MD05G1298300.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000229
Physical Location & Seq
Forward (+)
43180615 .. 43182878
2264 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1298300.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGCAATTTCACATTTTTCACATCACCGACGGCCTGTACAAAATGGCGACAACTTGTTTTCTCTTGTCCATTACTTTGCTGCTGGTCCAGAACTGCTGTATGTATATGCCTAACTTCACAATAGGCGCAGTAGCAACAAAATCCAACATCAGCACAGATGAGTCGGCTCTTAGTGCTCTGAAAGCTCATGTCACTAGTGACCCTCAAAACATCTTGACGACCAACTGGTCTACCTCCAATTCCAATATTTGCAGCTGGATCGGCGTTACTTGCAGTCTACGTCACCTCAGAGTCACAGCCTTGAATCTCTCTTACATGAATCTCACAGGCACTATCCCTCCCCACTTAGGCAACCTCTCATTTCTTGTTCGACTGGAATTCGGAAACAACAGTTTCCATGGCACCTTGCCCCAGGAATTGTCCCGCTTGCGCCGATTGACAAGAGTTAGCTTCGCGAACAACATCTTTGTGGGGACCATCCCATCATGGTTCGGGTCCTTATCTAAACTTCAAAGGATGAGACTGAATGGTAATCAGTTTTCTGAAATTCCAAAGGAGATGGGATTTTTAGATCAGTTGGAGGACTTGTATATGCAGTTCAATGCCCTGAAGGGACCTCTTCCTATGGCTGTCTTCAACATATCTACTTTGACTACTCTAACTGTATACAAAAACAGCTTGATTGGAAGTATTCCAGACAACATATGTCAGCACCTTCCAAATATTCAGGTGTTGCATTTGGGTAGCAACCAGTTTTCTGGGCCACTTCCTTCCAAATTAGGGCAATGCAAAGGGCTTGGTATATTGTTAATGGGGGAGAACAATTTCACTGGAACCATACCCAAAAATATTGGCAATTTGACCCAGCTGACAGCGTTATATCTTGGCTTCAACAATTTGACAGGTACAATACCAAATGAGATTGGCAATCTTCAAAATTTAGAGACTTTGACATTTGGGGATAATAATCTCAATGGCCTCGTCCCAGCCACAATCTTCAACATATCCATGATAAGAGAAATAGGGCTTTCTGAAAGTTCTAGGATGGTACTAGTTTAA

Protein Analysis

353

Amino Acids

38.78

Weight (kDa)

8.47

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 51 - 92 5.2e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 91 - 222 2.9e-10 Leucine-rich repeat region
LRR_8 PF13855 144 - 203 1.2e-06 Leucine rich repeat
LRR_8 PF13855 289 - 344 3.5e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000229)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13780 FvH4_1g13790 FvH4_3g37750
malus_domestica MD00G1192800.v1.1 MD02G1291700.v1.1 MD03G1180500.v1.1 MD03G1181600.v1.1 MD03G1181700.v1.1 MD03G1181900.v1.1 MD03G1182700.v1.1 MD03G1281400.v1.1 MD05G1297800.v1.1 MD05G1298300.v1.1 MD07G1034800.v1.1 MD10G1265200.v1.1 MD10G1277500.v1.1
prunus_persica Prupe.4G063500_v2.0.a1 Prupe.6G160500_v2.0.a1
pyrus_communis pycom04g04980 pycom05g27570 pycom05g27590 pycom10g25090
rosa_chinensis RchiOBHm_Chr2g0113621 RchiOBHm_Chr2g0113671 RchiOBHm_Chr2g0113771 RchiOBHm_Chr2g0113871 RchiOBHm_Chr2g0114031 RchiOBHm_Chr2g0114181 RchiOBHm_Chr2g0114201 RchiOBHm_Chr2g0114231 RchiOBHm_Chr2g0114241 RchiOBHm_Chr4g0403181 RchiOBHm_Chr4g0404251 RchiOBHm_Chr4g0404291 RchiOBHm_Chr4g0404471 RchiOBHm_Chr5g0010961 RchiOBHm_Chr5g0010971 RchiOBHm_Chr5g0010981 RchiOBHm_Chr5g0011131 RchiOBHm_Chr5g0011311 RchiOBHm_Chr5g0011321 RchiOBHm_Chr5g0011361 RchiOBHm_Chr5g0016031 RchiOBHm_Chr5g0040141 RchiOBHm_Chr5g0040411 RchiOBHm_Chr5g0040471 RchiOBHm_Chr5g0059151 RchiOBHm_Chr5g0059191 RchiOBHm_Chr6g0251861 RchiOBHm_Chr7g0213311 RchiOBHm_Chr7g0213321
rosa_laevigata RLG00000009001 RLG00000009062 RLG00000018075 RLG00000018085 RLG00000018097 RLG00000031818 RLG00000034199
rosa_multiflora Rmu_co7972198.1_g000001 Rmu_co8328773.1_g000001 Rmu_sc0000929.1_g000033 Rmu_sc0001023.1_g000056 Rmu_sc0001699.1_g000007 Rmu_sc0003151.1_g000028 Rmu_sc0003242.1_g000010 Rmu_sc0004283.1_g000012 Rmu_sc0004283.1_g000036 Rmu_sc0004283.1_g000048 Rmu_sc0004283.1_g000066 Rmu_sc0005187.1_g000011 Rmu_sc0007010.1_g000008 Rmu_sc0007109.1_g000001 Rmu_sc0008397.1_g000007 Rmu_sc0009151.1_g000001 Rmu_sc0009639.1_g000020 Rmu_sc0009975.1_g000007 Rmu_sc0009975.1_g000010 Rmu_sc0010543.1_g000006 Rmu_sc0011398.1_g000001 Rmu_sc0011941.1_g000025 Rmu_sc0013141.1_g000002 Rmu_sc0016457.1_g000002 Rmu_sc0038501.1_g000001 Rmu_ssc0000303.1_g000008
rosa_roxburghii Rroxscaffold_1G00040180 Rroxscaffold_1G00040280 Rroxscaffold_1G00040900 Rroxscaffold_1G00064830 Rroxscaffold_1G00065410 Rroxscaffold_2G00130010 Rroxscaffold_2G00130040
rosa_rugosa Rorug02G0188500 Rorug02G0188800 Rorug02G0188900 Rorug02G0189500 Rorug04G0458400 Rorug05G0184800
rosa_samantha Rh1CG253300 Rh2CG247500 Rh2CG247600 Rh2CG247900 Rh2CG248300 Rh2CG249100 Rh2CG249400 Rh2CG249800 Rh2CG249900 Rh2CG250000 Rh2CG250100 Rh2CG250200 Rh2CG262700 Rh4AG118100 Rh4AG118300 Rh4AG364100 Rh4BG106900 Rh4CG125100 Rh4DG110100 Rh4DG110500 Rh4DG110600 Rh4DG369800 Rh5CG093500 Rh5CG093600 Rh5CG093700 Rh5CG095300 Rh5CG095500 Rh5CG096100 Rh5CG096200 Rh5CG096400 Rh5CG099600 Rh5CG134600 Rh5CG421300 Rh6DG142900 Rh7AG370200
rosa_wichuraiana Rw0G001470 Rw0G014660 Rw0G017510 Rw0G017750 Rw1G005500 Rw2G018440 Rw2G018460 Rw2G018490 Rw2G019150 Rw4G009550 Rw5G007590 Rw5G007860 Rw6G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 226
AccB1I GGYRCC 1 cut(s) 401
AccI GTMKAC 3 cut(s) 230, 277, 666
AccII CGCG 1 cut(s) 455
AciI CCGC 1 cut(s) 424
AclWI GGATC 1 cut(s) 266
AcsI RAATTY 3 cut(s) 377, 546, 937
AcuI CTGAAG 1 cut(s) 629
AfaI GTAC 3 cut(s) 38, 907, 1050
AfiI CCNNNNNNNGG 2 cut(s) 347, 780
AgsI TTSAA 7 cut(s) 304, 512, 601, 637, 892, 935, 1000
AhlI ACTAGT 2 cut(s) 194, 1051
AjnI CCWGG 1 cut(s) 411
AluBI AGCT 5 cut(s) 185, 255, 450, 678, 868
AluI AGCT 5 cut(s) 185, 255, 450, 678, 868
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 2 cut(s) 514, 938
AlwI GGATC 1 cut(s) 266
AoxI GGCC 3 cut(s) 31, 761, 976
ApeKI GCWGC 2 cut(s) 79, 252
ApoI RAATTY 3 cut(s) 377, 546, 937
Asp700I GAANNNNTTC 1 cut(s) 824
AspLEI GCGC 2 cut(s) 128, 432
AspS9I GGNCC 5 cut(s) 85, 474, 495, 614, 761
AsuHPI GGTGA 2 cut(s) 16, 275
AvaII GGWCC 4 cut(s) 85, 474, 495, 614
BanI GGYRCC 1 cut(s) 401
BbsI GAAGAC 1 cut(s) 625
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 2 cut(s) 66, 264
BccI CCATC 4 cut(s) 485, 490, 553, 1039
BceAI ACGGC 1 cut(s) 46
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BciT130I CCWGG 1 cut(s) 413
BcoDI GTCTC 2 cut(s) 514, 938
BcuI ACTAGT 2 cut(s) 194, 1051
BfaI CTAG 3 cut(s) 195, 1041, 1052
BisI GCNGC 2 cut(s) 80, 253
BlsI GCNGC 2 cut(s) 81, 254
Bme1390I CCNGG 1 cut(s) 413
Bme18I GGWCC 4 cut(s) 85, 474, 495, 614
BmgT120I GGNCC 5 cut(s) 85, 474, 495, 614, 761
BmiI GGNNCC 5 cut(s) 403, 475, 496, 615, 835
BmrFI CCNGG 1 cut(s) 413
BpiI GAAGAC 1 cut(s) 625
BsaBI GATNNNNATC 1 cut(s) 966
BsaJI CCNNGG 2 cut(s) 397, 411
BsaXI ACNNNNNCTCC 4 cut(s) 322, 352, 572, 602
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 780
Bse1I ACTGG 4 cut(s) 230, 378, 751, 835
Bse3DI GCAATG 1 cut(s) 791
Bse8I GATNNNNATC 1 cut(s) 966
BseBI CCWGG 1 cut(s) 413
BseDI CCNNGG 2 cut(s) 397, 411
BseGI GGATG 3 cut(s) 477, 522, 1050
BseJI GATNNNNATC 1 cut(s) 966
BseLI CCNNNNNNNGG 2 cut(s) 347, 780
BseMI GCAATG 1 cut(s) 791
BseMII CTCAG 1 cut(s) 301
BseNI ACTGG 4 cut(s) 230, 378, 751, 835
BseXI GCAGC 2 cut(s) 66, 264
BseYI CCCAGC 2 cut(s) 864, 985
Bsh1236I CGCG 1 cut(s) 455
BshFI GGCC 3 cut(s) 33, 763, 978
BshNI GGYRCC 1 cut(s) 401
BsiHKAI GWGCWC 1 cut(s) 178
BslFI GGGAC 4 cut(s) 406, 487, 627, 968
BslI CCNNNNNNNGG 2 cut(s) 347, 780
BsmAI GTCTC 2 cut(s) 514, 938
BsmFI GGGAC 4 cut(s) 406, 487, 627, 968
BsnI GGCC 3 cut(s) 33, 763, 978
Bsp1286I GDGCHC 1 cut(s) 178
Bsp1407I TGTACA 1 cut(s) 36
Bsp143I GATC 2 cut(s) 258, 571
Bsp19I CCATGG 1 cut(s) 397
Bsp68I TCGCGA 1 cut(s) 455
BspACI CCGC 1 cut(s) 424
BspANI GGCC 3 cut(s) 33, 763, 978
BspCNI CTCAG 1 cut(s) 300
BspFNI CGCG 1 cut(s) 455
BspLI GGNNCC 5 cut(s) 403, 475, 496, 615, 835
BspPI GGATC 1 cut(s) 266
BspT107I GGYRCC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 791
BsrGI TGTACA 1 cut(s) 36
BsrI ACTGG 4 cut(s) 230, 378, 751, 835
BssECI CCNNGG 2 cut(s) 397, 411
BssMI GATC 2 cut(s) 258, 571
BssNAI GTATAC 1 cut(s) 667
BssT1I CCWWGG 1 cut(s) 397
Bst1107I GTATAC 1 cut(s) 667
Bst2UI CCWGG 1 cut(s) 413
Bst4CI ACNGT 2 cut(s) 392, 664
Bst6I CTCTTC 1 cut(s) 624
BstAUI TGTACA 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 428
BstDEI CTNAG 3 cut(s) 170, 287, 346
BstDSI CCRYGG 1 cut(s) 397
BstF5I GGATG 3 cut(s) 477, 522, 1050
BstFNI CGCG 1 cut(s) 455
BstHHI GCGC 2 cut(s) 128, 432
BstKTI GATC 2 cut(s) 261, 574
BstMAI GTCTC 2 cut(s) 514, 938
BstMBI GATC 2 cut(s) 258, 571
BstMWI GCNNNNNNNGC 4 cut(s) 173, 182, 261, 270
BstNI CCWGG 1 cut(s) 413
BstSCI CCNGG 1 cut(s) 411
BstUI CGCG 1 cut(s) 455
BstV1I GCAGC 2 cut(s) 66, 264
BstV2I GAAGAC 1 cut(s) 625
BstXI CCANNNNNNTGG 1 cut(s) 758
BstZ17I GTATAC 1 cut(s) 667
BsuRI GGCC 3 cut(s) 33, 763, 978
BtgI CCRYGG 1 cut(s) 397
BtsCI GGATG 3 cut(s) 477, 522, 1050
BtsIMutI CAGTG 1 cut(s) 828
BtuMI TCGCGA 1 cut(s) 455
Cac8I GCNNGC 1 cut(s) 428
CfoI GCGC 2 cut(s) 128, 432
Cfr13I GGNCC 5 cut(s) 85, 474, 495, 614, 761
Csp6I GTAC 3 cut(s) 37, 906, 1049
CviAII CATG 5 cut(s) 188, 316, 398, 486, 1009
CviQI GTAC 3 cut(s) 37, 906, 1049
DdeI CTNAG 3 cut(s) 170, 287, 346
DpnI GATC 2 cut(s) 260, 573
DpnII GATC 2 cut(s) 258, 571
DrdI GACNNNNNNGTC 1 cut(s) 226
DseDI GACNNNNNNGTC 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 624
EarI CTCTTC 1 cut(s) 624
Eco130I CCWWGG 1 cut(s) 397
Eco47I GGWCC 4 cut(s) 85, 474, 495, 614
Eco57I CTGAAG 1 cut(s) 629
EcoO109I RGGNCCY 2 cut(s) 495, 614
EcoRI GAATTC 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 411
EcoT14I CCWWGG 1 cut(s) 397
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 5 cut(s) 191, 319, 401, 489, 1012
FaqI GGGAC 4 cut(s) 406, 487, 627, 968
FatI CATG 5 cut(s) 187, 315, 397, 485, 1008
FauI CCCGC 1 cut(s) 431
FauNDI CATATG 1 cut(s) 704
FblI GTMKAC 3 cut(s) 230, 277, 666
Fnu4HI GCNGC 2 cut(s) 80, 253
FokI GGATG 2 cut(s) 464, 529
Fsp4HI GCNGC 2 cut(s) 80, 253
FspBI CTAG 3 cut(s) 195, 1041, 1052
GlaI GCGC 2 cut(s) 127, 431
GluI GCNGC 2 cut(s) 80, 253
GsaI CCCAGC 2 cut(s) 868, 989
HaeIII GGCC 3 cut(s) 33, 763, 978
HhaI GCGC 2 cut(s) 128, 432
Hin1II CATG 5 cut(s) 191, 319, 401, 489, 1012
Hin6I GCGC 2 cut(s) 126, 430
HinP1I GCGC 2 cut(s) 126, 430
HinfI GANTC 4 cut(s) 161, 291, 304, 319
HphI GGTGA 2 cut(s) 16, 275
Hpy166II GTNNAC 3 cut(s) 231, 278, 667
Hpy188I TCNGA 5 cut(s) 180, 290, 383, 544, 1033
Hpy188III TCNNGA 4 cut(s) 88, 214, 454, 695
Hpy8I GTNNAC 3 cut(s) 231, 278, 667
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 3 cut(s) 604, 725, 780
HpyCH4III ACNGT 2 cut(s) 392, 664
HpyCH4IV ACGT 1 cut(s) 280
HpyCH4V TGCA 6 cut(s) 4, 252, 273, 595, 736, 789
HpyF10VI GCNNNNNNNGC 4 cut(s) 173, 182, 261, 270
HpyF3I CTNAG 3 cut(s) 170, 287, 346
HpySE526I ACGT 1 cut(s) 280
Hsp92II CATG 5 cut(s) 191, 319, 401, 489, 1012
HspAI GCGC 2 cut(s) 126, 430
Kzo9I GATC 2 cut(s) 258, 571
Lsp1109I GCAGC 2 cut(s) 66, 264
MaeI CTAG 3 cut(s) 195, 1041, 1052
MaeII ACGT 1 cut(s) 280
MaeIII GTNAC 5 cut(s) 190, 197, 265, 281, 292
MalI GATC 2 cut(s) 260, 573
MboI GATC 2 cut(s) 258, 571
MboII GAAGA 4 cut(s) 611, 625, 923, 988
MhlI GDGCHC 1 cut(s) 178
MlyI GAGTC 2 cut(s) 170, 300
MmeI TCCRAC 2 cut(s) 168, 558
MnlI CCTC 8 cut(s) 213, 244, 296, 348, 365, 574, 627, 989
MroXI GAANNNNTTC 1 cut(s) 824
MseI TTAA 2 cut(s) 809, 1057
MslI CAYNNNNRTG 2 cut(s) 467, 1007
MspA1I CMGCKG 2 cut(s) 255, 868
MspR9I CCNGG 1 cut(s) 413
MvaI CCWGG 1 cut(s) 413
MvnI CGCG 1 cut(s) 455
MwoI GCNNNNNNNGC 4 cut(s) 173, 182, 261, 270
NcoI CCATGG 1 cut(s) 397
NdeI CATATG 1 cut(s) 704
NdeII GATC 2 cut(s) 258, 571
NlaIII CATG 5 cut(s) 191, 319, 401, 489, 1012
NlaIV GGNNCC 5 cut(s) 403, 475, 496, 615, 835
NmuCI GTSAC 4 cut(s) 190, 197, 281, 292
NruI TCGCGA 1 cut(s) 455
PdmI GAANNNNTTC 1 cut(s) 824
PfeI GAWTC 2 cut(s) 304, 319
PkrI GCNGC 2 cut(s) 81, 254
PleI GAGTC 2 cut(s) 169, 299
PpsI GAGTC 2 cut(s) 169, 299
PpuMI RGGWCCY 2 cut(s) 495, 614
Psp5II RGGWCCY 2 cut(s) 495, 614
Psp6I CCWGG 1 cut(s) 411
PspFI CCCAGC 2 cut(s) 864, 985
PspGI CCWGG 1 cut(s) 411
PspN4I GGNNCC 5 cut(s) 403, 475, 496, 615, 835
PspPI GGNCC 5 cut(s) 85, 474, 495, 614, 761
PspPPI RGGWCCY 2 cut(s) 495, 614
PsrI GAACNNNNNNTAC 2 cut(s) 581, 613
PvuII CAGCTG 2 cut(s) 255, 868
RruI TCGCGA 1 cut(s) 455
RsaI GTAC 3 cut(s) 38, 907, 1050
RsaNI GTAC 3 cut(s) 37, 906, 1049
RseI CAYNNNNRTG 2 cut(s) 467, 1007
SaqAI TTAA 2 cut(s) 809, 1057
SatI GCNGC 2 cut(s) 80, 253
Sau3AI GATC 2 cut(s) 258, 571
Sau96I GGNCC 5 cut(s) 85, 474, 495, 614, 761
SchI GAGTC 2 cut(s) 170, 300
ScrFI CCNGG 1 cut(s) 413
SduI GDGCHC 1 cut(s) 178
SinI GGWCC 4 cut(s) 85, 474, 495, 614
SmiMI CAYNNNNRTG 2 cut(s) 467, 1007
SpeI ACTAGT 2 cut(s) 194, 1051
SsiI CCGC 1 cut(s) 424
SspI AATATT 3 cut(s) 247, 724, 850
SspMI CTAG 3 cut(s) 195, 1041, 1052
StyD4I CCNGG 1 cut(s) 411
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 2 cut(s) 392, 664
TaiI ACGT 1 cut(s) 283
TaqI TCGA 1 cut(s) 370
TatI WGTACW 1 cut(s) 36
TfiI GAWTC 2 cut(s) 304, 319
Tru1I TTAA 2 cut(s) 809, 1057
Tru9I TTAA 2 cut(s) 809, 1057
TscAI CASTG 1 cut(s) 835
TseFI GTSAC 4 cut(s) 190, 197, 281, 292
TseI GCWGC 2 cut(s) 79, 252
Tsp45I GTSAC 4 cut(s) 190, 197, 281, 292
TspDTI ATGAA 1 cut(s) 332
TspRI CASTG 1 cut(s) 835
VpaK11BI GGWCC 4 cut(s) 85, 474, 495, 614
XapI RAATTY 3 cut(s) 377, 546, 937
XmiI GTMKAC 3 cut(s) 230, 277, 666
XmnI GAANNNNTTC 1 cut(s) 824
XspI CTAG 3 cut(s) 195, 1041, 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.