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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 86 |
| Acc36I | ACCTGC | 1 cut(s) | 86 |
| AccB1I | GGYRCC | 1 cut(s) | 74 |
| AccBSI | CCGCTC | 1 cut(s) | 358 |
| AciI | CCGC | 1 cut(s) | 358 |
| AclWI | GGATC | 1 cut(s) | 311 |
| AcsI | RAATTY | 2 cut(s) | 300, 758 |
| AfaI | GTAC | 2 cut(s) | 156, 339 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 71, 499, 586 |
| AgsI | TTSAA | 6 cut(s) | 98, 161, 250, 609, 801, 848 |
| AluBI | AGCT | 5 cut(s) | 42, 260, 391, 483, 603 |
| AluI | AGCT | 5 cut(s) | 42, 260, 391, 483, 603 |
| Alw21I | GWGCWC | 1 cut(s) | 398 |
| Alw26I | GTCTC | 1 cut(s) | 425 |
| AlwI | GGATC | 1 cut(s) | 311 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 391 |
| AoxI | GGCC | 2 cut(s) | 501, 833 |
| ApeKI | GCWGC | 1 cut(s) | 483 |
| ApoI | RAATTY | 2 cut(s) | 300, 758 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 21 |
| AspS9I | GGNCC | 2 cut(s) | 501, 833 |
| AsuHPI | GGTGA | 3 cut(s) | 70, 131, 745 |
| BaeGI | GKGCMC | 1 cut(s) | 77 |
| BanI | GGYRCC | 1 cut(s) | 74 |
| Bbv12I | GWGCWC | 1 cut(s) | 398 |
| BbvI | GCAGC | 1 cut(s) | 470 |
| BccI | CCATC | 1 cut(s) | 104 |
| BceAI | ACGGC | 3 cut(s) | 331, 431, 488 |
| BciVI | GTATCC | 1 cut(s) | 820 |
| BclI | TGATCA | 1 cut(s) | 661 |
| BcoDI | GTCTC | 1 cut(s) | 425 |
| BfaI | CTAG | 2 cut(s) | 228, 689 |
| BfuAI | ACCTGC | 1 cut(s) | 86 |
| BfuI | GTATCC | 1 cut(s) | 820 |
| BisI | GCNGC | 1 cut(s) | 484 |
| BlsI | GCNGC | 1 cut(s) | 485 |
| BmcAI | AGTACT | 1 cut(s) | 156 |
| BmgT120I | GGNCC | 2 cut(s) | 501, 833 |
| BmiI | GGNNCC | 1 cut(s) | 76 |
| BmrI | ACTGGG | 1 cut(s) | 840 |
| BmuI | ACTGGG | 1 cut(s) | 840 |
| BpmI | CTGGAG | 1 cut(s) | 673 |
| BsaJI | CCNNGG | 1 cut(s) | 504 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 71, 499, 586 |
| Bse1I | ACTGG | 3 cut(s) | 152, 656, 835 |
| BseDI | CCNNGG | 1 cut(s) | 504 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 71, 499, 586 |
| BseMII | CTCAG | 1 cut(s) | 389 |
| BseNI | ACTGG | 3 cut(s) | 152, 656, 835 |
| BseSI | GKGCMC | 1 cut(s) | 77 |
| BseXI | GCAGC | 1 cut(s) | 470 |
| BseYI | CCCAGC | 1 cut(s) | 256 |
| BshFI | GGCC | 2 cut(s) | 503, 835 |
| BshNI | GGYRCC | 1 cut(s) | 74 |
| BsiHKAI | GWGCWC | 1 cut(s) | 398 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 71, 499, 586 |
| BsmAI | GTCTC | 1 cut(s) | 425 |
| BsmI | GAATGC | 1 cut(s) | 560 |
| BsnI | GGCC | 2 cut(s) | 503, 835 |
| Bsp1286I | GDGCHC | 2 cut(s) | 77, 398 |
| Bsp143I | GATC | 2 cut(s) | 316, 661 |
| BspACI | CCGC | 1 cut(s) | 358 |
| BspANI | GGCC | 2 cut(s) | 503, 835 |
| BspCNI | CTCAG | 1 cut(s) | 388 |
| BspHI | TCATGA | 1 cut(s) | 139 |
| BspLI | GGNNCC | 1 cut(s) | 76 |
| BspMI | ACCTGC | 1 cut(s) | 86 |
| BspPI | GGATC | 1 cut(s) | 311 |
| BspT107I | GGYRCC | 1 cut(s) | 74 |
| BsrBI | CCGCTC | 1 cut(s) | 358 |
| BsrI | ACTGG | 3 cut(s) | 152, 656, 835 |
| BssECI | CCNNGG | 1 cut(s) | 504 |
| BssMI | GATC | 2 cut(s) | 316, 661 |
| Bst4CI | ACNGT | 3 cut(s) | 166, 174, 468 |
| BstC8I | GCNNGC | 2 cut(s) | 30, 40 |
| BstDEI | CTNAG | 1 cut(s) | 375 |
| BstDSI | CCRYGG | 1 cut(s) | 504 |
| BstEII | GGTNACC | 1 cut(s) | 683 |
| BstKTI | GATC | 2 cut(s) | 319, 664 |
| BstMAI | GTCTC | 1 cut(s) | 425 |
| BstMBI | GATC | 2 cut(s) | 316, 661 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 557 |
| BstPI | GGTNACC | 1 cut(s) | 683 |
| BstSLI | GKGCMC | 1 cut(s) | 77 |
| BstV1I | GCAGC | 1 cut(s) | 470 |
| BstX2I | RGATCY | 1 cut(s) | 316 |
| BstYI | RGATCY | 1 cut(s) | 316 |
| BsuI | GTATCC | 1 cut(s) | 820 |
| BsuRI | GGCC | 2 cut(s) | 503, 835 |
| BtgI | CCRYGG | 1 cut(s) | 504 |
| BveI | ACCTGC | 1 cut(s) | 86 |
| Cac8I | GCNNGC | 2 cut(s) | 30, 40 |
| CaiI | CAGNNNCTG | 1 cut(s) | 391 |
| CciI | TCATGA | 1 cut(s) | 139 |
| Cfr13I | GGNCC | 2 cut(s) | 501, 833 |
| Csp6I | GTAC | 2 cut(s) | 155, 338 |
| CviAII | CATG | 4 cut(s) | 140, 281, 330, 547 |
| CviQI | GTAC | 2 cut(s) | 155, 338 |
| DdeI | CTNAG | 1 cut(s) | 375 |
| DpnI | GATC | 2 cut(s) | 318, 663 |
| DpnII | GATC | 2 cut(s) | 316, 661 |
| Eco32I | GATATC | 1 cut(s) | 820 |
| Eco91I | GGTNACC | 1 cut(s) | 683 |
| EcoO65I | GGTNACC | 1 cut(s) | 683 |
| EcoRV | GATATC | 1 cut(s) | 820 |
| FaeI | CATG | 4 cut(s) | 143, 284, 333, 550 |
| FatI | CATG | 4 cut(s) | 139, 280, 329, 546 |
| FbaI | TGATCA | 1 cut(s) | 661 |
| Fnu4HI | GCNGC | 1 cut(s) | 484 |
| Fsp4HI | GCNGC | 1 cut(s) | 484 |
| FspBI | CTAG | 2 cut(s) | 228, 689 |
| GluI | GCNGC | 1 cut(s) | 484 |
| GsaI | CCCAGC | 1 cut(s) | 260 |
| GsuI | CTGGAG | 1 cut(s) | 673 |
| HaeIII | GGCC | 2 cut(s) | 503, 835 |
| Hin1II | CATG | 4 cut(s) | 143, 284, 333, 550 |
| HincII | GTYRAC | 1 cut(s) | 740 |
| HindII | GTYRAC | 1 cut(s) | 740 |
| HinfI | GANTC | 1 cut(s) | 61 |
| HphI | GGTGA | 3 cut(s) | 70, 131, 745 |
| Hpy166II | GTNNAC | 3 cut(s) | 177, 514, 740 |
| Hpy188I | TCNGA | 5 cut(s) | 8, 316, 412, 494, 765 |
| Hpy188III | TCNNGA | 3 cut(s) | 140, 223, 706 |
| Hpy8I | GTNNAC | 3 cut(s) | 177, 514, 740 |
| Hpy99I | CGWCG | 2 cut(s) | 722, 725 |
| HpyAV | CCTTC | 2 cut(s) | 119, 584 |
| HpyCH4III | ACNGT | 3 cut(s) | 166, 174, 468 |
| HpyCH4V | TGCA | 1 cut(s) | 89 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 557 |
| HpyF3I | CTNAG | 1 cut(s) | 375 |
| Hsp92II | CATG | 4 cut(s) | 143, 284, 333, 550 |
| Ksp22I | TGATCA | 1 cut(s) | 661 |
| Kzo9I | GATC | 2 cut(s) | 316, 661 |
| LmnI | GCTCC | 1 cut(s) | 265 |
| Lsp1109I | GCAGC | 1 cut(s) | 470 |
| MaeI | CTAG | 2 cut(s) | 228, 689 |
| MaeIII | GTNAC | 3 cut(s) | 58, 362, 683 |
| MalI | GATC | 2 cut(s) | 318, 663 |
| MbiI | CCGCTC | 1 cut(s) | 358 |
| MboI | GATC | 2 cut(s) | 316, 661 |
| MboII | GAAGA | 2 cut(s) | 29, 134 |
| MflI | RGATCY | 1 cut(s) | 316 |
| MhlI | GDGCHC | 2 cut(s) | 77, 398 |
| MluCI | AATT | 6 cut(s) | 104, 201, 300, 758, 787, 848 |
| MlyI | GAGTC | 1 cut(s) | 55 |
| MmeI | TCCRAC | 1 cut(s) | 609 |
| MnlI | CCTC | 7 cut(s) | 64, 406, 580, 753, 767, 805, 817 |
| MroXI | GAANNNNTTC | 1 cut(s) | 21 |
| MseI | TTAA | 2 cut(s) | 623, 857 |
| Mva1269I | GAATGC | 1 cut(s) | 560 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 557 |
| NdeII | GATC | 2 cut(s) | 316, 661 |
| NlaIII | CATG | 4 cut(s) | 143, 284, 333, 550 |
| NlaIV | GGNNCC | 1 cut(s) | 76 |
| NmuCI | GTSAC | 2 cut(s) | 58, 362 |
| PagI | TCATGA | 1 cut(s) | 139 |
| PaqCI | CACCTGC | 1 cut(s) | 86 |
| PctI | GAATGC | 1 cut(s) | 560 |
| PdmI | GAANNNNTTC | 1 cut(s) | 21 |
| PkrI | GCNGC | 1 cut(s) | 485 |
| PleI | GAGTC | 1 cut(s) | 55 |
| PpsI | GAGTC | 1 cut(s) | 55 |
| PspEI | GGTNACC | 1 cut(s) | 683 |
| PspFI | CCCAGC | 1 cut(s) | 256 |
| PspN4I | GGNNCC | 1 cut(s) | 76 |
| PspPI | GGNCC | 2 cut(s) | 501, 833 |
| PstNI | CAGNNNCTG | 1 cut(s) | 391 |
| PsuI | RGATCY | 1 cut(s) | 316 |
| RsaI | GTAC | 2 cut(s) | 156, 339 |
| RsaNI | GTAC | 2 cut(s) | 155, 338 |
| SaqAI | TTAA | 2 cut(s) | 623, 857 |
| SatI | GCNGC | 1 cut(s) | 484 |
| Sau3AI | GATC | 2 cut(s) | 316, 661 |
| Sau96I | GGNCC | 2 cut(s) | 501, 833 |
| ScaI | AGTACT | 1 cut(s) | 156 |
| SchI | GAGTC | 1 cut(s) | 55 |
| SduI | GDGCHC | 2 cut(s) | 77, 398 |
| Sse9I | AATT | 6 cut(s) | 104, 201, 300, 758, 787, 848 |
| SsiI | CCGC | 1 cut(s) | 358 |
| SspMI | CTAG | 2 cut(s) | 228, 689 |
| TaaI | ACNGT | 3 cut(s) | 166, 174, 468 |
| TaqI | TCGA | 4 cut(s) | 216, 354, 717, 822 |
| TasI | AATT | 6 cut(s) | 104, 201, 300, 758, 787, 848 |
| TatI | WGTACW | 1 cut(s) | 154 |
| Tru1I | TTAA | 2 cut(s) | 623, 857 |
| Tru9I | TTAA | 2 cut(s) | 623, 857 |
| TseFI | GTSAC | 2 cut(s) | 58, 362 |
| TseI | GCWGC | 1 cut(s) | 483 |
| Tsp45I | GTSAC | 2 cut(s) | 58, 362 |
| TspDTI | ATGAA | 5 cut(s) | 30, 156, 471, 665, 781 |
| XapI | RAATTY | 2 cut(s) | 300, 758 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 264, 544 |
| XmnI | GAANNNNTTC | 1 cut(s) | 21 |
| XspI | CTAG | 2 cut(s) | 228, 689 |
| ZrmI | AGTACT | 1 cut(s) | 156 |