diphthine-ammonia ligase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 773 |
| Acc36I | ACCTGC | 1 cut(s) | 711 |
| AccBSI | CCGCTC | 1 cut(s) | 845 |
| AccI | GTMKAC | 1 cut(s) | 122 |
| AciI | CCGC | 4 cut(s) | 55, 62, 555, 845 |
| AclWI | GGATC | 1 cut(s) | 1166 |
| AcsI | RAATTY | 4 cut(s) | 200, 281, 571, 1295 |
| AcuI | CTGAAG | 5 cut(s) | 452, 845, 908, 974, 1269 |
| AfaI | GTAC | 3 cut(s) | 531, 720, 1179 |
| AflIII | ACRYGT | 2 cut(s) | 478, 1120 |
| AgsI | TTSAA | 9 cut(s) | 318, 341, 365, 374, 451, 604, 988, 1118, 1271 |
| AhlI | ACTAGT | 1 cut(s) | 904 |
| AjnI | CCWGG | 1 cut(s) | 633 |
| Alw21I | GWGCWC | 1 cut(s) | 1102 |
| Alw26I | GTCTC | 2 cut(s) | 648, 970 |
| AlwI | GGATC | 1 cut(s) | 1166 |
| Ama87I | CYCGRG | 1 cut(s) | 503 |
| AoxI | GGCC | 3 cut(s) | 846, 1211, 1242 |
| ApeKI | GCWGC | 4 cut(s) | 829, 911, 994, 1046 |
| ApoI | RAATTY | 4 cut(s) | 200, 281, 571, 1295 |
| AseI | ATTAAT | 1 cut(s) | 327 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 336 |
| AspLEI | GCGC | 1 cut(s) | 931 |
| AspS9I | GGNCC | 1 cut(s) | 1010 |
| AsuHPI | GGTGA | 3 cut(s) | 250, 625, 1058 |
| AvaI | CYCGRG | 1 cut(s) | 503 |
| AvaII | GGWCC | 1 cut(s) | 1010 |
| AxyI | CCTNAGG | 1 cut(s) | 134 |
| BanII | GRGCYC | 1 cut(s) | 1102 |
| BbsI | GAAGAC | 1 cut(s) | 417 |
| Bbv12I | GWGCWC | 1 cut(s) | 1102 |
| BbvI | GCAGC | 4 cut(s) | 816, 923, 1006, 1058 |
| BccI | CCATC | 2 cut(s) | 96, 1021 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 685, 719 |
| BciT130I | CCWGG | 1 cut(s) | 635 |
| BcoDI | GTCTC | 2 cut(s) | 648, 970 |
| BcuI | ACTAGT | 1 cut(s) | 904 |
| BfaI | CTAG | 3 cut(s) | 96, 402, 905 |
| BfmI | CTRYAG | 2 cut(s) | 75, 1307 |
| BfoI | RGCGCY | 1 cut(s) | 932 |
| BfuAI | ACCTGC | 1 cut(s) | 711 |
| BisI | GCNGC | 6 cut(s) | 62, 830, 846, 912, 995, 1047 |
| BlpI | GCTNAGC | 1 cut(s) | 1257 |
| BlsI | GCNGC | 6 cut(s) | 63, 831, 847, 913, 996, 1048 |
| Bme1390I | CCNGG | 1 cut(s) | 635 |
| Bme18I | GGWCC | 1 cut(s) | 1010 |
| BmeT110I | CYCGRG | 1 cut(s) | 503 |
| BmgT120I | GGNCC | 1 cut(s) | 1010 |
| BmiI | GGNNCC | 1 cut(s) | 1011 |
| BmrFI | CCNGG | 1 cut(s) | 635 |
| BmsI | GCATC | 2 cut(s) | 133, 968 |
| BpiI | GAAGAC | 1 cut(s) | 417 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1180, 1212 |
| BpmI | CTGGAG | 1 cut(s) | 1175 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1257 |
| BpuEI | CTTGAG | 3 cut(s) | 554, 1055, 1182 |
| BsaBI | GATNNNNATC | 2 cut(s) | 384, 691 |
| BsaWI | WCCGGW | 1 cut(s) | 526 |
| Bse1I | ACTGG | 3 cut(s) | 84, 496, 1158 |
| Bse21I | CCTNAGG | 1 cut(s) | 134 |
| Bse3DI | GCAATG | 3 cut(s) | 20, 354, 722 |
| Bse8I | GATNNNNATC | 2 cut(s) | 384, 691 |
| BseBI | CCWGG | 1 cut(s) | 635 |
| BseJI | GATNNNNATC | 2 cut(s) | 384, 691 |
| BseMI | GCAATG | 3 cut(s) | 20, 354, 722 |
| BseMII | CTCAG | 1 cut(s) | 723 |
| BseNI | ACTGG | 3 cut(s) | 84, 496, 1158 |
| BseXI | GCAGC | 4 cut(s) | 816, 923, 1006, 1058 |
| BshFI | GGCC | 3 cut(s) | 848, 1213, 1244 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1102 |
| BsiHKCI | CYCGRG | 1 cut(s) | 503 |
| BsiSI | CCGG | 1 cut(s) | 527 |
| BslFI | GGGAC | 2 cut(s) | 37, 129 |
| BsmAI | GTCTC | 2 cut(s) | 648, 970 |
| BsmBI | CGTCTC | 1 cut(s) | 648 |
| BsmFI | GGGAC | 2 cut(s) | 37, 129 |
| BsnI | GGCC | 3 cut(s) | 848, 1213, 1244 |
| BsoBI | CYCGRG | 1 cut(s) | 503 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1102 |
| Bsp143I | GATC | 4 cut(s) | 648, 692, 1171, 1249 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1257 |
| BspACI | CCGC | 4 cut(s) | 55, 62, 555, 845 |
| BspANI | GGCC | 3 cut(s) | 848, 1213, 1244 |
| BspCNI | CTCAG | 1 cut(s) | 724 |
| BspLI | GGNNCC | 1 cut(s) | 1011 |
| BspMI | ACCTGC | 1 cut(s) | 711 |
| BspPI | GGATC | 1 cut(s) | 1166 |
| BsrBI | CCGCTC | 1 cut(s) | 845 |
| BsrDI | GCAATG | 3 cut(s) | 20, 354, 722 |
| BsrI | ACTGG | 3 cut(s) | 84, 496, 1158 |
| BssMI | GATC | 4 cut(s) | 648, 692, 1171, 1249 |
| Bst2UI | CCWGG | 1 cut(s) | 635 |
| Bst4CI | ACNGT | 4 cut(s) | 120, 359, 524, 742 |
| BstC8I | GCNNGC | 1 cut(s) | 59 |
| BstDEI | CTNAG | 4 cut(s) | 134, 732, 747, 1257 |
| BstH2I | RGCGCY | 1 cut(s) | 932 |
| BstHHI | GCGC | 1 cut(s) | 931 |
| BstKTI | GATC | 4 cut(s) | 651, 695, 1174, 1252 |
| BstMAI | GTCTC | 2 cut(s) | 648, 970 |
| BstMBI | GATC | 4 cut(s) | 648, 692, 1171, 1249 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 757 |
| BstNI | CCWGG | 1 cut(s) | 635 |
| BstNSI | RCATGY | 2 cut(s) | 482, 1124 |
| BstSCI | CCNGG | 1 cut(s) | 633 |
| BstSFI | CTRYAG | 2 cut(s) | 75, 1307 |
| BstV1I | GCAGC | 4 cut(s) | 816, 923, 1006, 1058 |
| BstV2I | GAAGAC | 1 cut(s) | 417 |
| Bsu36I | CCTNAGG | 1 cut(s) | 134 |
| BsuRI | GGCC | 3 cut(s) | 848, 1213, 1244 |
| BtgZI | GCGATG | 1 cut(s) | 115 |
| BtsI | GCAGTG | 1 cut(s) | 758 |
| BtsIMutI | CAGTG | 6 cut(s) | 22, 91, 364, 552, 758, 1151 |
| BveI | ACCTGC | 1 cut(s) | 711 |
| Cac8I | GCNNGC | 1 cut(s) | 59 |
| CfoI | GCGC | 1 cut(s) | 931 |
| Cfr13I | GGNCC | 1 cut(s) | 1010 |
| Csp6I | GTAC | 3 cut(s) | 530, 719, 1178 |
| CviAII | CATG | 8 cut(s) | 245, 479, 1017, 1021, 1121, 1126, 1185, 1253 |
| CviQI | GTAC | 3 cut(s) | 530, 719, 1178 |
| DdeI | CTNAG | 4 cut(s) | 134, 732, 747, 1257 |
| DpnI | GATC | 4 cut(s) | 650, 694, 1173, 1251 |
| DpnII | GATC | 4 cut(s) | 648, 692, 1171, 1249 |
| DraI | TTTAAA | 2 cut(s) | 205, 942 |
| Ecl136II | GAGCTC | 1 cut(s) | 1100 |
| Eco24I | GRGCYC | 1 cut(s) | 1102 |
| Eco32I | GATATC | 1 cut(s) | 1193 |
| Eco47I | GGWCC | 1 cut(s) | 1010 |
| Eco53kI | GAGCTC | 1 cut(s) | 1100 |
| Eco57I | CTGAAG | 5 cut(s) | 452, 845, 908, 974, 1269 |
| Eco81I | CCTNAGG | 1 cut(s) | 134 |
| Eco88I | CYCGRG | 1 cut(s) | 503 |
| EcoICRI | GAGCTC | 1 cut(s) | 1100 |
| EcoRI | GAATTC | 1 cut(s) | 281 |
| EcoRII | CCWGG | 1 cut(s) | 633 |
| EcoRV | GATATC | 1 cut(s) | 1193 |
| EcoT22I | ATGCAT | 1 cut(s) | 1022 |
| EcoT38I | GRGCYC | 1 cut(s) | 1102 |
| Esp3I | CGTCTC | 1 cut(s) | 648 |
| FaeI | CATG | 8 cut(s) | 248, 482, 1020, 1024, 1124, 1129, 1188, 1256 |
| FaqI | GGGAC | 2 cut(s) | 37, 129 |
| FatI | CATG | 8 cut(s) | 244, 478, 1016, 1020, 1120, 1125, 1184, 1252 |
| FauI | CCCGC | 1 cut(s) | 62 |
| FblI | GTMKAC | 1 cut(s) | 122 |
| Fnu4HI | GCNGC | 6 cut(s) | 62, 830, 846, 912, 995, 1047 |
| FriOI | GRGCYC | 1 cut(s) | 1102 |
| Fsp4HI | GCNGC | 6 cut(s) | 62, 830, 846, 912, 995, 1047 |
| FspBI | CTAG | 3 cut(s) | 96, 402, 905 |
| GlaI | GCGC | 1 cut(s) | 930 |
| GluI | GCNGC | 6 cut(s) | 62, 830, 846, 912, 995, 1047 |
| GsuI | CTGGAG | 1 cut(s) | 1175 |
| HaeII | RGCGCY | 1 cut(s) | 932 |
| HaeIII | GGCC | 3 cut(s) | 848, 1213, 1244 |
| HapII | CCGG | 1 cut(s) | 527 |
| HhaI | GCGC | 1 cut(s) | 931 |
| Hin1II | CATG | 8 cut(s) | 248, 482, 1020, 1024, 1124, 1129, 1188, 1256 |
| Hin6I | GCGC | 1 cut(s) | 929 |
| HinP1I | GCGC | 1 cut(s) | 929 |
| HinfI | GANTC | 5 cut(s) | 241, 337, 820, 859, 978 |
| HpaII | CCGG | 1 cut(s) | 527 |
| HphI | GGTGA | 3 cut(s) | 250, 625, 1058 |
| Hpy166II | GTNNAC | 3 cut(s) | 7, 123, 661 |
| Hpy188I | TCNGA | 5 cut(s) | 476, 653, 733, 825, 888 |
| Hpy188III | TCNNGA | 4 cut(s) | 286, 503, 952, 1004 |
| Hpy8I | GTNNAC | 3 cut(s) | 7, 123, 661 |
| HpyAV | CCTTC | 2 cut(s) | 949, 1293 |
| HpyCH4III | ACNGT | 4 cut(s) | 120, 359, 524, 742 |
| HpyCH4IV | ACGT | 1 cut(s) | 657 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 757 |
| HpyF3I | CTNAG | 4 cut(s) | 134, 732, 747, 1257 |
| HpySE526I | ACGT | 1 cut(s) | 657 |
| Hsp92II | CATG | 8 cut(s) | 248, 482, 1020, 1024, 1124, 1129, 1188, 1256 |
| HspAI | GCGC | 1 cut(s) | 929 |
| Kzo9I | GATC | 4 cut(s) | 648, 692, 1171, 1249 |
| LmnI | GCTCC | 2 cut(s) | 477, 842 |
| Lsp1109I | GCAGC | 4 cut(s) | 816, 923, 1006, 1058 |
| LweI | GCATC | 2 cut(s) | 133, 968 |
| MaeI | CTAG | 3 cut(s) | 96, 402, 905 |
| MaeII | ACGT | 1 cut(s) | 657 |
| MaeIII | GTNAC | 3 cut(s) | 487, 547, 736 |
| MalI | GATC | 4 cut(s) | 650, 694, 1173, 1251 |
| MbiI | CCGCTC | 1 cut(s) | 845 |
| MboI | GATC | 4 cut(s) | 648, 692, 1171, 1249 |
| MboII | GAAGA | 3 cut(s) | 290, 422, 458 |
| MfeI | CAATTG | 1 cut(s) | 232 |
| MhlI | GDGCHC | 1 cut(s) | 1102 |
| MlyI | GAGTC | 1 cut(s) | 972 |
| MmeI | TCCRAC | 2 cut(s) | 499, 543 |
| MnlI | CCTC | 8 cut(s) | 257, 467, 727, 832, 882, 1000, 1079, 1206 |
| Mph1103I | ATGCAT | 1 cut(s) | 1022 |
| MroXI | GAANNNNTTC | 1 cut(s) | 336 |
| MseI | TTAA | 7 cut(s) | 204, 327, 575, 630, 941, 1133, 1230 |
| MslI | CAYNNNNRTG | 1 cut(s) | 483 |
| MspA1I | CMGCKG | 3 cut(s) | 997, 1049, 1289 |
| MspI | CCGG | 1 cut(s) | 527 |
| MspR9I | CCNGG | 1 cut(s) | 635 |
| MunI | CAATTG | 1 cut(s) | 232 |
| MvaI | CCWGG | 1 cut(s) | 635 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 757 |
| NdeII | GATC | 4 cut(s) | 648, 692, 1171, 1249 |
| NlaIII | CATG | 8 cut(s) | 248, 482, 1020, 1024, 1124, 1129, 1188, 1256 |
| NlaIV | GGNNCC | 1 cut(s) | 1011 |
| NmuCI | GTSAC | 2 cut(s) | 487, 547 |
| NsiI | ATGCAT | 1 cut(s) | 1022 |
| NspI | RCATGY | 2 cut(s) | 482, 1124 |
| PaeR7I | CTCGAG | 1 cut(s) | 503 |
| PciI | ACATGT | 2 cut(s) | 478, 1120 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 596, 663 |
| PdmI | GAANNNNTTC | 1 cut(s) | 336 |
| PfeI | GAWTC | 4 cut(s) | 241, 337, 820, 859 |
| PflFI | GACNNNGTC | 1 cut(s) | 11 |
| PkrI | GCNGC | 6 cut(s) | 63, 831, 847, 913, 996, 1048 |
| PleI | GAGTC | 1 cut(s) | 972 |
| PpsI | GAGTC | 1 cut(s) | 972 |
| PscI | ACATGT | 2 cut(s) | 478, 1120 |
| PshBI | ATTAAT | 1 cut(s) | 327 |
| PsiI | TTATAA | 1 cut(s) | 773 |
| Psp124BI | GAGCTC | 1 cut(s) | 1102 |
| Psp6I | CCWGG | 1 cut(s) | 633 |
| PspGI | CCWGG | 1 cut(s) | 633 |
| PspN4I | GGNNCC | 1 cut(s) | 1011 |
| PspPI | GGNCC | 1 cut(s) | 1010 |
| PsyI | GACNNNGTC | 1 cut(s) | 11 |
| PvuII | CAGCTG | 3 cut(s) | 997, 1049, 1289 |
| RsaI | GTAC | 3 cut(s) | 531, 720, 1179 |
| RsaNI | GTAC | 3 cut(s) | 530, 719, 1178 |
| RseI | CAYNNNNRTG | 1 cut(s) | 483 |
| SacI | GAGCTC | 1 cut(s) | 1102 |
| SaqAI | TTAA | 7 cut(s) | 204, 327, 575, 630, 941, 1133, 1230 |
| SatI | GCNGC | 6 cut(s) | 62, 830, 846, 912, 995, 1047 |
| Sau3AI | GATC | 4 cut(s) | 648, 692, 1171, 1249 |
| Sau96I | GGNCC | 1 cut(s) | 1010 |
| SchI | GAGTC | 1 cut(s) | 972 |
| ScrFI | CCNGG | 1 cut(s) | 635 |
| SduI | GDGCHC | 1 cut(s) | 1102 |
| SfaNI | GCATC | 2 cut(s) | 133, 968 |
| SfcI | CTRYAG | 2 cut(s) | 75, 1307 |
| Sfr274I | CTCGAG | 1 cut(s) | 503 |
| SinI | GGWCC | 1 cut(s) | 1010 |
| SlaI | CTCGAG | 1 cut(s) | 503 |
| SmiI | ATTTAAAT | 1 cut(s) | 942 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 483 |
| SmlI | CTYRAG | 4 cut(s) | 503, 533, 1070, 1197 |
| SmoI | CTYRAG | 4 cut(s) | 503, 533, 1070, 1197 |
| SpeI | ACTAGT | 1 cut(s) | 904 |
| SsiI | CCGC | 4 cut(s) | 55, 62, 555, 845 |
| SspMI | CTAG | 3 cut(s) | 96, 402, 905 |
| SstI | GAGCTC | 1 cut(s) | 1102 |
| StyD4I | CCNGG | 1 cut(s) | 633 |
| SwaI | ATTTAAAT | 1 cut(s) | 942 |
| TaaI | ACNGT | 4 cut(s) | 120, 359, 524, 742 |
| TaiI | ACGT | 1 cut(s) | 660 |
| TaqI | TCGA | 4 cut(s) | 504, 695, 837, 1096 |
| TauI | GCSGC | 2 cut(s) | 64, 848 |
| TfiI | GAWTC | 4 cut(s) | 241, 337, 820, 859 |
| Tru1I | TTAA | 7 cut(s) | 204, 327, 575, 630, 941, 1133, 1230 |
| Tru9I | TTAA | 7 cut(s) | 204, 327, 575, 630, 941, 1133, 1230 |
| TscAI | CASTG | 6 cut(s) | 22, 91, 364, 552, 758, 1158 |
| TseFI | GTSAC | 2 cut(s) | 487, 547 |
| TseI | GCWGC | 4 cut(s) | 829, 911, 994, 1046 |
| Tsp45I | GTSAC | 2 cut(s) | 487, 547 |
| TspDTI | ATGAA | 3 cut(s) | 345, 423, 923 |
| TspGWI | ACGGA | 1 cut(s) | 1195 |
| TspRI | CASTG | 6 cut(s) | 22, 91, 364, 552, 758, 1158 |
| Tth111I | GACNNNGTC | 1 cut(s) | 11 |
| VpaK11BI | GGWCC | 1 cut(s) | 1010 |
| VspI | ATTAAT | 1 cut(s) | 327 |
| XapI | RAATTY | 4 cut(s) | 200, 281, 571, 1295 |
| XceI | RCATGY | 2 cut(s) | 482, 1124 |
| XhoI | CTCGAG | 1 cut(s) | 503 |
| XmiI | GTMKAC | 1 cut(s) | 122 |
| XmnI | GAANNNNTTC | 1 cut(s) | 336 |
| XspI | CTAG | 3 cut(s) | 96, 402, 905 |
| Zsp2I | ATGCAT | 1 cut(s) | 1022 |