MD07G1034800.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000229
Physical Location & Seq
Forward (+)
2849054 .. 2849761
708 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1034800.v1.1.491

Sequence Viewer

Length: 708 bp
ATGGCGAGAACCCGTTTCCTCTTGTCGACAACTTTGTTGTTGCTGCTATTCCAATACTGTTGTGTATACATACCTATCTTAATAACGGGTTCCGTAGCAGCACAAACCAACATCAGCACAGATCAGTCTGCTCTTCTTGCCCTCAAATCCCATATCACCATTGATCCTCAAAACATCTTGACCACCAACTGGTCTACCTCCGATTCCGACATTTGCAACTGGGTTGGCGTTACTTGCGGTGCACGCCACCTTAGAGTGACAACCTTGAATCTCTCCTACATGGGTCTCACTGGCACCATCCCTTCCCACCTAGGCAACCTCTCTTTTCTTGTTCAATTGGAATTCAGAAACAACAGTTTTGGTGGTACCTTGCCCCCGGAATTGTCTAATCTACGCAGGCTGAAGTTGATTAGCTTTGAATTCAATGACTTTGAAGGAACCATTCCATCATGGTTCGGGTTGTTATCTAAACTTCAAACCTTCAATTTGTACGGTAATCAATTTTCAGGTTCCATACCAAACGCCATCTTCAACTTATCTGCACTTCAAGTACTTAATCTAAGAAGCAACCAGCTATCCGGTACGTACATACGAACCAATTACCATACAAGCTATCAGGTTCCATACCATGTTATTTATCTGCTAGTTTTTACTTTTTTCCTTATTCCTTTTCTGTGGCCAATACATATTACAAACCCTATACTTTGA

Protein Analysis

236

Amino Acids

26.48

Weight (kDa)

8.73

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 39 - 79 2.7e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 89 - 191 2.9e-08 Leucine-rich repeat region
LRR_8 PF13855 133 - 192 3.2e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000229)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13780 FvH4_1g13790 FvH4_3g37750
malus_domestica MD00G1192800.v1.1 MD02G1291700.v1.1 MD03G1180500.v1.1 MD03G1181600.v1.1 MD03G1181700.v1.1 MD03G1181900.v1.1 MD03G1182700.v1.1 MD03G1281400.v1.1 MD05G1297800.v1.1 MD05G1298300.v1.1 MD07G1034800.v1.1 MD10G1265200.v1.1 MD10G1277500.v1.1
prunus_persica Prupe.4G063500_v2.0.a1 Prupe.6G160500_v2.0.a1
pyrus_communis pycom04g04980 pycom05g27570 pycom05g27590 pycom10g25090
rosa_chinensis RchiOBHm_Chr2g0113621 RchiOBHm_Chr2g0113671 RchiOBHm_Chr2g0113771 RchiOBHm_Chr2g0113871 RchiOBHm_Chr2g0114031 RchiOBHm_Chr2g0114181 RchiOBHm_Chr2g0114201 RchiOBHm_Chr2g0114231 RchiOBHm_Chr2g0114241 RchiOBHm_Chr4g0403181 RchiOBHm_Chr4g0404251 RchiOBHm_Chr4g0404291 RchiOBHm_Chr4g0404471 RchiOBHm_Chr5g0010961 RchiOBHm_Chr5g0010971 RchiOBHm_Chr5g0010981 RchiOBHm_Chr5g0011131 RchiOBHm_Chr5g0011311 RchiOBHm_Chr5g0011321 RchiOBHm_Chr5g0011361 RchiOBHm_Chr5g0016031 RchiOBHm_Chr5g0040141 RchiOBHm_Chr5g0040411 RchiOBHm_Chr5g0040471 RchiOBHm_Chr5g0059151 RchiOBHm_Chr5g0059191 RchiOBHm_Chr6g0251861 RchiOBHm_Chr7g0213311 RchiOBHm_Chr7g0213321
rosa_laevigata RLG00000009001 RLG00000009062 RLG00000018075 RLG00000018085 RLG00000018097 RLG00000031818 RLG00000034199
rosa_multiflora Rmu_co7972198.1_g000001 Rmu_co8328773.1_g000001 Rmu_sc0000929.1_g000033 Rmu_sc0001023.1_g000056 Rmu_sc0001699.1_g000007 Rmu_sc0003151.1_g000028 Rmu_sc0003242.1_g000010 Rmu_sc0004283.1_g000012 Rmu_sc0004283.1_g000036 Rmu_sc0004283.1_g000048 Rmu_sc0004283.1_g000066 Rmu_sc0005187.1_g000011 Rmu_sc0007010.1_g000008 Rmu_sc0007109.1_g000001 Rmu_sc0008397.1_g000007 Rmu_sc0009151.1_g000001 Rmu_sc0009639.1_g000020 Rmu_sc0009975.1_g000007 Rmu_sc0009975.1_g000010 Rmu_sc0010543.1_g000006 Rmu_sc0011398.1_g000001 Rmu_sc0011941.1_g000025 Rmu_sc0013141.1_g000002 Rmu_sc0016457.1_g000002 Rmu_sc0038501.1_g000001 Rmu_ssc0000303.1_g000008
rosa_roxburghii Rroxscaffold_1G00040180 Rroxscaffold_1G00040280 Rroxscaffold_1G00040900 Rroxscaffold_1G00064830 Rroxscaffold_1G00065410 Rroxscaffold_2G00130010 Rroxscaffold_2G00130040
rosa_rugosa Rorug02G0188500 Rorug02G0188800 Rorug02G0188900 Rorug02G0189500 Rorug04G0458400 Rorug05G0184800
rosa_samantha Rh1CG253300 Rh2CG247500 Rh2CG247600 Rh2CG247900 Rh2CG248300 Rh2CG249100 Rh2CG249400 Rh2CG249800 Rh2CG249900 Rh2CG250000 Rh2CG250100 Rh2CG250200 Rh2CG262700 Rh4AG118100 Rh4AG118300 Rh4AG364100 Rh4BG106900 Rh4CG125100 Rh4DG110100 Rh4DG110500 Rh4DG110600 Rh4DG369800 Rh5CG093500 Rh5CG093600 Rh5CG093700 Rh5CG095300 Rh5CG095500 Rh5CG096100 Rh5CG096200 Rh5CG096400 Rh5CG099600 Rh5CG134600 Rh5CG421300 Rh6DG142900 Rh7AG370200
rosa_wichuraiana Rw0G001470 Rw0G014660 Rw0G017510 Rw0G017750 Rw1G005500 Rw2G018440 Rw2G018460 Rw2G018490 Rw2G019150 Rw4G009550 Rw5G007590 Rw5G007860 Rw6G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 365
AccB1I GGYRCC 2 cut(s) 293, 365
AccB7I CCANNNNNTGG 1 cut(s) 189
AccI GTMKAC 3 cut(s) 26, 66, 194
AciI CCGC 1 cut(s) 237
AclWI GGATC 1 cut(s) 158
AcoI YGGCCR 1 cut(s) 677
AcsI RAATTY 2 cut(s) 341, 419
AcuI CTGAAG 1 cut(s) 422
AfaI GTAC 5 cut(s) 367, 491, 552, 583, 587
AfiI CCNNNNNNNGG 1 cut(s) 189
AgsI TTSAA 9 cut(s) 268, 335, 419, 424, 434, 476, 484, 532, 548
AluBI AGCT 3 cut(s) 414, 574, 612
AluI AGCT 3 cut(s) 414, 574, 612
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 1 cut(s) 290
Alw44I GTGCAC 1 cut(s) 240
AlwI GGATC 1 cut(s) 158
AoxI GGCC 1 cut(s) 677
ApaLI GTGCAC 1 cut(s) 240
ApeKI GCWGC 2 cut(s) 43, 98
ApoI RAATTY 2 cut(s) 341, 419
Asp718I GGTACC 1 cut(s) 365
AspA2I CCTAGG 1 cut(s) 310
AsuC2I CCSGG 1 cut(s) 377
AsuHPI GGTGA 1 cut(s) 148
AvrII CCTAGG 1 cut(s) 310
BaeGI GKGCMC 1 cut(s) 244
BalI TGGCCA 1 cut(s) 679
BanI GGYRCC 2 cut(s) 293, 365
Bbv12I GWGCWC 1 cut(s) 244
BbvI GCAGC 2 cut(s) 30, 110
BccI CCATC 3 cut(s) 305, 454, 533
BcnI CCSGG 1 cut(s) 377
BcoDI GTCTC 1 cut(s) 290
BfaI CTAG 2 cut(s) 311, 644
BisI GCNGC 2 cut(s) 44, 99
BlnI CCTAGG 1 cut(s) 310
BlsI GCNGC 2 cut(s) 45, 100
BmcAI AGTACT 1 cut(s) 552
Bme1390I CCNGG 1 cut(s) 377
BmiI GGNNCC 6 cut(s) 91, 295, 367, 439, 511, 621
BmrFI CCNGG 1 cut(s) 377
BmrI ACTGGG 1 cut(s) 229
BmuI ACTGGG 1 cut(s) 229
BpuMI CCSGG 1 cut(s) 377
BsaAI YACGTR 1 cut(s) 585
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 2 cut(s) 310, 375
BsaWI WCCGGW 1 cut(s) 578
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse1I ACTGG 3 cut(s) 194, 224, 295
BseDI CCNNGG 2 cut(s) 310, 375
BseGI GGATG 1 cut(s) 297
BseLI CCNNNNNNNGG 1 cut(s) 189
BseNI ACTGG 3 cut(s) 194, 224, 295
BseSI GKGCMC 1 cut(s) 244
BseXI GCAGC 2 cut(s) 30, 110
BsgI GTGCAG 1 cut(s) 525
BshFI GGCC 1 cut(s) 679
BshNI GGYRCC 2 cut(s) 293, 365
BsiHKAI GWGCWC 1 cut(s) 244
BsiSI CCGG 2 cut(s) 377, 579
BslI CCNNNNNNNGG 1 cut(s) 189
BsmAI GTCTC 1 cut(s) 290
BsnI GGCC 1 cut(s) 679
Bso31I GGTCTC 1 cut(s) 290
Bsp1286I GDGCHC 1 cut(s) 244
Bsp143I GATC 2 cut(s) 121, 163
BspACI CCGC 1 cut(s) 237
BspANI GGCC 1 cut(s) 679
BspLI GGNNCC 6 cut(s) 91, 295, 367, 439, 511, 621
BspPI GGATC 1 cut(s) 158
BspQI GCTCTTC 1 cut(s) 138
BspT107I GGYRCC 2 cut(s) 293, 365
BspTNI GGTCTC 1 cut(s) 290
BsrI ACTGG 3 cut(s) 194, 224, 295
BssECI CCNNGG 2 cut(s) 310, 375
BssMI GATC 2 cut(s) 121, 163
BssNAI GTATAC 1 cut(s) 67
BssT1I CCWWGG 1 cut(s) 310
Bst1107I GTATAC 1 cut(s) 67
Bst4CI ACNGT 3 cut(s) 59, 356, 494
Bst6I CTCTTC 1 cut(s) 138
BstBAI YACGTR 1 cut(s) 585
BstC8I GCNNGC 2 cut(s) 244, 398
BstDEI CTNAG 2 cut(s) 251, 560
BstF5I GGATG 1 cut(s) 297
BstKTI GATC 2 cut(s) 124, 166
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 2 cut(s) 121, 163
BstMWI GCNNNNNNNGC 3 cut(s) 137, 234, 243
BstSCI CCNGG 1 cut(s) 375
BstSLI GKGCMC 1 cut(s) 244
BstSNI TACGTA 1 cut(s) 585
BstV1I GCAGC 2 cut(s) 30, 110
BstZ17I GTATAC 1 cut(s) 67
BsuRI GGCC 1 cut(s) 679
BtsCI GGATG 1 cut(s) 297
BtsIMutI CAGTG 1 cut(s) 288
Cac8I GCNNGC 2 cut(s) 244, 398
Csp6I GTAC 5 cut(s) 366, 490, 551, 582, 586
CviAII CATG 3 cut(s) 280, 450, 629
CviJI RGCY 5 cut(s) 400, 414, 574, 612, 679
CviKI_1 RGCY 5 cut(s) 400, 414, 574, 612, 679
CviQI GTAC 5 cut(s) 366, 490, 551, 582, 586
DdeI CTNAG 2 cut(s) 251, 560
DpnI GATC 2 cut(s) 123, 165
DpnII GATC 2 cut(s) 121, 163
EaeI YGGCCR 1 cut(s) 677
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
Eco105I TACGTA 1 cut(s) 585
Eco130I CCWWGG 1 cut(s) 310
Eco31I GGTCTC 1 cut(s) 290
Eco57I CTGAAG 1 cut(s) 422
EcoRI GAATTC 2 cut(s) 341, 419
EcoT14I CCWWGG 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 3 cut(s) 283, 453, 632
FatI CATG 3 cut(s) 279, 449, 628
FblI GTMKAC 3 cut(s) 26, 66, 194
Fnu4HI GCNGC 2 cut(s) 44, 99
FokI GGATG 1 cut(s) 284
Fsp4HI GCNGC 2 cut(s) 44, 99
FspBI CTAG 2 cut(s) 311, 644
GluI GCNGC 2 cut(s) 44, 99
HaeIII GGCC 1 cut(s) 679
HapII CCGG 2 cut(s) 377, 579
Hin1II CATG 3 cut(s) 283, 453, 632
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 2 cut(s) 203, 268
HpaII CCGG 2 cut(s) 377, 579
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 4 cut(s) 27, 67, 195, 242
Hpy188I TCNGA 3 cut(s) 202, 208, 347
Hpy188III TCNNGA 1 cut(s) 178
Hpy8I GTNNAC 4 cut(s) 27, 67, 195, 242
HpyAV CCTTC 3 cut(s) 312, 428, 490
HpyCH4III ACNGT 3 cut(s) 59, 356, 494
HpyCH4IV ACGT 1 cut(s) 584
HpyCH4V TGCA 3 cut(s) 216, 242, 542
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 234, 243
HpyF3I CTNAG 2 cut(s) 251, 560
HpySE526I ACGT 1 cut(s) 584
Hsp92II CATG 3 cut(s) 283, 453, 632
KpnI GGTACC 1 cut(s) 369
Kzo9I GATC 2 cut(s) 121, 163
LguI GCTCTTC 1 cut(s) 138
LpnPI CCDG 9 cut(s) 175, 205, 276, 382, 390, 492, 584, 592, 602
Lsp1109I GCAGC 2 cut(s) 30, 110
MaeI CTAG 2 cut(s) 311, 644
MaeII ACGT 1 cut(s) 584
MaeIII GTNAC 2 cut(s) 229, 256
MalI GATC 2 cut(s) 123, 165
MboI GATC 2 cut(s) 121, 163
MboII GAAGA 2 cut(s) 125, 520
MfeI CAATTG 1 cut(s) 335
MhlI GDGCHC 1 cut(s) 244
MlsI TGGCCA 1 cut(s) 679
MluCI AATT 7 cut(s) 335, 341, 380, 419, 484, 500, 598
MluNI TGGCCA 1 cut(s) 679
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 5 cut(s) 29, 152, 177, 208, 329
Mox20I TGGCCA 1 cut(s) 679
MscI TGGCCA 1 cut(s) 679
MseI TTAA 2 cut(s) 80, 555
Msp20I TGGCCA 1 cut(s) 679
MspI CCGG 2 cut(s) 377, 579
MspR9I CCNGG 1 cut(s) 377
MunI CAATTG 1 cut(s) 335
MwoI GCNNNNNNNGC 3 cut(s) 137, 234, 243
NciI CCSGG 1 cut(s) 377
NdeII GATC 2 cut(s) 121, 163
NlaIII CATG 3 cut(s) 283, 453, 632
NlaIV GGNNCC 6 cut(s) 91, 295, 367, 439, 511, 621
NmuCI GTSAC 1 cut(s) 256
PciSI GCTCTTC 1 cut(s) 138
PfeI GAWTC 2 cut(s) 203, 268
PflMI CCANNNNNTGG 1 cut(s) 189
PkrI GCNGC 2 cut(s) 45, 100
Ppu21I YACGTR 1 cut(s) 585
PspN4I GGNNCC 6 cut(s) 91, 295, 367, 439, 511, 621
RsaI GTAC 5 cut(s) 367, 491, 552, 583, 587
RsaNI GTAC 5 cut(s) 366, 490, 551, 582, 586
SalI GTCGAC 1 cut(s) 25
SapI GCTCTTC 1 cut(s) 138
SaqAI TTAA 2 cut(s) 80, 555
SatI GCNGC 2 cut(s) 44, 99
Sau3AI GATC 2 cut(s) 121, 163
ScaI AGTACT 1 cut(s) 552
ScrFI CCNGG 1 cut(s) 377
SduI GDGCHC 1 cut(s) 244
SnaBI TACGTA 1 cut(s) 585
Sse9I AATT 7 cut(s) 335, 341, 380, 419, 484, 500, 598
SsiI CCGC 1 cut(s) 237
SspMI CTAG 2 cut(s) 311, 644
StyD4I CCNGG 1 cut(s) 375
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 3 cut(s) 59, 356, 494
TaiI ACGT 1 cut(s) 587
TaqI TCGA 1 cut(s) 26
TasI AATT 7 cut(s) 335, 341, 380, 419, 484, 500, 598
TatI WGTACW 1 cut(s) 550
TfiI GAWTC 2 cut(s) 203, 268
Tru1I TTAA 2 cut(s) 80, 555
Tru9I TTAA 2 cut(s) 80, 555
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 2 cut(s) 43, 98
Tsp45I GTSAC 1 cut(s) 256
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 1 cut(s) 295
Van91I CCANNNNNTGG 1 cut(s) 189
VneI GTGCAC 1 cut(s) 240
XapI RAATTY 2 cut(s) 341, 419
XmaJI CCTAGG 1 cut(s) 310
XmiI GTMKAC 3 cut(s) 26, 66, 194
XspI CTAG 2 cut(s) 311, 644
ZrmI AGTACT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.