Prupe.1G404400_v2.0.a1

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000095
Physical Location & Seq
Forward (+)
35602362 .. 35605167
2806 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G404400.1

Sequence Viewer

Length: 732 bp
ATGGCCAAAGAGATCATAGCCTTGGAGCAGAGATTCAATCTGAAAACTCCACTTAAGAAAATTATCATCACCAAGACGAATAGTTTTGATAATAATGTAAGAGTTGTTGGACCAGCTCTTGCATTGAAGTATTTAGAGATACGGAATTGCCGCATTGAAAGCATTGAAATCAGTTCTGCAAACCTTGTTTCATTTACTTATGCTGGAGACTGGATACGCTTGTTTCTCTGGAATGTGCCGCGGCTTGCTGAGGTGTCCCTTGCCTGTGATAGCAGAGCTTCTACAGTGGCTGTCTTCTCCACACTGCACTTCTGTCATTCTCAGCTGGAGGTCCTCACACTGAAAACAAGCCTGATCCACAAAGAGAATTATACATTTCCTGGCTTAGAAATTCTCAAACATTTGGAAGTAAAGATTGCAACTGATGAGGATTGCTGTCTCCTTCAATTAGCTTCTTTCATTAAGGCATCTCCAGAGTTGCAGAAACTGGTGTTGGAGTTGACAGGTGCGGTGAGACCGGAACATGAGATAGGAATCAAGGAAGCTGCCAATTGCTCCCGTAATTCCCTCAGGGTAGTGGAAGTGAGAGCCTATTGTGGTCGGCCCGGTGACTTGAAGCTTATCATGTACTTAATCAAGAACGCTGTTAAACTGGAAAAAATTGTTGTGCATCCTAAAGAAGTAAGGGCAGTAGATTTTGCTAGGCGGCAACTCAAAAGACTAAGTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

244

Amino Acids

27.49

Weight (kDa)

9.24

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000095)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04260 FvH4_1g04270 FvH4_1g04271 FvH4_1g04280 FvH4_1g15681 FvH4_1g15870 FvH4_1g15871 FvH4_1g15880 FvH4_1g15890 FvH4_1g15890 FvH4_1g15890 FvH4_2g27801 FvH4_2g38801 FvH4_3g15922 FvH4_6g01850 FvH4_6g01850 FvH4_6g01850 FvH4_6g01850 FvH4_6g01861 FvH4_6g01970 FvH4_6g01970 FvH4_6g04740 FvH4_6g05140 FvH4_6g05150 FvH4_6g05160 FvH4_6g05620 FvH4_6g43901 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150
malus_domestica MD02G1016200.v1.1 MD02G1024100.v1.1 MD02G1024500.v1.1 MD02G1269100.v1.1 MD05G1360100.v1.1 MD08G1218100.v1.1 MD10G1330300.v1.1 MD11G1200500.v1.1 MD12G1064700.v1.1 MD14G1064800.v1.1 MD15G1437600.v1.1
prunus_persica Prupe.1G269300_v2.0.a1 Prupe.1G269300_v2.0.a1 Prupe.1G404400_v2.0.a1 Prupe.1G404400_v2.0.a1 Prupe.1G404600_v2.0.a1 Prupe.3G194200_v2.0.a1 Prupe.4G126500_v2.0.a1 Prupe.4G126500_v2.0.a1 Prupe.4G126500_v2.0.a1 Prupe.5G083600_v2.0.a1 Prupe.5G181600_v2.0.a1 Prupe.6G354300_v2.0.a1 Prupe.6G354300_v2.0.a1 Prupe.6G354500_v2.0.a1 Prupe.6G354600_v2.0.a1 Prupe.7G069200_v2.0.a1 Prupe.7G069300_v2.0.a1 Prupe.7G069300_v2.0.a1 Prupe.7G069300_v2.0.a1 Prupe.7G247200_v2.0.a1 Prupe.8G038200_v2.0.a1 Prupe.8G159200_v2.0.a1 Prupe.8G168400_v2.0.a1 Prupe.8G213700_v2.0.a1 Prupe.I001500_v2.0.a1
pyrus_communis pycom01g10450 pycom02g01440 pycom02g02050 pycom02g02060 pycom05g32510 pycom10g27970 pycom12g05660 pycom14g05250 pycom15g14470
rosa_chinensis RchiOBHm_Chr1g0330451 RchiOBHm_Chr1g0330541 RchiOBHm_Chr1g0360451 RchiOBHm_Chr2g0105201 RchiOBHm_Chr2g0105211 RchiOBHm_Chr2g0105241 RchiOBHm_Chr2g0105251 RchiOBHm_Chr2g0105261 RchiOBHm_Chr2g0160681 RchiOBHm_Chr3g0448841 RchiOBHm_Chr3g0448851 RchiOBHm_Chr3g0449531 RchiOBHm_Chr3g0449541 RchiOBHm_Chr3g0449551 RchiOBHm_Chr3g0449571 RchiOBHm_Chr3g0449581 RchiOBHm_Chr3g0449591 RchiOBHm_Chr3g0449601 RchiOBHm_Chr3g0449821 RchiOBHm_Chr3g0471621 RchiOBHm_Chr3g0480841 RchiOBHm_Chr5g0026831 RchiOBHm_Chr5g0026841 RchiOBHm_Chr6g0298681 RchiOBHm_Chr6g0298691 RchiOBHm_Chr6g0302471 RchiOBHm_Chr7g0184471 RchiOBHm_Chr7g0184481 RchiOBHm_Chr7g0197551
rosa_laevigata RLG00000003925 RLG00000004972 RLG00000016059 RLG00000017409 RLG00000023448 RLG00000023621 RLG00000025792 RLG00000025793 RLG00000025816 RLG00000025871 RLG00000027807 RLG00000029809 RLG00000032963
rosa_multiflora Rmu_co8092536.1_g000001 Rmu_co8349415.1_g000001 Rmu_co8375699.1_g000001 Rmu_co8381287.1_g000001 Rmu_co8442591.1_g000001 Rmu_co8442591.1_g000002 Rmu_co8446009.1_g000001 Rmu_co8468183.1_g000001 Rmu_sc0000430.1_g000001 Rmu_sc0000430.1_g000004 Rmu_sc0000430.1_g000012 Rmu_sc0000610.1_g000007 Rmu_sc0001122.1_g000011 Rmu_sc0001170.1_g000015 Rmu_sc0001407.1_g000011 Rmu_sc0001557.1_g000012 Rmu_sc0001557.1_g000013 Rmu_sc0002200.1_g000011 Rmu_sc0004831.1_g000010 Rmu_sc0004838.1_g000013 Rmu_sc0006734.1_g000010 Rmu_sc0007290.1_g000003 Rmu_sc0011021.1_g000011 Rmu_sc0012217.1_g000006 Rmu_sc0012984.1_g000017 Rmu_sc0013983.1_g000003 Rmu_sc0018347.1_g000001 Rmu_sc0021523.1_g000001 Rmu_sc0026421.1_g000001 Rmu_sc0026421.1_g000002 Rmu_sc0027913.1_g000002 Rmu_sc0037242.1_g000001 Rmu_ssc0000031.1_g000002 Rmu_ssc0000031.1_g000003 Rmu_ssc0000172.1_g000019 Rmu_ssc0000337.1_g000013 Rmu_ssc0000368.1_g000069
rosa_roxburghii Rroxscaffold_1G00013770 Rroxscaffold_1G00017620 Rroxscaffold_1G00052670 Rroxscaffold_1G00052680 Rroxscaffold_1G00052690 Rroxscaffold_2G00089490 Rroxscaffold_2G00089510 Rroxscaffold_2G00138340 Rroxscaffold_2G00138350 Rroxscaffold_2G00138380 Rroxscaffold_2G00151560 Rroxscaffold_2G00151570 Rroxscaffold_3G00220490 Rroxscaffold_3G00258350 Rroxscaffold_4G00296100 Rroxscaffold_6G00400970 Rroxscaffold_6G00403030 Rroxscaffold_6G00427050 Rroxscaffold_6G00427490 Rroxscaffold_6G00427500 Rroxscaffold_6G00427640 Rroxscaffold_6G00427660 Rroxscaffold_7G00165730 Rroxscaffold_7G00165740
rosa_rugosa Rorug01G0194900 Rorug01G0277200 Rorug01G0277300 Rorug01G0277400 Rorug01G0277500 Rorug01G0277600 Rorug01G0277700 Rorug01G0277800 Rorug02G0003600 Rorug02G0126800 Rorug02G0126900 Rorug02G0484800 Rorug02G0484900 Rorug02G0623600 Rorug03G0186800 Rorug06G0314400 Rorug07G0041200 Rorug07G0041300 Rorug07G0041300 Rorug07G0041400
rosa_samantha Rh1AG107100 Rh1AG174800 Rh1AG212900 Rh1AG291000 Rh1BG085200 Rh1DG112700 Rh2AG048800 Rh2AG177600 Rh2AG549900 Rh2BG047600 Rh2BG185700 Rh2BG185800 Rh2BG186000 Rh2BG186100 Rh2BG564400 Rh2CG049700 Rh2CG182100 Rh2CG182300 Rh3BG014900 Rh3BG020400 Rh3BG020500 Rh3BG020600 Rh3BG020700 Rh3BG020800 Rh3BG020900 Rh3BG022500 Rh3BG195200 Rh3BG271900 Rh3CG019100 Rh3CG019200 Rh3CG019300 Rh3CG019400 Rh3CG021200 Rh3CG267700 Rh3DG020400 Rh3DG020500 Rh3DG020600 Rh3DG020700 Rh3DG022200 Rh3DG264000 Rh5BG185300 Rh5BG185500 Rh5BG186000 Rh5BG429000 Rh6AG427600 Rh6CG439700 Rh6DG426700 Rh7BG067600 Rh7CG067900 Rh7CG068000 Rh7CG174300 Rh7DG167300
rosa_wichuraiana Rw0G006170 Rw1G008880 Rw1G014570 Rw1G025770 Rw2G013940 Rw2G013960 Rw2G045560 Rw3G001610 Rw3G001620 Rw3G001630 Rw3G001650 Rw3G001790 Rw3G021340 Rw4G018910 Rw5G017150 Rw5G017160 Rw7G014440 Rw7G040330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 241
AciI CCGC 5 cut(s) 151, 239, 241, 509, 706
AclWI GGATC 1 cut(s) 349
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 390
AfaI GTAC 1 cut(s) 629
AflII CTTAAG 1 cut(s) 53
AgsI TTSAA 6 cut(s) 37, 127, 158, 167, 446, 616
AjnI CCWGG 1 cut(s) 379
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
AluBI AGCT 6 cut(s) 116, 278, 325, 452, 545, 619
AluI AGCT 6 cut(s) 116, 278, 325, 452, 545, 619
Alw26I GTCTC 3 cut(s) 201, 443, 508
AlwI GGATC 1 cut(s) 349
AlwNI CAGNNNCTG 2 cut(s) 290, 487
AoxI GGCC 2 cut(s) 3, 602
ApeKI GCWGC 1 cut(s) 545
ApoI RAATTY 1 cut(s) 390
AspS9I GGNCC 3 cut(s) 110, 331, 603
AsuC2I CCSGG 1 cut(s) 606
AsuHPI GGTGA 3 cut(s) 61, 523, 620
AvaII GGWCC 2 cut(s) 110, 331
AxyI CCTNAGG 1 cut(s) 569
BaeI ACNNNNGTAYC 2 cut(s) 206, 239
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 286
BbvCI CCTCAGC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 532
BciT130I CCWGG 1 cut(s) 381
BciVI GTATCC 1 cut(s) 207
BcnI CCSGG 1 cut(s) 606
BcoDI GTCTC 3 cut(s) 201, 443, 508
BfaI CTAG 1 cut(s) 702
BfmI CTRYAG 1 cut(s) 282
BfrI CTTAAG 1 cut(s) 53
BfuI GTATCC 1 cut(s) 207
BisI GCNGC 5 cut(s) 151, 239, 242, 546, 707
BlsI GCNGC 5 cut(s) 152, 240, 243, 547, 708
Bme1390I CCNGG 2 cut(s) 381, 606
Bme18I GGWCC 2 cut(s) 110, 331
BmgT120I GGNCC 3 cut(s) 110, 331, 603
BmrFI CCNGG 2 cut(s) 381, 606
BmsI GCATC 2 cut(s) 476, 679
BpiI GAAGAC 1 cut(s) 286
BpmI CTGGAG 3 cut(s) 225, 347, 456
Bpu10I CCTNAGC 1 cut(s) 249
BpuMI CCSGG 1 cut(s) 606
BsaBI GATNNNNATC 1 cut(s) 533
BsaI GGTCTC 1 cut(s) 508
BsaJI CCNNGG 2 cut(s) 21, 239
BsaWI WCCGGW 1 cut(s) 517
Bse1I ACTGG 3 cut(s) 215, 492, 657
Bse21I CCTNAGG 1 cut(s) 569
Bse8I GATNNNNATC 1 cut(s) 533
BseBI CCWGG 1 cut(s) 381
BseDI CCNNGG 2 cut(s) 21, 239
BseGI GGATG 1 cut(s) 670
BseJI GATNNNNATC 1 cut(s) 533
BseMII CTCAG 3 cut(s) 240, 335, 583
BseNI ACTGG 3 cut(s) 215, 492, 657
BseXI GCAGC 1 cut(s) 532
BsgI GTGCAG 1 cut(s) 290
Bsh1236I CGCG 1 cut(s) 241
BshFI GGCC 2 cut(s) 5, 604
BsiSI CCGG 2 cut(s) 518, 606
BslFI GGGAC 2 cut(s) 241, 711
BsmAI GTCTC 3 cut(s) 201, 443, 508
BsmFI GGGAC 2 cut(s) 241, 711
BsnI GGCC 2 cut(s) 5, 604
Bso31I GGTCTC 1 cut(s) 508
Bsp143I GATC 2 cut(s) 12, 354
BspACI CCGC 5 cut(s) 151, 239, 241, 509, 706
BspANI GGCC 2 cut(s) 5, 604
BspCNI CTCAG 3 cut(s) 241, 334, 582
BspFNI CGCG 1 cut(s) 241
BspPI GGATC 1 cut(s) 349
BspTI CTTAAG 1 cut(s) 53
BspTNI GGTCTC 1 cut(s) 508
BsrI ACTGG 3 cut(s) 215, 492, 657
BssECI CCNNGG 2 cut(s) 21, 239
BssMI GATC 2 cut(s) 12, 354
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 286
BstAFI CTTAAG 1 cut(s) 53
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 5 cut(s) 249, 321, 385, 569, 722
BstDSI CCRYGG 1 cut(s) 239
BstF5I GGATG 1 cut(s) 670
BstFNI CGCG 1 cut(s) 241
BstKTI GATC 2 cut(s) 15, 357
BstMAI GTCTC 3 cut(s) 201, 443, 508
BstMBI GATC 2 cut(s) 12, 354
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNI CCWGG 1 cut(s) 381
BstSCI CCNGG 2 cut(s) 379, 604
BstSFI CTRYAG 1 cut(s) 282
BstUI CGCG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 532
BstV2I GAAGAC 1 cut(s) 286
Bsu36I CCTNAGG 1 cut(s) 569
BsuI GTATCC 1 cut(s) 207
BsuRI GGCC 2 cut(s) 5, 604
BtgI CCRYGG 1 cut(s) 239
BtsCI GGATG 1 cut(s) 670
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 3 cut(s) 291, 302, 338
Cac8I GCNNGC 1 cut(s) 246
CaiI CAGNNNCTG 2 cut(s) 290, 487
Cfr13I GGNCC 3 cut(s) 110, 331, 603
Cfr42I CCGCGG 1 cut(s) 242
Csp6I GTAC 1 cut(s) 628
CviAII CATG 2 cut(s) 524, 625
CviQI GTAC 1 cut(s) 628
DdeI CTNAG 5 cut(s) 249, 321, 385, 569, 722
DpnI GATC 2 cut(s) 14, 356
DpnII GATC 2 cut(s) 12, 354
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 508
Eco47I GGWCC 2 cut(s) 110, 331
Eco81I CCTNAGG 1 cut(s) 569
EcoO109I RGGNCCY 1 cut(s) 331
EcoRII CCWGG 1 cut(s) 379
EcoT14I CCWWGG 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 2 cut(s) 527, 628
FaiI YATR 5 cut(s) 17, 201, 372, 525, 626
FaqI GGGAC 2 cut(s) 241, 711
FatI CATG 2 cut(s) 523, 624
Fnu4HI GCNGC 5 cut(s) 151, 239, 242, 546, 707
FokI GGATG 1 cut(s) 657
Fsp4HI GCNGC 5 cut(s) 151, 239, 242, 546, 707
FspBI CTAG 1 cut(s) 702
GluI GCNGC 5 cut(s) 151, 239, 242, 546, 707
GsuI CTGGAG 3 cut(s) 225, 347, 456
HaeIII GGCC 2 cut(s) 5, 604
HapII CCGG 2 cut(s) 518, 606
Hin1II CATG 2 cut(s) 527, 628
HincII GTYRAC 1 cut(s) 501
HindII GTYRAC 1 cut(s) 501
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 2 cut(s) 33, 534
HpaII CCGG 2 cut(s) 518, 606
HphI GGTGA 3 cut(s) 61, 523, 620
Hpy166II GTNNAC 1 cut(s) 501
Hpy188I TCNGA 1 cut(s) 42
Hpy188III TCNNGA 3 cut(s) 229, 473, 637
Hpy8I GTNNAC 1 cut(s) 501
HpyAV CCTTC 1 cut(s) 452
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 6 cut(s) 122, 179, 307, 419, 481, 670
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
HpyF3I CTNAG 5 cut(s) 249, 321, 385, 569, 722
Hsp92II CATG 2 cut(s) 527, 628
KspI CCGCGG 1 cut(s) 242
Kzo9I GATC 2 cut(s) 12, 354
LmnI GCTCC 2 cut(s) 25, 560
Lsp1109I GCAGC 1 cut(s) 532
LweI GCATC 2 cut(s) 476, 679
MaeI CTAG 1 cut(s) 702
MaeIII GTNAC 1 cut(s) 608
MalI GATC 2 cut(s) 14, 356
MboI GATC 2 cut(s) 12, 354
MboII GAAGA 1 cut(s) 286
MfeI CAATTG 1 cut(s) 550
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 8 cut(s) 60, 145, 367, 390, 446, 550, 562, 660
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 88, 474
MnlI CCTC 5 cut(s) 244, 322, 344, 421, 578
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 54, 462, 632, 648
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 241, 325
MspCI CTTAAG 1 cut(s) 53
MspI CCGG 2 cut(s) 518, 606
MspR9I CCNGG 2 cut(s) 381, 606
MunI CAATTG 1 cut(s) 550
MvaI CCWGG 1 cut(s) 381
MvnI CGCG 1 cut(s) 241
MwoI GCNNNNNNNGC 1 cut(s) 159
NciI CCSGG 1 cut(s) 606
NdeII GATC 2 cut(s) 12, 354
NlaIII CATG 2 cut(s) 527, 628
NmuCI GTSAC 1 cut(s) 608
PfeI GAWTC 2 cut(s) 33, 534
PflFI GACNNNGTC 1 cut(s) 723
PkrI GCNGC 5 cut(s) 152, 240, 243, 547, 708
PpuMI RGGWCCY 1 cut(s) 331
Psp5II RGGWCCY 1 cut(s) 331
Psp6I CCWGG 1 cut(s) 379
PspGI CCWGG 1 cut(s) 379
PspPI GGNCC 3 cut(s) 110, 331, 603
PspPPI RGGWCCY 1 cut(s) 331
PstNI CAGNNNCTG 2 cut(s) 290, 487
PsyI GACNNNGTC 1 cut(s) 723
PvuII CAGCTG 1 cut(s) 325
RsaI GTAC 1 cut(s) 629
RsaNI GTAC 1 cut(s) 628
SacII CCGCGG 1 cut(s) 242
SaqAI TTAA 4 cut(s) 54, 462, 632, 648
SatI GCNGC 5 cut(s) 151, 239, 242, 546, 707
Sau3AI GATC 2 cut(s) 12, 354
Sau96I GGNCC 3 cut(s) 110, 331, 603
ScrFI CCNGG 2 cut(s) 381, 606
SfaNI GCATC 2 cut(s) 476, 679
SfcI CTRYAG 1 cut(s) 282
Sfr303I CCGCGG 1 cut(s) 242
SgrBI CCGCGG 1 cut(s) 242
SinI GGWCC 2 cut(s) 110, 331
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
Sse9I AATT 8 cut(s) 60, 145, 367, 390, 446, 550, 562, 660
SsiI CCGC 5 cut(s) 151, 239, 241, 509, 706
SspMI CTAG 1 cut(s) 702
StyD4I CCNGG 2 cut(s) 379, 604
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 1 cut(s) 286
TasI AATT 8 cut(s) 60, 145, 367, 390, 446, 550, 562, 660
TatI WGTACW 1 cut(s) 627
TauI GCSGC 4 cut(s) 153, 241, 244, 709
TfiI GAWTC 2 cut(s) 33, 534
Tru1I TTAA 4 cut(s) 54, 462, 632, 648
Tru9I TTAA 4 cut(s) 54, 462, 632, 648
TscAI CASTG 3 cut(s) 291, 309, 345
TseFI GTSAC 1 cut(s) 608
TseI GCWGC 1 cut(s) 545
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 2 cut(s) 180, 448
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 3 cut(s) 291, 309, 345
Tth111I GACNNNGTC 1 cut(s) 723
Vha464I CTTAAG 1 cut(s) 53
VpaK11BI GGWCC 2 cut(s) 110, 331
XapI RAATTY 1 cut(s) 390
XspI CTAG 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.