Rroxscaffold_3G00258760

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000150
Physical Location & Seq
Reverse (-)
53222476 .. 53224535
2060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00258760.1

Sequence Viewer

Length: 1341 bp
ATGCCAGACCCCAGCGAGCTCGATCTGGGTCGCCATGACTTGAGTTGTAACCCGATGGACAAGTGGGAATCTCGAACTGTTTATTTTCTGGTTCACTTTATTGGCGGCGGGCCGGGACATGCCCTATATGAAGTAAAGATTGAGGGAGGAAAAATCATGGGAAAAAGAGGTGGATTACTTGAGCCTGTATGCAAGTTTTTTGATAGGACTAATCCTGAGGTTCCCATGGAATATCTCTTGGGCGCTACCAGGTTTGGCAGCCGCTCCAAATTGTATTTATTGCAACACGAAGGGTTTGGCAGCTGGTGGCTCAGGGAGAGCAGCACCCGCAGCCGTGACGCATATAAAGGATGCGTATTTGACACTATGACCAGGAAATTAATGCCATTCAAGGTGCCTACAGTTAAGAAAGATGCCCTTTTGATTTCTGCTTATGGCAAGCTTTATCATATTGCACATCCAGCATCATTTATGCCAGACCCCTCTTTCGAGGTTTATGATCCACACCCTGGCTCCTGGACAAGACTCTCTACATTCCCTTCCTATCGTATTTCAACTCCCTGTCGCATAGTTGGTTATGCTGTTTGTTACGACTGTATTTTGATGTCTATAACGGGCCATGGGACTGGTAACTTCTGGGTATATCAGGTGACTTTGGATCAATGGAGACAGGTCAATATCCCTACCGACAGATATTCAATTCATCATTATCCTTTCGAAGGGAGAGCTGTGGTTGTAGGCGATACTATTTACGCCTTATCTGCTCTTGGGAGTATTATGGCATTCTCTCTTATAATGGAAGAATTGGCTGACGGTACTATTAACTACTCTCTCGACAAACCAGTCTTGTTGTCTGGCTTGCGTACTTTATTTCTCAGGGATTCCGAATGTTCACCAAGTACAGACTATTTGGTTCATTTGGGGGGCTTGAACTTCTGCCTTTTGCAAACTGTCAGGAGAGATGTAACTGTGCAACGTGTGTGGATTACCACTTTTGATATTGTTTCCTGTGGAGGAAGAAGGCGTATCCGTACTCTAGCTACTACTATGCGTAAAGTGTCTATTAAGGGTCTCGGTCGATTCCTTCTTGACTCAGGATTCTCTCTAGAATGTGAAGACTTAGAACCCAAAGGGAAAGAGACTATGACTAACACCAGCATGATCCAGCCAACTGATGCTCCTCGACTGCACAAGCATGAAAAGGCTACACTCAAAATTATAAAGAGGCAGATTCACAAACAGAAACGAAAGATGATGAAGCCTAGATCAAAGAGGTTGGCCATGATGCAAAAGCTGTTCAGACCCCGTCGACATCGTAAGGAGGTCAAATGTGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

446

Amino Acids

50.96

Weight (kDa)

9.52

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000150)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g02900 FvH4_5g02920 FvH4_5g15972 FvH4_5g25390 FvH4_7g13450 FvH4_7g13450 FvH4_7g27631 FvH4_7g27640 FvH4_7g27660 FvH4_7g27670 FvH4_7g27710 FvH4_7g27720
malus_domestica MD06G1078700.v1.1 MD06G1178900.v1.1 MD06G1179300.v1.1 MD06G1179400.v1.1 MD14G1191500.v1.1 MD14G1191600.v1.1 MD16G1080700.v1.1 MD17G1269500.v1.1
prunus_persica Prupe.1G178900_v2.0.a1 Prupe.2G232100_v2.0.a1 Prupe.3G199300_v2.0.a1 Prupe.3G199700_v2.0.a1 Prupe.3G199700_v2.0.a1 Prupe.3G199800_v2.0.a1 Prupe.5G121900_v2.0.a1 Prupe.5G149900_v2.0.a1 Prupe.5G149900_v2.0.a1 Prupe.5G150200_v2.0.a1 Prupe.5G179400_v2.0.a1 Prupe.5G179400_v2.0.a1 Prupe.5G190200_v2.0.a1 Prupe.5G190300_v2.0.a1 Prupe.5G190500_v2.0.a1 Prupe.5G190600_v2.0.a1 Prupe.5G190700_v2.0.a1 Prupe.5G190800_v2.0.a1 Prupe.5G196400_v2.0.a1 Prupe.5G196500_v2.0.a1 Prupe.5G196600_v2.0.a1 Prupe.5G196700_v2.0.a1 Prupe.5G196800_v2.0.a1 Prupe.5G216900_v2.0.a1 Prupe.5G216900_v2.0.a1 Prupe.5G216900_v2.0.a1 Prupe.7G215400_v2.0.a1 Prupe.7G215500_v2.0.a1 Prupe.7G215500_v2.0.a1 Prupe.8G014700_v2.0.a1 Prupe.8G014700_v2.0.a1 Prupe.I003600_v2.0.a1
pyrus_communis pycom06g15900 pycom06g15930
rosa_chinensis RchiOBHm_Chr1g0378721 RchiOBHm_Chr1g0379031 RchiOBHm_Chr1g0379041 RchiOBHm_Chr1g0379111 RchiOBHm_Chr1g0379231 RchiOBHm_Chr1g0379381 RchiOBHm_Chr7g0205711 RchiOBHm_Chr7g0219841
rosa_laevigata RLG00000002638 RLG00000002641 RLG00000003360 RLG00000003362 RLG00000003364 RLG00000003972 RLG00000003975 RLG00000003976 RLG00000005302 RLG00000005303 RLG00000026142 RLG00000026318 RLG00000026378 RLG00000026379 RLG00000026396 RLG00000026404 RLG00000026405 RLG00000026412 RLG00000026413 RLG00000026766 RLG00000026768 RLG00000026774 RLG00000026775 RLG00000026776 RLG00000026778 RLG00000026779 RLG00000033034
rosa_multiflora Rmu_co8058818.1_g000001 Rmu_co8428857.1_g000001 Rmu_sc0000556.1_g000004 Rmu_sc0000556.1_g000017 Rmu_sc0000556.1_g000023 Rmu_sc0000556.1_g000038 Rmu_sc0000556.1_g000044 Rmu_sc0000556.1_g000046 Rmu_sc0000634.1_g000002 Rmu_sc0001232.1_g000027 Rmu_sc0003964.1_g000004 Rmu_sc0003964.1_g000013 Rmu_sc0006124.1_g000015 Rmu_sc0006761.1_g000020 Rmu_sc0006761.1_g000023 Rmu_sc0015070.1_g000001 Rmu_sc0017925.1_g000001 Rmu_sc0031430.1_g000005 Rmu_ssc0000119.1_g000014
rosa_roxburghii Rroxscaffold_2G00080740 Rroxscaffold_2G00090730 Rroxscaffold_3G00227790 Rroxscaffold_3G00235420 Rroxscaffold_3G00243990 Rroxscaffold_3G00244010 Rroxscaffold_3G00252040 Rroxscaffold_3G00252060 Rroxscaffold_3G00258760 Rroxscaffold_3G00258780 Rroxscaffold_3G00258790 Rroxscaffold_4G00280120 Rroxscaffold_4G00280210 Rroxscaffold_4G00280240 Rroxscaffold_4G00280300 Rroxscaffold_4G00280310 Rroxscaffold_5G00348380 Rroxscaffold_5G00352440 Rroxscaffold_5G00381760 Rroxscaffold_5G00381770 Rroxscaffold_7G00205800 Rroxscaffold_7G00205810 Rroxscaffold_7G00205850
rosa_rugosa Rorug01G0410800.1 Rorug01G0411700 Rorug01G0412500 Rorug06G0429700 Rorug07G0155400 Rorug07G0155600
rosa_samantha Rh1AG394500 Rh1AG433000 Rh1AG433600 Rh1AG434900 Rh1AG435200 Rh1BG359100 Rh1BG391200 Rh1BG392000 Rh1BG392700 Rh1BG399000 Rh1BG420100 Rh1BG420200 Rh1CG372200 Rh1CG403900 Rh1CG404800 Rh1CG412400 Rh1DG389700 Rh1DG389800 Rh1DG422400 Rh1DG423000 Rh1DG423500 Rh1DG452800 Rh1DG452900 Rh2AG541500 Rh2AG627500 Rh2BG637600 Rh2BG637700 Rh2CG524200 Rh2DG563600 Rh2DG563700 Rh2DG649200 Rh5CG213000 Rh6DG081900 Rh7AG030200 Rh7AG223700 Rh7AG224100 Rh7BG029600 Rh7BG030900 Rh7BG218800 Rh7BG283400 Rh7BG283700 Rh7BG283900 Rh7BG284100 Rh7BG284500 Rh7CG031200 Rh7CG031300 Rh7CG237000 Rh7CG237600 Rh7CG312000 Rh7DG030500 Rh7DG230800
rosa_wichuraiana Rw1G037920 Rw1G037950 Rw1G040890 Rw7G019300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 794, 1220
AasI GACNNNNNNGTC 1 cut(s) 842
AccB1I GGYRCC 1 cut(s) 394
AccB7I CCANNNNNTGG 1 cut(s) 509
AccBSI CCGCTC 1 cut(s) 264
AccI GTMKAC 1 cut(s) 1309
AciI CCGC 4 cut(s) 105, 108, 262, 328
AclWI GGATC 3 cut(s) 494, 666, 1156
AcoI YGGCCR 1 cut(s) 1278
AfaI GTAC 4 cut(s) 817, 865, 901, 1033
AfiI CCNNNNNNNGG 2 cut(s) 509, 719
AflIII ACRYGT 1 cut(s) 976
AgsI TTSAA 4 cut(s) 391, 555, 699, 931
AjnI CCWGG 4 cut(s) 248, 371, 508, 515
AluBI AGCT 6 cut(s) 19, 303, 442, 728, 1040, 1294
AluI AGCT 6 cut(s) 19, 303, 442, 728, 1040, 1294
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 3 cut(s) 661, 1076, 1133
AlwI GGATC 3 cut(s) 494, 666, 1156
AoxI GGCC 3 cut(s) 110, 616, 1278
ApeKI GCWGC 4 cut(s) 258, 300, 321, 330
ArsI GACNNNNNNTTYG 2 cut(s) 470, 502
AseI ATTAAT 1 cut(s) 380
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 2 cut(s) 110, 616
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 2 cut(s) 661, 885
AsuII TTCGAA 1 cut(s) 717
AxyI CCTNAGG 1 cut(s) 216
BalI TGGCCA 1 cut(s) 1280
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 1 cut(s) 21
BbsI GAAGAC 1 cut(s) 1122
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 4 cut(s) 270, 312, 333, 342
BccI CCATC 1 cut(s) 49
BceAI ACGGC 1 cut(s) 318
BciT130I CCWGG 4 cut(s) 250, 373, 510, 517
BciVI GTATCC 1 cut(s) 1037
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 3 cut(s) 661, 1076, 1133
BfaI CTAG 3 cut(s) 1037, 1106, 1263
BfmI CTRYAG 1 cut(s) 399
BfoI RGCGCY 1 cut(s) 246
BfuI GTATCC 1 cut(s) 1037
BisI GCNGC 6 cut(s) 106, 259, 262, 301, 322, 331
BlsI GCNGC 6 cut(s) 107, 260, 263, 302, 323, 332
Bme1390I CCNGG 5 cut(s) 114, 250, 373, 510, 517
BmgT120I GGNCC 2 cut(s) 110, 616
BmiI GGNNCC 3 cut(s) 222, 396, 514
BmrFI CCNGG 5 cut(s) 114, 250, 373, 510, 517
BmsI GCATC 6 cut(s) 341, 403, 473, 1165, 1275, 1324
BpiI GAAGAC 1 cut(s) 1122
Bpu10I CCTNAGC 1 cut(s) 311
Bpu14I TTCGAA 1 cut(s) 717
BpuEI CTTGAG 2 cut(s) 61, 200
BpuMI CCSGG 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 1076
BsaJI CCNNGG 3 cut(s) 225, 508, 619
BsaXI ACNNNNNCTCC 4 cut(s) 497, 527, 1162, 1192
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 719
Bse1I ACTGG 2 cut(s) 631, 842
Bse21I CCTNAGG 1 cut(s) 216
BseBI CCWGG 4 cut(s) 250, 373, 510, 517
BseDI CCNNGG 3 cut(s) 225, 508, 619
BseGI GGATG 2 cut(s) 356, 457
BseLI CCNNNNNNNGG 2 cut(s) 509, 719
BseMII CTCAG 4 cut(s) 207, 325, 889, 1107
BseNI ACTGG 2 cut(s) 631, 842
BseRI GAGGAG 1 cut(s) 1170
BseXI GCAGC 4 cut(s) 270, 312, 333, 342
BseYI CCCAGC 1 cut(s) 11
BsgI GTGCAG 1 cut(s) 1172
Bsh1285I CGRYCG 1 cut(s) 1078
BshFI GGCC 3 cut(s) 112, 618, 1280
BshNI GGYRCC 1 cut(s) 394
BsiEI CGRYCG 1 cut(s) 1078
BsiHKAI GWGCWC 1 cut(s) 21
BsiSI CCGG 1 cut(s) 113
BslFI GGGAC 2 cut(s) 129, 637
BslI CCNNNNNNNGG 2 cut(s) 509, 719
BsmAI GTCTC 3 cut(s) 661, 1076, 1133
BsmFI GGGAC 2 cut(s) 129, 637
BsmI GAATGC 1 cut(s) 782
BsnI GGCC 3 cut(s) 112, 618, 1280
Bso31I GGTCTC 1 cut(s) 1076
Bsp119I TTCGAA 1 cut(s) 717
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 5 cut(s) 22, 499, 658, 1161, 1265
Bsp19I CCATGG 2 cut(s) 225, 619
BspACI CCGC 4 cut(s) 105, 108, 262, 328
BspANI GGCC 3 cut(s) 112, 618, 1280
BspCNI CTCAG 4 cut(s) 208, 324, 888, 1106
BspLI GGNNCC 3 cut(s) 222, 396, 514
BspPI GGATC 3 cut(s) 494, 666, 1156
BspT104I TTCGAA 1 cut(s) 717
BspT107I GGYRCC 1 cut(s) 394
BspTNI GGTCTC 1 cut(s) 1076
BsrBI CCGCTC 1 cut(s) 264
BsrI ACTGG 2 cut(s) 631, 842
BssECI CCNNGG 3 cut(s) 225, 508, 619
BssMI GATC 5 cut(s) 22, 499, 658, 1161, 1265
BssT1I CCWWGG 2 cut(s) 225, 619
Bst2UI CCWGG 4 cut(s) 250, 373, 510, 517
Bst4CI ACNGT 6 cut(s) 79, 403, 596, 815, 952, 970
BstBI TTCGAA 1 cut(s) 717
BstC8I GCNNGC 4 cut(s) 17, 110, 440, 860
BstDEI CTNAG 5 cut(s) 216, 311, 875, 1093, 1120
BstDSI CCRYGG 2 cut(s) 225, 619
BstENI CCTNNNNNAGG 1 cut(s) 717
BstF5I GGATG 2 cut(s) 356, 457
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 5 cut(s) 25, 502, 661, 1164, 1268
BstMAI GTCTC 3 cut(s) 661, 1076, 1133
BstMBI GATC 5 cut(s) 22, 499, 658, 1161, 1265
BstMCI CGRYCG 1 cut(s) 1078
BstMWI GCNNNNNNNGC 4 cut(s) 327, 330, 461, 761
BstNI CCWGG 4 cut(s) 250, 373, 510, 517
BstNSI RCATGY 1 cut(s) 122
BstSCI CCNGG 5 cut(s) 112, 248, 371, 508, 515
BstSFI CTRYAG 1 cut(s) 399
BstV1I GCAGC 4 cut(s) 270, 312, 333, 342
BstV2I GAAGAC 1 cut(s) 1122
BstXI CCANNNNNNTGG 1 cut(s) 626
Bsu36I CCTNAGG 1 cut(s) 216
BsuI GTATCC 1 cut(s) 1037
BsuRI GGCC 3 cut(s) 112, 618, 1280
BtgI CCRYGG 2 cut(s) 225, 619
BtsCI GGATG 2 cut(s) 356, 457
Cac8I GCNNGC 4 cut(s) 17, 110, 440, 860
CfoI GCGC 1 cut(s) 245
Cfr13I GGNCC 2 cut(s) 110, 616
CseI GACGC 1 cut(s) 347
CsiI ACCWGGT 1 cut(s) 248
Csp6I GTAC 4 cut(s) 816, 864, 900, 1032
CspCI CAANNNNNGTGG 2 cut(s) 962, 997
CviAII CATG 8 cut(s) 35, 119, 157, 226, 620, 1159, 1196, 1282
CviQI GTAC 4 cut(s) 816, 864, 900, 1032
DdeI CTNAG 5 cut(s) 216, 311, 875, 1093, 1120
DpnI GATC 5 cut(s) 24, 501, 660, 1163, 1267
DpnII GATC 5 cut(s) 22, 499, 658, 1161, 1265
DrdI GACNNNNNNGTC 1 cut(s) 842
DseDI GACNNNNNNGTC 1 cut(s) 842
EaeI YGGCCR 1 cut(s) 1278
Ecl136II GAGCTC 1 cut(s) 19
Eco130I CCWWGG 2 cut(s) 225, 619
Eco24I GRGCYC 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 1076
Eco53kI GAGCTC 1 cut(s) 19
Eco81I CCTNAGG 1 cut(s) 216
EcoICRI GAGCTC 1 cut(s) 19
EcoNI CCTNNNNNAGG 1 cut(s) 717
EcoRII CCWGG 4 cut(s) 248, 371, 508, 515
EcoT14I CCWWGG 2 cut(s) 225, 619
EcoT38I GRGCYC 1 cut(s) 21
ErhI CCWWGG 2 cut(s) 225, 619
FaeI CATG 8 cut(s) 38, 122, 160, 229, 623, 1162, 1199, 1285
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FaqI GGGAC 2 cut(s) 129, 637
FatI CATG 8 cut(s) 34, 118, 156, 225, 619, 1158, 1195, 1281
FauI CCCGC 2 cut(s) 101, 335
FblI GTMKAC 1 cut(s) 1309
Fnu4HI GCNGC 6 cut(s) 106, 259, 262, 301, 322, 331
FokI GGATG 2 cut(s) 363, 444
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 6 cut(s) 106, 259, 262, 301, 322, 331
FspBI CTAG 3 cut(s) 1037, 1106, 1263
GlaI GCGC 1 cut(s) 244
GluI GCNGC 6 cut(s) 106, 259, 262, 301, 322, 331
GsaI CCCAGC 1 cut(s) 15
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 3 cut(s) 112, 618, 1280
HapII CCGG 1 cut(s) 113
HgaI GACGC 1 cut(s) 347
HhaI GCGC 1 cut(s) 245
Hin1II CATG 8 cut(s) 38, 122, 160, 229, 623, 1162, 1199, 1285
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HincII GTYRAC 1 cut(s) 1310
HindII GTYRAC 1 cut(s) 1310
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 7 cut(s) 68, 525, 881, 1080, 1091, 1098, 1231
HpaII CCGG 1 cut(s) 113
HphI GGTGA 2 cut(s) 661, 885
Hpy166II GTNNAC 3 cut(s) 94, 893, 1310
Hpy188I TCNGA 2 cut(s) 886, 1301
Hpy188III TCNNGA 7 cut(s) 72, 215, 833, 955, 1088, 1095, 1106
Hpy8I GTNNAC 3 cut(s) 94, 893, 1310
Hpy99I CGWCG 1 cut(s) 1311
HpyAV CCTTC 5 cut(s) 284, 549, 713, 1014, 1094
HpyCH4III ACNGT 6 cut(s) 79, 403, 596, 815, 952, 970
HpyCH4IV ACGT 1 cut(s) 976
HpyCH4V TGCA 7 cut(s) 192, 283, 455, 946, 973, 1189, 1288
HpyF10VI GCNNNNNNNGC 4 cut(s) 327, 330, 461, 761
HpyF3I CTNAG 5 cut(s) 216, 311, 875, 1093, 1120
HpySE526I ACGT 1 cut(s) 976
Hsp92II CATG 8 cut(s) 38, 122, 160, 229, 623, 1162, 1199, 1285
HspAI GCGC 1 cut(s) 243
Kzo9I GATC 5 cut(s) 22, 499, 658, 1161, 1265
LmnI GCTCC 3 cut(s) 269, 518, 1183
Lsp1109I GCAGC 4 cut(s) 270, 312, 333, 342
LweI GCATC 6 cut(s) 341, 403, 473, 1165, 1275, 1324
MabI ACCWGGT 1 cut(s) 248
MaeI CTAG 3 cut(s) 1037, 1106, 1263
MaeII ACGT 1 cut(s) 976
MaeIII GTNAC 6 cut(s) 47, 335, 587, 629, 649, 964
MalI GATC 5 cut(s) 24, 501, 660, 1163, 1267
MbiI CCGCTC 1 cut(s) 264
MboI GATC 5 cut(s) 22, 499, 658, 1161, 1265
MboII GAAGA 3 cut(s) 812, 1029, 1127
MhlI GDGCHC 1 cut(s) 21
MlsI TGGCCA 1 cut(s) 1280
MluCI AATT 5 cut(s) 269, 377, 699, 803, 1215
MluNI TGGCCA 1 cut(s) 1280
MlyI GAGTC 2 cut(s) 519, 1085
Mox20I TGGCCA 1 cut(s) 1280
MscI TGGCCA 1 cut(s) 1280
MseI TTAA 4 cut(s) 380, 405, 822, 1065
MslI CAYNNNNRTG 2 cut(s) 1157, 1194
Msp20I TGGCCA 1 cut(s) 1280
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 5 cut(s) 114, 250, 373, 510, 517
Mva1269I GAATGC 1 cut(s) 782
MvaI CCWGG 4 cut(s) 250, 373, 510, 517
MwoI GCNNNNNNNGC 4 cut(s) 327, 330, 461, 761
NciI CCSGG 1 cut(s) 114
NcoI CCATGG 2 cut(s) 225, 619
NdeII GATC 5 cut(s) 22, 499, 658, 1161, 1265
NlaIII CATG 8 cut(s) 38, 122, 160, 229, 623, 1162, 1199, 1285
NlaIV GGNNCC 3 cut(s) 222, 396, 514
NmuCI GTSAC 2 cut(s) 335, 649
NspI RCATGY 1 cut(s) 122
NspV TTCGAA 1 cut(s) 717
PctI GAATGC 1 cut(s) 782
PfeI GAWTC 5 cut(s) 68, 881, 1080, 1098, 1231
PflFI GACNNNGTC 1 cut(s) 1305
PflMI CCANNNNNTGG 1 cut(s) 509
PfoI TCCNGGA 1 cut(s) 515
PkrI GCNGC 6 cut(s) 107, 260, 263, 302, 323, 332
PleI GAGTC 2 cut(s) 519, 1085
PpsI GAGTC 2 cut(s) 519, 1085
PshBI ATTAAT 1 cut(s) 380
PsiI TTATAA 2 cut(s) 794, 1220
Psp124BI GAGCTC 1 cut(s) 21
Psp6I CCWGG 4 cut(s) 248, 371, 508, 515
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 4 cut(s) 248, 371, 508, 515
PspN4I GGNNCC 3 cut(s) 222, 396, 514
PspPI GGNCC 2 cut(s) 110, 616
PsyI GACNNNGTC 1 cut(s) 1305
PvuII CAGCTG 1 cut(s) 303
RsaI GTAC 4 cut(s) 817, 865, 901, 1033
RsaNI GTAC 4 cut(s) 816, 864, 900, 1032
RseI CAYNNNNRTG 2 cut(s) 1157, 1194
SacI GAGCTC 1 cut(s) 21
SalI GTCGAC 1 cut(s) 1308
SaqAI TTAA 4 cut(s) 380, 405, 822, 1065
SatI GCNGC 6 cut(s) 106, 259, 262, 301, 322, 331
Sau3AI GATC 5 cut(s) 22, 499, 658, 1161, 1265
Sau96I GGNCC 2 cut(s) 110, 616
SchI GAGTC 2 cut(s) 519, 1085
ScrFI CCNGG 5 cut(s) 114, 250, 373, 510, 517
SduI GDGCHC 1 cut(s) 21
SexAI ACCWGGT 1 cut(s) 248
SfaNI GCATC 6 cut(s) 341, 403, 473, 1165, 1275, 1324
SfcI CTRYAG 1 cut(s) 399
SfuI TTCGAA 1 cut(s) 717
SmiMI CAYNNNNRTG 2 cut(s) 1157, 1194
SmlI CTYRAG 2 cut(s) 40, 179
SmoI CTYRAG 2 cut(s) 40, 179
Sse9I AATT 5 cut(s) 269, 377, 699, 803, 1215
SsiI CCGC 4 cut(s) 105, 108, 262, 328
SspMI CTAG 3 cut(s) 1037, 1106, 1263
SstI GAGCTC 1 cut(s) 21
StyD4I CCNGG 5 cut(s) 112, 248, 371, 508, 515
StyI CCWWGG 2 cut(s) 225, 619
TaaI ACNGT 6 cut(s) 79, 403, 596, 815, 952, 970
TaiI ACGT 1 cut(s) 979
TaqI TCGA 8 cut(s) 21, 73, 489, 717, 834, 1078, 1183, 1309
TaqII GACCGA 1 cut(s) 1064
TasI AATT 5 cut(s) 269, 377, 699, 803, 1215
TatI WGTACW 1 cut(s) 899
TauI GCSGC 2 cut(s) 108, 264
TfiI GAWTC 5 cut(s) 68, 881, 1080, 1098, 1231
Tru1I TTAA 4 cut(s) 380, 405, 822, 1065
Tru9I TTAA 4 cut(s) 380, 405, 822, 1065
TseFI GTSAC 2 cut(s) 335, 649
TseI GCWGC 4 cut(s) 258, 300, 321, 330
Tsp45I GTSAC 2 cut(s) 335, 649
TspDTI ATGAA 5 cut(s) 144, 692, 905, 1212, 1271
TspGWI ACGGA 1 cut(s) 1019
Tth111I GACNNNGTC 1 cut(s) 1305
Van91I CCANNNNNTGG 1 cut(s) 509
VspI ATTAAT 1 cut(s) 380
XagI CCTNNNNNAGG 1 cut(s) 717
XbaI TCTAGA 1 cut(s) 1105
XceI RCATGY 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 1309
XspI CTAG 3 cut(s) 1037, 1106, 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.