Rw3G024160

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000116
Physical Location & Seq
Reverse (-)
30821010 .. 30826479
5470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G024160.1

Sequence Viewer

Length: 3891 bp
ATGGCTGAGGAGAATGGGGTGAATGGTGATACCAAAAATCATGAAAACCATGCAGAAGAAGAGAAAAGCTCAGCCCCCATAAATAGGGATCAGCCAGGCTCATCAGATAGCAATGGAGATGAGAAAGTTGAAAAGATTCCTTTCTCCAAGCTATTCTCCTTTGCAGATAAGACTGATGTTATCTTGATGGTTGTCGGCACGATTGGTGCCATTGGGAATGGTTCTTGTATGCCCCTCATGACGGTATTGTTTGGGGAGATGATAGATTCTTTTGGATCTAATCAAAATTCAGACATAGTCTCAGTTGTTTCAAAGGTCTCTCTGAAATTTGTTTACCTGGCTGTGGGAGCAGCTGTGGCAGCATTTCTCCAGGTGTCTTGCTGGATGGTCACAGGGGAAAGACAAGCTGCAAGAATTAGAGGTTTGTATTTGAAAACAATATTGAGACAAGATGTTGCTTTCTTCGATATGGAAACAAACACTGGAGAGGTTGTTGGGAGAATGTCTGGTGACACTGTTCTCATTCAAGATGCCATGGGGGAGAAGGTTGGAAAATTCTTACAGCTGCTTGCAACATTCATTGGAGGGTTTATAATAGCATTCATCAAAGGGTGGCTTCTCACTCTTGTCATGCTATCCTCTATTCCTTTGCTTGTGGCATCTGGTGCAACGATGTCCATCATTATAACCAAGATGGCATCCCGTGGACAAACTGCTTACGCAAAAGCAGCAAATGTAGTTGAAAAACAATTGGGTCCATTAGAACCGTATGTTGCATCATTTACTGGCGAGAGGGAAGCTATAACTAGTTATAACAAATATCTCGTAGATGCTTACAAATCAGGTGTTCATGAAGGTTCAGCTGCTGGAATAGGTCTTGGCTTGGTTATGTGTGTTGTGTTCAGCACATATGCCTTGGCTGTATGGTTTGGTTCAAAGATGATTAGGGAAAATGGATATACTGGGGGTGAAGTACTGAATGTGATTGTTGCTGTGTTGACTGGATCTATGTCTTTAGGGCAGACATCACCATGCATGAGTGCATTCGCTGCTGGTCAAGCTGCAGCTTATAAGATGTTTCAGACTATTAGTAGGAAGCCAGAGATAGATGCTTATGATGAAAGGGGAAAGACTTTGGGTGACATTTCGGGAGACATAGAGTTGAGAGATGTTTATTTCAGCTATCCAGCCAGACCGGATGAGCAAATATTTGACGGGTTCTCTCTTTGTATCCCTAGTGGCACAACTGCTGCTTTGGTTGGACAAAGTGGAAGTGGCAAGTCAACAGTCATCAGTCTGATAGAGAGATTTTATGATCCGCAAGCTGGTGAAGTTTTAATAGATGGCATAAACCTAAAAGAATTTCAACTTAAATGGATTCGGAGTAAAATTGGTCTTGTCAGTCAGGAACCTGTCCTGTTTGCATCCAGCATTAAGGAAAATATTGCCTATGGAAAGGATGGTGCTACCACTGAAGAGATACAAGCAGCAGCTGAGCTTGCAAATGCAGCTAAATTTGTTGACAAATTGCCTCAGGGCCTTGACAGCTTGGTCGGTGAGCATGGAACTCAGCTGTCTGGTGGGCAGAAGCAGAGAATTGCTATTGCAAGAGCAATTTTGAAAGACCCAAGGATTTTACTTCTGGATGAAGCTACAAGTGCACTTGATGCAGAATCTGAGAGGGTAGTTCAAGAAGCATTGGATAGGATTATGGTTAACCGAACTACTGTTGTTGTCGCTCATCGTTTGAGTACAGTGAGGAATGCAGACATGATTGCTGTCATTCATAAAGGAAAGATGGTTGAAAAAGGTTCGCACTTGAATTTACTCAAAGATCCTGAGGGAGCTTACTCTCAGCTTATACGCTTGCAAGAAGTAAACAAAGAGTCAGAACAAACGTCAGAAGACCAAAACAAACCTGAAATAACTCTGGCATCTCTTAGACAGTCAAGTCAAAGAACATCAAGTCAAAGACTTTCGTTTGCACGATCCTTAAGTAGAAATTCCTCCGTAGGAAATAGCAGCCGTCACTCATATTCAGTGGCATTTGGTTTACCCACTGGACTTGGAGGCATTGGTGTCCAAGATGCTGCATATGAAGAAACAGAACTAGCACCAGAGCAACCTCCAAAGGTCTCACTCCGCCGCATTGCTGCCCTGAACAAACCTGAGATTCCAGTTCTTATTATTGGAACTATAGCTGCAATCATCAATGGTGTTATACTTCCAATATTCGGTGTGCTCATTTCCAGGGTTATAAAGACTTTCTATGAACCACCTCATCAACAGAAGAAAGATGCAGCATTTTGGGCAATAATTTTTATGATCCTTGGTCTGATATCATTCGTGGCGATCCCAGCACGAGGATATTTCTTTTCAGTAGCTGGCAGCAAGTTAATTCAGCGTATCAGATTATTGTGTTTTGAGAGAGTGGTTCACATGGAGGTTGGGTGGTTTGATGAGCCTGAGAACTCAAGTGGTGCAATTGGTGCAAGGCTCTCAGCAGATGCAGCGTCGGTGAGGGCCCTAGTCGGAGACGCTCTAGCTCAGATGGTTCAAAACATGGCAGCTGCAGTTGCAGGTTTGGTCATTGCTTATATCGCATGTTGGCAGCTGGCATTTATCATCCTCGCACTGCTTCCTCTCATTGCAGTTAACGGATATGTACAAATAAAGTTCATGAAAGGATTCAGCGCAGATGCAAAGATGATGTATGAGGAGGCAAGCCAAGTTGCTAATGACGCAGTTGGAAGTATAAGAACAGTTGCTTCTTTCTGTGCTGAAGAGAAGGTAATGGAACTATACAGAAGGAAATGTGAAGGCCCTATGAAGACAGGGATAAGACAAGGCTTGATCAGTGGTATAGGATTTGGGGTATCTTTCTTCTTTTTGTTTTGTGTCTACGCAACCAGTTTCTATGCTGGAGCTCAACTCGTGAAGGCTGGCAAAACAACATTTACAGACGTTTTCCAAGTTTTCTTTGCTCTGACCATGGCAGCTACCGGAATTTCTCAATCAAGCTCCGTTGGTCCCGATTCTGGTAAAGCCAAAACTTCTGCTGCTTCCATATTTGCAATAATAGACCGGCCATCAAAGATAGACCCAAGTAACGAGTCTGGTACGAAAATTGATGGTGGTGTGAAGGGAGAGATTGAGCTTCGCCATGTAAGCTTTAAGTATCCATCTAGGCCAGAAACACCTATTTTCCGAGACCTCAATTTAACTATCCGCTCTGGCAAGACGGTTGCCCTGGTCGGAGAAAGTGGGAGCGGAAAATCAACTGTTGTTGCATTGTTGCAAAGATTTTATGATCCGGATTCAGGTCATATCACACTTGATGGAATTGAACTTGGCAACTACAACTTGAAATGGTTGAGGCAGCAGATGGGACTCGTAAGCCAAGAACCTGTTTTATTTAATGACACCATTCGCGCCAACATTGCGTATGGAAAGGAAGAAACTGCAACTGAGGCAGAAATTATAGCTGCATCAGAGTTGGCCAATGCCCACAAGTTCATCAGTAGCCTACACCAGGGCTATGATACCGTAGTAGGAGAACGAGGAATCCAATTATCCGGGGGGCAGAAGCAACGTGTAGCCATTGCGCGTGCTATAATCAAGAGCCCCAAGATATTACTGTTGGATGAAGCTACGAGTGCACTTGATGCCGAATCTGAGAGAGTGGTTCAAGATGCATTAGACCGAGTCATGGTGAACCGGACAACAGTGGTGGTGGCTCATAGACTATCCACAATCAAGAATGCTGATGTGATTGCAGTGGTTAAAAATGGAGTTATAGTAGAGAAAGGGAAGCATGACAATTTGATTAATATCACCGATGGGTTTTATGCATCCCTAGTTGCTCTTCACATGAGCTCTTCAACTGCATGA

Protein Analysis

1296

Amino Acids

139.64

Weight (kDa)

6.27

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 64 - 337 1.7e-53 ABC transporter transmembrane region
ABC_tran PF00005 405 - 553 3.1e-33 ABC transporter
ABC_membrane PF00664 728 - 999 2.9e-59 ABC transporter transmembrane region
ABC_tran PF00005 1070 - 1219 5.3e-35 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02520 AT1G02520 AT1G02520 AT1G02530 AT1G02530 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT3G62150 AT3G62150 AT3G62150 AT4G01820 AT4G01820 AT4G01830 AT4G01830
fragaria_vesca FvH4_1g26280 FvH4_4g21370 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21381 FvH4_4g21600 FvH4_4g21600 FvH4_4g21600 FvH4_4g21620 FvH4_4g21621 FvH4_4g21622 FvH4_6g00641 FvH4_6g00650
malus_domestica MD09G1290100.v1.1 MD13G1138600.v1.1 MD13G1138700.v1.1 MD13G1139100.v1.1 MD16G1134500.v1.1 MD16G1134700.v1.1 MD16G1135000.v1.1 MD16G1135100.v1.1 MD17G1284300.v1.1 MD17G1284700.v1.1
prunus_persica Prupe.1G103800_v2.0.a1 Prupe.1G103800_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1
pyrus_communis pycom04g08750 pycom09g19770 pycom09g19790 pycom13g09140 pycom16g11900 pycom16g11920 pycom16g11930 pycom17g28030
rosa_chinensis RchiOBHm_Chr2g0157341 RchiOBHm_Chr3g0448061 RchiOBHm_Chr3g0485931 RchiOBHm_Chr3g0485961 RchiOBHm_Chr4g0427151 RchiOBHm_Chr4g0427161 RchiOBHm_Chr4g0427211 RchiOBHm_Chr4g0427241 RchiOBHm_Chr4g0427271 RchiOBHm_Chr4g0427711 RchiOBHm_Chr4g0427721 RchiOBHm_Chr4g0427771 RchiOBHm_Chr4g0427781 RchiOBHm_Chr4g0427791 RchiOBHm_Chr4g0427851 RchiOBHm_Chr4g0427861 RchiOBHm_Chr4g0446561 RchiOBHm_Chr5g0081601 RchiOBHm_Chr6g0278251 RchiOBHm_Chr6g0303241
rosa_laevigata RLG00000007206 RLG00000007207 RLG00000007209 RLG00000007210 RLG00000007211 RLG00000007212 RLG00000007214 RLG00000007255 RLG00000007256 RLG00000007257 RLG00000011097 RLG00000019257 RLG00000023121 RLG00000025948 RLG00000027246 RLG00000029989
rosa_multiflora Rmu_co8076196.1_g000001 Rmu_co8517461.1_g000001 Rmu_sc0000066.1_g000018 Rmu_sc0000066.1_g000024 Rmu_sc0000066.1_g000033 Rmu_sc0000066.1_g000047 Rmu_sc0000066.1_g000048 Rmu_sc0001651.1_g000024 Rmu_sc0002816.1_g000003 Rmu_sc0003337.1_g000015 Rmu_sc0003444.1_g000015 Rmu_sc0003813.1_g000004 Rmu_sc0004876.1_g000001 Rmu_sc0005969.1_g000025 Rmu_sc0008650.1_g000006 Rmu_sc0008855.1_g000001 Rmu_sc0009165.1_g000001 Rmu_sc0009165.1_g000002 Rmu_sc0010010.1_g000001 Rmu_sc0010010.1_g000005 Rmu_sc0036078.1_g000001
rosa_roxburghii Rroxscaffold_2G00130200 Rroxscaffold_5G00367590 Rroxscaffold_5G00368970 Rroxscaffold_5G00368990 Rroxscaffold_5G00369340 Rroxscaffold_5G00369350 Rroxscaffold_5G00369380 Rroxscaffold_5G00369390 Rroxscaffold_5G00369440 Rroxscaffold_5G00369450 Rroxscaffold_5G00369500 Rroxscaffold_5G00369510 Rroxscaffold_6G00396190 Rroxscaffold_6G00426220 Rroxscaffold_6G00427790 Rroxscaffold_7G00164940
rosa_rugosa Rorug01G0160800.1 Rorug02G0341400 Rorug02G0461800 Rorug02G0608000 Rorug03G0222700 Rorug03G0222800 Rorug03G0222900 Rorug04G0216400 Rorug04G0216400 Rorug04G0220100 Rorug04G0220100 Rorug04G0220100 Rorug05G0181800 Rorug05G0409700
rosa_samantha Rh1BG177300 Rh1DG206800 Rh2AG590600 Rh2BG473400 Rh2DG482300 Rh3AG007500 Rh3AG272400 Rh3BG007200 Rh3BG307000 Rh3DG301600 Rh3DG301900 Rh4AG272300 Rh4AG272400 Rh4AG272700 Rh4AG275900 Rh4AG276100 Rh4AG276200 Rh4AG276500 Rh4AG276600 Rh4AG276700 Rh4AG277000 Rh4BG281900 Rh4BG282200 Rh4CG292900 Rh4CG293300 Rh4CG293400 Rh4CG293600 Rh4CG297400 Rh4CG297500 Rh4CG297900 Rh4CG298000 Rh4CG298300 Rh4CG298400 Rh4DG165900 Rh5BG011400 Rh5DG092900 Rh6AG434000 Rh6CG153300 Rh6CG391700 Rh7BG333600
rosa_wichuraiana Rw0G005570 Rw0G005590 Rw0G011850 Rw0G015530 Rw0G017350 Rw0G019580 Rw3G000600 Rw3G024160 Rw4G023620 Rw4G023640 Rw4G023660 Rw4G023670 Rw4G023950 Rw4G023960 Rw4G024050 Rw4G024060 Rw5G048020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 593, 686, 813, 1071, 2258
AasI GACNNNNNNGTC 2 cut(s) 1550, 1950
Acc36I ACCTGC 1 cut(s) 2570
AccB1I GGYRCC 1 cut(s) 206
AccBSI CCGCTC 2 cut(s) 3231, 3270
AccI GTMKAC 1 cut(s) 2901
AccII CGCG 2 cut(s) 3432, 3607
AccIII TCCGGA 1 cut(s) 3313
AciI CCGC 5 cut(s) 1319, 2143, 2146, 3229, 3270
AclWI GGATC 9 cut(s) 96, 283, 1012, 1310, 1829, 1983, 2320, 2347, 3305
AcoI YGGCCR 2 cut(s) 3086, 3498
AcsI RAATTY 8 cut(s) 286, 326, 554, 1361, 1514, 1822, 2002, 3006
AcuI CTGAAG 2 cut(s) 1494, 2802
AfaI GTAC 4 cut(s) 975, 1753, 2667, 3121
AflII CTTAAG 1 cut(s) 1993
AflIII ACRYGT 1 cut(s) 3592
AhlI ACTAGT 1 cut(s) 806
AjnI CCWGG 6 cut(s) 94, 336, 369, 2249, 3249, 3531
AloI GAACNNNNNNTCC 2 cut(s) 3549, 3581
Alw21I GWGCWC 5 cut(s) 1663, 2244, 2929, 3661, 3878
Alw26I GTCTC 7 cut(s) 304, 322, 439, 1146, 2140, 2529, 3204
Alw44I GTGCAC 2 cut(s) 1659, 3657
AlwI GGATC 9 cut(s) 96, 283, 1012, 1310, 1829, 1983, 2320, 2347, 3305
AlwNI CAGNNNCTG 4 cut(s) 866, 1493, 1676, 2384
Aor13HI TCCGGA 1 cut(s) 3313
AoxI GGCC 6 cut(s) 1537, 2523, 2821, 3086, 3188, 3498
ApaI GGGCCC 1 cut(s) 2527
ApaLI GTGCAC 2 cut(s) 1659, 3657
ApoI RAATTY 8 cut(s) 286, 326, 554, 1361, 1514, 1822, 2002, 3006
AseI ATTAAT 1 cut(s) 3828
Asp700I GAANNNNTTC 2 cut(s) 135, 2687
AspLEI GCGC 3 cut(s) 2696, 3434, 3607
AspS9I GGNCC 6 cut(s) 755, 1537, 2523, 2524, 2822, 3029
AsuC2I CCSGG 1 cut(s) 3577
AvaII GGWCC 2 cut(s) 755, 3029
AxyI CCTNAGG 2 cut(s) 1533, 1839
BaeGI GKGCMC 3 cut(s) 1663, 2527, 3661
BaeI ACNNNNGTAYC 2 cut(s) 3534, 3567
BalI TGGCCA 1 cut(s) 3500
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 4 cut(s) 2527, 2929, 3626, 3878
BauI CACGAG 2 cut(s) 2361, 2933
BbsI GAAGAC 2 cut(s) 1911, 2837
Bbv12I GWGCWC 5 cut(s) 1663, 2244, 2929, 3661, 3878
BbvCI CCTCAGC 1 cut(s) 6
BceAI ACGGC 1 cut(s) 2010
BciT130I CCWGG 6 cut(s) 96, 338, 371, 2251, 3251, 3533
BciVI GTATCC 2 cut(s) 1241, 3189
BclI TGATCA 1 cut(s) 2853
BcnI CCSGG 1 cut(s) 3577
BcoDI GTCTC 7 cut(s) 304, 322, 439, 1146, 2140, 2529, 3204
BcuI ACTAGT 1 cut(s) 806
BfaI CTAG 7 cut(s) 807, 1236, 2111, 2528, 2543, 3186, 3857
BfmI CTRYAG 3 cut(s) 1062, 2196, 2571
BfrI CTTAAG 1 cut(s) 1993
BfuAI ACCTGC 1 cut(s) 2570
BfuI GTATCC 2 cut(s) 1241, 3189
BlpI GCTNAGC 2 cut(s) 70, 1494
BmcAI AGTACT 1 cut(s) 975
Bme1390I CCNGG 7 cut(s) 96, 338, 371, 2251, 3251, 3533, 3577
Bme18I GGWCC 2 cut(s) 755, 3029
BmgT120I GGNCC 6 cut(s) 755, 1537, 2523, 2524, 2822, 3029
BmiI GGNNCC 5 cut(s) 208, 756, 1410, 2525, 3031
BmrFI CCNGG 7 cut(s) 96, 338, 371, 2251, 3251, 3533, 3577
BmrI ACTGGG 1 cut(s) 972
BmuI ACTGGG 1 cut(s) 972
BpiI GAAGAC 2 cut(s) 1911, 2837
BplI GAGNNNNNCTC 4 cut(s) 53, 85, 2916, 2948
BpmI CTGGAG 3 cut(s) 353, 504, 2943
Bpu10I CCTNAGC 1 cut(s) 6
Bpu1102I GCTNAGC 2 cut(s) 70, 1494
BpuEI CTTGAG 1 cut(s) 2458
BpuMI CCSGG 1 cut(s) 3577
BsaBI GATNNNNATC 2 cut(s) 677, 3830
BsaI GGTCTC 3 cut(s) 322, 2140, 3204
BsaWI WCCGGW 4 cut(s) 1195, 3002, 3313, 3717
BsaXI ACNNNNNCTCC 8 cut(s) 339, 369, 477, 507, 2435, 2465, 3783, 3813
Bse118I RCCGGY 1 cut(s) 3084
Bse1I ACTGG 7 cut(s) 487, 790, 967, 1006, 2065, 2177, 2910
Bse21I CCTNAGG 2 cut(s) 1533, 1839
Bse3DI GCAATG 6 cut(s) 118, 2148, 2589, 2646, 3438, 3600
Bse8I GATNNNNATC 2 cut(s) 677, 3830
BseAI TCCGGA 1 cut(s) 3313
BseBI CCWGG 6 cut(s) 96, 338, 371, 2251, 3251, 3533
BseGI GGATG 9 cut(s) 390, 698, 1204, 1424, 1465, 1651, 2625, 3649, 3851
BseJI GATNNNNATC 2 cut(s) 677, 3830
BseMI GCAATG 6 cut(s) 118, 2148, 2589, 2646, 3438, 3600
BseNI ACTGG 7 cut(s) 487, 790, 967, 1006, 2065, 2177, 2910
BseRI GAGGAG 2 cut(s) 23, 2732
BseSI GKGCMC 3 cut(s) 1663, 2527, 3661
BseYI CCCAGC 1 cut(s) 2356
Bsh1236I CGCG 2 cut(s) 3432, 3607
BshFI GGCC 6 cut(s) 1539, 2525, 2823, 3088, 3190, 3500
BshNI GGYRCC 1 cut(s) 206
BsiHKAI GWGCWC 5 cut(s) 1663, 2244, 2929, 3661, 3878
BsiSI CCGG 6 cut(s) 1196, 3003, 3085, 3314, 3576, 3718
BslFI GGGAC 2 cut(s) 3015, 3402
BsmAI GTCTC 7 cut(s) 304, 322, 439, 1146, 2140, 2529, 3204
BsmBI CGTCTC 1 cut(s) 2529
BsmFI GGGAC 2 cut(s) 3015, 3402
BsmI GAATGC 4 cut(s) 599, 1043, 1768, 3766
BsnI GGCC 6 cut(s) 1539, 2525, 2823, 3088, 3190, 3500
Bso31I GGTCTC 3 cut(s) 322, 2140, 3204
Bsp120I GGGCCC 1 cut(s) 2523
Bsp1286I GDGCHC 7 cut(s) 1663, 2244, 2527, 2929, 3626, 3661, 3878
Bsp13I TCCGGA 1 cut(s) 3313
Bsp1407I TGTACA 1 cut(s) 2665
Bsp1720I GCTNAGC 2 cut(s) 70, 1494
Bsp19I CCATGG 2 cut(s) 534, 2991
BspACI CCGC 5 cut(s) 1319, 2143, 2146, 3229, 3270
BspANI GGCC 6 cut(s) 1539, 2525, 2823, 3088, 3190, 3500
BspEI TCCGGA 1 cut(s) 3313
BspFNI CGCG 2 cut(s) 3432, 3607
BspHI TCATGA 4 cut(s) 40, 237, 850, 2679
BspLI GGNNCC 5 cut(s) 208, 756, 1410, 2525, 3031
BspMAI CTGCAG 2 cut(s) 1066, 2575
BspMI ACCTGC 1 cut(s) 2570
BspPI GGATC 9 cut(s) 96, 283, 1012, 1310, 1829, 1983, 2320, 2347, 3305
BspQI GCTCTTC 2 cut(s) 3870, 3883
BspT107I GGYRCC 1 cut(s) 206
BspTI CTTAAG 1 cut(s) 1993
BspTNI GGTCTC 3 cut(s) 322, 2140, 3204
BsrBI CCGCTC 2 cut(s) 3231, 3270
BsrDI GCAATG 6 cut(s) 118, 2148, 2589, 2646, 3438, 3600
BsrFI RCCGGY 1 cut(s) 3084
BsrGI TGTACA 1 cut(s) 2665
BsrI ACTGG 7 cut(s) 487, 790, 967, 1006, 2065, 2177, 2910
BssAI RCCGGY 1 cut(s) 3084
BssSI CACGAG 2 cut(s) 2361, 2933
BssT1I CCWWGG 5 cut(s) 534, 915, 1628, 2329, 2991
Bst2BI CACGAG 2 cut(s) 2361, 2933
Bst2UI CCWGG 6 cut(s) 96, 338, 371, 2251, 3251, 3533
Bst6I CTCTTC 5 cut(s) 54, 1470, 2778, 3870, 3883
BstAFI CTTAAG 1 cut(s) 1993
BstAPI GCANNNNNTGC 5 cut(s) 665, 1049, 1667, 2489, 3665
BstAUI TGTACA 1 cut(s) 2665
BstC8I GCNNGC 9 cut(s) 570, 1323, 1500, 1868, 2386, 2616, 2725, 2944, 3609
BstDSI CCRYGG 3 cut(s) 534, 703, 2991
BstENI CCTNNNNNAGG 1 cut(s) 3530
BstF5I GGATG 9 cut(s) 390, 698, 1204, 1424, 1465, 1651, 2625, 3649, 3851
BstFNI CGCG 2 cut(s) 3432, 3607
BstHHI GCGC 3 cut(s) 2696, 3434, 3607
BstMAI GTCTC 7 cut(s) 304, 322, 439, 1146, 2140, 2529, 3204
BstNI CCWGG 6 cut(s) 96, 338, 371, 2251, 3251, 3533
BstNSI RCATGY 1 cut(s) 2607
BstSCI CCNGG 7 cut(s) 94, 336, 369, 2249, 3249, 3531, 3575
BstSFI CTRYAG 3 cut(s) 1062, 2196, 2571
BstSLI GKGCMC 3 cut(s) 1663, 2527, 3661
BstUI CGCG 2 cut(s) 3432, 3607
BstV2I GAAGAC 2 cut(s) 1911, 2837
BstX2I RGATCY 3 cut(s) 275, 1004, 1834
BstYI RGATCY 3 cut(s) 275, 1004, 1834
Bsu36I CCTNAGG 2 cut(s) 1533, 1839
BsuI GTATCC 2 cut(s) 1241, 3189
BsuRI GGCC 6 cut(s) 1539, 2525, 2823, 3088, 3190, 3500
BtgI CCRYGG 3 cut(s) 534, 703, 2991
BtsCI GGATG 9 cut(s) 390, 698, 1204, 1424, 1465, 1651, 2625, 3649, 3851
BtsI GCAGTG 2 cut(s) 2633, 3783
BveI ACCTGC 1 cut(s) 2570
Cac8I GCNNGC 9 cut(s) 570, 1323, 1500, 1868, 2386, 2616, 2725, 2944, 3609
CaiI CAGNNNCTG 4 cut(s) 866, 1493, 1676, 2384
CciI TCATGA 4 cut(s) 40, 237, 850, 2679
CfoI GCGC 3 cut(s) 2696, 3434, 3607
Cfr10I RCCGGY 1 cut(s) 3084
Cfr13I GGNCC 6 cut(s) 755, 1537, 2523, 2524, 2822, 3029
CseI GACGC 3 cut(s) 2502, 2546, 2750
Csp6I GTAC 4 cut(s) 974, 1752, 2666, 3120
CspCI CAANNNNNGTGG 4 cut(s) 2047, 2082, 3711, 3746
CviQI GTAC 4 cut(s) 974, 1752, 2666, 3120
DrdI GACNNNNNNGTC 2 cut(s) 1550, 1950
DseDI GACNNNNNNGTC 2 cut(s) 1550, 1950
EaeI YGGCCR 2 cut(s) 3086, 3498
Eam1104I CTCTTC 5 cut(s) 54, 1470, 2778, 3870, 3883
EarI CTCTTC 5 cut(s) 54, 1470, 2778, 3870, 3883
EciI GGCGGA 1 cut(s) 2132
Ecl136II GAGCTC 2 cut(s) 2927, 3876
Eco130I CCWWGG 5 cut(s) 534, 915, 1628, 2329, 2991
Eco24I GRGCYC 4 cut(s) 2527, 2929, 3626, 3878
Eco31I GGTCTC 3 cut(s) 322, 2140, 3204
Eco32I GATATC 1 cut(s) 2340
Eco47I GGWCC 2 cut(s) 755, 3029
Eco53kI GAGCTC 2 cut(s) 2927, 3876
Eco57I CTGAAG 2 cut(s) 1494, 2802
Eco81I CCTNAGG 2 cut(s) 1533, 1839
EcoICRI GAGCTC 2 cut(s) 2927, 3876
EcoNI CCTNNNNNAGG 1 cut(s) 3530
EcoO109I RGGNCCY 4 cut(s) 1537, 2523, 2524, 2822
EcoRII CCWGG 6 cut(s) 94, 336, 369, 2249, 3249, 3531
EcoRV GATATC 1 cut(s) 2340
EcoT14I CCWWGG 5 cut(s) 534, 915, 1628, 2329, 2991
EcoT22I ATGCAT 3 cut(s) 1037, 3697, 3853
EcoT38I GRGCYC 4 cut(s) 2527, 2929, 3626, 3878
ErhI CCWWGG 5 cut(s) 534, 915, 1628, 2329, 2991
Esp3I CGTCTC 1 cut(s) 2529
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FaqI GGGAC 2 cut(s) 3015, 3402
FauNDI CATATG 2 cut(s) 910, 2095
FbaI TGATCA 1 cut(s) 2853
FblI GTMKAC 1 cut(s) 2901
FokI GGATG 9 cut(s) 397, 685, 1211, 1411, 1472, 1658, 2612, 3656, 3838
FriOI GRGCYC 4 cut(s) 2527, 2929, 3626, 3878
FspBI CTAG 7 cut(s) 807, 1236, 2111, 2528, 2543, 3186, 3857
GlaI GCGC 3 cut(s) 2695, 3433, 3606
GsaI CCCAGC 1 cut(s) 2360
GsuI CTGGAG 3 cut(s) 353, 504, 2943
HaeIII GGCC 6 cut(s) 1539, 2525, 2823, 3088, 3190, 3500
HapII CCGG 6 cut(s) 1196, 3003, 3085, 3314, 3576, 3718
HgaI GACGC 3 cut(s) 2502, 2546, 2750
HhaI GCGC 3 cut(s) 2696, 3434, 3607
Hin6I GCGC 3 cut(s) 2694, 3432, 3605
HinP1I GCGC 3 cut(s) 2694, 3432, 3605
HincII GTYRAC 5 cut(s) 999, 1284, 1522, 1717, 2656
HindII GTYRAC 5 cut(s) 999, 1284, 1522, 1717, 2656
HindIII AAGCTT 1 cut(s) 3169
HpaI GTTAAC 2 cut(s) 1717, 2656
HpaII CCGG 6 cut(s) 1196, 3003, 3085, 3314, 3576, 3718
Hpy99I CGWCG 1 cut(s) 2518
HpyAV CCTTC 7 cut(s) 538, 848, 2782, 2802, 2813, 2932, 3136
HpyCH4IV ACGT 3 cut(s) 1898, 2964, 3592
HpySE526I ACGT 3 cut(s) 1898, 2964, 3592
HspAI GCGC 3 cut(s) 2694, 3432, 3605
Kpn2I TCCGGA 1 cut(s) 3313
Ksp22I TGATCA 1 cut(s) 2853
KspAI GTTAAC 2 cut(s) 1717, 2656
LguI GCTCTTC 2 cut(s) 3870, 3883
LmnI GCTCC 5 cut(s) 347, 1844, 2924, 3026, 3267
MaeI CTAG 7 cut(s) 807, 1236, 2111, 2528, 2543, 3186, 3857
MaeII ACGT 3 cut(s) 1898, 2964, 3592
MaeIII GTNAC 5 cut(s) 388, 509, 1139, 2027, 3107
MbiI CCGCTC 2 cut(s) 3231, 3270
MfeI CAATTG 2 cut(s) 749, 2484
MflI RGATCY 3 cut(s) 275, 1004, 1834
MhlI GDGCHC 7 cut(s) 1663, 2244, 2527, 2929, 3626, 3661, 3878
MlsI TGGCCA 1 cut(s) 3500
MluNI TGGCCA 1 cut(s) 3500
MlyI GAGTC 4 cut(s) 1895, 3122, 3384, 3714
MmeI TCCRAC 6 cut(s) 529, 1240, 2512, 2728, 3235, 3621
Mox20I TGGCCA 1 cut(s) 3500
Mph1103I ATGCAT 3 cut(s) 1037, 3697, 3853
MroI TCCGGA 1 cut(s) 3313
MroXI GAANNNNTTC 2 cut(s) 135, 2687
MscI TGGCCA 1 cut(s) 3500
MslI CAYNNNNRTG 1 cut(s) 1030
Msp20I TGGCCA 1 cut(s) 3500
MspA1I CMGCKG 7 cut(s) 353, 565, 863, 1493, 1573, 2570, 2614
MspCI CTTAAG 1 cut(s) 1993
MspI CCGG 6 cut(s) 1196, 3003, 3085, 3314, 3576, 3718
MspR9I CCNGG 7 cut(s) 96, 338, 371, 2251, 3251, 3533, 3577
MunI CAATTG 2 cut(s) 749, 2484
Mva1269I GAATGC 4 cut(s) 599, 1043, 1768, 3766
MvaI CCWGG 6 cut(s) 96, 338, 371, 2251, 3251, 3533
MvnI CGCG 2 cut(s) 3432, 3607
NciI CCSGG 1 cut(s) 3577
NcoI CCATGG 2 cut(s) 534, 2991
NdeI CATATG 2 cut(s) 910, 2095
NlaIV GGNNCC 5 cut(s) 208, 756, 1410, 2525, 3031
NmuCI GTSAC 4 cut(s) 388, 509, 1139, 2027
NsiI ATGCAT 3 cut(s) 1037, 3697, 3853
NspI RCATGY 1 cut(s) 2607
PagI TCATGA 4 cut(s) 40, 237, 850, 2679
PciSI GCTCTTC 2 cut(s) 3870, 3883
PctI GAATGC 4 cut(s) 599, 1043, 1768, 3766
PdmI GAANNNNTTC 2 cut(s) 135, 2687
PflFI GACNNNGTC 2 cut(s) 296, 3704
PleI GAGTC 4 cut(s) 1894, 3121, 3384, 3713
PpsI GAGTC 4 cut(s) 1894, 3121, 3384, 3713
PshBI ATTAAT 1 cut(s) 3828
PsiI TTATAA 5 cut(s) 593, 686, 813, 1071, 2258
Psp124BI GAGCTC 2 cut(s) 2929, 3878
Psp6I CCWGG 6 cut(s) 94, 336, 369, 2249, 3249, 3531
PspFI CCCAGC 1 cut(s) 2356
PspGI CCWGG 6 cut(s) 94, 336, 369, 2249, 3249, 3531
PspN4I GGNNCC 5 cut(s) 208, 756, 1410, 2525, 3031
PspOMI GGGCCC 1 cut(s) 2523
PspPI GGNCC 6 cut(s) 755, 1537, 2523, 2524, 2822, 3029
PstI CTGCAG 2 cut(s) 1066, 2575
PstNI CAGNNNCTG 4 cut(s) 866, 1493, 1676, 2384
PsuI RGATCY 3 cut(s) 275, 1004, 1834
PsyI GACNNNGTC 2 cut(s) 296, 3704
PvuII CAGCTG 7 cut(s) 353, 565, 863, 1493, 1573, 2570, 2614
RsaI GTAC 4 cut(s) 975, 1753, 2667, 3121
RsaNI GTAC 4 cut(s) 974, 1752, 2666, 3120
RseI CAYNNNNRTG 1 cut(s) 1030
SacI GAGCTC 2 cut(s) 2929, 3878
SapI GCTCTTC 2 cut(s) 3870, 3883
Sau96I GGNCC 6 cut(s) 755, 1537, 2523, 2524, 2822, 3029
ScaI AGTACT 1 cut(s) 975
SchI GAGTC 4 cut(s) 1895, 3122, 3384, 3714
ScrFI CCNGG 7 cut(s) 96, 338, 371, 2251, 3251, 3533, 3577
SduI GDGCHC 7 cut(s) 1663, 2244, 2527, 2929, 3626, 3661, 3878
SfcI CTRYAG 3 cut(s) 1062, 2196, 2571
SinI GGWCC 2 cut(s) 755, 3029
SmiMI CAYNNNNRTG 1 cut(s) 1030
SmlI CTYRAG 2 cut(s) 1993, 2473
SmoI CTYRAG 2 cut(s) 1993, 2473
SpeI ACTAGT 1 cut(s) 806
SsiI CCGC 5 cut(s) 1319, 2143, 2146, 3229, 3270
SspI AATATT 4 cut(s) 441, 1209, 1444, 2232
SspMI CTAG 7 cut(s) 807, 1236, 2111, 2528, 2543, 3186, 3857
SstI GAGCTC 2 cut(s) 2929, 3878
StyD4I CCNGG 7 cut(s) 94, 336, 369, 2249, 3249, 3531, 3575
StyI CCWWGG 5 cut(s) 534, 915, 1628, 2329, 2991
TaiI ACGT 3 cut(s) 1901, 2967, 3595
TaqI TCGA 1 cut(s) 465
TaqII GACCGA 1 cut(s) 3717
TatI WGTACW 3 cut(s) 973, 1751, 2665
TauI GCSGC 1 cut(s) 2148
TseFI GTSAC 4 cut(s) 388, 509, 1139, 2027
Tsp45I GTSAC 4 cut(s) 388, 509, 1139, 2027
TspGWI ACGGA 3 cut(s) 1999, 2673, 3013
Tth111I GACNNNGTC 2 cut(s) 296, 3704
Vha464I CTTAAG 1 cut(s) 1993
VneI GTGCAC 2 cut(s) 1659, 3657
VpaK11BI GGWCC 2 cut(s) 755, 3029
VspI ATTAAT 1 cut(s) 3828
XagI CCTNNNNNAGG 1 cut(s) 3530
XapI RAATTY 8 cut(s) 286, 326, 554, 1361, 1514, 1822, 2002, 3006
XceI RCATGY 1 cut(s) 2607
XmiI GTMKAC 1 cut(s) 2901
XmnI GAANNNNTTC 2 cut(s) 135, 2687
XspI CTAG 7 cut(s) 807, 1236, 2111, 2528, 2543, 3186, 3857
ZrmI AGTACT 1 cut(s) 975
Zsp2I ATGCAT 3 cut(s) 1037, 3697, 3853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.