FvH4_7g33702

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000154
Physical Location & Seq
Forward (+)
23915385 .. 23918802
3418 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g33702.t1

Sequence Viewer

Length: 2409 bp
ATGGAGAGTGATTTCCAGACGCTCAGCCTTAGTTCAAAGAATTCTGGACAAGCTAGTTCTTCTCAAGGCAAAAGCAATGCGCCTAAGAAGAATTGGAAAGGCAAAAACAAGAAGTGGGAAAAGAAGAGCAGCAATGAAGAAAGGGGTAGCTCAAGTAACGGGGTTATGGTGAAATGCAAAACATGTGACGAGTTTCACCTTGGTGCCTGTTGGTTTAAAGGAAAAGCAAAATGTCAGACTTGTGACAGGTTTGGGCATGTAGCAAAAGATTGCAACCGAAACAAACACCAAGCTCATTTCACAGAAGAACAAGATGATGATGATGATGGCAACATGTTCTTTGCTTGTCAAGCAGCAACTATAGCAAAAGATGAAAGCATCTGGTTTGTTGACAGTGGCTGCAGCAACCATATGACAGCAAATGAGTCCATTCTTATGGACATGGATACGTCAGTGAGCAGAAGGGTAAAGATGAGCAATGGTCAATTGGCACAAGCAAGAGGAAGAGGAACACTTGTTGTTGAAACAAAGAAAGGAAGAAGATTTATAAGAGAAGTGTTGTTGGTTCCAGAATTGGATCAAAACCTTCTAAGTGTTGGGCAACTTCAACATAATGGTTACCCAGTACTTTTTGATGAAGATTTGTGCACAATCTATGACAAGAAAAAGAATATGCAGATTGTGGCAACCATCAAGATGGAGAAAAACAGATGCTTTCCACTCTCTCTCAAATATCCAAGTGACATGGCTTTGAAGGTTGATGTGCTAGATGACTCTTGGTTTTGGCATAGGAGGTTTGGGCATCTAAATTTTCAGAGTTTGAAGCATCTACAGCAGAGGAATATGGTACATGGTTTACCAGCAATTCAAGAAGGAAATGAGGTGTGTGAAGGTTGTGCCATTGGGAAGCATCACCGAGAATCATTTCCTAAAGGGAAAGCTTGGAGATCAACTACACCATTGGAACTTGTTCATACTGATGTGTGTGGTGTCCTACAAAAGCAGTGGAAGAAAAAAACACCATTTGAAGCATGGAGTGGTAGAAGACCATCCGTTAATCACTTCAAGGTGTTCGGTTCTGTTTGTTATGCTTATGTTCCGAAAGAAACAAGGCATAAACTTGATGATAAAAGTGAGAAGTGTATTTTTGTGGGGTATAGCACACAAAGCAAAGGCTATAGACTCTACAATTTGAAGAAAAAGAAGGTGATAATCCGAAGAGATGTTATCTTCAATGAAAAAGCCTCTTGGGATTGGAAGCAAAAGGTTGTCCAAGGTCCTAATCTTGAAATGGAAGAAGAGGTAGAGAATCAAGAAGATGAAGGGGAAGTTGTTGGAGGAAGTCCTCAACAAGTGACTCCAATTGGCAGTCCACAATCACAATCATCCGGTTCTTCATCACCAAGCACAACTCCGATTAGAATGAGGGAGTTGAGCGAAGTCTATGCAAGCTGCAATTTCTGTGTCATGGAACCAGAAAACTTTGAAGAGGCAGTGCAAGATGAAGCTTGGAAGAAAGCTATGGTGGAAGAGATAAATGTGATTGAGAAGAATAGCACATGGGAGCTTGTGGACAGACCAATTGACAAAGAAGTCATTGGAGTGAAGTGGATTTACAAGTCAAAGTTGAATCCAGATGGTTCCATTCAAAGGAACAAAGCAAGGCTGGTTGCAAAAGGGTATGCACAACAACCCGGAATTGATTACAATGAAACATTTGCACCGGTGGCAAGGCTTGATACAATAAGGAGTGTGATCTCCTTGGCTGCTCAAAAAGGTTGGTCTCTACACCAACTTGATGTGAAATCTGCATTCCTAAATGGAGTCCTAAAGGAAGAGGTCTATGTTGATCAACCGGAGGGCTTTGTTATCAAGGGAGAAGAGATAAAGGTGTACAAGTTGAAGAAAGCTCTTTATGGCTTGAAACAAGCACCAAGAGCATGTGATTGGGGAGGTAGTGTTGATGATTCAAAGAGTACTTCTGGCTATGCTTTCACTCTTGGAACAGGTGTGTTCTCATGGCAGTCCAAGAAGCAAAAGACAGTTGCTTTGTCTACTGCTGAAGCTGAGTATGTTTCAGCATCTTTGGCAACCTCTCAAGCAATATGGCTGAGAAGTATCATGGAAGATATTGGTGAAAAGCAAGGAGAACCTACTCTAATACTGTGTAACAACAAATCTGCAATTGCGATGGCTAAGAATCCGGAGTTTCACAACCGAACCAAGCACATAGCTCGCAAGCATCACTTCATCAGAGATGCTGTTGAAGACAAAGAAATTGATGTCATATATTGCAGGACAGAAGATCAAGTTGCTGATATTTTCACCAAGGCATTGCCAAAAGACAAGTTTGAGTATTTCAGAGAATTACTGGGAGTCAAGAAGCAAAGCATTAAGGGGGAGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4219 RVT_2 Retrotran_gag_2 gag_pre-integrs rve zf-CCHC

Protein Analysis

803

Amino Acids

90.87

Weight (kDa)

8.71

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 128 - 207 1.3e-23 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 239 - 304 6.2e-16 GAG-pre-integrase domain
SH3_retrovirus PF25597 360 - 422 3.3e-28 Retroviral polymerase SH3-like domain
RVT_2 PF07727 518 - 655 9.9e-48 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000154)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02351 FvH4_1g18383 FvH4_1g18442 FvH4_1g19622 FvH4_2g08148 FvH4_2g16312 FvH4_3g06912 FvH4_3g10761 FvH4_3g12332 FvH4_3g12332 FvH4_3g14182 FvH4_3g21021 FvH4_3g35231 FvH4_3g45132 FvH4_4g08663 FvH4_4g30271 FvH4_4g30272 FvH4_4g30901 FvH4_4g30902 FvH4_5g12471 FvH4_5g22951 FvH4_6g07331 FvH4_6g07332 FvH4_6g41872 FvH4_6g43841 FvH4_6g43842 FvH4_6g48811 FvH4_7g03911 FvH4_7g13272 FvH4_7g21181 FvH4_7g29024 FvH4_7g33702
malus_domestica MD00G1037700.v1.1 MD01G1037300.v1.1 MD01G1096700.v1.1 MD01G1101100.v1.1 MD01G1129400.v1.1 MD02G1295200.v1.1 MD03G1131300.v1.1 MD04G1072100.v1.1 MD05G1104800.v1.1 MD07G1028600.v1.1 MD07G1274600.v1.1 MD08G1027200.v1.1 MD09G1044400.v1.1 MD09G1044700.v1.1 MD09G1129500.v1.1 MD09G1238700.v1.1 MD10G1011700.v1.1 MD10G1071400.v1.1 MD10G1108600.v1.1 MD10G1162100.v1.1 MD10G1162300.v1.1 MD11G1016700.v1.1 MD11G1074500.v1.1 MD15G1058600.v1.1 MD15G1383000.v1.1 MD16G1192200.v1.1 MD17G1077400.v1.1 MD17G1077500.v1.1
pyrus_communis pycom01g03520 pycom02g00350 pycom03g09910 pycom03g13960 pycom03g13970 pycom03g19180 pycom04g08410 pycom04g10140 pycom06g03750 pycom07g09240 pycom07g09260 pycom08g20130 pycom09g11020 pycom10g12690 pycom14g04140 pycom14g05710 pycom16g06380 pycom17g05500 pycom808g00140
rosa_chinensis RchiOBHm_Chr1g0316211 RchiOBHm_Chr1g0319901 RchiOBHm_Chr1g0321811 RchiOBHm_Chr1g0329831 RchiOBHm_Chr1g0330721 RchiOBHm_Chr1g0330731 RchiOBHm_Chr1g0368461 RchiOBHm_Chr1g0381111 RchiOBHm_Chr2g0154461 RchiOBHm_Chr2g0164521 RchiOBHm_Chr2g0175301 RchiOBHm_Chr3g0451301 RchiOBHm_Chr3g0453691 RchiOBHm_Chr3g0470191 RchiOBHm_Chr3g0486561 RchiOBHm_Chr4g0409141 RchiOBHm_Chr4g0409151 RchiOBHm_Chr4g0421231 RchiOBHm_Chr4g0440701 RchiOBHm_Chr5g0018211 RchiOBHm_Chr5g0023611 RchiOBHm_Chr5g0045971 RchiOBHm_Chr5g0056061 RchiOBHm_Chr5g0063381 RchiOBHm_Chr5g0064011 RchiOBHm_Chr5g0064021 RchiOBHm_Chr5g0064041 RchiOBHm_Chr6g0259681 RchiOBHm_Chr6g0259691 RchiOBHm_Chr6g0296531 RchiOBHm_Chr7g0186421 RchiOBHm_Chr7g0192321 RchiOBHm_Chr7g0198271 RchiOBHm_Chr7g0198951 RchiOBHm_Chr7g0237911
rosa_laevigata RLG00000026946
rosa_roxburghii Rroxscaffold_1G00014460 Rroxscaffold_1G00014610 Rroxscaffold_1G00028950 Rroxscaffold_1G00052630 Rroxscaffold_2G00104760 Rroxscaffold_2G00138900 Rroxscaffold_2G00145210 Rroxscaffold_3G00220110 Rroxscaffold_4G00297860 Rroxscaffold_4G00321570 Rroxscaffold_5G00332990 Rroxscaffold_5G00360870
rosa_samantha Rh1AG319600 Rh1CG299100 Rh4DG385900 Rh5BG032200
rosa_wichuraiana Rw0G002380 Rw0G003050 Rw0G003940 Rw1G002730 Rw1G003260 Rw1G005490 Rw1G016140 Rw1G016150 Rw1G017930 Rw1G017940 Rw1G022540 Rw1G022630 Rw1G031080 Rw1G039680 Rw2G003340 Rw2G003720 Rw2G006640 Rw2G020790 Rw2G025650 Rw2G028430 Rw2G029550 Rw2G034920 Rw2G040820 Rw2G048420 Rw2G048570 Rw3G010040 Rw3G022790 Rw3G023350 Rw3G025140 Rw3G028810 Rw4G000680 Rw4G003350 Rw4G004640 Rw4G026650 Rw4G034500 Rw5G008490 Rw5G020650 Rw5G023000 Rw5G033610 Rw5G037280 Rw5G046620 Rw5G050250 Rw6G001410 Rw6G005690 Rw6G005820 Rw6G010920 Rw6G022790 Rw6G023940 Rw6G026290 Rw6G028610 Rw6G031260 Rw6G036550 Rw6G036560 Rw7G015300 Rw7G017250 Rw7G018150 Rw7G031960 Rw7G036490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 548
AasI GACNNNNNNGTC 1 cut(s) 1592
AccB1I GGYRCC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 2054
AccIII TCCGGA 1 cut(s) 2203
AclWI GGATC 1 cut(s) 585
AcsI RAATTY 2 cut(s) 40, 808
AcuI CTGAAG 1 cut(s) 2082
AfaI GTAC 4 cut(s) 627, 849, 1895, 1978
AfiI CCNNNNNNNGG 1 cut(s) 1650
AflIII ACRYGT 2 cut(s) 182, 333
AgeI ACCGGT 1 cut(s) 1723
AleI CACNNNNGTG 1 cut(s) 201
Alw21I GWGCWC 1 cut(s) 650
Alw26I GTCTC 1 cut(s) 1788
Alw44I GTGCAC 1 cut(s) 646
AlwI GGATC 1 cut(s) 585
AlwNI CAGNNNCTG 1 cut(s) 399
Aor13HI TCCGGA 1 cut(s) 2203
ApaLI GTGCAC 1 cut(s) 646
ApeKI GCWGC 6 cut(s) 129, 353, 399, 402, 1452, 1766
ApoI RAATTY 2 cut(s) 40, 808
ArsI GACNNNNNNTTYG 2 cut(s) 2032, 2064
AsiGI ACCGGT 1 cut(s) 1723
Asp700I GAANNNNTTC 2 cut(s) 924, 969
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 1 cut(s) 1277
AsuC2I CCSGG 1 cut(s) 1695
AsuHPI GGTGA 7 cut(s) 181, 188, 905, 1219, 1392, 2147, 2317
AvaII GGWCC 1 cut(s) 1277
BaeGI GKGCMC 1 cut(s) 650
BaeI ACNNNNGTAYC 2 cut(s) 438, 471
BanI GGYRCC 1 cut(s) 203
BarI GAAGNNNNNNTAC 2 cut(s) 1598, 1630
BbsI GAAGAC 2 cut(s) 1051, 2274
Bbv12I GWGCWC 1 cut(s) 650
BbvI GCAGC 6 cut(s) 141, 365, 386, 414, 1439, 1753
BccI CCATC 6 cut(s) 320, 691, 698, 1057, 1631, 2185
BcgI CGANNNNNNTGC 4 cut(s) 1427, 1461, 2216, 2250
BciVI GTATCC 1 cut(s) 439
BclI TGATCA 1 cut(s) 1849
BcnI CCSGG 1 cut(s) 1695
BcoDI GTCTC 1 cut(s) 1788
BfaI CTAG 2 cut(s) 54, 767
BfmI CTRYAG 4 cut(s) 360, 400, 830, 1177
BfuI GTATCC 1 cut(s) 439
BisI GCNGC 6 cut(s) 130, 354, 400, 403, 1453, 1767
BlpI GCTNAGC 1 cut(s) 23
BlsI GCNGC 6 cut(s) 131, 355, 401, 404, 1454, 1768
BmcAI AGTACT 2 cut(s) 627, 1978
Bme1390I CCNGG 1 cut(s) 1695
Bme18I GGWCC 1 cut(s) 1277
BmgT120I GGNCC 1 cut(s) 1277
BmiI GGNNCC 4 cut(s) 205, 567, 1473, 1642
BmrFI CCNGG 1 cut(s) 1695
BmrI ACTGGG 2 cut(s) 617, 2381
BmsI GCATC 8 cut(s) 387, 701, 811, 835, 919, 2090, 2248, 2251
BmuI ACTGGG 2 cut(s) 617, 2381
BpiI GAAGAC 2 cut(s) 1051, 2274
Bpu1102I GCTNAGC 1 cut(s) 23
BpuEI CTTGAG 3 cut(s) 48, 136, 2082
BpuMI CCSGG 1 cut(s) 1695
BsaI GGTCTC 1 cut(s) 1788
BsaJI CCNNGG 4 cut(s) 199, 1273, 1761, 2328
BsaWI WCCGGW 4 cut(s) 1388, 1723, 1855, 2203
BsaXI ACNNNNNCTCC 2 cut(s) 1944, 1974
Bsc4I CCNNNNNNNGG 1 cut(s) 1650
Bse118I RCCGGY 1 cut(s) 1723
Bse1I ACTGG 2 cut(s) 623, 2376
Bse3DI GCAATG 4 cut(s) 82, 139, 484, 2333
BseAI TCCGGA 1 cut(s) 2203
BseDI CCNNGG 4 cut(s) 199, 1273, 1761, 2328
BseGI GGATG 2 cut(s) 1049, 1385
BseLI CCNNNNNNNGG 1 cut(s) 1650
BseMI GCAATG 4 cut(s) 82, 139, 484, 2333
BseMII CTCAG 3 cut(s) 37, 2058, 2102
BseNI ACTGG 2 cut(s) 623, 2376
BseSI GKGCMC 1 cut(s) 650
BseXI GCAGC 6 cut(s) 141, 365, 386, 414, 1439, 1753
BshNI GGYRCC 1 cut(s) 203
BshTI ACCGGT 1 cut(s) 1723
BsiHKAI GWGCWC 1 cut(s) 650
BsiSI CCGG 5 cut(s) 1389, 1695, 1724, 1856, 2204
BslI CCNNNNNNNGG 1 cut(s) 1650
BsmAI GTCTC 1 cut(s) 1788
BsmI GAATGC 1 cut(s) 1811
Bso31I GGTCTC 1 cut(s) 1788
Bsp1286I GDGCHC 1 cut(s) 650
Bsp13I TCCGGA 1 cut(s) 2203
Bsp1407I TGTACA 1 cut(s) 1893
Bsp143I GATC 5 cut(s) 577, 947, 1755, 1849, 2305
Bsp1720I GCTNAGC 1 cut(s) 23
BspCNI CTCAG 3 cut(s) 36, 2059, 2103
BspEI TCCGGA 1 cut(s) 2203
BspLI GGNNCC 4 cut(s) 205, 567, 1473, 1642
BspMAI CTGCAG 1 cut(s) 404
BspPI GGATC 1 cut(s) 585
BspQI GCTCTTC 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 1788
BsrDI GCAATG 4 cut(s) 82, 139, 484, 2333
BsrFI RCCGGY 1 cut(s) 1723
BsrGI TGTACA 1 cut(s) 1893
BsrI ACTGG 2 cut(s) 623, 2376
BssAI RCCGGY 1 cut(s) 1723
BssECI CCNNGG 4 cut(s) 199, 1273, 1761, 2328
BssMI GATC 5 cut(s) 577, 947, 1755, 1849, 2305
BssT1I CCWWGG 4 cut(s) 199, 1273, 1761, 2328
Bst4CI ACNGT 3 cut(s) 395, 2044, 2166
Bst6I CTCTTC 8 cut(s) 119, 499, 1213, 1293, 1482, 1524, 1830, 1875
BstAUI TGTACA 1 cut(s) 1893
BstC8I GCNNGC 3 cut(s) 1450, 2236, 2240
BstDEI CTNAG 7 cut(s) 23, 29, 84, 590, 2067, 2111, 2196
BstEII GGTNACC 1 cut(s) 617
BstF5I GGATG 2 cut(s) 1049, 1385
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 5 cut(s) 580, 950, 1758, 1852, 2308
BstMAI GTCTC 1 cut(s) 1788
BstMBI GATC 5 cut(s) 577, 947, 1755, 1849, 2305
BstMWI GCNNNNNNNGC 7 cut(s) 350, 362, 832, 1167, 1727, 1937, 2087
BstNSI RCATGY 4 cut(s) 186, 260, 337, 1944
BstPI GGTNACC 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 1693
BstSFI CTRYAG 4 cut(s) 360, 400, 830, 1177
BstSLI GKGCMC 1 cut(s) 650
BstV1I GCAGC 6 cut(s) 141, 365, 386, 414, 1439, 1753
BstV2I GAAGAC 2 cut(s) 1051, 2274
BstXI CCANNNNNNTGG 2 cut(s) 436, 697
BsuI GTATCC 1 cut(s) 439
BtgZI GCGATG 1 cut(s) 2204
BtsCI GGATG 2 cut(s) 1049, 1385
BtsI GCAGTG 2 cut(s) 1010, 1500
BtsIMutI CAGTG 4 cut(s) 400, 459, 1010, 1500
Cac8I GCNNGC 3 cut(s) 1450, 2236, 2240
CaiI CAGNNNCTG 1 cut(s) 399
CfoI GCGC 1 cut(s) 82
Cfr10I RCCGGY 1 cut(s) 1723
Cfr13I GGNCC 1 cut(s) 1277
CseI GACGC 1 cut(s) 28
Csp6I GTAC 4 cut(s) 626, 848, 1894, 1977
CspAI ACCGGT 1 cut(s) 1723
CspCI CAANNNNNGTGG 2 cut(s) 986, 1021
CviQI GTAC 4 cut(s) 626, 848, 1894, 1977
DdeI CTNAG 7 cut(s) 23, 29, 84, 590, 2067, 2111, 2196
DpnI GATC 5 cut(s) 579, 949, 1757, 1851, 2307
DpnII GATC 5 cut(s) 577, 947, 1755, 1849, 2305
DraI TTTAAA 1 cut(s) 217
DrdI GACNNNNNNGTC 1 cut(s) 1592
DseDI GACNNNNNNGTC 1 cut(s) 1592
Eam1104I CTCTTC 8 cut(s) 119, 499, 1213, 1293, 1482, 1524, 1830, 1875
EarI CTCTTC 8 cut(s) 119, 499, 1213, 1293, 1482, 1524, 1830, 1875
Eco130I CCWWGG 4 cut(s) 199, 1273, 1761, 2328
Eco31I GGTCTC 1 cut(s) 1788
Eco47I GGWCC 1 cut(s) 1277
Eco57I CTGAAG 1 cut(s) 2082
Eco91I GGTNACC 1 cut(s) 617
EcoO109I RGGNCCY 1 cut(s) 1277
EcoO65I GGTNACC 1 cut(s) 617
EcoRI GAATTC 1 cut(s) 40
EcoT14I CCWWGG 4 cut(s) 199, 1273, 1761, 2328
ErhI CCWWGG 4 cut(s) 199, 1273, 1761, 2328
FalI AAGNNNNNCTT 2 cut(s) 2231, 2263
FauNDI CATATG 1 cut(s) 411
FbaI TGATCA 1 cut(s) 1849
FblI GTMKAC 1 cut(s) 2054
Fnu4HI GCNGC 6 cut(s) 130, 354, 400, 403, 1453, 1767
FokI GGATG 2 cut(s) 1036, 1372
Fsp4HI GCNGC 6 cut(s) 130, 354, 400, 403, 1453, 1767
FspBI CTAG 2 cut(s) 54, 767
GlaI GCGC 1 cut(s) 81
GluI GCNGC 6 cut(s) 130, 354, 400, 403, 1453, 1767
HapII CCGG 5 cut(s) 1389, 1695, 1724, 1856, 2204
HgaI GACGC 1 cut(s) 28
HhaI GCGC 1 cut(s) 82
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HindIII AAGCTT 2 cut(s) 939, 1506
HpaII CCGG 5 cut(s) 1389, 1695, 1724, 1856, 2204
HphI GGTGA 7 cut(s) 181, 188, 905, 1219, 1392, 2147, 2317
Hpy166II GTNNAC 7 cut(s) 391, 648, 857, 1373, 1573, 1894, 2055
Hpy188I TCNGA 7 cut(s) 237, 816, 1101, 1217, 1416, 2255, 2363
Hpy8I GTNNAC 7 cut(s) 391, 648, 857, 1373, 1573, 1894, 2055
HpyAV CCTTC 7 cut(s) 456, 596, 748, 866, 884, 1198, 1316
HpyCH4III ACNGT 3 cut(s) 395, 2044, 2166
HpyCH4IV ACGT 1 cut(s) 449
HpyF10VI GCNNNNNNNGC 7 cut(s) 350, 362, 832, 1167, 1727, 1937, 2087
HpyF3I CTNAG 7 cut(s) 23, 29, 84, 590, 2067, 2111, 2196
HpySE526I ACGT 1 cut(s) 449
HspAI GCGC 1 cut(s) 80
Kpn2I TCCGGA 1 cut(s) 2203
Ksp22I TGATCA 1 cut(s) 1849
Kzo9I GATC 5 cut(s) 577, 947, 1755, 1849, 2305
LguI GCTCTTC 1 cut(s) 119
LmnI GCTCC 1 cut(s) 1564
Lsp1109I GCAGC 6 cut(s) 141, 365, 386, 414, 1439, 1753
LweI GCATC 8 cut(s) 387, 701, 811, 835, 919, 2090, 2248, 2251
MaeI CTAG 2 cut(s) 54, 767
MaeII ACGT 1 cut(s) 449
MaeIII GTNAC 7 cut(s) 155, 185, 242, 617, 740, 1354, 2168
MalI GATC 5 cut(s) 579, 949, 1757, 1851, 2307
MboI GATC 5 cut(s) 577, 947, 1755, 1849, 2305
MfeI CAATTG 4 cut(s) 485, 1362, 1581, 2184
MhlI GDGCHC 1 cut(s) 650
MlyI GAGTC 6 cut(s) 434, 767, 1176, 1351, 1833, 2385
MmeI TCCRAC 1 cut(s) 1315
MroI TCCGGA 1 cut(s) 2203
MroXI GAANNNNTTC 2 cut(s) 924, 969
MseI TTAA 4 cut(s) 216, 1056, 2394, 2407
MslI CAYNNNNRTG 5 cut(s) 201, 434, 695, 978, 1601
MspI CCGG 5 cut(s) 1389, 1695, 1724, 1856, 2204
MspR9I CCNGG 1 cut(s) 1695
MunI CAATTG 4 cut(s) 485, 1362, 1581, 2184
Mva1269I GAATGC 1 cut(s) 1811
MwoI GCNNNNNNNGC 7 cut(s) 350, 362, 832, 1167, 1727, 1937, 2087
NciI CCSGG 1 cut(s) 1695
NdeI CATATG 1 cut(s) 411
NdeII GATC 5 cut(s) 577, 947, 1755, 1849, 2305
NlaIV GGNNCC 4 cut(s) 205, 567, 1473, 1642
NmuCI GTSAC 4 cut(s) 185, 242, 740, 1354
NspI RCATGY 4 cut(s) 186, 260, 337, 1944
OliI CACNNNNGTG 1 cut(s) 201
PciI ACATGT 2 cut(s) 182, 333
PciSI GCTCTTC 1 cut(s) 119
PctI GAATGC 1 cut(s) 1811
PdmI GAANNNNTTC 2 cut(s) 924, 969
PfeI GAWTC 5 cut(s) 920, 1309, 1630, 1967, 2200
PinAI ACCGGT 1 cut(s) 1723
PkrI GCNGC 6 cut(s) 131, 355, 401, 404, 1454, 1768
PleI GAGTC 6 cut(s) 433, 767, 1176, 1351, 1832, 2384
PpsI GAGTC 6 cut(s) 433, 767, 1176, 1351, 1832, 2384
PpuMI RGGWCCY 1 cut(s) 1277
PscI ACATGT 2 cut(s) 182, 333
PsiI TTATAA 1 cut(s) 548
Psp5II RGGWCCY 1 cut(s) 1277
PspEI GGTNACC 1 cut(s) 617
PspN4I GGNNCC 4 cut(s) 205, 567, 1473, 1642
PspPI GGNCC 1 cut(s) 1277
PspPPI RGGWCCY 1 cut(s) 1277
PstI CTGCAG 1 cut(s) 404
PstNI CAGNNNCTG 1 cut(s) 399
RsaI GTAC 4 cut(s) 627, 849, 1895, 1978
RsaNI GTAC 4 cut(s) 626, 848, 1894, 1977
RseI CAYNNNNRTG 5 cut(s) 201, 434, 695, 978, 1601
SapI GCTCTTC 1 cut(s) 119
SaqAI TTAA 4 cut(s) 216, 1056, 2394, 2407
SatI GCNGC 6 cut(s) 130, 354, 400, 403, 1453, 1767
Sau3AI GATC 5 cut(s) 577, 947, 1755, 1849, 2305
Sau96I GGNCC 1 cut(s) 1277
ScaI AGTACT 2 cut(s) 627, 1978
SchI GAGTC 6 cut(s) 434, 767, 1176, 1351, 1833, 2385
ScrFI CCNGG 1 cut(s) 1695
SduI GDGCHC 1 cut(s) 650
SfaNI GCATC 8 cut(s) 387, 701, 811, 835, 919, 2090, 2248, 2251
SfcI CTRYAG 4 cut(s) 360, 400, 830, 1177
SgrAI CRCCGGYG 1 cut(s) 1723
SinI GGWCC 1 cut(s) 1277
SmiMI CAYNNNNRTG 5 cut(s) 201, 434, 695, 978, 1601
SmlI CTYRAG 3 cut(s) 63, 151, 2097
SmoI CTYRAG 3 cut(s) 63, 151, 2097
SspMI CTAG 2 cut(s) 54, 767
StyD4I CCNGG 1 cut(s) 1693
StyI CCWWGG 4 cut(s) 199, 1273, 1761, 2328
TaaI ACNGT 3 cut(s) 395, 2044, 2166
TaiI ACGT 1 cut(s) 452
TatI WGTACW 3 cut(s) 625, 1893, 1976
TfiI GAWTC 5 cut(s) 920, 1309, 1630, 1967, 2200
Tru1I TTAA 4 cut(s) 216, 1056, 2394, 2407
Tru9I TTAA 4 cut(s) 216, 1056, 2394, 2407
TscAI CASTG 4 cut(s) 400, 459, 1010, 1500
TseFI GTSAC 4 cut(s) 185, 242, 740, 1354
TseI GCWGC 6 cut(s) 129, 353, 399, 402, 1452, 1766
Tsp45I GTSAC 4 cut(s) 185, 242, 740, 1354
TspGWI ACGGA 1 cut(s) 1042
TspRI CASTG 4 cut(s) 400, 459, 1010, 1500
VneI GTGCAC 1 cut(s) 646
VpaK11BI GGWCC 1 cut(s) 1277
XapI RAATTY 2 cut(s) 40, 808
XceI RCATGY 4 cut(s) 186, 260, 337, 1944
XcmI CCANNNNNNNNNTGG 1 cut(s) 1029
XmiI GTMKAC 1 cut(s) 2054
XmnI GAANNNNTTC 2 cut(s) 924, 969
XspI CTAG 2 cut(s) 54, 767
ZrmI AGTACT 2 cut(s) 627, 1978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.