diphthine-ammonia ligase activity
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| LRR_At1g61320_AtMIF1 | PF23622 | 156 - 439 | 3.3e-35 | At1g61320/AtMIF1, LRR domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 791 |
| Acc36I | ACCTGC | 1 cut(s) | 729 |
| AccB1I | GGYRCC | 1 cut(s) | 186 |
| AccBSI | CCGCTC | 1 cut(s) | 863 |
| AccI | GTMKAC | 1 cut(s) | 122 |
| AciI | CCGC | 4 cut(s) | 55, 62, 573, 863 |
| AclWI | GGATC | 1 cut(s) | 1184 |
| AcsI | RAATTY | 4 cut(s) | 200, 299, 589, 1313 |
| AcuI | CTGAAG | 4 cut(s) | 470, 863, 926, 992 |
| AfaI | GTAC | 3 cut(s) | 549, 738, 1197 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1171 |
| AflIII | ACRYGT | 2 cut(s) | 496, 1138 |
| AgeI | ACCGGT | 1 cut(s) | 183 |
| AhlI | ACTAGT | 1 cut(s) | 922 |
| AjnI | CCWGG | 1 cut(s) | 651 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1285, 1317 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1281, 1313 |
| AluBI | AGCT | 8 cut(s) | 80, 219, 312, 490, 847, 1015, 1067, 1118 |
| AluI | AGCT | 8 cut(s) | 80, 219, 312, 490, 847, 1015, 1067, 1118 |
| Alw21I | GWGCWC | 1 cut(s) | 1120 |
| Alw26I | GTCTC | 2 cut(s) | 988, 1260 |
| AlwI | GGATC | 1 cut(s) | 1184 |
| Ama87I | CYCGRG | 1 cut(s) | 521 |
| AoxI | GGCC | 2 cut(s) | 864, 1229 |
| ApeKI | GCWGC | 4 cut(s) | 847, 929, 1012, 1064 |
| ApoI | RAATTY | 4 cut(s) | 200, 299, 589, 1313 |
| AseI | ATTAAT | 1 cut(s) | 345 |
| AsiGI | ACCGGT | 1 cut(s) | 183 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 354 |
| AspS9I | GGNCC | 1 cut(s) | 1028 |
| AsuHPI | GGTGA | 4 cut(s) | 250, 308, 643, 1076 |
| AvaI | CYCGRG | 1 cut(s) | 521 |
| AvaII | GGWCC | 1 cut(s) | 1028 |
| AxyI | CCTNAGG | 1 cut(s) | 134 |
| BaeGI | GKGCMC | 1 cut(s) | 949 |
| BanI | GGYRCC | 1 cut(s) | 186 |
| BanII | GRGCYC | 1 cut(s) | 1120 |
| BbsI | GAAGAC | 1 cut(s) | 435 |
| Bbv12I | GWGCWC | 1 cut(s) | 1120 |
| BbvI | GCAGC | 4 cut(s) | 834, 941, 1024, 1076 |
| BccI | CCATC | 1 cut(s) | 96 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 703, 737 |
| BciT130I | CCWGG | 1 cut(s) | 653 |
| BcoDI | GTCTC | 2 cut(s) | 988, 1260 |
| BcuI | ACTAGT | 1 cut(s) | 922 |
| BfaI | CTAG | 4 cut(s) | 96, 420, 923, 1263 |
| BfmI | CTRYAG | 2 cut(s) | 1308, 1325 |
| BfuAI | ACCTGC | 1 cut(s) | 729 |
| BisI | GCNGC | 6 cut(s) | 62, 848, 864, 930, 1013, 1065 |
| BlpI | GCTNAGC | 1 cut(s) | 1275 |
| BlsI | GCNGC | 6 cut(s) | 63, 849, 865, 931, 1014, 1066 |
| Bme1390I | CCNGG | 1 cut(s) | 653 |
| Bme18I | GGWCC | 1 cut(s) | 1028 |
| BmeT110I | CYCGRG | 1 cut(s) | 521 |
| BmgT120I | GGNCC | 1 cut(s) | 1028 |
| BmiI | GGNNCC | 2 cut(s) | 188, 1029 |
| BmrFI | CCNGG | 1 cut(s) | 653 |
| BmsI | GCATC | 3 cut(s) | 133, 886, 986 |
| BpiI | GAAGAC | 1 cut(s) | 435 |
| BpmI | CTGGAG | 1 cut(s) | 1193 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1275 |
| BpuEI | CTTGAG | 2 cut(s) | 572, 1073 |
| BsaBI | GATNNNNATC | 2 cut(s) | 402, 709 |
| BsaI | GGTCTC | 1 cut(s) | 1260 |
| BsaWI | WCCGGW | 2 cut(s) | 183, 544 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1171 |
| Bse118I | RCCGGY | 1 cut(s) | 183 |
| Bse1I | ACTGG | 3 cut(s) | 84, 514, 1176 |
| Bse21I | CCTNAGG | 1 cut(s) | 134 |
| Bse3DI | GCAATG | 4 cut(s) | 20, 372, 740, 884 |
| Bse8I | GATNNNNATC | 2 cut(s) | 402, 709 |
| BseBI | CCWGG | 1 cut(s) | 653 |
| BseGI | GGATG | 1 cut(s) | 877 |
| BseJI | GATNNNNATC | 2 cut(s) | 402, 709 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1171 |
| BseMI | GCAATG | 4 cut(s) | 20, 372, 740, 884 |
| BseMII | CTCAG | 1 cut(s) | 741 |
| BseNI | ACTGG | 3 cut(s) | 84, 514, 1176 |
| BseSI | GKGCMC | 1 cut(s) | 949 |
| BseXI | GCAGC | 4 cut(s) | 834, 941, 1024, 1076 |
| BshFI | GGCC | 2 cut(s) | 866, 1231 |
| BshNI | GGYRCC | 1 cut(s) | 186 |
| BshTI | ACCGGT | 1 cut(s) | 183 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1120 |
| BsiHKCI | CYCGRG | 1 cut(s) | 521 |
| BsiSI | CCGG | 2 cut(s) | 184, 545 |
| BslFI | GGGAC | 2 cut(s) | 37, 129 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1171 |
| BsmAI | GTCTC | 2 cut(s) | 988, 1260 |
| BsmFI | GGGAC | 2 cut(s) | 37, 129 |
| BsnI | GGCC | 2 cut(s) | 866, 1231 |
| Bso31I | GGTCTC | 1 cut(s) | 1260 |
| BsoBI | CYCGRG | 1 cut(s) | 521 |
| Bsp1286I | GDGCHC | 2 cut(s) | 949, 1120 |
| Bsp143I | GATC | 3 cut(s) | 666, 710, 1189 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1275 |
| BspACI | CCGC | 4 cut(s) | 55, 62, 573, 863 |
| BspANI | GGCC | 2 cut(s) | 866, 1231 |
| BspCNI | CTCAG | 1 cut(s) | 742 |
| BspLI | GGNNCC | 2 cut(s) | 188, 1029 |
| BspMI | ACCTGC | 1 cut(s) | 729 |
| BspPI | GGATC | 1 cut(s) | 1184 |
| BspT107I | GGYRCC | 1 cut(s) | 186 |
| BspTNI | GGTCTC | 1 cut(s) | 1260 |
| BsrBI | CCGCTC | 1 cut(s) | 863 |
| BsrDI | GCAATG | 4 cut(s) | 20, 372, 740, 884 |
| BsrFI | RCCGGY | 1 cut(s) | 183 |
| BsrI | ACTGG | 3 cut(s) | 84, 514, 1176 |
| BssAI | RCCGGY | 1 cut(s) | 183 |
| BssMI | GATC | 3 cut(s) | 666, 710, 1189 |
| Bst2UI | CCWGG | 1 cut(s) | 653 |
| Bst4CI | ACNGT | 5 cut(s) | 120, 377, 542, 760, 1309 |
| BstC8I | GCNNGC | 2 cut(s) | 59, 1036 |
| BstDEI | CTNAG | 4 cut(s) | 134, 750, 765, 1275 |
| BstF5I | GGATG | 1 cut(s) | 877 |
| BstKTI | GATC | 3 cut(s) | 669, 713, 1192 |
| BstMAI | GTCTC | 2 cut(s) | 988, 1260 |
| BstMBI | GATC | 3 cut(s) | 666, 710, 1189 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 775 |
| BstNI | CCWGG | 1 cut(s) | 653 |
| BstNSI | RCATGY | 2 cut(s) | 500, 1142 |
| BstSCI | CCNGG | 1 cut(s) | 651 |
| BstSFI | CTRYAG | 2 cut(s) | 1308, 1325 |
| BstSLI | GKGCMC | 1 cut(s) | 949 |
| BstV1I | GCAGC | 4 cut(s) | 834, 941, 1024, 1076 |
| BstV2I | GAAGAC | 1 cut(s) | 435 |
| Bsu36I | CCTNAGG | 1 cut(s) | 134 |
| BsuRI | GGCC | 2 cut(s) | 866, 1231 |
| BtgZI | GCGATG | 1 cut(s) | 115 |
| BtsCI | GGATG | 1 cut(s) | 877 |
| BtsI | GCAGTG | 1 cut(s) | 776 |
| BtsIMutI | CAGTG | 6 cut(s) | 22, 91, 382, 570, 776, 1169 |
| BveI | ACCTGC | 1 cut(s) | 729 |
| Cac8I | GCNNGC | 2 cut(s) | 59, 1036 |
| Cfr10I | RCCGGY | 1 cut(s) | 183 |
| Cfr13I | GGNCC | 1 cut(s) | 1028 |
| Csp6I | GTAC | 3 cut(s) | 548, 737, 1196 |
| CspAI | ACCGGT | 1 cut(s) | 183 |
| CviAII | CATG | 8 cut(s) | 245, 497, 1039, 1139, 1144, 1203, 1217, 1271 |
| CviQI | GTAC | 3 cut(s) | 548, 737, 1196 |
| DdeI | CTNAG | 4 cut(s) | 134, 750, 765, 1275 |
| DpnI | GATC | 3 cut(s) | 668, 712, 1191 |
| DpnII | GATC | 3 cut(s) | 666, 710, 1189 |
| DraI | TTTAAA | 1 cut(s) | 960 |
| Ecl136II | GAGCTC | 1 cut(s) | 1118 |
| Eco24I | GRGCYC | 1 cut(s) | 1120 |
| Eco31I | GGTCTC | 1 cut(s) | 1260 |
| Eco47I | GGWCC | 1 cut(s) | 1028 |
| Eco53kI | GAGCTC | 1 cut(s) | 1118 |
| Eco57I | CTGAAG | 4 cut(s) | 470, 863, 926, 992 |
| Eco81I | CCTNAGG | 1 cut(s) | 134 |
| Eco88I | CYCGRG | 1 cut(s) | 521 |
| EcoICRI | GAGCTC | 1 cut(s) | 1118 |
| EcoRI | GAATTC | 1 cut(s) | 299 |
| EcoRII | CCWGG | 1 cut(s) | 651 |
| EcoT38I | GRGCYC | 1 cut(s) | 1120 |
| FaeI | CATG | 8 cut(s) | 248, 500, 1042, 1142, 1147, 1206, 1220, 1274 |
| FaqI | GGGAC | 2 cut(s) | 37, 129 |
| FatI | CATG | 8 cut(s) | 244, 496, 1038, 1138, 1143, 1202, 1216, 1270 |
| FauI | CCCGC | 1 cut(s) | 62 |
| FblI | GTMKAC | 1 cut(s) | 122 |
| Fnu4HI | GCNGC | 6 cut(s) | 62, 848, 864, 930, 1013, 1065 |
| FokI | GGATG | 1 cut(s) | 864 |
| FriOI | GRGCYC | 1 cut(s) | 1120 |
| Fsp4HI | GCNGC | 6 cut(s) | 62, 848, 864, 930, 1013, 1065 |
| FspBI | CTAG | 4 cut(s) | 96, 420, 923, 1263 |
| GluI | GCNGC | 6 cut(s) | 62, 848, 864, 930, 1013, 1065 |
| GsuI | CTGGAG | 1 cut(s) | 1193 |
| HaeIII | GGCC | 2 cut(s) | 866, 1231 |
| HapII | CCGG | 2 cut(s) | 184, 545 |
| Hin1II | CATG | 8 cut(s) | 248, 500, 1042, 1142, 1147, 1206, 1220, 1274 |
| HincII | GTYRAC | 1 cut(s) | 151 |
| HindII | GTYRAC | 1 cut(s) | 151 |
| HinfI | GANTC | 4 cut(s) | 241, 355, 838, 996 |
| HpaII | CCGG | 2 cut(s) | 184, 545 |
| HphI | GGTGA | 4 cut(s) | 250, 308, 643, 1076 |
| Hpy166II | GTNNAC | 4 cut(s) | 7, 123, 151, 679 |
| Hpy188I | TCNGA | 5 cut(s) | 494, 671, 751, 843, 906 |
| Hpy188III | TCNNGA | 3 cut(s) | 304, 521, 970 |
| Hpy8I | GTNNAC | 4 cut(s) | 7, 123, 151, 679 |
| HpyAV | CCTTC | 2 cut(s) | 967, 1311 |
| HpyCH4III | ACNGT | 5 cut(s) | 120, 377, 542, 760, 1309 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 775 |
| HpyF3I | CTNAG | 4 cut(s) | 134, 750, 765, 1275 |
| Hsp92II | CATG | 8 cut(s) | 248, 500, 1042, 1142, 1147, 1206, 1220, 1274 |
| Kzo9I | GATC | 3 cut(s) | 666, 710, 1189 |
| LmnI | GCTCC | 2 cut(s) | 495, 860 |
| Lsp1109I | GCAGC | 4 cut(s) | 834, 941, 1024, 1076 |
| LweI | GCATC | 3 cut(s) | 133, 886, 986 |
| MaeI | CTAG | 4 cut(s) | 96, 420, 923, 1263 |
| MaeIII | GTNAC | 3 cut(s) | 505, 565, 754 |
| MalI | GATC | 3 cut(s) | 668, 712, 1191 |
| MbiI | CCGCTC | 1 cut(s) | 863 |
| MboI | GATC | 3 cut(s) | 666, 710, 1189 |
| MboII | GAAGA | 2 cut(s) | 440, 476 |
| MfeI | CAATTG | 3 cut(s) | 159, 232, 290 |
| MhlI | GDGCHC | 2 cut(s) | 949, 1120 |
| MlyI | GAGTC | 1 cut(s) | 990 |
| MmeI | TCCRAC | 2 cut(s) | 517, 561 |
| MnlI | CCTC | 7 cut(s) | 257, 485, 745, 850, 1097, 1224, 1253 |
| MroXI | GAANNNNTTC | 1 cut(s) | 354 |
| MseI | TTAA | 5 cut(s) | 345, 593, 648, 959, 1151 |
| MslI | CAYNNNNRTG | 1 cut(s) | 501 |
| MspA1I | CMGCKG | 2 cut(s) | 1015, 1067 |
| MspI | CCGG | 2 cut(s) | 184, 545 |
| MspR9I | CCNGG | 1 cut(s) | 653 |
| MunI | CAATTG | 3 cut(s) | 159, 232, 290 |
| MvaI | CCWGG | 1 cut(s) | 653 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 775 |
| NdeII | GATC | 3 cut(s) | 666, 710, 1189 |
| NlaIII | CATG | 8 cut(s) | 248, 500, 1042, 1142, 1147, 1206, 1220, 1274 |
| NlaIV | GGNNCC | 2 cut(s) | 188, 1029 |
| NmuCI | GTSAC | 2 cut(s) | 505, 565 |
| NspI | RCATGY | 2 cut(s) | 500, 1142 |
| PaeR7I | CTCGAG | 1 cut(s) | 521 |
| PciI | ACATGT | 2 cut(s) | 496, 1138 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 614 |
| PdmI | GAANNNNTTC | 1 cut(s) | 354 |
| PfeI | GAWTC | 3 cut(s) | 241, 355, 838 |
| PflFI | GACNNNGTC | 1 cut(s) | 11 |
| PinAI | ACCGGT | 1 cut(s) | 183 |
| PkrI | GCNGC | 6 cut(s) | 63, 849, 865, 931, 1014, 1066 |
| PleI | GAGTC | 1 cut(s) | 990 |
| PpsI | GAGTC | 1 cut(s) | 990 |
| PscI | ACATGT | 2 cut(s) | 496, 1138 |
| PshBI | ATTAAT | 1 cut(s) | 345 |
| PsiI | TTATAA | 1 cut(s) | 791 |
| Psp124BI | GAGCTC | 1 cut(s) | 1120 |
| Psp6I | CCWGG | 1 cut(s) | 651 |
| PspGI | CCWGG | 1 cut(s) | 651 |
| PspN4I | GGNNCC | 2 cut(s) | 188, 1029 |
| PspPI | GGNCC | 1 cut(s) | 1028 |
| PsyI | GACNNNGTC | 1 cut(s) | 11 |
| PvuII | CAGCTG | 2 cut(s) | 1015, 1067 |
| RsaI | GTAC | 3 cut(s) | 549, 738, 1197 |
| RsaNI | GTAC | 3 cut(s) | 548, 737, 1196 |
| RseI | CAYNNNNRTG | 1 cut(s) | 501 |
| SacI | GAGCTC | 1 cut(s) | 1120 |
| SaqAI | TTAA | 5 cut(s) | 345, 593, 648, 959, 1151 |
| SatI | GCNGC | 6 cut(s) | 62, 848, 864, 930, 1013, 1065 |
| Sau3AI | GATC | 3 cut(s) | 666, 710, 1189 |
| Sau96I | GGNCC | 1 cut(s) | 1028 |
| SchI | GAGTC | 1 cut(s) | 990 |
| ScrFI | CCNGG | 1 cut(s) | 653 |
| SduI | GDGCHC | 2 cut(s) | 949, 1120 |
| SfaNI | GCATC | 3 cut(s) | 133, 886, 986 |
| SfcI | CTRYAG | 2 cut(s) | 1308, 1325 |
| Sfr274I | CTCGAG | 1 cut(s) | 521 |
| SinI | GGWCC | 1 cut(s) | 1028 |
| SlaI | CTCGAG | 1 cut(s) | 521 |
| SmiI | ATTTAAAT | 1 cut(s) | 960 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 501 |
| SmlI | CTYRAG | 3 cut(s) | 521, 551, 1088 |
| SmoI | CTYRAG | 3 cut(s) | 521, 551, 1088 |
| SpeI | ACTAGT | 1 cut(s) | 922 |
| SsiI | CCGC | 4 cut(s) | 55, 62, 573, 863 |
| SspMI | CTAG | 4 cut(s) | 96, 420, 923, 1263 |
| SstI | GAGCTC | 1 cut(s) | 1120 |
| StyD4I | CCNGG | 1 cut(s) | 651 |
| SwaI | ATTTAAAT | 1 cut(s) | 960 |
| TaaI | ACNGT | 5 cut(s) | 120, 377, 542, 760, 1309 |
| TaqI | TCGA | 5 cut(s) | 522, 713, 855, 1114, 1245 |
| TauI | GCSGC | 2 cut(s) | 64, 866 |
| TfiI | GAWTC | 3 cut(s) | 241, 355, 838 |
| Tru1I | TTAA | 5 cut(s) | 345, 593, 648, 959, 1151 |
| Tru9I | TTAA | 5 cut(s) | 345, 593, 648, 959, 1151 |
| TscAI | CASTG | 6 cut(s) | 22, 91, 382, 570, 776, 1176 |
| TseFI | GTSAC | 2 cut(s) | 505, 565 |
| TseI | GCWGC | 4 cut(s) | 847, 929, 1012, 1064 |
| Tsp45I | GTSAC | 2 cut(s) | 505, 565 |
| TspDTI | ATGAA | 3 cut(s) | 363, 441, 941 |
| TspGWI | ACGGA | 1 cut(s) | 1213 |
| TspRI | CASTG | 6 cut(s) | 22, 91, 382, 570, 776, 1176 |
| Tth111I | GACNNNGTC | 1 cut(s) | 11 |
| VpaK11BI | GGWCC | 1 cut(s) | 1028 |
| VspI | ATTAAT | 1 cut(s) | 345 |
| XapI | RAATTY | 4 cut(s) | 200, 299, 589, 1313 |
| XceI | RCATGY | 2 cut(s) | 500, 1142 |
| XhoI | CTCGAG | 1 cut(s) | 521 |
| XmiI | GTMKAC | 1 cut(s) | 122 |
| XmnI | GAANNNNTTC | 1 cut(s) | 354 |
| XspI | CTAG | 4 cut(s) | 96, 420, 923, 1263 |