FvH4_1g04260

eukaryotic translation initiation factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000095
Physical Location & Seq
Reverse (-)
2263224 .. 2266392
3169 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04260.t1

Sequence Viewer

Length: 1803 bp
ATGACTCTCAACTTTCAAGATGTTGACTTCTCTGTAGGTGATAATTTTCACTGCTACATAAACCCAGTACAAGAACTAAGAGATCGGAAAAGCCGTAAATATGTCAGTTGGATGGATCGTGTGTTGGAACAGCATCAAGGCCAGTACATTGAACGATTCAGCAGGGCTTACTTCTTTCTACATAGCGGGTTTAAAAATTCCATTGATAAGTGGATTGAATTTGCCATGCAAAAGAGAGTTCAAGTACTTGAGCTGCAGTTTCTTATACCAAAATATGATTATGCCAGAGATAACTATGCATTTCCCTATACCTTATTAGGTGAATCTTTTAAGCATATGTACCCTTACCTTCCAACTCTACAATCATGTCACTACAACATTGGTTTCAAATTCCTTAAGGTTGTTCGCTTCCAGCATATCGGCATGACTGATGAAGTTCTCGACTACTTCTTGTCTAATTGTCCAGTTCTTGAGAGATTAATTGTGATAGATGCAAGAGGTTTGGTTTGTTTAAGAGTTGTTGGTGCGTCAATCTCATTGAAGTATTTGACAGTAAATGGTTGTCCTAACCTCAAAAGCATTGAGATTTGCAACGCAAATATAGTTTCATTTGTCTATAGGGGAGCTTGGATGGACCTGTTTATTAGTAACGTGCCCCTTCTGATCGAGGTCTACAACCAAAATTATGACTTCCCCATATTAGAAAATCTGAAGCAATTGGAATTAAATGTTGAGACAGATTACCACTCGGCTCTTTATCATCTGTCTTCTTTCATGAAGGTTTGTCCTCACATCCACAGACTTGTACTGAAGTTGGACTTCCGAAGAACAGACGTCGAAACAACAGTAACCAAAGCTACCAAATTCCCTCATCATAGCCTCAAGGTAGTAGAAATTATGGGTTATCGTGGCTATGAATGTGCTGTTAAACATGTCGTGTACTTGATAGAGAGTGTTGTTGCACTAGAGAAGATTATTATTGATCCTGTTCGGCGTTGGATTTGGCCCCCCGGAATGGACAGGGAACCTGATATGGTGAAAGAGGAAGTGAAGGCAAGAAAGCATGCTAAGGAACACCTTCAAGAAAGAGTTCCATCTACTATAGAATTTCAATCATGGCATATTGAGGAGGAGGTGGCACCGGCAGACATTGCGATTTTTGATCCTAGTAAAAAGAAGAAGAAGAAGAAGGTTGTAATTGAGGATCCAGCTGATGATACTATGGACAAAGAAGGAGAAGGAATTATACTAGAGGCATCCAATCCTTATTTGGATTATGAATATGAGGAGCTTCTTGAAGCAGTGTACAACATTCTTAGGGAGAATAATCCTGAGCTAGCCGGAGAGAAGCGTCGGACAGTGACGAGTCCTCCGCAAGTTCTGAGCGAGGGCACAAAGAAAACCGTCTACGCGAATTTCAAGGAGCATTGCAAGACGATCCATAGGCAACCAGAGCATGTCCAGAATTTCTTTTTGGCCGAACTGGGGACAATTGGATCTCTTGATGGACAACAGAGGCTAGTTGTCAAGGGAAGGTTCACGTCGAAGACTCTTGAAAGAATACTGATGCGATATGTGAATGATTATGTGATTTGTCTTGGGTGCAAAAGCATCGACACTGTACTGTCGAAGGAGAGTCGCCTGAGTTTCATCAGATGCGAGAAGTGCGGTTCTGGACGATCAGTTGCTCCGATCAGACAAGGTTTTGTTGCTCGTGTAGGCCTTAGGCATGATCCTTCATATTCTGGTTTTGATTATATATTTGCATGCAGGAGTAATAATCTCTATCTCGTTGTAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

601

Amino Acids

69.79

Weight (kDa)

7.84

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 41 - 216 2.2e-18 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 68 - 195 1.8e-07 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1 PF23622 236 - 371 4.4e-08 At1g61320/AtMIF1, LRR domain
eIF-5_eIF-2B PF01873 449 - 558 2.1e-35 Domain found in IF2B/IF5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000095)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04260 FvH4_1g04270 FvH4_1g04271 FvH4_1g04280 FvH4_1g15681 FvH4_1g15870 FvH4_1g15871 FvH4_1g15880 FvH4_1g15890 FvH4_1g15890 FvH4_1g15890 FvH4_2g27801 FvH4_2g38801 FvH4_3g15922 FvH4_6g01850 FvH4_6g01850 FvH4_6g01850 FvH4_6g01850 FvH4_6g01861 FvH4_6g01970 FvH4_6g01970 FvH4_6g04740 FvH4_6g05140 FvH4_6g05150 FvH4_6g05160 FvH4_6g05620 FvH4_6g43901 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150
malus_domestica MD02G1016200.v1.1 MD02G1024100.v1.1 MD02G1024500.v1.1 MD02G1269100.v1.1 MD05G1360100.v1.1 MD08G1218100.v1.1 MD10G1330300.v1.1 MD11G1200500.v1.1 MD12G1064700.v1.1 MD14G1064800.v1.1 MD15G1437600.v1.1
prunus_persica Prupe.1G269300_v2.0.a1 Prupe.1G269300_v2.0.a1 Prupe.1G404400_v2.0.a1 Prupe.1G404400_v2.0.a1 Prupe.1G404600_v2.0.a1 Prupe.3G194200_v2.0.a1 Prupe.4G126500_v2.0.a1 Prupe.4G126500_v2.0.a1 Prupe.4G126500_v2.0.a1 Prupe.5G083600_v2.0.a1 Prupe.5G181600_v2.0.a1 Prupe.6G354300_v2.0.a1 Prupe.6G354300_v2.0.a1 Prupe.6G354500_v2.0.a1 Prupe.6G354600_v2.0.a1 Prupe.7G069200_v2.0.a1 Prupe.7G069300_v2.0.a1 Prupe.7G069300_v2.0.a1 Prupe.7G069300_v2.0.a1 Prupe.7G247200_v2.0.a1 Prupe.8G038200_v2.0.a1 Prupe.8G159200_v2.0.a1 Prupe.8G168400_v2.0.a1 Prupe.8G213700_v2.0.a1 Prupe.I001500_v2.0.a1
pyrus_communis pycom01g10450 pycom02g01440 pycom02g02050 pycom02g02060 pycom05g32510 pycom10g27970 pycom12g05660 pycom14g05250 pycom15g14470
rosa_chinensis RchiOBHm_Chr1g0330451 RchiOBHm_Chr1g0330541 RchiOBHm_Chr1g0360451 RchiOBHm_Chr2g0105201 RchiOBHm_Chr2g0105211 RchiOBHm_Chr2g0105241 RchiOBHm_Chr2g0105251 RchiOBHm_Chr2g0105261 RchiOBHm_Chr2g0160681 RchiOBHm_Chr3g0448841 RchiOBHm_Chr3g0448851 RchiOBHm_Chr3g0449531 RchiOBHm_Chr3g0449541 RchiOBHm_Chr3g0449551 RchiOBHm_Chr3g0449571 RchiOBHm_Chr3g0449581 RchiOBHm_Chr3g0449591 RchiOBHm_Chr3g0449601 RchiOBHm_Chr3g0449821 RchiOBHm_Chr3g0471621 RchiOBHm_Chr3g0480841 RchiOBHm_Chr5g0026831 RchiOBHm_Chr5g0026841 RchiOBHm_Chr6g0298681 RchiOBHm_Chr6g0298691 RchiOBHm_Chr6g0302471 RchiOBHm_Chr7g0184471 RchiOBHm_Chr7g0184481 RchiOBHm_Chr7g0197551
rosa_laevigata RLG00000003925 RLG00000004972 RLG00000016059 RLG00000017409 RLG00000023448 RLG00000023621 RLG00000025792 RLG00000025793 RLG00000025816 RLG00000025871 RLG00000027807 RLG00000029809 RLG00000032963
rosa_multiflora Rmu_co8092536.1_g000001 Rmu_co8349415.1_g000001 Rmu_co8375699.1_g000001 Rmu_co8381287.1_g000001 Rmu_co8442591.1_g000001 Rmu_co8442591.1_g000002 Rmu_co8446009.1_g000001 Rmu_co8468183.1_g000001 Rmu_sc0000430.1_g000001 Rmu_sc0000430.1_g000004 Rmu_sc0000430.1_g000012 Rmu_sc0000610.1_g000007 Rmu_sc0001122.1_g000011 Rmu_sc0001170.1_g000015 Rmu_sc0001407.1_g000011 Rmu_sc0001557.1_g000012 Rmu_sc0001557.1_g000013 Rmu_sc0002200.1_g000011 Rmu_sc0004831.1_g000010 Rmu_sc0004838.1_g000013 Rmu_sc0006734.1_g000010 Rmu_sc0007290.1_g000003 Rmu_sc0011021.1_g000011 Rmu_sc0012217.1_g000006 Rmu_sc0012984.1_g000017 Rmu_sc0013983.1_g000003 Rmu_sc0018347.1_g000001 Rmu_sc0021523.1_g000001 Rmu_sc0026421.1_g000001 Rmu_sc0026421.1_g000002 Rmu_sc0027913.1_g000002 Rmu_sc0037242.1_g000001 Rmu_ssc0000031.1_g000002 Rmu_ssc0000031.1_g000003 Rmu_ssc0000172.1_g000019 Rmu_ssc0000337.1_g000013 Rmu_ssc0000368.1_g000069
rosa_roxburghii Rroxscaffold_1G00013770 Rroxscaffold_1G00017620 Rroxscaffold_1G00052670 Rroxscaffold_1G00052680 Rroxscaffold_1G00052690 Rroxscaffold_2G00089490 Rroxscaffold_2G00089510 Rroxscaffold_2G00138340 Rroxscaffold_2G00138350 Rroxscaffold_2G00138380 Rroxscaffold_2G00151560 Rroxscaffold_2G00151570 Rroxscaffold_3G00220490 Rroxscaffold_3G00258350 Rroxscaffold_4G00296100 Rroxscaffold_6G00400970 Rroxscaffold_6G00403030 Rroxscaffold_6G00427050 Rroxscaffold_6G00427490 Rroxscaffold_6G00427500 Rroxscaffold_6G00427640 Rroxscaffold_6G00427660 Rroxscaffold_7G00165730 Rroxscaffold_7G00165740
rosa_rugosa Rorug01G0194900 Rorug01G0277200 Rorug01G0277300 Rorug01G0277400 Rorug01G0277500 Rorug01G0277600 Rorug01G0277700 Rorug01G0277800 Rorug02G0003600 Rorug02G0126800 Rorug02G0126900 Rorug02G0484800 Rorug02G0484900 Rorug02G0623600 Rorug03G0186800 Rorug06G0314400 Rorug07G0041200 Rorug07G0041300 Rorug07G0041300 Rorug07G0041400
rosa_samantha Rh1AG107100 Rh1AG174800 Rh1AG212900 Rh1AG291000 Rh1BG085200 Rh1DG112700 Rh2AG048800 Rh2AG177600 Rh2AG549900 Rh2BG047600 Rh2BG185700 Rh2BG185800 Rh2BG186000 Rh2BG186100 Rh2BG564400 Rh2CG049700 Rh2CG182100 Rh2CG182300 Rh3BG014900 Rh3BG020400 Rh3BG020500 Rh3BG020600 Rh3BG020700 Rh3BG020800 Rh3BG020900 Rh3BG022500 Rh3BG195200 Rh3BG271900 Rh3CG019100 Rh3CG019200 Rh3CG019300 Rh3CG019400 Rh3CG021200 Rh3CG267700 Rh3DG020400 Rh3DG020500 Rh3DG020600 Rh3DG020700 Rh3DG022200 Rh3DG264000 Rh5BG185300 Rh5BG185500 Rh5BG186000 Rh5BG429000 Rh6AG427600 Rh6CG439700 Rh6DG426700 Rh7BG067600 Rh7CG067900 Rh7CG068000 Rh7CG174300 Rh7DG167300
rosa_wichuraiana Rw0G006170 Rw1G008880 Rw1G014570 Rw1G025770 Rw2G013940 Rw2G013960 Rw2G045560 Rw3G001610 Rw3G001620 Rw3G001630 Rw3G001650 Rw3G001790 Rw3G021340 Rw4G018910 Rw5G017150 Rw5G017160 Rw7G014440 Rw7G040330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 837
AccB1I GGYRCC 1 cut(s) 1138
AccI GTMKAC 2 cut(s) 672, 1407
AccII CGCG 1 cut(s) 1412
AciI CCGC 3 cut(s) 186, 1373, 1668
AclWI GGATC 8 cut(s) 123, 977, 1157, 1199, 1212, 1432, 1504, 1727
AcoI YGGCCR 1 cut(s) 1476
AcsI RAATTY 7 cut(s) 196, 218, 389, 863, 1106, 1414, 1465
AcuI CTGAAG 2 cut(s) 731, 830
AcyI GRCGYC 1 cut(s) 834
AfaI GTAC 8 cut(s) 69, 146, 246, 341, 807, 941, 1307, 1623
AfiI CCNNNNNNNGG 2 cut(s) 1015, 1485
AflII CTTAAG 1 cut(s) 395
AflIII ACRYGT 1 cut(s) 931
AjiI CACGTC 1 cut(s) 1542
AjuI GAANNNNNNNTTGG 2 cut(s) 1457, 1489
AluBI AGCT 7 cut(s) 253, 626, 857, 1211, 1291, 1336, 1799
AluI AGCT 7 cut(s) 253, 626, 857, 1211, 1291, 1336, 1799
Alw26I GTCTC 1 cut(s) 728
AlwI GGATC 8 cut(s) 123, 977, 1157, 1199, 1212, 1432, 1504, 1727
AoxI GGCC 4 cut(s) 139, 1004, 1476, 1720
ApeKI GCWGC 1 cut(s) 253
ApoI RAATTY 7 cut(s) 196, 218, 389, 863, 1106, 1414, 1465
AseI ATTAAT 1 cut(s) 479
Asp700I GAANNNNTTC 2 cut(s) 1077, 1089
AspS9I GGNCC 2 cut(s) 634, 1005
AsuC2I CCSGG 1 cut(s) 1011
AsuHPI GGTGA 3 cut(s) 50, 332, 1048
AsuNHI GCTAGC 1 cut(s) 1336
AvaII GGWCC 1 cut(s) 634
AxyI CCTNAGG 1 cut(s) 1724
BaeGI GKGCMC 2 cut(s) 657, 1394
BamHI GGATCC 1 cut(s) 1204
BanI GGYRCC 1 cut(s) 1138
BarI GAAGNNNNNNTAC 2 cut(s) 1230, 1262
BauI CACGAG 1 cut(s) 1713
BbsI GAAGAC 2 cut(s) 759, 1553
BbvI GCAGC 1 cut(s) 240
BccI CCATC 4 cut(s) 106, 625, 1102, 1499
BceAI ACGGC 1 cut(s) 78
BcgI CGANNNNNNTGC 2 cut(s) 1594, 1628
BcnI CCSGG 1 cut(s) 1011
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 5 cut(s) 965, 1167, 1250, 1337, 1520
BfmI CTRYAG 4 cut(s) 33, 254, 616, 1101
BfrI CTTAAG 1 cut(s) 395
BisI GCNGC 1 cut(s) 254
BlsI GCNGC 1 cut(s) 255
BmcAI AGTACT 1 cut(s) 246
Bme1390I CCNGG 1 cut(s) 1011
Bme18I GGWCC 1 cut(s) 634
BmgBI CACGTC 1 cut(s) 1542
BmgT120I GGNCC 2 cut(s) 634, 1005
BmiI GGNNCC 4 cut(s) 1007, 1026, 1140, 1206
BmrFI CCNGG 1 cut(s) 1011
BmrI ACTGGG 2 cut(s) 59, 1493
BmsI GCATC 6 cut(s) 142, 481, 1265, 1557, 1620, 1646
BmtI GCTAGC 1 cut(s) 1340
BmuI ACTGGG 2 cut(s) 59, 1493
BpiI GAAGAC 2 cut(s) 759, 1553
Bpu10I CCTNAGC 2 cut(s) 1068, 1332
BpuEI CTTGAG 3 cut(s) 269, 491, 866
BpuMI CCSGG 1 cut(s) 1011
BsaHI GRCGYC 1 cut(s) 834
BsaJI CCNNGG 1 cut(s) 1009
BsaXI ACNNNNNCTCC 2 cut(s) 1354, 1384
Bsc4I CCNNNNNNNGG 2 cut(s) 1015, 1485
Bse118I RCCGGY 1 cut(s) 1141
Bse1I ACTGG 4 cut(s) 65, 142, 464, 1488
Bse21I CCTNAGG 1 cut(s) 1724
Bse3DI GCAATG 2 cut(s) 1149, 1426
BseDI CCNNGG 1 cut(s) 1009
BseGI GGATG 4 cut(s) 117, 636, 792, 1256
BseLI CCNNNNNNNGG 2 cut(s) 1015, 1485
BseMI GCAATG 2 cut(s) 1149, 1426
BseMII CTCAG 3 cut(s) 1323, 1373, 1634
BseNI ACTGG 4 cut(s) 65, 142, 464, 1488
BseRI GAGGAG 3 cut(s) 1142, 1145, 1301
BseSI GKGCMC 2 cut(s) 657, 1394
BseXI GCAGC 1 cut(s) 240
Bsh1236I CGCG 1 cut(s) 1412
BshFI GGCC 4 cut(s) 141, 1006, 1478, 1722
BshNI GGYRCC 1 cut(s) 1138
BsiSI CCGG 3 cut(s) 1011, 1142, 1341
BslFI GGGAC 1 cut(s) 1501
BslI CCNNNNNNNGG 2 cut(s) 1015, 1485
BsmAI GTCTC 1 cut(s) 728
BsmFI GGGAC 1 cut(s) 1501
BsnI GGCC 4 cut(s) 141, 1006, 1478, 1722
Bsp1286I GDGCHC 2 cut(s) 657, 1394
Bsp1407I TGTACA 1 cut(s) 1305
BspACI CCGC 3 cut(s) 186, 1373, 1668
BspANI GGCC 4 cut(s) 141, 1006, 1478, 1722
BspCNI CTCAG 3 cut(s) 1324, 1374, 1635
BspFNI CGCG 1 cut(s) 1412
BspHI TCATGA 1 cut(s) 774
BspLI GGNNCC 4 cut(s) 1007, 1026, 1140, 1206
BspMAI CTGCAG 1 cut(s) 258
BspOI GCTAGC 1 cut(s) 1340
BspPI GGATC 8 cut(s) 123, 977, 1157, 1199, 1212, 1432, 1504, 1727
BspT107I GGYRCC 1 cut(s) 1138
BspTI CTTAAG 1 cut(s) 395
BsrDI GCAATG 2 cut(s) 1149, 1426
BsrFI RCCGGY 1 cut(s) 1141
BsrGI TGTACA 1 cut(s) 1305
BsrI ACTGG 4 cut(s) 65, 142, 464, 1488
BssAI RCCGGY 1 cut(s) 1141
BssECI CCNNGG 1 cut(s) 1009
BssNI GRCGYC 1 cut(s) 834
BssSI CACGAG 1 cut(s) 1713
Bst2BI CACGAG 1 cut(s) 1713
Bst4CI ACNGT 6 cut(s) 553, 847, 1360, 1405, 1621, 1626
BstACI GRCGYC 1 cut(s) 834
BstAFI CTTAAG 1 cut(s) 395
BstAPI GCANNNNNTGC 1 cut(s) 1151
BstAUI TGTACA 1 cut(s) 1305
BstC8I GCNNGC 3 cut(s) 1065, 1338, 1768
BstDEI CTNAG 8 cut(s) 77, 1068, 1316, 1332, 1382, 1643, 1724, 1800
BstF5I GGATG 4 cut(s) 117, 636, 792, 1256
BstFNI CGCG 1 cut(s) 1412
BstMAI GTCTC 1 cut(s) 728
BstMWI GCNNNNNNNGC 3 cut(s) 1151, 1453, 1665
BstNSI RCATGY 4 cut(s) 935, 1067, 1460, 1770
BstSCI CCNGG 1 cut(s) 1009
BstSFI CTRYAG 4 cut(s) 33, 254, 616, 1101
BstSLI GKGCMC 2 cut(s) 657, 1394
BstUI CGCG 1 cut(s) 1412
BstV1I GCAGC 1 cut(s) 240
BstV2I GAAGAC 2 cut(s) 759, 1553
BstX2I RGATCY 2 cut(s) 1204, 1496
BstYI RGATCY 2 cut(s) 1204, 1496
Bsu36I CCTNAGG 1 cut(s) 1724
BsuRI GGCC 4 cut(s) 141, 1006, 1478, 1722
BtrI CACGTC 1 cut(s) 1542
BtsCI GGATG 4 cut(s) 117, 636, 792, 1256
BtsI GCAGTG 2 cut(s) 49, 1308
BtsIMutI CAGTG 4 cut(s) 49, 1308, 1365, 1617
Cac8I GCNNGC 3 cut(s) 1065, 1338, 1768
CciI TCATGA 1 cut(s) 774
Cfr10I RCCGGY 1 cut(s) 1141
Cfr13I GGNCC 2 cut(s) 634, 1005
CseI GACGC 2 cut(s) 516, 1340
Csp6I GTAC 8 cut(s) 68, 145, 245, 340, 806, 940, 1306, 1622
CviQI GTAC 8 cut(s) 68, 145, 245, 340, 806, 940, 1306, 1622
DdeI CTNAG 8 cut(s) 77, 1068, 1316, 1332, 1382, 1643, 1724, 1800
DraI TTTAAA 1 cut(s) 193
EaeI YGGCCR 1 cut(s) 1476
Eco147I AGGCCT 1 cut(s) 1722
Eco47I GGWCC 1 cut(s) 634
Eco57I CTGAAG 2 cut(s) 731, 830
Eco81I CCTNAGG 1 cut(s) 1724
EcoT22I ATGCAT 1 cut(s) 301
FalI AAGNNNNNCTT 2 cut(s) 803, 835
FaqI GGGAC 1 cut(s) 1501
FauI CCCGC 1 cut(s) 179
FauNDI CATATG 1 cut(s) 336
FblI GTMKAC 2 cut(s) 672, 1407
Fnu4HI GCNGC 1 cut(s) 254
FokI GGATG 4 cut(s) 124, 643, 779, 1243
Fsp4HI GCNGC 1 cut(s) 254
FspBI CTAG 5 cut(s) 965, 1167, 1250, 1337, 1520
GluI GCNGC 1 cut(s) 254
HaeIII GGCC 4 cut(s) 141, 1006, 1478, 1722
HapII CCGG 3 cut(s) 1011, 1142, 1341
HgaI GACGC 2 cut(s) 516, 1340
Hin1I GRCGYC 1 cut(s) 834
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HinfI GANTC 6 cut(s) 4, 156, 323, 1366, 1549, 1636
HpaII CCGG 3 cut(s) 1011, 1142, 1341
HphI GGTGA 3 cut(s) 50, 332, 1048
Hpy166II GTNNAC 6 cut(s) 25, 673, 940, 1306, 1408, 1539
Hpy188I TCNGA 9 cut(s) 87, 663, 711, 824, 1356, 1383, 1655, 1692, 1697
Hpy8I GTNNAC 6 cut(s) 25, 673, 940, 1306, 1408, 1539
Hpy99I CGWCG 3 cut(s) 839, 1356, 1546
HpyCH4III ACNGT 6 cut(s) 553, 847, 1360, 1405, 1621, 1626
HpyCH4IV ACGT 3 cut(s) 651, 834, 1541
HpyF10VI GCNNNNNNNGC 3 cut(s) 1151, 1453, 1665
HpyF3I CTNAG 8 cut(s) 77, 1068, 1316, 1332, 1382, 1643, 1724, 1800
HpySE526I ACGT 3 cut(s) 651, 834, 1541
Hsp92I GRCGYC 1 cut(s) 834
LmnI GCTCC 4 cut(s) 623, 1288, 1423, 1693
Lsp1109I GCAGC 1 cut(s) 240
LweI GCATC 6 cut(s) 142, 481, 1265, 1557, 1620, 1646
MaeI CTAG 5 cut(s) 965, 1167, 1250, 1337, 1520
MaeII ACGT 3 cut(s) 651, 834, 1541
MaeIII GTNAC 4 cut(s) 368, 647, 847, 1360
MboII GAAGA 8 cut(s) 759, 837, 982, 1189, 1192, 1195, 1198, 1558
MfeI CAATTG 2 cut(s) 716, 1491
MflI RGATCY 2 cut(s) 1204, 1496
MhlI GDGCHC 2 cut(s) 657, 1394
MlyI GAGTC 3 cut(s) 1375, 1543, 1645
MmeI TCCRAC 6 cut(s) 89, 105, 377, 795, 977, 1334
Mph1103I ATGCAT 1 cut(s) 301
MroXI GAANNNNTTC 2 cut(s) 1077, 1089
MseI TTAA 7 cut(s) 192, 330, 396, 479, 512, 725, 927
MspA1I CMGCKG 1 cut(s) 1211
MspCI CTTAAG 1 cut(s) 395
MspI CCGG 3 cut(s) 1011, 1142, 1341
MspR9I CCNGG 1 cut(s) 1011
MunI CAATTG 2 cut(s) 716, 1491
MvnI CGCG 1 cut(s) 1412
MwoI GCNNNNNNNGC 3 cut(s) 1151, 1453, 1665
NciI CCSGG 1 cut(s) 1011
NdeI CATATG 1 cut(s) 336
NheI GCTAGC 1 cut(s) 1336
NlaIV GGNNCC 4 cut(s) 1007, 1026, 1140, 1206
NmeAIII GCCGAG 1 cut(s) 728
NmuCI GTSAC 2 cut(s) 368, 1360
NsiI ATGCAT 1 cut(s) 301
NspI RCATGY 4 cut(s) 935, 1067, 1460, 1770
PaeI GCATGC 2 cut(s) 1067, 1770
PagI TCATGA 1 cut(s) 774
PceI AGGCCT 1 cut(s) 1722
PciI ACATGT 1 cut(s) 931
PcsI WCGNNNNNNNCGW 1 cut(s) 91
PdmI GAANNNNTTC 2 cut(s) 1077, 1089
PfeI GAWTC 2 cut(s) 156, 323
PkrI GCNGC 1 cut(s) 255
PleI GAGTC 3 cut(s) 1374, 1543, 1644
PpsI GAGTC 3 cut(s) 1374, 1543, 1644
PscI ACATGT 1 cut(s) 931
PshBI ATTAAT 1 cut(s) 479
PspN4I GGNNCC 4 cut(s) 1007, 1026, 1140, 1206
PspPI GGNCC 2 cut(s) 634, 1005
PstI CTGCAG 1 cut(s) 258
PsuI RGATCY 2 cut(s) 1204, 1496
PvuII CAGCTG 1 cut(s) 1211
RsaI GTAC 8 cut(s) 69, 146, 246, 341, 807, 941, 1307, 1623
RsaNI GTAC 8 cut(s) 68, 145, 245, 340, 806, 940, 1306, 1622
SaqAI TTAA 7 cut(s) 192, 330, 396, 479, 512, 725, 927
SatI GCNGC 1 cut(s) 254
Sau96I GGNCC 2 cut(s) 634, 1005
ScaI AGTACT 1 cut(s) 246
SchI GAGTC 3 cut(s) 1375, 1543, 1645
ScrFI CCNGG 1 cut(s) 1011
SduI GDGCHC 2 cut(s) 657, 1394
SfaNI GCATC 6 cut(s) 142, 481, 1265, 1557, 1620, 1646
SfcI CTRYAG 4 cut(s) 33, 254, 616, 1101
SinI GGWCC 1 cut(s) 634
SmlI CTYRAG 4 cut(s) 248, 395, 470, 881
SmoI CTYRAG 4 cut(s) 248, 395, 470, 881
SphI GCATGC 2 cut(s) 1067, 1770
SseBI AGGCCT 1 cut(s) 1722
SsiI CCGC 3 cut(s) 186, 1373, 1668
SspMI CTAG 5 cut(s) 965, 1167, 1250, 1337, 1520
StuI AGGCCT 1 cut(s) 1722
StyD4I CCNGG 1 cut(s) 1009
TaaI ACNGT 6 cut(s) 553, 847, 1360, 1405, 1621, 1626
TaiI ACGT 3 cut(s) 654, 837, 1544
TaqI TCGA 6 cut(s) 441, 666, 837, 1544, 1614, 1628
TatI WGTACW 7 cut(s) 67, 144, 244, 805, 939, 1305, 1621
TfiI GAWTC 2 cut(s) 156, 323
Tru1I TTAA 7 cut(s) 192, 330, 396, 479, 512, 725, 927
Tru9I TTAA 7 cut(s) 192, 330, 396, 479, 512, 725, 927
TscAI CASTG 4 cut(s) 56, 1308, 1365, 1624
TseFI GTSAC 2 cut(s) 368, 1360
TseI GCWGC 1 cut(s) 253
Tsp45I GTSAC 2 cut(s) 368, 1360
TspDTI ATGAA 8 cut(s) 447, 597, 763, 791, 930, 1293, 1639, 1728
TspRI CASTG 4 cut(s) 56, 1308, 1365, 1624
Vha464I CTTAAG 1 cut(s) 395
VpaK11BI GGWCC 1 cut(s) 634
VspI ATTAAT 1 cut(s) 479
XapI RAATTY 7 cut(s) 196, 218, 389, 863, 1106, 1414, 1465
XceI RCATGY 4 cut(s) 935, 1067, 1460, 1770
XcmI CCANNNNNNNNNTGG 1 cut(s) 1267
XmiI GTMKAC 2 cut(s) 672, 1407
XmnI GAANNNNTTC 2 cut(s) 1077, 1089
XspI CTAG 5 cut(s) 965, 1167, 1250, 1337, 1520
ZraI GACGTC 1 cut(s) 835
ZrmI AGTACT 1 cut(s) 246
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.