Rroxscaffold_2G00130200

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000116
Physical Location & Seq
Reverse (-)
66298232 .. 66302238
4007 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00130200.1

Sequence Viewer

Length: 570 bp
ATGAGAGGGAGGCGGCGTCGGAGGGAGAGGGAGGCGGCGTCGGAGGGAGAGTGGAGATGGGATAGAGATGAAAATGCCGTGCGCCCTCGATCTCATCTCGAAGGCTCGATGGCAATCCGTAATCGCCTCTCCCAAGGGAGGATAGAATCTGTCATTTTGAAACATTCATTTAGCTACGGTAAGTACCGCTACGGTAAGCTGCGGCTTCGGCTTCGAAAGCCGGGTCAGCAGTGGGAGGTATGTTCCACGCGCGCCGTCCTCATGCATTTGGAGCCAGGGACCATGGACAGCATCAGATCTGGACTCTACGGTCAGATATTCTGTCCCGATAACTTCGTCGTCGGTCAGTCTTGTGCTGGAAACAACTGGGCAACAGGGGAAGACATGAGAATTGGAGAGCGGATTGGGAAAGGTAATGATCTTGATGTTTGCCCACTGCCTACAAAACAAAGTGGAACTGTATACCATGGTCTGTGGGATGGATCAGATGTTGCTGTTAAGGTATTCTTGAAGCAAGAATATCCAGATGATGTGCTTCTTTCTTTTAGACAAGAACACTTCTTGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

189

Amino Acids

21.74

Weight (kDa)

9.44

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02520 AT1G02520 AT1G02520 AT1G02530 AT1G02530 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT3G62150 AT3G62150 AT3G62150 AT4G01820 AT4G01820 AT4G01830 AT4G01830
fragaria_vesca FvH4_1g26280 FvH4_4g21370 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21381 FvH4_4g21600 FvH4_4g21600 FvH4_4g21600 FvH4_4g21620 FvH4_4g21621 FvH4_4g21622 FvH4_6g00641 FvH4_6g00650
malus_domestica MD09G1290100.v1.1 MD13G1138600.v1.1 MD13G1138700.v1.1 MD13G1139100.v1.1 MD16G1134500.v1.1 MD16G1134700.v1.1 MD16G1135000.v1.1 MD16G1135100.v1.1 MD17G1284300.v1.1 MD17G1284700.v1.1
prunus_persica Prupe.1G103800_v2.0.a1 Prupe.1G103800_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1
pyrus_communis pycom04g08750 pycom09g19770 pycom09g19790 pycom13g09140 pycom16g11900 pycom16g11920 pycom16g11930 pycom17g28030
rosa_chinensis RchiOBHm_Chr2g0157341 RchiOBHm_Chr3g0448061 RchiOBHm_Chr3g0485931 RchiOBHm_Chr3g0485961 RchiOBHm_Chr4g0427151 RchiOBHm_Chr4g0427161 RchiOBHm_Chr4g0427211 RchiOBHm_Chr4g0427241 RchiOBHm_Chr4g0427271 RchiOBHm_Chr4g0427711 RchiOBHm_Chr4g0427721 RchiOBHm_Chr4g0427771 RchiOBHm_Chr4g0427781 RchiOBHm_Chr4g0427791 RchiOBHm_Chr4g0427851 RchiOBHm_Chr4g0427861 RchiOBHm_Chr4g0446561 RchiOBHm_Chr5g0081601 RchiOBHm_Chr6g0278251 RchiOBHm_Chr6g0303241
rosa_laevigata RLG00000007206 RLG00000007207 RLG00000007209 RLG00000007210 RLG00000007211 RLG00000007212 RLG00000007214 RLG00000007255 RLG00000007256 RLG00000007257 RLG00000011097 RLG00000019257 RLG00000023121 RLG00000025948 RLG00000027246 RLG00000029989
rosa_multiflora Rmu_co8076196.1_g000001 Rmu_co8517461.1_g000001 Rmu_sc0000066.1_g000018 Rmu_sc0000066.1_g000024 Rmu_sc0000066.1_g000033 Rmu_sc0000066.1_g000047 Rmu_sc0000066.1_g000048 Rmu_sc0001651.1_g000024 Rmu_sc0002816.1_g000003 Rmu_sc0003337.1_g000015 Rmu_sc0003444.1_g000015 Rmu_sc0003813.1_g000004 Rmu_sc0004876.1_g000001 Rmu_sc0005969.1_g000025 Rmu_sc0008650.1_g000006 Rmu_sc0008855.1_g000001 Rmu_sc0009165.1_g000001 Rmu_sc0009165.1_g000002 Rmu_sc0010010.1_g000001 Rmu_sc0010010.1_g000005 Rmu_sc0036078.1_g000001
rosa_roxburghii Rroxscaffold_2G00130200 Rroxscaffold_5G00367590 Rroxscaffold_5G00368970 Rroxscaffold_5G00368990 Rroxscaffold_5G00369340 Rroxscaffold_5G00369350 Rroxscaffold_5G00369380 Rroxscaffold_5G00369390 Rroxscaffold_5G00369440 Rroxscaffold_5G00369450 Rroxscaffold_5G00369500 Rroxscaffold_5G00369510 Rroxscaffold_6G00396190 Rroxscaffold_6G00426220 Rroxscaffold_6G00427790 Rroxscaffold_7G00164940
rosa_rugosa Rorug01G0160800.1 Rorug02G0341400 Rorug02G0461800 Rorug02G0608000 Rorug03G0222700 Rorug03G0222800 Rorug03G0222900 Rorug04G0216400 Rorug04G0216400 Rorug04G0220100 Rorug04G0220100 Rorug04G0220100 Rorug05G0181800 Rorug05G0409700
rosa_samantha Rh1BG177300 Rh1DG206800 Rh2AG590600 Rh2BG473400 Rh2DG482300 Rh3AG007500 Rh3AG272400 Rh3BG007200 Rh3BG307000 Rh3DG301600 Rh3DG301900 Rh4AG272300 Rh4AG272400 Rh4AG272700 Rh4AG275900 Rh4AG276100 Rh4AG276200 Rh4AG276500 Rh4AG276600 Rh4AG276700 Rh4AG277000 Rh4BG281900 Rh4BG282200 Rh4CG292900 Rh4CG293300 Rh4CG293400 Rh4CG293600 Rh4CG297400 Rh4CG297500 Rh4CG297900 Rh4CG298000 Rh4CG298300 Rh4CG298400 Rh4DG165900 Rh5BG011400 Rh5DG092900 Rh6AG434000 Rh6CG153300 Rh6CG391700 Rh7BG333600
rosa_wichuraiana Rw0G005570 Rw0G005590 Rw0G011850 Rw0G015530 Rw0G017350 Rw0G019580 Rw3G000600 Rw3G024160 Rw4G023620 Rw4G023640 Rw4G023660 Rw4G023670 Rw4G023950 Rw4G023960 Rw4G024050 Rw4G024060 Rw5G048020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 309
AccBSI CCGCTC 1 cut(s) 400
AccI GTMKAC 1 cut(s) 462
AccII CGCG 2 cut(s) 250, 252
AciI CCGC 5 cut(s) 13, 35, 187, 202, 400
AclWI GGATC 1 cut(s) 490
AcyI GRCGYC 2 cut(s) 16, 38
AfaI GTAC 1 cut(s) 185
AfiI CCNNNNNNNGG 1 cut(s) 138
AgsI TTSAA 2 cut(s) 160, 511
AjnI CCWGG 1 cut(s) 274
AluBI AGCT 2 cut(s) 174, 199
AluI AGCT 2 cut(s) 174, 199
AlwI GGATC 1 cut(s) 490
ApeKI GCWGC 1 cut(s) 199
ArsI GACNNNNNNTTYG 2 cut(s) 208, 240
AspLEI GCGC 3 cut(s) 84, 252, 254
AspS9I GGNCC 1 cut(s) 279
AsuC2I CCSGG 1 cut(s) 222
AsuII TTCGAA 1 cut(s) 214
AvaII GGWCC 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 387
BbvI GCAGC 1 cut(s) 186
BccI CCATC 3 cut(s) 51, 103, 473
BceAI ACGGC 2 cut(s) 62, 239
BciT130I CCWGG 1 cut(s) 276
BcnI CCSGG 1 cut(s) 222
BglII AGATCT 1 cut(s) 296
BisI GCNGC 4 cut(s) 14, 36, 200, 203
BlsI GCNGC 4 cut(s) 15, 37, 201, 204
Bme1390I CCNGG 2 cut(s) 222, 276
Bme18I GGWCC 1 cut(s) 279
BmgT120I GGNCC 1 cut(s) 279
BmiI GGNNCC 2 cut(s) 273, 280
BmrFI CCNGG 2 cut(s) 222, 276
BmrI ACTGGG 1 cut(s) 376
BmsI GCATC 1 cut(s) 300
BmuI ACTGGG 1 cut(s) 376
BpiI GAAGAC 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 214
BpuMI CCSGG 1 cut(s) 222
BsaBI GATNNNNATC 1 cut(s) 113
BsaHI GRCGYC 2 cut(s) 16, 38
BsaJI CCNNGG 4 cut(s) 133, 275, 282, 466
BsaXI ACNNNNNCTCC 5 cut(s) 23, 31, 35, 53, 65
Bsc4I CCNNNNNNNGG 1 cut(s) 138
Bse1I ACTGG 1 cut(s) 371
Bse8I GATNNNNATC 1 cut(s) 113
BseBI CCWGG 1 cut(s) 276
BseDI CCNNGG 4 cut(s) 133, 275, 282, 466
BseGI GGATG 1 cut(s) 484
BseJI GATNNNNATC 1 cut(s) 113
BseLI CCNNNNNNNGG 1 cut(s) 138
BseNI ACTGG 1 cut(s) 371
BsePI GCGCGC 1 cut(s) 250
BseXI GCAGC 1 cut(s) 186
Bsh1236I CGCG 2 cut(s) 250, 252
BsiSI CCGG 1 cut(s) 221
BslFI GGGAC 2 cut(s) 292, 309
BslI CCNNNNNNNGG 1 cut(s) 138
BsmFI GGGAC 2 cut(s) 292, 309
Bsp119I TTCGAA 1 cut(s) 214
Bsp143I GATC 4 cut(s) 89, 296, 418, 482
Bsp19I CCATGG 2 cut(s) 282, 466
BspACI CCGC 5 cut(s) 13, 35, 187, 202, 400
BspFNI CGCG 2 cut(s) 250, 252
BspLI GGNNCC 2 cut(s) 273, 280
BspPI GGATC 1 cut(s) 490
BspT104I TTCGAA 1 cut(s) 214
BsrBI CCGCTC 1 cut(s) 400
BsrI ACTGG 1 cut(s) 371
BssECI CCNNGG 4 cut(s) 133, 275, 282, 466
BssHII GCGCGC 1 cut(s) 250
BssMI GATC 4 cut(s) 89, 296, 418, 482
BssNAI GTATAC 1 cut(s) 463
BssNI GRCGYC 2 cut(s) 16, 38
BssT1I CCWWGG 3 cut(s) 133, 282, 466
Bst1107I GTATAC 1 cut(s) 463
Bst2UI CCWGG 1 cut(s) 276
Bst4CI ACNGT 4 cut(s) 179, 194, 311, 460
BstACI GRCGYC 2 cut(s) 16, 38
BstBI TTCGAA 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 252
BstDSI CCRYGG 2 cut(s) 282, 466
BstF5I GGATG 1 cut(s) 484
BstFNI CGCG 2 cut(s) 250, 252
BstHHI GCGC 3 cut(s) 84, 252, 254
BstKTI GATC 4 cut(s) 92, 299, 421, 485
BstMBI GATC 4 cut(s) 89, 296, 418, 482
BstMWI GCNNNNNNNGC 4 cut(s) 208, 217, 226, 271
BstNI CCWGG 1 cut(s) 276
BstSCI CCNGG 2 cut(s) 220, 274
BstUI CGCG 2 cut(s) 250, 252
BstV1I GCAGC 1 cut(s) 186
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 1 cut(s) 296
BstYI RGATCY 1 cut(s) 296
BstZ17I GTATAC 1 cut(s) 463
BtgI CCRYGG 2 cut(s) 282, 466
BtsCI GGATG 1 cut(s) 484
BtsI GCAGTG 2 cut(s) 236, 434
BtsIMutI CAGTG 2 cut(s) 236, 434
Cac8I GCNNGC 1 cut(s) 252
CfoI GCGC 3 cut(s) 84, 252, 254
Cfr13I GGNCC 1 cut(s) 279
CseI GACGC 2 cut(s) 5, 27
Csp6I GTAC 1 cut(s) 184
CviAII CATG 4 cut(s) 262, 283, 385, 467
CviJI RGCY 8 cut(s) 105, 174, 199, 205, 211, 220, 274, 567
CviKI_1 RGCY 8 cut(s) 105, 174, 199, 205, 211, 220, 274, 567
CviQI GTAC 1 cut(s) 184
DpnI GATC 4 cut(s) 91, 298, 420, 484
DpnII GATC 4 cut(s) 89, 296, 418, 482
DrdI GACNNNNNNGTC 1 cut(s) 309
DseDI GACNNNNNNGTC 1 cut(s) 309
Eco130I CCWWGG 3 cut(s) 133, 282, 466
Eco47I GGWCC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 274
EcoT14I CCWWGG 3 cut(s) 133, 282, 466
EcoT22I ATGCAT 1 cut(s) 267
ErhI CCWWGG 3 cut(s) 133, 282, 466
FaeI CATG 4 cut(s) 265, 286, 388, 470
FaiI YATR 6 cut(s) 241, 263, 284, 386, 463, 468
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FaqI GGGAC 2 cut(s) 292, 309
FatI CATG 4 cut(s) 261, 282, 384, 466
FblI GTMKAC 1 cut(s) 462
Fnu4HI GCNGC 4 cut(s) 14, 36, 200, 203
FokI GGATG 1 cut(s) 491
Fsp4HI GCNGC 4 cut(s) 14, 36, 200, 203
GlaI GCGC 3 cut(s) 83, 251, 253
GluI GCNGC 4 cut(s) 14, 36, 200, 203
HapII CCGG 1 cut(s) 221
HgaI GACGC 2 cut(s) 5, 27
HhaI GCGC 3 cut(s) 84, 252, 254
Hin1I GRCGYC 2 cut(s) 16, 38
Hin1II CATG 4 cut(s) 265, 286, 388, 470
Hin6I GCGC 3 cut(s) 82, 250, 252
HinP1I GCGC 3 cut(s) 82, 250, 252
HinfI GANTC 2 cut(s) 146, 303
HpaII CCGG 1 cut(s) 221
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 5 cut(s) 21, 43, 296, 315, 487
Hpy188III TCNNGA 6 cut(s) 98, 300, 326, 422, 508, 524
Hpy8I GTNNAC 1 cut(s) 463
Hpy99I CGWCG 4 cut(s) 21, 43, 341, 344
HpyAV CCTTC 1 cut(s) 95
HpyCH4III ACNGT 4 cut(s) 179, 194, 311, 460
HpyCH4V TGCA 1 cut(s) 265
HpyF10VI GCNNNNNNNGC 4 cut(s) 208, 217, 226, 271
Hsp92I GRCGYC 2 cut(s) 16, 38
Hsp92II CATG 4 cut(s) 265, 286, 388, 470
HspAI GCGC 3 cut(s) 82, 250, 252
Kzo9I GATC 4 cut(s) 89, 296, 418, 482
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 8 cut(s) 234, 261, 285, 288, 342, 352, 360, 537
Lsp1109I GCAGC 1 cut(s) 186
LweI GCATC 1 cut(s) 300
MalI GATC 4 cut(s) 91, 298, 420, 484
MbiI CCGCTC 1 cut(s) 400
MboI GATC 4 cut(s) 89, 296, 418, 482
MboII GAAGA 1 cut(s) 392
MflI RGATCY 1 cut(s) 296
MluCI AATT 1 cut(s) 390
MlyI GAGTC 1 cut(s) 297
MmeI TCCRAC 1 cut(s) 21
Mph1103I ATGCAT 1 cut(s) 267
MseI TTAA 1 cut(s) 498
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 2 cut(s) 222, 276
MvaI CCWGG 1 cut(s) 276
MvnI CGCG 2 cut(s) 250, 252
MwoI GCNNNNNNNGC 4 cut(s) 208, 217, 226, 271
NciI CCSGG 1 cut(s) 222
NcoI CCATGG 2 cut(s) 282, 466
NdeII GATC 4 cut(s) 89, 296, 418, 482
NlaIII CATG 4 cut(s) 265, 286, 388, 470
NlaIV GGNNCC 2 cut(s) 273, 280
NsiI ATGCAT 1 cut(s) 267
NspV TTCGAA 1 cut(s) 214
PauI GCGCGC 1 cut(s) 250
PfeI GAWTC 1 cut(s) 146
PkrI GCNGC 4 cut(s) 15, 37, 201, 204
PleI GAGTC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 274
PspGI CCWGG 1 cut(s) 274
PspN4I GGNNCC 2 cut(s) 273, 280
PspPI GGNCC 1 cut(s) 279
PsuI RGATCY 1 cut(s) 296
PteI GCGCGC 1 cut(s) 250
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
SaqAI TTAA 1 cut(s) 498
SatI GCNGC 4 cut(s) 14, 36, 200, 203
Sau3AI GATC 4 cut(s) 89, 296, 418, 482
Sau96I GGNCC 1 cut(s) 279
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 2 cut(s) 222, 276
SetI ASST 5 cut(s) 176, 201, 240, 415, 504
SfaNI GCATC 1 cut(s) 300
SfuI TTCGAA 1 cut(s) 214
SinI GGWCC 1 cut(s) 279
Sse9I AATT 1 cut(s) 390
SsiI CCGC 5 cut(s) 13, 35, 187, 202, 400
StyD4I CCNGG 2 cut(s) 220, 274
StyI CCWWGG 3 cut(s) 133, 282, 466
TaaI ACNGT 4 cut(s) 179, 194, 311, 460
TaqI TCGA 4 cut(s) 88, 99, 107, 214
TaqII GACCGA 1 cut(s) 332
TasI AATT 1 cut(s) 390
TauI GCSGC 3 cut(s) 16, 38, 205
TfiI GAWTC 1 cut(s) 146
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TscAI CASTG 2 cut(s) 236, 441
TseI GCWGC 1 cut(s) 199
TspDTI ATGAA 2 cut(s) 84, 156
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 2 cut(s) 236, 441
VpaK11BI GGWCC 1 cut(s) 279
XmiI GTMKAC 1 cut(s) 462
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.