FvH4_1g04270

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000095
Physical Location & Seq
Forward (+)
2269453 .. 2271302
1850 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04270.t1

Sequence Viewer

Length: 1464 bp
ATGTTTAATACAGTGAAATATCTAGTGAATAATCTTAGAACATCAGCGAAGGACGATAGCAATTTGGGAAAGAGAAGGGAAAGTGAGGATAAGCATAACTACTTAGTTGACAGAATCAGTTTGTTGCCCTACGAAGTTCTTGTAAGTATTGCGTCTCTTTTGTCACTAAAGGAAGCAGCAGCTACTAGTGTTCTTTCTAGGCGATGGAAGAATGTGTGGAAGTATACAACGACTCTTAACTTTCAAGATCTTGACCTTTTTGTTGGTGACAATTTTTACTGCTTGAAACTCCTAGAAGAAAATCTAAGAGATGAGAAAAGCCGTAAATATGTCGATTGGGTGGATCGTGTGTTGGTACAGCATGAAGGCCACACTATTGAGCGATTCAAGGCTTTCTTCTTTCTGAACAACATTTTTAAGAATTCCATTGATAAGTGGATTGAGTTTGCAATGCAGAAGAGAGTTCAAGTACTGGAGCTGCAGTTTTTTATAAAGCAATATGATTTTACCATAGATAACTATGAATTTCCCCAAAACTTAATAGGTCTCGGTGGAGAAGGTGAATATTCGAACCACATTCCGACACAACACTTATGCCGATACAACAATGTTGGTTTCAAATTCCTCAAAGTTCTTCGTTTGCAGCAGATTTGTTTGACTGATGAAGTTCTTGAGTACTTCTTGTCTAACTGTCCAGTTCTTGAGAGATTAACTGTGACAGTTGCAAGAGGTTTGGTTTGTGTAAGAGTTGTTGGTCCGTCAATCTCATTGAAGTATCTGGCAATACTTGATTGTCCTAACCTCAAAAGCATTGAGATTTGCAACCCAAATATTGCTTCATTTGTCTATATGGGAGCTTGTATAGACCTGCTTATTAACAACGTGCCCTTGCTGATTGAGGTTCATATATCTGAGAGAGCTCTTCATTATGTTGATTTTACAACTGTTGCGTTCAACAGACTTTCATTTTGTCTTTCTCATTTGGAGATTCTGAAGCTGAATATTTCGGGAGTGGTCTACAATCCGAATTATGACTTCCCCGTATTAGAGAATTTGAAGCAACTGGAATTAAATGTTGAGACAAATTACCAGTGGGCTCTTTATCATCTGTATTCTTTCATGAAGGCCTGTCCTCACATCCACAGACTTATACTGAAGTTGGAATTCCAAACATATGTTGAAACAATAGTAAGCAAAGCTCCCAAATTCTCTCATCACAGCCTCAAGGTAGTAGAAATTATGGGTTATCGTGGGTATCAATGTGCTGTTGAACATGTCGTGTACTTGATAGAGAATGTTGTTGCACTAGAGAAAATTATAATTGATCCTGTTCGGCGTTGGATTTGGCCCCCTGGAATGGACAGGGGAACTGAAATGGTGAAAGAGGAAGTGAAGGGAAGAAAGCATGCTAAGGAACACCTGAAAGAAAGAGTTCCTTCTACATTAGAATTTGAATGCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

488

Amino Acids

56.92

Weight (kDa)

7.23

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 39 - 74 4.5e-06 F-box domain
LRR_At1g61320_AtMIF1 PF23622 117 - 485 1.5e-37 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 144 - 383 1.4e-09 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000095)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04260 FvH4_1g04270 FvH4_1g04271 FvH4_1g04280 FvH4_1g15681 FvH4_1g15870 FvH4_1g15871 FvH4_1g15880 FvH4_1g15890 FvH4_1g15890 FvH4_1g15890 FvH4_2g27801 FvH4_2g38801 FvH4_3g15922 FvH4_6g01850 FvH4_6g01850 FvH4_6g01850 FvH4_6g01850 FvH4_6g01861 FvH4_6g01970 FvH4_6g01970 FvH4_6g04740 FvH4_6g05140 FvH4_6g05150 FvH4_6g05160 FvH4_6g05620 FvH4_6g43901 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150
malus_domestica MD02G1016200.v1.1 MD02G1024100.v1.1 MD02G1024500.v1.1 MD02G1269100.v1.1 MD05G1360100.v1.1 MD08G1218100.v1.1 MD10G1330300.v1.1 MD11G1200500.v1.1 MD12G1064700.v1.1 MD14G1064800.v1.1 MD15G1437600.v1.1
prunus_persica Prupe.1G269300_v2.0.a1 Prupe.1G269300_v2.0.a1 Prupe.1G404400_v2.0.a1 Prupe.1G404400_v2.0.a1 Prupe.1G404600_v2.0.a1 Prupe.3G194200_v2.0.a1 Prupe.4G126500_v2.0.a1 Prupe.4G126500_v2.0.a1 Prupe.4G126500_v2.0.a1 Prupe.5G083600_v2.0.a1 Prupe.5G181600_v2.0.a1 Prupe.6G354300_v2.0.a1 Prupe.6G354300_v2.0.a1 Prupe.6G354500_v2.0.a1 Prupe.6G354600_v2.0.a1 Prupe.7G069200_v2.0.a1 Prupe.7G069300_v2.0.a1 Prupe.7G069300_v2.0.a1 Prupe.7G069300_v2.0.a1 Prupe.7G247200_v2.0.a1 Prupe.8G038200_v2.0.a1 Prupe.8G159200_v2.0.a1 Prupe.8G168400_v2.0.a1 Prupe.8G213700_v2.0.a1 Prupe.I001500_v2.0.a1
pyrus_communis pycom01g10450 pycom02g01440 pycom02g02050 pycom02g02060 pycom05g32510 pycom10g27970 pycom12g05660 pycom14g05250 pycom15g14470
rosa_chinensis RchiOBHm_Chr1g0330451 RchiOBHm_Chr1g0330541 RchiOBHm_Chr1g0360451 RchiOBHm_Chr2g0105201 RchiOBHm_Chr2g0105211 RchiOBHm_Chr2g0105241 RchiOBHm_Chr2g0105251 RchiOBHm_Chr2g0105261 RchiOBHm_Chr2g0160681 RchiOBHm_Chr3g0448841 RchiOBHm_Chr3g0448851 RchiOBHm_Chr3g0449531 RchiOBHm_Chr3g0449541 RchiOBHm_Chr3g0449551 RchiOBHm_Chr3g0449571 RchiOBHm_Chr3g0449581 RchiOBHm_Chr3g0449591 RchiOBHm_Chr3g0449601 RchiOBHm_Chr3g0449821 RchiOBHm_Chr3g0471621 RchiOBHm_Chr3g0480841 RchiOBHm_Chr5g0026831 RchiOBHm_Chr5g0026841 RchiOBHm_Chr6g0298681 RchiOBHm_Chr6g0298691 RchiOBHm_Chr6g0302471 RchiOBHm_Chr7g0184471 RchiOBHm_Chr7g0184481 RchiOBHm_Chr7g0197551
rosa_laevigata RLG00000003925 RLG00000004972 RLG00000016059 RLG00000017409 RLG00000023448 RLG00000023621 RLG00000025792 RLG00000025793 RLG00000025816 RLG00000025871 RLG00000027807 RLG00000029809 RLG00000032963
rosa_multiflora Rmu_co8092536.1_g000001 Rmu_co8349415.1_g000001 Rmu_co8375699.1_g000001 Rmu_co8381287.1_g000001 Rmu_co8442591.1_g000001 Rmu_co8442591.1_g000002 Rmu_co8446009.1_g000001 Rmu_co8468183.1_g000001 Rmu_sc0000430.1_g000001 Rmu_sc0000430.1_g000004 Rmu_sc0000430.1_g000012 Rmu_sc0000610.1_g000007 Rmu_sc0001122.1_g000011 Rmu_sc0001170.1_g000015 Rmu_sc0001407.1_g000011 Rmu_sc0001557.1_g000012 Rmu_sc0001557.1_g000013 Rmu_sc0002200.1_g000011 Rmu_sc0004831.1_g000010 Rmu_sc0004838.1_g000013 Rmu_sc0006734.1_g000010 Rmu_sc0007290.1_g000003 Rmu_sc0011021.1_g000011 Rmu_sc0012217.1_g000006 Rmu_sc0012984.1_g000017 Rmu_sc0013983.1_g000003 Rmu_sc0018347.1_g000001 Rmu_sc0021523.1_g000001 Rmu_sc0026421.1_g000001 Rmu_sc0026421.1_g000002 Rmu_sc0027913.1_g000002 Rmu_sc0037242.1_g000001 Rmu_ssc0000031.1_g000002 Rmu_ssc0000031.1_g000003 Rmu_ssc0000172.1_g000019 Rmu_ssc0000337.1_g000013 Rmu_ssc0000368.1_g000069
rosa_roxburghii Rroxscaffold_1G00013770 Rroxscaffold_1G00017620 Rroxscaffold_1G00052670 Rroxscaffold_1G00052680 Rroxscaffold_1G00052690 Rroxscaffold_2G00089490 Rroxscaffold_2G00089510 Rroxscaffold_2G00138340 Rroxscaffold_2G00138350 Rroxscaffold_2G00138380 Rroxscaffold_2G00151560 Rroxscaffold_2G00151570 Rroxscaffold_3G00220490 Rroxscaffold_3G00258350 Rroxscaffold_4G00296100 Rroxscaffold_6G00400970 Rroxscaffold_6G00403030 Rroxscaffold_6G00427050 Rroxscaffold_6G00427490 Rroxscaffold_6G00427500 Rroxscaffold_6G00427640 Rroxscaffold_6G00427660 Rroxscaffold_7G00165730 Rroxscaffold_7G00165740
rosa_rugosa Rorug01G0194900 Rorug01G0277200 Rorug01G0277300 Rorug01G0277400 Rorug01G0277500 Rorug01G0277600 Rorug01G0277700 Rorug01G0277800 Rorug02G0003600 Rorug02G0126800 Rorug02G0126900 Rorug02G0484800 Rorug02G0484900 Rorug02G0623600 Rorug03G0186800 Rorug06G0314400 Rorug07G0041200 Rorug07G0041300 Rorug07G0041300 Rorug07G0041400
rosa_samantha Rh1AG107100 Rh1AG174800 Rh1AG212900 Rh1AG291000 Rh1BG085200 Rh1DG112700 Rh2AG048800 Rh2AG177600 Rh2AG549900 Rh2BG047600 Rh2BG185700 Rh2BG185800 Rh2BG186000 Rh2BG186100 Rh2BG564400 Rh2CG049700 Rh2CG182100 Rh2CG182300 Rh3BG014900 Rh3BG020400 Rh3BG020500 Rh3BG020600 Rh3BG020700 Rh3BG020800 Rh3BG020900 Rh3BG022500 Rh3BG195200 Rh3BG271900 Rh3CG019100 Rh3CG019200 Rh3CG019300 Rh3CG019400 Rh3CG021200 Rh3CG267700 Rh3DG020400 Rh3DG020500 Rh3DG020600 Rh3DG020700 Rh3DG022200 Rh3DG264000 Rh5BG185300 Rh5BG185500 Rh5BG186000 Rh5BG429000 Rh6AG427600 Rh6CG439700 Rh6DG426700 Rh7BG067600 Rh7CG067900 Rh7CG068000 Rh7CG174300 Rh7DG167300
rosa_wichuraiana Rw0G006170 Rw1G008880 Rw1G014570 Rw1G025770 Rw2G013940 Rw2G013960 Rw2G045560 Rw3G001610 Rw3G001620 Rw3G001630 Rw3G001650 Rw3G001790 Rw3G021340 Rw4G018910 Rw5G017150 Rw5G017160 Rw7G014440 Rw7G040330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 491, 1319
Acc36I ACCTGC 1 cut(s) 876
AccI GTMKAC 2 cut(s) 224, 1017
AclWI GGATC 2 cut(s) 351, 1319
AcsI RAATTY 7 cut(s) 421, 524, 620, 1051, 1163, 1205, 1448
AcuI CTGAAG 2 cut(s) 1013, 1175
AfaI GTAC 4 cut(s) 357, 471, 677, 1283
AfiI CCNNNNNNNGG 1 cut(s) 1357
AflIII ACRYGT 1 cut(s) 1273
AhlI ACTAGT 1 cut(s) 185
AjnI CCWGG 1 cut(s) 1351
AjuI GAANNNNNNNTTGG 2 cut(s) 820, 852
AluBI AGCT 6 cut(s) 182, 478, 857, 920, 997, 1199
AluI AGCT 6 cut(s) 182, 478, 857, 920, 997, 1199
Alw21I GWGCWC 1 cut(s) 922
Alw26I GTCTC 3 cut(s) 159, 551, 1073
AlwI GGATC 2 cut(s) 351, 1319
AoxI GGCC 3 cut(s) 367, 1125, 1346
ApeKI GCWGC 4 cut(s) 176, 179, 478, 643
ApoI RAATTY 7 cut(s) 421, 524, 620, 1051, 1163, 1205, 1448
ArsI GACNNNNNNTTYG 2 cut(s) 951, 983
Asp700I GAANNNNTTC 1 cut(s) 1431
AspS9I GGNCC 2 cut(s) 755, 1347
AsuHPI GGTGA 3 cut(s) 278, 572, 1390
AsuII TTCGAA 1 cut(s) 569
AvaII GGWCC 1 cut(s) 755
BaeGI GKGCMC 1 cut(s) 888
BanII GRGCYC 2 cut(s) 922, 1099
Bbv12I GWGCWC 1 cut(s) 922
BbvI GCAGC 4 cut(s) 188, 191, 465, 655
BccI CCATC 1 cut(s) 198
BceAI ACGGC 1 cut(s) 306
BciT130I CCWGG 1 cut(s) 1353
BcoDI GTCTC 3 cut(s) 159, 551, 1073
BcuI ACTAGT 1 cut(s) 185
BfaI CTAG 5 cut(s) 23, 186, 198, 293, 1307
BfmI CTRYAG 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 876
BglII AGATCT 1 cut(s) 247
BisI GCNGC 4 cut(s) 177, 180, 479, 644
BlsI GCNGC 4 cut(s) 178, 181, 480, 645
BmcAI AGTACT 2 cut(s) 471, 677
Bme1390I CCNGG 1 cut(s) 1353
Bme18I GGWCC 1 cut(s) 755
BmgT120I GGNCC 2 cut(s) 755, 1347
BmiI GGNNCC 1 cut(s) 1349
BmrFI CCNGG 1 cut(s) 1353
BpmI CTGGAG 1 cut(s) 494
Bpu10I CCTNAGC 1 cut(s) 1410
Bpu14I TTCGAA 1 cut(s) 569
BpuEI CTTGAG 3 cut(s) 692, 722, 1208
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 1 cut(s) 1351
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 1357
Bse1I ACTGG 4 cut(s) 477, 695, 1068, 1090
Bse3DI GCAATG 1 cut(s) 456
BseBI CCWGG 1 cut(s) 1353
BseDI CCNNGG 1 cut(s) 1351
BseGI GGATG 1 cut(s) 1137
BseLI CCNNNNNNNGG 1 cut(s) 1357
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 1 cut(s) 903
BseNI ACTGG 4 cut(s) 477, 695, 1068, 1090
BseSI GKGCMC 1 cut(s) 888
BseXI GCAGC 4 cut(s) 188, 191, 465, 655
BshFI GGCC 3 cut(s) 369, 1127, 1348
BsiHKAI GWGCWC 1 cut(s) 922
BslI CCNNNNNNNGG 1 cut(s) 1357
BsmAI GTCTC 3 cut(s) 159, 551, 1073
BsmBI CGTCTC 1 cut(s) 159
BsmI GAATGC 1 cut(s) 1460
BsnI GGCC 3 cut(s) 369, 1127, 1348
Bso31I GGTCTC 1 cut(s) 551
Bsp119I TTCGAA 1 cut(s) 569
Bsp1286I GDGCHC 3 cut(s) 888, 922, 1099
Bsp143I GATC 3 cut(s) 247, 343, 1324
BspANI GGCC 3 cut(s) 369, 1127, 1348
BspCNI CTCAG 1 cut(s) 904
BspHI TCATGA 1 cut(s) 1119
BspLI GGNNCC 1 cut(s) 1349
BspMAI CTGCAG 1 cut(s) 483
BspMI ACCTGC 1 cut(s) 876
BspPI GGATC 2 cut(s) 351, 1319
BspQI GCTCTTC 1 cut(s) 927
BspT104I TTCGAA 1 cut(s) 569
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 456
BsrI ACTGG 4 cut(s) 477, 695, 1068, 1090
BssECI CCNNGG 1 cut(s) 1351
BssMI GATC 3 cut(s) 247, 343, 1324
BssNAI GTATAC 1 cut(s) 225
Bst1107I GTATAC 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 1353
Bst4CI ACNGT 5 cut(s) 13, 692, 715, 721, 946
Bst6I CTCTTC 2 cut(s) 452, 927
BstBI TTCGAA 1 cut(s) 569
BstC8I GCNNGC 1 cut(s) 1407
BstDEI CTNAG 5 cut(s) 35, 103, 305, 912, 1410
BstF5I GGATG 1 cut(s) 1137
BstKTI GATC 3 cut(s) 250, 346, 1327
BstMAI GTCTC 3 cut(s) 159, 551, 1073
BstMBI GATC 3 cut(s) 247, 343, 1324
BstNI CCWGG 1 cut(s) 1353
BstNSI RCATGY 2 cut(s) 1277, 1409
BstSCI CCNGG 1 cut(s) 1351
BstSFI CTRYAG 1 cut(s) 479
BstSLI GKGCMC 1 cut(s) 888
BstV1I GCAGC 4 cut(s) 188, 191, 465, 655
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BstZ17I GTATAC 1 cut(s) 225
BsuRI GGCC 3 cut(s) 369, 1127, 1348
BtgZI GCGATG 1 cut(s) 217
BtsCI GGATG 1 cut(s) 1137
BtsIMutI CAGTG 2 cut(s) 18, 1097
BveI ACCTGC 1 cut(s) 876
Cac8I GCNNGC 1 cut(s) 1407
CciI TCATGA 1 cut(s) 1119
Cfr13I GGNCC 2 cut(s) 755, 1347
CseI GACGC 1 cut(s) 141
Csp6I GTAC 4 cut(s) 356, 470, 676, 1282
CviAII CATG 4 cut(s) 362, 1120, 1274, 1406
CviQI GTAC 4 cut(s) 356, 470, 676, 1282
DdeI CTNAG 5 cut(s) 35, 103, 305, 912, 1410
DpnI GATC 3 cut(s) 249, 345, 1326
DpnII GATC 3 cut(s) 247, 343, 1324
Eam1104I CTCTTC 2 cut(s) 452, 927
EarI CTCTTC 2 cut(s) 452, 927
Ecl136II GAGCTC 1 cut(s) 920
Eco147I AGGCCT 1 cut(s) 1127
Eco24I GRGCYC 2 cut(s) 922, 1099
Eco31I GGTCTC 1 cut(s) 551
Eco47I GGWCC 1 cut(s) 755
Eco53kI GAGCTC 1 cut(s) 920
Eco57I CTGAAG 2 cut(s) 1013, 1175
EcoICRI GAGCTC 1 cut(s) 920
EcoRI GAATTC 2 cut(s) 421, 1163
EcoRII CCWGG 1 cut(s) 1351
EcoT38I GRGCYC 2 cut(s) 922, 1099
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 4 cut(s) 365, 1123, 1277, 1409
FalI AAGNNNNNCTT 4 cut(s) 380, 412, 1420, 1452
FatI CATG 4 cut(s) 361, 1119, 1273, 1405
FauNDI CATATG 1 cut(s) 1174
FblI GTMKAC 2 cut(s) 224, 1017
Fnu4HI GCNGC 4 cut(s) 177, 180, 479, 644
FokI GGATG 1 cut(s) 1124
FriOI GRGCYC 2 cut(s) 922, 1099
Fsp4HI GCNGC 4 cut(s) 177, 180, 479, 644
FspBI CTAG 5 cut(s) 23, 186, 198, 293, 1307
GluI GCNGC 4 cut(s) 177, 180, 479, 644
GsuI CTGGAG 1 cut(s) 494
HaeIII GGCC 3 cut(s) 369, 1127, 1348
HgaI GACGC 1 cut(s) 141
Hin1II CATG 4 cut(s) 365, 1123, 1277, 1409
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 4 cut(s) 114, 232, 384, 988
HphI GGTGA 3 cut(s) 278, 572, 1390
Hpy166II GTNNAC 4 cut(s) 109, 225, 1018, 1282
Hpy188I TCNGA 5 cut(s) 405, 582, 913, 993, 1026
Hpy188III TCNNGA 6 cut(s) 245, 251, 671, 701, 1008, 1120
Hpy8I GTNNAC 4 cut(s) 109, 225, 1018, 1282
HpyAV CCTTC 7 cut(s) 43, 69, 359, 551, 1117, 1387, 1446
HpyCH4III ACNGT 5 cut(s) 13, 692, 715, 721, 946
HpyCH4IV ACGT 1 cut(s) 882
HpyCH4V TGCA 7 cut(s) 449, 454, 481, 643, 725, 822, 1304
HpyF3I CTNAG 5 cut(s) 35, 103, 305, 912, 1410
HpySE526I ACGT 1 cut(s) 882
Hsp92II CATG 4 cut(s) 365, 1123, 1277, 1409
Kzo9I GATC 3 cut(s) 247, 343, 1324
LguI GCTCTTC 1 cut(s) 927
LmnI GCTCC 3 cut(s) 475, 854, 1204
Lsp1109I GCAGC 4 cut(s) 188, 191, 465, 655
MaeI CTAG 5 cut(s) 23, 186, 198, 293, 1307
MaeII ACGT 1 cut(s) 882
MaeIII GTNAC 3 cut(s) 162, 266, 715
MalI GATC 3 cut(s) 249, 345, 1326
MboI GATC 3 cut(s) 247, 343, 1324
MboII GAAGA 7 cut(s) 220, 308, 388, 469, 626, 914, 1410
MflI RGATCY 1 cut(s) 247
MhlI GDGCHC 3 cut(s) 888, 922, 1099
MlyI GAGTC 1 cut(s) 226
MmeI TCCRAC 3 cut(s) 605, 1140, 1319
MnlI CCTC 8 cut(s) 79, 635, 722, 812, 892, 1143, 1232, 1378
MroXI GAANNNNTTC 1 cut(s) 1431
MseI TTAA 7 cut(s) 6, 237, 417, 539, 710, 876, 1070
MspR9I CCNGG 1 cut(s) 1353
Mva1269I GAATGC 1 cut(s) 1460
MvaI CCWGG 1 cut(s) 1353
NdeI CATATG 1 cut(s) 1174
NdeII GATC 3 cut(s) 247, 343, 1324
NlaIII CATG 4 cut(s) 365, 1123, 1277, 1409
NlaIV GGNNCC 1 cut(s) 1349
NmuCI GTSAC 3 cut(s) 162, 266, 715
NspI RCATGY 2 cut(s) 1277, 1409
NspV TTCGAA 1 cut(s) 569
PaeI GCATGC 1 cut(s) 1409
PagI TCATGA 1 cut(s) 1119
PceI AGGCCT 1 cut(s) 1127
PciI ACATGT 1 cut(s) 1273
PciSI GCTCTTC 1 cut(s) 927
PctI GAATGC 1 cut(s) 1460
PdmI GAANNNNTTC 1 cut(s) 1431
PfeI GAWTC 3 cut(s) 114, 384, 988
PkrI GCNGC 4 cut(s) 178, 181, 480, 645
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
PscI ACATGT 1 cut(s) 1273
PsiI TTATAA 2 cut(s) 491, 1319
Psp124BI GAGCTC 1 cut(s) 922
Psp6I CCWGG 1 cut(s) 1351
PspGI CCWGG 1 cut(s) 1351
PspN4I GGNNCC 1 cut(s) 1349
PspPI GGNCC 2 cut(s) 755, 1347
PstI CTGCAG 1 cut(s) 483
PsuI RGATCY 1 cut(s) 247
RsaI GTAC 4 cut(s) 357, 471, 677, 1283
RsaNI GTAC 4 cut(s) 356, 470, 676, 1282
SacI GAGCTC 1 cut(s) 922
SapI GCTCTTC 1 cut(s) 927
SaqAI TTAA 7 cut(s) 6, 237, 417, 539, 710, 876, 1070
SatI GCNGC 4 cut(s) 177, 180, 479, 644
Sau3AI GATC 3 cut(s) 247, 343, 1324
Sau96I GGNCC 2 cut(s) 755, 1347
ScaI AGTACT 2 cut(s) 471, 677
SchI GAGTC 1 cut(s) 226
ScrFI CCNGG 1 cut(s) 1353
SduI GDGCHC 3 cut(s) 888, 922, 1099
SfcI CTRYAG 1 cut(s) 479
SfuI TTCGAA 1 cut(s) 569
SinI GGWCC 1 cut(s) 755
SmlI CTYRAG 3 cut(s) 671, 701, 1223
SmoI CTYRAG 3 cut(s) 671, 701, 1223
SpeI ACTAGT 1 cut(s) 185
SphI GCATGC 1 cut(s) 1409
SseBI AGGCCT 1 cut(s) 1127
SspI AATATT 3 cut(s) 566, 832, 1003
SspMI CTAG 5 cut(s) 23, 186, 198, 293, 1307
SstI GAGCTC 1 cut(s) 922
StuI AGGCCT 1 cut(s) 1127
StyD4I CCNGG 1 cut(s) 1351
TaaI ACNGT 5 cut(s) 13, 692, 715, 721, 946
TaiI ACGT 1 cut(s) 885
TaqI TCGA 2 cut(s) 333, 569
TatI WGTACW 3 cut(s) 469, 675, 1281
TfiI GAWTC 3 cut(s) 114, 384, 988
Tru1I TTAA 7 cut(s) 6, 237, 417, 539, 710, 876, 1070
Tru9I TTAA 7 cut(s) 6, 237, 417, 539, 710, 876, 1070
TscAI CASTG 2 cut(s) 18, 1097
TseFI GTSAC 3 cut(s) 162, 266, 715
TseI GCWGC 4 cut(s) 176, 179, 478, 643
Tsp45I GTSAC 3 cut(s) 162, 266, 715
TspDTI ATGAA 9 cut(s) 378, 537, 678, 828, 893, 914, 954, 1108, 1136
TspGWI ACGGA 1 cut(s) 747
TspRI CASTG 2 cut(s) 18, 1097
VpaK11BI GGWCC 1 cut(s) 755
XapI RAATTY 7 cut(s) 421, 524, 620, 1051, 1163, 1205, 1448
XceI RCATGY 2 cut(s) 1277, 1409
XmiI GTMKAC 2 cut(s) 224, 1017
XmnI GAANNNNTTC 1 cut(s) 1431
XspI CTAG 5 cut(s) 23, 186, 198, 293, 1307
ZrmI AGTACT 2 cut(s) 471, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.