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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 77 |
| AfaI | GTAC | 1 cut(s) | 151 |
| AgsI | TTSAA | 1 cut(s) | 56 |
| ApoI | RAATTY | 1 cut(s) | 77 |
| BseGI | GGATG | 1 cut(s) | 12 |
| BseMII | CTCAG | 1 cut(s) | 33 |
| BspCNI | CTCAG | 1 cut(s) | 32 |
| BstDEI | CTNAG | 1 cut(s) | 19 |
| BstF5I | GGATG | 1 cut(s) | 12 |
| BstNSI | RCATGY | 1 cut(s) | 48 |
| BtsCI | GGATG | 1 cut(s) | 12 |
| Csp6I | GTAC | 1 cut(s) | 150 |
| CviAII | CATG | 2 cut(s) | 45, 153 |
| CviJI | RGCY | 1 cut(s) | 23 |
| CviKI_1 | RGCY | 1 cut(s) | 23 |
| CviQI | GTAC | 1 cut(s) | 150 |
| DdeI | CTNAG | 1 cut(s) | 19 |
| FaeI | CATG | 2 cut(s) | 48, 156 |
| FaiI | YATR | 6 cut(s) | 46, 84, 104, 106, 118, 154 |
| FatI | CATG | 2 cut(s) | 44, 152 |
| FauNDI | CATATG | 1 cut(s) | 104 |
| FokI | GGATG | 1 cut(s) | 19 |
| Hin1II | CATG | 2 cut(s) | 48, 156 |
| Hpy188I | TCNGA | 1 cut(s) | 142 |
| Hpy188III | TCNNGA | 1 cut(s) | 74 |
| HpyAV | CCTTC | 1 cut(s) | 97 |
| HpyCH4V | TGCA | 2 cut(s) | 102, 108 |
| HpyF3I | CTNAG | 1 cut(s) | 19 |
| Hsp92II | CATG | 2 cut(s) | 48, 156 |
| LmnI | GCTCC | 1 cut(s) | 12 |
| LpnPI | CCDG | 1 cut(s) | 87 |
| MluCI | AATT | 1 cut(s) | 77 |
| NdeI | CATATG | 1 cut(s) | 104 |
| NlaIII | CATG | 2 cut(s) | 48, 156 |
| NspI | RCATGY | 1 cut(s) | 48 |
| RsaI | GTAC | 1 cut(s) | 151 |
| RsaNI | GTAC | 1 cut(s) | 150 |
| SetI | ASST | 1 cut(s) | 89 |
| SgeI | CNNG | 6 cut(s) | 44, 50, 57, 86, 124, 140 |
| Sse9I | AATT | 1 cut(s) | 77 |
| SspI | AATATT | 1 cut(s) | 70 |
| TasI | AATT | 1 cut(s) | 77 |
| TatI | WGTACW | 1 cut(s) | 149 |
| XapI | RAATTY | 1 cut(s) | 77 |
| XceI | RCATGY | 1 cut(s) | 48 |