ABC transporter B family member
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 1074, 1865, 2618 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1953 |
| Acc16I | TGCGCA | 1 cut(s) | 683 |
| Acc36I | ACCTGC | 1 cut(s) | 192 |
| AccB1I | GGYRCC | 3 cut(s) | 128, 671, 3613 |
| AccBSI | CCGCTC | 1 cut(s) | 3550 |
| AccI | GTMKAC | 1 cut(s) | 339 |
| AciI | CCGC | 7 cut(s) | 20, 347, 1747, 2004, 2196, 3550, 3755 |
| AclWI | GGATC | 6 cut(s) | 1388, 1971, 1999, 2161, 3210, 3714 |
| AcoI | YGGCCR | 1 cut(s) | 64 |
| AcsI | RAATTY | 3 cut(s) | 1517, 1635, 2554 |
| AcuI | CTGAAG | 4 cut(s) | 1497, 1863, 2085, 2796 |
| AfaI | GTAC | 6 cut(s) | 141, 202, 2188, 2465, 2708, 2798 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 23, 1328, 1986, 2642, 3032, 3310, 3311, 3397, 3700 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 3219 |
| AjiI | CACGTC | 1 cut(s) | 2087 |
| AjnI | CCWGG | 5 cut(s) | 1407, 2142, 2622, 3198, 3390 |
| Alw21I | GWGCWC | 5 cut(s) | 528, 1666, 1757, 3050, 3652 |
| Alw26I | GTCTC | 8 cut(s) | 400, 1020, 1079, 1149, 2134, 3009, 3283, 3361 |
| AlwI | GGATC | 6 cut(s) | 1388, 1971, 1999, 2161, 3210, 3714 |
| AlwNI | CAGNNNCTG | 5 cut(s) | 740, 1679, 2078, 2564, 3464 |
| AoxI | GGCC | 7 cut(s) | 64, 225, 537, 1396, 1585, 2116, 2815 |
| ApoI | RAATTY | 3 cut(s) | 1517, 1635, 2554 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 3223, 3255 |
| AseI | ATTAAT | 3 cut(s) | 267, 981, 1353 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 883, 1948, 2681 |
| AspLEI | GCGC | 1 cut(s) | 684 |
| AspS9I | GGNCC | 7 cut(s) | 225, 1585, 1814, 1837, 2117, 2816, 3691 |
| AsuC2I | CCSGG | 1 cut(s) | 2966 |
| AsuHPI | GGTGA | 5 cut(s) | 527, 619, 1571, 2797, 3397 |
| AsuII | TTCGAA | 2 cut(s) | 2294, 2752 |
| AvaII | GGWCC | 3 cut(s) | 1814, 1837, 3691 |
| AxyI | CCTNAGG | 1 cut(s) | 1536 |
| BaeGI | GKGCMC | 1 cut(s) | 2049 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 3525, 3558 |
| BanI | GGYRCC | 3 cut(s) | 128, 671, 3613 |
| BanII | GRGCYC | 3 cut(s) | 2871, 2923, 3050 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2414, 2446 |
| BauI | CACGAG | 2 cut(s) | 1657, 3597 |
| BbsI | GAAGAC | 1 cut(s) | 1951 |
| Bbv12I | GWGCWC | 5 cut(s) | 528, 1666, 1757, 3050, 3652 |
| BbvCI | CCTCAGC | 1 cut(s) | 6 |
| BceAI | ACGGC | 1 cut(s) | 79 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2394, 2428 |
| BciT130I | CCWGG | 5 cut(s) | 1409, 2144, 2624, 3200, 3392 |
| BciVI | GTATCC | 1 cut(s) | 3180 |
| BclI | TGATCA | 1 cut(s) | 1716 |
| BcnI | CCSGG | 1 cut(s) | 2966 |
| BcoDI | GTCTC | 8 cut(s) | 400, 1020, 1079, 1149, 2134, 3009, 3283, 3361 |
| BfuAI | ACCTGC | 1 cut(s) | 192 |
| BfuI | GTATCC | 1 cut(s) | 3180 |
| BlpI | GCTNAGC | 1 cut(s) | 1925 |
| BmcAI | AGTACT | 1 cut(s) | 2465 |
| Bme1390I | CCNGG | 6 cut(s) | 1409, 2144, 2624, 2966, 3200, 3392 |
| Bme18I | GGWCC | 3 cut(s) | 1814, 1837, 3691 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 3219 |
| BmgBI | CACGTC | 1 cut(s) | 2087 |
| BmgT120I | GGNCC | 7 cut(s) | 225, 1585, 1814, 1837, 2117, 2816, 3691 |
| BmrFI | CCNGG | 6 cut(s) | 1409, 2144, 2624, 2966, 3200, 3392 |
| BmrI | ACTGGG | 2 cut(s) | 498, 2165 |
| BmuI | ACTGGG | 2 cut(s) | 498, 2165 |
| BoxI | GACNNNNGTC | 2 cut(s) | 2718, 3275 |
| BpiI | GAAGAC | 1 cut(s) | 1951 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1605, 1637 |
| BpmI | CTGGAG | 3 cut(s) | 420, 2106, 2937 |
| Bpu10I | CCTNAGC | 1 cut(s) | 6 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1925 |
| Bpu14I | TTCGAA | 2 cut(s) | 2294, 2752 |
| BpuEI | CTTGAG | 4 cut(s) | 1060, 1771, 2452, 3341 |
| BpuMI | CCSGG | 1 cut(s) | 2966 |
| BsaAI | YACGTR | 1 cut(s) | 343 |
| BsaBI | GATNNNNATC | 1 cut(s) | 3821 |
| BsaJI | CCNNGG | 3 cut(s) | 318, 540, 1150 |
| BsaWI | WCCGGW | 5 cut(s) | 845, 1198, 1722, 3078, 3566 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 582, 612, 806, 836, 960, 990 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 23, 1328, 1986, 2642, 3032, 3310, 3311, 3397, 3700 |
| Bse118I | RCCGGY | 1 cut(s) | 3397 |
| Bse21I | CCTNAGG | 1 cut(s) | 1536 |
| Bse3DI | GCAATG | 4 cut(s) | 1776, 3429, 3786, 3854 |
| Bse8I | GATNNNNATC | 1 cut(s) | 3821 |
| BseBI | CCWGG | 5 cut(s) | 1409, 2144, 2624, 3200, 3392 |
| BseDI | CCNNGG | 3 cut(s) | 318, 540, 1150 |
| BseJI | GATNNNNATC | 1 cut(s) | 3821 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 23, 1328, 1986, 2642, 3032, 3310, 3311, 3397, 3700 |
| BseMI | GCAATG | 4 cut(s) | 1776, 3429, 3786, 3854 |
| BseMII | CTCAG | 8 cut(s) | 84, 1157, 1550, 1586, 1671, 1815, 2474, 3020 |
| BseRI | GAGGAG | 2 cut(s) | 56, 2265 |
| BseSI | GKGCMC | 1 cut(s) | 2049 |
| BsgI | GTGCAG | 2 cut(s) | 1712, 2709 |
| BshFI | GGCC | 7 cut(s) | 66, 227, 539, 1398, 1587, 2118, 2817 |
| BshNI | GGYRCC | 3 cut(s) | 128, 671, 3613 |
| BsiHKAI | GWGCWC | 5 cut(s) | 528, 1666, 1757, 3050, 3652 |
| BsiSI | CCGG | 7 cut(s) | 846, 1199, 1723, 2965, 3079, 3398, 3567 |
| BslFI | GGGAC | 4 cut(s) | 218, 1554, 2869, 3393 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 23, 1328, 1986, 2642, 3032, 3310, 3311, 3397, 3700 |
| BsmAI | GTCTC | 8 cut(s) | 400, 1020, 1079, 1149, 2134, 3009, 3283, 3361 |
| BsmBI | CGTCTC | 1 cut(s) | 3283 |
| BsmFI | GGGAC | 4 cut(s) | 218, 1554, 2869, 3393 |
| BsmI | GAATGC | 2 cut(s) | 1771, 3457 |
| BsnI | GGCC | 7 cut(s) | 66, 227, 539, 1398, 1587, 2118, 2817 |
| Bsp119I | TTCGAA | 2 cut(s) | 2294, 2752 |
| Bsp1286I | GDGCHC | 8 cut(s) | 528, 1666, 1757, 2049, 2871, 2923, 3050, 3652 |
| Bsp143I | GATC | 9 cut(s) | 1380, 1716, 1976, 1991, 2166, 2405, 3202, 3706, 3816 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1925 |
| Bsp19I | CCATGG | 1 cut(s) | 540 |
| BspACI | CCGC | 7 cut(s) | 20, 347, 1747, 2004, 2196, 3550, 3755 |
| BspANI | GGCC | 7 cut(s) | 66, 227, 539, 1398, 1587, 2118, 2817 |
| BspCNI | CTCAG | 8 cut(s) | 83, 1158, 1549, 1585, 1672, 1816, 2473, 3019 |
| BspMAI | CTGCAG | 6 cut(s) | 364, 1069, 2056, 2500, 2906, 3484 |
| BspMI | ACCTGC | 1 cut(s) | 192 |
| BspPI | GGATC | 6 cut(s) | 1388, 1971, 1999, 2161, 3210, 3714 |
| BspT104I | TTCGAA | 2 cut(s) | 2294, 2752 |
| BspT107I | GGYRCC | 3 cut(s) | 128, 671, 3613 |
| BsrBI | CCGCTC | 1 cut(s) | 3550 |
| BsrDI | GCAATG | 4 cut(s) | 1776, 3429, 3786, 3854 |
| BsrFI | RCCGGY | 1 cut(s) | 3397 |
| BssAI | RCCGGY | 1 cut(s) | 3397 |
| BssECI | CCNNGG | 3 cut(s) | 318, 540, 1150 |
| BssMI | GATC | 9 cut(s) | 1380, 1716, 1976, 1991, 2166, 2405, 3202, 3706, 3816 |
| BssSI | CACGAG | 2 cut(s) | 1657, 3597 |
| BssT1I | CCWWGG | 2 cut(s) | 318, 540 |
| Bst2BI | CACGAG | 2 cut(s) | 1657, 3597 |
| Bst2UI | CCWGG | 5 cut(s) | 1409, 2144, 2624, 3200, 3392 |
| Bst6I | CTCTTC | 1 cut(s) | 2772 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1253, 2483, 3860 |
| BstBAI | YACGTR | 1 cut(s) | 343 |
| BstBI | TTCGAA | 2 cut(s) | 2294, 2752 |
| BstC8I | GCNNGC | 9 cut(s) | 801, 1026, 1330, 1859, 2352, 2902, 2938, 3399, 3861 |
| BstDSI | CCRYGG | 2 cut(s) | 540, 1150 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2640 |
| BstHHI | GCGC | 1 cut(s) | 684 |
| BstKTI | GATC | 9 cut(s) | 1383, 1719, 1979, 1994, 2169, 2408, 3205, 3709, 3819 |
| BstMAI | GTCTC | 8 cut(s) | 400, 1020, 1079, 1149, 2134, 3009, 3283, 3361 |
| BstMBI | GATC | 9 cut(s) | 1380, 1716, 1976, 1991, 2166, 2405, 3202, 3706, 3816 |
| BstNI | CCWGG | 5 cut(s) | 1409, 2144, 2624, 3200, 3392 |
| BstNSI | RCATGY | 1 cut(s) | 3863 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 2718, 3275 |
| BstSCI | CCNGG | 6 cut(s) | 1407, 2142, 2622, 2964, 3198, 3390 |
| BstSLI | GKGCMC | 1 cut(s) | 2049 |
| BstV2I | GAAGAC | 1 cut(s) | 1951 |
| BstX2I | RGATCY | 2 cut(s) | 2166, 3202 |
| BstYI | RGATCY | 2 cut(s) | 2166, 3202 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1536 |
| BsuI | GTATCC | 1 cut(s) | 3180 |
| BsuRI | GGCC | 7 cut(s) | 66, 227, 539, 1398, 1587, 2118, 2817 |
| BtgI | CCRYGG | 2 cut(s) | 540, 1150 |
| BtgZI | GCGATG | 1 cut(s) | 2999 |
| BtrI | CACGTC | 1 cut(s) | 2087 |
| BtsI | GCAGTG | 5 cut(s) | 81, 2049, 2345, 2745, 3774 |
| BveI | ACCTGC | 1 cut(s) | 192 |
| Cac8I | GCNNGC | 9 cut(s) | 801, 1026, 1330, 1859, 2352, 2902, 2938, 3399, 3861 |
| CaiI | CAGNNNCTG | 5 cut(s) | 740, 1679, 2078, 2564, 3464 |
| CfoI | GCGC | 1 cut(s) | 684 |
| Cfr10I | RCCGGY | 1 cut(s) | 3397 |
| Cfr13I | GGNCC | 7 cut(s) | 225, 1585, 1814, 1837, 2117, 2816, 3691 |
| CseI | GACGC | 2 cut(s) | 317, 2723 |
| Csp6I | GTAC | 6 cut(s) | 140, 201, 2187, 2464, 2707, 2797 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 3702, 3737 |
| CviQI | GTAC | 6 cut(s) | 140, 201, 2187, 2464, 2707, 2797 |
| DpnI | GATC | 9 cut(s) | 1382, 1718, 1978, 1993, 2168, 2407, 3204, 3708, 3818 |
| DpnII | GATC | 9 cut(s) | 1380, 1716, 1976, 1991, 2166, 2405, 3202, 3706, 3816 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1953 |
| DriI | GACNNNNNGTC | 1 cut(s) | 3219 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1953 |
| EaeI | YGGCCR | 1 cut(s) | 64 |
| Eam1104I | CTCTTC | 1 cut(s) | 2772 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 3219 |
| EarI | CTCTTC | 1 cut(s) | 2772 |
| Ecl136II | GAGCTC | 1 cut(s) | 3048 |
| Eco130I | CCWWGG | 2 cut(s) | 318, 540 |
| Eco147I | AGGCCT | 1 cut(s) | 1398 |
| Eco24I | GRGCYC | 3 cut(s) | 2871, 2923, 3050 |
| Eco32I | GATATC | 2 cut(s) | 1147, 2241 |
| Eco47I | GGWCC | 3 cut(s) | 1814, 1837, 3691 |
| Eco53kI | GAGCTC | 1 cut(s) | 3048 |
| Eco57I | CTGAAG | 4 cut(s) | 1497, 1863, 2085, 2796 |
| Eco81I | CCTNAGG | 1 cut(s) | 1536 |
| EcoICRI | GAGCTC | 1 cut(s) | 3048 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2640 |
| EcoO109I | RGGNCCY | 4 cut(s) | 225, 1837, 2117, 2816 |
| EcoRI | GAATTC | 2 cut(s) | 1635, 2554 |
| EcoRII | CCWGG | 5 cut(s) | 1407, 2142, 2622, 3198, 3390 |
| EcoRV | GATATC | 2 cut(s) | 1147, 2241 |
| EcoT14I | CCWWGG | 2 cut(s) | 318, 540 |
| EcoT22I | ATGCAT | 1 cut(s) | 3688 |
| EcoT38I | GRGCYC | 3 cut(s) | 2871, 2923, 3050 |
| ErhI | CCWWGG | 2 cut(s) | 318, 540 |
| Esp3I | CGTCTC | 1 cut(s) | 3283 |
| FaqI | GGGAC | 4 cut(s) | 218, 1554, 2869, 3393 |
| FauI | CCCGC | 1 cut(s) | 3543 |
| FauNDI | CATATG | 2 cut(s) | 2038, 3436 |
| FbaI | TGATCA | 1 cut(s) | 1716 |
| FblI | GTMKAC | 1 cut(s) | 339 |
| FriOI | GRGCYC | 3 cut(s) | 2871, 2923, 3050 |
| FspAI | RTGCGCAY | 1 cut(s) | 683 |
| FspI | TGCGCA | 1 cut(s) | 683 |
| GlaI | GCGC | 1 cut(s) | 683 |
| GsuI | CTGGAG | 3 cut(s) | 420, 2106, 2937 |
| HaeIII | GGCC | 7 cut(s) | 66, 227, 539, 1398, 1587, 2118, 2817 |
| HapII | CCGG | 7 cut(s) | 846, 1199, 1723, 2965, 3079, 3398, 3567 |
| HgaI | GACGC | 2 cut(s) | 317, 2723 |
| HhaI | GCGC | 1 cut(s) | 684 |
| Hin6I | GCGC | 1 cut(s) | 682 |
| HinP1I | GCGC | 1 cut(s) | 682 |
| HincII | GTYRAC | 2 cut(s) | 307, 1002 |
| HindII | GTYRAC | 2 cut(s) | 307, 1002 |
| HindIII | AAGCTT | 2 cut(s) | 152, 834 |
| HpaII | CCGG | 7 cut(s) | 846, 1199, 1723, 2965, 3079, 3398, 3567 |
| HphI | GGTGA | 5 cut(s) | 527, 619, 1571, 2797, 3397 |
| Hpy166II | GTNNAC | 9 cut(s) | 307, 340, 713, 786, 1002, 1817, 2431, 3518, 3572 |
| Hpy8I | GTNNAC | 9 cut(s) | 307, 340, 713, 786, 1002, 1817, 2431, 3518, 3572 |
| Hpy99I | CGWCG | 1 cut(s) | 2142 |
| HpyCH4IV | ACGT | 3 cut(s) | 342, 2086, 3186 |
| HpySE526I | ACGT | 3 cut(s) | 342, 2086, 3186 |
| HspAI | GCGC | 1 cut(s) | 682 |
| KroI | GCCGGC | 1 cut(s) | 3397 |
| KroNI | GCCGGC | 1 cut(s) | 3399 |
| Ksp22I | TGATCA | 1 cut(s) | 1716 |
| Kzo9I | GATC | 9 cut(s) | 1380, 1716, 1976, 1991, 2166, 2405, 3202, 3706, 3816 |
| LmnI | GCTCC | 6 cut(s) | 114, 353, 1013, 1847, 2918, 3394 |
| MaeII | ACGT | 3 cut(s) | 342, 2086, 3186 |
| MaeIII | GTNAC | 2 cut(s) | 394, 3314 |
| MalI | GATC | 9 cut(s) | 1382, 1718, 1978, 1993, 2168, 2407, 3204, 3708, 3818 |
| MbiI | CCGCTC | 1 cut(s) | 3550 |
| MboI | GATC | 9 cut(s) | 1380, 1716, 1976, 1991, 2166, 2405, 3202, 3706, 3816 |
| MboII | GAAGA | 5 cut(s) | 636, 1490, 1956, 2789, 2815 |
| MfeI | CAATTG | 8 cut(s) | 756, 1089, 1800, 2478, 2568, 2757, 3560, 3591 |
| MflI | RGATCY | 2 cut(s) | 2166, 3202 |
| MhlI | GDGCHC | 8 cut(s) | 528, 1666, 1757, 2049, 2871, 2923, 3050, 3652 |
| MlyI | GAGTC | 3 cut(s) | 77, 3089, 3792 |
| MmeI | TCCRAC | 4 cut(s) | 1243, 2506, 3197, 3226 |
| Mph1103I | ATGCAT | 1 cut(s) | 3688 |
| MroNI | GCCGGC | 1 cut(s) | 3397 |
| MroXI | GAANNNNTTC | 3 cut(s) | 883, 1948, 2681 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1033, 3864 |
| MspA1I | CMGCKG | 2 cut(s) | 365, 995 |
| MspI | CCGG | 7 cut(s) | 846, 1199, 1723, 2965, 3079, 3398, 3567 |
| MspR9I | CCNGG | 6 cut(s) | 1409, 2144, 2624, 2966, 3200, 3392 |
| MunI | CAATTG | 8 cut(s) | 756, 1089, 1800, 2478, 2568, 2757, 3560, 3591 |
| Mva1269I | GAATGC | 2 cut(s) | 1771, 3457 |
| MvaI | CCWGG | 5 cut(s) | 1409, 2144, 2624, 3200, 3392 |
| NaeI | GCCGGC | 1 cut(s) | 3399 |
| NciI | CCSGG | 1 cut(s) | 2966 |
| NcoI | CCATGG | 1 cut(s) | 540 |
| NdeI | CATATG | 2 cut(s) | 2038, 3436 |
| NdeII | GATC | 9 cut(s) | 1380, 1716, 1976, 1991, 2166, 2405, 3202, 3706, 3816 |
| NgoMIV | GCCGGC | 1 cut(s) | 3397 |
| NmuCI | GTSAC | 2 cut(s) | 394, 3314 |
| NsbI | TGCGCA | 1 cut(s) | 683 |
| NsiI | ATGCAT | 1 cut(s) | 3688 |
| NspI | RCATGY | 1 cut(s) | 3863 |
| NspV | TTCGAA | 2 cut(s) | 2294, 2752 |
| PacI | TTAATTAA | 1 cut(s) | 831 |
| PaeI | GCATGC | 1 cut(s) | 3863 |
| PceI | AGGCCT | 1 cut(s) | 1398 |
| PctI | GAATGC | 2 cut(s) | 1771, 3457 |
| PdiI | GCCGGC | 1 cut(s) | 3399 |
| PdmI | GAANNNNTTC | 3 cut(s) | 883, 1948, 2681 |
| PfoI | TCCNGGA | 1 cut(s) | 3198 |
| PleI | GAGTC | 3 cut(s) | 77, 3089, 3791 |
| PpsI | GAGTC | 3 cut(s) | 77, 3089, 3791 |
| Ppu21I | YACGTR | 1 cut(s) | 343 |
| PpuMI | RGGWCCY | 1 cut(s) | 1837 |
| PshAI | GACNNNNGTC | 2 cut(s) | 2718, 3275 |
| PshBI | ATTAAT | 3 cut(s) | 267, 981, 1353 |
| PsiI | TTATAA | 3 cut(s) | 1074, 1865, 2618 |
| Psp124BI | GAGCTC | 1 cut(s) | 3050 |
| Psp5II | RGGWCCY | 1 cut(s) | 1837 |
| Psp6I | CCWGG | 5 cut(s) | 1407, 2142, 2622, 3198, 3390 |
| PspGI | CCWGG | 5 cut(s) | 1407, 2142, 2622, 3198, 3390 |
| PspPI | GGNCC | 7 cut(s) | 225, 1585, 1814, 1837, 2117, 2816, 3691 |
| PspPPI | RGGWCCY | 1 cut(s) | 1837 |
| PstI | CTGCAG | 6 cut(s) | 364, 1069, 2056, 2500, 2906, 3484 |
| PstNI | CAGNNNCTG | 5 cut(s) | 740, 1679, 2078, 2564, 3464 |
| PsuI | RGATCY | 2 cut(s) | 2166, 3202 |
| PvuII | CAGCTG | 2 cut(s) | 365, 995 |
| RsaI | GTAC | 6 cut(s) | 141, 202, 2188, 2465, 2708, 2798 |
| RsaNI | GTAC | 6 cut(s) | 140, 201, 2187, 2464, 2707, 2797 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1033, 3864 |
| SacI | GAGCTC | 1 cut(s) | 3050 |
| Sau3AI | GATC | 9 cut(s) | 1380, 1716, 1976, 1991, 2166, 2405, 3202, 3706, 3816 |
| Sau96I | GGNCC | 7 cut(s) | 225, 1585, 1814, 1837, 2117, 2816, 3691 |
| ScaI | AGTACT | 1 cut(s) | 2465 |
| SchI | GAGTC | 3 cut(s) | 77, 3089, 3792 |
| ScrFI | CCNGG | 6 cut(s) | 1409, 2144, 2624, 2966, 3200, 3392 |
| SduI | GDGCHC | 8 cut(s) | 528, 1666, 1757, 2049, 2871, 2923, 3050, 3652 |
| SfuI | TTCGAA | 2 cut(s) | 2294, 2752 |
| SinI | GGWCC | 3 cut(s) | 1814, 1837, 3691 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1033, 3864 |
| SmlI | CTYRAG | 4 cut(s) | 1039, 1750, 2467, 3356 |
| SmoI | CTYRAG | 4 cut(s) | 1039, 1750, 2467, 3356 |
| SphI | GCATGC | 1 cut(s) | 3863 |
| SseBI | AGGCCT | 1 cut(s) | 1398 |
| SsiI | CCGC | 7 cut(s) | 20, 347, 1747, 2004, 2196, 3550, 3755 |
| SspI | AATATT | 3 cut(s) | 447, 1212, 1447 |
| SstI | GAGCTC | 1 cut(s) | 3050 |
| StuI | AGGCCT | 1 cut(s) | 1398 |
| StyD4I | CCNGG | 6 cut(s) | 1407, 2142, 2622, 2964, 3198, 3390 |
| StyI | CCWWGG | 2 cut(s) | 318, 540 |
| TaiI | ACGT | 3 cut(s) | 345, 2089, 3189 |
| TaqII | GACCGA | 1 cut(s) | 3708 |
| TatI | WGTACW | 1 cut(s) | 2463 |
| TauI | GCSGC | 1 cut(s) | 2198 |
| TseFI | GTSAC | 2 cut(s) | 394, 3314 |
| Tsp45I | GTSAC | 2 cut(s) | 394, 3314 |
| TspGWI | ACGGA | 2 cut(s) | 1139, 2273 |
| VpaK11BI | GGWCC | 3 cut(s) | 1814, 1837, 3691 |
| VspI | ATTAAT | 3 cut(s) | 267, 981, 1353 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2640 |
| XapI | RAATTY | 3 cut(s) | 1517, 1635, 2554 |
| XceI | RCATGY | 1 cut(s) | 3863 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2737 |
| XmiI | GTMKAC | 1 cut(s) | 339 |
| XmnI | GAANNNNTTC | 3 cut(s) | 883, 1948, 2681 |
| ZrmI | AGTACT | 1 cut(s) | 2465 |
| Zsp2I | ATGCAT | 1 cut(s) | 3688 |