MD09G1238700.v1.1

gag-polypeptide of LTR copia-type

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000154
Physical Location & Seq
Forward (+)
30367031 .. 30372844
5814 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1238700.v1.1.491

Sequence Viewer

Length: 3552 bp
ATGGTCGTGTATATAATGACATTAATTAATGAGAAGGAGACTCATTTCAATATATTCAAGGAAAATGTGGCGTTTATAGAATCTTCTAATAAGGATGCAAACAAACCTTACAAATTGAGTGTTAATCAATTTGCAGACCTTACAAATGAAGAGTTCAAAGCCTCAAGAAATGGATTCAAGGGACACGAGTGCTCCACGAAGACTTCTTTCAAATATGAAAATGTGACTACATCATTACCAGCAATAATGGATTGGAGAAAGAAAGGAGCAGTAACCCCTGTCAAGGACCAAGGCCAATGCGGTAGATGTTGTTGGGCTTTTTTAGCAGTGGCTGCCACGAAAGGAATTACACAGCTTATAACTGGGAAATTGATGTCTTTGTCTGAGCAAGAGCTCGTTGATTGTGACACTGCTGAAGAAACTAAAGACCTTGACTCCATTGAGGTTCAAGAAGTGATTGGTACATTGAAAGGCTATGAGCAGAGGTTAAATATGCATTCTGAAAACTTAGCTGAAAAGGCTTTTACTAGCCTAAGTGTGACTGAGAGAGATAGCAGTTACAGGGGTCAGGGGAGCAACTCAAATCAAAAGAAATTATGGAAAGGAAAAGGGAAGAAATGGGACAACAACCATACTTCACAATCAAAATCAGAATCTAGCAGTGAGTCTACCAAAACCAAGTGTAAAATCTGTGACAAATTACACTATGGTGTTTGCTGGTTCAAGGGAAAACCCAAATGCACCAAGTGTGACAGATTTGGCCATCTAGCAAAAGATTGTAATCCAAGAAGGAACCAAGTTGTCAACTATGCACATAGAGCAGAAGAAGAGGAAGTAAATGTCTTTTATGCTTGTAGTGTTGCAAAAACTGAAAACAAGAGAGGAATCTGGTATATTGACAGTGGATGCAGTAATCACATGACTGCATATGAATCCCTGCTGATCAATATTGACAGAAGCTTCAATTGCAGAGTTAAAATGGGGAATGGACAACTAGTGGAAGCTACTGGAAAAGGAACTCTTGTTCTAGAGACAAAGGGTGGAAGAAGATTTATCAAGGATGTAATACTTGTACCAGGTCTTGATGAGAATCTCCTTAGTGTGGGACAGATGATTGCTCATGGTTACTTCTTACTTTTTGGAGACAATATGGTGGAGATCTTTGATGACAGAAGCCTCCAAAACTTGGTAACACAAGTTGGCATGACAGAAAACAAAAGCTTTCCACTTATGCTAGATTACAATGATTCAATTGCTCTAAAAGCAAGTGTTGCAGAAAACTCTTGGTTATGGCATAAGAGGTTCGGACATCTCAACTTTCACAGTTTAAAGAGATTGGAGAAACTACAAATGGTGACTGGTCTTCCTGAGTTACAAGAGACTAAAGAACCGTGTGAAGGTTGCATATTGGGGAAGCATCACAAGGACTCTTTTGAAACTGGAAGTGCATGGAGAGCAAGTCAACCTCTAGAACTCATACACACAGATGTGTGTGGTCCAATGAAGACACCTACACTCAGTGGGAACAAATATTTTCTGCTATTCATTGATGACTGCACCAGGATGGTTTGGGTATATCTTATGAGAAACAAGAGTGAAGTCTTTTCAATCTTCAAGAAATTCAAAATGATGGTTGAACTTCAGAGTGGTCTCAAGATCAAAAGACTAAGAAGCGACAGAGGTGGTGAATTCACTTCACTTGAGTTCCAAGAATTCTGTGAAGGAGCAGGATTACAGAAGCAATTGACAGTGGCCTACACACCACAGCAGAATGGTGTGGCTGAGAGGAAGAACAGGACCGTGGTTGAAATGACCAAGTCAATGCTCCATGATAAGAAAATGCCACTTTCTTTTTGGGGTGAAGCTGTCAATACATCTGTGTATCTCCTAAACAGGTGTCCAACTAAAGCACTTGAGAAGAAAACATCGTTTGAGGCATTTAGTGGCAGAAAACCAAGTGTGAAGCACTTGAAGGTGTTCGGATCAATCTGCTATGCACAGATACCATCTCAACTACGACACAAGCTTGAGATCAATAGTGTCAAGTGTGTTCTTGTAGGTTATGGAAGCTGTGAAAAGGGCTATAGGCTCTACAATATTGAGTCACGTAAAGTGATTCTCTCAAGGGATGTGGTGTTCAAGGAGAATGAAGCATGGAATTGGGAAACCAATAACATAGACACTATCTCAGTTCCACTAGCAATGGAAGAAGCACAAGCTGAAGTGCAAAATGTGAATGAACCTGTTACACAAGATGAGAGTCAAACTGACTTTTCAACTCCAACACAAGGATCTCAGTCATCTACTTCAAATGCAGAAGGACAAGAATTACAGGATTCCTCCCCTGAAACAACTCCAGTGAGAATGAGGAGTTTAAATGAGATCTATGCAGTGTGTAACTATTGTGTTGTTGAACCAGAGAGTTTTGAAGAAGCTGAGAAGGATCAATCCTGGCAGAAAGCAATGAGTGAAGAAATTGCCATGATTGAGAAAAACTCTACTTGGAAATTGGTAAATAGACCGAGTGATAAACCTGTGATAGGTGTGAAATGGATTTACAAGATCAAACTGAATTTGGATGGATCAATACAGAAGAACAAAGCAAGATTAGTTGCCAAGGGCTACTCACAACAACCTGGGGTAGATTTCAATGAAACCTTTGCCCCAGTGGCAAGGTTAGACACCATTAGAACCTTGATTGCTCTTGCTGCCCAAAAGGGATGGAAACTTCACCAGTTAGATGTCAAATCAGCTTTTCTAAATGGCATTTTGGAGGAAGAAGTGTATGTGGATCAACCACAAGGATTTGAAGTGCCGAATAAAGAGGAGAAGGTCTACAAGTTAAACAAAGCACTGTATGGACTCAAACAAGCCCCCAGGGCTTGGTACAGTGAGATAGACTCCTATTTCAGCAAGAGTGGATTTAACAAAAGTCCTAGTGAAGCTACATTATATACAAAAACTGGTGAGAACTCTGAGATGCTTATTGTGTCTATATATGTTGATGATGTGGTCTTTACTGGTAATTGTGAAGAAATGATTGAAGAATTCAGAAGGGAAATGATGAGACAATATGAGATGTCGGATCTTGGCTTGTTGCATCACTTCTTAGGAATTGGAGTAATACAAGAAGCTACTGGGATTTTCATACATCAACAGAAGTATGCACAATCACTGCTTGAAAGATTCAATCTGAAAGGATGCAAATCTGTGGCAACACCTTTGATCACGAATGAGAAACTTTGTAAAGTGGATGGTTCTGAATCTGCTGATGAATGCTTATACCGAATGATGGTTGGAAGTCTGCTTTATTTGACTGCTACAAGACCTGACATTATTGGTGTCTTTACGGGAACCTGTGGAACGAGCCTTGACCATGGTGTTACCGCTGTTGGTTCTGGCGTGAGTGAGGATGAGACTAAGTATTGGCTAGTGAAGAACTCATGGGGTGCAAAATGGGGTGAAGCTGGGTACATAAGAATGCAGAGAGATGTTGCTGCGGCGGAAGGACTTTATGGCATTGCTATGGCTACCTCTTACCCCACAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4219 Retrotran_gag_2 gag_pre-integrs zf-CCHC

Protein Analysis

1184

Amino Acids

133.59

Weight (kDa)

7.06

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 10 - 52 1e-11 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 79 - 141 2.1e-17 Papain family cysteine protease
Pol_BBD PF22936 297 - 376 4.2e-22 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 417 - 473 4e-15 GAG-pre-integrase domain
rve PF00665 487 - 588 2.4e-14 Integrase core domain
SH3_retrovirus PF25597 662 - 724 1e-22 Retroviral polymerase SH3-like domain
RVT_2 PF07727 832 - 1076 4.3e-86 Reverse transcriptase (RNA-dependent DNA polymerase)
Peptidase_C1 PF00112 1114 - 1181 7.3e-17 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000154)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02351 FvH4_1g18383 FvH4_1g18442 FvH4_1g19622 FvH4_2g08148 FvH4_2g16312 FvH4_3g06912 FvH4_3g10761 FvH4_3g12332 FvH4_3g12332 FvH4_3g14182 FvH4_3g21021 FvH4_3g35231 FvH4_3g45132 FvH4_4g08663 FvH4_4g30271 FvH4_4g30272 FvH4_4g30901 FvH4_4g30902 FvH4_5g12471 FvH4_5g22951 FvH4_6g07331 FvH4_6g07332 FvH4_6g41872 FvH4_6g43841 FvH4_6g43842 FvH4_6g48811 FvH4_7g03911 FvH4_7g13272 FvH4_7g21181 FvH4_7g29024 FvH4_7g33702
malus_domestica MD00G1037700.v1.1 MD01G1037300.v1.1 MD01G1096700.v1.1 MD01G1101100.v1.1 MD01G1129400.v1.1 MD02G1295200.v1.1 MD03G1131300.v1.1 MD04G1072100.v1.1 MD05G1104800.v1.1 MD07G1028600.v1.1 MD07G1274600.v1.1 MD08G1027200.v1.1 MD09G1044400.v1.1 MD09G1044700.v1.1 MD09G1129500.v1.1 MD09G1238700.v1.1 MD10G1011700.v1.1 MD10G1071400.v1.1 MD10G1108600.v1.1 MD10G1162100.v1.1 MD10G1162300.v1.1 MD11G1016700.v1.1 MD11G1074500.v1.1 MD15G1058600.v1.1 MD15G1383000.v1.1 MD16G1192200.v1.1 MD17G1077400.v1.1 MD17G1077500.v1.1
pyrus_communis pycom01g03520 pycom02g00350 pycom03g09910 pycom03g13960 pycom03g13970 pycom03g19180 pycom04g08410 pycom04g10140 pycom06g03750 pycom07g09240 pycom07g09260 pycom08g20130 pycom09g11020 pycom10g12690 pycom14g04140 pycom14g05710 pycom16g06380 pycom17g05500 pycom808g00140
rosa_chinensis RchiOBHm_Chr1g0316211 RchiOBHm_Chr1g0319901 RchiOBHm_Chr1g0321811 RchiOBHm_Chr1g0329831 RchiOBHm_Chr1g0330721 RchiOBHm_Chr1g0330731 RchiOBHm_Chr1g0368461 RchiOBHm_Chr1g0381111 RchiOBHm_Chr2g0154461 RchiOBHm_Chr2g0164521 RchiOBHm_Chr2g0175301 RchiOBHm_Chr3g0451301 RchiOBHm_Chr3g0453691 RchiOBHm_Chr3g0470191 RchiOBHm_Chr3g0486561 RchiOBHm_Chr4g0409141 RchiOBHm_Chr4g0409151 RchiOBHm_Chr4g0421231 RchiOBHm_Chr4g0440701 RchiOBHm_Chr5g0018211 RchiOBHm_Chr5g0023611 RchiOBHm_Chr5g0045971 RchiOBHm_Chr5g0056061 RchiOBHm_Chr5g0063381 RchiOBHm_Chr5g0064011 RchiOBHm_Chr5g0064021 RchiOBHm_Chr5g0064041 RchiOBHm_Chr6g0259681 RchiOBHm_Chr6g0259691 RchiOBHm_Chr6g0296531 RchiOBHm_Chr7g0186421 RchiOBHm_Chr7g0192321 RchiOBHm_Chr7g0198271 RchiOBHm_Chr7g0198951 RchiOBHm_Chr7g0237911
rosa_laevigata RLG00000026946
rosa_roxburghii Rroxscaffold_1G00014460 Rroxscaffold_1G00014610 Rroxscaffold_1G00028950 Rroxscaffold_1G00052630 Rroxscaffold_2G00104760 Rroxscaffold_2G00138900 Rroxscaffold_2G00145210 Rroxscaffold_3G00220110 Rroxscaffold_4G00297860 Rroxscaffold_4G00321570 Rroxscaffold_5G00332990 Rroxscaffold_5G00360870
rosa_samantha Rh1AG319600 Rh1CG299100 Rh4DG385900 Rh5BG032200
rosa_wichuraiana Rw0G002380 Rw0G003050 Rw0G003940 Rw1G002730 Rw1G003260 Rw1G005490 Rw1G016140 Rw1G016150 Rw1G017930 Rw1G017940 Rw1G022540 Rw1G022630 Rw1G031080 Rw1G039680 Rw2G003340 Rw2G003720 Rw2G006640 Rw2G020790 Rw2G025650 Rw2G028430 Rw2G029550 Rw2G034920 Rw2G040820 Rw2G048420 Rw2G048570 Rw3G010040 Rw3G022790 Rw3G023350 Rw3G025140 Rw3G028810 Rw4G000680 Rw4G003350 Rw4G004640 Rw4G026650 Rw4G034500 Rw5G008490 Rw5G020650 Rw5G023000 Rw5G033610 Rw5G037280 Rw5G046620 Rw5G050250 Rw6G001410 Rw6G005690 Rw6G005820 Rw6G010920 Rw6G022790 Rw6G023940 Rw6G026290 Rw6G028610 Rw6G031260 Rw6G036550 Rw6G036560 Rw7G015300 Rw7G017250 Rw7G018150 Rw7G031960 Rw7G036490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 359
AccB7I CCANNNNNTGG 2 cut(s) 1186, 2883
AccI GTMKAC 2 cut(s) 668, 2835
AciI CCGC 4 cut(s) 300, 3387, 3500, 3503
AclWI GGATC 6 cut(s) 1990, 2299, 2451, 2590, 2799, 3093
AcoI YGGCCR 1 cut(s) 760
AcsI RAATTY 5 cut(s) 1619, 1688, 1712, 2572, 3047
AcuI CTGAAG 3 cut(s) 435, 1625, 2241
AdeI CACNNNGTG 2 cut(s) 747, 1520
AfaI GTAC 4 cut(s) 463, 1074, 2888, 3473
AfiI CCNNNNNNNGG 8 cut(s) 283, 1102, 1186, 1397, 2288, 2540, 2883, 3292
AhlI ACTAGT 1 cut(s) 994
AjnI CCWGG 5 cut(s) 1075, 1559, 2450, 2635, 2876
AleI CACNNNNGTG 2 cut(s) 708, 1487
AloI GAACNNNNNNTCC 4 cut(s) 1008, 1040, 2120, 2152
Alw21I GWGCWC 2 cut(s) 194, 396
Alw26I GTCTC 7 cut(s) 32, 1025, 1137, 1373, 1655, 3061, 3410
AlwI GGATC 6 cut(s) 1990, 2299, 2451, 2590, 2799, 3093
AlwNI CAGNNNCTG 1 cut(s) 332
AoxI GGCC 3 cut(s) 292, 760, 1752
ApeKI GCWGC 3 cut(s) 332, 2708, 3497
ApoI RAATTY 5 cut(s) 1619, 1688, 1712, 2572, 3047
AseI ATTAAT 2 cut(s) 23, 27
Asp700I GAANNNNTTC 1 cut(s) 1976
AspS9I GGNCC 3 cut(s) 286, 1496, 1797
AsuHPI GGTGA 6 cut(s) 1366, 1697, 1871, 2723, 2978, 3473
AvaII GGWCC 3 cut(s) 286, 1496, 1797
BalI TGGCCA 1 cut(s) 762
BanII GRGCYC 1 cut(s) 396
BauI CACGAG 1 cut(s) 185
BbsI GAAGAC 3 cut(s) 206, 1355, 1511
Bbv12I GWGCWC 2 cut(s) 194, 396
BbvI GCAGC 3 cut(s) 319, 2695, 3484
BccI CCATC 8 cut(s) 771, 1558, 1624, 2014, 2573, 2715, 3248, 3286
BciT130I CCWGG 5 cut(s) 1077, 1561, 2452, 2637, 2878
BclI TGATCA 2 cut(s) 942, 3225
BcoDI GTCTC 7 cut(s) 32, 1025, 1137, 1373, 1655, 3061, 3410
BcuI ACTAGT 1 cut(s) 994
BfmI CTRYAG 1 cut(s) 2083
BglI GCCNNNNNGGC 2 cut(s) 2669, 2879
BglII AGATCT 2 cut(s) 1158, 2382
BisI GCNGC 4 cut(s) 333, 2709, 3498, 3501
BlsI GCNGC 4 cut(s) 334, 2710, 3499, 3502
Bme1390I CCNGG 5 cut(s) 1077, 1561, 2452, 2637, 2878
Bme18I GGWCC 3 cut(s) 286, 1496, 1797
BmgT120I GGNCC 3 cut(s) 286, 1496, 1797
BmiI GGNNCC 2 cut(s) 794, 3355
BmrFI CCNGG 5 cut(s) 1077, 1561, 2452, 2637, 2878
BmrI ACTGGG 3 cut(s) 372, 2660, 3147
BmsI GCATC 6 cut(s) 85, 896, 1426, 2970, 3109, 3191
BmuI ACTGGG 3 cut(s) 372, 2660, 3147
BpiI GAAGAC 3 cut(s) 206, 1355, 1511
BplI GAGNNNNNCTC 4 cut(s) 2480, 2512, 2885, 2917
BpmI CTGGAG 1 cut(s) 2340
BpuEI CTTGAG 6 cut(s) 148, 1637, 1721, 1934, 2048, 2107
BsaAI YACGTR 1 cut(s) 2108
BsaBI GATNNNNATC 3 cut(s) 780, 1089, 3205
BsaI GGTCTC 1 cut(s) 1655
BsaJI CCNNGG 7 cut(s) 289, 1800, 2616, 2636, 2876, 2877, 3376
BsaXI ACNNNNNCTCC 6 cut(s) 176, 206, 419, 449, 1444, 1474
Bsc4I CCNNNNNNNGG 8 cut(s) 283, 1102, 1186, 1397, 2288, 2540, 2883, 3292
Bse3DI GCAATG 3 cut(s) 2208, 2469, 3519
Bse8I GATNNNNATC 3 cut(s) 780, 1089, 3205
BseBI CCWGG 5 cut(s) 1077, 1561, 2452, 2637, 2878
BseDI CCNNGG 7 cut(s) 289, 1800, 2616, 2636, 2876, 2877, 3376
BseJI GATNNNNATC 3 cut(s) 780, 1089, 3205
BseLI CCNNNNNNNGG 8 cut(s) 283, 1102, 1186, 1397, 2288, 2540, 2883, 3292
BseMI GCAATG 3 cut(s) 2208, 2469, 3519
BseMII CTCAG 9 cut(s) 375, 534, 1359, 1531, 1773, 2202, 2309, 2427, 2967
BseRI GAGGAG 2 cut(s) 2383, 2840
BseXI GCAGC 3 cut(s) 319, 2695, 3484
BseYI CCCAGC 1 cut(s) 3467
BsgI GTGCAG 1 cut(s) 1540
BshFI GGCC 3 cut(s) 294, 762, 1754
BsiHKAI GWGCWC 2 cut(s) 194, 396
BslFI GGGAC 3 cut(s) 195, 635, 1119
BslI CCNNNNNNNGG 8 cut(s) 283, 1102, 1186, 1397, 2288, 2540, 2883, 3292
BsmAI GTCTC 7 cut(s) 32, 1025, 1137, 1373, 1655, 3061, 3410
BsmFI GGGAC 3 cut(s) 195, 635, 1119
BsmI GAATGC 3 cut(s) 496, 3281, 3486
BsnI GGCC 3 cut(s) 294, 762, 1754
Bso31I GGTCTC 1 cut(s) 1655
Bsp1286I GDGCHC 2 cut(s) 194, 396
Bsp19I CCATGG 1 cut(s) 3376
BspACI CCGC 4 cut(s) 300, 3387, 3500, 3503
BspANI GGCC 3 cut(s) 294, 762, 1754
BspCNI CTCAG 9 cut(s) 376, 535, 1360, 1530, 1774, 2201, 2308, 2428, 2968
BspLI GGNNCC 2 cut(s) 794, 3355
BspPI GGATC 6 cut(s) 1990, 2299, 2451, 2590, 2799, 3093
BspTNI GGTCTC 1 cut(s) 1655
BsrDI GCAATG 3 cut(s) 2208, 2469, 3519
BssECI CCNNGG 7 cut(s) 289, 1800, 2616, 2636, 2876, 2877, 3376
BssSI CACGAG 1 cut(s) 185
BssT1I CCWWGG 3 cut(s) 289, 2616, 3376
Bst2BI CACGAG 1 cut(s) 185
Bst2UI CCWGG 5 cut(s) 1077, 1561, 2452, 2637, 2878
Bst4CI ACNGT 7 cut(s) 902, 1325, 1392, 1750, 1801, 2856, 2891
Bst6I CTCTTC 2 cut(s) 144, 822
BstAPI GCANNNNNTGC 2 cut(s) 332, 1271
BstBAI YACGTR 1 cut(s) 2108
BstDSI CCRYGG 2 cut(s) 1800, 3376
BstENI CCTNNNNNAGG 1 cut(s) 2538
BstMAI GTCTC 7 cut(s) 32, 1025, 1137, 1373, 1655, 3061, 3410
BstNI CCWGG 5 cut(s) 1077, 1561, 2452, 2637, 2878
BstSCI CCNGG 5 cut(s) 1075, 1559, 2450, 2635, 2876
BstSFI CTRYAG 1 cut(s) 2083
BstV1I GCAGC 3 cut(s) 319, 2695, 3484
BstV2I GAAGAC 3 cut(s) 206, 1355, 1511
BstX2I RGATCY 4 cut(s) 1158, 2291, 2382, 3085
BstYI RGATCY 4 cut(s) 1158, 2291, 2382, 3085
BsuRI GGCC 3 cut(s) 294, 762, 1754
BtgI CCRYGG 2 cut(s) 1800, 3376
BtsI GCAGTG 5 cut(s) 333, 408, 667, 2397, 3173
CaiI CAGNNNCTG 1 cut(s) 332
Cfr13I GGNCC 3 cut(s) 286, 1496, 1797
CsiI ACCWGGT 1 cut(s) 1075
Csp6I GTAC 4 cut(s) 462, 1073, 2887, 3472
CspCI CAANNNNNGTGG 2 cut(s) 2112, 2147
CviAII CATG 9 cut(s) 919, 1121, 1204, 1449, 1829, 2154, 2482, 3377, 3444
CviQI GTAC 4 cut(s) 462, 1073, 2887, 3472
DraI TTTAAA 2 cut(s) 1329, 2376
DraIII CACNNNGTG 2 cut(s) 747, 1520
EaeI YGGCCR 1 cut(s) 760
Eam1104I CTCTTC 2 cut(s) 144, 822
EarI CTCTTC 2 cut(s) 144, 822
EciI GGCGGA 1 cut(s) 3518
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 3 cut(s) 289, 2616, 3376
Eco24I GRGCYC 1 cut(s) 396
Eco31I GGTCTC 1 cut(s) 1655
Eco47I GGWCC 3 cut(s) 286, 1496, 1797
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 3 cut(s) 435, 1625, 2241
EcoICRI GAGCTC 1 cut(s) 394
EcoNI CCTNNNNNAGG 1 cut(s) 2538
EcoRI GAATTC 3 cut(s) 1688, 1712, 3047
EcoRII CCWGG 5 cut(s) 1075, 1559, 2450, 2635, 2876
EcoT14I CCWWGG 3 cut(s) 289, 2616, 3376
EcoT22I ATGCAT 1 cut(s) 498
EcoT38I GRGCYC 1 cut(s) 396
ErhI CCWWGG 3 cut(s) 289, 2616, 3376
FaeI CATG 9 cut(s) 922, 1124, 1207, 1452, 1832, 2157, 2485, 3380, 3447
FalI AAGNNNNNCTT 2 cut(s) 1005, 1037
FaqI GGGAC 3 cut(s) 195, 635, 1119
FatI CATG 9 cut(s) 918, 1120, 1203, 1448, 1828, 2153, 2481, 3376, 3443
FauNDI CATATG 1 cut(s) 928
FbaI TGATCA 2 cut(s) 942, 3225
FblI GTMKAC 2 cut(s) 668, 2835
Fnu4HI GCNGC 4 cut(s) 333, 2709, 3498, 3501
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 4 cut(s) 333, 2709, 3498, 3501
GluI GCNGC 4 cut(s) 333, 2709, 3498, 3501
GsaI CCCAGC 1 cut(s) 3471
GsuI CTGGAG 1 cut(s) 2340
HaeIII GGCC 3 cut(s) 294, 762, 1754
Hin1II CATG 9 cut(s) 922, 1124, 1207, 1452, 1832, 2157, 2485, 3380, 3447
HincII GTYRAC 2 cut(s) 805, 1463
HindII GTYRAC 2 cut(s) 805, 1463
HindIII AAGCTT 3 cut(s) 958, 1219, 2024
HphI GGTGA 6 cut(s) 1366, 1697, 1871, 2723, 2978, 3473
Hpy166II GTNNAC 4 cut(s) 669, 805, 1463, 2836
Hpy188III TCNNGA 9 cut(s) 165, 449, 1028, 1082, 1367, 1469, 1615, 1654, 3229
Hpy8I GTNNAC 4 cut(s) 669, 805, 1463, 2836
HpyCH4III ACNGT 7 cut(s) 902, 1325, 1392, 1750, 1801, 2856, 2891
HpyCH4IV ACGT 1 cut(s) 2107
HpySE526I ACGT 1 cut(s) 2107
Hsp92II CATG 9 cut(s) 922, 1124, 1207, 1452, 1832, 2157, 2485, 3380, 3447
Ksp22I TGATCA 2 cut(s) 942, 3225
LmnI GCTCC 5 cut(s) 197, 266, 573, 1724, 1830
Lsp1109I GCAGC 3 cut(s) 319, 2695, 3484
LweI GCATC 6 cut(s) 85, 896, 1426, 2970, 3109, 3191
MabI ACCWGGT 1 cut(s) 1075
MaeII ACGT 1 cut(s) 2107
MfeI CAATTG 3 cut(s) 964, 1251, 1742
MflI RGATCY 4 cut(s) 1158, 2291, 2382, 3085
MhlI GDGCHC 2 cut(s) 194, 396
MlsI TGGCCA 1 cut(s) 762
MluNI TGGCCA 1 cut(s) 762
MlyI GAGTC 8 cut(s) 34, 428, 674, 1421, 2111, 2269, 2856, 2894
MmeI TCCRAC 4 cut(s) 1925, 2306, 3063, 3277
Mox20I TGGCCA 1 cut(s) 762
Mph1103I ATGCAT 1 cut(s) 498
MroXI GAANNNNTTC 1 cut(s) 1976
MscI TGGCCA 1 cut(s) 762
MseI TTAA 9 cut(s) 23, 27, 123, 488, 975, 1328, 2375, 2843, 2925
MslI CAYNNNNRTG 6 cut(s) 708, 1485, 1487, 1562, 3479, 3524
Msp20I TGGCCA 1 cut(s) 762
MspA1I CMGCKG 1 cut(s) 3389
MspR9I CCNGG 5 cut(s) 1077, 1561, 2452, 2637, 2878
MunI CAATTG 3 cut(s) 964, 1251, 1742
Mva1269I GAATGC 3 cut(s) 496, 3281, 3486
MvaI CCWGG 5 cut(s) 1077, 1561, 2452, 2637, 2878
NcoI CCATGG 1 cut(s) 3376
NdeI CATATG 1 cut(s) 928
NlaIII CATG 9 cut(s) 922, 1124, 1207, 1452, 1832, 2157, 2485, 3380, 3447
NlaIV GGNNCC 2 cut(s) 794, 3355
NmuCI GTSAC 7 cut(s) 223, 404, 538, 692, 749, 1354, 2103
NsiI ATGCAT 1 cut(s) 498
OliI CACNNNNGTG 2 cut(s) 708, 1487
PacI TTAATTAA 1 cut(s) 27
PasI CCCWGGG 1 cut(s) 2877
PctI GAATGC 3 cut(s) 496, 3281, 3486
PdmI GAANNNNTTC 1 cut(s) 1976
PflFI GACNNNGTC 1 cut(s) 1816
PflMI CCANNNNNTGG 2 cut(s) 1186, 2883
PkrI GCNGC 4 cut(s) 334, 2710, 3499, 3502
PleI GAGTC 8 cut(s) 34, 428, 673, 1421, 2110, 2268, 2856, 2894
PpsI GAGTC 8 cut(s) 34, 428, 673, 1421, 2110, 2268, 2856, 2894
Ppu21I YACGTR 1 cut(s) 2108
PshBI ATTAAT 2 cut(s) 23, 27
PsiI TTATAA 1 cut(s) 359
Psp124BI GAGCTC 1 cut(s) 396
Psp6I CCWGG 5 cut(s) 1075, 1559, 2450, 2635, 2876
PspFI CCCAGC 1 cut(s) 3467
PspGI CCWGG 5 cut(s) 1075, 1559, 2450, 2635, 2876
PspN4I GGNNCC 2 cut(s) 794, 3355
PspPI GGNCC 3 cut(s) 286, 1496, 1797
PstNI CAGNNNCTG 1 cut(s) 332
PsuI RGATCY 4 cut(s) 1158, 2291, 2382, 3085
PsyI GACNNNGTC 1 cut(s) 1816
RsaI GTAC 4 cut(s) 463, 1074, 2888, 3473
RsaNI GTAC 4 cut(s) 462, 1073, 2887, 3472
RseI CAYNNNNRTG 6 cut(s) 708, 1485, 1487, 1562, 3479, 3524
SacI GAGCTC 1 cut(s) 396
SaqAI TTAA 9 cut(s) 23, 27, 123, 488, 975, 1328, 2375, 2843, 2925
SatI GCNGC 4 cut(s) 333, 2709, 3498, 3501
Sau96I GGNCC 3 cut(s) 286, 1496, 1797
SchI GAGTC 8 cut(s) 34, 428, 674, 1421, 2111, 2269, 2856, 2894
ScrFI CCNGG 5 cut(s) 1077, 1561, 2452, 2637, 2878
SduI GDGCHC 2 cut(s) 194, 396
SexAI ACCWGGT 1 cut(s) 1075
SfaNI GCATC 6 cut(s) 85, 896, 1426, 2970, 3109, 3191
SfcI CTRYAG 1 cut(s) 2083
SinI GGWCC 3 cut(s) 286, 1496, 1797
SmiMI CAYNNNNRTG 6 cut(s) 708, 1485, 1487, 1562, 3479, 3524
SmlI CTYRAG 6 cut(s) 163, 1652, 1700, 1913, 2027, 2122
SmoI CTYRAG 6 cut(s) 163, 1652, 1700, 1913, 2027, 2122
SpeI ACTAGT 1 cut(s) 994
SsiI CCGC 4 cut(s) 300, 3387, 3500, 3503
SspI AATATT 3 cut(s) 949, 1532, 2098
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 5 cut(s) 1075, 1559, 2450, 2635, 2876
StyI CCWWGG 3 cut(s) 289, 2616, 3376
TaaI ACNGT 7 cut(s) 902, 1325, 1392, 1750, 1801, 2856, 2891
TaiI ACGT 1 cut(s) 2110
TaqII GACCGA 1 cut(s) 2536
TauI GCSGC 1 cut(s) 3503
Tru1I TTAA 9 cut(s) 23, 27, 123, 488, 975, 1328, 2375, 2843, 2925
Tru9I TTAA 9 cut(s) 23, 27, 123, 488, 975, 1328, 2375, 2843, 2925
TseFI GTSAC 7 cut(s) 223, 404, 538, 692, 749, 1354, 2103
TseI GCWGC 3 cut(s) 332, 2708, 3497
Tsp45I GTSAC 7 cut(s) 223, 404, 538, 692, 749, 1354, 2103
Tth111I GACNNNGTC 1 cut(s) 1816
Van91I CCANNNNNTGG 2 cut(s) 1186, 2883
VpaK11BI GGWCC 3 cut(s) 286, 1496, 1797
VspI ATTAAT 2 cut(s) 23, 27
XagI CCTNNNNNAGG 1 cut(s) 2538
XapI RAATTY 5 cut(s) 1619, 1688, 1712, 2572, 3047
XbaI TCTAGA 2 cut(s) 1027, 1468
XcmI CCANNNNNNNNNTGG 1 cut(s) 1851
XmiI GTMKAC 2 cut(s) 668, 2835
XmnI GAANNNNTTC 1 cut(s) 1976
Zsp2I ATGCAT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.