MD10G1277500.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000229
Physical Location & Seq
Forward (+)
36825985 .. 36826671
687 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1277500.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGCGAGAACTCGTTTCCTCTTGTCCTTAACATTGTTGCTGGTCCAGAACTGCTGTGTGTATATGCCTAAGTTAACAATAGGCGCAGTAGCAGCTCAAACCAACATCAGCACAGATGAATCAGCTCTTCGTGCTCTCAAAGCCCATATCACTAGTGACCCTGAAAACATCTTGACGACCAACTGGTCTACCTCCGATTCCAATATTTGCAACTGGGTTGGCGTTACTTGTGGTGTACGCAACCTTAGAGTCACAGCCTTGAATCTTTCTTACATGGGTCTCACAGGCACCATTCCTCCTCACCTGGGCAATCTCTCATTTCTTGTTCAGCTGGAATTCAGAAACAACAGTTTTGGTGGTACGTTGCCCTCGGAATTGTCTCATCTTCGCAGGTTGAAGTTGATTAGTTTCAGCTTCAACAACTTTGCAGGAATCATTCCATCATGGTTCGGGTCCTTATCTGAACTTCAAACCTTCGATTTGTATGGTAATCAATTTTCAGGTTCCATACCAAATGATATATTCAACTTATCTGCACTGCAAGTACTTGATCTAAGAAACAACCAGCTATCCGGTACGTACATACTTGCCAATTACCAAACTGGTGATCATTACTTAACTTTTAACACCATGTTATTAAAGAAACTTAGATTCTCTGTAAAGGATTGTTTTTCATTGATTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

229

Amino Acids

25.36

Weight (kDa)

8.3

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 77 2.3e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 87 - 186 1.3e-08 Leucine-rich repeat region
LRR_8 PF13855 130 - 190 1.4e-09 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000229)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13780 FvH4_1g13790 FvH4_3g37750
malus_domestica MD00G1192800.v1.1 MD02G1291700.v1.1 MD03G1180500.v1.1 MD03G1181600.v1.1 MD03G1181700.v1.1 MD03G1181900.v1.1 MD03G1182700.v1.1 MD03G1281400.v1.1 MD05G1297800.v1.1 MD05G1298300.v1.1 MD07G1034800.v1.1 MD10G1265200.v1.1 MD10G1277500.v1.1
prunus_persica Prupe.4G063500_v2.0.a1 Prupe.6G160500_v2.0.a1
pyrus_communis pycom04g04980 pycom05g27570 pycom05g27590 pycom10g25090
rosa_chinensis RchiOBHm_Chr2g0113621 RchiOBHm_Chr2g0113671 RchiOBHm_Chr2g0113771 RchiOBHm_Chr2g0113871 RchiOBHm_Chr2g0114031 RchiOBHm_Chr2g0114181 RchiOBHm_Chr2g0114201 RchiOBHm_Chr2g0114231 RchiOBHm_Chr2g0114241 RchiOBHm_Chr4g0403181 RchiOBHm_Chr4g0404251 RchiOBHm_Chr4g0404291 RchiOBHm_Chr4g0404471 RchiOBHm_Chr5g0010961 RchiOBHm_Chr5g0010971 RchiOBHm_Chr5g0010981 RchiOBHm_Chr5g0011131 RchiOBHm_Chr5g0011311 RchiOBHm_Chr5g0011321 RchiOBHm_Chr5g0011361 RchiOBHm_Chr5g0016031 RchiOBHm_Chr5g0040141 RchiOBHm_Chr5g0040411 RchiOBHm_Chr5g0040471 RchiOBHm_Chr5g0059151 RchiOBHm_Chr5g0059191 RchiOBHm_Chr6g0251861 RchiOBHm_Chr7g0213311 RchiOBHm_Chr7g0213321
rosa_laevigata RLG00000009001 RLG00000009062 RLG00000018075 RLG00000018085 RLG00000018097 RLG00000031818 RLG00000034199
rosa_multiflora Rmu_co7972198.1_g000001 Rmu_co8328773.1_g000001 Rmu_sc0000929.1_g000033 Rmu_sc0001023.1_g000056 Rmu_sc0001699.1_g000007 Rmu_sc0003151.1_g000028 Rmu_sc0003242.1_g000010 Rmu_sc0004283.1_g000012 Rmu_sc0004283.1_g000036 Rmu_sc0004283.1_g000048 Rmu_sc0004283.1_g000066 Rmu_sc0005187.1_g000011 Rmu_sc0007010.1_g000008 Rmu_sc0007109.1_g000001 Rmu_sc0008397.1_g000007 Rmu_sc0009151.1_g000001 Rmu_sc0009639.1_g000020 Rmu_sc0009975.1_g000007 Rmu_sc0009975.1_g000010 Rmu_sc0010543.1_g000006 Rmu_sc0011398.1_g000001 Rmu_sc0011941.1_g000025 Rmu_sc0013141.1_g000002 Rmu_sc0016457.1_g000002 Rmu_sc0038501.1_g000001 Rmu_ssc0000303.1_g000008
rosa_roxburghii Rroxscaffold_1G00040180 Rroxscaffold_1G00040280 Rroxscaffold_1G00040900 Rroxscaffold_1G00064830 Rroxscaffold_1G00065410 Rroxscaffold_2G00130010 Rroxscaffold_2G00130040
rosa_rugosa Rorug02G0188500 Rorug02G0188800 Rorug02G0188900 Rorug02G0189500 Rorug04G0458400 Rorug05G0184800
rosa_samantha Rh1CG253300 Rh2CG247500 Rh2CG247600 Rh2CG247900 Rh2CG248300 Rh2CG249100 Rh2CG249400 Rh2CG249800 Rh2CG249900 Rh2CG250000 Rh2CG250100 Rh2CG250200 Rh2CG262700 Rh4AG118100 Rh4AG118300 Rh4AG364100 Rh4BG106900 Rh4CG125100 Rh4DG110100 Rh4DG110500 Rh4DG110600 Rh4DG369800 Rh5CG093500 Rh5CG093600 Rh5CG093700 Rh5CG095300 Rh5CG095500 Rh5CG096100 Rh5CG096200 Rh5CG096400 Rh5CG099600 Rh5CG134600 Rh5CG421300 Rh6DG142900 Rh7AG370200
rosa_wichuraiana Rw0G001470 Rw0G014660 Rw0G017510 Rw0G017750 Rw1G005500 Rw2G018440 Rw2G018460 Rw2G018490 Rw2G019150 Rw4G009550 Rw5G007590 Rw5G007860 Rw6G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 184
Acc36I ACCTGC 1 cut(s) 381
AccB1I GGYRCC 1 cut(s) 287
AccI GTMKAC 1 cut(s) 188
AcsI RAATTY 1 cut(s) 335
AfaI GTAC 5 cut(s) 237, 361, 546, 577, 581
AfiI CCNNNNNNNGG 1 cut(s) 305
AgsI TTSAA 5 cut(s) 262, 397, 418, 470, 526
AhlI ACTAGT 1 cut(s) 152
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 5 cut(s) 95, 125, 331, 414, 568
AluI AGCT 5 cut(s) 95, 125, 331, 414, 568
Alw21I GWGCWC 1 cut(s) 136
Alw26I GTCTC 2 cut(s) 284, 384
ApeKI GCWGC 1 cut(s) 92
ApoI RAATTY 1 cut(s) 335
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 43, 453
AsuHPI GGTGA 2 cut(s) 293, 617
AvaII GGWCC 2 cut(s) 43, 453
BanI GGYRCC 1 cut(s) 287
Bbv12I GWGCWC 1 cut(s) 136
BbvI GCAGC 1 cut(s) 104
BccI CCATC 1 cut(s) 448
BciT130I CCWGG 1 cut(s) 305
BclI TGATCA 1 cut(s) 607
BcoDI GTCTC 2 cut(s) 284, 384
BcuI ACTAGT 1 cut(s) 152
BfaI CTAG 1 cut(s) 153
BfuAI ACCTGC 1 cut(s) 381
BisI GCNGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 94
BmcAI AGTACT 1 cut(s) 546
Bme1390I CCNGG 1 cut(s) 305
Bme18I GGWCC 2 cut(s) 43, 453
BmgT120I GGNCC 2 cut(s) 43, 453
BmiI GGNNCC 3 cut(s) 289, 454, 505
BmrFI CCNGG 1 cut(s) 305
BmrI ACTGGG 1 cut(s) 223
BmuI ACTGGG 1 cut(s) 223
BsaAI YACGTR 1 cut(s) 579
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 304, 369
BsaWI WCCGGW 1 cut(s) 572
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse1I ACTGG 3 cut(s) 188, 218, 607
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 2 cut(s) 304, 369
BseLI CCNNNNNNNGG 1 cut(s) 305
BseNI ACTGG 3 cut(s) 188, 218, 607
BseRI GAGGAG 1 cut(s) 288
BseXI GCAGC 1 cut(s) 104
BsgI GTGCAG 1 cut(s) 519
BshNI GGYRCC 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 136
BsiSI CCGG 1 cut(s) 573
BslI CCNNNNNNNGG 1 cut(s) 305
BsmAI GTCTC 2 cut(s) 284, 384
Bso31I GGTCTC 1 cut(s) 284
Bsp1286I GDGCHC 1 cut(s) 136
Bsp143I GATC 2 cut(s) 550, 607
BspLI GGNNCC 3 cut(s) 289, 454, 505
BspMI ACCTGC 1 cut(s) 381
BspQI GCTCTTC 1 cut(s) 132
BspT107I GGYRCC 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 284
BsrI ACTGG 3 cut(s) 188, 218, 607
BssECI CCNNGG 2 cut(s) 304, 369
BssMI GATC 2 cut(s) 550, 607
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 350
Bst6I CTCTTC 1 cut(s) 132
BstBAI YACGTR 1 cut(s) 579
BstDEI CTNAG 4 cut(s) 69, 245, 554, 647
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 2 cut(s) 553, 610
BstMAI GTCTC 2 cut(s) 284, 384
BstMBI GATC 2 cut(s) 550, 607
BstMWI GCNNNNNNNGC 3 cut(s) 92, 131, 140
BstNI CCWGG 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 303
BstSNI TACGTA 1 cut(s) 579
BstV1I GCAGC 1 cut(s) 104
BtsI GCAGTG 1 cut(s) 536
BtsIMutI CAGTG 1 cut(s) 536
BveI ACCTGC 1 cut(s) 381
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 2 cut(s) 43, 453
Csp6I GTAC 5 cut(s) 236, 360, 545, 576, 580
CviAII CATG 3 cut(s) 274, 444, 631
CviJI RGCY 7 cut(s) 95, 125, 143, 257, 331, 414, 568
CviKI_1 RGCY 7 cut(s) 95, 125, 143, 257, 331, 414, 568
CviQI GTAC 5 cut(s) 236, 360, 545, 576, 580
DdeI CTNAG 4 cut(s) 69, 245, 554, 647
DpnI GATC 2 cut(s) 552, 609
DpnII GATC 2 cut(s) 550, 607
DrdI GACNNNNNNGTC 1 cut(s) 184
DseDI GACNNNNNNGTC 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco105I TACGTA 1 cut(s) 579
Eco31I GGTCTC 1 cut(s) 284
Eco47I GGWCC 2 cut(s) 43, 453
EcoO109I RGGNCCY 1 cut(s) 453
EcoRI GAATTC 1 cut(s) 335
EcoRII CCWGG 1 cut(s) 303
FaeI CATG 3 cut(s) 277, 447, 634
FatI CATG 3 cut(s) 273, 443, 630
FbaI TGATCA 1 cut(s) 607
FblI GTMKAC 1 cut(s) 188
Fnu4HI GCNGC 1 cut(s) 93
Fsp4HI GCNGC 1 cut(s) 93
FspBI CTAG 1 cut(s) 153
GlaI GCGC 1 cut(s) 85
GluI GCNGC 1 cut(s) 93
HapII CCGG 1 cut(s) 573
HhaI GCGC 1 cut(s) 86
Hin1II CATG 3 cut(s) 277, 447, 634
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HinfI GANTC 6 cut(s) 119, 197, 249, 262, 432, 651
HpaI GTTAAC 1 cut(s) 75
HpaII CCGG 1 cut(s) 573
HphI GGTGA 2 cut(s) 293, 617
Hpy166II GTNNAC 3 cut(s) 75, 189, 236
Hpy188I TCNGA 4 cut(s) 196, 341, 373, 463
Hpy188III TCNNGA 2 cut(s) 46, 172
Hpy8I GTNNAC 3 cut(s) 75, 189, 236
HpyAV CCTTC 1 cut(s) 484
HpyCH4III ACNGT 1 cut(s) 350
HpyCH4IV ACGT 2 cut(s) 362, 578
HpyCH4V TGCA 4 cut(s) 210, 428, 536, 541
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 131, 140
HpyF3I CTNAG 4 cut(s) 69, 245, 554, 647
HpySE526I ACGT 2 cut(s) 362, 578
Hsp92II CATG 3 cut(s) 277, 447, 634
HspAI GCGC 1 cut(s) 84
Ksp22I TGATCA 1 cut(s) 607
KspAI GTTAAC 1 cut(s) 75
Kzo9I GATC 2 cut(s) 550, 607
LguI GCTCTTC 1 cut(s) 132
Lsp1109I GCAGC 1 cut(s) 104
MaeI CTAG 1 cut(s) 153
MaeII ACGT 2 cut(s) 362, 578
MaeIII GTNAC 3 cut(s) 155, 223, 250
MalI GATC 2 cut(s) 552, 609
MboI GATC 2 cut(s) 550, 607
MboII GAAGA 2 cut(s) 119, 377
MhlI GDGCHC 1 cut(s) 136
MluCI AATT 4 cut(s) 335, 374, 494, 592
MlyI GAGTC 1 cut(s) 258
MnlI CCTC 5 cut(s) 29, 202, 306, 309, 379
MseI TTAA 6 cut(s) 29, 74, 617, 624, 638, 685
MspA1I CMGCKG 1 cut(s) 331
MspI CCGG 1 cut(s) 573
MspR9I CCNGG 1 cut(s) 305
MvaI CCWGG 1 cut(s) 305
MwoI GCNNNNNNNGC 3 cut(s) 92, 131, 140
NdeII GATC 2 cut(s) 550, 607
NlaIII CATG 3 cut(s) 277, 447, 634
NlaIV GGNNCC 3 cut(s) 289, 454, 505
NmuCI GTSAC 2 cut(s) 155, 250
PciSI GCTCTTC 1 cut(s) 132
PfeI GAWTC 5 cut(s) 119, 197, 262, 432, 651
PkrI GCNGC 1 cut(s) 94
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Ppu21I YACGTR 1 cut(s) 579
PpuMI RGGWCCY 1 cut(s) 453
Psp5II RGGWCCY 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 3 cut(s) 289, 454, 505
PspPI GGNCC 2 cut(s) 43, 453
PspPPI RGGWCCY 1 cut(s) 453
PvuII CAGCTG 1 cut(s) 331
RsaI GTAC 5 cut(s) 237, 361, 546, 577, 581
RsaNI GTAC 5 cut(s) 236, 360, 545, 576, 580
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 6 cut(s) 29, 74, 617, 624, 638, 685
SatI GCNGC 1 cut(s) 93
Sau3AI GATC 2 cut(s) 550, 607
Sau96I GGNCC 2 cut(s) 43, 453
ScaI AGTACT 1 cut(s) 546
SchI GAGTC 1 cut(s) 258
ScrFI CCNGG 1 cut(s) 305
SduI GDGCHC 1 cut(s) 136
SinI GGWCC 2 cut(s) 43, 453
SnaBI TACGTA 1 cut(s) 579
SpeI ACTAGT 1 cut(s) 152
Sse9I AATT 4 cut(s) 335, 374, 494, 592
SspI AATATT 1 cut(s) 205
SspMI CTAG 1 cut(s) 153
StyD4I CCNGG 1 cut(s) 303
TaaI ACNGT 1 cut(s) 350
TaiI ACGT 2 cut(s) 365, 581
TaqI TCGA 1 cut(s) 477
TasI AATT 4 cut(s) 335, 374, 494, 592
TatI WGTACW 1 cut(s) 544
TfiI GAWTC 5 cut(s) 119, 197, 262, 432, 651
Tru1I TTAA 6 cut(s) 29, 74, 617, 624, 638, 685
Tru9I TTAA 6 cut(s) 29, 74, 617, 624, 638, 685
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 2 cut(s) 155, 250
TseI GCWGC 1 cut(s) 92
Tsp45I GTSAC 2 cut(s) 155, 250
TspDTI ATGAA 2 cut(s) 132, 663
TspRI CASTG 1 cut(s) 543
VpaK11BI GGWCC 2 cut(s) 43, 453
XapI RAATTY 1 cut(s) 335
XmiI GTMKAC 1 cut(s) 188
XspI CTAG 1 cut(s) 153
ZrmI AGTACT 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.