diphthine-ammonia ligase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 800, 951 |
| Acc36I | ACCTGC | 1 cut(s) | 738 |
| AccBSI | CCGCTC | 1 cut(s) | 872 |
| AccI | GTMKAC | 2 cut(s) | 122, 722 |
| AciI | CCGC | 4 cut(s) | 55, 62, 582, 872 |
| AclWI | GGATC | 1 cut(s) | 1193 |
| AcsI | RAATTY | 3 cut(s) | 200, 281, 1322 |
| AcuI | CTGAAG | 4 cut(s) | 872, 935, 1001, 1202 |
| AfaI | GTAC | 3 cut(s) | 492, 747, 1251 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 458, 871 |
| AflIII | ACRYGT | 1 cut(s) | 505 |
| AhlI | ACTAGT | 2 cut(s) | 931, 1006 |
| AjnI | CCWGG | 2 cut(s) | 660, 864 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1294, 1326 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1290, 1322 |
| AluBI | AGCT | 9 cut(s) | 80, 219, 294, 856, 913, 975, 1024, 1076, 1127 |
| AluI | AGCT | 9 cut(s) | 80, 219, 294, 856, 913, 975, 1024, 1076, 1127 |
| Alw21I | GWGCWC | 1 cut(s) | 1129 |
| Alw26I | GTCTC | 2 cut(s) | 675, 997 |
| AlwI | GGATC | 1 cut(s) | 1193 |
| Ama87I | CYCGRG | 2 cut(s) | 530, 560 |
| AoxI | GGCC | 2 cut(s) | 873, 1238 |
| ApeKI | GCWGC | 4 cut(s) | 856, 938, 1021, 1073 |
| ApoI | RAATTY | 3 cut(s) | 200, 281, 1322 |
| AseI | ATTAAT | 1 cut(s) | 327 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 336 |
| AsuHPI | GGTGA | 3 cut(s) | 250, 652, 1085 |
| AvaI | CYCGRG | 2 cut(s) | 530, 560 |
| AxyI | CCTNAGG | 1 cut(s) | 134 |
| BanII | GRGCYC | 3 cut(s) | 444, 464, 1129 |
| Bbv12I | GWGCWC | 1 cut(s) | 1129 |
| BbvI | GCAGC | 4 cut(s) | 843, 950, 1033, 1085 |
| BccI | CCATC | 1 cut(s) | 96 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 712, 746, 1010, 1044 |
| BciT130I | CCWGG | 2 cut(s) | 662, 866 |
| BcoDI | GTCTC | 2 cut(s) | 675, 997 |
| BcuI | ACTAGT | 2 cut(s) | 931, 1006 |
| BfaI | CTAG | 4 cut(s) | 96, 759, 932, 1007 |
| BfmI | CTRYAG | 2 cut(s) | 1317, 1334 |
| BfuAI | ACCTGC | 1 cut(s) | 738 |
| BisI | GCNGC | 6 cut(s) | 62, 857, 873, 939, 1022, 1074 |
| BlpI | GCTNAGC | 1 cut(s) | 1284 |
| BlsI | GCNGC | 6 cut(s) | 63, 858, 874, 940, 1023, 1075 |
| BmcAI | AGTACT | 2 cut(s) | 492, 1251 |
| Bme1390I | CCNGG | 2 cut(s) | 662, 866 |
| BmeT110I | CYCGRG | 2 cut(s) | 530, 560 |
| BmiI | GGNNCC | 1 cut(s) | 500 |
| BmrFI | CCNGG | 2 cut(s) | 662, 866 |
| BmsI | GCATC | 2 cut(s) | 133, 995 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 513, 545, 1207, 1239 |
| BpmI | CTGGAG | 2 cut(s) | 540, 1158 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1284 |
| BpuEI | CTTGAG | 1 cut(s) | 1082 |
| BsaBI | GATNNNNATC | 1 cut(s) | 384 |
| BsaJI | CCNNGG | 1 cut(s) | 865 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 483, 513 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 458, 871 |
| Bse1I | ACTGG | 2 cut(s) | 84, 523 |
| Bse21I | CCTNAGG | 1 cut(s) | 134 |
| Bse3DI | GCAATG | 4 cut(s) | 20, 354, 749, 893 |
| Bse8I | GATNNNNATC | 1 cut(s) | 384 |
| BseBI | CCWGG | 2 cut(s) | 662, 866 |
| BseDI | CCNNGG | 1 cut(s) | 865 |
| BseJI | GATNNNNATC | 1 cut(s) | 384 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 458, 871 |
| BseMI | GCAATG | 4 cut(s) | 20, 354, 749, 893 |
| BseNI | ACTGG | 2 cut(s) | 84, 523 |
| BseXI | GCAGC | 4 cut(s) | 843, 950, 1033, 1085 |
| BsgI | GTGCAG | 1 cut(s) | 1188 |
| BshFI | GGCC | 2 cut(s) | 875, 1240 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1129 |
| BsiHKCI | CYCGRG | 2 cut(s) | 530, 560 |
| BslFI | GGGAC | 3 cut(s) | 37, 129, 466 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 458, 871 |
| BsmAI | GTCTC | 2 cut(s) | 675, 997 |
| BsmBI | CGTCTC | 1 cut(s) | 675 |
| BsmFI | GGGAC | 3 cut(s) | 37, 129, 466 |
| BsmI | GAATGC | 2 cut(s) | 447, 1174 |
| BsnI | GGCC | 2 cut(s) | 875, 1240 |
| BsoBI | CYCGRG | 2 cut(s) | 530, 560 |
| Bsp1286I | GDGCHC | 3 cut(s) | 444, 464, 1129 |
| Bsp143I | GATC | 2 cut(s) | 1198, 1276 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1284 |
| BspACI | CCGC | 4 cut(s) | 55, 62, 582, 872 |
| BspANI | GGCC | 2 cut(s) | 875, 1240 |
| BspLI | GGNNCC | 1 cut(s) | 500 |
| BspMI | ACCTGC | 1 cut(s) | 738 |
| BspPI | GGATC | 1 cut(s) | 1193 |
| BsrBI | CCGCTC | 1 cut(s) | 872 |
| BsrDI | GCAATG | 4 cut(s) | 20, 354, 749, 893 |
| BsrI | ACTGG | 2 cut(s) | 84, 523 |
| BssECI | CCNNGG | 1 cut(s) | 865 |
| BssMI | GATC | 2 cut(s) | 1198, 1276 |
| Bst2UI | CCWGG | 2 cut(s) | 662, 866 |
| Bst4CI | ACNGT | 8 cut(s) | 120, 167, 551, 710, 769, 1017, 1249, 1318 |
| BstC8I | GCNNGC | 2 cut(s) | 59, 1045 |
| BstDEI | CTNAG | 5 cut(s) | 134, 301, 774, 841, 1284 |
| BstEII | GGTNACC | 1 cut(s) | 763 |
| BstKTI | GATC | 2 cut(s) | 1201, 1279 |
| BstMAI | GTCTC | 2 cut(s) | 675, 997 |
| BstMBI | GATC | 2 cut(s) | 1198, 1276 |
| BstNI | CCWGG | 2 cut(s) | 662, 866 |
| BstNSI | RCATGY | 2 cut(s) | 509, 1047 |
| BstPI | GGTNACC | 1 cut(s) | 763 |
| BstSCI | CCNGG | 2 cut(s) | 660, 864 |
| BstSFI | CTRYAG | 2 cut(s) | 1317, 1334 |
| BstV1I | GCAGC | 4 cut(s) | 843, 950, 1033, 1085 |
| Bsu36I | CCTNAGG | 1 cut(s) | 134 |
| BsuRI | GGCC | 2 cut(s) | 875, 1240 |
| BtgZI | GCGATG | 1 cut(s) | 115 |
| BtsIMutI | CAGTG | 4 cut(s) | 22, 91, 579, 1178 |
| BveI | ACCTGC | 1 cut(s) | 738 |
| Cac8I | GCNNGC | 2 cut(s) | 59, 1045 |
| Csp6I | GTAC | 3 cut(s) | 491, 746, 1250 |
| CviAII | CATG | 9 cut(s) | 127, 245, 506, 1044, 1048, 1153, 1212, 1226, 1280 |
| CviQI | GTAC | 3 cut(s) | 491, 746, 1250 |
| DdeI | CTNAG | 5 cut(s) | 134, 301, 774, 841, 1284 |
| DpnI | GATC | 2 cut(s) | 1200, 1278 |
| DpnII | GATC | 2 cut(s) | 1198, 1276 |
| Ecl136II | GAGCTC | 1 cut(s) | 1127 |
| Eco24I | GRGCYC | 3 cut(s) | 444, 464, 1129 |
| Eco53kI | GAGCTC | 1 cut(s) | 1127 |
| Eco57I | CTGAAG | 4 cut(s) | 872, 935, 1001, 1202 |
| Eco81I | CCTNAGG | 1 cut(s) | 134 |
| Eco88I | CYCGRG | 2 cut(s) | 530, 560 |
| Eco91I | GGTNACC | 1 cut(s) | 763 |
| EcoICRI | GAGCTC | 1 cut(s) | 1127 |
| EcoO65I | GGTNACC | 1 cut(s) | 763 |
| EcoRI | GAATTC | 1 cut(s) | 281 |
| EcoRII | CCWGG | 2 cut(s) | 660, 864 |
| EcoT22I | ATGCAT | 1 cut(s) | 1049 |
| EcoT38I | GRGCYC | 3 cut(s) | 444, 464, 1129 |
| Esp3I | CGTCTC | 1 cut(s) | 675 |
| FaeI | CATG | 9 cut(s) | 130, 248, 509, 1047, 1051, 1156, 1215, 1229, 1283 |
| FaqI | GGGAC | 3 cut(s) | 37, 129, 466 |
| FatI | CATG | 9 cut(s) | 126, 244, 505, 1043, 1047, 1152, 1211, 1225, 1279 |
| FauI | CCCGC | 1 cut(s) | 62 |
| FblI | GTMKAC | 2 cut(s) | 122, 722 |
| Fnu4HI | GCNGC | 6 cut(s) | 62, 857, 873, 939, 1022, 1074 |
| FriOI | GRGCYC | 3 cut(s) | 444, 464, 1129 |
| Fsp4HI | GCNGC | 6 cut(s) | 62, 857, 873, 939, 1022, 1074 |
| FspBI | CTAG | 4 cut(s) | 96, 759, 932, 1007 |
| GluI | GCNGC | 6 cut(s) | 62, 857, 873, 939, 1022, 1074 |
| GsuI | CTGGAG | 2 cut(s) | 540, 1158 |
| HaeIII | GGCC | 2 cut(s) | 875, 1240 |
| Hin1II | CATG | 9 cut(s) | 130, 248, 509, 1047, 1051, 1156, 1215, 1229, 1283 |
| HincII | GTYRAC | 2 cut(s) | 151, 723 |
| HindII | GTYRAC | 2 cut(s) | 151, 723 |
| HinfI | GANTC | 5 cut(s) | 241, 337, 847, 886, 1036 |
| HphI | GGTGA | 3 cut(s) | 250, 652, 1085 |
| Hpy166II | GTNNAC | 6 cut(s) | 7, 123, 151, 191, 688, 723 |
| Hpy188I | TCNGA | 3 cut(s) | 503, 680, 852 |
| Hpy188III | TCNNGA | 7 cut(s) | 286, 389, 530, 562, 979, 1031, 1137 |
| Hpy8I | GTNNAC | 6 cut(s) | 7, 123, 151, 191, 688, 723 |
| Hpy99I | CGWCG | 1 cut(s) | 622 |
| HpyAV | CCTTC | 2 cut(s) | 976, 1320 |
| HpyCH4III | ACNGT | 8 cut(s) | 120, 167, 551, 710, 769, 1017, 1249, 1318 |
| HpyCH4IV | ACGT | 1 cut(s) | 684 |
| HpyF3I | CTNAG | 5 cut(s) | 134, 301, 774, 841, 1284 |
| HpySE526I | ACGT | 1 cut(s) | 684 |
| Hsp92II | CATG | 9 cut(s) | 130, 248, 509, 1047, 1051, 1156, 1215, 1229, 1283 |
| Kzo9I | GATC | 2 cut(s) | 1198, 1276 |
| LmnI | GCTCC | 2 cut(s) | 504, 869 |
| Lsp1109I | GCAGC | 4 cut(s) | 843, 950, 1033, 1085 |
| LweI | GCATC | 2 cut(s) | 133, 995 |
| MaeI | CTAG | 4 cut(s) | 96, 759, 932, 1007 |
| MaeII | ACGT | 1 cut(s) | 684 |
| MaeIII | GTNAC | 3 cut(s) | 514, 574, 763 |
| MalI | GATC | 2 cut(s) | 1200, 1278 |
| MbiI | CCGCTC | 1 cut(s) | 872 |
| MboI | GATC | 2 cut(s) | 1198, 1276 |
| MboII | GAAGA | 2 cut(s) | 290, 1195 |
| MfeI | CAATTG | 2 cut(s) | 159, 232 |
| MhlI | GDGCHC | 3 cut(s) | 444, 464, 1129 |
| MlyI | GAGTC | 1 cut(s) | 1045 |
| MmeI | TCCRAC | 2 cut(s) | 526, 570 |
| MnlI | CCTC | 7 cut(s) | 188, 257, 494, 909, 1106, 1233, 1261 |
| Mph1103I | ATGCAT | 1 cut(s) | 1049 |
| MroXI | GAANNNNTTC | 1 cut(s) | 336 |
| MseI | TTAA | 4 cut(s) | 327, 597, 602, 1160 |
| MslI | CAYNNNNRTG | 2 cut(s) | 510, 1210 |
| MspA1I | CMGCKG | 2 cut(s) | 1024, 1076 |
| MspR9I | CCNGG | 2 cut(s) | 662, 866 |
| MunI | CAATTG | 2 cut(s) | 159, 232 |
| Mva1269I | GAATGC | 2 cut(s) | 447, 1174 |
| MvaI | CCWGG | 2 cut(s) | 662, 866 |
| NdeII | GATC | 2 cut(s) | 1198, 1276 |
| NlaIII | CATG | 9 cut(s) | 130, 248, 509, 1047, 1051, 1156, 1215, 1229, 1283 |
| NlaIV | GGNNCC | 1 cut(s) | 500 |
| NmuCI | GTSAC | 2 cut(s) | 514, 574 |
| NsiI | ATGCAT | 1 cut(s) | 1049 |
| NspI | RCATGY | 2 cut(s) | 509, 1047 |
| PaeI | GCATGC | 1 cut(s) | 1047 |
| PaeR7I | CTCGAG | 2 cut(s) | 530, 560 |
| PciI | ACATGT | 1 cut(s) | 505 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 623, 690 |
| PctI | GAATGC | 2 cut(s) | 447, 1174 |
| PdmI | GAANNNNTTC | 1 cut(s) | 336 |
| PfeI | GAWTC | 4 cut(s) | 241, 337, 847, 886 |
| PflFI | GACNNNGTC | 1 cut(s) | 11 |
| PkrI | GCNGC | 6 cut(s) | 63, 858, 874, 940, 1023, 1075 |
| PleI | GAGTC | 1 cut(s) | 1044 |
| PpsI | GAGTC | 1 cut(s) | 1044 |
| PscI | ACATGT | 1 cut(s) | 505 |
| PshBI | ATTAAT | 1 cut(s) | 327 |
| PsiI | TTATAA | 2 cut(s) | 800, 951 |
| Psp124BI | GAGCTC | 1 cut(s) | 1129 |
| Psp6I | CCWGG | 2 cut(s) | 660, 864 |
| PspEI | GGTNACC | 1 cut(s) | 763 |
| PspGI | CCWGG | 2 cut(s) | 660, 864 |
| PspN4I | GGNNCC | 1 cut(s) | 500 |
| PsyI | GACNNNGTC | 1 cut(s) | 11 |
| PvuII | CAGCTG | 2 cut(s) | 1024, 1076 |
| RsaI | GTAC | 3 cut(s) | 492, 747, 1251 |
| RsaNI | GTAC | 3 cut(s) | 491, 746, 1250 |
| RseI | CAYNNNNRTG | 2 cut(s) | 510, 1210 |
| SacI | GAGCTC | 1 cut(s) | 1129 |
| SalI | GTCGAC | 1 cut(s) | 721 |
| SaqAI | TTAA | 4 cut(s) | 327, 597, 602, 1160 |
| SatI | GCNGC | 6 cut(s) | 62, 857, 873, 939, 1022, 1074 |
| Sau3AI | GATC | 2 cut(s) | 1198, 1276 |
| ScaI | AGTACT | 2 cut(s) | 492, 1251 |
| SchI | GAGTC | 1 cut(s) | 1045 |
| ScrFI | CCNGG | 2 cut(s) | 662, 866 |
| SduI | GDGCHC | 3 cut(s) | 444, 464, 1129 |
| SfaNI | GCATC | 2 cut(s) | 133, 995 |
| SfcI | CTRYAG | 2 cut(s) | 1317, 1334 |
| Sfr274I | CTCGAG | 2 cut(s) | 530, 560 |
| SlaI | CTCGAG | 2 cut(s) | 530, 560 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 510, 1210 |
| SmlI | CTYRAG | 3 cut(s) | 530, 560, 1097 |
| SmoI | CTYRAG | 3 cut(s) | 530, 560, 1097 |
| SpeI | ACTAGT | 2 cut(s) | 931, 1006 |
| SphI | GCATGC | 1 cut(s) | 1047 |
| SsiI | CCGC | 4 cut(s) | 55, 62, 582, 872 |
| SspMI | CTAG | 4 cut(s) | 96, 759, 932, 1007 |
| SstI | GAGCTC | 1 cut(s) | 1129 |
| StyD4I | CCNGG | 2 cut(s) | 660, 864 |
| TaaI | ACNGT | 8 cut(s) | 120, 167, 551, 710, 769, 1017, 1249, 1318 |
| TaiI | ACGT | 1 cut(s) | 687 |
| TaqI | TCGA | 7 cut(s) | 531, 561, 620, 722, 1123, 1254, 1266 |
| TatI | WGTACW | 2 cut(s) | 490, 1249 |
| TauI | GCSGC | 2 cut(s) | 64, 875 |
| TfiI | GAWTC | 4 cut(s) | 241, 337, 847, 886 |
| Tru1I | TTAA | 4 cut(s) | 327, 597, 602, 1160 |
| Tru9I | TTAA | 4 cut(s) | 327, 597, 602, 1160 |
| TscAI | CASTG | 4 cut(s) | 22, 91, 579, 1185 |
| TseFI | GTSAC | 2 cut(s) | 514, 574 |
| TseI | GCWGC | 4 cut(s) | 856, 938, 1021, 1073 |
| Tsp45I | GTSAC | 2 cut(s) | 514, 574 |
| TspDTI | ATGAA | 4 cut(s) | 213, 345, 687, 950 |
| TspGWI | ACGGA | 1 cut(s) | 1222 |
| TspRI | CASTG | 4 cut(s) | 22, 91, 579, 1185 |
| Tth111I | GACNNNGTC | 1 cut(s) | 11 |
| VspI | ATTAAT | 1 cut(s) | 327 |
| XapI | RAATTY | 3 cut(s) | 200, 281, 1322 |
| XceI | RCATGY | 2 cut(s) | 509, 1047 |
| XhoI | CTCGAG | 2 cut(s) | 530, 560 |
| XmiI | GTMKAC | 2 cut(s) | 122, 722 |
| XmnI | GAANNNNTTC | 1 cut(s) | 336 |
| XspI | CTAG | 4 cut(s) | 96, 759, 932, 1007 |
| ZrmI | AGTACT | 2 cut(s) | 492, 1251 |
| Zsp2I | ATGCAT | 1 cut(s) | 1049 |