pycom01g03520

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000154
Physical Location & Seq
Forward (+)
2683389 .. 2685659
2271 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03520.6

Sequence Viewer

Length: 1734 bp
ATGAATTCACTGATTCTCAAACTGAGAAGATCATTGAGTGAAATCTATGCCAGATGCAATGTAAGCATGATTGAACCAAAGAATTTCACTAATGCTACCACTGATATAGCTTGGAAGAAAGCAATGGATGCTGAGATTGAGATGATAAAGAAAAACAAAACCTGGGAATTGGTTAACAGACCATCAGACAAACCCATTGTTGGAGTGAAATGGATCTACAAGACCAAATTGAATATGGATGGGACTATTCAGAAGTACAAGGCAAGGCTGGAAACTTTTGCTCCTGTTGCAAGATTGGATACCATCAGAACTCTTATAGCTTTAGCAGCTCAAAATAAGTGGAAGTTGTATCAATTAGATGTGAAATCAACTTTTTTGAAAGGAACTGTGCAAGGTGCAGAAGCTAAGGTCTATAAGCTTAAGAAAGCATTGTATGGTTTAAAGCAAGCTCCAAGAGCTTGGTATGAAGAAATTGATGCTCATCTGTATAATTGTGGATTCACCAGAAGTCCAAATGAGGCAACATTGTATATGAAGTCAAGGGGAGAAAATGAAAGCATCATTGTCTCTATCTATGAAGAATTTAAGTGTGACAATATGAGAATATATGAAATGATAAACTTGGGACTGCTATATCATTTCCTTGGAATGGGAATTCTTCAATCTGAATCTGGAATTTTCATTAACCAAAGGAAATATGCAGTGAGTTTACTGCAGAAATTTGGGTTGAAGGACTGCAAACCTGTTAGTATTCCATTGGTGGTGAGTGACAAGCTCAAGAAAGATGATGAGGGTGACAAAACAGATGAGAATGTATATAGGCAATTGGTGGGAAGTCTTTTGTATCTGATTGCAACCAGACCAGATATTATGTTTGCAGCTAGCTTACTTGCTAGATTCATGCATGGACCAACAAAGAAACATTTGGGAGCAACAAAGAGAGTGCTCAGATATATACAAGGCACTTTAGACTATGGAATTGAGTATGAGAAAGGCAAAGAATCAATTCTCATAGCCTACTGTGACAATGACTGGAGTGGGGATGAAACTGATATGAAAGACACATTTGGATATACTTTTGAAGGAAATATTTATGAAAACTACATGCTTGTATGCACTCAAAAACAAAACTTAACCCATGAAATTCAAAGCCGAATAGTTATGGTGAACCAAGACTCAACTCAAAACAAAGCTAATCACCCAAGAGAGAGGGCCTTCATTCCTTACTTCTTACCTCCATGGATTTCTTGGAGGAGGATGGATGAATGGATTCTCCAAGTTCCCAAAGAAGAGACAGATGCTAGTAATCTCTCCCTAGGGTGGCCGGCCTTCATTAGAGAAGAGAGAGCTTTTGTGTTCTTTTCTCTTCCCTTAGAAAACCCTAAGGATGAAATGGCTATAAAGTTCCTTTTATACCACCTCACAATGGAGTGCCCTTTGTGTTTTCAAGTTGTCATACAACTTGAGTTATTGGGCTTGACATTCAAATTCCATTGGGCCTTGAGGCCCAAAACTAACTCGAGGTCTATAACGAACTTATTTTATTCTTACTCATCTCCGTTGTTCATTTTAGCGAGGCAATGGGTGATTAGAACTCTTCAAATCGATTGTGAATTGAAACATACTTTCAATTCTCCCTTTAGTGATTATACACCTTTAGGGCTTCCACAAACCATGAAAGAGGTGGAGAACCTATTCATTTTAGGATTACAATGCAATATGGGTTTTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

578

Amino Acids

67.0

Weight (kDa)

8.85

Isoelectric Point (pI)

38.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000154)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02351 FvH4_1g18383 FvH4_1g18442 FvH4_1g19622 FvH4_2g08148 FvH4_2g16312 FvH4_3g06912 FvH4_3g10761 FvH4_3g12332 FvH4_3g12332 FvH4_3g14182 FvH4_3g21021 FvH4_3g35231 FvH4_3g45132 FvH4_4g08663 FvH4_4g30271 FvH4_4g30272 FvH4_4g30901 FvH4_4g30902 FvH4_5g12471 FvH4_5g22951 FvH4_6g07331 FvH4_6g07332 FvH4_6g41872 FvH4_6g43841 FvH4_6g43842 FvH4_6g48811 FvH4_7g03911 FvH4_7g13272 FvH4_7g21181 FvH4_7g29024 FvH4_7g33702
malus_domestica MD00G1037700.v1.1 MD01G1037300.v1.1 MD01G1096700.v1.1 MD01G1101100.v1.1 MD01G1129400.v1.1 MD02G1295200.v1.1 MD03G1131300.v1.1 MD04G1072100.v1.1 MD05G1104800.v1.1 MD07G1028600.v1.1 MD07G1274600.v1.1 MD08G1027200.v1.1 MD09G1044400.v1.1 MD09G1044700.v1.1 MD09G1129500.v1.1 MD09G1238700.v1.1 MD10G1011700.v1.1 MD10G1071400.v1.1 MD10G1108600.v1.1 MD10G1162100.v1.1 MD10G1162300.v1.1 MD11G1016700.v1.1 MD11G1074500.v1.1 MD15G1058600.v1.1 MD15G1383000.v1.1 MD16G1192200.v1.1 MD17G1077400.v1.1 MD17G1077500.v1.1
pyrus_communis pycom01g03520 pycom02g00350 pycom03g09910 pycom03g13960 pycom03g13970 pycom03g19180 pycom04g08410 pycom04g10140 pycom06g03750 pycom07g09240 pycom07g09260 pycom08g20130 pycom09g11020 pycom10g12690 pycom14g04140 pycom14g05710 pycom16g06380 pycom17g05500 pycom808g00140
rosa_chinensis RchiOBHm_Chr1g0316211 RchiOBHm_Chr1g0319901 RchiOBHm_Chr1g0321811 RchiOBHm_Chr1g0329831 RchiOBHm_Chr1g0330721 RchiOBHm_Chr1g0330731 RchiOBHm_Chr1g0368461 RchiOBHm_Chr1g0381111 RchiOBHm_Chr2g0154461 RchiOBHm_Chr2g0164521 RchiOBHm_Chr2g0175301 RchiOBHm_Chr3g0451301 RchiOBHm_Chr3g0453691 RchiOBHm_Chr3g0470191 RchiOBHm_Chr3g0486561 RchiOBHm_Chr4g0409141 RchiOBHm_Chr4g0409151 RchiOBHm_Chr4g0421231 RchiOBHm_Chr4g0440701 RchiOBHm_Chr5g0018211 RchiOBHm_Chr5g0023611 RchiOBHm_Chr5g0045971 RchiOBHm_Chr5g0056061 RchiOBHm_Chr5g0063381 RchiOBHm_Chr5g0064011 RchiOBHm_Chr5g0064021 RchiOBHm_Chr5g0064041 RchiOBHm_Chr6g0259681 RchiOBHm_Chr6g0259691 RchiOBHm_Chr6g0296531 RchiOBHm_Chr7g0186421 RchiOBHm_Chr7g0192321 RchiOBHm_Chr7g0198271 RchiOBHm_Chr7g0198951 RchiOBHm_Chr7g0237911
rosa_laevigata RLG00000026946
rosa_roxburghii Rroxscaffold_1G00014460 Rroxscaffold_1G00014610 Rroxscaffold_1G00028950 Rroxscaffold_1G00052630 Rroxscaffold_2G00104760 Rroxscaffold_2G00138900 Rroxscaffold_2G00145210 Rroxscaffold_3G00220110 Rroxscaffold_4G00297860 Rroxscaffold_4G00321570 Rroxscaffold_5G00332990 Rroxscaffold_5G00360870
rosa_samantha Rh1AG319600 Rh1CG299100 Rh4DG385900 Rh5BG032200
rosa_wichuraiana Rw0G002380 Rw0G003050 Rw0G003940 Rw1G002730 Rw1G003260 Rw1G005490 Rw1G016140 Rw1G016150 Rw1G017930 Rw1G017940 Rw1G022540 Rw1G022630 Rw1G031080 Rw1G039680 Rw2G003340 Rw2G003720 Rw2G006640 Rw2G020790 Rw2G025650 Rw2G028430 Rw2G029550 Rw2G034920 Rw2G040820 Rw2G048420 Rw2G048570 Rw3G010040 Rw3G022790 Rw3G023350 Rw3G025140 Rw3G028810 Rw4G000680 Rw4G003350 Rw4G004640 Rw4G026650 Rw4G034500 Rw5G008490 Rw5G020650 Rw5G023000 Rw5G033610 Rw5G037280 Rw5G046620 Rw5G050250 Rw6G001410 Rw6G005690 Rw6G005820 Rw6G010920 Rw6G022790 Rw6G023940 Rw6G026290 Rw6G028610 Rw6G031260 Rw6G036550 Rw6G036560 Rw7G015300 Rw7G017250 Rw7G018150 Rw7G031960 Rw7G036490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 221
AcoI YGGCCR 1 cut(s) 1322
AcsI RAATTY 8 cut(s) 4, 82, 581, 654, 675, 719, 1143, 1487
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 4 cut(s) 200, 649, 1320, 1426
AflII CTTAAG 1 cut(s) 419
AjnI CCWGG 1 cut(s) 161
Alw21I GWGCWC 1 cut(s) 948
Alw26I GTCTC 2 cut(s) 571, 1286
AlwI GGATC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 1519
AoxI GGCC 5 cut(s) 1212, 1322, 1326, 1497, 1505
ApeKI GCWGC 2 cut(s) 326, 878
ApoI RAATTY 8 cut(s) 4, 82, 581, 654, 675, 719, 1143, 1487
Asp700I GAANNNNTTC 3 cut(s) 1005, 1269, 1694
AspA2I CCTAGG 1 cut(s) 1315
AspS9I GGNCC 4 cut(s) 908, 1212, 1497, 1506
AsuHPI GGTGA 6 cut(s) 493, 775, 806, 1177, 1190, 1597
AsuNHI GCTAGC 1 cut(s) 881
AvaI CYCGRG 1 cut(s) 1519
AvaII GGWCC 1 cut(s) 908
AvrII CCTAGG 1 cut(s) 1315
AxyI CCTNAGG 1 cut(s) 1383
BaeGI GKGCMC 1 cut(s) 1436
Bbv12I GWGCWC 1 cut(s) 948
BbvI GCAGC 2 cut(s) 338, 890
BccI CCATC 4 cut(s) 190, 233, 311, 1252
BciT130I CCWGG 1 cut(s) 163
BciVI GTATCC 1 cut(s) 292
BcoDI GTCTC 2 cut(s) 571, 1286
BfaI CTAG 4 cut(s) 882, 894, 1302, 1316
BfmI CTRYAG 1 cut(s) 713
BfrI CTTAAG 1 cut(s) 419
BfuI GTATCC 1 cut(s) 292
BisI GCNGC 2 cut(s) 327, 879
BlnI CCTAGG 1 cut(s) 1315
BlsI GCNGC 2 cut(s) 328, 880
Bme1390I CCNGG 1 cut(s) 163
Bme18I GGWCC 1 cut(s) 908
BmeT110I CYCGRG 1 cut(s) 1519
BmgT120I GGNCC 4 cut(s) 908, 1212, 1497, 1506
BmrFI CCNGG 1 cut(s) 163
BmsI GCATC 5 cut(s) 44, 118, 466, 567, 1288
BmtI GCTAGC 1 cut(s) 885
BpmI CTGGAG 1 cut(s) 1054
Bpu10I CCTNAGC 1 cut(s) 405
BpuEI CTTGAG 3 cut(s) 761, 1484, 1522
Bsa29I ATCGAT 1 cut(s) 1605
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 4 cut(s) 162, 643, 1238, 1315
BsaXI ACNNNNNCTCC 2 cut(s) 265, 295
Bsc4I CCNNNNNNNGG 4 cut(s) 200, 649, 1320, 1426
Bse118I RCCGGY 1 cut(s) 1324
Bse1I ACTGG 1 cut(s) 1037
Bse21I CCTNAGG 1 cut(s) 1383
Bse3DI GCAATG 3 cut(s) 64, 129, 1586
Bse8I GATNNNNATC 1 cut(s) 480
BseBI CCWGG 1 cut(s) 163
BseCI ATCGAT 1 cut(s) 1605
BseDI CCNNGG 4 cut(s) 162, 643, 1238, 1315
BseGI GGATG 6 cut(s) 133, 244, 1048, 1263, 1267, 1393
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 4 cut(s) 200, 649, 1320, 1426
BseMI GCAATG 3 cut(s) 64, 129, 1586
BseMII CTCAG 3 cut(s) 14, 123, 961
BseNI ACTGG 1 cut(s) 1037
BseRI GAGGAG 1 cut(s) 1267
BseSI GKGCMC 1 cut(s) 1436
BseXI GCAGC 2 cut(s) 338, 890
BsgI GTGCAG 1 cut(s) 417
BshFI GGCC 5 cut(s) 1214, 1324, 1328, 1499, 1507
BshVI ATCGAT 1 cut(s) 1605
BsiHKAI GWGCWC 1 cut(s) 948
BsiHKCI CYCGRG 1 cut(s) 1519
BsiSI CCGG 1 cut(s) 1325
BslFI GGGAC 2 cut(s) 256, 639
BslI CCNNNNNNNGG 4 cut(s) 200, 649, 1320, 1426
BsmAI GTCTC 2 cut(s) 571, 1286
BsmFI GGGAC 2 cut(s) 256, 639
BsnI GGCC 5 cut(s) 1214, 1324, 1328, 1499, 1507
BsoBI CYCGRG 1 cut(s) 1519
Bsp1286I GDGCHC 2 cut(s) 948, 1436
Bsp143I GATC 2 cut(s) 29, 213
Bsp19I CCATGG 1 cut(s) 1238
BspANI GGCC 5 cut(s) 1214, 1324, 1328, 1499, 1507
BspCNI CTCAG 3 cut(s) 15, 124, 960
BspDI ATCGAT 1 cut(s) 1605
BspMAI CTGCAG 1 cut(s) 717
BspOI GCTAGC 1 cut(s) 885
BspPI GGATC 1 cut(s) 221
BspTI CTTAAG 1 cut(s) 419
BsrDI GCAATG 3 cut(s) 64, 129, 1586
BsrFI RCCGGY 1 cut(s) 1324
BsrI ACTGG 1 cut(s) 1037
BssAI RCCGGY 1 cut(s) 1324
BssECI CCNNGG 4 cut(s) 162, 643, 1238, 1315
BssMI GATC 2 cut(s) 29, 213
BssT1I CCWWGG 3 cut(s) 643, 1238, 1315
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 2 cut(s) 388, 1022
Bst6I CTCTTC 4 cut(s) 1284, 1335, 1371, 1602
BstAFI CTTAAG 1 cut(s) 419
BstAPI GCANNNNNTGC 1 cut(s) 128
BstC8I GCNNGC 3 cut(s) 447, 883, 1326
BstDEI CTNAG 6 cut(s) 23, 132, 405, 947, 1372, 1383
BstDSI CCRYGG 1 cut(s) 1238
BstF5I GGATG 6 cut(s) 133, 244, 1048, 1263, 1267, 1393
BstKTI GATC 2 cut(s) 32, 216
BstMAI GTCTC 2 cut(s) 571, 1286
BstMBI GATC 2 cut(s) 29, 213
BstMWI GCNNNNNNNGC 5 cut(s) 63, 128, 287, 326, 455
BstNI CCWGG 1 cut(s) 163
BstNSI RCATGY 1 cut(s) 1108
BstSCI CCNGG 1 cut(s) 161
BstSFI CTRYAG 1 cut(s) 713
BstSLI GKGCMC 1 cut(s) 1436
BstV1I GCAGC 2 cut(s) 338, 890
BstX2I RGATCY 1 cut(s) 213
BstXI CCANNNNNNTGG 1 cut(s) 459
BstYI RGATCY 1 cut(s) 213
Bsu15I ATCGAT 1 cut(s) 1605
Bsu36I CCTNAGG 1 cut(s) 1383
BsuI GTATCC 1 cut(s) 292
BsuRI GGCC 5 cut(s) 1214, 1324, 1328, 1499, 1507
BsuTUI ATCGAT 1 cut(s) 1605
BtgI CCRYGG 1 cut(s) 1238
BtsCI GGATG 6 cut(s) 133, 244, 1048, 1263, 1267, 1393
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 3 cut(s) 8, 99, 708
Cac8I GCNNGC 3 cut(s) 447, 883, 1326
Cfr10I RCCGGY 1 cut(s) 1324
Cfr13I GGNCC 4 cut(s) 908, 1212, 1497, 1506
ClaI ATCGAT 1 cut(s) 1605
Csp6I GTAC 1 cut(s) 256
CspCI CAANNNNNGTGG 2 cut(s) 320, 355
CviAII CATG 7 cut(s) 67, 901, 905, 1105, 1139, 1239, 1675
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 6 cut(s) 23, 132, 405, 947, 1372, 1383
DpnI GATC 2 cut(s) 31, 215
DpnII GATC 2 cut(s) 29, 213
DraI TTTAAA 1 cut(s) 441
EaeI YGGCCR 1 cut(s) 1322
Eam1104I CTCTTC 4 cut(s) 1284, 1335, 1371, 1602
EarI CTCTTC 4 cut(s) 1284, 1335, 1371, 1602
Eco130I CCWWGG 3 cut(s) 643, 1238, 1315
Eco47I GGWCC 1 cut(s) 908
Eco81I CCTNAGG 1 cut(s) 1383
Eco88I CYCGRG 1 cut(s) 1519
EcoO109I RGGNCCY 1 cut(s) 1212
EcoRI GAATTC 2 cut(s) 4, 654
EcoRII CCWGG 1 cut(s) 161
EcoT14I CCWWGG 3 cut(s) 643, 1238, 1315
EcoT22I ATGCAT 1 cut(s) 906
ErhI CCWWGG 3 cut(s) 643, 1238, 1315
FaeI CATG 7 cut(s) 70, 904, 908, 1108, 1142, 1242, 1678
FaqI GGGAC 2 cut(s) 256, 639
FatI CATG 7 cut(s) 66, 900, 904, 1104, 1138, 1238, 1674
Fnu4HI GCNGC 2 cut(s) 327, 879
FokI GGATG 6 cut(s) 140, 251, 1055, 1270, 1274, 1400
FseI GGCCGGCC 1 cut(s) 1328
Fsp4HI GCNGC 2 cut(s) 327, 879
FspBI CTAG 4 cut(s) 882, 894, 1302, 1316
GluI GCNGC 2 cut(s) 327, 879
GsuI CTGGAG 1 cut(s) 1054
HaeIII GGCC 5 cut(s) 1214, 1324, 1328, 1499, 1507
HapII CCGG 1 cut(s) 1325
Hin1II CATG 7 cut(s) 70, 904, 908, 1108, 1142, 1242, 1678
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 7 cut(s) 13, 498, 668, 897, 1001, 1175, 1270
HpaI GTTAAC 1 cut(s) 175
HpaII CCGG 1 cut(s) 1325
HphI GGTGA 6 cut(s) 493, 775, 806, 1177, 1190, 1597
Hpy166II GTNNAC 3 cut(s) 175, 710, 1168
Hpy188I TCNGA 6 cut(s) 187, 252, 308, 667, 849, 950
Hpy188III TCNNGA 2 cut(s) 672, 778
Hpy8I GTNNAC 3 cut(s) 175, 710, 1168
HpyAV CCTTC 4 cut(s) 724, 1076, 1225, 1339
HpyCH4III ACNGT 2 cut(s) 388, 1022
HpyF10VI GCNNNNNNNGC 5 cut(s) 63, 128, 287, 326, 455
HpyF3I CTNAG 6 cut(s) 23, 132, 405, 947, 1372, 1383
Hsp92II CATG 7 cut(s) 70, 904, 908, 1108, 1142, 1242, 1678
KroI GCCGGC 1 cut(s) 1324
KroNI GCCGGC 1 cut(s) 1326
KspAI GTTAAC 1 cut(s) 175
Kzo9I GATC 2 cut(s) 29, 213
LmnI GCTCC 3 cut(s) 286, 454, 929
Lsp1109I GCAGC 2 cut(s) 338, 890
LweI GCATC 5 cut(s) 44, 118, 466, 567, 1288
MaeI CTAG 4 cut(s) 882, 894, 1302, 1316
MaeIII GTNAC 4 cut(s) 590, 767, 794, 1022
MalI GATC 2 cut(s) 31, 215
MboI GATC 2 cut(s) 29, 213
MboII GAAGA 9 cut(s) 39, 127, 479, 590, 650, 1301, 1352, 1358, 1589
MfeI CAATTG 1 cut(s) 824
MflI RGATCY 1 cut(s) 213
MhlI GDGCHC 2 cut(s) 948, 1436
MlyI GAGTC 1 cut(s) 1169
MmeI TCCRAC 1 cut(s) 181
Mph1103I ATGCAT 1 cut(s) 906
MroNI GCCGGC 1 cut(s) 1324
MroXI GAANNNNTTC 3 cut(s) 1005, 1269, 1694
MseI TTAA 6 cut(s) 174, 420, 440, 585, 684, 1133
MspCI CTTAAG 1 cut(s) 419
MspI CCGG 1 cut(s) 1325
MspR9I CCNGG 1 cut(s) 163
MunI CAATTG 1 cut(s) 824
MvaI CCWGG 1 cut(s) 163
MwoI GCNNNNNNNGC 5 cut(s) 63, 128, 287, 326, 455
NaeI GCCGGC 1 cut(s) 1326
NcoI CCATGG 1 cut(s) 1238
NdeII GATC 2 cut(s) 29, 213
NgoMIV GCCGGC 1 cut(s) 1324
NheI GCTAGC 1 cut(s) 881
NlaIII CATG 7 cut(s) 70, 904, 908, 1108, 1142, 1242, 1678
NmuCI GTSAC 4 cut(s) 590, 767, 794, 1022
NsiI ATGCAT 1 cut(s) 906
NspI RCATGY 1 cut(s) 1108
PaeR7I CTCGAG 1 cut(s) 1519
PdiI GCCGGC 1 cut(s) 1326
PdmI GAANNNNTTC 3 cut(s) 1005, 1269, 1694
PfeI GAWTC 6 cut(s) 13, 498, 668, 897, 1001, 1270
PkrI GCNGC 2 cut(s) 328, 880
PleI GAGTC 1 cut(s) 1169
PpsI GAGTC 1 cut(s) 1169
Psp6I CCWGG 1 cut(s) 161
PspGI CCWGG 1 cut(s) 161
PspPI GGNCC 4 cut(s) 908, 1212, 1497, 1506
PspXI VCTCGAGB 1 cut(s) 1519
PstI CTGCAG 1 cut(s) 717
PsuI RGATCY 1 cut(s) 213
RigI GGCCGGCC 1 cut(s) 1328
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SaqAI TTAA 6 cut(s) 174, 420, 440, 585, 684, 1133
SatI GCNGC 2 cut(s) 327, 879
Sau3AI GATC 2 cut(s) 29, 213
Sau96I GGNCC 4 cut(s) 908, 1212, 1497, 1506
SchI GAGTC 1 cut(s) 1169
ScrFI CCNGG 1 cut(s) 163
SduI GDGCHC 2 cut(s) 948, 1436
SfaNI GCATC 5 cut(s) 44, 118, 466, 567, 1288
SfcI CTRYAG 1 cut(s) 713
Sfr274I CTCGAG 1 cut(s) 1519
SinI GGWCC 1 cut(s) 908
SlaI CTCGAG 1 cut(s) 1519
SmlI CTYRAG 5 cut(s) 419, 776, 1463, 1501, 1519
SmoI CTYRAG 5 cut(s) 419, 776, 1463, 1501, 1519
SspI AATATT 1 cut(s) 1090
SspMI CTAG 4 cut(s) 882, 894, 1302, 1316
StyD4I CCNGG 1 cut(s) 161
StyI CCWWGG 3 cut(s) 643, 1238, 1315
TaaI ACNGT 2 cut(s) 388, 1022
TaqI TCGA 2 cut(s) 1520, 1605
TatI WGTACW 1 cut(s) 255
TfiI GAWTC 6 cut(s) 13, 498, 668, 897, 1001, 1270
Tru1I TTAA 6 cut(s) 174, 420, 440, 585, 684, 1133
Tru9I TTAA 6 cut(s) 174, 420, 440, 585, 684, 1133
TscAI CASTG 3 cut(s) 15, 106, 708
TseFI GTSAC 4 cut(s) 590, 767, 794, 1022
TseI GCWGC 2 cut(s) 326, 878
Tsp45I GTSAC 4 cut(s) 590, 767, 794, 1022
TspGWI ACGGA 1 cut(s) 1548
TspRI CASTG 3 cut(s) 15, 106, 708
Vha464I CTTAAG 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 908
XapI RAATTY 8 cut(s) 4, 82, 581, 654, 675, 719, 1143, 1487
XceI RCATGY 1 cut(s) 1108
XcmI CCANNNNNNNNNTGG 3 cut(s) 232, 1245, 1681
XhoI CTCGAG 1 cut(s) 1519
XmaJI CCTAGG 1 cut(s) 1315
XmnI GAANNNNTTC 3 cut(s) 1005, 1269, 1694
XspI CTAG 4 cut(s) 882, 894, 1302, 1316
Zsp2I ATGCAT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.