Rroxscaffold_1G00040180

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000229
Physical Location & Seq
Reverse (-)
57783878 .. 57786723
2846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00040180.1

Sequence Viewer

Length: 918 bp
ATGCAAGCAATGTTGAGAAATCGGATCCTATTGTCAATAGTGTTGTTGCTGATACAACACTGGTCCACAGCAGCACAAACGAGCATCAGCACAGACCATTCTGCTCTTCTTGCTCTCAAATCTCATATCACCAATGACCCTCAAAACATGATCTTGACCAACTGGTCAACCCCCAGCTCCATTTGCAACTGGATTGGAGTTACTTGTGGTGCACGGCATCTTCGAGTTACTGCCTTGAATCTCTCTTACATGGATGTCACAGGCACCATTCCTCCGCAGCTAGGCAACCTATCATTTCTTGTTGAGCTGCGCTTCAGAAATAACAGTTTCTATGGTACCATTCCCCAAGAATTGTCTCGTTTGCGCCGCCTGAAGATTCTTAATTTCGGATTCAACAACTTCAAAGGAACCATTCCCTCATGGTTGGGAGCCGTATCCAAACTTCAAATCGTCAGTTTGTATGGTAATCAGTTTTCAGGTTTAATACCTACCAACATCTTCAACTTATCTGCAGTGCAACAAATTGATCTTAGGTTCAATGAACTATCAGAATTTCCAAATGAAATTGGCACATTAGACCAGCTGGAAGAGATAAGTGTACAAGACAATGCCCTAGAAGGGCATCTTCCTGGGGCTATCTTCAACATGTCTTCCTTGACTGCATTGGCTCTGACAGGAAACAGCTTGAATGGTAGACTTCCAGACAATATATGTCAGCATCTTCCTAGTATTCAAAAGTTGTATTTGTCACACAACCAGTTTGTCGGTCCTCTTCCATCCAAATTATGGCAATGCAAAGAGCTTGCGGAATTAGCATGGGGGTATAATAATTTCAGTGGAAGCATACCCAAAAATATAGGGAATTTGACCCAGATAAAGTATATTCATCTCGGCTACAACAATTTGACAGGTACCTAG

Protein Analysis

305

Amino Acids

33.93

Weight (kDa)

8.68

Isoelectric Point (pI)

29.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 69 8.1e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 145 - 224 7.5e-06 Leucine-rich repeat region
LRR_8 PF13855 194 - 254 4.5e-09 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000229)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13780 FvH4_1g13790 FvH4_3g37750
malus_domestica MD00G1192800.v1.1 MD02G1291700.v1.1 MD03G1180500.v1.1 MD03G1181600.v1.1 MD03G1181700.v1.1 MD03G1181900.v1.1 MD03G1182700.v1.1 MD03G1281400.v1.1 MD05G1297800.v1.1 MD05G1298300.v1.1 MD07G1034800.v1.1 MD10G1265200.v1.1 MD10G1277500.v1.1
prunus_persica Prupe.4G063500_v2.0.a1 Prupe.6G160500_v2.0.a1
pyrus_communis pycom04g04980 pycom05g27570 pycom05g27590 pycom10g25090
rosa_chinensis RchiOBHm_Chr2g0113621 RchiOBHm_Chr2g0113671 RchiOBHm_Chr2g0113771 RchiOBHm_Chr2g0113871 RchiOBHm_Chr2g0114031 RchiOBHm_Chr2g0114181 RchiOBHm_Chr2g0114201 RchiOBHm_Chr2g0114231 RchiOBHm_Chr2g0114241 RchiOBHm_Chr4g0403181 RchiOBHm_Chr4g0404251 RchiOBHm_Chr4g0404291 RchiOBHm_Chr4g0404471 RchiOBHm_Chr5g0010961 RchiOBHm_Chr5g0010971 RchiOBHm_Chr5g0010981 RchiOBHm_Chr5g0011131 RchiOBHm_Chr5g0011311 RchiOBHm_Chr5g0011321 RchiOBHm_Chr5g0011361 RchiOBHm_Chr5g0016031 RchiOBHm_Chr5g0040141 RchiOBHm_Chr5g0040411 RchiOBHm_Chr5g0040471 RchiOBHm_Chr5g0059151 RchiOBHm_Chr5g0059191 RchiOBHm_Chr6g0251861 RchiOBHm_Chr7g0213311 RchiOBHm_Chr7g0213321
rosa_laevigata RLG00000009001 RLG00000009062 RLG00000018075 RLG00000018085 RLG00000018097 RLG00000031818 RLG00000034199
rosa_multiflora Rmu_co7972198.1_g000001 Rmu_co8328773.1_g000001 Rmu_sc0000929.1_g000033 Rmu_sc0001023.1_g000056 Rmu_sc0001699.1_g000007 Rmu_sc0003151.1_g000028 Rmu_sc0003242.1_g000010 Rmu_sc0004283.1_g000012 Rmu_sc0004283.1_g000036 Rmu_sc0004283.1_g000048 Rmu_sc0004283.1_g000066 Rmu_sc0005187.1_g000011 Rmu_sc0007010.1_g000008 Rmu_sc0007109.1_g000001 Rmu_sc0008397.1_g000007 Rmu_sc0009151.1_g000001 Rmu_sc0009639.1_g000020 Rmu_sc0009975.1_g000007 Rmu_sc0009975.1_g000010 Rmu_sc0010543.1_g000006 Rmu_sc0011398.1_g000001 Rmu_sc0011941.1_g000025 Rmu_sc0013141.1_g000002 Rmu_sc0016457.1_g000002 Rmu_sc0038501.1_g000001 Rmu_ssc0000303.1_g000008
rosa_roxburghii Rroxscaffold_1G00040180 Rroxscaffold_1G00040280 Rroxscaffold_1G00040900 Rroxscaffold_1G00064830 Rroxscaffold_1G00065410 Rroxscaffold_2G00130010 Rroxscaffold_2G00130040
rosa_rugosa Rorug02G0188500 Rorug02G0188800 Rorug02G0188900 Rorug02G0189500 Rorug04G0458400 Rorug05G0184800
rosa_samantha Rh1CG253300 Rh2CG247500 Rh2CG247600 Rh2CG247900 Rh2CG248300 Rh2CG249100 Rh2CG249400 Rh2CG249800 Rh2CG249900 Rh2CG250000 Rh2CG250100 Rh2CG250200 Rh2CG262700 Rh4AG118100 Rh4AG118300 Rh4AG364100 Rh4BG106900 Rh4CG125100 Rh4DG110100 Rh4DG110500 Rh4DG110600 Rh4DG369800 Rh5CG093500 Rh5CG093600 Rh5CG093700 Rh5CG095300 Rh5CG095500 Rh5CG096100 Rh5CG096200 Rh5CG096400 Rh5CG099600 Rh5CG134600 Rh5CG421300 Rh6DG142900 Rh7AG370200
rosa_wichuraiana Rw0G001470 Rw0G014660 Rw0G017510 Rw0G017750 Rw1G005500 Rw2G018440 Rw2G018460 Rw2G018490 Rw2G019150 Rw4G009550 Rw5G007590 Rw5G007860 Rw6G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 163
Acc65I GGTACC 2 cut(s) 335, 911
AccB1I GGYRCC 3 cut(s) 263, 335, 911
AccB7I CCANNNNNTGG 1 cut(s) 786
AccI GTMKAC 1 cut(s) 694
AciI CCGC 3 cut(s) 275, 367, 806
AclWI GGATC 2 cut(s) 19, 32
AcsI RAATTY 2 cut(s) 551, 862
AcuI CTGAAG 2 cut(s) 298, 392
AfaI GTAC 3 cut(s) 337, 600, 913
AfiI CCNNNNNNNGG 3 cut(s) 281, 618, 786
AflIII ACRYGT 1 cut(s) 645
AgsI TTSAA 9 cut(s) 238, 394, 403, 446, 502, 538, 643, 688, 734
AjnI CCWGG 1 cut(s) 628
AluBI AGCT 6 cut(s) 177, 280, 307, 583, 684, 802
AluI AGCT 6 cut(s) 177, 280, 307, 583, 684, 802
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 1 cut(s) 360
Alw44I GTGCAC 1 cut(s) 210
AlwI GGATC 2 cut(s) 19, 32
ApaLI GTGCAC 1 cut(s) 210
ApeKI GCWGC 3 cut(s) 71, 277, 307
ApoI RAATTY 2 cut(s) 551, 862
Asp718I GGTACC 2 cut(s) 335, 911
AspLEI GCGC 2 cut(s) 312, 366
AspS9I GGNCC 2 cut(s) 63, 767
AsuHPI GGTGA 1 cut(s) 121
AvaII GGWCC 2 cut(s) 63, 767
BaeGI GKGCMC 1 cut(s) 214
BamHI GGATCC 1 cut(s) 24
BanI GGYRCC 3 cut(s) 263, 335, 911
BarI GAAGNNNNNNTAC 2 cut(s) 426, 458
BbsI GAAGAC 1 cut(s) 642
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 3 cut(s) 83, 289, 294
BccI CCATC 1 cut(s) 784
BceAI ACGGC 2 cut(s) 230, 416
BciT130I CCWGG 1 cut(s) 630
BciVI GTATCC 1 cut(s) 445
BcoDI GTCTC 1 cut(s) 360
BfaI CTAG 4 cut(s) 281, 614, 726, 916
BfmI CTRYAG 1 cut(s) 510
BfuI GTATCC 1 cut(s) 445
BisI GCNGC 4 cut(s) 72, 278, 308, 367
BlsI GCNGC 4 cut(s) 73, 279, 309, 368
Bme1390I CCNGG 1 cut(s) 630
Bme18I GGWCC 2 cut(s) 63, 767
BmgT120I GGNCC 2 cut(s) 63, 767
BmiI GGNNCC 6 cut(s) 26, 265, 337, 409, 430, 913
BmrFI CCNGG 1 cut(s) 630
BmsI GCATC 4 cut(s) 93, 226, 631, 727
BpiI GAAGAC 1 cut(s) 642
BsaJI CCNNGG 1 cut(s) 629
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 618, 786
Bse1I ACTGG 4 cut(s) 65, 167, 194, 757
Bse3DI GCAATG 2 cut(s) 15, 797
BseBI CCWGG 1 cut(s) 630
BseDI CCNNGG 1 cut(s) 629
BseGI GGATG 2 cut(s) 259, 776
BseLI CCNNNNNNNGG 3 cut(s) 281, 618, 786
BseMI GCAATG 2 cut(s) 15, 797
BseNI ACTGG 4 cut(s) 65, 167, 194, 757
BseSI GKGCMC 1 cut(s) 214
BseXI GCAGC 3 cut(s) 83, 289, 294
BseYI CCCAGC 1 cut(s) 173
BshNI GGYRCC 3 cut(s) 263, 335, 911
BsiHKAI GWGCWC 1 cut(s) 214
BslI CCNNNNNNNGG 3 cut(s) 281, 618, 786
BsmAI GTCTC 1 cut(s) 360
Bsp1286I GDGCHC 1 cut(s) 214
Bsp1407I TGTACA 1 cut(s) 598
Bsp143I GATC 3 cut(s) 24, 150, 526
BspACI CCGC 3 cut(s) 275, 367, 806
BspLI GGNNCC 6 cut(s) 26, 265, 337, 409, 430, 913
BspMAI CTGCAG 1 cut(s) 514
BspPI GGATC 2 cut(s) 19, 32
BspQI GCTCTTC 1 cut(s) 111
BspT107I GGYRCC 3 cut(s) 263, 335, 911
BsrDI GCAATG 2 cut(s) 15, 797
BsrGI TGTACA 1 cut(s) 598
BsrI ACTGG 4 cut(s) 65, 167, 194, 757
BssECI CCNNGG 1 cut(s) 629
BssMI GATC 3 cut(s) 24, 150, 526
Bst2UI CCWGG 1 cut(s) 630
Bst4CI ACNGT 1 cut(s) 326
Bst6I CTCTTC 3 cut(s) 111, 582, 777
BstAUI TGTACA 1 cut(s) 598
BstC8I GCNNGC 2 cut(s) 6, 804
BstDEI CTNAG 1 cut(s) 530
BstF5I GGATG 2 cut(s) 259, 776
BstHHI GCGC 2 cut(s) 312, 366
BstKTI GATC 3 cut(s) 27, 153, 529
BstMAI GTCTC 1 cut(s) 360
BstMBI GATC 3 cut(s) 24, 150, 526
BstMWI GCNNNNNNNGC 3 cut(s) 110, 183, 812
BstNI CCWGG 1 cut(s) 630
BstNSI RCATGY 1 cut(s) 649
BstSCI CCNGG 1 cut(s) 628
BstSFI CTRYAG 1 cut(s) 510
BstSLI GKGCMC 1 cut(s) 214
BstV1I GCAGC 3 cut(s) 83, 289, 294
BstV2I GAAGAC 1 cut(s) 642
BstX2I RGATCY 1 cut(s) 24
BstYI RGATCY 1 cut(s) 24
BsuI GTATCC 1 cut(s) 445
BtsCI GGATG 2 cut(s) 259, 776
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 3 cut(s) 58, 519, 841
Cac8I GCNNGC 2 cut(s) 6, 804
CfoI GCGC 2 cut(s) 312, 366
Cfr13I GGNCC 2 cut(s) 63, 767
Csp6I GTAC 3 cut(s) 336, 599, 912
CviAII CATG 5 cut(s) 148, 250, 420, 646, 816
CviQI GTAC 3 cut(s) 336, 599, 912
DdeI CTNAG 1 cut(s) 530
DpnI GATC 3 cut(s) 26, 152, 528
DpnII GATC 3 cut(s) 24, 150, 526
DrdI GACNNNNNNGTC 1 cut(s) 163
DseDI GACNNNNNNGTC 1 cut(s) 163
Eam1104I CTCTTC 3 cut(s) 111, 582, 777
EarI CTCTTC 3 cut(s) 111, 582, 777
Eco47I GGWCC 2 cut(s) 63, 767
Eco57I CTGAAG 2 cut(s) 298, 392
EcoRII CCWGG 1 cut(s) 628
FaeI CATG 5 cut(s) 151, 253, 423, 649, 819
FalI AAGNNNNNCTT 2 cut(s) 609, 641
FatI CATG 5 cut(s) 147, 249, 419, 645, 815
FblI GTMKAC 1 cut(s) 694
Fnu4HI GCNGC 4 cut(s) 72, 278, 308, 367
FokI GGATG 2 cut(s) 266, 763
Fsp4HI GCNGC 4 cut(s) 72, 278, 308, 367
FspBI CTAG 4 cut(s) 281, 614, 726, 916
GlaI GCGC 2 cut(s) 311, 365
GluI GCNGC 4 cut(s) 72, 278, 308, 367
GsaI CCCAGC 1 cut(s) 177
HhaI GCGC 2 cut(s) 312, 366
Hin1II CATG 5 cut(s) 151, 253, 423, 649, 819
Hin6I GCGC 2 cut(s) 310, 364
HinP1I GCGC 2 cut(s) 310, 364
HincII GTYRAC 1 cut(s) 168
HindII GTYRAC 1 cut(s) 168
HinfI GANTC 3 cut(s) 238, 376, 390
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 5 cut(s) 66, 168, 212, 599, 695
Hpy188I TCNGA 5 cut(s) 24, 317, 389, 550, 672
Hpy188III TCNNGA 2 cut(s) 154, 701
Hpy8I GTNNAC 5 cut(s) 66, 168, 212, 599, 695
HpyAV CCTTC 1 cut(s) 611
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4V TGCA 7 cut(s) 4, 186, 212, 512, 517, 662, 795
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 183, 812
HpyF3I CTNAG 1 cut(s) 530
Hsp92II CATG 5 cut(s) 151, 253, 423, 649, 819
HspAI GCGC 2 cut(s) 310, 364
KpnI GGTACC 2 cut(s) 339, 915
Kzo9I GATC 3 cut(s) 24, 150, 526
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 2 cut(s) 182, 428
Lsp1109I GCAGC 3 cut(s) 83, 289, 294
LweI GCATC 4 cut(s) 93, 226, 631, 727
MaeI CTAG 4 cut(s) 281, 614, 726, 916
MaeIII GTNAC 4 cut(s) 199, 226, 256, 747
MalI GATC 3 cut(s) 26, 152, 528
MboI GATC 3 cut(s) 24, 150, 526
MflI RGATCY 1 cut(s) 24
MhlI GDGCHC 1 cut(s) 214
MnlI CCTC 4 cut(s) 150, 282, 427, 780
MseI TTAA 2 cut(s) 381, 482
MspA1I CMGCKG 1 cut(s) 583
MspR9I CCNGG 1 cut(s) 630
MvaI CCWGG 1 cut(s) 630
MwoI GCNNNNNNNGC 3 cut(s) 110, 183, 812
NdeII GATC 3 cut(s) 24, 150, 526
NlaIII CATG 5 cut(s) 151, 253, 423, 649, 819
NlaIV GGNNCC 6 cut(s) 26, 265, 337, 409, 430, 913
NmeAIII GCCGAG 1 cut(s) 870
NmuCI GTSAC 2 cut(s) 256, 747
NspI RCATGY 1 cut(s) 649
PciI ACATGT 1 cut(s) 645
PciSI GCTCTTC 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 220
PfeI GAWTC 3 cut(s) 238, 376, 390
PflMI CCANNNNNTGG 1 cut(s) 786
PkrI GCNGC 4 cut(s) 73, 279, 309, 368
PscI ACATGT 1 cut(s) 645
Psp6I CCWGG 1 cut(s) 628
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 628
PspN4I GGNNCC 6 cut(s) 26, 265, 337, 409, 430, 913
PspPI GGNCC 2 cut(s) 63, 767
PstI CTGCAG 1 cut(s) 514
PsuI RGATCY 1 cut(s) 24
PvuII CAGCTG 1 cut(s) 583
RsaI GTAC 3 cut(s) 337, 600, 913
RsaNI GTAC 3 cut(s) 336, 599, 912
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 2 cut(s) 381, 482
SatI GCNGC 4 cut(s) 72, 278, 308, 367
Sau3AI GATC 3 cut(s) 24, 150, 526
Sau96I GGNCC 2 cut(s) 63, 767
ScrFI CCNGG 1 cut(s) 630
SduI GDGCHC 1 cut(s) 214
SfaNI GCATC 4 cut(s) 93, 226, 631, 727
SfcI CTRYAG 1 cut(s) 510
SinI GGWCC 2 cut(s) 63, 767
SsiI CCGC 3 cut(s) 275, 367, 806
SspMI CTAG 4 cut(s) 281, 614, 726, 916
StyD4I CCNGG 1 cut(s) 628
TaaI ACNGT 1 cut(s) 326
TaqI TCGA 1 cut(s) 223
TaqII GACCGA 1 cut(s) 755
TatI WGTACW 1 cut(s) 598
TauI GCSGC 1 cut(s) 369
TfiI GAWTC 3 cut(s) 238, 376, 390
Tru1I TTAA 2 cut(s) 381, 482
Tru9I TTAA 2 cut(s) 381, 482
TscAI CASTG 3 cut(s) 65, 519, 841
TseFI GTSAC 2 cut(s) 256, 747
TseI GCWGC 3 cut(s) 71, 277, 307
Tsp45I GTSAC 2 cut(s) 256, 747
TspDTI ATGAA 3 cut(s) 555, 576, 875
TspRI CASTG 3 cut(s) 65, 519, 841
Van91I CCANNNNNTGG 1 cut(s) 786
VneI GTGCAC 1 cut(s) 210
VpaK11BI GGWCC 2 cut(s) 63, 767
XapI RAATTY 2 cut(s) 551, 862
XceI RCATGY 1 cut(s) 649
XcmI CCANNNNNNNNNTGG 1 cut(s) 783
XmiI GTMKAC 1 cut(s) 694
XspI CTAG 4 cut(s) 281, 614, 726, 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.