diphthine-ammonia ligase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 806, 960 |
| Acc16I | TGCGCA | 1 cut(s) | 595 |
| AccBSI | CCGCTC | 1 cut(s) | 881 |
| AccI | GTMKAC | 1 cut(s) | 122 |
| AciI | CCGC | 4 cut(s) | 55, 62, 175, 881 |
| AclWI | GGATC | 4 cut(s) | 299, 452, 505, 1199 |
| AcsI | RAATTY | 2 cut(s) | 284, 1328 |
| AcuI | CTGAAG | 3 cut(s) | 491, 878, 944 |
| AfaI | GTAC | 3 cut(s) | 212, 753, 1212 |
| AfeI | AGCGCT | 1 cut(s) | 221 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 461 |
| AflIII | ACRYGT | 1 cut(s) | 517 |
| AhlI | ACTAGT | 1 cut(s) | 940 |
| AjnI | CCWGG | 1 cut(s) | 672 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 812, 844 |
| AluBI | AGCT | 9 cut(s) | 80, 203, 862, 874, 984, 1033, 1082, 1133, 1238 |
| AluI | AGCT | 9 cut(s) | 80, 203, 862, 874, 984, 1033, 1082, 1133, 1238 |
| Alw21I | GWGCWC | 1 cut(s) | 1135 |
| Alw26I | GTCTC | 3 cut(s) | 687, 1006, 1216 |
| AlwI | GGATC | 4 cut(s) | 299, 452, 505, 1199 |
| Ama87I | CYCGRG | 2 cut(s) | 542, 572 |
| Aor51HI | AGCGCT | 1 cut(s) | 221 |
| AoxI | GGCC | 1 cut(s) | 883 |
| ApeKI | GCWGC | 3 cut(s) | 862, 1030, 1079 |
| ApoI | RAATTY | 2 cut(s) | 284, 1328 |
| AseI | ATTAAT | 1 cut(s) | 330 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 339 |
| AspLEI | GCGC | 2 cut(s) | 222, 596 |
| AspS9I | GGNCC | 1 cut(s) | 883 |
| AsuHPI | GGTGA | 3 cut(s) | 253, 664, 1091 |
| AvaI | CYCGRG | 2 cut(s) | 542, 572 |
| AxyI | CCTNAGG | 1 cut(s) | 134 |
| BanII | GRGCYC | 1 cut(s) | 1135 |
| Bbv12I | GWGCWC | 1 cut(s) | 1135 |
| BbvI | GCAGC | 3 cut(s) | 849, 1042, 1091 |
| BccI | CCATC | 1 cut(s) | 96 |
| BceAI | ACGGC | 1 cut(s) | 169 |
| BciT130I | CCWGG | 1 cut(s) | 674 |
| BciVI | GTATCC | 1 cut(s) | 467 |
| BcoDI | GTCTC | 3 cut(s) | 687, 1006, 1216 |
| BcuI | ACTAGT | 1 cut(s) | 940 |
| BfaI | CTAG | 4 cut(s) | 96, 392, 405, 941 |
| BfmI | CTRYAG | 3 cut(s) | 204, 1323, 1340 |
| BfoI | RGCGCY | 1 cut(s) | 223 |
| BfuI | GTATCC | 1 cut(s) | 467 |
| BisI | GCNGC | 4 cut(s) | 62, 863, 1031, 1080 |
| BlpI | GCTNAGC | 1 cut(s) | 1290 |
| BlsI | GCNGC | 4 cut(s) | 63, 864, 1032, 1081 |
| BmcAI | AGTACT | 1 cut(s) | 212 |
| Bme1390I | CCNGG | 1 cut(s) | 674 |
| BmeT110I | CYCGRG | 2 cut(s) | 542, 572 |
| BmgT120I | GGNCC | 1 cut(s) | 883 |
| BmrFI | CCNGG | 1 cut(s) | 674 |
| BmsI | GCATC | 3 cut(s) | 133, 874, 1004 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 525, 557 |
| BpmI | CTGGAG | 3 cut(s) | 552, 854, 1208 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1290 |
| BpuEI | CTTGAG | 3 cut(s) | 395, 974, 1088 |
| Bsa29I | ATCGAT | 2 cut(s) | 234, 632 |
| BsaBI | GATNNNNATC | 1 cut(s) | 387 |
| BsaJI | CCNNGG | 1 cut(s) | 185 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 461 |
| Bse1I | ACTGG | 4 cut(s) | 84, 218, 535, 1191 |
| Bse21I | CCTNAGG | 1 cut(s) | 134 |
| Bse3DI | GCAATG | 3 cut(s) | 20, 357, 755 |
| Bse8I | GATNNNNATC | 1 cut(s) | 387 |
| BseBI | CCWGG | 1 cut(s) | 674 |
| BseCI | ATCGAT | 2 cut(s) | 234, 632 |
| BseDI | CCNNGG | 1 cut(s) | 185 |
| BseGI | GGATG | 1 cut(s) | 33 |
| BseJI | GATNNNNATC | 1 cut(s) | 387 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 461 |
| BseMI | GCAATG | 3 cut(s) | 20, 357, 755 |
| BseMII | CTCAG | 1 cut(s) | 756 |
| BseNI | ACTGG | 4 cut(s) | 84, 218, 535, 1191 |
| BseXI | GCAGC | 3 cut(s) | 849, 1042, 1091 |
| BseYI | CCCAGC | 1 cut(s) | 874 |
| BshFI | GGCC | 1 cut(s) | 885 |
| BshVI | ATCGAT | 2 cut(s) | 234, 632 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1135 |
| BsiHKCI | CYCGRG | 2 cut(s) | 542, 572 |
| BslFI | GGGAC | 3 cut(s) | 37, 129, 478 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 461 |
| BsmAI | GTCTC | 3 cut(s) | 687, 1006, 1216 |
| BsmBI | CGTCTC | 1 cut(s) | 687 |
| BsmFI | GGGAC | 3 cut(s) | 37, 129, 478 |
| BsnI | GGCC | 1 cut(s) | 885 |
| BsoBI | CYCGRG | 2 cut(s) | 542, 572 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1135 |
| Bsp143I | GATC | 6 cut(s) | 291, 388, 444, 510, 1204, 1282 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1290 |
| BspACI | CCGC | 4 cut(s) | 55, 62, 175, 881 |
| BspANI | GGCC | 1 cut(s) | 885 |
| BspCNI | CTCAG | 1 cut(s) | 757 |
| BspDI | ATCGAT | 2 cut(s) | 234, 632 |
| BspPI | GGATC | 4 cut(s) | 299, 452, 505, 1199 |
| BsrBI | CCGCTC | 1 cut(s) | 881 |
| BsrDI | GCAATG | 3 cut(s) | 20, 357, 755 |
| BsrI | ACTGG | 4 cut(s) | 84, 218, 535, 1191 |
| BssECI | CCNNGG | 1 cut(s) | 185 |
| BssMI | GATC | 6 cut(s) | 291, 388, 444, 510, 1204, 1282 |
| BssT1I | CCWWGG | 1 cut(s) | 185 |
| Bst2UI | CCWGG | 1 cut(s) | 674 |
| Bst4CI | ACNGT | 5 cut(s) | 120, 261, 563, 775, 1324 |
| BstC8I | GCNNGC | 3 cut(s) | 59, 883, 1051 |
| BstDEI | CTNAG | 3 cut(s) | 134, 765, 1290 |
| BstEII | GGTNACC | 1 cut(s) | 769 |
| BstF5I | GGATG | 1 cut(s) | 33 |
| BstH2I | RGCGCY | 1 cut(s) | 223 |
| BstHHI | GCGC | 2 cut(s) | 222, 596 |
| BstKTI | GATC | 6 cut(s) | 294, 391, 447, 513, 1207, 1285 |
| BstMAI | GTCTC | 3 cut(s) | 687, 1006, 1216 |
| BstMBI | GATC | 6 cut(s) | 291, 388, 444, 510, 1204, 1282 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 871 |
| BstNI | CCWGG | 1 cut(s) | 674 |
| BstNSI | RCATGY | 2 cut(s) | 521, 1053 |
| BstPI | GGTNACC | 1 cut(s) | 769 |
| BstSCI | CCNGG | 1 cut(s) | 672 |
| BstSFI | CTRYAG | 3 cut(s) | 204, 1323, 1340 |
| BstV1I | GCAGC | 3 cut(s) | 849, 1042, 1091 |
| BstX2I | RGATCY | 2 cut(s) | 444, 510 |
| BstYI | RGATCY | 2 cut(s) | 444, 510 |
| Bsu15I | ATCGAT | 2 cut(s) | 234, 632 |
| Bsu36I | CCTNAGG | 1 cut(s) | 134 |
| BsuI | GTATCC | 1 cut(s) | 467 |
| BsuRI | GGCC | 1 cut(s) | 885 |
| BsuTUI | ATCGAT | 2 cut(s) | 234, 632 |
| BtgZI | GCGATG | 1 cut(s) | 115 |
| BtsCI | GGATG | 1 cut(s) | 33 |
| BtsIMutI | CAGTG | 2 cut(s) | 22, 91 |
| Cac8I | GCNNGC | 3 cut(s) | 59, 883, 1051 |
| CfoI | GCGC | 2 cut(s) | 222, 596 |
| Cfr13I | GGNCC | 1 cut(s) | 883 |
| ClaI | ATCGAT | 2 cut(s) | 234, 632 |
| Csp6I | GTAC | 3 cut(s) | 211, 752, 1211 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 167, 202 |
| CviAII | CATG | 6 cut(s) | 518, 1050, 1054, 1159, 1218, 1286 |
| CviQI | GTAC | 3 cut(s) | 211, 752, 1211 |
| DdeI | CTNAG | 3 cut(s) | 134, 765, 1290 |
| DpnI | GATC | 6 cut(s) | 293, 390, 446, 512, 1206, 1284 |
| DpnII | GATC | 6 cut(s) | 291, 388, 444, 510, 1204, 1282 |
| DraI | TTTAAA | 1 cut(s) | 978 |
| EciI | GGCGGA | 1 cut(s) | 164 |
| Ecl136II | GAGCTC | 1 cut(s) | 1133 |
| Eco130I | CCWWGG | 1 cut(s) | 185 |
| Eco24I | GRGCYC | 1 cut(s) | 1135 |
| Eco32I | GATATC | 1 cut(s) | 728 |
| Eco47III | AGCGCT | 1 cut(s) | 221 |
| Eco53kI | GAGCTC | 1 cut(s) | 1133 |
| Eco57I | CTGAAG | 3 cut(s) | 491, 878, 944 |
| Eco81I | CCTNAGG | 1 cut(s) | 134 |
| Eco88I | CYCGRG | 2 cut(s) | 542, 572 |
| Eco91I | GGTNACC | 1 cut(s) | 769 |
| EcoICRI | GAGCTC | 1 cut(s) | 1133 |
| EcoO65I | GGTNACC | 1 cut(s) | 769 |
| EcoRI | GAATTC | 1 cut(s) | 284 |
| EcoRII | CCWGG | 1 cut(s) | 672 |
| EcoRV | GATATC | 1 cut(s) | 728 |
| EcoT14I | CCWWGG | 1 cut(s) | 185 |
| EcoT22I | ATGCAT | 1 cut(s) | 1055 |
| EcoT38I | GRGCYC | 1 cut(s) | 1135 |
| ErhI | CCWWGG | 1 cut(s) | 185 |
| Esp3I | CGTCTC | 1 cut(s) | 687 |
| FaeI | CATG | 6 cut(s) | 521, 1053, 1057, 1162, 1221, 1289 |
| FaqI | GGGAC | 3 cut(s) | 37, 129, 478 |
| FatI | CATG | 6 cut(s) | 517, 1049, 1053, 1158, 1217, 1285 |
| FauI | CCCGC | 2 cut(s) | 62, 874 |
| FblI | GTMKAC | 1 cut(s) | 122 |
| Fnu4HI | GCNGC | 4 cut(s) | 62, 863, 1031, 1080 |
| FokI | GGATG | 1 cut(s) | 20 |
| FriOI | GRGCYC | 1 cut(s) | 1135 |
| Fsp4HI | GCNGC | 4 cut(s) | 62, 863, 1031, 1080 |
| FspBI | CTAG | 4 cut(s) | 96, 392, 405, 941 |
| FspI | TGCGCA | 1 cut(s) | 595 |
| GlaI | GCGC | 2 cut(s) | 221, 595 |
| GluI | GCNGC | 4 cut(s) | 62, 863, 1031, 1080 |
| GsaI | CCCAGC | 1 cut(s) | 878 |
| GsuI | CTGGAG | 3 cut(s) | 552, 854, 1208 |
| HaeII | RGCGCY | 1 cut(s) | 223 |
| HaeIII | GGCC | 1 cut(s) | 885 |
| HhaI | GCGC | 2 cut(s) | 222, 596 |
| Hin1II | CATG | 6 cut(s) | 521, 1053, 1057, 1162, 1221, 1289 |
| Hin6I | GCGC | 2 cut(s) | 220, 594 |
| HinP1I | GCGC | 2 cut(s) | 220, 594 |
| HincII | GTYRAC | 1 cut(s) | 151 |
| HindII | GTYRAC | 1 cut(s) | 151 |
| HinfI | GANTC | 5 cut(s) | 244, 340, 853, 895, 1014 |
| HphI | GGTGA | 3 cut(s) | 253, 664, 1091 |
| Hpy166II | GTNNAC | 4 cut(s) | 7, 123, 151, 700 |
| Hpy188I | TCNGA | 7 cut(s) | 510, 515, 692, 766, 858, 924, 1042 |
| Hpy188III | TCNNGA | 3 cut(s) | 289, 542, 574 |
| Hpy8I | GTNNAC | 4 cut(s) | 7, 123, 151, 700 |
| HpyAV | CCTTC | 2 cut(s) | 1267, 1326 |
| HpyCH4III | ACNGT | 5 cut(s) | 120, 261, 563, 775, 1324 |
| HpyCH4IV | ACGT | 2 cut(s) | 696, 750 |
| HpyCH4V | TGCA | 9 cut(s) | 20, 256, 350, 865, 917, 1009, 1030, 1053, 1162 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 871 |
| HpyF3I | CTNAG | 3 cut(s) | 134, 765, 1290 |
| HpySE526I | ACGT | 2 cut(s) | 696, 750 |
| Hsp92II | CATG | 6 cut(s) | 521, 1053, 1057, 1162, 1221, 1289 |
| HspAI | GCGC | 2 cut(s) | 220, 594 |
| Kzo9I | GATC | 6 cut(s) | 291, 388, 444, 510, 1204, 1282 |
| LmnI | GCTCC | 2 cut(s) | 878, 1235 |
| Lsp1109I | GCAGC | 3 cut(s) | 849, 1042, 1091 |
| LweI | GCATC | 3 cut(s) | 133, 874, 1004 |
| MaeI | CTAG | 4 cut(s) | 96, 392, 405, 941 |
| MaeII | ACGT | 2 cut(s) | 696, 750 |
| MaeIII | GTNAC | 4 cut(s) | 526, 586, 746, 769 |
| MalI | GATC | 6 cut(s) | 293, 390, 446, 512, 1206, 1284 |
| MbiI | CCGCTC | 1 cut(s) | 881 |
| MboI | GATC | 6 cut(s) | 291, 388, 444, 510, 1204, 1282 |
| MboII | GAAGA | 4 cut(s) | 293, 425, 497, 1277 |
| MflI | RGATCY | 2 cut(s) | 444, 510 |
| MhlI | GDGCHC | 1 cut(s) | 1135 |
| MlyI | GAGTC | 1 cut(s) | 1008 |
| MmeI | TCCRAC | 4 cut(s) | 360, 538, 582, 867 |
| MnlI | CCTC | 7 cut(s) | 260, 308, 506, 760, 918, 998, 1112 |
| Mph1103I | ATGCAT | 1 cut(s) | 1055 |
| MroXI | GAANNNNTTC | 1 cut(s) | 339 |
| MseI | TTAA | 5 cut(s) | 330, 609, 614, 977, 1166 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1058, 1230 |
| MspA1I | CMGCKG | 3 cut(s) | 874, 1033, 1082 |
| MspR9I | CCNGG | 1 cut(s) | 674 |
| MvaI | CCWGG | 1 cut(s) | 674 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 871 |
| NdeII | GATC | 6 cut(s) | 291, 388, 444, 510, 1204, 1282 |
| NlaIII | CATG | 6 cut(s) | 521, 1053, 1057, 1162, 1221, 1289 |
| NmuCI | GTSAC | 2 cut(s) | 526, 586 |
| NsbI | TGCGCA | 1 cut(s) | 595 |
| NsiI | ATGCAT | 1 cut(s) | 1055 |
| NspI | RCATGY | 2 cut(s) | 521, 1053 |
| PaeI | GCATGC | 1 cut(s) | 1053 |
| PaeR7I | CTCGAG | 2 cut(s) | 542, 572 |
| PciI | ACATGT | 1 cut(s) | 517 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 702 |
| PdmI | GAANNNNTTC | 1 cut(s) | 339 |
| PfeI | GAWTC | 4 cut(s) | 244, 340, 853, 895 |
| PflFI | GACNNNGTC | 1 cut(s) | 11 |
| PkrI | GCNGC | 4 cut(s) | 63, 864, 1032, 1081 |
| PleI | GAGTC | 1 cut(s) | 1008 |
| PpsI | GAGTC | 1 cut(s) | 1008 |
| PscI | ACATGT | 1 cut(s) | 517 |
| PshBI | ATTAAT | 1 cut(s) | 330 |
| PsiI | TTATAA | 2 cut(s) | 806, 960 |
| Psp124BI | GAGCTC | 1 cut(s) | 1135 |
| Psp6I | CCWGG | 1 cut(s) | 672 |
| PspEI | GGTNACC | 1 cut(s) | 769 |
| PspFI | CCCAGC | 1 cut(s) | 874 |
| PspGI | CCWGG | 1 cut(s) | 672 |
| PspPI | GGNCC | 1 cut(s) | 883 |
| PsuI | RGATCY | 2 cut(s) | 444, 510 |
| PsyI | GACNNNGTC | 1 cut(s) | 11 |
| PvuII | CAGCTG | 3 cut(s) | 874, 1033, 1082 |
| RsaI | GTAC | 3 cut(s) | 212, 753, 1212 |
| RsaNI | GTAC | 3 cut(s) | 211, 752, 1211 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1058, 1230 |
| SacI | GAGCTC | 1 cut(s) | 1135 |
| SaqAI | TTAA | 5 cut(s) | 330, 609, 614, 977, 1166 |
| SatI | GCNGC | 4 cut(s) | 62, 863, 1031, 1080 |
| Sau3AI | GATC | 6 cut(s) | 291, 388, 444, 510, 1204, 1282 |
| Sau96I | GGNCC | 1 cut(s) | 883 |
| ScaI | AGTACT | 1 cut(s) | 212 |
| SchI | GAGTC | 1 cut(s) | 1008 |
| ScrFI | CCNGG | 1 cut(s) | 674 |
| SduI | GDGCHC | 1 cut(s) | 1135 |
| SfaNI | GCATC | 3 cut(s) | 133, 874, 1004 |
| SfcI | CTRYAG | 3 cut(s) | 204, 1323, 1340 |
| Sfr274I | CTCGAG | 2 cut(s) | 542, 572 |
| SlaI | CTCGAG | 2 cut(s) | 542, 572 |
| SmiI | ATTTAAAT | 1 cut(s) | 978 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1058, 1230 |
| SmlI | CTYRAG | 5 cut(s) | 374, 542, 572, 989, 1103 |
| SmoI | CTYRAG | 5 cut(s) | 374, 542, 572, 989, 1103 |
| SpeI | ACTAGT | 1 cut(s) | 940 |
| SphI | GCATGC | 1 cut(s) | 1053 |
| SsiI | CCGC | 4 cut(s) | 55, 62, 175, 881 |
| SspI | AATATT | 1 cut(s) | 481 |
| SspMI | CTAG | 4 cut(s) | 96, 392, 405, 941 |
| SstI | GAGCTC | 1 cut(s) | 1135 |
| StyD4I | CCNGG | 1 cut(s) | 672 |
| StyI | CCWWGG | 1 cut(s) | 185 |
| SwaI | ATTTAAAT | 1 cut(s) | 978 |
| TaaI | ACNGT | 5 cut(s) | 120, 261, 563, 775, 1324 |
| TaiI | ACGT | 2 cut(s) | 699, 753 |
| TaqI | TCGA | 7 cut(s) | 234, 366, 543, 573, 632, 1129, 1260 |
| TatI | WGTACW | 1 cut(s) | 210 |
| TauI | GCSGC | 1 cut(s) | 64 |
| TfiI | GAWTC | 4 cut(s) | 244, 340, 853, 895 |
| Tru1I | TTAA | 5 cut(s) | 330, 609, 614, 977, 1166 |
| Tru9I | TTAA | 5 cut(s) | 330, 609, 614, 977, 1166 |
| TscAI | CASTG | 2 cut(s) | 22, 91 |
| TseFI | GTSAC | 2 cut(s) | 526, 586 |
| TseI | GCWGC | 3 cut(s) | 862, 1030, 1079 |
| Tsp45I | GTSAC | 2 cut(s) | 526, 586 |
| TspDTI | ATGAA | 6 cut(s) | 143, 348, 618, 699, 959, 1278 |
| TspGWI | ACGGA | 1 cut(s) | 1228 |
| TspRI | CASTG | 2 cut(s) | 22, 91 |
| Tth111I | GACNNNGTC | 1 cut(s) | 11 |
| VspI | ATTAAT | 1 cut(s) | 330 |
| XapI | RAATTY | 2 cut(s) | 284, 1328 |
| XceI | RCATGY | 2 cut(s) | 521, 1053 |
| XhoI | CTCGAG | 2 cut(s) | 542, 572 |
| XmiI | GTMKAC | 1 cut(s) | 122 |
| XmnI | GAANNNNTTC | 1 cut(s) | 339 |
| XspI | CTAG | 4 cut(s) | 96, 392, 405, 941 |
| ZrmI | AGTACT | 1 cut(s) | 212 |
| Zsp2I | ATGCAT | 1 cut(s) | 1055 |