Prupe.5G121900_v2.0.a1

Beta-carotene isomerase D27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000150
Physical Location & Seq
Reverse (-)
12127770 .. 12130382
2613 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G121900.1

Sequence Viewer

Length: 912 bp
ATGGTGGTTCTAAGCCTTCAACCTGTCCAATTTTCTACCCCTAAACACATGTTATTATGTAGACCGGGCAGTACTGGTTATCCCATCCGATGTGGGATTGCAAAGCCATCAGCAGAGCCAGCTCCCGTGGGACAAGAGACCAAGTACAGTGAAAAAGTATATATCATGGAGCGTCGGAATAATGAATCCAAAGCTTCGGTGACCTCGGACCAGGTTTTGATGAGGATGGCTTCACGGCGGGAAGATGAGATCCATGACAAGTTATCGAAAATCCAGAAAGCTAGTACTAGTCGTCCAAATCCCACGGTGGTTGCTGGCAGGCCCACGGGGGAGCATGCATCAATGACGGTAAATCCGGGGAAAGCTAGGTCTATGTTTTTAGTGGTGTCTTTTTGTGAGGGCCGATACAAGGAGGCAATATATGAAGTAAAGTTCAAACGCAGATGTACAGGAGAAGTTTTTGATGATGATATGGGCCGAGAAGCAGCTCCACTTCTTGAACCTCTAGCCAAGTTCTCGAATATGTATTTCCTTCAGAGTGCGAGTATCTCCTCCAAAATATATCTACTTCAACAGGACACTCACGATGAAAGTTCTTGGCCCTCCCCCGCTAGATCATTGGGAGGATATGTTTTTGACACGGAGACTAGGGCAGTAATTCCATCGATTCCTCCTACCATAGCAACTAAACCAATGGCAAATGTTGTGTCTGCTAATGGGAAATTGTATTATCTTGCCTTTCCACACTGGTCCAGATATCCGGATGATCCATGCTTTGAGAGATATGATTCCAATCTGGAGGTTTGGGAGCCAATGCCTCCTTTTCCATCTTATGCCGTTTGTTATGGTGTTATTTTGTTTTCATTGTGCCAACATGTACAACTGGAATTCCAAAGTCCTTGCTTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

304

Amino Acids

34.08

Weight (kDa)

8.05

Isoelectric Point (pI)

49.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000150)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g02900 FvH4_5g02920 FvH4_5g15972 FvH4_5g25390 FvH4_7g13450 FvH4_7g13450 FvH4_7g27631 FvH4_7g27640 FvH4_7g27660 FvH4_7g27670 FvH4_7g27710 FvH4_7g27720
malus_domestica MD06G1078700.v1.1 MD06G1178900.v1.1 MD06G1179300.v1.1 MD06G1179400.v1.1 MD14G1191500.v1.1 MD14G1191600.v1.1 MD16G1080700.v1.1 MD17G1269500.v1.1
prunus_persica Prupe.1G178900_v2.0.a1 Prupe.2G232100_v2.0.a1 Prupe.3G199300_v2.0.a1 Prupe.3G199700_v2.0.a1 Prupe.3G199700_v2.0.a1 Prupe.3G199800_v2.0.a1 Prupe.5G121900_v2.0.a1 Prupe.5G149900_v2.0.a1 Prupe.5G149900_v2.0.a1 Prupe.5G150200_v2.0.a1 Prupe.5G179400_v2.0.a1 Prupe.5G179400_v2.0.a1 Prupe.5G190200_v2.0.a1 Prupe.5G190300_v2.0.a1 Prupe.5G190500_v2.0.a1 Prupe.5G190600_v2.0.a1 Prupe.5G190700_v2.0.a1 Prupe.5G190800_v2.0.a1 Prupe.5G196400_v2.0.a1 Prupe.5G196500_v2.0.a1 Prupe.5G196600_v2.0.a1 Prupe.5G196700_v2.0.a1 Prupe.5G196800_v2.0.a1 Prupe.5G216900_v2.0.a1 Prupe.5G216900_v2.0.a1 Prupe.5G216900_v2.0.a1 Prupe.7G215400_v2.0.a1 Prupe.7G215500_v2.0.a1 Prupe.7G215500_v2.0.a1 Prupe.8G014700_v2.0.a1 Prupe.8G014700_v2.0.a1 Prupe.I003600_v2.0.a1
pyrus_communis pycom06g15900 pycom06g15930
rosa_chinensis RchiOBHm_Chr1g0378721 RchiOBHm_Chr1g0379031 RchiOBHm_Chr1g0379041 RchiOBHm_Chr1g0379111 RchiOBHm_Chr1g0379231 RchiOBHm_Chr1g0379381 RchiOBHm_Chr7g0205711 RchiOBHm_Chr7g0219841
rosa_laevigata RLG00000002638 RLG00000002641 RLG00000003360 RLG00000003362 RLG00000003364 RLG00000003972 RLG00000003975 RLG00000003976 RLG00000005302 RLG00000005303 RLG00000026142 RLG00000026318 RLG00000026378 RLG00000026379 RLG00000026396 RLG00000026404 RLG00000026405 RLG00000026412 RLG00000026413 RLG00000026766 RLG00000026768 RLG00000026774 RLG00000026775 RLG00000026776 RLG00000026778 RLG00000026779 RLG00000033034
rosa_multiflora Rmu_co8058818.1_g000001 Rmu_co8428857.1_g000001 Rmu_sc0000556.1_g000004 Rmu_sc0000556.1_g000017 Rmu_sc0000556.1_g000023 Rmu_sc0000556.1_g000038 Rmu_sc0000556.1_g000044 Rmu_sc0000556.1_g000046 Rmu_sc0000634.1_g000002 Rmu_sc0001232.1_g000027 Rmu_sc0003964.1_g000004 Rmu_sc0003964.1_g000013 Rmu_sc0006124.1_g000015 Rmu_sc0006761.1_g000020 Rmu_sc0006761.1_g000023 Rmu_sc0015070.1_g000001 Rmu_sc0017925.1_g000001 Rmu_sc0031430.1_g000005 Rmu_ssc0000119.1_g000014
rosa_roxburghii Rroxscaffold_2G00080740 Rroxscaffold_2G00090730 Rroxscaffold_3G00227790 Rroxscaffold_3G00235420 Rroxscaffold_3G00243990 Rroxscaffold_3G00244010 Rroxscaffold_3G00252040 Rroxscaffold_3G00252060 Rroxscaffold_3G00258760 Rroxscaffold_3G00258780 Rroxscaffold_3G00258790 Rroxscaffold_4G00280120 Rroxscaffold_4G00280210 Rroxscaffold_4G00280240 Rroxscaffold_4G00280300 Rroxscaffold_4G00280310 Rroxscaffold_5G00348380 Rroxscaffold_5G00352440 Rroxscaffold_5G00381760 Rroxscaffold_5G00381770 Rroxscaffold_7G00205800 Rroxscaffold_7G00205810 Rroxscaffold_7G00205850
rosa_rugosa Rorug01G0410800.1 Rorug01G0411700 Rorug01G0412500 Rorug06G0429700 Rorug07G0155400 Rorug07G0155600
rosa_samantha Rh1AG394500 Rh1AG433000 Rh1AG433600 Rh1AG434900 Rh1AG435200 Rh1BG359100 Rh1BG391200 Rh1BG392000 Rh1BG392700 Rh1BG399000 Rh1BG420100 Rh1BG420200 Rh1CG372200 Rh1CG403900 Rh1CG404800 Rh1CG412400 Rh1DG389700 Rh1DG389800 Rh1DG422400 Rh1DG423000 Rh1DG423500 Rh1DG452800 Rh1DG452900 Rh2AG541500 Rh2AG627500 Rh2BG637600 Rh2BG637700 Rh2CG524200 Rh2DG563600 Rh2DG563700 Rh2DG649200 Rh5CG213000 Rh6DG081900 Rh7AG030200 Rh7AG223700 Rh7AG224100 Rh7BG029600 Rh7BG030900 Rh7BG218800 Rh7BG283400 Rh7BG283700 Rh7BG283900 Rh7BG284100 Rh7BG284500 Rh7CG031200 Rh7CG031300 Rh7CG237000 Rh7CG237600 Rh7CG312000 Rh7DG030500 Rh7DG230800
rosa_wichuraiana Rw1G037920 Rw1G037950 Rw1G040890 Rw7G019300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 61
AccIII TCCGGA 1 cut(s) 760
AciI CCGC 2 cut(s) 238, 609
AclWI GGATC 2 cut(s) 244, 761
AcsI RAATTY 1 cut(s) 887
AcuI CTGAAG 1 cut(s) 518
AfaI GTAC 5 cut(s) 73, 146, 286, 448, 879
AfiI CCNNNNNNNGG 1 cut(s) 409
AflIII ACRYGT 2 cut(s) 48, 874
AgsI TTSAA 4 cut(s) 20, 436, 500, 572
AhlI ACTAGT 1 cut(s) 287
AjnI CCWGG 1 cut(s) 210
AluBI AGCT 5 cut(s) 122, 194, 281, 365, 488
AluI AGCT 5 cut(s) 122, 194, 281, 365, 488
Alw26I GTCTC 2 cut(s) 131, 638
AlwI GGATC 2 cut(s) 244, 761
Aor13HI TCCGGA 1 cut(s) 760
AoxI GGCC 4 cut(s) 320, 400, 475, 599
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 1 cut(s) 887
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
AspS9I GGNCC 6 cut(s) 208, 321, 400, 475, 600, 750
AsuC2I CCSGG 2 cut(s) 66, 357
AsuHPI GGTGA 1 cut(s) 211
AvaII GGWCC 2 cut(s) 208, 750
BbvI GCAGC 1 cut(s) 497
BccI CCATC 5 cut(s) 92, 115, 220, 670, 835
BceAI ACGGC 2 cut(s) 251, 821
BciT130I CCWGG 1 cut(s) 212
BcnI CCSGG 2 cut(s) 66, 357
BcoDI GTCTC 2 cut(s) 131, 638
BcuI ACTAGT 1 cut(s) 287
BfaI CTAG 6 cut(s) 282, 288, 366, 506, 612, 648
BisI GCNGC 1 cut(s) 486
BlsI GCNGC 1 cut(s) 487
BmcAI AGTACT 2 cut(s) 73, 286
Bme1390I CCNGG 3 cut(s) 66, 212, 357
Bme18I GGWCC 2 cut(s) 208, 750
BmgT120I GGNCC 6 cut(s) 208, 321, 400, 475, 600, 750
BmiI GGNNCC 1 cut(s) 810
BmrFI CCNGG 3 cut(s) 66, 212, 357
BmsI GCATC 1 cut(s) 347
BpmI CTGGAG 1 cut(s) 818
BpuMI CCSGG 2 cut(s) 66, 357
Bsa29I ATCGAT 1 cut(s) 665
BsaBI GATNNNNATC 1 cut(s) 792
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 5 cut(s) 126, 204, 303, 324, 356
BsaWI WCCGGW 1 cut(s) 760
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bsc4I CCNNNNNNNGG 1 cut(s) 409
Bse1I ACTGG 3 cut(s) 79, 752, 888
Bse8I GATNNNNATC 1 cut(s) 792
BseAI TCCGGA 1 cut(s) 760
BseBI CCWGG 1 cut(s) 212
BseCI ATCGAT 1 cut(s) 665
BseDI CCNNGG 5 cut(s) 126, 204, 303, 324, 356
BseGI GGATG 3 cut(s) 84, 231, 769
BseJI GATNNNNATC 1 cut(s) 792
BseLI CCNNNNNNNGG 1 cut(s) 409
BseNI ACTGG 3 cut(s) 79, 752, 888
BseRI GAGGAG 1 cut(s) 541
BseXI GCAGC 1 cut(s) 497
BshFI GGCC 4 cut(s) 322, 402, 477, 601
BshVI ATCGAT 1 cut(s) 665
BsiSI CCGG 3 cut(s) 65, 356, 761
BslFI GGGAC 1 cut(s) 144
BslI CCNNNNNNNGG 1 cut(s) 409
BsmAI GTCTC 2 cut(s) 131, 638
BsmFI GGGAC 1 cut(s) 144
BsnI GGCC 4 cut(s) 322, 402, 477, 601
Bso31I GGTCTC 1 cut(s) 131
Bsp13I TCCGGA 1 cut(s) 760
Bsp1407I TGTACA 2 cut(s) 446, 877
Bsp143I GATC 3 cut(s) 249, 614, 766
BspACI CCGC 2 cut(s) 238, 609
BspANI GGCC 4 cut(s) 322, 402, 477, 601
BspDI ATCGAT 1 cut(s) 665
BspEI TCCGGA 1 cut(s) 760
BspHI TCATGA 1 cut(s) 908
BspLI GGNNCC 1 cut(s) 810
BspPI GGATC 2 cut(s) 244, 761
BspTNI GGTCTC 1 cut(s) 131
BsrGI TGTACA 2 cut(s) 446, 877
BsrI ACTGG 3 cut(s) 79, 752, 888
BssECI CCNNGG 5 cut(s) 126, 204, 303, 324, 356
BssMI GATC 3 cut(s) 249, 614, 766
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 3 cut(s) 149, 307, 349
BstAUI TGTACA 2 cut(s) 446, 877
BstC8I GCNNGC 4 cut(s) 120, 316, 320, 336
BstDEI CTNAG 1 cut(s) 11
BstDSI CCRYGG 3 cut(s) 126, 303, 324
BstEII GGTNACC 1 cut(s) 199
BstF5I GGATG 3 cut(s) 84, 231, 769
BstKTI GATC 3 cut(s) 252, 617, 769
BstMAI GTCTC 2 cut(s) 131, 638
BstMBI GATC 3 cut(s) 249, 614, 766
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 3 cut(s) 52, 338, 878
BstPI GGTNACC 1 cut(s) 199
BstSCI CCNGG 3 cut(s) 64, 210, 355
BstV1I GCAGC 1 cut(s) 497
BstX2I RGATCY 1 cut(s) 249
BstYI RGATCY 1 cut(s) 249
Bsu15I ATCGAT 1 cut(s) 665
BsuRI GGCC 4 cut(s) 322, 402, 477, 601
BsuTUI ATCGAT 1 cut(s) 665
BtgI CCRYGG 3 cut(s) 126, 303, 324
BtsCI GGATG 3 cut(s) 84, 231, 769
BtsIMutI CAGTG 2 cut(s) 154, 745
Cac8I GCNNGC 4 cut(s) 120, 316, 320, 336
CciI TCATGA 1 cut(s) 908
Cfr13I GGNCC 6 cut(s) 208, 321, 400, 475, 600, 750
ClaI ATCGAT 1 cut(s) 665
CseI GACGC 1 cut(s) 161
CsiI ACCWGGT 1 cut(s) 210
Csp6I GTAC 5 cut(s) 72, 145, 285, 447, 878
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviAII CATG 7 cut(s) 49, 166, 254, 335, 771, 875, 909
CviQI GTAC 5 cut(s) 72, 145, 285, 447, 878
DdeI CTNAG 1 cut(s) 11
DpnI GATC 3 cut(s) 251, 616, 768
DpnII GATC 3 cut(s) 249, 614, 766
Eco31I GGTCTC 1 cut(s) 131
Eco32I GATATC 1 cut(s) 758
Eco47I GGWCC 2 cut(s) 208, 750
Eco57I CTGAAG 1 cut(s) 518
Eco91I GGTNACC 1 cut(s) 199
EcoO65I GGTNACC 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 887
EcoRII CCWGG 1 cut(s) 210
EcoRV GATATC 1 cut(s) 758
EcoT22I ATGCAT 1 cut(s) 340
FaeI CATG 7 cut(s) 52, 169, 257, 338, 774, 878, 912
FaqI GGGAC 1 cut(s) 144
FatI CATG 7 cut(s) 48, 165, 253, 334, 770, 874, 908
FauI CCCGC 2 cut(s) 231, 616
FblI GTMKAC 1 cut(s) 61
Fnu4HI GCNGC 1 cut(s) 486
FokI GGATG 3 cut(s) 71, 238, 776
Fsp4HI GCNGC 1 cut(s) 486
FspBI CTAG 6 cut(s) 282, 288, 366, 506, 612, 648
GluI GCNGC 1 cut(s) 486
GsuI CTGGAG 1 cut(s) 818
HaeIII GGCC 4 cut(s) 322, 402, 477, 601
HapII CCGG 3 cut(s) 65, 356, 761
HgaI GACGC 1 cut(s) 161
Hin1II CATG 7 cut(s) 52, 169, 257, 338, 774, 878, 912
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 3 cut(s) 185, 667, 788
HpaII CCGG 3 cut(s) 65, 356, 761
HphI GGTGA 1 cut(s) 211
Hpy166II GTNNAC 1 cut(s) 62
Hpy188I TCNGA 4 cut(s) 89, 177, 208, 537
Hpy188III TCNNGA 8 cut(s) 274, 497, 517, 584, 753, 761, 797, 909
Hpy8I GTNNAC 1 cut(s) 62
Hpy99I CGWCG 1 cut(s) 177
HpyAV CCTTC 2 cut(s) 26, 542
HpyCH4III ACNGT 3 cut(s) 149, 307, 349
HpyCH4V TGCA 2 cut(s) 101, 338
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 1 cut(s) 11
Hsp92II CATG 7 cut(s) 52, 169, 257, 338, 774, 878, 912
Kpn2I TCCGGA 1 cut(s) 760
Kzo9I GATC 3 cut(s) 249, 614, 766
LmnI GCTCC 5 cut(s) 127, 169, 331, 493, 808
Lsp1109I GCAGC 1 cut(s) 497
LweI GCATC 1 cut(s) 347
MabI ACCWGGT 1 cut(s) 210
MaeI CTAG 6 cut(s) 282, 288, 366, 506, 612, 648
MaeIII GTNAC 1 cut(s) 199
MalI GATC 3 cut(s) 251, 616, 768
MboI GATC 3 cut(s) 249, 614, 766
MboII GAAGA 1 cut(s) 254
MflI RGATCY 1 cut(s) 249
MluCI AATT 4 cut(s) 29, 657, 722, 887
MmeI TCCRAC 1 cut(s) 155
Mph1103I ATGCAT 1 cut(s) 340
MroI TCCGGA 1 cut(s) 760
MspI CCGG 3 cut(s) 65, 356, 761
MspR9I CCNGG 3 cut(s) 66, 212, 357
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 119
NciI CCSGG 2 cut(s) 66, 357
NdeII GATC 3 cut(s) 249, 614, 766
NlaIII CATG 7 cut(s) 52, 169, 257, 338, 774, 878, 912
NlaIV GGNNCC 1 cut(s) 810
NmeAIII GCCGAG 1 cut(s) 503
NmuCI GTSAC 1 cut(s) 199
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 3 cut(s) 52, 338, 878
PaeI GCATGC 1 cut(s) 338
PagI TCATGA 1 cut(s) 908
PciI ACATGT 2 cut(s) 48, 874
PfeI GAWTC 3 cut(s) 185, 667, 788
PkrI GCNGC 1 cut(s) 487
PscI ACATGT 2 cut(s) 48, 874
Psp6I CCWGG 1 cut(s) 210
PspEI GGTNACC 1 cut(s) 199
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 1 cut(s) 810
PspPI GGNCC 6 cut(s) 208, 321, 400, 475, 600, 750
PsuI RGATCY 1 cut(s) 249
RsaI GTAC 5 cut(s) 73, 146, 286, 448, 879
RsaNI GTAC 5 cut(s) 72, 145, 285, 447, 878
SatI GCNGC 1 cut(s) 486
Sau3AI GATC 3 cut(s) 249, 614, 766
Sau96I GGNCC 6 cut(s) 208, 321, 400, 475, 600, 750
ScaI AGTACT 2 cut(s) 73, 286
ScrFI CCNGG 3 cut(s) 66, 212, 357
SexAI ACCWGGT 1 cut(s) 210
SfaNI GCATC 1 cut(s) 347
SinI GGWCC 2 cut(s) 208, 750
SpeI ACTAGT 1 cut(s) 287
SphI GCATGC 1 cut(s) 338
Sse9I AATT 4 cut(s) 29, 657, 722, 887
SsiI CCGC 2 cut(s) 238, 609
SspMI CTAG 6 cut(s) 282, 288, 366, 506, 612, 648
StyD4I CCNGG 3 cut(s) 64, 210, 355
TaaI ACNGT 3 cut(s) 149, 307, 349
TaqI TCGA 3 cut(s) 266, 518, 665
TasI AATT 4 cut(s) 29, 657, 722, 887
TatI WGTACW 5 cut(s) 71, 144, 284, 446, 877
TfiI GAWTC 3 cut(s) 185, 667, 788
TscAI CASTG 2 cut(s) 154, 752
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 5 cut(s) 198, 438, 603, 852, 897
TspGWI ACGGA 1 cut(s) 656
TspRI CASTG 2 cut(s) 154, 752
VpaK11BI GGWCC 2 cut(s) 208, 750
XapI RAATTY 1 cut(s) 887
XceI RCATGY 3 cut(s) 52, 338, 878
XmiI GTMKAC 1 cut(s) 61
XspI CTAG 6 cut(s) 282, 288, 366, 506, 612, 648
ZrmI AGTACT 2 cut(s) 73, 286
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.