGCTTCACTTGTGCATCCTGTACATCTGCATTCTTTGGAAGGTCTTTCTCACGAGGACTCCATGGCTTTGTTTAGAAAAAATGCATTCAACGAAGGGGAGGAGCAACAGTATCCACACTTGATGAAAATCGGGGAGGACATTATGAAGAACTGTGCTGGGGTTCCCTTAGCACTAGCTACTCTAGGGAGTCTGCTCTACACAAAAAGGGAGCGACGTCAGTGGTTACAAGTAAGAGATGATGAAAGCATTCCAGGAGAGAACAACATTATGGCTGCACTAAAATTGAGCTACAATGCATTGCCGCCACACTTGAAACCTTGTTTTGCATTTTGTTCACTTTACCCCAAGGATTATGACTTTCATAGTCAAGAAATAGTTGCATTATGGATGGCACAAGGCTTCCTCAAATCATCAAGAGAAAATGAAGATTTTGATGAGTTGGGCCTAGACTATATAAGGCAGTTTTGTTCCAAGTCTTTGTTTCAACCAGAGGTAGATAGAAAGACTGAAATGCAGTTCAAGATTCATGATTTAGTGCATGATTTAGCAATAGCAGTGGCAGAAAAAGAGTGCTCCACAGTTAACAAGCGTCCCTCTAGAGAGTTCTCAAAAGTCCGGCACATGTCAATATCAGCAAAAGACTTGTCCAGAGAAGATGCAAGAGTCCCGGAGTTCATTCTCCAATTGGAGAAACTACGAACCATTCTATACATCGAACGTGGATTCTCCAATCAATATATTCTAAGGTCATGCATCTCAAGATTCAAGTACATGCGTGCACTAGATATACGTGGTTCTACTCTTGAAGAGCTACCAAGTTCCATCGGCAACTTGATTCATTTAAGATATCTCAACCTGGATGAAAACATAAAGATAAAACGCCTTCCCACTTCTATTTGCAAGCTCTTGTTTTTGCAGTTCTTAAGCCTTGAGAATTGTGTGGCACTTGAGGAGTTACCAAAAGGCATAGGGAACCTGATCAACCTCAGGTTTTTAAATATAAACACAATGCAGATGTCTTTTCCTACAGAACTATTTAGACGCCTCACCTTACTTCAAACCTTAATCATCGGGAATTGCAGAAATGTGAAATCACTGGGGGGAGATATCGAATACCTCACTAACCTTCGTCATTTGTGGATAGGTAAATGTGAGAATCTGGAGTCCTTGCCACCTAACATGAGGAACTTGAATGCATTACATACTTTCTCCATCCATGATTGTAAGAAGCTCGAGTTAATGAGGTCGGGAGAAGGCATAAAAGGTCTCATATTATTCACCATATTCACATCTGACTTGGAGGCTCTGCCAGATTGGCTTCAGGAGTCTGCAGACACTTTACAAAGCATGACAATCGCAAAGTGTGATAAACTTGCAGCACTTCCAGAGTGGTTGCAAAACCTCACGTTGCTCGAGCAACTAATGATTTGGAAATGCCCCAAATTGTCTGCTTTACCTCAAGGCATGCATTGTCTCACTGCGTTGAGAGAGTTGTATATTGTACGGTGTCCTGCATTGAGAAAAAGATGCAAAAGAGAAACAGGTGAGGATTGGTCCAAGATCAAACATGTCCCTAAGATTCATCTTGATGACTCTGATGATGAAGGTGCCAATGATATCGATGATCAGGTGCAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

869

Amino Acids

99.61

Weight (kDa)

6.57

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 89 3.1e-19 Rx N-terminal domain
NB-ARC PF00931 178 - 353 3.8e-33 NB-ARC domain
AAA_22 PF13401 197 - 310 9.1e-07 AAA domain
WHD_DRP PF23559 435 - 505 2.7e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 572 - 774 4.4e-24 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1183
AccB1I GGYRCC 2 cut(s) 83, 2573
AccII CGCG 1 cut(s) 954
AciI CCGC 2 cut(s) 685, 1268
AclWI GGATC 1 cut(s) 792
AcsI RAATTY 1 cut(s) 66
AcuI CTGAAG 1 cut(s) 2270
AcyI GRCGYC 3 cut(s) 84, 1180, 2008
AdeI CACNNNGTG 1 cut(s) 440
AfaI GTAC 6 cut(s) 231, 303, 650, 987, 1736, 2469
AfiI CCNNNNNNNGG 1 cut(s) 606
AflII CTTAAG 1 cut(s) 1888
AflIII ACRYGT 2 cut(s) 1587, 2533
AhlI ACTAGT 1 cut(s) 382
AjnI CCWGG 3 cut(s) 572, 1216, 1821
AluBI AGCT 8 cut(s) 106, 256, 675, 1142, 1254, 1777, 1870, 2197
AluI AGCT 8 cut(s) 106, 256, 675, 1142, 1254, 1777, 1870, 2197
Alw21I GWGCWC 4 cut(s) 141, 270, 1541, 1747
Alw26I GTCTC 5 cut(s) 479, 706, 718, 2237, 2444
Alw44I GTGCAC 1 cut(s) 1743
AlwI GGATC 1 cut(s) 792
Ama87I CYCGRG 2 cut(s) 2198, 2378
AoxI GGCC 1 cut(s) 1408
ApaLI GTGCAC 1 cut(s) 1743
ApeKI GCWGC 7 cut(s) 56, 280, 285, 431, 749, 1238, 2342
ApoI RAATTY 1 cut(s) 66
ArsI GACNNNNNNTTYG 2 cut(s) 1595, 1627
Asp700I GAANNNNTTC 1 cut(s) 1212
AspLEI GCGC 2 cut(s) 86, 285
AspS9I GGNCC 2 cut(s) 1408, 2520
AsuC2I CCSGG 1 cut(s) 1634
AsuHPI GGTGA 3 cut(s) 2005, 2236, 2522
AvaI CYCGRG 2 cut(s) 2198, 2378
AvaII GGWCC 1 cut(s) 2520
AxyI CCTNAGG 1 cut(s) 1952
BaeGI GKGCMC 2 cut(s) 459, 1747
BaeI ACNNNNGTAYC 2 cut(s) 293, 326
BanI GGYRCC 2 cut(s) 83, 2573
BauI CACGAG 1 cut(s) 1016
BbsI GAAGAC 2 cut(s) 410, 800
Bbv12I GWGCWC 4 cut(s) 141, 270, 1541, 1747
BbvI GCAGC 7 cut(s) 43, 292, 297, 418, 736, 1225, 2354
BccI CCATC 5 cut(s) 134, 608, 1348, 1796, 2186
BcgI CGANNNNNNTGC 2 cut(s) 2302, 2336
BciT130I CCWGG 3 cut(s) 574, 1218, 1823
BciVI GTATCC 2 cut(s) 850, 1086
BclI TGATCA 2 cut(s) 1944, 2590
BcnI CCSGG 1 cut(s) 1634
BcoDI GTCTC 5 cut(s) 479, 706, 718, 2237, 2444
BcuI ACTAGT 1 cut(s) 382
BfaI CTAG 8 cut(s) 44, 233, 383, 1139, 1148, 1412, 1563, 1748
BfmI CTRYAG 2 cut(s) 1992, 2295
BfoI RGCGCY 1 cut(s) 87
BfrI CTTAAG 1 cut(s) 1888
BfuI GTATCC 2 cut(s) 850, 1086
BglI GCCNNNNNGGC 1 cut(s) 2281
BisI GCNGC 8 cut(s) 57, 281, 286, 432, 750, 1239, 1268, 2343
BlsI GCNGC 8 cut(s) 58, 282, 287, 433, 751, 1240, 1269, 2344
Bme1390I CCNGG 4 cut(s) 574, 1218, 1634, 1823
Bme18I GGWCC 1 cut(s) 2520
BmeT110I CYCGRG 2 cut(s) 2198, 2378
BmgT120I GGNCC 2 cut(s) 1408, 2520
BmiI GGNNCC 4 cut(s) 85, 1128, 1940, 2575
BmrFI CCNGG 4 cut(s) 574, 1218, 1634, 1823
BmrI ACTGGG 1 cut(s) 2072
BmsI GCATC 7 cut(s) 27, 136, 153, 988, 1612, 1728, 2483
BmuI ACTGGG 1 cut(s) 2072
BpiI GAAGAC 2 cut(s) 410, 800
BplI GAGNNNNNCTC 3 cut(s) 28, 476, 508
BpmI CTGGAG 3 cut(s) 263, 556, 2147
Bpu10I CCTNAGC 1 cut(s) 1132
BpuEI CTTGAG 4 cut(s) 1708, 1916, 1934, 2409
BpuMI CCSGG 1 cut(s) 1634
Bsa29I ATCGAT 1 cut(s) 2586
BsaAI YACGTR 1 cut(s) 1757
BsaBI GATNNNNATC 1 cut(s) 1091
BsaHI GRCGYC 3 cut(s) 84, 1180, 2008
BsaI GGTCTC 1 cut(s) 2237
BsaJI CCNNGG 2 cut(s) 1026, 1311
BsaWI WCCGGW 1 cut(s) 499
BsaXI ACNNNNNCTCC 6 cut(s) 554, 584, 1007, 1037, 1058, 1088
Bsc4I CCNNNNNNNGG 1 cut(s) 606
Bse1I ACTGG 4 cut(s) 246, 307, 732, 2067
Bse21I CCTNAGG 1 cut(s) 1952
Bse3DI GCAATG 1 cut(s) 1262
Bse8I GATNNNNATC 1 cut(s) 1091
BseBI CCWGG 3 cut(s) 574, 1218, 1823
BseCI ATCGAT 1 cut(s) 2586
BseDI CCNNGG 2 cut(s) 1026, 1311
BseGI GGATG 7 cut(s) 232, 693, 850, 979, 1359, 1831, 2178
BseJI GATNNNNATC 1 cut(s) 1091
BseLI CCNNNNNNNGG 1 cut(s) 606
BseMI GCAATG 1 cut(s) 1262
BseMII CTCAG 1 cut(s) 1966
BseNI ACTGG 4 cut(s) 246, 307, 732, 2067
BseRI GAGGAG 2 cut(s) 1079, 1931
BseSI GKGCMC 2 cut(s) 459, 1747
BseXI GCAGC 7 cut(s) 43, 292, 297, 418, 736, 1225, 2354
BseYI CCCAGC 1 cut(s) 1121
BsgI GTGCAG 2 cut(s) 417, 1224
Bsh1236I CGCG 1 cut(s) 954
BshFI GGCC 1 cut(s) 1410
BshNI GGYRCC 2 cut(s) 83, 2573
BshVI ATCGAT 1 cut(s) 2586
BsiHKAI GWGCWC 4 cut(s) 141, 270, 1541, 1747
BsiHKCI CYCGRG 2 cut(s) 2198, 2378
BsiSI CCGG 4 cut(s) 15, 500, 1582, 1634
BslFI GGGAC 3 cut(s) 1542, 1616, 2522
BslI CCNNNNNNNGG 1 cut(s) 606
BsmAI GTCTC 5 cut(s) 479, 706, 718, 2237, 2444
BsmFI GGGAC 3 cut(s) 1542, 1616, 2522
BsmI GAATGC 4 cut(s) 994, 1049, 1212, 2164
BsnI GGCC 1 cut(s) 1410
Bso31I GGTCTC 1 cut(s) 2237
BsoBI CYCGRG 2 cut(s) 2198, 2378
Bsp1286I GDGCHC 5 cut(s) 141, 270, 459, 1541, 1747
Bsp1407I TGTACA 2 cut(s) 648, 985
Bsp143I GATC 5 cut(s) 360, 784, 1944, 2526, 2590
Bsp19I CCATGG 1 cut(s) 1026
BspACI CCGC 2 cut(s) 685, 1268
BspANI GGCC 1 cut(s) 1410
BspCNI CTCAG 1 cut(s) 1965
BspDI ATCGAT 1 cut(s) 2586
BspFNI CGCG 1 cut(s) 954
BspHI TCATGA 1 cut(s) 1492
BspLI GGNNCC 4 cut(s) 85, 1128, 1940, 2575
BspMAI CTGCAG 1 cut(s) 2299
BspPI GGATC 1 cut(s) 792
BspQI GCTCTTC 1 cut(s) 1767
BspT107I GGYRCC 2 cut(s) 83, 2573
BspTI CTTAAG 1 cut(s) 1888
BspTNI GGTCTC 1 cut(s) 2237
BsrDI GCAATG 1 cut(s) 1262
BsrGI TGTACA 2 cut(s) 648, 985
BsrI ACTGG 4 cut(s) 246, 307, 732, 2067
BssECI CCNNGG 2 cut(s) 1026, 1311
BssMI GATC 5 cut(s) 360, 784, 1944, 2526, 2590
BssNI GRCGYC 3 cut(s) 84, 1180, 2008
BssSI CACGAG 1 cut(s) 1016
BssT1I CCWWGG 2 cut(s) 1026, 1311
Bst2BI CACGAG 1 cut(s) 1016
Bst2UI CCWGG 3 cut(s) 574, 1218, 1823
Bst4CI ACNGT 5 cut(s) 828, 1074, 1118, 1546, 2472
Bst6I CTCTTC 2 cut(s) 438, 1767
BstACI GRCGYC 3 cut(s) 84, 1180, 2008
BstAFI CTTAAG 1 cut(s) 1888
BstAUI TGTACA 2 cut(s) 648, 985
BstBAI YACGTR 1 cut(s) 1757
BstC8I GCNNGC 3 cut(s) 1743, 1868, 2432
BstDEI CTNAG 5 cut(s) 756, 1132, 1709, 1952, 2541
BstDSI CCRYGG 1 cut(s) 1026
BstF5I GGATG 7 cut(s) 232, 693, 850, 979, 1359, 1831, 2178
BstFNI CGCG 1 cut(s) 954
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 2 cut(s) 86, 285
BstKTI GATC 5 cut(s) 363, 787, 1947, 2529, 2593
BstMAI GTCTC 5 cut(s) 479, 706, 718, 2237, 2444
BstMBI GATC 5 cut(s) 360, 784, 1944, 2526, 2590
BstMWI GCNNNNNNNGC 2 cut(s) 83, 2281
BstNI CCWGG 3 cut(s) 574, 1218, 1823
BstNSI RCATGY 5 cut(s) 927, 1591, 1741, 2434, 2537
BstSCI CCNGG 4 cut(s) 572, 1216, 1632, 1821
BstSFI CTRYAG 2 cut(s) 1992, 2295
BstSLI GKGCMC 2 cut(s) 459, 1747
BstUI CGCG 1 cut(s) 954
BstV1I GCAGC 7 cut(s) 43, 292, 297, 418, 736, 1225, 2354
BstV2I GAAGAC 2 cut(s) 410, 800
BstX2I RGATCY 1 cut(s) 784
BstYI RGATCY 1 cut(s) 784
Bsu15I ATCGAT 1 cut(s) 2586
Bsu36I CCTNAGG 1 cut(s) 1952
BsuI GTATCC 2 cut(s) 850, 1086
BsuRI GGCC 1 cut(s) 1410
BsuTUI ATCGAT 1 cut(s) 2586
BtgI CCRYGG 1 cut(s) 1026
BtgZI GCGATG 1 cut(s) 969
BtsCI GGATG 7 cut(s) 232, 693, 850, 979, 1359, 1831, 2178
BtsI GCAGTG 2 cut(s) 1527, 2442
BtsIMutI CAGTG 5 cut(s) 21, 1190, 1527, 2060, 2442
Cac8I GCNNGC 3 cut(s) 1743, 1868, 2432
CciI TCATGA 1 cut(s) 1492
CfoI GCGC 2 cut(s) 86, 285
Cfr13I GGNCC 2 cut(s) 1408, 2520
ClaI ATCGAT 1 cut(s) 2586
CseI GACGC 2 cut(s) 1544, 2016
Csp6I GTAC 6 cut(s) 230, 302, 649, 986, 1735, 2468
CspCI CAANNNNNGTGG 2 cut(s) 1503, 1538
CviQI GTAC 6 cut(s) 230, 302, 649, 986, 1735, 2468
DdeI CTNAG 5 cut(s) 756, 1132, 1709, 1952, 2541
DinI GGCGCC 1 cut(s) 85
DpnI GATC 5 cut(s) 362, 786, 1946, 2528, 2592
DpnII GATC 5 cut(s) 360, 784, 1944, 2526, 2590
DraI TTTAAA 1 cut(s) 1962
DraIII CACNNNGTG 1 cut(s) 440
Eam1104I CTCTTC 2 cut(s) 438, 1767
EarI CTCTTC 2 cut(s) 438, 1767
Eco130I CCWWGG 2 cut(s) 1026, 1311
Eco31I GGTCTC 1 cut(s) 2237
Eco32I GATATC 4 cut(s) 208, 1814, 2074, 2584
Eco47I GGWCC 1 cut(s) 2520
Eco57I CTGAAG 1 cut(s) 2270
Eco81I CCTNAGG 1 cut(s) 1952
Eco88I CYCGRG 2 cut(s) 2198, 2378
EcoRII CCWGG 3 cut(s) 572, 1216, 1821
EcoRV GATATC 4 cut(s) 208, 1814, 2074, 2584
EcoT14I CCWWGG 2 cut(s) 1026, 1311
EcoT22I ATGCAT 5 cut(s) 1051, 1264, 1721, 2164, 2436
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
ErhI CCWWGG 2 cut(s) 1026, 1311
FaqI GGGAC 3 cut(s) 1542, 1616, 2522
FauNDI CATATG 1 cut(s) 52
FbaI TGATCA 2 cut(s) 1944, 2590
Fnu4HI GCNGC 8 cut(s) 57, 281, 286, 432, 750, 1239, 1268, 2343
FokI GGATG 7 cut(s) 239, 700, 857, 966, 1366, 1838, 2165
Fsp4HI GCNGC 8 cut(s) 57, 281, 286, 432, 750, 1239, 1268, 2343
FspBI CTAG 8 cut(s) 44, 233, 383, 1139, 1148, 1412, 1563, 1748
GlaI GCGC 2 cut(s) 85, 284
GluI GCNGC 8 cut(s) 57, 281, 286, 432, 750, 1239, 1268, 2343
GsaI CCCAGC 1 cut(s) 1125
GsuI CTGGAG 3 cut(s) 263, 556, 2147
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 1 cut(s) 1410
HapII CCGG 4 cut(s) 15, 500, 1582, 1634
HgaI GACGC 2 cut(s) 1544, 2016
HhaI GCGC 2 cut(s) 86, 285
Hin1I GRCGYC 3 cut(s) 84, 1180, 2008
Hin6I GCGC 2 cut(s) 84, 283
HinP1I GCGC 2 cut(s) 84, 283
HincII GTYRAC 1 cut(s) 1549
HindII GTYRAC 1 cut(s) 1549
HindIII AAGCTT 1 cut(s) 673
HpaI GTTAAC 1 cut(s) 1549
HpaII CCGG 4 cut(s) 15, 500, 1582, 1634
HphI GGTGA 3 cut(s) 2005, 2236, 2522
Hpy166II GTNNAC 6 cut(s) 304, 649, 824, 1301, 1549, 1745
Hpy188I TCNGA 3 cut(s) 706, 2260, 2563
Hpy8I GTNNAC 6 cut(s) 304, 649, 824, 1301, 1549, 1745
Hpy99I CGWCG 2 cut(s) 278, 1182
HpyAV CCTTC 7 cut(s) 33, 998, 1052, 1859, 2102, 2213, 2564
HpyCH4III ACNGT 5 cut(s) 828, 1074, 1118, 1546, 2472
HpyCH4IV ACGT 4 cut(s) 1180, 1684, 1756, 2372
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 2281
HpyF3I CTNAG 5 cut(s) 756, 1132, 1709, 1952, 2541
HpySE526I ACGT 4 cut(s) 1180, 1684, 1756, 2372
Hsp92I GRCGYC 3 cut(s) 84, 1180, 2008
HspAI GCGC 2 cut(s) 84, 283
KasI GGCGCC 1 cut(s) 83
Ksp22I TGATCA 2 cut(s) 1944, 2590
KspAI GTTAAC 1 cut(s) 1549
Kzo9I GATC 5 cut(s) 360, 784, 1944, 2526, 2590
LguI GCTCTTC 1 cut(s) 1767
LmnI GCTCC 5 cut(s) 144, 920, 1066, 1174, 1544
Lsp1109I GCAGC 7 cut(s) 43, 292, 297, 418, 736, 1225, 2354
LweI GCATC 7 cut(s) 27, 136, 153, 988, 1612, 1728, 2483
MaeI CTAG 8 cut(s) 44, 233, 383, 1139, 1148, 1412, 1563, 1748
MaeII ACGT 4 cut(s) 1180, 1684, 1756, 2372
MaeIII GTNAC 4 cut(s) 874, 943, 1188, 1920
MalI GATC 5 cut(s) 362, 786, 1946, 2528, 2592
MboI GATC 5 cut(s) 360, 784, 1944, 2526, 2590
MfeI CAATTG 2 cut(s) 330, 1649
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 5 cut(s) 141, 270, 459, 1541, 1747
MluCI AATT 9 cut(s) 66, 330, 426, 861, 1247, 1649, 1900, 2041, 2408
Mly113I GGCGCC 1 cut(s) 84
MlyI GAGTC 8 cut(s) 485, 563, 1016, 1162, 1638, 2138, 2300, 2552
MmeI TCCRAC 2 cut(s) 302, 898
Mph1103I ATGCAT 5 cut(s) 1051, 1264, 1721, 2164, 2436
MroXI GAANNNNTTC 1 cut(s) 1212
MslI CAYNNNNRTG 2 cut(s) 474, 2553
MspCI CTTAAG 1 cut(s) 1888
MspI CCGG 4 cut(s) 15, 500, 1582, 1634
MspR9I CCNGG 4 cut(s) 574, 1218, 1634, 1823
MunI CAATTG 2 cut(s) 330, 1649
Mva1269I GAATGC 4 cut(s) 994, 1049, 1212, 2164
MvaI CCWGG 3 cut(s) 574, 1218, 1823
MvnI CGCG 1 cut(s) 954
MwoI GCNNNNNNNGC 2 cut(s) 83, 2281
NarI GGCGCC 1 cut(s) 84
NciI CCSGG 1 cut(s) 1634
NcoI CCATGG 1 cut(s) 1026
NdeI CATATG 1 cut(s) 52
NdeII GATC 5 cut(s) 360, 784, 1944, 2526, 2590
NlaIV GGNNCC 4 cut(s) 85, 1128, 1940, 2575
NmuCI GTSAC 1 cut(s) 874
NsiI ATGCAT 5 cut(s) 1051, 1264, 1721, 2164, 2436
NspI RCATGY 5 cut(s) 927, 1591, 1741, 2434, 2537
PaeI GCATGC 1 cut(s) 2434
PaeR7I CTCGAG 2 cut(s) 2198, 2378
PagI TCATGA 1 cut(s) 1492
PciI ACATGT 2 cut(s) 1587, 2533
PciSI GCTCTTC 1 cut(s) 1767
PctI GAATGC 4 cut(s) 994, 1049, 1212, 2164
PdmI GAANNNNTTC 1 cut(s) 1212
PfeI GAWTC 8 cut(s) 325, 959, 1489, 1689, 1728, 1801, 2122, 2545
PflFI GACNNNGTC 1 cut(s) 29
PfoI TCCNGGA 3 cut(s) 572, 1216, 1632
PkrI GCNGC 8 cut(s) 58, 282, 287, 433, 751, 1240, 1269, 2344
PleI GAGTC 8 cut(s) 485, 563, 1016, 1161, 1637, 2137, 2299, 2552
PluTI GGCGCC 1 cut(s) 87
PpsI GAGTC 8 cut(s) 485, 563, 1016, 1161, 1637, 2137, 2299, 2552
Ppu21I YACGTR 1 cut(s) 1757
PscI ACATGT 2 cut(s) 1587, 2533
Psp6I CCWGG 3 cut(s) 572, 1216, 1821
PspFI CCCAGC 1 cut(s) 1121
PspGI CCWGG 3 cut(s) 572, 1216, 1821
PspN4I GGNNCC 4 cut(s) 85, 1128, 1940, 2575
PspPI GGNCC 2 cut(s) 1408, 2520
PspXI VCTCGAGB 2 cut(s) 2198, 2378
PstI CTGCAG 1 cut(s) 2299
PsuI RGATCY 1 cut(s) 784
PsyI GACNNNGTC 1 cut(s) 29
RsaI GTAC 6 cut(s) 231, 303, 650, 987, 1736, 2469
RsaNI GTAC 6 cut(s) 230, 302, 649, 986, 1735, 2468
RseI CAYNNNNRTG 2 cut(s) 474, 2553
SapI GCTCTTC 1 cut(s) 1767
SatI GCNGC 8 cut(s) 57, 281, 286, 432, 750, 1239, 1268, 2343
Sau3AI GATC 5 cut(s) 360, 784, 1944, 2526, 2590
Sau96I GGNCC 2 cut(s) 1408, 2520
SchI GAGTC 8 cut(s) 485, 563, 1016, 1162, 1638, 2138, 2300, 2552
ScrFI CCNGG 4 cut(s) 574, 1218, 1634, 1823
SduI GDGCHC 5 cut(s) 141, 270, 459, 1541, 1747
SfaNI GCATC 7 cut(s) 27, 136, 153, 988, 1612, 1728, 2483
SfcI CTRYAG 2 cut(s) 1992, 2295
SfoI GGCGCC 1 cut(s) 85
Sfr274I CTCGAG 2 cut(s) 2198, 2378
SinI GGWCC 1 cut(s) 2520
SlaI CTCGAG 2 cut(s) 2198, 2378
SmiMI CAYNNNNRTG 2 cut(s) 474, 2553
SmlI CTYRAG 7 cut(s) 1723, 1888, 1895, 1913, 2198, 2378, 2424
SmoI CTYRAG 7 cut(s) 1723, 1888, 1895, 1913, 2198, 2378, 2424
SpeI ACTAGT 1 cut(s) 382
SphI GCATGC 1 cut(s) 2434
Sse9I AATT 9 cut(s) 66, 330, 426, 861, 1247, 1649, 1900, 2041, 2408
SsiI CCGC 2 cut(s) 685, 1268
SspDI GGCGCC 1 cut(s) 83
SspMI CTAG 8 cut(s) 44, 233, 383, 1139, 1148, 1412, 1563, 1748
StyD4I CCNGG 4 cut(s) 572, 1216, 1632, 1821
StyI CCWWGG 2 cut(s) 1026, 1311
TaaI ACNGT 5 cut(s) 828, 1074, 1118, 1546, 2472
TaiI ACGT 4 cut(s) 1183, 1687, 1759, 2375
TaqI TCGA 6 cut(s) 273, 1680, 2076, 2199, 2379, 2586
TasI AATT 9 cut(s) 66, 330, 426, 861, 1247, 1649, 1900, 2041, 2408
TatI WGTACW 4 cut(s) 229, 648, 985, 1734
TauI GCSGC 1 cut(s) 1270
TfiI GAWTC 8 cut(s) 325, 959, 1489, 1689, 1728, 1801, 2122, 2545
TscAI CASTG 5 cut(s) 28, 1190, 1527, 2067, 2449
TseFI GTSAC 1 cut(s) 874
TseI GCWGC 7 cut(s) 56, 280, 285, 431, 749, 1238, 2342
Tsp45I GTSAC 1 cut(s) 874
TspRI CASTG 5 cut(s) 28, 1190, 1527, 2067, 2449
Tth111I GACNNNGTC 1 cut(s) 29
Vha464I CTTAAG 1 cut(s) 1888
VneI GTGCAC 1 cut(s) 1743
VpaK11BI GGWCC 1 cut(s) 2520
XapI RAATTY 1 cut(s) 66
XbaI TCTAGA 1 cut(s) 1562
XceI RCATGY 5 cut(s) 927, 1591, 1741, 2434, 2537
XhoI CTCGAG 2 cut(s) 2198, 2378
XmnI GAANNNNTTC 1 cut(s) 1212
XspI CTAG 8 cut(s) 44, 233, 383, 1139, 1148, 1412, 1563, 1748
ZraI GACGTC 1 cut(s) 1181
Zsp2I ATGCAT 5 cut(s) 1051, 1264, 1721, 2164, 2436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.