Rroxscaffold_5G00369510

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000116
Physical Location & Seq
Forward (+)
51323386 .. 51336003
12618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369510.1

Sequence Viewer

Length: 3678 bp
ATGGCTGAGGAGAATCCTTCAGATGGAGATGCAATAACCAAGCAAGAAACTCCAACAAATAGCCATCCAACAGTGATGGTGGGTTCCAACGAAAACAGACTAGATACAAGCAAGAGCAAGGAGGATGGAACTAAAACAGTTCCACTCTACAAACTTTTCTCTTTTGCTGATTCCATGGATTACCTGTTAATGTCTGTTGGTACAATCAGCGCTATTGGTAATGGACTCTGTGGCTCTAAAGTATGTTTACTTGGCTCTGGGGGTGCTGCTGCAGCATTTCTACAGATGTCATGCTGGATGGTCACTGGAGAGAGACAGGCTGCACGATTACGAAGTTTATACTTGAAGACCATACTGAGGCAAGATGTCAGCTATTTTGATCAAGAAATCAACTCTGGAGAAATTGTTGGGAGGATGTCAGGTGATACTCTGCTCATTAAAGAGGCGATGGGTGAGAAGGTAGGAACTTTTATCCAAGTAGTTGCAACATTCACAGGCAGCTATATTATAGCATTTTTTAAGGGATGGCTTCTCACCCTTGTCATGATATCCTCTATTCCCCCTCTTGTCTTCTCTGGTGCTGTCATGAACCATTTCATAGTGAAATTGGCATGCAATGGACAAGCTGCATATTCAGGAGGAGCAACTGTGGTAGAGGAGACAATTGGTTCTATCAGAACTGTTGCATCGTTCACAGGGGAGAAGCAAGCTATATCTAACTACAATAATTCCTTGAATAAAGCTTACAAGTCCGGTGTGCAAGAGGGTTGGACATCTGGTTTGGGTTCTGGTGCACTTAGGCTTATGGTATTCTGTACTTATGCTTTGGCTGTGTGGTTTGGTGGAATAATGATACTTGAAAAAGGATACAAGGGAGGAGATGTCATGACAGTAATTTTTTCTGTGTTGATGGGCTCTCTGTCTCTTGGGAAAGCATCTCCTTGCATCAGTGCATTTGCTCCTGGACAAGCTGCAGCTTATAAGATGTTTGAGACAATTAACAGAAAGCCCGAGATAGATGCATATGATACTACTGGGAGGGTATTACAAGATATTCGTGGAGACATAGAACTGAGGGATGTTTGTTTTAGTTATCCCACAAGACTGGATGAGCAAATATTCCATGGATTTTGTGTCTCAATACCTAGTGGTGCAACTGCTGCTTTGGTTGGAGAAAGCGGAAGTGGTAAATCTACTGTACTTGGTTTGATTGAGAGATTTTATGACCCCCAGGCTGGTGAAGTTCTTATTGACGGTGTTAATATCAAAGAGTTCCAACTGAAATGGATCAGACAGAAAATAGGCCTTGTAAGCCAGGAACCAGTTTTGTTTACTGGTAGCATTAAAGATAATATTGCGTTTGGAAAGGATGGTGCTACTACTGAAGAGATAGCAACTGCTGCTGAGCTTGGCAAAGCTGCAAAATTCATAGCTTTACTGCCTCAGGGAATAGATACCTTGGTTGGTGAGCATGGAACTCAGCTATCTGGGGGGCAAAAGCAAAGAGTTGCTATAGCCAGAGCAATTCTGAAGAACCCGAGAATTCTACTTTTAGATGAAGCGACAAGCGCACTTGACACAAAATCCGAGAGAATTGTGCAGGAGGCGCTGGACAGAGTAATGATTAACCGGACTACTGTCATAGTAGCCCACCGCTTGAGCACAGTAAGGAATGCTGACACCATTGCTGTAATACATCAGGGAATAATTGCTGAGGAAGGACCACATTCTGAGCTAATCAGGGATCCCGATGGAGCATATAGCAGGCTTATAAGGTTGCAAGAAACTAGTTATCTGTCAGAAGACTCGGAACATACTTGTCTAAATTCTGAGAAATCATCTCCAAGGAGAAGCAGTAGTCATCACTCATTCTCAATCTCATATGGTATTCCTAATGCAAGCGGTATCCTTGAAACAACAGCTATAGAACCTGACGCTCCTGCTTCAGTACCAACAAAAGGGCCTCTAGGAGTCTCTCTTCACCGCGTTGCTTACCTGAACGAGCCAGAGATACCAGTGCTACTTCTAGGTACTATAGTTGCAGCTGTCAATGGGGTAATCTTCCCTGTTTATGGTAGTTTGTTGTCAAGTGTAATCAAGACCTTCTTTGAGCCACCCCATGAACTTCGTAAGCATTCAAAGTTTTTGGCATCAATCTTTGTTATTCTTGGAGTGGTATCTTTCTTGGCACAATCGTCAAGAGCACACCTCTTTGCCGTGGCGAGCTGCAGGTTAATAAGAAGAGTCCGCGCAATGTGCTTTGAGAAGGTAGTTTACATGGAAGTAAGTTGGTTTGATGAAGCTGAGCACTCAAGTGGTGCCATTGGGGCAAGGCTTTCTACAGATGCAGCGTCTTTGAGAGGGCTTGTTGGAGATGCTCTTGGTTTGGTGGTTGAGAATACTGCAGCAGCAATTATGGGGGTGATTATTGCTTTTGGTGCAAATTGGCAACTTTCTCTTGTAATCCTTGTTCTGTTACCTTTATTGGGAATAAACGGATATCTTCAAGACCAGTACATGAAAGGATTCAGTGCAGATGCAAAGAAATTGTACGAGGACGCAAGCCAAGTAGCTAGTGATGCTGTGGGGAGTATTCGGACAGTTGCTTCCTTTTGTGCAGAAGAGAAGATGATTGAGTTGTACAAGAAAAAATCGTTTGCCTTAATATTTTACACTGGTGGTCTACTTGTTGCAGCAGGCAAGACAACAGCCTCTGCTGTTTTCCGGGCAAGTTGCTCTGCGTTGTGCTATAAAACTATTTTAGCAGCTGTCTTTGCTCTCACTATTGCTTCTTTTGGACTTGCTCAGTCGAGCTCCCTAGCAACTGATGTTAGTAGAGGAAAGAGCTCTGCTGCTTCCATATTCGAAATTCTTGACATGAAATCAAAAATAGACTCTAGTGATAACTCGGGCATGACAATAGAAAATGTGAAGGGAGAACTTGAATTTCACCACGTCAATTTCAAGTATCCTAGTAGACCTAACGTGCCAATTTTCAACGATCTTTGCTTGACCATTAGTTGTGGCGAGACAATTGCTCTGGTTGGTGAAAGTGGAAGTGGAAAATCGACAGTGATCTCTTTGATCCAGAGATTTTACGATCCTGATTCAGGTCACATTACATTGGATGGAATCGTAATTCAGAAACTAGAGTTGAGGTGGTTGAGACAGCAAATGGGGCTGGTGAGCCAGGAGCCTCTGCTGTTTAATGACACTATCCGAGCCAACATTGCATATGGAAAGGAAGGAGAGACAACCGAGGCTGAAATTATAGCTGCAGCAGAACTGGCAAATGCACACGAGTTCATTAGTAGTTTACAACATGGGTATGATACAATAGTAGGAGAGCGAGGGGTCCAATTATCCGGTGGACAGAAGCAAAGAGTTGCTATTGCCCGAGCTATTATGAAGGCACCAAAGATATTACTACTTGATGAAGCCACCAGTGCTCTTGATGCTGAATCCGAACGAGTGGTGCAAGATGCCTTGGACCGAGTTATTGTGGATCGAACCACAGTGGTTGTTGCCCATCGGTTATCCACAATAAAAGGTGCAGATTTAATTGCTGTGGTAAAAAATGGAGTCGTTGCAGAGAAAGGAAAGCATGAAACTTTGATCAATATCCGCAATGGCATTTATGCATCTTTGGTAGCATTGCCTGCAAGTGCCTCAACTTAA

Protein Analysis

1225

Amino Acids

131.49

Weight (kDa)

6.07

Isoelectric Point (pI)

34.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 83 - 307 1e-51 ABC transporter transmembrane region
ABC_tran PF00005 379 - 523 3.3e-32 ABC transporter
ABC_membrane PF00664 674 - 884 6e-41 ABC transporter transmembrane region
ABC_tran PF00005 1000 - 1149 3.4e-34 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02520 AT1G02520 AT1G02520 AT1G02530 AT1G02530 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT3G62150 AT3G62150 AT3G62150 AT4G01820 AT4G01820 AT4G01830 AT4G01830
fragaria_vesca FvH4_1g26280 FvH4_4g21370 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21381 FvH4_4g21600 FvH4_4g21600 FvH4_4g21600 FvH4_4g21620 FvH4_4g21621 FvH4_4g21622 FvH4_6g00641 FvH4_6g00650
malus_domestica MD09G1290100.v1.1 MD13G1138600.v1.1 MD13G1138700.v1.1 MD13G1139100.v1.1 MD16G1134500.v1.1 MD16G1134700.v1.1 MD16G1135000.v1.1 MD16G1135100.v1.1 MD17G1284300.v1.1 MD17G1284700.v1.1
prunus_persica Prupe.1G103800_v2.0.a1 Prupe.1G103800_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1
pyrus_communis pycom04g08750 pycom09g19770 pycom09g19790 pycom13g09140 pycom16g11900 pycom16g11920 pycom16g11930 pycom17g28030
rosa_chinensis RchiOBHm_Chr2g0157341 RchiOBHm_Chr3g0448061 RchiOBHm_Chr3g0485931 RchiOBHm_Chr3g0485961 RchiOBHm_Chr4g0427151 RchiOBHm_Chr4g0427161 RchiOBHm_Chr4g0427211 RchiOBHm_Chr4g0427241 RchiOBHm_Chr4g0427271 RchiOBHm_Chr4g0427711 RchiOBHm_Chr4g0427721 RchiOBHm_Chr4g0427771 RchiOBHm_Chr4g0427781 RchiOBHm_Chr4g0427791 RchiOBHm_Chr4g0427851 RchiOBHm_Chr4g0427861 RchiOBHm_Chr4g0446561 RchiOBHm_Chr5g0081601 RchiOBHm_Chr6g0278251 RchiOBHm_Chr6g0303241
rosa_laevigata RLG00000007206 RLG00000007207 RLG00000007209 RLG00000007210 RLG00000007211 RLG00000007212 RLG00000007214 RLG00000007255 RLG00000007256 RLG00000007257 RLG00000011097 RLG00000019257 RLG00000023121 RLG00000025948 RLG00000027246 RLG00000029989
rosa_multiflora Rmu_co8076196.1_g000001 Rmu_co8517461.1_g000001 Rmu_sc0000066.1_g000018 Rmu_sc0000066.1_g000024 Rmu_sc0000066.1_g000033 Rmu_sc0000066.1_g000047 Rmu_sc0000066.1_g000048 Rmu_sc0001651.1_g000024 Rmu_sc0002816.1_g000003 Rmu_sc0003337.1_g000015 Rmu_sc0003444.1_g000015 Rmu_sc0003813.1_g000004 Rmu_sc0004876.1_g000001 Rmu_sc0005969.1_g000025 Rmu_sc0008650.1_g000006 Rmu_sc0008855.1_g000001 Rmu_sc0009165.1_g000001 Rmu_sc0009165.1_g000002 Rmu_sc0010010.1_g000001 Rmu_sc0010010.1_g000005 Rmu_sc0036078.1_g000001
rosa_roxburghii Rroxscaffold_2G00130200 Rroxscaffold_5G00367590 Rroxscaffold_5G00368970 Rroxscaffold_5G00368990 Rroxscaffold_5G00369340 Rroxscaffold_5G00369350 Rroxscaffold_5G00369380 Rroxscaffold_5G00369390 Rroxscaffold_5G00369440 Rroxscaffold_5G00369450 Rroxscaffold_5G00369500 Rroxscaffold_5G00369510 Rroxscaffold_6G00396190 Rroxscaffold_6G00426220 Rroxscaffold_6G00427790 Rroxscaffold_7G00164940
rosa_rugosa Rorug01G0160800.1 Rorug02G0341400 Rorug02G0461800 Rorug02G0608000 Rorug03G0222700 Rorug03G0222800 Rorug03G0222900 Rorug04G0216400 Rorug04G0216400 Rorug04G0220100 Rorug04G0220100 Rorug04G0220100 Rorug05G0181800 Rorug05G0409700
rosa_samantha Rh1BG177300 Rh1DG206800 Rh2AG590600 Rh2BG473400 Rh2DG482300 Rh3AG007500 Rh3AG272400 Rh3BG007200 Rh3BG307000 Rh3DG301600 Rh3DG301900 Rh4AG272300 Rh4AG272400 Rh4AG272700 Rh4AG275900 Rh4AG276100 Rh4AG276200 Rh4AG276500 Rh4AG276600 Rh4AG276700 Rh4AG277000 Rh4BG281900 Rh4BG282200 Rh4CG292900 Rh4CG293300 Rh4CG293400 Rh4CG293600 Rh4CG297400 Rh4CG297500 Rh4CG297900 Rh4CG298000 Rh4CG298300 Rh4CG298400 Rh4DG165900 Rh5BG011400 Rh5DG092900 Rh6AG434000 Rh6CG153300 Rh6CG391700 Rh7BG333600
rosa_wichuraiana Rw0G005570 Rw0G005590 Rw0G011850 Rw0G015530 Rw0G017350 Rw0G019580 Rw3G000600 Rw3G024160 Rw4G023620 Rw4G023640 Rw4G023660 Rw4G023670 Rw4G023950 Rw4G023960 Rw4G024050 Rw4G024060 Rw5G048020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 981, 1772
Acc36I ACCTGC 1 cut(s) 2220
AccB1I GGYRCC 2 cut(s) 2318, 3412
AccI GTMKAC 2 cut(s) 2683, 2977
AccII CGCG 2 cut(s) 1986, 2250
AciI CCGC 6 cut(s) 1179, 1654, 1902, 1984, 2248, 3625
AclWI GGATC 6 cut(s) 1295, 1739, 1752, 3079, 3095, 3513
AcsI RAATTY 5 cut(s) 1424, 1542, 1825, 2868, 2945
AcuI CTGAAG 3 cut(s) 1404, 1550, 1929
AfaI GTAC 8 cut(s) 202, 817, 1200, 1950, 2032, 2516, 2552, 2642
AfeI AGCGCT 1 cut(s) 211
AfiI CCNNNNNNNGG 7 cut(s) 23, 357, 1235, 1958, 2072, 2486, 3110
AgsI TTSAA 9 cut(s) 346, 736, 860, 1913, 2139, 2507, 2945, 2965, 2998
AhlI ACTAGT 1 cut(s) 1787
AjiI CACGTC 1 cut(s) 2956
AjnI CCWGG 4 cut(s) 961, 1230, 1314, 3189
AleI CACNNNNGTG 1 cut(s) 2313
Alw21I GWGCWC 7 cut(s) 796, 1664, 2206, 2310, 2816, 2849, 3451
Alw44I GTGCAC 1 cut(s) 792
AlwI GGATC 6 cut(s) 1295, 1739, 1752, 3079, 3095, 3513
AlwNI CAGNNNCTG 1 cut(s) 2714
Ama87I CYCGRG 4 cut(s) 1010, 1537, 2908, 3396
Aor51HI AGCGCT 1 cut(s) 211
AoxI GGCC 2 cut(s) 1303, 1961
ApaLI GTGCAC 1 cut(s) 792
ApoI RAATTY 5 cut(s) 1424, 1542, 1825, 2868, 2945
ArsI GACNNNNNNTTYG 2 cut(s) 763, 795
Asp700I GAANNNNTTC 2 cut(s) 593, 2525
AspLEI GCGC 4 cut(s) 212, 1571, 1609, 2252
AspS9I GGNCC 4 cut(s) 1721, 1961, 3355, 3490
AsuC2I CCSGG 1 cut(s) 2726
AsuII TTCGAA 1 cut(s) 2865
AvaI CYCGRG 4 cut(s) 1010, 1537, 2908, 3396
AvaII GGWCC 3 cut(s) 1721, 3355, 3490
AxyI CCTNAGG 1 cut(s) 1443
BaeGI GKGCMC 1 cut(s) 796
BaeI ACNNNNGTAYC 6 cut(s) 1446, 1479, 2022, 2055, 3324, 3357
BamHI GGATCC 1 cut(s) 1744
BanI GGYRCC 2 cut(s) 2318, 3412
BanII GRGCYC 3 cut(s) 917, 2816, 2849
BarI GAAGNNNNNNTAC 2 cut(s) 2258, 2290
BauI CACGAG 1 cut(s) 3299
BbsI GAAGAC 3 cut(s) 353, 562, 1809
Bbv12I GWGCWC 7 cut(s) 796, 1664, 2206, 2310, 2816, 2849, 3451
BbvCI CCTCAGC 2 cut(s) 6, 1713
BceAI ACGGC 1 cut(s) 2201
BcgI CGANNNNNNTGC 4 cut(s) 1001, 1035, 3017, 3051
BciT130I CCWGG 4 cut(s) 963, 1232, 1316, 3191
BciVI GTATCC 3 cut(s) 860, 1916, 2979
BclI TGATCA 2 cut(s) 379, 3615
BcnI CCSGG 1 cut(s) 2726
BcuI ACTAGT 1 cut(s) 1787
BfoI RGCGCY 2 cut(s) 213, 1610
BfuAI ACCTGC 1 cut(s) 2220
BfuI GTATCC 3 cut(s) 860, 1916, 2979
BglI GCCNNNNNGGC 1 cut(s) 2327
BlpI GCTNAGC 2 cut(s) 1404, 2304
Bme1390I CCNGG 5 cut(s) 963, 1232, 1316, 2726, 3191
Bme18I GGWCC 3 cut(s) 1721, 3355, 3490
BmeT110I CYCGRG 4 cut(s) 1010, 1537, 2908, 3396
BmgBI CACGTC 1 cut(s) 2956
BmgT120I GGNCC 4 cut(s) 1721, 1961, 3355, 3490
BmiI GGNNCC 7 cut(s) 85, 1320, 1746, 2320, 3195, 3356, 3414
BmrFI CCNGG 5 cut(s) 963, 1232, 1316, 2726, 3191
BmrI ACTGGG 1 cut(s) 1044
BmuI ACTGGG 1 cut(s) 1044
BoxI GACNNNNGTC 1 cut(s) 1637
BpiI GAAGAC 3 cut(s) 353, 562, 1809
BplI GAGNNNNNCTC 2 cut(s) 2193, 2225
BpmI CTGGAG 2 cut(s) 327, 417
Bpu10I CCTNAGC 2 cut(s) 6, 1713
Bpu1102I GCTNAGC 2 cut(s) 1404, 2304
Bpu14I TTCGAA 1 cut(s) 2865
BpuEI CTTGAG 2 cut(s) 1678, 2296
BpuMI CCSGG 1 cut(s) 2726
BsaBI GATNNNNATC 1 cut(s) 3620
BsaJI CCNNGG 8 cut(s) 174, 1123, 1230, 1458, 1844, 2217, 3258, 3486
BsaWI WCCGGW 3 cut(s) 752, 1629, 3365
BsaXI ACNNNNNCTCC 8 cut(s) 390, 420, 633, 663, 867, 897, 1840, 1870
Bsc4I CCNNNNNNNGG 7 cut(s) 23, 357, 1235, 1958, 2072, 2486, 3110
Bse21I CCTNAGG 1 cut(s) 1443
Bse3DI GCAATG 6 cut(s) 622, 1683, 2259, 3228, 3634, 3653
Bse8I GATNNNNATC 1 cut(s) 3620
BseBI CCWGG 4 cut(s) 963, 1232, 1316, 3191
BseDI CCNNGG 8 cut(s) 174, 1123, 1230, 1458, 1844, 2217, 3258, 3486
BseGI GGATG 9 cut(s) 64, 130, 303, 420, 530, 1084, 1114, 1375, 3133
BseJI GATNNNNATC 1 cut(s) 3620
BseLI CCNNNNNNNGG 7 cut(s) 23, 357, 1235, 1958, 2072, 2486, 3110
BseMI GCAATG 6 cut(s) 622, 1683, 2259, 3228, 3634, 3653
BseRI GAGGAG 4 cut(s) 23, 654, 671, 891
BseSI GKGCMC 1 cut(s) 796
BsgI GTGCAG 5 cut(s) 306, 1619, 2553, 2637, 3573
Bsh1236I CGCG 2 cut(s) 1986, 2250
BshFI GGCC 2 cut(s) 1305, 1963
BshNI GGYRCC 2 cut(s) 2318, 3412
BsiHKAI GWGCWC 7 cut(s) 796, 1664, 2206, 2310, 2816, 2849, 3451
BsiHKCI CYCGRG 4 cut(s) 1010, 1537, 2908, 3396
BsiSI CCGG 4 cut(s) 753, 1630, 2725, 3366
BslI CCNNNNNNNGG 7 cut(s) 23, 357, 1235, 1958, 2072, 2486, 3110
BsmI GAATGC 2 cut(s) 1678, 2134
BsnI GGCC 2 cut(s) 1305, 1963
BsoBI CYCGRG 4 cut(s) 1010, 1537, 2908, 3396
Bsp119I TTCGAA 1 cut(s) 2865
Bsp1286I GDGCHC 8 cut(s) 796, 917, 1664, 2206, 2310, 2816, 2849, 3451
Bsp1407I TGTACA 1 cut(s) 2640
Bsp143I GATC 9 cut(s) 379, 1287, 1744, 3001, 3075, 3084, 3100, 3505, 3615
Bsp1720I GCTNAGC 2 cut(s) 1404, 2304
Bsp19I CCATGG 2 cut(s) 174, 1123
BspACI CCGC 6 cut(s) 1179, 1654, 1902, 1984, 2248, 3625
BspANI GGCC 2 cut(s) 1305, 1963
BspFNI CGCG 2 cut(s) 1986, 2250
BspHI TCATGA 3 cut(s) 543, 585, 885
BspLI GGNNCC 7 cut(s) 85, 1320, 1746, 2320, 3195, 3356, 3414
BspMAI CTGCAG 5 cut(s) 274, 976, 2231, 2407, 3280
BspMI ACCTGC 1 cut(s) 2220
BspPI GGATC 6 cut(s) 1295, 1739, 1752, 3079, 3095, 3513
BspT104I TTCGAA 1 cut(s) 2865
BspT107I GGYRCC 2 cut(s) 2318, 3412
BsrDI GCAATG 6 cut(s) 622, 1683, 2259, 3228, 3634, 3653
BsrGI TGTACA 1 cut(s) 2640
BssECI CCNNGG 8 cut(s) 174, 1123, 1230, 1458, 1844, 2217, 3258, 3486
BssMI GATC 9 cut(s) 379, 1287, 1744, 3001, 3075, 3084, 3100, 3505, 3615
BssSI CACGAG 1 cut(s) 3299
BssT1I CCWWGG 5 cut(s) 174, 1123, 1458, 1844, 3486
Bst2BI CACGAG 1 cut(s) 3299
Bst2UI CCWGG 4 cut(s) 963, 1232, 1316, 3191
Bst6I CTCTTC 4 cut(s) 1380, 1983, 2236, 2616
BstAPI GCANNNNNTGC 3 cut(s) 1160, 1400, 3659
BstAUI TGTACA 1 cut(s) 2640
BstBI TTCGAA 1 cut(s) 2865
BstC8I GCNNGC 8 cut(s) 613, 708, 1766, 1900, 2224, 2563, 2698, 3660
BstDSI CCRYGG 3 cut(s) 174, 1123, 2217
BstENI CCTNNNNNAGG 1 cut(s) 3108
BstF5I GGATG 9 cut(s) 64, 130, 303, 420, 530, 1084, 1114, 1375, 3133
BstFNI CGCG 2 cut(s) 1986, 2250
BstH2I RGCGCY 2 cut(s) 213, 1610
BstHHI GCGC 4 cut(s) 212, 1571, 1609, 2252
BstKTI GATC 9 cut(s) 382, 1290, 1747, 3004, 3078, 3087, 3103, 3508, 3618
BstMBI GATC 9 cut(s) 379, 1287, 1744, 3001, 3075, 3084, 3100, 3505, 3615
BstNI CCWGG 4 cut(s) 963, 1232, 1316, 3191
BstNSI RCATGY 1 cut(s) 615
BstPAI GACNNNNGTC 1 cut(s) 1637
BstSCI CCNGG 5 cut(s) 961, 1230, 1314, 2724, 3189
BstSLI GKGCMC 1 cut(s) 796
BstUI CGCG 2 cut(s) 1986, 2250
BstV2I GAAGAC 3 cut(s) 353, 562, 1809
BstX2I RGATCY 1 cut(s) 1744
BstXI CCANNNNNNTGG 1 cut(s) 1105
BstYI RGATCY 1 cut(s) 1744
Bsu36I CCTNAGG 1 cut(s) 1443
BsuI GTATCC 3 cut(s) 860, 1916, 2979
BsuRI GGCC 2 cut(s) 1305, 1963
BtgI CCRYGG 3 cut(s) 174, 1123, 2217
BtgZI GCGATG 1 cut(s) 461
BtrI CACGTC 1 cut(s) 2956
BtsCI GGATG 9 cut(s) 64, 130, 303, 420, 530, 1084, 1114, 1375, 3133
BtsIMutI CAGTG 9 cut(s) 78, 303, 955, 2022, 2536, 2673, 3078, 3451, 3522
BveI ACCTGC 1 cut(s) 2220
Cac8I GCNNGC 8 cut(s) 613, 708, 1766, 1900, 2224, 2563, 2698, 3660
CaiI CAGNNNCTG 1 cut(s) 2714
CciI TCATGA 3 cut(s) 543, 585, 885
CfoI GCGC 4 cut(s) 212, 1571, 1609, 2252
Cfr13I GGNCC 4 cut(s) 1721, 1961, 3355, 3490
CseI GACGC 3 cut(s) 1943, 2340, 2567
Csp6I GTAC 8 cut(s) 201, 816, 1199, 1949, 2031, 2515, 2551, 2641
CspCI CAANNNNNGTGG 2 cut(s) 3502, 3537
CviQI GTAC 8 cut(s) 201, 816, 1199, 1949, 2031, 2515, 2551, 2641
DpnI GATC 9 cut(s) 381, 1289, 1746, 3003, 3077, 3086, 3102, 3507, 3617
DpnII GATC 9 cut(s) 379, 1287, 1744, 3001, 3075, 3084, 3100, 3505, 3615
Eam1104I CTCTTC 4 cut(s) 1380, 1983, 2236, 2616
EarI CTCTTC 4 cut(s) 1380, 1983, 2236, 2616
Ecl136II GAGCTC 2 cut(s) 2814, 2847
Eco130I CCWWGG 5 cut(s) 174, 1123, 1458, 1844, 3486
Eco147I AGGCCT 1 cut(s) 1305
Eco24I GRGCYC 3 cut(s) 917, 2816, 2849
Eco32I GATATC 2 cut(s) 549, 2501
Eco47I GGWCC 3 cut(s) 1721, 3355, 3490
Eco47III AGCGCT 1 cut(s) 211
Eco53kI GAGCTC 2 cut(s) 2814, 2847
Eco57I CTGAAG 3 cut(s) 1404, 1550, 1929
Eco81I CCTNAGG 1 cut(s) 1443
Eco88I CYCGRG 4 cut(s) 1010, 1537, 2908, 3396
EcoICRI GAGCTC 2 cut(s) 2814, 2847
EcoNI CCTNNNNNAGG 1 cut(s) 3108
EcoO109I RGGNCCY 1 cut(s) 1961
EcoRI GAATTC 1 cut(s) 1542
EcoRII CCWGG 4 cut(s) 961, 1230, 1314, 3189
EcoRV GATATC 2 cut(s) 549, 2501
EcoT14I CCWWGG 5 cut(s) 174, 1123, 1458, 1844, 3486
EcoT22I ATGCAT 2 cut(s) 1024, 3643
EcoT38I GRGCYC 3 cut(s) 917, 2816, 2849
ErhI CCWWGG 5 cut(s) 174, 1123, 1458, 1844, 3486
FalI AAGNNNNNCTT 2 cut(s) 2090, 2122
FauNDI CATATG 3 cut(s) 1024, 1882, 3235
FbaI TGATCA 2 cut(s) 379, 3615
FblI GTMKAC 2 cut(s) 2683, 2977
FokI GGATG 9 cut(s) 51, 137, 310, 427, 537, 1091, 1121, 1382, 3140
FriOI GRGCYC 3 cut(s) 917, 2816, 2849
GlaI GCGC 4 cut(s) 211, 1570, 1608, 2251
GsuI CTGGAG 2 cut(s) 327, 417
HaeII RGCGCY 2 cut(s) 213, 1610
HaeIII GGCC 2 cut(s) 1305, 1963
HapII CCGG 4 cut(s) 753, 1630, 2725, 3366
HgaI GACGC 3 cut(s) 1943, 2340, 2567
HhaI GCGC 4 cut(s) 212, 1571, 1609, 2252
Hin6I GCGC 4 cut(s) 210, 1569, 1607, 2250
HinP1I GCGC 4 cut(s) 210, 1569, 1607, 2250
HindIII AAGCTT 1 cut(s) 741
HpaII CCGG 4 cut(s) 753, 1630, 2725, 3366
Hpy166II GTNNAC 9 cut(s) 248, 693, 794, 1332, 2275, 2684, 2978, 3317, 3371
Hpy8I GTNNAC 9 cut(s) 248, 693, 794, 1332, 2275, 2684, 2978, 3317, 3371
HpyAV CCTTC 8 cut(s) 27, 451, 1712, 2113, 2260, 2926, 3239, 3403
HpyCH4IV ACGT 2 cut(s) 2955, 2985
HpySE526I ACGT 2 cut(s) 2955, 2985
HspAI GCGC 4 cut(s) 210, 1569, 1607, 2250
Ksp22I TGATCA 2 cut(s) 379, 3615
Kzo9I GATC 9 cut(s) 379, 1287, 1744, 3001, 3075, 3084, 3100, 3505, 3615
LmnI GCTCC 6 cut(s) 641, 964, 1754, 1942, 2819, 3193
MaeII ACGT 2 cut(s) 2955, 2985
MaeIII GTNAC 3 cut(s) 301, 2475, 3113
MalI GATC 9 cut(s) 381, 1289, 1746, 3003, 3077, 3086, 3102, 3507, 3617
MboI GATC 9 cut(s) 379, 1287, 1744, 3001, 3075, 3084, 3100, 3505, 3615
MfeI CAATTG 2 cut(s) 663, 3033
MflI RGATCY 1 cut(s) 1744
MhlI GDGCHC 8 cut(s) 796, 917, 1664, 2206, 2310, 2816, 2849, 3451
MlyI GAGTC 6 cut(s) 219, 1799, 1980, 2253, 2888, 3591
MmeI TCCRAC 7 cut(s) 77, 92, 111, 749, 1150, 1300, 2350
Mph1103I ATGCAT 2 cut(s) 1024, 3643
MroXI GAANNNNTTC 2 cut(s) 593, 2525
MslI CAYNNNNRTG 2 cut(s) 2313, 3327
MspA1I CMGCKG 2 cut(s) 2045, 2768
MspI CCGG 4 cut(s) 753, 1630, 2725, 3366
MspR9I CCNGG 5 cut(s) 963, 1232, 1316, 2726, 3191
MunI CAATTG 2 cut(s) 663, 3033
Mva1269I GAATGC 2 cut(s) 1678, 2134
MvaI CCWGG 4 cut(s) 963, 1232, 1316, 3191
MvnI CGCG 2 cut(s) 1986, 2250
NciI CCSGG 1 cut(s) 2726
NcoI CCATGG 2 cut(s) 174, 1123
NdeI CATATG 3 cut(s) 1024, 1882, 3235
NdeII GATC 9 cut(s) 379, 1287, 1744, 3001, 3075, 3084, 3100, 3505, 3615
NlaIV GGNNCC 7 cut(s) 85, 1320, 1746, 2320, 3195, 3356, 3414
NmuCI GTSAC 2 cut(s) 301, 3113
NsiI ATGCAT 2 cut(s) 1024, 3643
NspI RCATGY 1 cut(s) 615
NspV TTCGAA 1 cut(s) 2865
OliI CACNNNNGTG 1 cut(s) 2313
PaeI GCATGC 1 cut(s) 615
PagI TCATGA 3 cut(s) 543, 585, 885
PceI AGGCCT 1 cut(s) 1305
PctI GAATGC 2 cut(s) 1678, 2134
PdmI GAANNNNTTC 2 cut(s) 593, 2525
PfeI GAWTC 6 cut(s) 13, 170, 2526, 3107, 3132, 3461
PfoI TCCNGGA 1 cut(s) 961
PleI GAGTC 6 cut(s) 219, 1799, 1979, 2252, 2888, 3590
PpsI GAGTC 6 cut(s) 219, 1799, 1979, 2252, 2888, 3590
PshAI GACNNNNGTC 1 cut(s) 1637
PsiI TTATAA 2 cut(s) 981, 1772
Psp124BI GAGCTC 2 cut(s) 2816, 2849
Psp6I CCWGG 4 cut(s) 961, 1230, 1314, 3189
PspGI CCWGG 4 cut(s) 961, 1230, 1314, 3189
PspN4I GGNNCC 7 cut(s) 85, 1320, 1746, 2320, 3195, 3356, 3414
PspPI GGNCC 4 cut(s) 1721, 1961, 3355, 3490
PsrI GAACNNNNNNTAC 2 cut(s) 2454, 2486
PstI CTGCAG 5 cut(s) 274, 976, 2231, 2407, 3280
PstNI CAGNNNCTG 1 cut(s) 2714
PsuI RGATCY 1 cut(s) 1744
PvuII CAGCTG 2 cut(s) 2045, 2768
RsaI GTAC 8 cut(s) 202, 817, 1200, 1950, 2032, 2516, 2552, 2642
RsaNI GTAC 8 cut(s) 201, 816, 1199, 1949, 2031, 2515, 2551, 2641
RseI CAYNNNNRTG 2 cut(s) 2313, 3327
SacI GAGCTC 2 cut(s) 2816, 2849
Sau3AI GATC 9 cut(s) 379, 1287, 1744, 3001, 3075, 3084, 3100, 3505, 3615
Sau96I GGNCC 4 cut(s) 1721, 1961, 3355, 3490
SchI GAGTC 6 cut(s) 219, 1799, 1980, 2253, 2888, 3591
ScrFI CCNGG 5 cut(s) 963, 1232, 1316, 2726, 3191
SduI GDGCHC 8 cut(s) 796, 917, 1664, 2206, 2310, 2816, 2849, 3451
SfuI TTCGAA 1 cut(s) 2865
SinI GGWCC 3 cut(s) 1721, 3355, 3490
SmiMI CAYNNNNRTG 2 cut(s) 2313, 3327
SmlI CTYRAG 2 cut(s) 1657, 2311
SmoI CTYRAG 2 cut(s) 1657, 2311
SpeI ACTAGT 1 cut(s) 1787
SphI GCATGC 1 cut(s) 615
SseBI AGGCCT 1 cut(s) 1305
SsiI CCGC 6 cut(s) 1179, 1654, 1902, 1984, 2248, 3625
SspI AATATT 3 cut(s) 1119, 1354, 2667
SstI GAGCTC 2 cut(s) 2816, 2849
StuI AGGCCT 1 cut(s) 1305
StyD4I CCNGG 5 cut(s) 961, 1230, 1314, 2724, 3189
StyI CCWWGG 5 cut(s) 174, 1123, 1458, 1844, 3486
TaiI ACGT 2 cut(s) 2958, 2988
TaqI TCGA 4 cut(s) 2810, 2865, 3068, 3508
TaqII GACCGA 1 cut(s) 3507
TatI WGTACW 4 cut(s) 815, 1198, 2514, 2640
TfiI GAWTC 6 cut(s) 13, 170, 2526, 3107, 3132, 3461
TscAI CASTG 9 cut(s) 78, 310, 955, 2022, 2536, 2680, 3078, 3451, 3522
TseFI GTSAC 2 cut(s) 301, 3113
Tsp45I GTSAC 2 cut(s) 301, 3113
TspGWI ACGGA 1 cut(s) 2511
TspRI CASTG 9 cut(s) 78, 310, 955, 2022, 2536, 2680, 3078, 3451, 3522
VneI GTGCAC 1 cut(s) 792
VpaK11BI GGWCC 3 cut(s) 1721, 3355, 3490
XagI CCTNNNNNAGG 1 cut(s) 3108
XapI RAATTY 5 cut(s) 1424, 1542, 1825, 2868, 2945
XceI RCATGY 1 cut(s) 615
XcmI CCANNNNNNNNNTGG 1 cut(s) 3365
XmiI GTMKAC 2 cut(s) 2683, 2977
XmnI GAANNNNTTC 2 cut(s) 593, 2525
Zsp2I ATGCAT 2 cut(s) 1024, 3643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.