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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 5 cut(s) | 31, 68, 71, 100, 136 |
| AcoI | YGGCCR | 3 cut(s) | 81, 336, 363 |
| AfaI | GTAC | 1 cut(s) | 226 |
| AhlI | ACTAGT | 1 cut(s) | 215 |
| AluBI | AGCT | 1 cut(s) | 182 |
| AluI | AGCT | 1 cut(s) | 182 |
| Alw21I | GWGCWC | 1 cut(s) | 38 |
| AoxI | GGCC | 4 cut(s) | 81, 282, 336, 363 |
| AsuHPI | GGTGA | 2 cut(s) | 290, 340 |
| BalI | TGGCCA | 1 cut(s) | 365 |
| BanII | GRGCYC | 1 cut(s) | 111 |
| Bbv12I | GWGCWC | 1 cut(s) | 38 |
| BccI | CCATC | 1 cut(s) | 122 |
| BceAI | ACGGC | 1 cut(s) | 68 |
| BcuI | ACTAGT | 1 cut(s) | 215 |
| BfaI | CTAG | 1 cut(s) | 216 |
| BisI | GCNGC | 3 cut(s) | 69, 72, 101 |
| BlsI | GCNGC | 3 cut(s) | 70, 73, 102 |
| BmcAI | AGTACT | 1 cut(s) | 226 |
| BpmI | CTGGAG | 1 cut(s) | 278 |
| BsaBI | GATNNNNATC | 1 cut(s) | 175 |
| BsaWI | WCCGGW | 1 cut(s) | 52 |
| Bse1I | ACTGG | 2 cut(s) | 32, 261 |
| Bse8I | GATNNNNATC | 1 cut(s) | 175 |
| BseJI | GATNNNNATC | 1 cut(s) | 175 |
| BseNI | ACTGG | 2 cut(s) | 32, 261 |
| BseYI | CCCAGC | 1 cut(s) | 103 |
| BshFI | GGCC | 4 cut(s) | 83, 284, 338, 365 |
| BsiHKAI | GWGCWC | 1 cut(s) | 38 |
| BsiSI | CCGG | 2 cut(s) | 53, 339 |
| BslFI | GGGAC | 1 cut(s) | 220 |
| BsmFI | GGGAC | 1 cut(s) | 220 |
| BsnI | GGCC | 4 cut(s) | 83, 284, 338, 365 |
| Bsp1286I | GDGCHC | 2 cut(s) | 38, 111 |
| Bsp143I | GATC | 2 cut(s) | 163, 229 |
| BspACI | CCGC | 5 cut(s) | 31, 68, 71, 100, 136 |
| BspANI | GGCC | 4 cut(s) | 83, 284, 338, 365 |
| BsrI | ACTGG | 2 cut(s) | 32, 261 |
| BssMI | GATC | 2 cut(s) | 163, 229 |
| Bst4CI | ACNGT | 2 cut(s) | 20, 211 |
| BstC8I | GCNNGC | 1 cut(s) | 286 |
| BstKTI | GATC | 2 cut(s) | 166, 232 |
| BstMBI | GATC | 2 cut(s) | 163, 229 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 80, 106 |
| BsuRI | GGCC | 4 cut(s) | 83, 284, 338, 365 |
| Cac8I | GCNNGC | 1 cut(s) | 286 |
| Csp6I | GTAC | 1 cut(s) | 225 |
| CviJI | RGCY | 8 cut(s) | 83, 103, 109, 152, 182, 284, 338, 365 |
| CviKI_1 | RGCY | 8 cut(s) | 83, 103, 109, 152, 182, 284, 338, 365 |
| CviQI | GTAC | 1 cut(s) | 225 |
| DpnI | GATC | 2 cut(s) | 165, 231 |
| DpnII | GATC | 2 cut(s) | 163, 229 |
| EaeI | YGGCCR | 3 cut(s) | 81, 336, 363 |
| Eco24I | GRGCYC | 1 cut(s) | 111 |
| EcoT38I | GRGCYC | 1 cut(s) | 111 |
| FaqI | GGGAC | 1 cut(s) | 220 |
| Fnu4HI | GCNGC | 3 cut(s) | 69, 72, 101 |
| FriOI | GRGCYC | 1 cut(s) | 111 |
| Fsp4HI | GCNGC | 3 cut(s) | 69, 72, 101 |
| FspBI | CTAG | 1 cut(s) | 216 |
| GluI | GCNGC | 3 cut(s) | 69, 72, 101 |
| GsaI | CCCAGC | 1 cut(s) | 107 |
| GsuI | CTGGAG | 1 cut(s) | 278 |
| HaeIII | GGCC | 4 cut(s) | 83, 284, 338, 365 |
| HapII | CCGG | 2 cut(s) | 53, 339 |
| HpaII | CCGG | 2 cut(s) | 53, 339 |
| HphI | GGTGA | 2 cut(s) | 290, 340 |
| Hpy188I | TCNGA | 2 cut(s) | 243, 313 |
| Hpy188III | TCNNGA | 1 cut(s) | 118 |
| Hpy99I | CGWCG | 2 cut(s) | 11, 317 |
| HpyAV | CCTTC | 2 cut(s) | 293, 367 |
| HpyCH4III | ACNGT | 2 cut(s) | 20, 211 |
| HpyCH4V | TGCA | 2 cut(s) | 4, 294 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 80, 106 |
| Kzo9I | GATC | 2 cut(s) | 163, 229 |
| LpnPI | CCDG | 7 cut(s) | 13, 66, 89, 103, 242, 298, 352 |
| MaeI | CTAG | 1 cut(s) | 216 |
| MaeIII | GTNAC | 2 cut(s) | 20, 211 |
| MalI | GATC | 2 cut(s) | 165, 231 |
| MboI | GATC | 2 cut(s) | 163, 229 |
| MhlI | GDGCHC | 2 cut(s) | 38, 111 |
| MlsI | TGGCCA | 1 cut(s) | 365 |
| MluNI | TGGCCA | 1 cut(s) | 365 |
| MmeI | TCCRAC | 1 cut(s) | 336 |
| MnlI | CCTC | 2 cut(s) | 132, 274 |
| Mox20I | TGGCCA | 1 cut(s) | 365 |
| MscI | TGGCCA | 1 cut(s) | 365 |
| MseI | TTAA | 1 cut(s) | 267 |
| MslI | CAYNNNNRTG | 2 cut(s) | 9, 371 |
| Msp20I | TGGCCA | 1 cut(s) | 365 |
| MspI | CCGG | 2 cut(s) | 53, 339 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 80, 106 |
| NdeII | GATC | 2 cut(s) | 163, 229 |
| NmeAIII | GCCGAG | 1 cut(s) | 17 |
| PkrI | GCNGC | 3 cut(s) | 70, 73, 102 |
| PspFI | CCCAGC | 1 cut(s) | 103 |
| RsaI | GTAC | 1 cut(s) | 226 |
| RsaNI | GTAC | 1 cut(s) | 225 |
| RseI | CAYNNNNRTG | 2 cut(s) | 9, 371 |
| SaqAI | TTAA | 1 cut(s) | 267 |
| SatI | GCNGC | 3 cut(s) | 69, 72, 101 |
| Sau3AI | GATC | 2 cut(s) | 163, 229 |
| ScaI | AGTACT | 1 cut(s) | 226 |
| SduI | GDGCHC | 2 cut(s) | 38, 111 |
| SetI | ASST | 4 cut(s) | 184, 324, 355, 359 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 9, 371 |
| SpeI | ACTAGT | 1 cut(s) | 215 |
| SsiI | CCGC | 5 cut(s) | 31, 68, 71, 100, 136 |
| SspMI | CTAG | 1 cut(s) | 216 |
| TaaI | ACNGT | 2 cut(s) | 20, 211 |
| TatI | WGTACW | 1 cut(s) | 224 |
| TauI | GCSGC | 3 cut(s) | 71, 74, 103 |
| Tru1I | TTAA | 1 cut(s) | 267 |
| Tru9I | TTAA | 1 cut(s) | 267 |
| TspDTI | ATGAA | 1 cut(s) | 227 |
| XspI | CTAG | 1 cut(s) | 216 |
| ZrmI | AGTACT | 1 cut(s) | 226 |