Rh4AG272400

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000116
Physical Location & Seq
Reverse (-)
58813873 .. 58817383
3511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG272400.1

Sequence Viewer

Length: 2712 bp
ATGCTCACCTCTATTCCCCCTCTTATCTTGTCTGGTGTTGTCATGAGCCTCACTATAAGGAAGTTGGCATCCCGCGGACAAGCTGCTTATTCAGTTGGAGCAACTGTGGTAGAGCAGACAATTGGTTCAATCAGAACTGTTGCGTCATTTACGGGGGAGAAGCAAGCTATAACTAAGTACAACAACTCCTTAGCTAAAGCTTACAAGTCTGGTGTGCAAGAGGGTTTAGCATCAGGAGTGGGTCTTGGTACGGTTATTCTTACTTTATTCTGCAGTTATGCATTGGCTATATGGTATGGCGGCAAAATGATACTTGAAAAGGGGTACAAAGCAGGAGATGTCATCAATGTGATTTTTGCTGTATTTACTGGTTCTTTCTCCCTCGGGCAGGCATCTCCATGCGTGAGTGCATTTTCTGCGGGACAAGCTGCAGCTTATAAGATGTTTGAGACAATCAACAGAAAGCCAGATATAGATGTGTATGACACCAGAGGACTAAAATCACAAGAGATCCGCGGAGACATTGAACTGAGGGATGTTTATTTTAGTTATCCTGCAAGACCGGATGAGCAAATATTCCTCGGATTTTCTCTCTCGATACCTAGTGGTGCAACTGTTGCTTTGGTTGGACAAAGCGGAAGTGGTAAATCAACTGTCATCAGTTTGATTGAGAGATTTTATGATCCCCAGGCTGGTGAAGTTCTTATTGATGGGAATAATCTAAAAGAGTTTCAACTGAAATGGATCAGACAGAAAATAGGCCTTGTAAGCCAGGAACCAGTTTTGTTTACTGGTAGCATTAAAGATAATATTGGCTATGGAAAGGATGATGCAACAACTGAAGAAATGAGGGCTGCTGCTGAGCTGGCCAATGCTGCAAAATTCATAGACAAACTACCTCAGGGACTAGATACAATGGTTGGTGAACATGGAACTCAGCTATCTGGGGGCCAAAAGCAGAGAATTGCCATAGCAAGAGCAATTCTGAAGAACCCAAGAATTCTGCTTTTGGATGAAGCAACAAGCGCTCTTGATGCAGAATCTGAGAGAGTTGTGCAAGAGGCATTGGACAGAATTATGGTTAACAGGACTACTGTCATTGTAGCCCATCGCTTGAGCACAGTGAGGACTGCTGATACAATTGCAGTAATACATCACGGAAAAATTGTTGAAAAAGGACCACATACTGAGCTGGTTAAGGATCCTGATGGTGCATATAGCCAACTTATAAGATTGCAAGAAATTAGCAGTGTGTCAGAACATGATGCTCAAAATGATCATGAAAGGCCAGAACTTTTCTCAAGTTTTCGGAAACTTTCTTCCTTTAGATCCATAAGTCCGGGGTCATCAGAAATAGGAAATAGTAGTCGCCGTAGTCACTCATTTTCAATCTCACATGGTGCTCCTACTGTAACCGGTGTCTTTGAAATAGCTCCTGCAGAACCTGACGTTCCAGCTGCATCGATAGAAGTGCATCCGGAAGTCTCACTTAGCCGCATGGCTTATCTGAACAAGACAGAAATCCCAGTACTACTTCTAGGTTCTATAGCTGCAGCAATCTGTGGCGTAATCTTGCCTATTTTTGGGGTGCTAATATCTAGCGTAATCAAGACCTTCTTTGATCCACCTCATCAGCTCCGAAAGGACTCAAAACTTTGGGCATTAATTTTTGTTGTTATTGGAGTGGTAGCTCTCTTGGCGCATCCAGTGAGAGCATACTTCTTTGCTGTGGCAGGGTGTAAGTTAATAAGACGAATCCGATCACTATGCTTCGAGAAGGTCATTTATATGGAAGCAAGTTGGTTTGATGAAGCCGAGCACTCGAGTGGTGCACTTGGTGCAAGGCTTTCAGGAAATGCAGCTTCTCTGCGAGGGCTGGTTGGAGATGCTCTCAGTTTGGCTGTTATGCATGCATCAACTACAATTGCTGGCTTGGTTATTGCTTTTTTGGCAAACTGGCAACTTGCTTTTATTATTCTTCTTATGCTGCCTCTACTAGGAATAAGTGGATATTTTCAAATCGAGGTCCTGAAAGGATTAAGCTCAAATGCAAAGAAAATGTATGAAGATGCAAGCCAAGTAGCCACCGATGCTGTGGGGAGTATTAGAACAATTGCTTCCTTTTGTGCTGAGGAAAAGATTATTAAGTTGTACCAGACAAAATGTGAAGGTCCCATCCAGGCAGGGATAAGACAAGGTATAGTTAGTGGAATAGGCTTTGGGCTATCACTCTTCTTGCTCTTTTCTGTATATGCATGCAGTTTCTATGCAGGGGCTCGACTTGTTGGAGCAGGCAAGACGACATTCCCAGATGTTTTCCGGGTTTTCTTTGCCCTCACTATGACAGCTGTGGCACTGTCTCAACAGGGTTCCTTAGCCCCTGATGCAAGCAAAGGAAAGAGCTCTGCTGCTTCAATATTTGCAATTCTTGACCGGATATCAAAAATAGATGCTAGTGATAACTCCGGAACAACTATAGAAAATTTGAAGGGAGAAATTGAATTTTGCCATGTTAGTTTCAAGTATCCAAATAGACCTAATGTACCAATCTTCCAGGATCTGTGCTTGACCATTCACTATGGGCAGACAGTTGCTCTGGTTGGAGAAAGCGGAAGTGGGAAATCAACAGTGATCTCATTGCTGCAGAGATTTTATGATCCTGATTCAGGTCACATTACATTAGATGGTACTGAAATGCAACATTACAGCTGA

Protein Analysis

903

Amino Acids

97.18

Weight (kDa)

7.27

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 2 - 126 2.3e-20 ABC transporter transmembrane region
ABC_tran PF00005 196 - 342 1.3e-34 ABC transporter
ABC_membrane PF00664 512 - 784 1.6e-53 ABC transporter transmembrane region
ABC_tran PF00005 852 - 901 1.8e-12 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02520 AT1G02520 AT1G02520 AT1G02530 AT1G02530 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT3G62150 AT3G62150 AT3G62150 AT4G01820 AT4G01820 AT4G01830 AT4G01830
fragaria_vesca FvH4_1g26280 FvH4_4g21370 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21381 FvH4_4g21600 FvH4_4g21600 FvH4_4g21600 FvH4_4g21620 FvH4_4g21621 FvH4_4g21622 FvH4_6g00641 FvH4_6g00650
malus_domestica MD09G1290100.v1.1 MD13G1138600.v1.1 MD13G1138700.v1.1 MD13G1139100.v1.1 MD16G1134500.v1.1 MD16G1134700.v1.1 MD16G1135000.v1.1 MD16G1135100.v1.1 MD17G1284300.v1.1 MD17G1284700.v1.1
prunus_persica Prupe.1G103800_v2.0.a1 Prupe.1G103800_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1
pyrus_communis pycom04g08750 pycom09g19770 pycom09g19790 pycom13g09140 pycom16g11900 pycom16g11920 pycom16g11930 pycom17g28030
rosa_chinensis RchiOBHm_Chr2g0157341 RchiOBHm_Chr3g0448061 RchiOBHm_Chr3g0485931 RchiOBHm_Chr3g0485961 RchiOBHm_Chr4g0427151 RchiOBHm_Chr4g0427161 RchiOBHm_Chr4g0427211 RchiOBHm_Chr4g0427241 RchiOBHm_Chr4g0427271 RchiOBHm_Chr4g0427711 RchiOBHm_Chr4g0427721 RchiOBHm_Chr4g0427771 RchiOBHm_Chr4g0427781 RchiOBHm_Chr4g0427791 RchiOBHm_Chr4g0427851 RchiOBHm_Chr4g0427861 RchiOBHm_Chr4g0446561 RchiOBHm_Chr5g0081601 RchiOBHm_Chr6g0278251 RchiOBHm_Chr6g0303241
rosa_laevigata RLG00000007206 RLG00000007207 RLG00000007209 RLG00000007210 RLG00000007211 RLG00000007212 RLG00000007214 RLG00000007255 RLG00000007256 RLG00000007257 RLG00000011097 RLG00000019257 RLG00000023121 RLG00000025948 RLG00000027246 RLG00000029989
rosa_multiflora Rmu_co8076196.1_g000001 Rmu_co8517461.1_g000001 Rmu_sc0000066.1_g000018 Rmu_sc0000066.1_g000024 Rmu_sc0000066.1_g000033 Rmu_sc0000066.1_g000047 Rmu_sc0000066.1_g000048 Rmu_sc0001651.1_g000024 Rmu_sc0002816.1_g000003 Rmu_sc0003337.1_g000015 Rmu_sc0003444.1_g000015 Rmu_sc0003813.1_g000004 Rmu_sc0004876.1_g000001 Rmu_sc0005969.1_g000025 Rmu_sc0008650.1_g000006 Rmu_sc0008855.1_g000001 Rmu_sc0009165.1_g000001 Rmu_sc0009165.1_g000002 Rmu_sc0010010.1_g000001 Rmu_sc0010010.1_g000005 Rmu_sc0036078.1_g000001
rosa_roxburghii Rroxscaffold_2G00130200 Rroxscaffold_5G00367590 Rroxscaffold_5G00368970 Rroxscaffold_5G00368990 Rroxscaffold_5G00369340 Rroxscaffold_5G00369350 Rroxscaffold_5G00369380 Rroxscaffold_5G00369390 Rroxscaffold_5G00369440 Rroxscaffold_5G00369450 Rroxscaffold_5G00369500 Rroxscaffold_5G00369510 Rroxscaffold_6G00396190 Rroxscaffold_6G00426220 Rroxscaffold_6G00427790 Rroxscaffold_7G00164940
rosa_rugosa Rorug01G0160800.1 Rorug02G0341400 Rorug02G0461800 Rorug02G0608000 Rorug03G0222700 Rorug03G0222800 Rorug03G0222900 Rorug04G0216400 Rorug04G0216400 Rorug04G0220100 Rorug04G0220100 Rorug04G0220100 Rorug05G0181800 Rorug05G0409700
rosa_samantha Rh1BG177300 Rh1DG206800 Rh2AG590600 Rh2BG473400 Rh2DG482300 Rh3AG007500 Rh3AG272400 Rh3BG007200 Rh3BG307000 Rh3DG301600 Rh3DG301900 Rh4AG272300 Rh4AG272400 Rh4AG272700 Rh4AG275900 Rh4AG276100 Rh4AG276200 Rh4AG276500 Rh4AG276600 Rh4AG276700 Rh4AG277000 Rh4BG281900 Rh4BG282200 Rh4CG292900 Rh4CG293300 Rh4CG293400 Rh4CG293600 Rh4CG297400 Rh4CG297500 Rh4CG297900 Rh4CG298000 Rh4CG298300 Rh4CG298400 Rh4DG165900 Rh5BG011400 Rh5DG092900 Rh6AG434000 Rh6CG153300 Rh6CG391700 Rh7BG333600
rosa_wichuraiana Rw0G005570 Rw0G005590 Rw0G011850 Rw0G015530 Rw0G017350 Rw0G019580 Rw3G000600 Rw3G024160 Rw4G023620 Rw4G023640 Rw4G023660 Rw4G023670 Rw4G023950 Rw4G023960 Rw4G024050 Rw4G024060 Rw5G048020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 438, 1229
AccII CGCG 2 cut(s) 75, 516
AccIII TCCGGA 2 cut(s) 1477, 2465
AciI CCGC 9 cut(s) 73, 75, 300, 419, 514, 516, 636, 1495, 2610
AclWI GGATC 9 cut(s) 505, 677, 752, 1196, 1209, 1323, 1616, 2565, 2651
AcoI YGGCCR 1 cut(s) 867
AcsI RAATTY 4 cut(s) 881, 999, 2482, 2501
AcuI CTGAAG 2 cut(s) 861, 1007
AdeI CACNNNGTG 2 cut(s) 1400, 1838
AfaI GTAC 7 cut(s) 179, 250, 326, 1530, 2153, 2544, 2689
AfeI AGCGCT 1 cut(s) 1027
AfiI CCNNNNNNNGG 5 cut(s) 388, 692, 1583, 1997, 2666
AgeI ACCGGT 1 cut(s) 1415
AjnI CCWGG 4 cut(s) 687, 771, 2178, 2553
AleI CACNNNNGTG 1 cut(s) 1824
Alw21I GWGCWC 5 cut(s) 1121, 1405, 1821, 1834, 2405
Alw26I GTCTC 4 cut(s) 443, 513, 1489, 2364
Alw44I GTGCAC 1 cut(s) 1830
AlwI GGATC 9 cut(s) 505, 677, 752, 1196, 1209, 1323, 1616, 2565, 2651
AlwNI CAGNNNCTG 3 cut(s) 1043, 1445, 2560
Ama87I CYCGRG 2 cut(s) 383, 1822
Aor13HI TCCGGA 2 cut(s) 1477, 2465
Aor51HI AGCGCT 1 cut(s) 1027
AoxI GGCC 4 cut(s) 760, 867, 949, 1286
ApaLI GTGCAC 1 cut(s) 1830
ApoI RAATTY 4 cut(s) 881, 999, 2482, 2501
AseI ATTAAT 1 cut(s) 1664
AsiGI ACCGGT 1 cut(s) 1415
AspLEI GCGC 2 cut(s) 1028, 1702
AspS9I GGNCC 4 cut(s) 949, 1178, 2026, 2171
AsuC2I CCSGG 2 cut(s) 1341, 2321
AsuHPI GGTGA 2 cut(s) 707, 935
AvaI CYCGRG 2 cut(s) 383, 1822
AvaII GGWCC 3 cut(s) 1178, 2026, 2171
AxyI CCTNAGG 1 cut(s) 900
BaeGI GKGCMC 1 cut(s) 1834
BaeI ACNNNNGTAYC 2 cut(s) 903, 936
BalI TGGCCA 1 cut(s) 869
BamHI GGATCC 1 cut(s) 1201
BanII GRGCYC 2 cut(s) 2278, 2405
Bbv12I GWGCWC 5 cut(s) 1121, 1405, 1821, 1834, 2405
BbvCI CCTCAGC 1 cut(s) 2130
BccI CCATC 5 cut(s) 704, 1116, 1202, 2183, 2678
BceAI ACGGC 1 cut(s) 1356
BcgI CGANNNNNNTGC 4 cut(s) 1453, 1487, 1749, 1783
BciT130I CCWGG 4 cut(s) 689, 773, 2180, 2555
BciVI GTATCC 1 cut(s) 2535
BclI TGATCA 1 cut(s) 1276
BcnI CCSGG 2 cut(s) 1341, 2321
BcoDI GTCTC 4 cut(s) 443, 513, 1489, 2364
BfaI CTAG 6 cut(s) 603, 908, 1538, 1599, 1997, 2454
BfmI CTRYAG 7 cut(s) 271, 429, 1437, 1545, 1551, 2475, 2642
BfoI RGCGCY 1 cut(s) 1029
BfuI GTATCC 1 cut(s) 2535
BlpI GCTNAGC 1 cut(s) 861
BmcAI AGTACT 1 cut(s) 1530
Bme1390I CCNGG 6 cut(s) 689, 773, 1341, 2180, 2321, 2555
Bme18I GGWCC 3 cut(s) 1178, 2026, 2171
BmeT110I CYCGRG 2 cut(s) 383, 1822
BmgT120I GGNCC 4 cut(s) 949, 1178, 2026, 2171
BmiI GGNNCC 5 cut(s) 777, 950, 1203, 2173, 2371
BmrFI CCNGG 6 cut(s) 689, 773, 1341, 2180, 2321, 2555
BmrI ACTGGG 1 cut(s) 1520
BmuI ACTGGG 1 cut(s) 1520
BoxI GACNNNNGTC 1 cut(s) 1094
BplI GAGNNNNNCTC 2 cut(s) 1875, 1907
Bpu10I CCTNAGC 3 cut(s) 190, 2130, 2374
Bpu1102I GCTNAGC 1 cut(s) 861
BpuEI CTTGAG 2 cut(s) 1135, 1285
BpuMI CCSGG 2 cut(s) 1341, 2321
Bsa29I ATCGAT 1 cut(s) 1463
BsaJI CCNNGG 6 cut(s) 73, 382, 514, 580, 687, 1340
BsaWI WCCGGW 5 cut(s) 562, 1415, 1477, 2433, 2465
BsaXI ACNNNNNCTCC 4 cut(s) 90, 120, 170, 200
Bsc4I CCNNNNNNNGG 5 cut(s) 388, 692, 1583, 1997, 2666
Bse118I RCCGGY 1 cut(s) 1415
Bse1I ACTGG 6 cut(s) 373, 779, 796, 1526, 1706, 1961
Bse21I CCTNAGG 1 cut(s) 900
Bse3DI GCAATG 1 cut(s) 2636
BseAI TCCGGA 2 cut(s) 1477, 2465
BseBI CCWGG 4 cut(s) 689, 773, 2180, 2555
BseCI ATCGAT 1 cut(s) 1463
BseDI CCNNGG 6 cut(s) 73, 382, 514, 580, 687, 1340
BseGI GGATG 8 cut(s) 68, 541, 571, 832, 1018, 1474, 1702, 2175
BseLI CCNNNNNNNGG 5 cut(s) 388, 692, 1583, 1997, 2666
BseMI GCAATG 1 cut(s) 2636
BseMII CTCAG 8 cut(s) 521, 852, 914, 950, 1035, 1179, 1906, 2121
BseNI ACTGG 6 cut(s) 373, 779, 796, 1526, 1706, 1961
BseSI GKGCMC 1 cut(s) 1834
Bsh1236I CGCG 2 cut(s) 75, 516
BshFI GGCC 4 cut(s) 762, 869, 951, 1288
BshTI ACCGGT 1 cut(s) 1415
BshVI ATCGAT 1 cut(s) 1463
BsiHKAI GWGCWC 5 cut(s) 1121, 1405, 1821, 1834, 2405
BsiHKCI CYCGRG 2 cut(s) 383, 1822
BsiSI CCGG 7 cut(s) 563, 1340, 1416, 1478, 2320, 2434, 2466
BslFI GGGAC 3 cut(s) 435, 918, 2157
BslI CCNNNNNNNGG 5 cut(s) 388, 692, 1583, 1997, 2666
BsmAI GTCTC 4 cut(s) 443, 513, 1489, 2364
BsmFI GGGAC 3 cut(s) 435, 918, 2157
BsnI GGCC 4 cut(s) 762, 869, 951, 1288
BsoBI CYCGRG 2 cut(s) 383, 1822
Bsp1286I GDGCHC 6 cut(s) 1121, 1405, 1821, 1834, 2278, 2405
Bsp13I TCCGGA 2 cut(s) 1477, 2465
Bsp1720I GCTNAGC 1 cut(s) 861
BspACI CCGC 9 cut(s) 73, 75, 300, 419, 514, 516, 636, 1495, 2610
BspANI GGCC 4 cut(s) 762, 869, 951, 1288
BspCNI CTCAG 8 cut(s) 522, 853, 913, 949, 1036, 1180, 1905, 2122
BspDI ATCGAT 1 cut(s) 1463
BspEI TCCGGA 2 cut(s) 1477, 2465
BspFNI CGCG 2 cut(s) 75, 516
BspHI TCATGA 2 cut(s) 42, 1279
BspLI GGNNCC 5 cut(s) 777, 950, 1203, 2173, 2371
BspMAI CTGCAG 5 cut(s) 275, 433, 1441, 1555, 2646
BspPI GGATC 9 cut(s) 505, 677, 752, 1196, 1209, 1323, 1616, 2565, 2651
BsrDI GCAATG 1 cut(s) 2636
BsrFI RCCGGY 1 cut(s) 1415
BsrI ACTGG 6 cut(s) 373, 779, 796, 1526, 1706, 1961
BssAI RCCGGY 1 cut(s) 1415
BssECI CCNNGG 6 cut(s) 73, 382, 514, 580, 687, 1340
Bst2UI CCWGG 4 cut(s) 689, 773, 2180, 2555
Bst6I CTCTTC 1 cut(s) 2237
BstAPI GCANNNNNTGC 3 cut(s) 416, 617, 1838
BstC8I GCNNGC 9 cut(s) 165, 390, 867, 1911, 1930, 2074, 2257, 2293, 2389
BstDSI CCRYGG 2 cut(s) 73, 514
BstENI CCTNNNNNAGG 3 cut(s) 386, 1995, 2664
BstF5I GGATG 8 cut(s) 68, 541, 571, 832, 1018, 1474, 1702, 2175
BstFNI CGCG 2 cut(s) 75, 516
BstH2I RGCGCY 1 cut(s) 1029
BstHHI GCGC 2 cut(s) 1028, 1702
BstMAI GTCTC 4 cut(s) 443, 513, 1489, 2364
BstNI CCWGG 4 cut(s) 689, 773, 2180, 2555
BstNSI RCATGY 2 cut(s) 1913, 2259
BstPAI GACNNNNGTC 1 cut(s) 1094
BstSCI CCNGG 6 cut(s) 687, 771, 1339, 2178, 2319, 2553
BstSFI CTRYAG 7 cut(s) 271, 429, 1437, 1545, 1551, 2475, 2642
BstSLI GKGCMC 1 cut(s) 1834
BstUI CGCG 2 cut(s) 75, 516
BstX2I RGATCY 4 cut(s) 510, 1201, 1328, 2557
BstYI RGATCY 4 cut(s) 510, 1201, 1328, 2557
Bsu15I ATCGAT 1 cut(s) 1463
Bsu36I CCTNAGG 1 cut(s) 900
BsuI GTATCC 1 cut(s) 2535
BsuRI GGCC 4 cut(s) 762, 869, 951, 1288
BsuTUI ATCGAT 1 cut(s) 1463
BtgI CCRYGG 2 cut(s) 73, 514
BtgZI GCGATG 1 cut(s) 1094
BtsCI GGATG 8 cut(s) 68, 541, 571, 832, 1018, 1474, 1702, 2175
BtsI GCAGTG 1 cut(s) 1255
BtsIMutI CAGTG 5 cut(s) 1128, 1255, 1713, 2354, 2634
Cac8I GCNNGC 9 cut(s) 165, 390, 867, 1911, 1930, 2074, 2257, 2293, 2389
CaiI CAGNNNCTG 3 cut(s) 1043, 1445, 2560
CciI TCATGA 2 cut(s) 42, 1279
CfoI GCGC 2 cut(s) 1028, 1702
Cfr10I RCCGGY 1 cut(s) 1415
Cfr13I GGNCC 4 cut(s) 949, 1178, 2026, 2171
Cfr42I CCGCGG 2 cut(s) 76, 517
ClaI ATCGAT 1 cut(s) 1463
CseI GACGC 1 cut(s) 132
Csp6I GTAC 7 cut(s) 178, 249, 325, 1529, 2152, 2543, 2688
CspAI ACCGGT 1 cut(s) 1415
CviQI GTAC 7 cut(s) 178, 249, 325, 1529, 2152, 2543, 2688
DraIII CACNNNGTG 2 cut(s) 1400, 1838
EaeI YGGCCR 1 cut(s) 867
Eam1104I CTCTTC 1 cut(s) 2237
EarI CTCTTC 1 cut(s) 2237
Ecl136II GAGCTC 1 cut(s) 2403
Eco147I AGGCCT 1 cut(s) 762
Eco24I GRGCYC 2 cut(s) 2278, 2405
Eco32I GATATC 1 cut(s) 2439
Eco47I GGWCC 3 cut(s) 1178, 2026, 2171
Eco47III AGCGCT 1 cut(s) 1027
Eco53kI GAGCTC 1 cut(s) 2403
Eco57I CTGAAG 2 cut(s) 861, 1007
Eco81I CCTNAGG 1 cut(s) 900
Eco88I CYCGRG 2 cut(s) 383, 1822
EcoICRI GAGCTC 1 cut(s) 2403
EcoNI CCTNNNNNAGG 3 cut(s) 386, 1995, 2664
EcoO109I RGGNCCY 2 cut(s) 2026, 2171
EcoRI GAATTC 1 cut(s) 999
EcoRII CCWGG 4 cut(s) 687, 771, 2178, 2553
EcoRV GATATC 1 cut(s) 2439
EcoT22I ATGCAT 4 cut(s) 283, 1911, 1915, 2257
EcoT38I GRGCYC 2 cut(s) 2278, 2405
FalI AAGNNNNNCTT 4 cut(s) 1473, 1505, 1601, 1633
FaqI GGGAC 3 cut(s) 435, 918, 2157
FauI CCCGC 2 cut(s) 80, 412
FbaI TGATCA 1 cut(s) 1276
FokI GGATG 8 cut(s) 55, 548, 578, 839, 1025, 1461, 1689, 2162
FriOI GRGCYC 2 cut(s) 2278, 2405
FspBI CTAG 6 cut(s) 603, 908, 1538, 1599, 1997, 2454
GlaI GCGC 2 cut(s) 1027, 1701
HaeII RGCGCY 1 cut(s) 1029
HaeIII GGCC 4 cut(s) 762, 869, 951, 1288
HapII CCGG 7 cut(s) 563, 1340, 1416, 1478, 2320, 2434, 2466
HgaI GACGC 1 cut(s) 132
HhaI GCGC 2 cut(s) 1028, 1702
Hin6I GCGC 2 cut(s) 1026, 1700
HinP1I GCGC 2 cut(s) 1026, 1700
HincII GTYRAC 1 cut(s) 1084
HindII GTYRAC 1 cut(s) 1084
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 4 cut(s) 1040, 1646, 1755, 2663
HpaI GTTAAC 1 cut(s) 1084
HpaII CCGG 7 cut(s) 563, 1340, 1416, 1478, 2320, 2434, 2466
HphI GGTGA 2 cut(s) 707, 935
Hpy166II GTNNAC 4 cut(s) 789, 926, 1084, 1832
Hpy8I GTNNAC 4 cut(s) 789, 926, 1084, 1832
HpyAV CCTTC 4 cut(s) 1624, 1771, 2162, 2482
HpyCH4IV ACGT 1 cut(s) 1449
HpySE526I ACGT 1 cut(s) 1449
HspAI GCGC 2 cut(s) 1026, 1700
Kpn2I TCCGGA 2 cut(s) 1477, 2465
Ksp22I TGATCA 1 cut(s) 1276
KspAI GTTAAC 1 cut(s) 1084
KspI CCGCGG 2 cut(s) 76, 517
LmnI GCTCC 5 cut(s) 98, 1408, 1438, 1641, 2288
MaeI CTAG 6 cut(s) 603, 908, 1538, 1599, 1997, 2454
MaeII ACGT 1 cut(s) 1449
MaeIII GTNAC 3 cut(s) 1376, 1411, 2669
MboII GAAGA 7 cut(s) 854, 1000, 1311, 1970, 2078, 2224, 2542
MfeI CAATTG 4 cut(s) 120, 1140, 1923, 2112
MflI RGATCY 4 cut(s) 510, 1201, 1328, 2557
MhlI GDGCHC 6 cut(s) 1121, 1405, 1821, 1834, 2278, 2405
MlsI TGGCCA 1 cut(s) 869
MluNI TGGCCA 1 cut(s) 869
MlyI GAGTC 1 cut(s) 1640
MmeI TCCRAC 5 cut(s) 76, 607, 1861, 2266, 2581
Mox20I TGGCCA 1 cut(s) 869
Mph1103I ATGCAT 4 cut(s) 283, 1911, 1915, 2257
MroI TCCGGA 2 cut(s) 1477, 2465
MscI TGGCCA 1 cut(s) 869
MseI TTAA 7 cut(s) 801, 1083, 1197, 1664, 1745, 2039, 2145
MslI CAYNNNNRTG 4 cut(s) 347, 397, 1787, 1824
Msp20I TGGCCA 1 cut(s) 869
MspA1I CMGCKG 5 cut(s) 75, 516, 1457, 2348, 2709
MspI CCGG 7 cut(s) 563, 1340, 1416, 1478, 2320, 2434, 2466
MspR9I CCNGG 6 cut(s) 689, 773, 1341, 2180, 2321, 2555
MunI CAATTG 4 cut(s) 120, 1140, 1923, 2112
MvaI CCWGG 4 cut(s) 689, 773, 2180, 2555
MvnI CGCG 2 cut(s) 75, 516
NciI CCSGG 2 cut(s) 1341, 2321
NlaIV GGNNCC 5 cut(s) 777, 950, 1203, 2173, 2371
NmeAIII GCCGAG 1 cut(s) 1840
NmuCI GTSAC 2 cut(s) 1376, 2669
NsiI ATGCAT 4 cut(s) 283, 1911, 1915, 2257
NspI RCATGY 2 cut(s) 1913, 2259
OliI CACNNNNGTG 1 cut(s) 1824
PaeI GCATGC 2 cut(s) 1913, 2259
PaeR7I CTCGAG 1 cut(s) 1822
PagI TCATGA 2 cut(s) 42, 1279
PceI AGGCCT 1 cut(s) 762
PfeI GAWTC 3 cut(s) 1040, 1755, 2663
PfoI TCCNGGA 1 cut(s) 2553
PinAI ACCGGT 1 cut(s) 1415
PleI GAGTC 1 cut(s) 1640
PpsI GAGTC 1 cut(s) 1640
PpuMI RGGWCCY 2 cut(s) 2026, 2171
PshAI GACNNNNGTC 1 cut(s) 1094
PshBI ATTAAT 1 cut(s) 1664
PsiI TTATAA 2 cut(s) 438, 1229
Psp124BI GAGCTC 1 cut(s) 2405
Psp5II RGGWCCY 2 cut(s) 2026, 2171
Psp6I CCWGG 4 cut(s) 687, 771, 2178, 2553
PspGI CCWGG 4 cut(s) 687, 771, 2178, 2553
PspN4I GGNNCC 5 cut(s) 777, 950, 1203, 2173, 2371
PspPI GGNCC 4 cut(s) 949, 1178, 2026, 2171
PspPPI RGGWCCY 2 cut(s) 2026, 2171
PspXI VCTCGAGB 1 cut(s) 1822
PstI CTGCAG 5 cut(s) 275, 433, 1441, 1555, 2646
PstNI CAGNNNCTG 3 cut(s) 1043, 1445, 2560
PsuI RGATCY 4 cut(s) 510, 1201, 1328, 2557
PvuII CAGCTG 3 cut(s) 1457, 2348, 2709
RsaI GTAC 7 cut(s) 179, 250, 326, 1530, 2153, 2544, 2689
RsaNI GTAC 7 cut(s) 178, 249, 325, 1529, 2152, 2543, 2688
RseI CAYNNNNRTG 4 cut(s) 347, 397, 1787, 1824
SacI GAGCTC 1 cut(s) 2405
SacII CCGCGG 2 cut(s) 76, 517
SaqAI TTAA 7 cut(s) 801, 1083, 1197, 1664, 1745, 2039, 2145
Sau96I GGNCC 4 cut(s) 949, 1178, 2026, 2171
ScaI AGTACT 1 cut(s) 1530
SchI GAGTC 1 cut(s) 1640
ScrFI CCNGG 6 cut(s) 689, 773, 1341, 2180, 2321, 2555
SduI GDGCHC 6 cut(s) 1121, 1405, 1821, 1834, 2278, 2405
SfcI CTRYAG 7 cut(s) 271, 429, 1437, 1545, 1551, 2475, 2642
Sfr274I CTCGAG 1 cut(s) 1822
Sfr303I CCGCGG 2 cut(s) 76, 517
SgrBI CCGCGG 2 cut(s) 76, 517
SinI GGWCC 3 cut(s) 1178, 2026, 2171
SlaI CTCGAG 1 cut(s) 1822
SmiMI CAYNNNNRTG 4 cut(s) 347, 397, 1787, 1824
SmlI CTYRAG 3 cut(s) 1114, 1300, 1822
SmoI CTYRAG 3 cut(s) 1114, 1300, 1822
SphI GCATGC 2 cut(s) 1913, 2259
SseBI AGGCCT 1 cut(s) 762
SsiI CCGC 9 cut(s) 73, 75, 300, 419, 514, 516, 636, 1495, 2610
SspI AATATT 3 cut(s) 576, 811, 2418
SspMI CTAG 6 cut(s) 603, 908, 1538, 1599, 1997, 2454
SstI GAGCTC 1 cut(s) 2405
StuI AGGCCT 1 cut(s) 762
StyD4I CCNGG 6 cut(s) 687, 771, 1339, 2178, 2319, 2553
TaiI ACGT 1 cut(s) 1452
TaqI TCGA 6 cut(s) 596, 1463, 1773, 1823, 2022, 2278
TatI WGTACW 2 cut(s) 177, 1528
TauI GCSGC 2 cut(s) 303, 1497
TfiI GAWTC 3 cut(s) 1040, 1755, 2663
Tru1I TTAA 7 cut(s) 801, 1083, 1197, 1664, 1745, 2039, 2145
Tru9I TTAA 7 cut(s) 801, 1083, 1197, 1664, 1745, 2039, 2145
TscAI CASTG 5 cut(s) 1128, 1255, 1713, 2361, 2634
TseFI GTSAC 2 cut(s) 1376, 2669
Tsp45I GTSAC 2 cut(s) 1376, 2669
TspDTI ATGAA 5 cut(s) 874, 1029, 1296, 1824, 2079
TspGWI ACGGA 1 cut(s) 1173
TspRI CASTG 5 cut(s) 1128, 1255, 1713, 2361, 2634
VneI GTGCAC 1 cut(s) 1830
VpaK11BI GGWCC 3 cut(s) 1178, 2026, 2171
VspI ATTAAT 1 cut(s) 1664
XagI CCTNNNNNAGG 3 cut(s) 386, 1995, 2664
XapI RAATTY 4 cut(s) 881, 999, 2482, 2501
XceI RCATGY 2 cut(s) 1913, 2259
XcmI CCANNNNNNNNNTGG 1 cut(s) 2092
XhoI CTCGAG 1 cut(s) 1822
XspI CTAG 6 cut(s) 603, 908, 1538, 1599, 1997, 2454
ZrmI AGTACT 1 cut(s) 1530
Zsp2I ATGCAT 4 cut(s) 283, 1911, 1915, 2257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.