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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 320 |
| Acc36I | ACCTGC | 1 cut(s) | 312 |
| AccII | CGCG | 1 cut(s) | 506 |
| AciI | CCGC | 1 cut(s) | 68 |
| AcoI | YGGCCR | 1 cut(s) | 300 |
| AcsI | RAATTY | 2 cut(s) | 346, 524 |
| AdeI | CACNNNGTG | 1 cut(s) | 106 |
| AfaI | GTAC | 3 cut(s) | 172, 277, 587 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 146, 677 |
| AgeI | ACCGGT | 1 cut(s) | 5 |
| AgsI | TTSAA | 4 cut(s) | 530, 569, 611, 633 |
| AhlI | ACTAGT | 1 cut(s) | 113 |
| AluBI | AGCT | 1 cut(s) | 543 |
| AluI | AGCT | 1 cut(s) | 543 |
| Alw26I | GTCTC | 1 cut(s) | 291 |
| AoxI | GGCC | 1 cut(s) | 300 |
| ApeKI | GCWGC | 1 cut(s) | 316 |
| ApoI | RAATTY | 2 cut(s) | 346, 524 |
| AsiGI | ACCGGT | 1 cut(s) | 5 |
| AspLEI | GCGC | 2 cut(s) | 190, 321 |
| AspS9I | GGNCC | 1 cut(s) | 668 |
| AsuHPI | GGTGA | 3 cut(s) | 118, 257, 464 |
| AvaII | GGWCC | 1 cut(s) | 668 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 361, 394 |
| BbsI | GAAGAC | 2 cut(s) | 205, 651 |
| BbvI | GCAGC | 1 cut(s) | 303 |
| BccI | CCATC | 2 cut(s) | 236, 449 |
| BceAI | ACGGC | 1 cut(s) | 315 |
| BcoDI | GTCTC | 1 cut(s) | 291 |
| BcuI | ACTAGT | 1 cut(s) | 113 |
| BfaI | CTAG | 3 cut(s) | 114, 329, 597 |
| BfuAI | ACCTGC | 1 cut(s) | 312 |
| BisI | GCNGC | 1 cut(s) | 317 |
| BlsI | GCNGC | 1 cut(s) | 318 |
| BmcAI | AGTACT | 2 cut(s) | 172, 587 |
| Bme18I | GGWCC | 1 cut(s) | 668 |
| BmgT120I | GGNCC | 1 cut(s) | 668 |
| BmrI | ACTGGG | 1 cut(s) | 686 |
| BmuI | ACTGGG | 1 cut(s) | 686 |
| BpiI | GAAGAC | 2 cut(s) | 205, 651 |
| BsaJI | CCNNGG | 1 cut(s) | 183 |
| BsaWI | WCCGGW | 1 cut(s) | 5 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 146, 677 |
| Bse118I | RCCGGY | 1 cut(s) | 5 |
| Bse1I | ACTGG | 4 cut(s) | 394, 572, 670, 681 |
| Bse3DI | GCAATG | 2 cut(s) | 249, 565 |
| BseDI | CCNNGG | 1 cut(s) | 183 |
| BseGI | GGATG | 1 cut(s) | 247 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 146, 677 |
| BseMI | GCAATG | 2 cut(s) | 249, 565 |
| BseMII | CTCAG | 1 cut(s) | 42 |
| BseNI | ACTGG | 4 cut(s) | 394, 572, 670, 681 |
| BseXI | GCAGC | 1 cut(s) | 303 |
| BsgI | GTGCAG | 1 cut(s) | 404 |
| Bsh1236I | CGCG | 1 cut(s) | 506 |
| Bsh1285I | CGRYCG | 1 cut(s) | 510 |
| BshFI | GGCC | 1 cut(s) | 302 |
| BshTI | ACCGGT | 1 cut(s) | 5 |
| BsiEI | CGRYCG | 1 cut(s) | 510 |
| BsiSI | CCGG | 1 cut(s) | 6 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 146, 677 |
| BsmAI | GTCTC | 1 cut(s) | 291 |
| BsnI | GGCC | 1 cut(s) | 302 |
| Bsp143I | GATC | 1 cut(s) | 507 |
| Bsp68I | TCGCGA | 1 cut(s) | 506 |
| BspACI | CCGC | 1 cut(s) | 68 |
| BspANI | GGCC | 1 cut(s) | 302 |
| BspCNI | CTCAG | 1 cut(s) | 43 |
| BspFNI | CGCG | 1 cut(s) | 506 |
| BspMI | ACCTGC | 1 cut(s) | 312 |
| BsrDI | GCAATG | 2 cut(s) | 249, 565 |
| BsrFI | RCCGGY | 1 cut(s) | 5 |
| BsrI | ACTGG | 4 cut(s) | 394, 572, 670, 681 |
| BssAI | RCCGGY | 1 cut(s) | 5 |
| BssECI | CCNNGG | 1 cut(s) | 183 |
| BssMI | GATC | 1 cut(s) | 507 |
| BssT1I | CCWWGG | 1 cut(s) | 183 |
| Bst4CI | ACNGT | 4 cut(s) | 36, 481, 585, 590 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 466 |
| BstDEI | CTNAG | 1 cut(s) | 51 |
| BstF5I | GGATG | 1 cut(s) | 247 |
| BstFNI | CGCG | 1 cut(s) | 506 |
| BstHHI | GCGC | 2 cut(s) | 190, 321 |
| BstKTI | GATC | 1 cut(s) | 510 |
| BstMAI | GTCTC | 1 cut(s) | 291 |
| BstMBI | GATC | 1 cut(s) | 507 |
| BstMCI | CGRYCG | 1 cut(s) | 510 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 466 |
| BstUI | CGCG | 1 cut(s) | 506 |
| BstV1I | GCAGC | 1 cut(s) | 303 |
| BstV2I | GAAGAC | 2 cut(s) | 205, 651 |
| BsuRI | GGCC | 1 cut(s) | 302 |
| BtsCI | GGATG | 1 cut(s) | 247 |
| BtsI | GCAGTG | 1 cut(s) | 162 |
| BtsIMutI | CAGTG | 2 cut(s) | 162, 579 |
| BtuMI | TCGCGA | 1 cut(s) | 506 |
| BveI | ACCTGC | 1 cut(s) | 312 |
| CfoI | GCGC | 2 cut(s) | 190, 321 |
| Cfr10I | RCCGGY | 1 cut(s) | 5 |
| Cfr13I | GGNCC | 1 cut(s) | 668 |
| CseI | GACGC | 2 cut(s) | 248, 490 |
| Csp6I | GTAC | 3 cut(s) | 171, 276, 586 |
| CspAI | ACCGGT | 1 cut(s) | 5 |
| CviAII | CATG | 2 cut(s) | 43, 96 |
| CviJI | RGCY | 3 cut(s) | 74, 302, 543 |
| CviKI_1 | RGCY | 3 cut(s) | 74, 302, 543 |
| CviQI | GTAC | 3 cut(s) | 171, 276, 586 |
| DdeI | CTNAG | 1 cut(s) | 51 |
| DpnI | GATC | 1 cut(s) | 509 |
| DpnII | GATC | 1 cut(s) | 507 |
| DraIII | CACNNNGTG | 1 cut(s) | 106 |
| EaeI | YGGCCR | 1 cut(s) | 300 |
| Eco130I | CCWWGG | 1 cut(s) | 183 |
| Eco47I | GGWCC | 1 cut(s) | 668 |
| EcoT14I | CCWWGG | 1 cut(s) | 183 |
| EcoT22I | ATGCAT | 1 cut(s) | 471 |
| ErhI | CCWWGG | 1 cut(s) | 183 |
| FaeI | CATG | 2 cut(s) | 46, 99 |
| FatI | CATG | 2 cut(s) | 42, 95 |
| Fnu4HI | GCNGC | 1 cut(s) | 317 |
| FokI | GGATG | 1 cut(s) | 254 |
| Fsp4HI | GCNGC | 1 cut(s) | 317 |
| FspBI | CTAG | 3 cut(s) | 114, 329, 597 |
| FspI | TGCGCA | 1 cut(s) | 320 |
| GlaI | GCGC | 2 cut(s) | 189, 320 |
| GluI | GCNGC | 1 cut(s) | 317 |
| HaeIII | GGCC | 1 cut(s) | 302 |
| HapII | CCGG | 1 cut(s) | 6 |
| HgaI | GACGC | 2 cut(s) | 248, 490 |
| HhaI | GCGC | 2 cut(s) | 190, 321 |
| Hin1II | CATG | 2 cut(s) | 46, 99 |
| Hin6I | GCGC | 2 cut(s) | 188, 319 |
| HinP1I | GCGC | 2 cut(s) | 188, 319 |
| HinfI | GANTC | 3 cut(s) | 124, 230, 404 |
| HpaII | CCGG | 1 cut(s) | 6 |
| HphI | GGTGA | 3 cut(s) | 118, 257, 464 |
| Hpy166II | GTNNAC | 1 cut(s) | 627 |
| Hpy188I | TCNGA | 2 cut(s) | 235, 396 |
| Hpy188III | TCNNGA | 5 cut(s) | 119, 197, 263, 475, 505 |
| Hpy8I | GTNNAC | 1 cut(s) | 627 |
| Hpy99I | CGWCG | 3 cut(s) | 264, 436, 506 |
| HpyAV | CCTTC | 2 cut(s) | 506, 667 |
| HpyCH4III | ACNGT | 4 cut(s) | 36, 481, 585, 590 |
| HpyCH4V | TGCA | 5 cut(s) | 46, 254, 385, 469, 558 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 466 |
| HpyF3I | CTNAG | 1 cut(s) | 51 |
| Hsp92II | CATG | 2 cut(s) | 46, 99 |
| HspAI | GCGC | 2 cut(s) | 188, 319 |
| Kzo9I | GATC | 1 cut(s) | 507 |
| Lsp1109I | GCAGC | 1 cut(s) | 303 |
| MaeI | CTAG | 3 cut(s) | 114, 329, 597 |
| MaeIII | GTNAC | 2 cut(s) | 245, 324 |
| MalI | GATC | 1 cut(s) | 509 |
| MboI | GATC | 1 cut(s) | 507 |
| MboII | GAAGA | 4 cut(s) | 67, 205, 590, 656 |
| MluCI | AATT | 3 cut(s) | 346, 444, 524 |
| MnlI | CCTC | 2 cut(s) | 144, 356 |
| Mph1103I | ATGCAT | 1 cut(s) | 471 |
| MseI | TTAA | 1 cut(s) | 293 |
| MspI | CCGG | 1 cut(s) | 6 |
| MvnI | CGCG | 1 cut(s) | 506 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 466 |
| NdeII | GATC | 1 cut(s) | 507 |
| NlaIII | CATG | 2 cut(s) | 46, 99 |
| NmuCI | GTSAC | 1 cut(s) | 245 |
| NruI | TCGCGA | 1 cut(s) | 506 |
| NsbI | TGCGCA | 1 cut(s) | 320 |
| NsiI | ATGCAT | 1 cut(s) | 471 |
| PfeI | GAWTC | 3 cut(s) | 124, 230, 404 |
| PinAI | ACCGGT | 1 cut(s) | 5 |
| PkrI | GCNGC | 1 cut(s) | 318 |
| Ple19I | CGATCG | 1 cut(s) | 510 |
| PspPI | GGNCC | 1 cut(s) | 668 |
| PvuI | CGATCG | 1 cut(s) | 510 |
| RruI | TCGCGA | 1 cut(s) | 506 |
| RsaI | GTAC | 3 cut(s) | 172, 277, 587 |
| RsaNI | GTAC | 3 cut(s) | 171, 276, 586 |
| SaqAI | TTAA | 1 cut(s) | 293 |
| SatI | GCNGC | 1 cut(s) | 317 |
| Sau3AI | GATC | 1 cut(s) | 507 |
| Sau96I | GGNCC | 1 cut(s) | 668 |
| ScaI | AGTACT | 2 cut(s) | 172, 587 |
| SetI | ASST | 5 cut(s) | 108, 326, 440, 545, 639 |
| SinI | GGWCC | 1 cut(s) | 668 |
| SpeI | ACTAGT | 1 cut(s) | 113 |
| Sse9I | AATT | 3 cut(s) | 346, 444, 524 |
| SsiI | CCGC | 1 cut(s) | 68 |
| SspMI | CTAG | 3 cut(s) | 114, 329, 597 |
| StyI | CCWWGG | 1 cut(s) | 183 |
| TaaI | ACNGT | 4 cut(s) | 36, 481, 585, 590 |
| TaqI | TCGA | 3 cut(s) | 262, 411, 510 |
| TasI | AATT | 3 cut(s) | 346, 444, 524 |
| TatI | WGTACW | 2 cut(s) | 170, 585 |
| TfiI | GAWTC | 3 cut(s) | 124, 230, 404 |
| Tru1I | TTAA | 1 cut(s) | 293 |
| Tru9I | TTAA | 1 cut(s) | 293 |
| TscAI | CASTG | 2 cut(s) | 162, 579 |
| TseFI | GTSAC | 1 cut(s) | 245 |
| TseI | GCWGC | 1 cut(s) | 316 |
| Tsp45I | GTSAC | 1 cut(s) | 245 |
| TspDTI | ATGAA | 6 cut(s) | 68, 74, 78, 344, 537, 591 |
| TspRI | CASTG | 2 cut(s) | 162, 579 |
| VpaK11BI | GGWCC | 1 cut(s) | 668 |
| XapI | RAATTY | 2 cut(s) | 346, 524 |
| XspI | CTAG | 3 cut(s) | 114, 329, 597 |
| ZrmI | AGTACT | 2 cut(s) | 172, 587 |
| Zsp2I | ATGCAT | 1 cut(s) | 471 |