MD03G1181900.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000229
Physical Location & Seq
Forward (+)
24889455 .. 24890135
681 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1181900.v1.1.491

Sequence Viewer

Length: 681 bp
ATGAGTTCTAGTTTTCGATCGAATTTATACCGAAAGACGAAAACTACTACAATTAAGGAGAGAAGTCGTTTCCTACTATCAAAGATGTTGTCGATGATACAATGCTTGTGTTATATCTATATGATGATTGTGGCTAATTACTTAACTGCAGGAGCATTAGCAATAGCTCATACCAATTTCAGCACAGATCAATCTGCGCTTCTTGCTCTCAAGGCTCACATCACTAGTGACCCTCAAAACATCTTGACCGCCAACTGGTCCTCTGCCTCCAATTCCAACATTTGCAACTGGGTTGGCGTTACCTGCGGTGCTCGCCACCACAGAGTCACGGCCTTAAATCTCCCTTACATGGGTCTTGCCGGAGTTATTCCTCCGCATCTTGGCAACCTCTCATTTCTCGTTGAGTTGGGCCTTCAGAATAATAGTTTTCATGGTCCCCTACCGCAAGAACTGTCTCGTTTGCGCCGGTTGAAGGCGATTAACTTTGGAAACAACAGCTTTATGGGAACCATTCCTTTGTGGTTTGGGTCCTTTGCTAAACTTCAAACCATCAAATTGTACGATAATGACTTCTCTGGTTTCATACCCGCTGCTATCTTCAACTTATCTGCACTCGAAAACATTTACCTGATGAGGAACCAACTATCGGGTACGTACATACCACCATTACCAACTACCTAG

Protein Analysis

227

Amino Acids

25.1

Weight (kDa)

9.73

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 62 - 102 1.6e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 155 - 215 2.8e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000229)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13780 FvH4_1g13790 FvH4_3g37750
malus_domestica MD00G1192800.v1.1 MD02G1291700.v1.1 MD03G1180500.v1.1 MD03G1181600.v1.1 MD03G1181700.v1.1 MD03G1181900.v1.1 MD03G1182700.v1.1 MD03G1281400.v1.1 MD05G1297800.v1.1 MD05G1298300.v1.1 MD07G1034800.v1.1 MD10G1265200.v1.1 MD10G1277500.v1.1
prunus_persica Prupe.4G063500_v2.0.a1 Prupe.6G160500_v2.0.a1
pyrus_communis pycom04g04980 pycom05g27570 pycom05g27590 pycom10g25090
rosa_chinensis RchiOBHm_Chr2g0113621 RchiOBHm_Chr2g0113671 RchiOBHm_Chr2g0113771 RchiOBHm_Chr2g0113871 RchiOBHm_Chr2g0114031 RchiOBHm_Chr2g0114181 RchiOBHm_Chr2g0114201 RchiOBHm_Chr2g0114231 RchiOBHm_Chr2g0114241 RchiOBHm_Chr4g0403181 RchiOBHm_Chr4g0404251 RchiOBHm_Chr4g0404291 RchiOBHm_Chr4g0404471 RchiOBHm_Chr5g0010961 RchiOBHm_Chr5g0010971 RchiOBHm_Chr5g0010981 RchiOBHm_Chr5g0011131 RchiOBHm_Chr5g0011311 RchiOBHm_Chr5g0011321 RchiOBHm_Chr5g0011361 RchiOBHm_Chr5g0016031 RchiOBHm_Chr5g0040141 RchiOBHm_Chr5g0040411 RchiOBHm_Chr5g0040471 RchiOBHm_Chr5g0059151 RchiOBHm_Chr5g0059191 RchiOBHm_Chr6g0251861 RchiOBHm_Chr7g0213311 RchiOBHm_Chr7g0213321
rosa_laevigata RLG00000009001 RLG00000009062 RLG00000018075 RLG00000018085 RLG00000018097 RLG00000031818 RLG00000034199
rosa_multiflora Rmu_co7972198.1_g000001 Rmu_co8328773.1_g000001 Rmu_sc0000929.1_g000033 Rmu_sc0001023.1_g000056 Rmu_sc0001699.1_g000007 Rmu_sc0003151.1_g000028 Rmu_sc0003242.1_g000010 Rmu_sc0004283.1_g000012 Rmu_sc0004283.1_g000036 Rmu_sc0004283.1_g000048 Rmu_sc0004283.1_g000066 Rmu_sc0005187.1_g000011 Rmu_sc0007010.1_g000008 Rmu_sc0007109.1_g000001 Rmu_sc0008397.1_g000007 Rmu_sc0009151.1_g000001 Rmu_sc0009639.1_g000020 Rmu_sc0009975.1_g000007 Rmu_sc0009975.1_g000010 Rmu_sc0010543.1_g000006 Rmu_sc0011398.1_g000001 Rmu_sc0011941.1_g000025 Rmu_sc0013141.1_g000002 Rmu_sc0016457.1_g000002 Rmu_sc0038501.1_g000001 Rmu_ssc0000303.1_g000008
rosa_roxburghii Rroxscaffold_1G00040180 Rroxscaffold_1G00040280 Rroxscaffold_1G00040900 Rroxscaffold_1G00064830 Rroxscaffold_1G00065410 Rroxscaffold_2G00130010 Rroxscaffold_2G00130040
rosa_rugosa Rorug02G0188500 Rorug02G0188800 Rorug02G0188900 Rorug02G0189500 Rorug04G0458400 Rorug05G0184800
rosa_samantha Rh1CG253300 Rh2CG247500 Rh2CG247600 Rh2CG247900 Rh2CG248300 Rh2CG249100 Rh2CG249400 Rh2CG249800 Rh2CG249900 Rh2CG250000 Rh2CG250100 Rh2CG250200 Rh2CG262700 Rh4AG118100 Rh4AG118300 Rh4AG364100 Rh4BG106900 Rh4CG125100 Rh4DG110100 Rh4DG110500 Rh4DG110600 Rh4DG369800 Rh5CG093500 Rh5CG093600 Rh5CG093700 Rh5CG095300 Rh5CG095500 Rh5CG096100 Rh5CG096200 Rh5CG096400 Rh5CG099600 Rh5CG134600 Rh5CG421300 Rh6DG142900 Rh7AG370200
rosa_wichuraiana Rw0G001470 Rw0G014660 Rw0G017510 Rw0G017750 Rw1G005500 Rw2G018440 Rw2G018460 Rw2G018490 Rw2G019150 Rw4G009550 Rw5G007590 Rw5G007860 Rw6G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 311
AciI CCGC 5 cut(s) 249, 306, 374, 443, 588
AcsI RAATTY 1 cut(s) 22
AcuI CTGAAG 1 cut(s) 398
AfaI GTAC 3 cut(s) 560, 652, 656
AfiI CCNNNNNNNGG 6 cut(s) 255, 349, 350, 380, 472, 646
AgsI TTSAA 3 cut(s) 472, 545, 601
AhlI ACTAGT 1 cut(s) 224
AluBI AGCT 2 cut(s) 167, 498
AluI AGCT 2 cut(s) 167, 498
Alw21I GWGCWC 1 cut(s) 313
Alw26I GTCTC 1 cut(s) 459
AoxI GGCC 2 cut(s) 330, 409
ApeKI GCWGC 1 cut(s) 590
ApoI RAATTY 1 cut(s) 22
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AspLEI GCGC 2 cut(s) 199, 465
AspS9I GGNCC 4 cut(s) 258, 409, 434, 528
AvaII GGWCC 3 cut(s) 258, 434, 528
Bbv12I GWGCWC 1 cut(s) 313
BbvI GCAGC 1 cut(s) 577
BccI CCATC 1 cut(s) 557
BceAI ACGGC 1 cut(s) 345
BcoDI GTCTC 1 cut(s) 459
BcuI ACTAGT 1 cut(s) 224
BfaI CTAG 3 cut(s) 9, 225, 679
BfmI CTRYAG 1 cut(s) 147
BfuAI ACCTGC 1 cut(s) 311
BisI GCNGC 1 cut(s) 591
BlsI GCNGC 1 cut(s) 592
Bme18I GGWCC 3 cut(s) 258, 434, 528
BmgT120I GGNCC 4 cut(s) 258, 409, 434, 528
BmiI GGNNCC 4 cut(s) 436, 508, 529, 638
BmrI ACTGGG 1 cut(s) 298
BmsI GCATC 1 cut(s) 385
BmuI ACTGGG 1 cut(s) 298
BpuEI CTTGAG 1 cut(s) 194
BsaAI YACGTR 1 cut(s) 654
Bsc4I CCNNNNNNNGG 6 cut(s) 255, 349, 350, 380, 472, 646
Bse118I RCCGGY 1 cut(s) 465
Bse1I ACTGG 2 cut(s) 260, 293
BseLI CCNNNNNNNGG 6 cut(s) 255, 349, 350, 380, 472, 646
BseNI ACTGG 2 cut(s) 260, 293
BseXI GCAGC 1 cut(s) 577
BsgI GTGCAG 1 cut(s) 594
Bsh1285I CGRYCG 1 cut(s) 20
BshFI GGCC 2 cut(s) 332, 411
BsiEI CGRYCG 1 cut(s) 20
BsiHKAI GWGCWC 1 cut(s) 313
BsiSI CCGG 2 cut(s) 360, 466
BslFI GGGAC 1 cut(s) 420
BslI CCNNNNNNNGG 6 cut(s) 255, 349, 350, 380, 472, 646
BsmAI GTCTC 1 cut(s) 459
BsmFI GGGAC 1 cut(s) 420
BsnI GGCC 2 cut(s) 332, 411
Bsp1286I GDGCHC 1 cut(s) 313
Bsp143I GATC 2 cut(s) 17, 187
BspACI CCGC 5 cut(s) 249, 306, 374, 443, 588
BspANI GGCC 2 cut(s) 332, 411
BspLI GGNNCC 4 cut(s) 436, 508, 529, 638
BspMAI CTGCAG 1 cut(s) 151
BspMI ACCTGC 1 cut(s) 311
BsrFI RCCGGY 1 cut(s) 465
BsrI ACTGG 2 cut(s) 260, 293
BssAI RCCGGY 1 cut(s) 465
BssMI GATC 2 cut(s) 17, 187
Bst4CI ACNGT 1 cut(s) 453
BstBAI YACGTR 1 cut(s) 654
BstC8I GCNNGC 1 cut(s) 313
BstHHI GCGC 2 cut(s) 199, 465
BstKTI GATC 2 cut(s) 20, 190
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 2 cut(s) 17, 187
BstMCI CGRYCG 1 cut(s) 20
BstMWI GCNNNNNNNGC 4 cut(s) 203, 212, 303, 312
BstSFI CTRYAG 1 cut(s) 147
BstSNI TACGTA 1 cut(s) 654
BstV1I GCAGC 1 cut(s) 577
BsuRI GGCC 2 cut(s) 332, 411
BveI ACCTGC 1 cut(s) 311
Cac8I GCNNGC 1 cut(s) 313
CfoI GCGC 2 cut(s) 199, 465
Cfr10I RCCGGY 1 cut(s) 465
Cfr13I GGNCC 4 cut(s) 258, 409, 434, 528
Csp6I GTAC 3 cut(s) 559, 651, 655
CviAII CATG 2 cut(s) 349, 431
CviJI RGCY 6 cut(s) 134, 167, 215, 332, 411, 498
CviKI_1 RGCY 6 cut(s) 134, 167, 215, 332, 411, 498
CviQI GTAC 3 cut(s) 559, 651, 655
DpnI GATC 2 cut(s) 19, 189
DpnII GATC 2 cut(s) 17, 187
Eco105I TACGTA 1 cut(s) 654
Eco47I GGWCC 3 cut(s) 258, 434, 528
Eco57I CTGAAG 1 cut(s) 398
EcoO109I RGGNCCY 1 cut(s) 528
FaeI CATG 2 cut(s) 352, 434
FaqI GGGAC 1 cut(s) 420
FatI CATG 2 cut(s) 348, 430
FauI CCCGC 1 cut(s) 595
Fnu4HI GCNGC 1 cut(s) 591
Fsp4HI GCNGC 1 cut(s) 591
FspBI CTAG 3 cut(s) 9, 225, 679
GlaI GCGC 2 cut(s) 198, 464
GluI GCNGC 1 cut(s) 591
HaeIII GGCC 2 cut(s) 332, 411
HapII CCGG 2 cut(s) 360, 466
HhaI GCGC 2 cut(s) 199, 465
Hin1II CATG 2 cut(s) 352, 434
Hin6I GCGC 2 cut(s) 197, 463
HinP1I GCGC 2 cut(s) 197, 463
HinfI GANTC 1 cut(s) 324
HpaII CCGG 2 cut(s) 360, 466
Hpy188I TCNGA 1 cut(s) 417
Hpy188III TCNNGA 1 cut(s) 244
HpyAV CCTTC 2 cut(s) 422, 466
HpyCH4III ACNGT 1 cut(s) 453
HpyCH4IV ACGT 1 cut(s) 653
HpyCH4V TGCA 3 cut(s) 149, 285, 611
HpyF10VI GCNNNNNNNGC 4 cut(s) 203, 212, 303, 312
HpySE526I ACGT 1 cut(s) 653
Hsp92II CATG 2 cut(s) 352, 434
HspAI GCGC 2 cut(s) 197, 463
Kzo9I GATC 2 cut(s) 17, 187
LmnI GCTCC 1 cut(s) 152
LpnPI CCDG 8 cut(s) 135, 241, 274, 316, 373, 479, 561, 641
Lsp1109I GCAGC 1 cut(s) 577
LweI GCATC 1 cut(s) 385
MaeI CTAG 3 cut(s) 9, 225, 679
MaeII ACGT 1 cut(s) 653
MaeIII GTNAC 3 cut(s) 227, 298, 325
MalI GATC 2 cut(s) 19, 189
MboI GATC 2 cut(s) 17, 187
MboII GAAGA 1 cut(s) 589
MhlI GDGCHC 1 cut(s) 313
MluCI AATT 6 cut(s) 22, 51, 136, 175, 271, 554
MlyI GAGTC 1 cut(s) 333
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 6 cut(s) 243, 271, 277, 381, 398, 627
MseI TTAA 4 cut(s) 54, 143, 335, 480
MspA1I CMGCKG 1 cut(s) 590
MspI CCGG 2 cut(s) 360, 466
MwoI GCNNNNNNNGC 4 cut(s) 203, 212, 303, 312
NdeII GATC 2 cut(s) 17, 187
NlaIII CATG 2 cut(s) 352, 434
NlaIV GGNNCC 4 cut(s) 436, 508, 529, 638
NmuCI GTSAC 2 cut(s) 227, 325
PkrI GCNGC 1 cut(s) 592
Ple19I CGATCG 1 cut(s) 20
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
Ppu21I YACGTR 1 cut(s) 654
PpuMI RGGWCCY 1 cut(s) 528
Psp5II RGGWCCY 1 cut(s) 528
PspN4I GGNNCC 4 cut(s) 436, 508, 529, 638
PspPI GGNCC 4 cut(s) 258, 409, 434, 528
PspPPI RGGWCCY 1 cut(s) 528
PstI CTGCAG 1 cut(s) 151
PvuI CGATCG 1 cut(s) 20
RsaI GTAC 3 cut(s) 560, 652, 656
RsaNI GTAC 3 cut(s) 559, 651, 655
SaqAI TTAA 4 cut(s) 54, 143, 335, 480
SatI GCNGC 1 cut(s) 591
Sau3AI GATC 2 cut(s) 17, 187
Sau96I GGNCC 4 cut(s) 258, 409, 434, 528
SchI GAGTC 1 cut(s) 333
SduI GDGCHC 1 cut(s) 313
SetI ASST 7 cut(s) 169, 305, 390, 500, 630, 656, 680
SfaNI GCATC 1 cut(s) 385
SfcI CTRYAG 1 cut(s) 147
SinI GGWCC 3 cut(s) 258, 434, 528
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
SnaBI TACGTA 1 cut(s) 654
SpeI ACTAGT 1 cut(s) 224
Sse9I AATT 6 cut(s) 22, 51, 136, 175, 271, 554
SsiI CCGC 5 cut(s) 249, 306, 374, 443, 588
SspMI CTAG 3 cut(s) 9, 225, 679
TaaI ACNGT 1 cut(s) 453
TaiI ACGT 1 cut(s) 656
TaqI TCGA 4 cut(s) 16, 20, 92, 615
TasI AATT 6 cut(s) 22, 51, 136, 175, 271, 554
Tru1I TTAA 4 cut(s) 54, 143, 335, 480
Tru9I TTAA 4 cut(s) 54, 143, 335, 480
TseFI GTSAC 2 cut(s) 227, 325
TseI GCWGC 1 cut(s) 590
Tsp45I GTSAC 2 cut(s) 227, 325
TspDTI ATGAA 2 cut(s) 419, 571
VpaK11BI GGWCC 3 cut(s) 258, 434, 528
XapI RAATTY 1 cut(s) 22
XspI CTAG 3 cut(s) 9, 225, 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.