Rroxscaffold_5G00368970

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000116
Physical Location & Seq
Reverse (-)
50728321 .. 50775462
47142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00368970.1

Sequence Viewer

Length: 3813 bp
ATGGCATCAACAAAGGCAGCAGATTCCGAAAACAACAAGCAGGATACTGCAGGCATGAGCAAGGAGGGTGCAACCAACACAGTACCATATTACAAACTCTTCTCATTTGCTGATTCCCTGGATTACCTGTTAATGTCTGTTGGTACAATCAGTGCTATCGGAAATGGACTATGTATGCCTCTAATGACCGTGATCATGGGGGAGGTGATCAACTCTTTTGGAGAAACAACGAGTATCAATAAAGTGGTTGGTATAGTTTCCCAGGTGGCTCTAAAGTACGTTTACTTGGCTCTGGGGGCTGCTGCTGCTGCATTTTTACAAATGTCTTGCTGGATGATCACTGGAGAGAGACAGGCAGCAAGAATAAGAAGTTTATACTTGAAAACAATACTAAGGCAAGACGTTGGCTTTTTTGATACAGAGGCTAACACTGGGGAAATTGTTGGCAGGATGTCGGGGGATACTGTGCTCATTCAGGAAGCGATGGGGGAGAAAGTAGGAAGTTTTATCCAGTTAGTTGCTACATTCATAGGAGGCTTTGTTATAGCATTTGCCAAGGGATGGCGTCTCACCCTTGTCATGCTAACCTCTATTCCCCCTCTTATCTTATCCGGTGCTGTCATGAGCCTCACTATAAGGAAGTTGGCGTCCCGTGGACAAGAGGCATATTCAGTTGGAGCAACTGTTGTAGAGCAGACAATTGGTTCTATCAGAACTGTTGCGTCATTTACGGGGGAGAAGCAAGCTATAACTAAGTACAACAACTCCTTAACTAAAGCTTACAAGTCTGGTGTGCAAGAGGGCTTAGCATCAGGAGTGGGTCTTGGTACGGTTATTCTTACTTTATTCTGCAGTTATGCATTGGCTATATGGTATGGCGGCAAAATGATACTTGAAAAGGGGTACAAAGCAGGAGATGTCATCAATGTGATTTTTGCTGTATTCACTGGTTCTTTCTCCCTCGGGCAGGCATCTCCATGCGTGAGTGCATTTTCTGCGGGACAAGCTGCAGCATATAAGATGTTTGAGACAATCAACAGAAAGCCAGATATAGATGTGTATGACACCAGAGGACTGAAACCACAAGAGATCCGCGGAGACATTGAACTGAGGGATGTTTATTTTAGTTATCCTGCAAGACCGGATGAGCAAATATTCCTCGGATTTTCTCTTTCGATACCTAGTGGTGCAACTGTTGCTTTGGTTGGACAAAGCGGAAGTGGTAAATCAACTGTCATCAGTTTGATTGAGAGATTTTATGATCCCCAGGCTGGTGAAGTTCTTATTGATGGGAATAATCTAAAAGAGTTTCAACTGAAATGGATCAGACAGAAAATAGGCCTTGTAAGCCAGGAACCAGTTTTGTTTACTGGTAGCATTAAAGATAATATTGGATATGGAAAGAATGATGCAACAACTGAAGAAATGAGGGCTGCTGCTGAGCTGGCCAATGCTGCAAAATTCATAGACAAACTACCTCAGGGACTAGATACAATGGTTGGTGAACATGGAACTCAGCTATCTGGGGGCCAAAAGCAGAGAATTGCCATAGCAAGAGCAATTCTGAAGAACCCAAGAATTCTGCTTTTGGATGAAGCAACAAGCGCTCTTGATGCAGAATCTGAGAGAGTTGTGCAAGAGGCATTGGACAGAATTATGGTTAACAGGACTACTGTCATTGTAGCCCATCGCTTGAGCACAGTAAGGACTGCTGATACAATTGCAGTAATACATCACGGAAAAATTGTTGAAAAAGGACCACATACTGAGCTGGTTAAGGATCCTGATGGTGCATATAGCCAACTTATAAGATTGCAAGAAATTAGCAGTGTGTCAGAACATGATGCTCAAAATGATCATGAAAGGCCAGAACTTTCCTCAAGTTTTCGGGAACTTTCTTCCTTTAGATCCATAAGTCGGGGGTCATCAGAAATAGGAAATAGTAGTCACCGTAGTCACTCATTTTCAATCTCACATGGTGCTCCTACTGTAACTGGTGTCTTTGAAATAGCTCCTGCAGAACCTGACATTCCAGCTGCATCGATAGAAGTGCATCTGGAAGTCTCACTTAGCCGCCTGGCTTATCTGAACAAGACAGAAATCCCAGTACTACTTCTAGGTTCTATAGCTGCAGCAATCTGTGGCGTAATCTTGCCTATTTTTGGGGTGCTAATATCTAGCATAATCAAAACCTTCTTTGATCCACCTCATCAGCTCCGAAAGGACTCAAAATTTTGGGCATTAATCTTTGTTGTTATTGGAGTGGTAGCTCTCTTGGCGCATCCGGTGAGAGCATACTTCTTTGCTGTGGCAGGGTGTAAGTTAATAAGACGAATCCGATCACTATGCTTTGAGAAGGTCATTTATATGGAAGCAAGTTGGTTTGATGAAGCCGAGCACTCGAGTGGTGCACTTGGTGCAAGGCTTTCAGGAAATGCAGCTTCTCTGCGAGGGCTGGTTGGAGATGCTCTCAGTTTGGCTGTTATGCATGCATCAACTACAATTGCTGGCTTGGTTATTGCTTTTTTGGCAAACTGGCAACTTGCTTTTATTATTCTTCTTATGCTGCCTCTACTAGGAATAAGTGGATATTTTCAAATCGAGGTCCTGAAAGGATTAAGCTCAAATGCAAAGAAAATGTATGAAGATGCAAGCCAAGTAGCCACCAATGCTGTGGGGAGTATTAGAACAATTGCTTCCTTTTGTGCTGAGGAAAAGATTATTAAGTTGTACCAGACAAAATGTGAAGGTCCTATCAAGGCAGGGATAAGACAAGGTATAGTTAGTGGAATAGGCTTTGGGCTATCACTCTTCTTGCTCTTTTCTGTATATGCATGCAGTTTCTATGCAGGGGCTCGACTTGTTGGAGCAGGCAAGACGACATTCCCAGATGTTTTTCGGGTTTTCTTTGCCCTCACTATGACAGCTGTGGCACTGTCTCAACAGGGTTCCTTAGCCCCTGATGCAAGCAAGGGAAAGAGCTCTGCTGCTTCAATATTTGCAATTCTTGACCGGAAATCAAAAATAGATGCTAGTGATAACTCCGGAACAACCATAGAAAATTTGAAGGGAGAAATTGAATTTTGCCATGTTAGTTTCAAGTATCCAAATAGACCTAATGTACCAATCTTCCAGGATCTGTGCTTGACCATTCACTATGGGCAGACAGTTGCTCTGGTTGGAGAAAGCGGAAGTGGGAAATCAACAGTGATCTCATTGCTGCAGAGATTTTATGATCCTGATTCAGGTCACATTACATTAGATGGTACTGAAATGCAAACATTACAGCTGAAGTGGTTGAGGCAGCAAATGGGGCTGGTGAGCCAGGAGCCTCTGCTTTTTAATGACACTATCAGAGCCAACATTGCATATGGAAAGGAAGGGAGTGCAACCGAGGCTGAAATTATAGCTGCAGCAGAATTGGCAAATGCTCACAAGTTCATTAGTAGTTTACAACAGGGCTATGATACAATAGTAGGTGAGCGAGGGGTCCAACTCTCTGGTGGACAGAAGCAAAGGGTAGCTATTGCAAGAGCTATAATGAAGGCACCAAAGATATTACTACTAGATGAAGCCACCAGTGCTCTTGATGCTGAATCTGAAAGAGTGGTTCAAGATGCACTGGATCGAGTTATGGTGGATCGAACTACAGTGGTGGTTGCCCATCGGCTATCCACAATTAATGGTGCAGATTTAATAGCAGTGGTGAAAAATGGAGTCATTGCAGAGAAAGGAAAGCATGAAACTCTGATCGGTATCAAGGGTGGAACTTATGCTTCTTTGGTAGCACTACATGCAAGTGCCTCGTCTTAG

Protein Analysis

1270

Amino Acids

136.45

Weight (kDa)

7.31

Isoelectric Point (pI)

32.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 47 - 319 9.4e-60 ABC transporter transmembrane region
ABC_tran PF00005 389 - 535 2.2e-34 ABC transporter
ABC_membrane PF00664 705 - 977 3.1e-53 ABC transporter transmembrane region
ABC_tran PF00005 1045 - 1194 4.6e-35 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02520 AT1G02520 AT1G02520 AT1G02530 AT1G02530 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT2G47000 AT3G62150 AT3G62150 AT3G62150 AT4G01820 AT4G01820 AT4G01830 AT4G01830
fragaria_vesca FvH4_1g26280 FvH4_4g21370 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21380 FvH4_4g21381 FvH4_4g21600 FvH4_4g21600 FvH4_4g21600 FvH4_4g21620 FvH4_4g21621 FvH4_4g21622 FvH4_6g00641 FvH4_6g00650
malus_domestica MD09G1290100.v1.1 MD13G1138600.v1.1 MD13G1138700.v1.1 MD13G1139100.v1.1 MD16G1134500.v1.1 MD16G1134700.v1.1 MD16G1135000.v1.1 MD16G1135100.v1.1 MD17G1284300.v1.1 MD17G1284700.v1.1
prunus_persica Prupe.1G103800_v2.0.a1 Prupe.1G103800_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G103900_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104100_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104300_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.1G104400_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.2G166500_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G003100_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123700_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1 Prupe.3G123800_v2.0.a1
pyrus_communis pycom04g08750 pycom09g19770 pycom09g19790 pycom13g09140 pycom16g11900 pycom16g11920 pycom16g11930 pycom17g28030
rosa_chinensis RchiOBHm_Chr2g0157341 RchiOBHm_Chr3g0448061 RchiOBHm_Chr3g0485931 RchiOBHm_Chr3g0485961 RchiOBHm_Chr4g0427151 RchiOBHm_Chr4g0427161 RchiOBHm_Chr4g0427211 RchiOBHm_Chr4g0427241 RchiOBHm_Chr4g0427271 RchiOBHm_Chr4g0427711 RchiOBHm_Chr4g0427721 RchiOBHm_Chr4g0427771 RchiOBHm_Chr4g0427781 RchiOBHm_Chr4g0427791 RchiOBHm_Chr4g0427851 RchiOBHm_Chr4g0427861 RchiOBHm_Chr4g0446561 RchiOBHm_Chr5g0081601 RchiOBHm_Chr6g0278251 RchiOBHm_Chr6g0303241
rosa_laevigata RLG00000007206 RLG00000007207 RLG00000007209 RLG00000007210 RLG00000007211 RLG00000007212 RLG00000007214 RLG00000007255 RLG00000007256 RLG00000007257 RLG00000011097 RLG00000019257 RLG00000023121 RLG00000025948 RLG00000027246 RLG00000029989
rosa_multiflora Rmu_co8076196.1_g000001 Rmu_co8517461.1_g000001 Rmu_sc0000066.1_g000018 Rmu_sc0000066.1_g000024 Rmu_sc0000066.1_g000033 Rmu_sc0000066.1_g000047 Rmu_sc0000066.1_g000048 Rmu_sc0001651.1_g000024 Rmu_sc0002816.1_g000003 Rmu_sc0003337.1_g000015 Rmu_sc0003444.1_g000015 Rmu_sc0003813.1_g000004 Rmu_sc0004876.1_g000001 Rmu_sc0005969.1_g000025 Rmu_sc0008650.1_g000006 Rmu_sc0008855.1_g000001 Rmu_sc0009165.1_g000001 Rmu_sc0009165.1_g000002 Rmu_sc0010010.1_g000001 Rmu_sc0010010.1_g000005 Rmu_sc0036078.1_g000001
rosa_roxburghii Rroxscaffold_2G00130200 Rroxscaffold_5G00367590 Rroxscaffold_5G00368970 Rroxscaffold_5G00368990 Rroxscaffold_5G00369340 Rroxscaffold_5G00369350 Rroxscaffold_5G00369380 Rroxscaffold_5G00369390 Rroxscaffold_5G00369440 Rroxscaffold_5G00369450 Rroxscaffold_5G00369500 Rroxscaffold_5G00369510 Rroxscaffold_6G00396190 Rroxscaffold_6G00426220 Rroxscaffold_6G00427790 Rroxscaffold_7G00164940
rosa_rugosa Rorug01G0160800.1 Rorug02G0341400 Rorug02G0461800 Rorug02G0608000 Rorug03G0222700 Rorug03G0222800 Rorug03G0222900 Rorug04G0216400 Rorug04G0216400 Rorug04G0220100 Rorug04G0220100 Rorug04G0220100 Rorug05G0181800 Rorug05G0409700
rosa_samantha Rh1BG177300 Rh1DG206800 Rh2AG590600 Rh2BG473400 Rh2DG482300 Rh3AG007500 Rh3AG272400 Rh3BG007200 Rh3BG307000 Rh3DG301600 Rh3DG301900 Rh4AG272300 Rh4AG272400 Rh4AG272700 Rh4AG275900 Rh4AG276100 Rh4AG276200 Rh4AG276500 Rh4AG276600 Rh4AG276700 Rh4AG277000 Rh4BG281900 Rh4BG282200 Rh4CG292900 Rh4CG293300 Rh4CG293400 Rh4CG293600 Rh4CG297400 Rh4CG297500 Rh4CG297900 Rh4CG298000 Rh4CG298300 Rh4CG298400 Rh4DG165900 Rh5BG011400 Rh5DG092900 Rh6AG434000 Rh6CG153300 Rh6CG391700 Rh7BG333600
rosa_wichuraiana Rw0G005570 Rw0G005590 Rw0G011850 Rw0G015530 Rw0G017350 Rw0G019580 Rw3G000600 Rw3G024160 Rw4G023620 Rw4G023640 Rw4G023660 Rw4G023670 Rw4G023950 Rw4G023960 Rw4G024050 Rw4G024060 Rw5G048020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1808
AccB1I GGYRCC 1 cut(s) 3547
AccB7I CCANNNNNTGG 1 cut(s) 561
AccII CGCG 1 cut(s) 1095
AccIII TCCGGA 1 cut(s) 3044
AciI CCGC 7 cut(s) 879, 998, 1093, 1095, 1215, 2074, 3189
AcoI YGGCCR 1 cut(s) 1446
AcsI RAATTY 5 cut(s) 1460, 1578, 2231, 3061, 3080
AcuI CTGAAG 3 cut(s) 1440, 1586, 3311
AcyI GRCGYC 2 cut(s) 565, 647
AdeI CACNNNGTG 2 cut(s) 1979, 2417
AfeI AGCGCT 1 cut(s) 1606
AfiI CCNNNNNNNGG 7 cut(s) 561, 967, 1271, 1917, 2162, 2576, 3245
AjnI CCWGG 7 cut(s) 117, 261, 1266, 1350, 2076, 3132, 3324
AleI CACNNNNGTG 1 cut(s) 2403
Alw21I GWGCWC 7 cut(s) 471, 1700, 1984, 2400, 2413, 2984, 3586
Alw26I GTCTC 6 cut(s) 343, 572, 1022, 1092, 2068, 2943
Alw44I GTGCAC 1 cut(s) 2409
AlwNI CAGNNNCTG 3 cut(s) 1622, 2024, 3139
Ama87I CYCGRG 2 cut(s) 962, 2401
Aor13HI TCCGGA 1 cut(s) 3044
Aor51HI AGCGCT 1 cut(s) 1606
AoxI GGCC 4 cut(s) 1339, 1446, 1528, 1865
ApaLI GTGCAC 1 cut(s) 2409
ApoI RAATTY 5 cut(s) 1460, 1578, 2231, 3061, 3080
AseI ATTAAT 2 cut(s) 2243, 3681
AspLEI GCGC 2 cut(s) 1607, 2281
AspS9I GGNCC 5 cut(s) 1528, 1757, 2605, 2750, 3490
AsuHPI GGTGA 9 cut(s) 217, 562, 1286, 1514, 1940, 2299, 3331, 3491, 3718
AvaI CYCGRG 2 cut(s) 962, 2401
AvaII GGWCC 4 cut(s) 1757, 2605, 2750, 3490
AxyI CCTNAGG 1 cut(s) 1479
BaeGI GKGCMC 1 cut(s) 2413
BaeI ACNNNNGTAYC 4 cut(s) 1482, 1515, 3459, 3492
BalI TGGCCA 1 cut(s) 1448
BamHI GGATCC 1 cut(s) 1780
BanI GGYRCC 1 cut(s) 3547
BanII GRGCYC 2 cut(s) 2857, 2984
Bbv12I GWGCWC 7 cut(s) 471, 1700, 1984, 2400, 2413, 2984, 3586
BbvCI CCTCAGC 1 cut(s) 2709
BccI CCATC 7 cut(s) 478, 555, 1283, 1695, 1781, 3257, 3672
BcgI CGANNNNNNTGC 5 cut(s) 2032, 2066, 2328, 2362, 3786
BciT130I CCWGG 7 cut(s) 119, 263, 1268, 1352, 2078, 3134, 3326
BciVI GTATCC 3 cut(s) 37, 454, 3114
BclI TGATCA 4 cut(s) 192, 207, 336, 1855
BcoDI GTCTC 6 cut(s) 343, 572, 1022, 1092, 2068, 2943
BfaI CTAG 7 cut(s) 1182, 1487, 2117, 2178, 2576, 3033, 3566
BfmI CTRYAG 9 cut(s) 48, 850, 1008, 2016, 2124, 2130, 3221, 3411, 3648
BfoI RGCGCY 1 cut(s) 1608
BfuI GTATCC 3 cut(s) 37, 454, 3114
BlpI GCTNAGC 2 cut(s) 805, 1440
BmcAI AGTACT 1 cut(s) 2109
Bme1390I CCNGG 7 cut(s) 119, 263, 1268, 1352, 2078, 3134, 3326
Bme18I GGWCC 4 cut(s) 1757, 2605, 2750, 3490
BmeT110I CYCGRG 2 cut(s) 962, 2401
BmgT120I GGNCC 5 cut(s) 1528, 1757, 2605, 2750, 3490
BmiI GGNNCC 7 cut(s) 1356, 1529, 1782, 2950, 3330, 3491, 3549
BmrFI CCNGG 7 cut(s) 119, 263, 1268, 1352, 2078, 3134, 3326
BmrI ACTGGG 2 cut(s) 441, 2099
BmuI ACTGGG 2 cut(s) 441, 2099
BoxI GACNNNNGTC 1 cut(s) 1673
BplI GAGNNNNNCTC 2 cut(s) 2454, 2486
BpmI CTGGAG 1 cut(s) 363
Bpu10I CCTNAGC 2 cut(s) 2709, 2953
Bpu1102I GCTNAGC 2 cut(s) 805, 1440
BpuEI CTTGAG 2 cut(s) 1714, 1864
Bsa29I ATCGAT 1 cut(s) 2042
BsaBI GATNNNNATC 1 cut(s) 3755
BsaHI GRCGYC 2 cut(s) 565, 647
BsaJI CCNNGG 9 cut(s) 117, 261, 555, 652, 961, 1093, 1159, 1266, 3393
BsaWI WCCGGW 5 cut(s) 611, 1141, 2284, 3012, 3044
BsaXI ACNNNNNCTCC 6 cut(s) 525, 555, 669, 699, 749, 779
Bsc4I CCNNNNNNNGG 7 cut(s) 561, 967, 1271, 1917, 2162, 2576, 3245
Bse21I CCTNAGG 1 cut(s) 1479
Bse3DI GCAATG 3 cut(s) 3215, 3363, 3720
Bse8I GATNNNNATC 1 cut(s) 3755
BseAI TCCGGA 1 cut(s) 3044
BseBI CCWGG 7 cut(s) 119, 263, 1268, 1352, 2078, 3134, 3326
BseCI ATCGAT 1 cut(s) 2042
BseDI CCNNGG 9 cut(s) 117, 261, 555, 652, 961, 1093, 1159, 1266, 3393
BseGI GGATG 7 cut(s) 339, 456, 566, 1120, 1150, 1597, 2281
BseJI GATNNNNATC 1 cut(s) 3755
BseLI CCNNNNNNNGG 7 cut(s) 561, 967, 1271, 1917, 2162, 2576, 3245
BseMI GCAATG 3 cut(s) 3215, 3363, 3720
BseMII CTCAG 8 cut(s) 1100, 1431, 1493, 1529, 1614, 1758, 2485, 2700
BseSI GKGCMC 1 cut(s) 2413
BsgI GTGCAG 1 cut(s) 3708
Bsh1236I CGCG 1 cut(s) 1095
BshFI GGCC 4 cut(s) 1341, 1448, 1530, 1867
BshNI GGYRCC 1 cut(s) 3547
BshVI ATCGAT 1 cut(s) 2042
BsiHKAI GWGCWC 7 cut(s) 471, 1700, 1984, 2400, 2413, 2984, 3586
BsiHKCI CYCGRG 2 cut(s) 962, 2401
BsiSI CCGG 5 cut(s) 612, 1142, 2285, 3013, 3045
BslFI GGGAC 3 cut(s) 634, 1014, 1497
BslI CCNNNNNNNGG 7 cut(s) 561, 967, 1271, 1917, 2162, 2576, 3245
BsmAI GTCTC 6 cut(s) 343, 572, 1022, 1092, 2068, 2943
BsmBI CGTCTC 1 cut(s) 572
BsmFI GGGAC 3 cut(s) 634, 1014, 1497
BsnI GGCC 4 cut(s) 1341, 1448, 1530, 1867
BsoBI CYCGRG 2 cut(s) 962, 2401
Bsp1286I GDGCHC 8 cut(s) 471, 1700, 1984, 2400, 2413, 2857, 2984, 3586
Bsp13I TCCGGA 1 cut(s) 3044
Bsp1720I GCTNAGC 2 cut(s) 805, 1440
BspACI CCGC 7 cut(s) 879, 998, 1093, 1095, 1215, 2074, 3189
BspANI GGCC 4 cut(s) 1341, 1448, 1530, 1867
BspCNI CTCAG 8 cut(s) 1101, 1432, 1492, 1528, 1615, 1759, 2484, 2701
BspDI ATCGAT 1 cut(s) 2042
BspEI TCCGGA 1 cut(s) 3044
BspFNI CGCG 1 cut(s) 1095
BspHI TCATGA 2 cut(s) 621, 1858
BspLI GGNNCC 7 cut(s) 1356, 1529, 1782, 2950, 3330, 3491, 3549
BspMAI CTGCAG 7 cut(s) 52, 854, 1012, 2020, 2134, 3225, 3415
BspT107I GGYRCC 1 cut(s) 3547
BsrDI GCAATG 3 cut(s) 3215, 3363, 3720
BssECI CCNNGG 9 cut(s) 117, 261, 555, 652, 961, 1093, 1159, 1266, 3393
BssNI GRCGYC 2 cut(s) 565, 647
BssT1I CCWWGG 1 cut(s) 555
Bst2UI CCWGG 7 cut(s) 119, 263, 1268, 1352, 2078, 3134, 3326
Bst6I CTCTTC 2 cut(s) 104, 2816
BstACI GRCGYC 2 cut(s) 565, 647
BstAPI GCANNNNNTGC 4 cut(s) 995, 1196, 2417, 3794
BstDSI CCRYGG 2 cut(s) 652, 1093
BstENI CCTNNNNNAGG 3 cut(s) 965, 2574, 3243
BstF5I GGATG 7 cut(s) 339, 456, 566, 1120, 1150, 1597, 2281
BstFNI CGCG 1 cut(s) 1095
BstH2I RGCGCY 1 cut(s) 1608
BstHHI GCGC 2 cut(s) 1607, 2281
BstMAI GTCTC 6 cut(s) 343, 572, 1022, 1092, 2068, 2943
BstNI CCWGG 7 cut(s) 119, 263, 1268, 1352, 2078, 3134, 3326
BstNSI RCATGY 3 cut(s) 2492, 2838, 3797
BstPAI GACNNNNGTC 1 cut(s) 1673
BstSCI CCNGG 7 cut(s) 117, 261, 1266, 1350, 2076, 3132, 3324
BstSFI CTRYAG 9 cut(s) 48, 850, 1008, 2016, 2124, 2130, 3221, 3411, 3648
BstSLI GKGCMC 1 cut(s) 2413
BstUI CGCG 1 cut(s) 1095
BstX2I RGATCY 4 cut(s) 1089, 1780, 1907, 3136
BstXI CCANNNNNNTGG 2 cut(s) 2674, 3500
BstYI RGATCY 4 cut(s) 1089, 1780, 1907, 3136
Bsu15I ATCGAT 1 cut(s) 2042
Bsu36I CCTNAGG 1 cut(s) 1479
BsuI GTATCC 3 cut(s) 37, 454, 3114
BsuRI GGCC 4 cut(s) 1341, 1448, 1530, 1867
BsuTUI ATCGAT 1 cut(s) 2042
BtgI CCRYGG 2 cut(s) 652, 1093
BtgZI GCGATG 2 cut(s) 497, 1673
BtsCI GGATG 7 cut(s) 339, 456, 566, 1120, 1150, 1597, 2281
BtsI GCAGTG 2 cut(s) 1834, 3708
CaiI CAGNNNCTG 3 cut(s) 1622, 2024, 3139
CciI TCATGA 2 cut(s) 621, 1858
CfoI GCGC 2 cut(s) 1607, 2281
Cfr13I GGNCC 5 cut(s) 1528, 1757, 2605, 2750, 3490
Cfr42I CCGCGG 1 cut(s) 1096
ClaI ATCGAT 1 cut(s) 2042
CseI GACGC 3 cut(s) 554, 636, 711
DraIII CACNNNGTG 2 cut(s) 1979, 2417
EaeI YGGCCR 1 cut(s) 1446
Eam1104I CTCTTC 2 cut(s) 104, 2816
EarI CTCTTC 2 cut(s) 104, 2816
Ecl136II GAGCTC 1 cut(s) 2982
Eco130I CCWWGG 1 cut(s) 555
Eco147I AGGCCT 1 cut(s) 1341
Eco24I GRGCYC 2 cut(s) 2857, 2984
Eco47I GGWCC 4 cut(s) 1757, 2605, 2750, 3490
Eco47III AGCGCT 1 cut(s) 1606
Eco53kI GAGCTC 1 cut(s) 2982
Eco57I CTGAAG 3 cut(s) 1440, 1586, 3311
Eco81I CCTNAGG 1 cut(s) 1479
Eco88I CYCGRG 2 cut(s) 962, 2401
EcoICRI GAGCTC 1 cut(s) 2982
EcoNI CCTNNNNNAGG 3 cut(s) 965, 2574, 3243
EcoO109I RGGNCCY 2 cut(s) 2605, 2750
EcoRI GAATTC 1 cut(s) 1578
EcoRII CCWGG 7 cut(s) 117, 261, 1266, 1350, 2076, 3132, 3324
EcoT14I CCWWGG 1 cut(s) 555
EcoT22I ATGCAT 4 cut(s) 862, 2490, 2494, 2836
EcoT38I GRGCYC 2 cut(s) 2857, 2984
ErhI CCWWGG 1 cut(s) 555
Esp3I CGTCTC 1 cut(s) 572
FalI AAGNNNNNCTT 2 cut(s) 2052, 2084
FaqI GGGAC 3 cut(s) 634, 1014, 1497
FauI CCCGC 1 cut(s) 991
FauNDI CATATG 1 cut(s) 3370
FbaI TGATCA 4 cut(s) 192, 207, 336, 1855
FokI GGATG 7 cut(s) 346, 463, 573, 1127, 1157, 1604, 2268
FriOI GRGCYC 2 cut(s) 2857, 2984
FspBI CTAG 7 cut(s) 1182, 1487, 2117, 2178, 2576, 3033, 3566
GlaI GCGC 2 cut(s) 1606, 2280
GsuI CTGGAG 1 cut(s) 363
HaeII RGCGCY 1 cut(s) 1608
HaeIII GGCC 4 cut(s) 1341, 1448, 1530, 1867
HapII CCGG 5 cut(s) 612, 1142, 2285, 3013, 3045
HgaI GACGC 3 cut(s) 554, 636, 711
HhaI GCGC 2 cut(s) 1607, 2281
Hin1I GRCGYC 2 cut(s) 565, 647
Hin6I GCGC 2 cut(s) 1605, 2279
HinP1I GCGC 2 cut(s) 1605, 2279
HincII GTYRAC 1 cut(s) 1663
HindII GTYRAC 1 cut(s) 1663
HindIII AAGCTT 1 cut(s) 777
HinfI GANTC 8 cut(s) 23, 113, 1619, 2225, 2334, 3242, 3596, 3717
HpaI GTTAAC 1 cut(s) 1663
HpaII CCGG 5 cut(s) 612, 1142, 2285, 3013, 3045
HphI GGTGA 9 cut(s) 217, 562, 1286, 1514, 1940, 2299, 3331, 3491, 3718
Hpy166II GTNNAC 8 cut(s) 283, 656, 1368, 1505, 1663, 2411, 3452, 3506
Hpy8I GTNNAC 8 cut(s) 283, 656, 1368, 1505, 1663, 2411, 3452, 3506
HpyAV CCTTC 6 cut(s) 2203, 2350, 2741, 3061, 3374, 3538
HpyCH4IV ACGT 2 cut(s) 279, 402
HpySE526I ACGT 2 cut(s) 279, 402
Hsp92I GRCGYC 2 cut(s) 565, 647
HspAI GCGC 2 cut(s) 1605, 2279
Kpn2I TCCGGA 1 cut(s) 3044
Ksp22I TGATCA 4 cut(s) 192, 207, 336, 1855
KspAI GTTAAC 1 cut(s) 1663
KspI CCGCGG 1 cut(s) 1096
LmnI GCTCC 6 cut(s) 677, 1987, 2017, 2220, 2867, 3328
MaeI CTAG 7 cut(s) 1182, 1487, 2117, 2178, 2576, 3033, 3566
MaeII ACGT 2 cut(s) 279, 402
MaeIII GTNAC 4 cut(s) 1946, 1955, 1990, 3248
MboII GAAGA 8 cut(s) 91, 1433, 1579, 1890, 2549, 2657, 2803, 3121
MfeI CAATTG 4 cut(s) 699, 1719, 2502, 2691
MflI RGATCY 4 cut(s) 1089, 1780, 1907, 3136
MhlI GDGCHC 8 cut(s) 471, 1700, 1984, 2400, 2413, 2857, 2984, 3586
MlsI TGGCCA 1 cut(s) 1448
MluNI TGGCCA 1 cut(s) 1448
MlyI GAGTC 2 cut(s) 2219, 3726
MmeI TCCRAC 6 cut(s) 655, 1186, 2440, 2845, 3160, 3517
Mox20I TGGCCA 1 cut(s) 1448
Mph1103I ATGCAT 4 cut(s) 862, 2490, 2494, 2836
MroI TCCGGA 1 cut(s) 3044
MscI TGGCCA 1 cut(s) 1448
MslI CAYNNNNRTG 5 cut(s) 926, 976, 2366, 2403, 3798
Msp20I TGGCCA 1 cut(s) 1448
MspA1I CMGCKG 4 cut(s) 1095, 2036, 2927, 3289
MspI CCGG 5 cut(s) 612, 1142, 2285, 3013, 3045
MspR9I CCNGG 7 cut(s) 119, 263, 1268, 1352, 2078, 3134, 3326
MunI CAATTG 4 cut(s) 699, 1719, 2502, 2691
MvaI CCWGG 7 cut(s) 119, 263, 1268, 1352, 2078, 3134, 3326
MvnI CGCG 1 cut(s) 1095
NdeI CATATG 1 cut(s) 3370
NlaIV GGNNCC 7 cut(s) 1356, 1529, 1782, 2950, 3330, 3491, 3549
NmeAIII GCCGAG 1 cut(s) 2419
NmuCI GTSAC 3 cut(s) 1946, 1955, 3248
NsiI ATGCAT 4 cut(s) 862, 2490, 2494, 2836
NspI RCATGY 3 cut(s) 2492, 2838, 3797
OliI CACNNNNGTG 1 cut(s) 2403
PaeI GCATGC 2 cut(s) 2492, 2838
PaeR7I CTCGAG 1 cut(s) 2401
PagI TCATGA 2 cut(s) 621, 1858
PceI AGGCCT 1 cut(s) 1341
PfeI GAWTC 6 cut(s) 23, 113, 1619, 2334, 3242, 3596
PflMI CCANNNNNTGG 1 cut(s) 561
PfoI TCCNGGA 1 cut(s) 3132
PleI GAGTC 2 cut(s) 2219, 3725
PpsI GAGTC 2 cut(s) 2219, 3725
PpuMI RGGWCCY 2 cut(s) 2605, 2750
PshAI GACNNNNGTC 1 cut(s) 1673
PshBI ATTAAT 2 cut(s) 2243, 3681
PsiI TTATAA 1 cut(s) 1808
Psp124BI GAGCTC 1 cut(s) 2984
Psp5II RGGWCCY 2 cut(s) 2605, 2750
Psp6I CCWGG 7 cut(s) 117, 261, 1266, 1350, 2076, 3132, 3324
PspGI CCWGG 7 cut(s) 117, 261, 1266, 1350, 2076, 3132, 3324
PspN4I GGNNCC 7 cut(s) 1356, 1529, 1782, 2950, 3330, 3491, 3549
PspPI GGNCC 5 cut(s) 1528, 1757, 2605, 2750, 3490
PspPPI RGGWCCY 2 cut(s) 2605, 2750
PspXI VCTCGAGB 1 cut(s) 2401
PstI CTGCAG 7 cut(s) 52, 854, 1012, 2020, 2134, 3225, 3415
PstNI CAGNNNCTG 3 cut(s) 1622, 2024, 3139
PsuI RGATCY 4 cut(s) 1089, 1780, 1907, 3136
PvuII CAGCTG 3 cut(s) 2036, 2927, 3289
RseI CAYNNNNRTG 5 cut(s) 926, 976, 2366, 2403, 3798
SacI GAGCTC 1 cut(s) 2984
SacII CCGCGG 1 cut(s) 1096
Sau96I GGNCC 5 cut(s) 1528, 1757, 2605, 2750, 3490
ScaI AGTACT 1 cut(s) 2109
SchI GAGTC 2 cut(s) 2219, 3726
ScrFI CCNGG 7 cut(s) 119, 263, 1268, 1352, 2078, 3134, 3326
SduI GDGCHC 8 cut(s) 471, 1700, 1984, 2400, 2413, 2857, 2984, 3586
SfcI CTRYAG 9 cut(s) 48, 850, 1008, 2016, 2124, 2130, 3221, 3411, 3648
Sfr274I CTCGAG 1 cut(s) 2401
Sfr303I CCGCGG 1 cut(s) 1096
SgrBI CCGCGG 1 cut(s) 1096
SinI GGWCC 4 cut(s) 1757, 2605, 2750, 3490
SlaI CTCGAG 1 cut(s) 2401
SmiMI CAYNNNNRTG 5 cut(s) 926, 976, 2366, 2403, 3798
SmlI CTYRAG 3 cut(s) 1693, 1879, 2401
SmoI CTYRAG 3 cut(s) 1693, 1879, 2401
SphI GCATGC 2 cut(s) 2492, 2838
SseBI AGGCCT 1 cut(s) 1341
SsiI CCGC 7 cut(s) 879, 998, 1093, 1095, 1215, 2074, 3189
SspI AATATT 3 cut(s) 1155, 1390, 2997
SspMI CTAG 7 cut(s) 1182, 1487, 2117, 2178, 2576, 3033, 3566
SstI GAGCTC 1 cut(s) 2984
StuI AGGCCT 1 cut(s) 1341
StyD4I CCNGG 7 cut(s) 117, 261, 1266, 1350, 2076, 3132, 3324
StyI CCWWGG 1 cut(s) 555
TaiI ACGT 2 cut(s) 282, 405
TaqI TCGA 7 cut(s) 1175, 2042, 2402, 2601, 2857, 3628, 3643
TatI WGTACW 2 cut(s) 756, 2107
TauI GCSGC 2 cut(s) 882, 2076
TfiI GAWTC 6 cut(s) 23, 113, 1619, 2334, 3242, 3596
TseFI GTSAC 3 cut(s) 1946, 1955, 3248
Tsp45I GTSAC 3 cut(s) 1946, 1955, 3248
TspGWI ACGGA 1 cut(s) 1752
Van91I CCANNNNNTGG 1 cut(s) 561
VneI GTGCAC 1 cut(s) 2409
VpaK11BI GGWCC 4 cut(s) 1757, 2605, 2750, 3490
VspI ATTAAT 2 cut(s) 2243, 3681
XagI CCTNNNNNAGG 3 cut(s) 965, 2574, 3243
XapI RAATTY 5 cut(s) 1460, 1578, 2231, 3061, 3080
XceI RCATGY 3 cut(s) 2492, 2838, 3797
XcmI CCANNNNNNNNNTGG 2 cut(s) 2671, 3500
XhoI CTCGAG 1 cut(s) 2401
XspI CTAG 7 cut(s) 1182, 1487, 2117, 2178, 2576, 3033, 3566
ZrmI AGTACT 1 cut(s) 2109
Zsp2I ATGCAT 4 cut(s) 862, 2490, 2494, 2836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.