Rroxscaffold_6G00396190

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000116
Physical Location & Seq
Forward (+)
17512983 .. 17519293
6311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00396190.1

Sequence Viewer

Length: 3837 bp
ATGGCTGAGGAGAATGGTGTGAATGGTGATACTAAAAATCATGAAAACCATGCAGAAGAAGAGAAAAGCTCAGCCCCCATGAATAAGGATCAGCCAGGCTCATCATCTAGCAATGGAGATGAGAAAGTTGAAAAGATTCCTTTCTCCAAGCTATTCTCCTTTGCAGATAAGACTGATGTTATCTTGATGGTTGTCGGCACGATTGGTGCCATTGGGAATGGTTCTTGTATGCCCCTCATGACGGTATTGTTTGGGGAGATGATAGATTCTTTTGGATCTAATCAAAATTCAGACATAGTCTCAGTTGTTTCAAAGGTCTCTCTGAAATTTGTTTACCTGGCTGTGGGAGCAGCTGTGGCAGCATTTCTCCAGGTGTCTTGCTGGATGGTCACAGGGGAAAGACAAGCTGCAAGAATTAGAGGTTTGTATTTGAAAACAATATTGAGACAAGATGTTGCTTTCTTCGATATGGAAACAAACACTGGAGAGGTAGTTGGGAGAATGTCTGGTGACACTGTTCTCATTCAAGATGCCATGGGGGAGAAGGTTGGAAAATTCTTACAGCTGCTTTCAACATTCATTGGAGGGTTTATAGTAGCATTCATCAAAGGGTGGCTTCTCACTCTTGTCATGCTATCCTCTATTCCTTTGCTTGTGGCATCTGGTGCAACGATGTCCATCATTATAACCAAGATGGCATCCCGTGGACAAACTGCTTACGCAAAAGCAGCAAATGTAGTTGAACAAACAATTGGGTCCATTAGAACCGTTGCATCATTTACTGGCGAGAGGGAAGCTATAAATAGTTATAACAAATATCTCGTAGATGCTTACAAATCAGGTGTTCACGAAGGTTCAGCTGCTGGAATAGGTCTTGGCTTGGTTATGTGTGTTGTGTTCAGCTCATATGCCTTGGCTGTATGGTTTGGTTCAAAGATGATAAGGGAAAATGGATATACTGGGGGTGAAGTACTGAATGTGATTGTTGCTGTGTTGACTGGATCTATGTCTTTAGGGCAGACATCACCATGCATGAGTGCATTTGCTGCTGGTCAAGCTGCAGCTTATAAGATGTTTCAGACTATTAGCAGGAAGCCAGAGATAGATGCTTATGATGAAAAGGGAAAGACTTTGGGTGACATTTCGGGAGACATAGAGTTGAGAGATGTTTATTTCAGCTATCCAGCCAGACCGGAGGAGCAAATATTTGACGGGTTCTCTCTTTGTATCCCTAGTGGCACAACTGCTGCTTTGGTTGGACAAAGTGGAAGTGGCAAGTCAACAGTCATCAGTCTCATAGAGAGATTTTATGATCCGCAAGCTGGTGAAGTTTTAATAGATGGCATAAACCTAAAGGAATTTCAACTTAAATGGATTCGGAGTAAAATTGGTCTTGTCAGTCAGGAACCAGTCCTGTTTGCATCCACCATTAAGGAAAATATTGCCTATGGAAAGGATGGTGCTACCACTGAAGAGATACAAGCAGCAGCCGAGCTTGCAAATGCGGCTAAATTTGTTGATAAATTGCCTCAGGGCCTTGACAGCTTGGTTGGTGAGCATGGAACTCAGTTGTCTGGTGGGCAGAAGCAGAGAATTGCTATTGCAAGAGCAATTTTGAAAGACCCAAGGATTTTACTTCTGGATGAAGCTACAAGTGCACTTGATGCAGAATCTGAGAGGGTAGTTCAAGAAGCATTGGATAGGATTATGGTTAACCGAACTACTGTTGTTGTCGCTCATCGTTTGAGTACAGTGAGGAATGCAGACATGATTGCTGTCATTCATAAAGGAAAGATGGTTGAAAAAGGTTCGCACTTGAATTTACTCAAAGATCCTGAGGGAGCTTACTCTCAGCTTATACGCTTGCAAGAAGTAAACAAAGAGTCAGAACAAACGTCAGAAGACCAAAACAAACCTGAAATAACTCTGGCATCTCTTAGACAGTCGAGTCAAAAAACATCGAGTCAAAGACTTTCGTTTGCACGATCCTTAAGTAGAAATTCTTCTGTAGGAAATAGCAGCCGTCACTCATATTCAATGGCATTTGGTTTACCCACTGGACTTGGAGGCATTGGTGTCCAAGATGCTGCATATGACGAAACAGAGCTAGCACCAGAGCAACCTCCAAAGGTCTCACTCCGCCGCGTTGCTGCCCTGAACAAACCTGAGATTCCAGTTCTTATTGTTGGAACTATAGCTGCAATCATCAATGGTGTTATACTTCCAATATTCGGTGTGCTCATTTCCAGGGTTATAAAGACTTTCTATGAACCACCTCATCAACAGAAGAAAGATGCAGCATTTTGGGCAATAATTTTTGTGATCCTTGGTCTGATATCATTCGTGGTGATCCCAGCACGAGGATATTTCTTTTCAGTAGCTGGCAGCAAGTTAATTCAGCGTATCAGATTGTTGTGTTTTGAGAGAGTGGTTCACATGGAGGTTGGGTGGTTTGATGAGCCTGAGAACTCAAGTGGTGCAATTGGTGCAAGGCTCTCAGCTGATGCAGCGTCGGTGAGGGCCCTAGTCGGAGACGCTCTAGCTCAGATGGTTCAAAACATAGCAGCTGCAGTTGCAGGTTTGGTCATTGCTTATATCGCATGTTGGCAGCTGGCATTTATTATCCTCGCACTGCTTCCTCTCATTGCAGTTAACGGATATGTACAAATAAAGTTCATGAAGGGATTCAGCGCAGATGCAAAGATGATGTATGAGGAGGCAAGCCAAGTTGCTAATGACGCAGTTGGAAGTATAAGAACAGTTGCTTCTTTCTGTGCTGAAGAGAAGGTAATGGAACTATACAGAAGGAAATGTGAAGGCCCTATGAAGACAGGGATAAGACAAGGCTTGATCAGTGGTATAGGATTTGGGGTATCTTTCTTCTTTTTGTTTTGTGTCTACGCAACCAGTTTCTATGCCGGAGCTCAACTCGTGAAGGCTGGCAAAACAACATTTACAGACGTTTTCCAAGTTTTCTTTGCTCTGACCATGGCAGCTACTGGAATTTCTCAATCAAGCTCCTTTGGTCCCGATTCTGGTAAAGCCAAAACTTCTGCTGCTTCCATATTTGCAATAATAGACCGGCCATCAAAGATAGACCCAAGTAACGAGTCTGGTACGAAAATTGATGGTGGTGTGAAAGGAGAGATTGAGCTTCGCCATGTAAGCTTTAAGTATCCATCTAGGCCAGAAACACCTATTTTCCGAGACCTCAATTTAACTATCCGCTCTGGCAAGACGGTTGCCCTGGTCGGAGAAAGTGGGAGCGGAAAATCAACTGTTGTTGCATTGTTGCAAAGATTTTATGATCCGGATTCAGGTCATATCACACTTGATGGAATTGAACTTGGCAACTACAACTTGAAATGGTTGAGGCAGCAGATGGGACTCGTAAGCCAAGAACCTGTTTTATTTAATGACACCATTCGCGCCAACATTGCATATGGAAAGGAAGAAACTGCAACTGAGGCAGAAATTATAGCTGCATCAGAGTTGGCCAATGCCCACAAGTTCATCAGTAGCCTACACCAGAAGCAACGTGTAGCCATTGCGCGTGCTATAATCAAGAGCCCAAAGATATTACTGTTGGATGAAGCTACGAGTGCACTTGATGCCGAATCTGAGAGAGTGGTTCAAGATGCATTAGACCGAGTCATGGTGAACCGGACAACAGTGGTGGTGGCTCATAGACTATCCACAATCAAGAATGCTGATGTGATTGCAGTGGTTAAAAATGGAGTTATAGTAGAGAAAGGGAAGCATGACAATTTGATTAATATCAGCGATGGGTTTTATGCATCCCTAGTTGCTCTTCACATGAGCTCTTCAACTGCATGA

Protein Analysis

1278

Amino Acids

137.69

Weight (kDa)

6.55

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 64 - 336 1e-59 ABC transporter transmembrane region
ABC_tran PF00005 404 - 552 2.2e-33 ABC transporter
ABC_membrane PF00664 727 - 998 5.4e-59 ABC transporter transmembrane region
ABC_tran PF00005 1069 - 1201 1.8e-28 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02520 AT1G02520 AT1G02520 AT1G02530 AT1G02530 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT3G62150 AT3G62150 AT3G62150 AT4G01820 AT4G01820 AT4G01830 AT4G01830
fragaria_vesca FvH4_1g26280 FvH4_4g21370 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21381 FvH4_4g21600 FvH4_4g21600 FvH4_4g21600 FvH4_4g21620 FvH4_4g21621 FvH4_4g21622 FvH4_6g00641 FvH4_6g00650
malus_domestica MD09G1290100.v1.1 MD13G1138600.v1.1 MD13G1138700.v1.1 MD13G1139100.v1.1 MD16G1134500.v1.1 MD16G1134700.v1.1 MD16G1135000.v1.1 MD16G1135100.v1.1 MD17G1284300.v1.1 MD17G1284700.v1.1
prunus_persica Prupe.1G103800_v2.0.a1 Prupe.1G103800_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1
pyrus_communis pycom04g08750 pycom09g19770 pycom09g19790 pycom13g09140 pycom16g11900 pycom16g11920 pycom16g11930 pycom17g28030
rosa_chinensis RchiOBHm_Chr2g0157341 RchiOBHm_Chr3g0448061 RchiOBHm_Chr3g0485931 RchiOBHm_Chr3g0485961 RchiOBHm_Chr4g0427151 RchiOBHm_Chr4g0427161 RchiOBHm_Chr4g0427211 RchiOBHm_Chr4g0427241 RchiOBHm_Chr4g0427271 RchiOBHm_Chr4g0427711 RchiOBHm_Chr4g0427721 RchiOBHm_Chr4g0427771 RchiOBHm_Chr4g0427781 RchiOBHm_Chr4g0427791 RchiOBHm_Chr4g0427851 RchiOBHm_Chr4g0427861 RchiOBHm_Chr4g0446561 RchiOBHm_Chr5g0081601 RchiOBHm_Chr6g0278251 RchiOBHm_Chr6g0303241
rosa_laevigata RLG00000007206 RLG00000007207 RLG00000007209 RLG00000007210 RLG00000007211 RLG00000007212 RLG00000007214 RLG00000007255 RLG00000007256 RLG00000007257 RLG00000011097 RLG00000019257 RLG00000023121 RLG00000025948 RLG00000027246 RLG00000029989
rosa_multiflora Rmu_co8076196.1_g000001 Rmu_co8517461.1_g000001 Rmu_sc0000066.1_g000018 Rmu_sc0000066.1_g000024 Rmu_sc0000066.1_g000033 Rmu_sc0000066.1_g000047 Rmu_sc0000066.1_g000048 Rmu_sc0001651.1_g000024 Rmu_sc0002816.1_g000003 Rmu_sc0003337.1_g000015 Rmu_sc0003444.1_g000015 Rmu_sc0003813.1_g000004 Rmu_sc0004876.1_g000001 Rmu_sc0005969.1_g000025 Rmu_sc0008650.1_g000006 Rmu_sc0008855.1_g000001 Rmu_sc0009165.1_g000001 Rmu_sc0009165.1_g000002 Rmu_sc0010010.1_g000001 Rmu_sc0010010.1_g000005 Rmu_sc0036078.1_g000001
rosa_roxburghii Rroxscaffold_2G00130200 Rroxscaffold_5G00367590 Rroxscaffold_5G00368970 Rroxscaffold_5G00368990 Rroxscaffold_5G00369340 Rroxscaffold_5G00369350 Rroxscaffold_5G00369380 Rroxscaffold_5G00369390 Rroxscaffold_5G00369440 Rroxscaffold_5G00369450 Rroxscaffold_5G00369500 Rroxscaffold_5G00369510 Rroxscaffold_6G00396190 Rroxscaffold_6G00426220 Rroxscaffold_6G00427790 Rroxscaffold_7G00164940
rosa_rugosa Rorug01G0160800.1 Rorug02G0341400 Rorug02G0461800 Rorug02G0608000 Rorug03G0222700 Rorug03G0222800 Rorug03G0222900 Rorug04G0216400 Rorug04G0216400 Rorug04G0220100 Rorug04G0220100 Rorug04G0220100 Rorug05G0181800 Rorug05G0409700
rosa_samantha Rh1BG177300 Rh1DG206800 Rh2AG590600 Rh2BG473400 Rh2DG482300 Rh3AG007500 Rh3AG272400 Rh3BG007200 Rh3BG307000 Rh3DG301600 Rh3DG301900 Rh4AG272300 Rh4AG272400 Rh4AG272700 Rh4AG275900 Rh4AG276100 Rh4AG276200 Rh4AG276500 Rh4AG276600 Rh4AG276700 Rh4AG277000 Rh4BG281900 Rh4BG282200 Rh4CG292900 Rh4CG293300 Rh4CG293400 Rh4CG293600 Rh4CG297400 Rh4CG297500 Rh4CG297900 Rh4CG298000 Rh4CG298300 Rh4CG298400 Rh4DG165900 Rh5BG011400 Rh5DG092900 Rh6AG434000 Rh6CG153300 Rh6CG391700 Rh7BG333600
rosa_wichuraiana Rw0G005570 Rw0G005590 Rw0G011850 Rw0G015530 Rw0G017350 Rw0G019580 Rw3G000600 Rw3G024160 Rw4G023620 Rw4G023640 Rw4G023660 Rw4G023670 Rw4G023950 Rw4G023960 Rw4G024050 Rw4G024060 Rw5G048020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 686, 810, 1068, 2255
AasI GACNNNNNNGTC 1 cut(s) 1947
Acc36I ACCTGC 1 cut(s) 2567
AccB1I GGYRCC 1 cut(s) 206
AccBSI CCGCTC 2 cut(s) 3228, 3267
AccI GTMKAC 1 cut(s) 2898
AccII CGCG 3 cut(s) 2145, 3429, 3553
AccIII TCCGGA 1 cut(s) 3310
AciI CCGC 6 cut(s) 1316, 1505, 2140, 2143, 3226, 3267
AclWI GGATC 9 cut(s) 96, 283, 1009, 1307, 1826, 1980, 2317, 2344, 3302
AcoI YGGCCR 2 cut(s) 3083, 3495
AcsI RAATTY 8 cut(s) 286, 326, 554, 1358, 1511, 1819, 1999, 3003
AcuI CTGAAG 2 cut(s) 1491, 2799
AfaI GTAC 4 cut(s) 972, 1750, 2664, 3118
AfiI CCNNNNNNNGG 8 cut(s) 241, 343, 1322, 2231, 2360, 3035, 3317, 3655
AflII CTTAAG 1 cut(s) 1990
AflIII ACRYGT 1 cut(s) 3538
AjnI CCWGG 5 cut(s) 94, 336, 369, 2246, 3246
AjuI GAANNNNNNNTTGG 2 cut(s) 735, 767
Alw21I GWGCWC 5 cut(s) 1660, 2241, 2926, 3607, 3824
Alw26I GTCTC 8 cut(s) 304, 322, 439, 1143, 1298, 2137, 2526, 3201
Alw44I GTGCAC 2 cut(s) 1656, 3603
AlwI GGATC 9 cut(s) 96, 283, 1009, 1307, 1826, 1980, 2317, 2344, 3302
AlwNI CAGNNNCTG 4 cut(s) 863, 1673, 2381, 2999
Aor13HI TCCGGA 1 cut(s) 3310
AoxI GGCC 6 cut(s) 1534, 2520, 2818, 3083, 3185, 3495
ApaI GGGCCC 1 cut(s) 2524
ApaLI GTGCAC 2 cut(s) 1656, 3603
ApoI RAATTY 8 cut(s) 286, 326, 554, 1358, 1511, 1819, 1999, 3003
AseI ATTAAT 1 cut(s) 3774
Asp700I GAANNNNTTC 3 cut(s) 135, 2002, 2684
AspLEI GCGC 3 cut(s) 2693, 3431, 3553
AspS9I GGNCC 6 cut(s) 756, 1534, 2520, 2521, 2819, 3026
AsuNHI GCTAGC 1 cut(s) 2107
AvaII GGWCC 2 cut(s) 756, 3026
AxyI CCTNAGG 2 cut(s) 1530, 1836
BaeGI GKGCMC 3 cut(s) 1660, 2524, 3607
BalI TGGCCA 1 cut(s) 3497
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 4 cut(s) 2524, 2926, 3572, 3824
BarI GAAGNNNNNNTAC 2 cut(s) 1987, 2019
BauI CACGAG 2 cut(s) 2358, 2930
BbsI GAAGAC 2 cut(s) 1908, 2834
Bbv12I GWGCWC 5 cut(s) 1660, 2241, 2926, 3607, 3824
BbvCI CCTCAGC 1 cut(s) 6
BceAI ACGGC 1 cut(s) 2007
BciT130I CCWGG 5 cut(s) 96, 338, 371, 2248, 3248
BciVI GTATCC 2 cut(s) 1238, 3186
BclI TGATCA 1 cut(s) 2850
BcoDI GTCTC 8 cut(s) 304, 322, 439, 1143, 1298, 2137, 2526, 3201
BfaI CTAG 7 cut(s) 108, 1233, 2108, 2525, 2540, 3183, 3803
BfmI CTRYAG 4 cut(s) 1059, 2007, 2193, 2568
BfrI CTTAAG 1 cut(s) 1990
BfuAI ACCTGC 1 cut(s) 2567
BfuI GTATCC 2 cut(s) 1238, 3186
BlpI GCTNAGC 1 cut(s) 70
BmcAI AGTACT 1 cut(s) 972
Bme1390I CCNGG 5 cut(s) 96, 338, 371, 2248, 3248
Bme18I GGWCC 2 cut(s) 756, 3026
BmgT120I GGNCC 6 cut(s) 756, 1534, 2520, 2521, 2819, 3026
BmiI GGNNCC 5 cut(s) 208, 757, 1407, 2522, 3028
BmrFI CCNGG 5 cut(s) 96, 338, 371, 2248, 3248
BmrI ACTGGG 1 cut(s) 969
BmtI GCTAGC 1 cut(s) 2111
BmuI ACTGGG 1 cut(s) 969
BpiI GAAGAC 2 cut(s) 1908, 2834
BplI GAGNNNNNCTC 4 cut(s) 53, 85, 2913, 2945
BpmI CTGGAG 2 cut(s) 353, 504
Bpu10I CCTNAGC 1 cut(s) 6
Bpu1102I GCTNAGC 1 cut(s) 70
BpuEI CTTGAG 1 cut(s) 2455
BsaBI GATNNNNATC 2 cut(s) 677, 3776
BsaI GGTCTC 3 cut(s) 322, 2137, 3201
BsaJI CCNNGG 8 cut(s) 534, 703, 912, 1625, 2247, 2326, 2988, 3246
BsaWI WCCGGW 3 cut(s) 1192, 3310, 3663
BsaXI ACNNNNNCTCC 8 cut(s) 339, 369, 477, 507, 2432, 2462, 3729, 3759
Bsc4I CCNNNNNNNGG 8 cut(s) 241, 343, 1322, 2231, 2360, 3035, 3317, 3655
Bse118I RCCGGY 1 cut(s) 3081
Bse1I ACTGG 9 cut(s) 487, 787, 964, 1003, 1409, 2062, 2174, 2907, 3004
Bse21I CCTNAGG 2 cut(s) 1530, 1836
Bse3DI GCAATG 5 cut(s) 118, 2586, 2643, 3435, 3546
Bse8I GATNNNNATC 2 cut(s) 677, 3776
BseAI TCCGGA 1 cut(s) 3310
BseBI CCWGG 5 cut(s) 96, 338, 371, 2248, 3248
BseDI CCNNGG 8 cut(s) 534, 703, 912, 1625, 2247, 2326, 2988, 3246
BseGI GGATG 7 cut(s) 390, 698, 1421, 1462, 1648, 3595, 3797
BseJI GATNNNNATC 2 cut(s) 677, 3776
BseLI CCNNNNNNNGG 8 cut(s) 241, 343, 1322, 2231, 2360, 3035, 3317, 3655
BseMI GCAATG 5 cut(s) 118, 2586, 2643, 3435, 3546
BseNI ACTGG 9 cut(s) 487, 787, 964, 1003, 1409, 2062, 2174, 2907, 3004
BseRI GAGGAG 3 cut(s) 23, 1211, 2729
BseSI GKGCMC 3 cut(s) 1660, 2524, 3607
BseYI CCCAGC 1 cut(s) 2353
Bsh1236I CGCG 3 cut(s) 2145, 3429, 3553
BshFI GGCC 6 cut(s) 1536, 2522, 2820, 3085, 3187, 3497
BshNI GGYRCC 1 cut(s) 206
BsiHKAI GWGCWC 5 cut(s) 1660, 2241, 2926, 3607, 3824
BsiSI CCGG 5 cut(s) 1193, 2919, 3082, 3311, 3664
BslFI GGGAC 2 cut(s) 3012, 3399
BslI CCNNNNNNNGG 8 cut(s) 241, 343, 1322, 2231, 2360, 3035, 3317, 3655
BsmAI GTCTC 8 cut(s) 304, 322, 439, 1143, 1298, 2137, 2526, 3201
BsmBI CGTCTC 1 cut(s) 2526
BsmFI GGGAC 2 cut(s) 3012, 3399
BsmI GAATGC 3 cut(s) 599, 1765, 3712
BsnI GGCC 6 cut(s) 1536, 2522, 2820, 3085, 3187, 3497
Bso31I GGTCTC 3 cut(s) 322, 2137, 3201
Bsp120I GGGCCC 1 cut(s) 2520
Bsp1286I GDGCHC 7 cut(s) 1660, 2241, 2524, 2926, 3572, 3607, 3824
Bsp13I TCCGGA 1 cut(s) 3310
Bsp1407I TGTACA 1 cut(s) 2662
Bsp1720I GCTNAGC 1 cut(s) 70
Bsp19I CCATGG 2 cut(s) 534, 2988
BspACI CCGC 6 cut(s) 1316, 1505, 2140, 2143, 3226, 3267
BspANI GGCC 6 cut(s) 1536, 2522, 2820, 3085, 3187, 3497
BspEI TCCGGA 1 cut(s) 3310
BspFNI CGCG 3 cut(s) 2145, 3429, 3553
BspHI TCATGA 3 cut(s) 40, 237, 2676
BspLI GGNNCC 5 cut(s) 208, 757, 1407, 2522, 3028
BspMAI CTGCAG 2 cut(s) 1063, 2572
BspMI ACCTGC 1 cut(s) 2567
BspOI GCTAGC 1 cut(s) 2111
BspPI GGATC 9 cut(s) 96, 283, 1009, 1307, 1826, 1980, 2317, 2344, 3302
BspQI GCTCTTC 2 cut(s) 3816, 3829
BspT107I GGYRCC 1 cut(s) 206
BspTI CTTAAG 1 cut(s) 1990
BspTNI GGTCTC 3 cut(s) 322, 2137, 3201
BsrBI CCGCTC 2 cut(s) 3228, 3267
BsrDI GCAATG 5 cut(s) 118, 2586, 2643, 3435, 3546
BsrFI RCCGGY 1 cut(s) 3081
BsrGI TGTACA 1 cut(s) 2662
BsrI ACTGG 9 cut(s) 487, 787, 964, 1003, 1409, 2062, 2174, 2907, 3004
BssAI RCCGGY 1 cut(s) 3081
BssECI CCNNGG 8 cut(s) 534, 703, 912, 1625, 2247, 2326, 2988, 3246
BssSI CACGAG 2 cut(s) 2358, 2930
BssT1I CCWWGG 5 cut(s) 534, 912, 1625, 2326, 2988
Bst2BI CACGAG 2 cut(s) 2358, 2930
Bst2UI CCWGG 5 cut(s) 96, 338, 371, 2248, 3248
Bst6I CTCTTC 5 cut(s) 54, 1467, 2775, 3816, 3829
BstAFI CTTAAG 1 cut(s) 1990
BstAPI GCANNNNNTGC 5 cut(s) 665, 1046, 1664, 2486, 3611
BstAUI TGTACA 1 cut(s) 2662
BstC8I GCNNGC 9 cut(s) 1320, 1497, 1865, 2109, 2383, 2613, 2722, 2941, 3555
BstDSI CCRYGG 3 cut(s) 534, 703, 2988
BstF5I GGATG 7 cut(s) 390, 698, 1421, 1462, 1648, 3595, 3797
BstFNI CGCG 3 cut(s) 2145, 3429, 3553
BstHHI GCGC 3 cut(s) 2693, 3431, 3553
BstMAI GTCTC 8 cut(s) 304, 322, 439, 1143, 1298, 2137, 2526, 3201
BstNI CCWGG 5 cut(s) 96, 338, 371, 2248, 3248
BstNSI RCATGY 1 cut(s) 2604
BstSCI CCNGG 5 cut(s) 94, 336, 369, 2246, 3246
BstSFI CTRYAG 4 cut(s) 1059, 2007, 2193, 2568
BstSLI GKGCMC 3 cut(s) 1660, 2524, 3607
BstUI CGCG 3 cut(s) 2145, 3429, 3553
BstV2I GAAGAC 2 cut(s) 1908, 2834
BstX2I RGATCY 3 cut(s) 275, 1001, 1831
BstYI RGATCY 3 cut(s) 275, 1001, 1831
Bsu36I CCTNAGG 2 cut(s) 1530, 1836
BsuI GTATCC 2 cut(s) 1238, 3186
BsuRI GGCC 6 cut(s) 1536, 2522, 2820, 3085, 3187, 3497
BtgI CCRYGG 3 cut(s) 534, 703, 2988
BtgZI GCGATG 1 cut(s) 3798
BtsCI GGATG 7 cut(s) 390, 698, 1421, 1462, 1648, 3595, 3797
BtsI GCAGTG 2 cut(s) 2630, 3729
BtsIMutI CAGTG 9 cut(s) 480, 513, 1467, 1758, 2055, 2630, 2860, 3678, 3729
BveI ACCTGC 1 cut(s) 2567
Cac8I GCNNGC 9 cut(s) 1320, 1497, 1865, 2109, 2383, 2613, 2722, 2941, 3555
CaiI CAGNNNCTG 4 cut(s) 863, 1673, 2381, 2999
CciI TCATGA 3 cut(s) 40, 237, 2676
CfoI GCGC 3 cut(s) 2693, 3431, 3553
Cfr10I RCCGGY 1 cut(s) 3081
Cfr13I GGNCC 6 cut(s) 756, 1534, 2520, 2521, 2819, 3026
CseI GACGC 3 cut(s) 2499, 2543, 2747
Csp6I GTAC 4 cut(s) 971, 1749, 2663, 3117
CspCI CAANNNNNGTGG 4 cut(s) 2044, 2079, 3657, 3692
CviQI GTAC 4 cut(s) 971, 1749, 2663, 3117
DrdI GACNNNNNNGTC 1 cut(s) 1947
DseDI GACNNNNNNGTC 1 cut(s) 1947
EaeI YGGCCR 2 cut(s) 3083, 3495
Eam1104I CTCTTC 5 cut(s) 54, 1467, 2775, 3816, 3829
EarI CTCTTC 5 cut(s) 54, 1467, 2775, 3816, 3829
EciI GGCGGA 1 cut(s) 2129
Ecl136II GAGCTC 2 cut(s) 2924, 3822
Eco130I CCWWGG 5 cut(s) 534, 912, 1625, 2326, 2988
Eco24I GRGCYC 4 cut(s) 2524, 2926, 3572, 3824
Eco31I GGTCTC 3 cut(s) 322, 2137, 3201
Eco32I GATATC 1 cut(s) 2337
Eco47I GGWCC 2 cut(s) 756, 3026
Eco53kI GAGCTC 2 cut(s) 2924, 3822
Eco57I CTGAAG 2 cut(s) 1491, 2799
Eco81I CCTNAGG 2 cut(s) 1530, 1836
EcoICRI GAGCTC 2 cut(s) 2924, 3822
EcoO109I RGGNCCY 4 cut(s) 1534, 2520, 2521, 2819
EcoRII CCWGG 5 cut(s) 94, 336, 369, 2246, 3246
EcoRV GATATC 1 cut(s) 2337
EcoT14I CCWWGG 5 cut(s) 534, 912, 1625, 2326, 2988
EcoT22I ATGCAT 3 cut(s) 1034, 3643, 3799
EcoT38I GRGCYC 4 cut(s) 2524, 2926, 3572, 3824
ErhI CCWWGG 5 cut(s) 534, 912, 1625, 2326, 2988
Esp3I CGTCTC 1 cut(s) 2526
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FaqI GGGAC 2 cut(s) 3012, 3399
FauNDI CATATG 3 cut(s) 907, 2092, 3442
FbaI TGATCA 1 cut(s) 2850
FblI GTMKAC 1 cut(s) 2898
FokI GGATG 7 cut(s) 397, 685, 1408, 1469, 1655, 3602, 3784
FriOI GRGCYC 4 cut(s) 2524, 2926, 3572, 3824
FspBI CTAG 7 cut(s) 108, 1233, 2108, 2525, 2540, 3183, 3803
GlaI GCGC 3 cut(s) 2692, 3430, 3552
GsaI CCCAGC 1 cut(s) 2357
GsuI CTGGAG 2 cut(s) 353, 504
HaeIII GGCC 6 cut(s) 1536, 2522, 2820, 3085, 3187, 3497
HapII CCGG 5 cut(s) 1193, 2919, 3082, 3311, 3664
HgaI GACGC 3 cut(s) 2499, 2543, 2747
HhaI GCGC 3 cut(s) 2693, 3431, 3553
Hin6I GCGC 3 cut(s) 2691, 3429, 3551
HinP1I GCGC 3 cut(s) 2691, 3429, 3551
HincII GTYRAC 4 cut(s) 996, 1281, 1714, 2653
HindII GTYRAC 4 cut(s) 996, 1281, 1714, 2653
HindIII AAGCTT 1 cut(s) 3166
HpaI GTTAAC 2 cut(s) 1714, 2653
HpaII CCGG 5 cut(s) 1193, 2919, 3082, 3311, 3664
Hpy99I CGWCG 1 cut(s) 2515
HpyAV CCTTC 7 cut(s) 538, 845, 2674, 2779, 2799, 2810, 2929
HpyCH4IV ACGT 3 cut(s) 1895, 2961, 3538
HpySE526I ACGT 3 cut(s) 1895, 2961, 3538
HspAI GCGC 3 cut(s) 2691, 3429, 3551
Kpn2I TCCGGA 1 cut(s) 3310
Ksp22I TGATCA 1 cut(s) 2850
KspAI GTTAAC 2 cut(s) 1714, 2653
LguI GCTCTTC 2 cut(s) 3816, 3829
LmnI GCTCC 6 cut(s) 347, 1198, 1841, 2921, 3023, 3264
MaeI CTAG 7 cut(s) 108, 1233, 2108, 2525, 2540, 3183, 3803
MaeII ACGT 3 cut(s) 1895, 2961, 3538
MaeIII GTNAC 5 cut(s) 388, 509, 1136, 2024, 3104
MbiI CCGCTC 2 cut(s) 3228, 3267
MfeI CAATTG 2 cut(s) 750, 2481
MflI RGATCY 3 cut(s) 275, 1001, 1831
MhlI GDGCHC 7 cut(s) 1660, 2241, 2524, 2926, 3572, 3607, 3824
MlsI TGGCCA 1 cut(s) 3497
MluNI TGGCCA 1 cut(s) 3497
MlyI GAGTC 6 cut(s) 1892, 1957, 1972, 3119, 3381, 3660
MmeI TCCRAC 7 cut(s) 529, 1237, 2167, 2509, 2725, 3232, 3567
Mox20I TGGCCA 1 cut(s) 3497
Mph1103I ATGCAT 3 cut(s) 1034, 3643, 3799
MroI TCCGGA 1 cut(s) 3310
MroXI GAANNNNTTC 3 cut(s) 135, 2002, 2684
MscI TGGCCA 1 cut(s) 3497
MslI CAYNNNNRTG 1 cut(s) 1027
Msp20I TGGCCA 1 cut(s) 3497
MspA1I CMGCKG 6 cut(s) 353, 565, 860, 2501, 2567, 2611
MspCI CTTAAG 1 cut(s) 1990
MspI CCGG 5 cut(s) 1193, 2919, 3082, 3311, 3664
MspR9I CCNGG 5 cut(s) 96, 338, 371, 2248, 3248
MunI CAATTG 2 cut(s) 750, 2481
Mva1269I GAATGC 3 cut(s) 599, 1765, 3712
MvaI CCWGG 5 cut(s) 96, 338, 371, 2248, 3248
MvnI CGCG 3 cut(s) 2145, 3429, 3553
NcoI CCATGG 2 cut(s) 534, 2988
NdeI CATATG 3 cut(s) 907, 2092, 3442
NheI GCTAGC 1 cut(s) 2107
NlaIV GGNNCC 5 cut(s) 208, 757, 1407, 2522, 3028
NmeAIII GCCGAG 1 cut(s) 1516
NmuCI GTSAC 4 cut(s) 388, 509, 1136, 2024
NsiI ATGCAT 3 cut(s) 1034, 3643, 3799
NspI RCATGY 1 cut(s) 2604
PagI TCATGA 3 cut(s) 40, 237, 2676
PciSI GCTCTTC 2 cut(s) 3816, 3829
PctI GAATGC 3 cut(s) 599, 1765, 3712
PdmI GAANNNNTTC 3 cut(s) 135, 2002, 2684
PfeI GAWTC 9 cut(s) 136, 266, 1375, 1670, 2170, 2685, 3032, 3314, 3617
PflFI GACNNNGTC 2 cut(s) 296, 3650
PleI GAGTC 6 cut(s) 1891, 1956, 1971, 3118, 3381, 3659
PpsI GAGTC 6 cut(s) 1891, 1956, 1971, 3118, 3381, 3659
PshBI ATTAAT 1 cut(s) 3774
PsiI TTATAA 4 cut(s) 686, 810, 1068, 2255
Psp124BI GAGCTC 2 cut(s) 2926, 3824
Psp6I CCWGG 5 cut(s) 94, 336, 369, 2246, 3246
PspFI CCCAGC 1 cut(s) 2353
PspGI CCWGG 5 cut(s) 94, 336, 369, 2246, 3246
PspN4I GGNNCC 5 cut(s) 208, 757, 1407, 2522, 3028
PspOMI GGGCCC 1 cut(s) 2520
PspPI GGNCC 6 cut(s) 756, 1534, 2520, 2521, 2819, 3026
PstI CTGCAG 2 cut(s) 1063, 2572
PstNI CAGNNNCTG 4 cut(s) 863, 1673, 2381, 2999
PsuI RGATCY 3 cut(s) 275, 1001, 1831
PsyI GACNNNGTC 2 cut(s) 296, 3650
PvuII CAGCTG 6 cut(s) 353, 565, 860, 2501, 2567, 2611
RsaI GTAC 4 cut(s) 972, 1750, 2664, 3118
RsaNI GTAC 4 cut(s) 971, 1749, 2663, 3117
RseI CAYNNNNRTG 1 cut(s) 1027
SacI GAGCTC 2 cut(s) 2926, 3824
SapI GCTCTTC 2 cut(s) 3816, 3829
Sau96I GGNCC 6 cut(s) 756, 1534, 2520, 2521, 2819, 3026
ScaI AGTACT 1 cut(s) 972
SchI GAGTC 6 cut(s) 1892, 1957, 1972, 3119, 3381, 3660
ScrFI CCNGG 5 cut(s) 96, 338, 371, 2248, 3248
SduI GDGCHC 7 cut(s) 1660, 2241, 2524, 2926, 3572, 3607, 3824
SfcI CTRYAG 4 cut(s) 1059, 2007, 2193, 2568
SinI GGWCC 2 cut(s) 756, 3026
SmiMI CAYNNNNRTG 1 cut(s) 1027
SmlI CTYRAG 2 cut(s) 1990, 2470
SmoI CTYRAG 2 cut(s) 1990, 2470
SsiI CCGC 6 cut(s) 1316, 1505, 2140, 2143, 3226, 3267
SspI AATATT 4 cut(s) 441, 1206, 1441, 2229
SspMI CTAG 7 cut(s) 108, 1233, 2108, 2525, 2540, 3183, 3803
SstI GAGCTC 2 cut(s) 2926, 3824
StyD4I CCNGG 5 cut(s) 94, 336, 369, 2246, 3246
StyI CCWWGG 5 cut(s) 534, 912, 1625, 2326, 2988
TaiI ACGT 3 cut(s) 1898, 2964, 3541
TaqI TCGA 3 cut(s) 465, 1946, 1961
TaqII GACCGA 1 cut(s) 3663
TatI WGTACW 3 cut(s) 970, 1748, 2662
TauI GCSGC 2 cut(s) 1508, 2145
TfiI GAWTC 9 cut(s) 136, 266, 1375, 1670, 2170, 2685, 3032, 3314, 3617
TscAI CASTG 9 cut(s) 487, 520, 1474, 1758, 2062, 2637, 2860, 3678, 3729
TseFI GTSAC 4 cut(s) 388, 509, 1136, 2024
Tsp45I GTSAC 4 cut(s) 388, 509, 1136, 2024
TspGWI ACGGA 1 cut(s) 2670
TspRI CASTG 9 cut(s) 487, 520, 1474, 1758, 2062, 2637, 2860, 3678, 3729
Tth111I GACNNNGTC 2 cut(s) 296, 3650
Vha464I CTTAAG 1 cut(s) 1990
VneI GTGCAC 2 cut(s) 1656, 3603
VpaK11BI GGWCC 2 cut(s) 756, 3026
VspI ATTAAT 1 cut(s) 3774
XapI RAATTY 8 cut(s) 286, 326, 554, 1358, 1511, 1819, 1999, 3003
XceI RCATGY 1 cut(s) 2604
XmiI GTMKAC 1 cut(s) 2898
XmnI GAANNNNTTC 3 cut(s) 135, 2002, 2684
XspI CTAG 7 cut(s) 108, 1233, 2108, 2525, 2540, 3183, 3803
ZrmI AGTACT 1 cut(s) 972
Zsp2I ATGCAT 3 cut(s) 1034, 3643, 3799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.