MD17G1284300.v1.1

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000116
Physical Location & Seq
Forward (+)
34351031 .. 34357491
6461 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1284300.v1.1.491

Sequence Viewer

Length: 3888 bp
ATGGCCGAGGAAAATGGCCTGGGTGGCGCTGTACCTCAAGAGCGTGAGGATACCACTTCAGGAAACCAAGTAGAAGAGAAAAACACCGGCACGGACGGGGCTCATCGAGGCTCAGACAAGAGCGGCGGAAATGAGAAAGTAGAAAAAGTTGCATTTTCAAAACTATTCTCATTTGCAGATAAGACTGATATTATCTTGATGCTCGTTGGCACAATCGGTGCCATTGGGAATGGCTTGTGCATGCCCCTTATGACCATATTATTTGGGGACTTGGTCAATGCCTTTGGAAATAATCAAAACAATTCAGATGTAGTTAGTGTTGTTTCCAAGGTCTCTCTAAAATTTGTGTATTTGGCGATCGGGGCAGGTGTGGCCGCAACTCTTCAGGTCTCTTGCTGGATGGTAACAGGGGAAAGACAAGCTGCAAGGATAAGGGGTTTGTATTTGGAAACAATTTTGAGACAAGACGTTGCCTTCTTTGATTTGGAAACAAACACTGGAGAGGTCATTGGGAGAATGTCTGGAGACACAGTTCTCATACAAGATGCCATGGGCGAGAAGGTTGGAAAATTTTTACAACTACTTTCAACATTCATAGGAGGGTTTATAATAGCATTTATCAAGGGGTGGCTTCTTACCGTTGTCATGTTATCCTCGATTCCTCTGCTCGTGGCAGCTGGTGCAACTATGTCAATCGTTATAACCAAGATGGCATCCCATGGACAAACTGCTTATGCAAAAGCTGCAAATGTTGTTGAACAGACGATAGGCTCCATCAGAACCGTTGCATCCTTTACTGGAGAGAAGCAAGCTATAACAAGTTACAAAAAATATCTTGTGGATGCTTACAAATCAAGCGTTTATGAAGGTACTGCTGCTGGAGCAGGTTTTGGCATCGTTATGCTAGTTGTGTTCAGCACTTATGCCTTGGCAATATGGTTTGGTTCAAGAATGGTAAGGAATAATGGATATTCTGGGGGTGCCGTGCTGAATGTGATTGTTGCTGTTTTGACTGGATCCATGTCTCTGGGGCAAGCATCACCGTGCTTGAGTGCATTCGCTGCTGGTCAAGCTGCAGCATTTAAGATGTTTGAGACTATCAGTAGGAAACCAGAGATTGATGCTTCTGACGAAAAAGGAAAGATTTTGGATGACATTCGTGGAGATATAGAGCTGAGAGATGTTTATTTCAGTTATCCTGCCAGACCAAATGAACCAATATTTGACGGGTTCTCTCTTCATATCTCTAGTGGCACCACTGCGGCTTTGGTTGGACAAAGTGGAAGTGGGAAGTCAACAGTCATCAGTCTGATGGAGAGATTTTATGATCCACGAGCTGGTGAAGTTTTGATAGATGGCATAAACCTAAAAGAATTTCAGGTGAAATGGATTCGGAGTAAAATTGGTCTTGTCAGCCAGGAACCTGTACTGTTTGCATCCAGCATTAAGGACAATATTGCCTATGGAAAGGATGGTGCTACCATTGAAGAGATAAAAGCAGCAGCTGAACTTGCAAACGCCTCCAAATTTATTGATAAATTGCCTCAGGGAGTTGACACCATGGTCGGTGATCATGGAACTCAGCTATCTGGTGGGCAGAAGCAGAGAATTGCTATAGCAAGAGCAATTTTGAAAGATCCACGAATTTTACTTTTGGATGAAGCTACAAGTGCACTTGATGCAGAATCTGAGAGGATAGTTCAAGAAGCATTGGATCGGATTATGGTCAACAGAACTACTGTTATTGTTGCTCATCGTTTGAGCACAGTGAGGAACGCAGATACGATTGCTGTCATTCATAAGGGAAAGCTGGTTGAAAAAGGCTCGCACTCAGACTTACTCAAGGATCCTGAAGGAGCATACTCTCAACTTGTACGCTTGCAAGAAGTAAACAAGGGCTCAGAACAAATAGGAGAAGCTCAAGACAAATCTGATATAACTACCGAATCTTTTAGACAGTCGAGTCAAAGAGTGTCGTTAGCAAGATCCGTAAGTCGGAATTCCTCTTTAGGAAATAGCAGCCGACATTCATTTTCAGTCGCCTTTGGTTTACCCACAGGACTTGGTAGCAATCGTGACACTACATTGGCAGAACCAGAGGCCGCTGCCCTGGCATCAAAGCCACGTCCAAAGGTCTCTCTCCGCCGCCTCGCTACCCTCAACAAGCCAGAGATTCCAGTGCTTCTTCTTGGAAGCTTAGCTGCCATCGCTAATGGTGTCATATTTCCCGTTTTTGGTGTGCTTATTTCCAGGGTAATAAAGACTTTCTACGAACCACCTCGTCAACAAAAGAAAGATTCAGAGTTTTGGGCAATAATGTTTATGACCCTTGGTCTGATATCATTTCTGGTAATCCCAGTACGAGGATACTTCTTTTCGGTTGCTGGTAGTAAGTTAATTGAACGTATCAGATTGATGTGTTTTGAGAGAGTGGTTCACATGGAAGTTGGGTGGTTTGATGAGCCTGAGAACTCAAGTGGTTCAGTTGGTGCGAGGCTCTCAGCAGATGCAGCATCAGTGCGGGCCCTAGTTGGAGATGCGCTAGCTGAGATAGTTAATAGCATTGCGACCGGAATTGCAGGTTTGATCATTGCTTTTATTGCATCCTGGCAGTTGGCATTCATTGTTCTCGCATTGATTCCTCTGATCGGATTTGGTGCTTATGTTCAAACAAAGTTCATGAAAGGATTCAGTGGAGATGCAAAGATGATGTATGAGGAAGCAAGCCAAGTTGCTAATGACGCAGTTGGGAGCATACGAACAGTTGCTTCTTTCTGTGCTGAAAAGAAGGTAATGGAACTGTACAGAAGGAAATGTGAAGGCCCAAGAGCGGAAGGGAAAAGACAAGGCGTGATCAGTGGAATAGGATTTGGGGTATCTTACTTCTTTCTCTTTTGTGTCTACGCAACCAGCTTCTACGCGGGAGCTAAACTCGTCGAGGCTGGCAAAACAAAATTTGCCGACGTTTTCCAAGTTTTCTTTGCTTTGACCATGGCAGCCACGGGAATCTCTAATATGAGCAGTTTTGGCTCTGATTCTAGTAAAGCCAGGAATGCTGCTGCTTCCATATTTGCAATAATAGACAGGAAGTCAAAGATAGACCCTAGTGACGAGTCCGGCATAAAATTGGATAGCGTAAAGGGAGATATTGAGCTTCGCCATGTAAGCTTTAAATATCCCTCTCGGCCAGATATACAGATTTTCCAAGACCTCAGTTTAACTATCCATTCTGGCAAGACAGTTGCCCTGGTTGGAGAAAGTGGGAGTGGAAAATCGACAGTAGTAGCATTGATGCAACGATTTTATGATCCGGATTCAGGTCATATCACACTTGATGGAACTGAACTTGGAAAATTCCACTTGAAATGGTTGAGGCAGCAGATGGGGCTTGTGGGGCAAGAACCTGTTTTATTTAACGACACAATCCGTGCCAACATTGCGTATGGAAAGGATGGTGATGCAACTGAGGCAGAAATCATAGCTGCATCGGAGTTGGCCAATGCACACAAATTCATTAGTAGCTTGAATCAGGGGTATGACACCGTAGTAGGAGAACGAGGAATACAATTATCTGGCGGGCAGAAGCAACGCGTAGCCATTGCGCGTGCGATAATCAAGAGCCCCAAGATATTACTGTTGGATGAAGCTACAAGTGCACTTGATTCTGAATCTGAGAGAGTGGTTCAAGATGCATTAGACAGGGTGATGGTAAACCGGACCACCGTAGTTGTTGCTCATCGACTATCGACAATCAAGAATGCAGATGAGATCGCGGTGGTTAAAAACGGAGTGATCGTAGAAAAAGGCAAGCATGATAAGTTGATCAAAATTACAGATGGATTTTATGCATCCTTGGTCGCTCTGCACATGAGCGCTCCAAATGCATGA

Protein Analysis

1296

Amino Acids

139.18

Weight (kDa)

8.13

Isoelectric Point (pI)

30.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 67 - 341 6.6e-56 ABC transporter transmembrane region
ABC_tran PF00005 409 - 557 6e-32 ABC transporter
ABC_membrane PF00664 729 - 999 7.1e-56 ABC transporter transmembrane region
ABC_tran PF00005 1069 - 1218 1.4e-34 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02520 AT1G02520 AT1G02520 AT1G02530 AT1G02530 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT3G62150 AT3G62150 AT3G62150 AT4G01820 AT4G01820 AT4G01830 AT4G01830
fragaria_vesca FvH4_1g26280 FvH4_4g21370 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21381 FvH4_4g21600 FvH4_4g21600 FvH4_4g21600 FvH4_4g21620 FvH4_4g21621 FvH4_4g21622 FvH4_6g00641 FvH4_6g00650
malus_domestica MD09G1290100.v1.1 MD13G1138600.v1.1 MD13G1138700.v1.1 MD13G1139100.v1.1 MD16G1134500.v1.1 MD16G1134700.v1.1 MD16G1135000.v1.1 MD16G1135100.v1.1 MD17G1284300.v1.1 MD17G1284700.v1.1
prunus_persica Prupe.1G103800_v2.0.a1 Prupe.1G103800_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1
pyrus_communis pycom04g08750 pycom09g19770 pycom09g19790 pycom13g09140 pycom16g11900 pycom16g11920 pycom16g11930 pycom17g28030
rosa_chinensis RchiOBHm_Chr2g0157341 RchiOBHm_Chr3g0448061 RchiOBHm_Chr3g0485931 RchiOBHm_Chr3g0485961 RchiOBHm_Chr4g0427151 RchiOBHm_Chr4g0427161 RchiOBHm_Chr4g0427211 RchiOBHm_Chr4g0427241 RchiOBHm_Chr4g0427271 RchiOBHm_Chr4g0427711 RchiOBHm_Chr4g0427721 RchiOBHm_Chr4g0427771 RchiOBHm_Chr4g0427781 RchiOBHm_Chr4g0427791 RchiOBHm_Chr4g0427851 RchiOBHm_Chr4g0427861 RchiOBHm_Chr4g0446561 RchiOBHm_Chr5g0081601 RchiOBHm_Chr6g0278251 RchiOBHm_Chr6g0303241
rosa_laevigata RLG00000007206 RLG00000007207 RLG00000007209 RLG00000007210 RLG00000007211 RLG00000007212 RLG00000007214 RLG00000007255 RLG00000007256 RLG00000007257 RLG00000011097 RLG00000019257 RLG00000023121 RLG00000025948 RLG00000027246 RLG00000029989
rosa_multiflora Rmu_co8076196.1_g000001 Rmu_co8517461.1_g000001 Rmu_sc0000066.1_g000018 Rmu_sc0000066.1_g000024 Rmu_sc0000066.1_g000033 Rmu_sc0000066.1_g000047 Rmu_sc0000066.1_g000048 Rmu_sc0001651.1_g000024 Rmu_sc0002816.1_g000003 Rmu_sc0003337.1_g000015 Rmu_sc0003444.1_g000015 Rmu_sc0003813.1_g000004 Rmu_sc0004876.1_g000001 Rmu_sc0005969.1_g000025 Rmu_sc0008650.1_g000006 Rmu_sc0008855.1_g000001 Rmu_sc0009165.1_g000001 Rmu_sc0009165.1_g000002 Rmu_sc0010010.1_g000001 Rmu_sc0010010.1_g000005 Rmu_sc0036078.1_g000001
rosa_roxburghii Rroxscaffold_2G00130200 Rroxscaffold_5G00367590 Rroxscaffold_5G00368970 Rroxscaffold_5G00368990 Rroxscaffold_5G00369340 Rroxscaffold_5G00369350 Rroxscaffold_5G00369380 Rroxscaffold_5G00369390 Rroxscaffold_5G00369440 Rroxscaffold_5G00369450 Rroxscaffold_5G00369500 Rroxscaffold_5G00369510 Rroxscaffold_6G00396190 Rroxscaffold_6G00426220 Rroxscaffold_6G00427790 Rroxscaffold_7G00164940
rosa_rugosa Rorug01G0160800.1 Rorug02G0341400 Rorug02G0461800 Rorug02G0608000 Rorug03G0222700 Rorug03G0222800 Rorug03G0222900 Rorug04G0216400 Rorug04G0216400 Rorug04G0220100 Rorug04G0220100 Rorug04G0220100 Rorug05G0181800 Rorug05G0409700
rosa_samantha Rh1BG177300 Rh1DG206800 Rh2AG590600 Rh2BG473400 Rh2DG482300 Rh3AG007500 Rh3AG272400 Rh3BG007200 Rh3BG307000 Rh3DG301600 Rh3DG301900 Rh4AG272300 Rh4AG272400 Rh4AG272700 Rh4AG275900 Rh4AG276100 Rh4AG276200 Rh4AG276500 Rh4AG276600 Rh4AG276700 Rh4AG277000 Rh4BG281900 Rh4BG282200 Rh4CG292900 Rh4CG293300 Rh4CG293400 Rh4CG293600 Rh4CG297400 Rh4CG297500 Rh4CG297900 Rh4CG298000 Rh4CG298300 Rh4CG298400 Rh4DG165900 Rh5BG011400 Rh5DG092900 Rh6AG434000 Rh6CG153300 Rh6CG391700 Rh7BG333600
rosa_wichuraiana Rw0G005570 Rw0G005590 Rw0G011850 Rw0G015530 Rw0G017350 Rw0G019580 Rw3G000600 Rw3G024160 Rw4G023620 Rw4G023640 Rw4G023660 Rw4G023670 Rw4G023950 Rw4G023960 Rw4G024050 Rw4G024060 Rw5G048020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 608, 701
AarI CACCTGC 1 cut(s) 356
AasI GACNNNNNNGTC 2 cut(s) 1562, 1962
Acc36I ACCTGC 3 cut(s) 356, 875, 2570
AccB1I GGYRCC 3 cut(s) 218, 980, 1253
AccB7I CCANNNNNTGG 1 cut(s) 1337
AccBSI CCGCTC 2 cut(s) 123, 2831
AccI GTMKAC 1 cut(s) 2901
AccII CGCG 4 cut(s) 2921, 3591, 3604, 3773
AccIII TCCGGA 1 cut(s) 3310
AclWI GGATC 9 cut(s) 1011, 1024, 1322, 1631, 1722, 1841, 1854, 1980, 3302
AcoI YGGCCR 4 cut(s) 3, 372, 3185, 3495
AcsI RAATTY 9 cut(s) 341, 569, 1373, 1526, 1644, 1999, 2954, 3353, 3509
AcuI CTGAAG 3 cut(s) 42, 368, 1872
AfaI GTAC 6 cut(s) 33, 871, 1428, 1875, 2361, 2804
AfeI AGCGCT 1 cut(s) 3874
AfiI CCNNNNNNNGG 7 cut(s) 1337, 1995, 2234, 2363, 2648, 2830, 3317
AflIII ACRYGT 1 cut(s) 3589
AhdI GACNNNNNGTC 2 cut(s) 2331, 3088
AjiI CACGTC 1 cut(s) 2126
AjnI CCWGG 7 cut(s) 18, 1416, 2109, 2249, 2606, 3047, 3246
AloI GAACNNNNNNTCC 2 cut(s) 516, 548
Alw21I GWGCWC 3 cut(s) 1675, 1766, 3658
Alw26I GTCTC 7 cut(s) 337, 394, 454, 519, 1029, 1088, 2140
Alw44I GTGCAC 2 cut(s) 1671, 3654
AlwI GGATC 9 cut(s) 1011, 1024, 1322, 1631, 1722, 1841, 1854, 1980, 3302
AlwNI CAGNNNCTG 2 cut(s) 1505, 1688
Aor13HI TCCGGA 1 cut(s) 3310
Aor51HI AGCGCT 1 cut(s) 3874
AoxI GGCC 8 cut(s) 3, 16, 372, 2100, 2523, 2821, 3185, 3495
ApaI GGGCCC 1 cut(s) 2527
ApaLI GTGCAC 2 cut(s) 1671, 3654
ApoI RAATTY 9 cut(s) 341, 569, 1373, 1526, 1644, 1999, 2954, 3353, 3509
Asp700I GAANNNNTTC 1 cut(s) 2687
AspLEI GCGC 4 cut(s) 29, 2542, 3604, 3875
AspS9I GGNCC 4 cut(s) 2523, 2524, 2822, 3717
AsuHPI GGTGA 6 cut(s) 1032, 1352, 1393, 1580, 3467, 3715
AsuNHI GCTAGC 1 cut(s) 2542
AvaII GGWCC 1 cut(s) 3717
AxyI CCTNAGG 1 cut(s) 1545
BaeGI GKGCMC 3 cut(s) 1675, 2527, 3658
BalI TGGCCA 1 cut(s) 3497
BamHI GGATCC 2 cut(s) 1016, 1846
BanI GGYRCC 3 cut(s) 218, 980, 1253
BanII GRGCYC 4 cut(s) 103, 1901, 2527, 3623
BauI CACGAG 2 cut(s) 668, 1332
Bbv12I GWGCWC 3 cut(s) 1675, 1766, 3658
BceAI ACGGC 1 cut(s) 968
BcgI CGANNNNNNTGC 2 cut(s) 646, 680
BciT130I CCWGG 7 cut(s) 20, 1418, 2111, 2251, 2608, 3049, 3248
BciVI GTATCC 2 cut(s) 43, 2360
BclI TGATCA 4 cut(s) 1570, 2586, 2853, 3822
BcoDI GTCTC 7 cut(s) 337, 394, 454, 519, 1029, 1088, 2140
BfaI CTAG 6 cut(s) 905, 1248, 2528, 2543, 3039, 3105
BfmI CTRYAG 2 cut(s) 1074, 1614
BfoI RGCGCY 2 cut(s) 30, 3876
BfuAI ACCTGC 3 cut(s) 356, 875, 2570
BfuI GTATCC 2 cut(s) 43, 2360
BglI GCCNNNNNGGC 1 cut(s) 24
BlpI GCTNAGC 1 cut(s) 2197
Bme1390I CCNGG 7 cut(s) 20, 1418, 2111, 2251, 2608, 3049, 3248
Bme18I GGWCC 1 cut(s) 3717
BmeRI GACNNNNNGTC 2 cut(s) 2331, 3088
BmgBI CACGTC 1 cut(s) 2126
BmgT120I GGNCC 4 cut(s) 2523, 2524, 2822, 3717
BmiI GGNNCC 8 cut(s) 220, 772, 982, 1018, 1255, 1422, 1848, 2525
BmrFI CCNGG 7 cut(s) 20, 1418, 2111, 2251, 2608, 3049, 3248
BmrI ACTGGG 1 cut(s) 2351
BmtI GCTAGC 1 cut(s) 2546
BmuI ACTGGG 1 cut(s) 2351
BplI GAGNNNNNCTC 2 cut(s) 2916, 2948
BpmI CTGGAG 4 cut(s) 519, 543, 819, 900
Bpu1102I GCTNAGC 1 cut(s) 2197
BpuEI CTTGAG 5 cut(s) 21, 1069, 1826, 1905, 2458
BsaI GGTCTC 3 cut(s) 337, 394, 2140
BsaWI WCCGGW 3 cut(s) 2570, 3310, 3714
BsaXI ACNNNNNCTCC 4 cut(s) 516, 546, 3780, 3810
Bsc4I CCNNNNNNNGG 7 cut(s) 1337, 1995, 2234, 2363, 2648, 2830, 3317
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 5 cut(s) 502, 802, 1018, 2177, 2357
Bse21I CCTNAGG 1 cut(s) 1545
Bse3DI GCAATG 4 cut(s) 2562, 2589, 3435, 3597
BseAI TCCGGA 1 cut(s) 3310
BseBI CCWGG 7 cut(s) 20, 1418, 2111, 2251, 2608, 3049, 3248
BseLI CCNNNNNNNGG 7 cut(s) 1337, 1995, 2234, 2363, 2648, 2830, 3317
BseMI GCAATG 4 cut(s) 2562, 2589, 3435, 3597
BseNI ACTGG 5 cut(s) 502, 802, 1018, 2177, 2357
BseSI GKGCMC 3 cut(s) 1675, 2527, 3658
BsgI GTGCAG 1 cut(s) 3848
Bsh1236I CGCG 4 cut(s) 2921, 3591, 3604, 3773
Bsh1285I CGRYCG 2 cut(s) 360, 2571
BshFI GGCC 8 cut(s) 5, 18, 374, 2102, 2525, 2823, 3187, 3497
BshNI GGYRCC 3 cut(s) 218, 980, 1253
BsiEI CGRYCG 2 cut(s) 360, 2571
BsiHKAI GWGCWC 3 cut(s) 1675, 1766, 3658
BsiSI CCGG 5 cut(s) 87, 2571, 3117, 3311, 3715
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 7 cut(s) 1337, 1995, 2234, 2363, 2648, 2830, 3317
BsmAI GTCTC 7 cut(s) 337, 394, 454, 519, 1029, 1088, 2140
BsmFI GGGAC 1 cut(s) 281
BsmI GAATGC 4 cut(s) 1055, 2618, 3058, 3763
BsnI GGCC 8 cut(s) 5, 18, 374, 2102, 2525, 2823, 3187, 3497
Bso31I GGTCTC 3 cut(s) 337, 394, 2140
Bsp120I GGGCCC 1 cut(s) 2523
Bsp1286I GDGCHC 7 cut(s) 103, 1675, 1766, 1901, 2527, 3623, 3658
Bsp13I TCCGGA 1 cut(s) 3310
Bsp1407I TGTACA 1 cut(s) 2802
Bsp1720I GCTNAGC 1 cut(s) 2197
Bsp19I CCATGG 4 cut(s) 549, 718, 1560, 2991
BspANI GGCC 8 cut(s) 5, 18, 374, 2102, 2525, 2823, 3187, 3497
BspEI TCCGGA 1 cut(s) 3310
BspFNI CGCG 4 cut(s) 2921, 3591, 3604, 3773
BspHI TCATGA 1 cut(s) 2679
BspLI GGNNCC 8 cut(s) 220, 772, 982, 1018, 1255, 1422, 1848, 2525
BspMAI CTGCAG 1 cut(s) 1078
BspMI ACCTGC 3 cut(s) 356, 875, 2570
BspOI GCTAGC 1 cut(s) 2546
BspPI GGATC 9 cut(s) 1011, 1024, 1322, 1631, 1722, 1841, 1854, 1980, 3302
BspT107I GGYRCC 3 cut(s) 218, 980, 1253
BspTNI GGTCTC 3 cut(s) 337, 394, 2140
BsrBI CCGCTC 2 cut(s) 123, 2831
BsrDI GCAATG 4 cut(s) 2562, 2589, 3435, 3597
BsrFI RCCGGY 1 cut(s) 86
BsrGI TGTACA 1 cut(s) 2802
BsrI ACTGG 5 cut(s) 502, 802, 1018, 2177, 2357
BssAI RCCGGY 1 cut(s) 86
BssSI CACGAG 2 cut(s) 668, 1332
BssT1I CCWWGG 8 cut(s) 327, 549, 718, 927, 1560, 2329, 2991, 3852
Bst2BI CACGAG 2 cut(s) 668, 1332
Bst2UI CCWGG 7 cut(s) 20, 1418, 2111, 2251, 2608, 3049, 3248
Bst6I CTCTTC 4 cut(s) 69, 387, 1242, 1482
BstAPI GCANNNNNTGC 4 cut(s) 680, 743, 1061, 1679
BstAUI TGTACA 1 cut(s) 2802
BstDSI CCRYGG 5 cut(s) 549, 718, 1560, 2991, 3000
BstFNI CGCG 4 cut(s) 2921, 3591, 3604, 3773
BstH2I RGCGCY 2 cut(s) 30, 3876
BstHHI GCGC 4 cut(s) 29, 2542, 3604, 3875
BstMAI GTCTC 7 cut(s) 337, 394, 454, 519, 1029, 1088, 2140
BstMCI CGRYCG 2 cut(s) 360, 2571
BstNI CCWGG 7 cut(s) 20, 1418, 2111, 2251, 2608, 3049, 3248
BstNSI RCATGY 1 cut(s) 244
BstSCI CCNGG 7 cut(s) 18, 1416, 2109, 2249, 2606, 3047, 3246
BstSFI CTRYAG 2 cut(s) 1074, 1614
BstSLI GKGCMC 3 cut(s) 1675, 2527, 3658
BstUI CGCG 4 cut(s) 2921, 3591, 3604, 3773
BstX2I RGATCY 4 cut(s) 1016, 1636, 1846, 1985
BstXI CCANNNNNNTGG 1 cut(s) 1027
BstYI RGATCY 4 cut(s) 1016, 1636, 1846, 1985
Bsu36I CCTNAGG 1 cut(s) 1545
BsuI GTATCC 2 cut(s) 43, 2360
BsuRI GGCC 8 cut(s) 5, 18, 374, 2102, 2525, 2823, 3187, 3497
BtgI CCRYGG 5 cut(s) 549, 718, 1560, 2991, 3000
BtgZI GCGATG 1 cut(s) 2191
BtrI CACGTC 1 cut(s) 2126
BtsI GCAGTG 1 cut(s) 1257
BtsIMutI CAGTG 7 cut(s) 495, 1257, 1773, 2184, 2523, 2698, 2863
BveI ACCTGC 3 cut(s) 356, 875, 2570
CaiI CAGNNNCTG 2 cut(s) 1505, 1688
CciI TCATGA 1 cut(s) 2679
CfoI GCGC 4 cut(s) 29, 2542, 3604, 3875
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 4 cut(s) 2523, 2524, 2822, 3717
CseI GACGC 1 cut(s) 2750
Csp6I GTAC 6 cut(s) 32, 870, 1427, 1874, 2360, 2803
CspCI CAANNNNNGTGG 4 cut(s) 2044, 2079, 3709, 3744
CviQI GTAC 6 cut(s) 32, 870, 1427, 1874, 2360, 2803
DraI TTTAAA 1 cut(s) 3172
DrdI GACNNNNNNGTC 2 cut(s) 1562, 1962
DriI GACNNNNNGTC 2 cut(s) 2331, 3088
DseDI GACNNNNNNGTC 2 cut(s) 1562, 1962
EaeI YGGCCR 4 cut(s) 3, 372, 3185, 3495
Eam1104I CTCTTC 4 cut(s) 69, 387, 1242, 1482
Eam1105I GACNNNNNGTC 2 cut(s) 2331, 3088
EarI CTCTTC 4 cut(s) 69, 387, 1242, 1482
EciI GGCGGA 2 cut(s) 141, 2132
Eco130I CCWWGG 8 cut(s) 327, 549, 718, 927, 1560, 2329, 2991, 3852
Eco24I GRGCYC 4 cut(s) 103, 1901, 2527, 3623
Eco31I GGTCTC 3 cut(s) 337, 394, 2140
Eco32I GATATC 1 cut(s) 2340
Eco47I GGWCC 1 cut(s) 3717
Eco47III AGCGCT 1 cut(s) 3874
Eco57I CTGAAG 3 cut(s) 42, 368, 1872
Eco81I CCTNAGG 1 cut(s) 1545
EcoO109I RGGNCCY 1 cut(s) 2524
EcoRI GAATTC 1 cut(s) 1999
EcoRII CCWGG 7 cut(s) 18, 1416, 2109, 2249, 2606, 3047, 3246
EcoRV GATATC 1 cut(s) 2340
EcoT14I CCWWGG 8 cut(s) 327, 549, 718, 927, 1560, 2329, 2991, 3852
EcoT22I ATGCAT 3 cut(s) 3694, 3850, 3886
EcoT38I GRGCYC 4 cut(s) 103, 1901, 2527, 3623
ErhI CCWWGG 8 cut(s) 327, 549, 718, 927, 1560, 2329, 2991, 3852
FaqI GGGAC 1 cut(s) 281
FauI CCCGC 3 cut(s) 2514, 2914, 3569
FbaI TGATCA 4 cut(s) 1570, 2586, 2853, 3822
FblI GTMKAC 1 cut(s) 2901
FriOI GRGCYC 4 cut(s) 103, 1901, 2527, 3623
FspBI CTAG 6 cut(s) 905, 1248, 2528, 2543, 3039, 3105
GlaI GCGC 4 cut(s) 28, 2541, 3603, 3874
GsuI CTGGAG 4 cut(s) 519, 543, 819, 900
HaeII RGCGCY 2 cut(s) 30, 3876
HaeIII GGCC 8 cut(s) 5, 18, 374, 2102, 2525, 2823, 3187, 3497
HapII CCGG 5 cut(s) 87, 2571, 3117, 3311, 3715
HgaI GACGC 1 cut(s) 2750
HhaI GCGC 4 cut(s) 29, 2542, 3604, 3875
Hin6I GCGC 4 cut(s) 27, 2540, 3602, 3873
HinP1I GCGC 4 cut(s) 27, 2540, 3602, 3873
HincII GTYRAC 4 cut(s) 1296, 1555, 1729, 2285
HindII GTYRAC 4 cut(s) 1296, 1555, 1729, 2285
HindIII AAGCTT 2 cut(s) 2194, 3166
HpaII CCGG 5 cut(s) 87, 2571, 3117, 3311, 3715
HphI GGTGA 6 cut(s) 1032, 1352, 1393, 1580, 3467, 3715
Hpy99I CGWCG 2 cut(s) 2939, 2966
HpyAV CCTTC 8 cut(s) 484, 553, 860, 1847, 2782, 2802, 2813, 2828
HpyCH4IV ACGT 4 cut(s) 468, 2125, 2404, 2964
HpySE526I ACGT 4 cut(s) 468, 2125, 2404, 2964
HspAI GCGC 4 cut(s) 27, 2540, 3602, 3873
Kpn2I TCCGGA 1 cut(s) 3310
Ksp22I TGATCA 4 cut(s) 1570, 2586, 2853, 3822
LmnI GCTCC 6 cut(s) 776, 881, 1856, 2751, 2924, 3880
MaeI CTAG 6 cut(s) 905, 1248, 2528, 2543, 3039, 3105
MaeII ACGT 4 cut(s) 468, 2125, 2404, 2964
MaeIII GTNAC 4 cut(s) 403, 821, 2075, 3107
MbiI CCGCTC 2 cut(s) 123, 2831
MboII GAAGA 5 cut(s) 86, 374, 1229, 1499, 2177
MflI RGATCY 4 cut(s) 1016, 1636, 1846, 1985
MhlI GDGCHC 7 cut(s) 103, 1675, 1766, 1901, 2527, 3623, 3658
MlsI TGGCCA 1 cut(s) 3497
MluI ACGCGT 1 cut(s) 3589
MluNI TGGCCA 1 cut(s) 3497
MlyI GAGTC 2 cut(s) 1972, 3122
MmeI TCCRAC 6 cut(s) 544, 1252, 1976, 2512, 3232, 3618
Mox20I TGGCCA 1 cut(s) 3497
Mph1103I ATGCAT 3 cut(s) 3694, 3850, 3886
MroI TCCGGA 1 cut(s) 3310
MroXI GAANNNNTTC 1 cut(s) 2687
MscI TGGCCA 1 cut(s) 3497
MseI TTAA 8 cut(s) 1083, 1446, 2396, 2556, 3171, 3218, 3414, 3780
MslI CAYNNNNRTG 2 cut(s) 899, 1042
Msp20I TGGCCA 1 cut(s) 3497
MspA1I CMGCKG 3 cut(s) 677, 1505, 2105
MspI CCGG 5 cut(s) 87, 2571, 3117, 3311, 3715
MspR9I CCNGG 7 cut(s) 20, 1418, 2111, 2251, 2608, 3049, 3248
Mva1269I GAATGC 4 cut(s) 1055, 2618, 3058, 3763
MvaI CCWGG 7 cut(s) 20, 1418, 2111, 2251, 2608, 3049, 3248
MvnI CGCG 4 cut(s) 2921, 3591, 3604, 3773
NcoI CCATGG 4 cut(s) 549, 718, 1560, 2991
NheI GCTAGC 1 cut(s) 2542
NlaIV GGNNCC 8 cut(s) 220, 772, 982, 1018, 1255, 1422, 1848, 2525
NmeAIII GCCGAG 2 cut(s) 31, 3163
NmuCI GTSAC 2 cut(s) 2075, 3107
NsiI ATGCAT 3 cut(s) 3694, 3850, 3886
NspI RCATGY 1 cut(s) 244
PaeI GCATGC 1 cut(s) 244
PagI TCATGA 1 cut(s) 2679
PaqCI CACCTGC 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 3792
PctI GAATGC 4 cut(s) 1055, 2618, 3058, 3763
PdmI GAANNNNTTC 1 cut(s) 2687
PflFI GACNNNGTC 1 cut(s) 272
PflMI CCANNNNNTGG 1 cut(s) 1337
Ple19I CGATCG 1 cut(s) 360
PleI GAGTC 2 cut(s) 1971, 3121
PpsI GAGTC 2 cut(s) 1971, 3121
PsiI TTATAA 2 cut(s) 608, 701
Psp6I CCWGG 7 cut(s) 18, 1416, 2109, 2249, 2606, 3047, 3246
PspGI CCWGG 7 cut(s) 18, 1416, 2109, 2249, 2606, 3047, 3246
PspN4I GGNNCC 8 cut(s) 220, 772, 982, 1018, 1255, 1422, 1848, 2525
PspOMI GGGCCC 1 cut(s) 2523
PspPI GGNCC 4 cut(s) 2523, 2524, 2822, 3717
PsrI GAACNNNNNNTAC 2 cut(s) 3546, 3578
PstI CTGCAG 1 cut(s) 1078
PstNI CAGNNNCTG 2 cut(s) 1505, 1688
PsuI RGATCY 4 cut(s) 1016, 1636, 1846, 1985
PsyI GACNNNGTC 1 cut(s) 272
PvuI CGATCG 1 cut(s) 360
PvuII CAGCTG 2 cut(s) 677, 1505
RsaI GTAC 6 cut(s) 33, 871, 1428, 1875, 2361, 2804
RsaNI GTAC 6 cut(s) 32, 870, 1427, 1874, 2360, 2803
RseI CAYNNNNRTG 2 cut(s) 899, 1042
SaqAI TTAA 8 cut(s) 1083, 1446, 2396, 2556, 3171, 3218, 3414, 3780
Sau96I GGNCC 4 cut(s) 2523, 2524, 2822, 3717
SchI GAGTC 2 cut(s) 1972, 3122
ScrFI CCNGG 7 cut(s) 20, 1418, 2111, 2251, 2608, 3049, 3248
SduI GDGCHC 7 cut(s) 103, 1675, 1766, 1901, 2527, 3623, 3658
SfcI CTRYAG 2 cut(s) 1074, 1614
SinI GGWCC 1 cut(s) 3717
SmiMI CAYNNNNRTG 2 cut(s) 899, 1042
SmlI CTYRAG 5 cut(s) 36, 1048, 1841, 1920, 2473
SmoI CTYRAG 5 cut(s) 36, 1048, 1841, 1920, 2473
SphI GCATGC 1 cut(s) 244
SspI AATATT 2 cut(s) 1221, 1456
SspMI CTAG 6 cut(s) 905, 1248, 2528, 2543, 3039, 3105
StyD4I CCNGG 7 cut(s) 18, 1416, 2109, 2249, 2606, 3047, 3246
StyI CCWWGG 8 cut(s) 327, 549, 718, 927, 1560, 2329, 2991, 3852
TaiI ACGT 4 cut(s) 471, 2128, 2407, 2967
TaqI TCGA 7 cut(s) 106, 656, 1961, 2937, 3275, 3739, 3746
TatI WGTACW 2 cut(s) 1426, 2802
TauI GCSGC 5 cut(s) 126, 377, 1265, 2105, 2148
Tru1I TTAA 8 cut(s) 1083, 1446, 2396, 2556, 3171, 3218, 3414, 3780
Tru9I TTAA 8 cut(s) 1083, 1446, 2396, 2556, 3171, 3218, 3414, 3780
TscAI CASTG 7 cut(s) 502, 1264, 1773, 2184, 2523, 2698, 2863
TseFI GTSAC 2 cut(s) 2075, 3107
Tsp45I GTSAC 2 cut(s) 2075, 3107
TspGWI ACGGA 4 cut(s) 107, 1978, 3416, 3801
TspRI CASTG 7 cut(s) 502, 1264, 1773, 2184, 2523, 2698, 2863
Tth111I GACNNNGTC 1 cut(s) 272
Van91I CCANNNNNTGG 1 cut(s) 1337
VneI GTGCAC 2 cut(s) 1671, 3654
VpaK11BI GGWCC 1 cut(s) 3717
XapI RAATTY 9 cut(s) 341, 569, 1373, 1526, 1644, 1999, 2954, 3353, 3509
XceI RCATGY 1 cut(s) 244
XcmI CCANNNNNNNNNTGG 1 cut(s) 1264
XmiI GTMKAC 1 cut(s) 2901
XmnI GAANNNNTTC 1 cut(s) 2687
XspI CTAG 6 cut(s) 905, 1248, 2528, 2543, 3039, 3105
Zsp2I ATGCAT 3 cut(s) 3694, 3850, 3886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.