Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 286 |
| AccB1I | GGYRCC | 1 cut(s) | 286 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 26, 91 |
| AcuI | CTGAAG | 1 cut(s) | 311 |
| AfaI | GTAC | 1 cut(s) | 288 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 26, 91 |
| AgsI | TTSAA | 2 cut(s) | 236, 246 |
| AjnI | CCWGG | 1 cut(s) | 133 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 152, 184 |
| AluBI | AGCT | 1 cut(s) | 374 |
| AluI | AGCT | 1 cut(s) | 374 |
| ApeKI | GCWGC | 1 cut(s) | 72 |
| Asp718I | GGTACC | 1 cut(s) | 286 |
| AspS9I | GGNCC | 1 cut(s) | 270 |
| AvaII | GGWCC | 1 cut(s) | 270 |
| BanI | GGYRCC | 1 cut(s) | 286 |
| BbvI | GCAGC | 1 cut(s) | 84 |
| BccI | CCATC | 1 cut(s) | 178 |
| BciT130I | CCWGG | 1 cut(s) | 135 |
| BfaI | CTAG | 1 cut(s) | 381 |
| BisI | GCNGC | 1 cut(s) | 73 |
| BlsI | GCNGC | 1 cut(s) | 74 |
| Bme1390I | CCNGG | 1 cut(s) | 135 |
| Bme18I | GGWCC | 1 cut(s) | 270 |
| BmgT120I | GGNCC | 1 cut(s) | 270 |
| BmiI | GGNNCC | 2 cut(s) | 147, 288 |
| BmrFI | CCNGG | 1 cut(s) | 135 |
| BpmI | CTGGAG | 1 cut(s) | 238 |
| Bpu10I | CCTNAGC | 2 cut(s) | 282, 349 |
| BsaJI | CCNNGG | 1 cut(s) | 134 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 26, 91 |
| BseBI | CCWGG | 1 cut(s) | 135 |
| BseDI | CCNNGG | 1 cut(s) | 134 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 26, 91 |
| BseMII | CTCAG | 3 cut(s) | 282, 296, 363 |
| BseXI | GCAGC | 1 cut(s) | 84 |
| BsgI | GTGCAG | 1 cut(s) | 259 |
| BshNI | GGYRCC | 1 cut(s) | 286 |
| BsiSI | CCGG | 1 cut(s) | 69 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 26, 91 |
| BsmI | GAATGC | 1 cut(s) | 309 |
| Bsp143I | GATC | 1 cut(s) | 294 |
| BspCNI | CTCAG | 3 cut(s) | 283, 295, 362 |
| BspLI | GGNNCC | 2 cut(s) | 147, 288 |
| BspT107I | GGYRCC | 1 cut(s) | 286 |
| BssECI | CCNNGG | 1 cut(s) | 134 |
| BssMI | GATC | 1 cut(s) | 294 |
| Bst2UI | CCWGG | 1 cut(s) | 135 |
| Bst4CI | ACNGT | 1 cut(s) | 270 |
| Bst6I | CTCTTC | 1 cut(s) | 219 |
| BstDEI | CTNAG | 3 cut(s) | 282, 291, 349 |
| BstKTI | GATC | 1 cut(s) | 297 |
| BstMBI | GATC | 1 cut(s) | 294 |
| BstNI | CCWGG | 1 cut(s) | 135 |
| BstSCI | CCNGG | 1 cut(s) | 133 |
| BstV1I | GCAGC | 1 cut(s) | 84 |
| BtsI | GCAGTG | 3 cut(s) | 112, 276, 343 |
| BtsIMutI | CAGTG | 3 cut(s) | 112, 276, 343 |
| Cfr13I | GGNCC | 1 cut(s) | 270 |
| Csp6I | GTAC | 1 cut(s) | 287 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 120, 155 |
| CviJI | RGCY | 2 cut(s) | 122, 374 |
| CviKI_1 | RGCY | 2 cut(s) | 122, 374 |
| CviQI | GTAC | 1 cut(s) | 287 |
| DdeI | CTNAG | 3 cut(s) | 282, 291, 349 |
| DpnI | GATC | 1 cut(s) | 296 |
| DpnII | GATC | 1 cut(s) | 294 |
| Eam1104I | CTCTTC | 1 cut(s) | 219 |
| EarI | CTCTTC | 1 cut(s) | 219 |
| Eco47I | GGWCC | 1 cut(s) | 270 |
| Eco57I | CTGAAG | 1 cut(s) | 311 |
| EcoRII | CCWGG | 1 cut(s) | 133 |
| FaiI | YATR | 6 cut(s) | 82, 92, 113, 198, 222, 335 |
| Fnu4HI | GCNGC | 1 cut(s) | 73 |
| Fsp4HI | GCNGC | 1 cut(s) | 73 |
| FspBI | CTAG | 1 cut(s) | 381 |
| GluI | GCNGC | 1 cut(s) | 73 |
| GsuI | CTGGAG | 1 cut(s) | 238 |
| HapII | CCGG | 1 cut(s) | 69 |
| HinfI | GANTC | 1 cut(s) | 251 |
| HpaII | CCGG | 1 cut(s) | 69 |
| Hpy188III | TCNNGA | 1 cut(s) | 298 |
| HpyAV | CCTTC | 2 cut(s) | 169, 335 |
| HpyCH4III | ACNGT | 1 cut(s) | 270 |
| HpyCH4V | TGCA | 3 cut(s) | 142, 200, 276 |
| HpyF3I | CTNAG | 3 cut(s) | 282, 291, 349 |
| KpnI | GGTACC | 1 cut(s) | 290 |
| Kzo9I | GATC | 1 cut(s) | 294 |
| Lsp1109I | GCAGC | 1 cut(s) | 84 |
| MaeI | CTAG | 1 cut(s) | 381 |
| MaeIII | GTNAC | 2 cut(s) | 3, 341 |
| MalI | GATC | 1 cut(s) | 296 |
| MboI | GATC | 1 cut(s) | 294 |
| MboII | GAAGA | 2 cut(s) | 169, 236 |
| MfeI | CAATTG | 1 cut(s) | 246 |
| MluCI | AATT | 3 cut(s) | 231, 246, 309 |
| MlyI | GAGTC | 1 cut(s) | 245 |
| MmeI | TCCRAC | 1 cut(s) | 144 |
| MnlI | CCTC | 1 cut(s) | 307 |
| MseI | TTAA | 1 cut(s) | 216 |
| MspI | CCGG | 1 cut(s) | 69 |
| MspR9I | CCNGG | 1 cut(s) | 135 |
| MunI | CAATTG | 1 cut(s) | 246 |
| Mva1269I | GAATGC | 1 cut(s) | 309 |
| MvaI | CCWGG | 1 cut(s) | 135 |
| NdeII | GATC | 1 cut(s) | 294 |
| NlaIV | GGNNCC | 2 cut(s) | 147, 288 |
| NmuCI | GTSAC | 1 cut(s) | 341 |
| PctI | GAATGC | 1 cut(s) | 309 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 26, 91 |
| PkrI | GCNGC | 1 cut(s) | 74 |
| PleI | GAGTC | 1 cut(s) | 245 |
| PpsI | GAGTC | 1 cut(s) | 245 |
| Psp6I | CCWGG | 1 cut(s) | 133 |
| PspGI | CCWGG | 1 cut(s) | 133 |
| PspN4I | GGNNCC | 2 cut(s) | 147, 288 |
| PspPI | GGNCC | 1 cut(s) | 270 |
| RsaI | GTAC | 1 cut(s) | 288 |
| RsaNI | GTAC | 1 cut(s) | 287 |
| SaqAI | TTAA | 1 cut(s) | 216 |
| SatI | GCNGC | 1 cut(s) | 73 |
| Sau3AI | GATC | 1 cut(s) | 294 |
| Sau96I | GGNCC | 1 cut(s) | 270 |
| SchI | GAGTC | 1 cut(s) | 245 |
| ScrFI | CCNGG | 1 cut(s) | 135 |
| SetI | ASST | 5 cut(s) | 288, 292, 343, 355, 376 |
| SinI | GGWCC | 1 cut(s) | 270 |
| Sse9I | AATT | 3 cut(s) | 231, 246, 309 |
| SspMI | CTAG | 1 cut(s) | 381 |
| StyD4I | CCNGG | 1 cut(s) | 133 |
| TaaI | ACNGT | 1 cut(s) | 270 |
| TasI | AATT | 3 cut(s) | 231, 246, 309 |
| Tru1I | TTAA | 1 cut(s) | 216 |
| Tru9I | TTAA | 1 cut(s) | 216 |
| TscAI | CASTG | 3 cut(s) | 112, 283, 350 |
| TseFI | GTSAC | 1 cut(s) | 341 |
| TseI | GCWGC | 1 cut(s) | 72 |
| Tsp45I | GTSAC | 1 cut(s) | 341 |
| TspDTI | ATGAA | 2 cut(s) | 69, 237 |
| TspRI | CASTG | 3 cut(s) | 112, 283, 350 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 26, 91 |
| VpaK11BI | GGWCC | 1 cut(s) | 270 |
| XspI | CTAG | 1 cut(s) | 381 |