diphthine-ammonia ligase activity
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 747 |
| AccI | GTMKAC | 1 cut(s) | 122 |
| AciI | CCGC | 2 cut(s) | 62, 881 |
| AclWI | GGATC | 3 cut(s) | 449, 502, 1202 |
| AcsI | RAATTY | 2 cut(s) | 200, 281 |
| AcuI | CTGAAG | 4 cut(s) | 488, 881, 944, 1010 |
| AfaI | GTAC | 2 cut(s) | 756, 1215 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 458, 880, 1189 |
| AflIII | ACRYGT | 2 cut(s) | 514, 1156 |
| AhlI | ACTAGT | 1 cut(s) | 940 |
| AjnI | CCWGG | 1 cut(s) | 669 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1303, 1335 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1299, 1331 |
| AluBI | AGCT | 8 cut(s) | 80, 219, 294, 837, 865, 1033, 1085, 1136 |
| AluI | AGCT | 8 cut(s) | 80, 219, 294, 837, 865, 1033, 1085, 1136 |
| Alw21I | GWGCWC | 1 cut(s) | 1138 |
| Alw26I | GTCTC | 3 cut(s) | 684, 1006, 1278 |
| AlwI | GGATC | 3 cut(s) | 449, 502, 1202 |
| Ama87I | CYCGRG | 2 cut(s) | 539, 569 |
| AoxI | GGCC | 2 cut(s) | 882, 1247 |
| ApeKI | GCWGC | 4 cut(s) | 865, 947, 1030, 1082 |
| ApoI | RAATTY | 2 cut(s) | 200, 281 |
| AseI | ATTAAT | 1 cut(s) | 327 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 336 |
| AspS9I | GGNCC | 1 cut(s) | 1046 |
| AsuHPI | GGTGA | 4 cut(s) | 250, 661, 814, 1094 |
| AvaI | CYCGRG | 2 cut(s) | 539, 569 |
| AvaII | GGWCC | 1 cut(s) | 1046 |
| AxyI | CCTNAGG | 1 cut(s) | 134 |
| BaeGI | GKGCMC | 1 cut(s) | 967 |
| BanII | GRGCYC | 1 cut(s) | 1138 |
| Bbv12I | GWGCWC | 1 cut(s) | 1138 |
| BbvI | GCAGC | 4 cut(s) | 852, 959, 1042, 1094 |
| BccI | CCATC | 1 cut(s) | 96 |
| BciT130I | CCWGG | 1 cut(s) | 671 |
| BciVI | GTATCC | 1 cut(s) | 464 |
| BcoDI | GTCTC | 3 cut(s) | 684, 1006, 1278 |
| BcuI | ACTAGT | 1 cut(s) | 940 |
| BfaI | CTAG | 2 cut(s) | 96, 941 |
| BfmI | CTRYAG | 2 cut(s) | 1326, 1343 |
| BfuAI | ACCTGC | 1 cut(s) | 747 |
| BfuI | GTATCC | 1 cut(s) | 464 |
| BisI | GCNGC | 6 cut(s) | 62, 866, 882, 948, 1031, 1083 |
| BlpI | GCTNAGC | 1 cut(s) | 1293 |
| BlsI | GCNGC | 6 cut(s) | 63, 867, 883, 949, 1032, 1084 |
| Bme1390I | CCNGG | 1 cut(s) | 671 |
| Bme18I | GGWCC | 1 cut(s) | 1046 |
| BmeT110I | CYCGRG | 2 cut(s) | 539, 569 |
| BmgT120I | GGNCC | 1 cut(s) | 1046 |
| BmiI | GGNNCC | 1 cut(s) | 1047 |
| BmrFI | CCNGG | 1 cut(s) | 671 |
| BmsI | GCATC | 3 cut(s) | 133, 904, 1004 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 522, 554 |
| BpmI | CTGGAG | 2 cut(s) | 549, 1211 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1293 |
| BpuEI | CTTGAG | 1 cut(s) | 1091 |
| BsaBI | GATNNNNATC | 1 cut(s) | 384 |
| BsaI | GGTCTC | 1 cut(s) | 1278 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 458, 880, 1189 |
| Bse1I | ACTGG | 3 cut(s) | 84, 532, 1194 |
| Bse21I | CCTNAGG | 1 cut(s) | 134 |
| Bse3DI | GCAATG | 4 cut(s) | 20, 354, 758, 902 |
| Bse8I | GATNNNNATC | 1 cut(s) | 384 |
| BseBI | CCWGG | 1 cut(s) | 671 |
| BseGI | GGATG | 1 cut(s) | 895 |
| BseJI | GATNNNNATC | 1 cut(s) | 384 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 458, 880, 1189 |
| BseMI | GCAATG | 4 cut(s) | 20, 354, 758, 902 |
| BseMII | CTCAG | 1 cut(s) | 759 |
| BseNI | ACTGG | 3 cut(s) | 84, 532, 1194 |
| BseSI | GKGCMC | 1 cut(s) | 967 |
| BseXI | GCAGC | 4 cut(s) | 852, 959, 1042, 1094 |
| BsgI | GTGCAG | 1 cut(s) | 430 |
| BshFI | GGCC | 2 cut(s) | 884, 1249 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1138 |
| BsiHKCI | CYCGRG | 2 cut(s) | 539, 569 |
| BslFI | GGGAC | 2 cut(s) | 129, 475 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 458, 880, 1189 |
| BsmAI | GTCTC | 3 cut(s) | 684, 1006, 1278 |
| BsmBI | CGTCTC | 1 cut(s) | 684 |
| BsmFI | GGGAC | 2 cut(s) | 129, 475 |
| BsnI | GGCC | 2 cut(s) | 884, 1249 |
| Bso31I | GGTCTC | 1 cut(s) | 1278 |
| BsoBI | CYCGRG | 2 cut(s) | 539, 569 |
| Bsp1286I | GDGCHC | 2 cut(s) | 967, 1138 |
| Bsp143I | GATC | 3 cut(s) | 441, 507, 1207 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1293 |
| BspACI | CCGC | 2 cut(s) | 62, 881 |
| BspANI | GGCC | 2 cut(s) | 884, 1249 |
| BspCNI | CTCAG | 1 cut(s) | 760 |
| BspLI | GGNNCC | 1 cut(s) | 1047 |
| BspMI | ACCTGC | 1 cut(s) | 747 |
| BspPI | GGATC | 3 cut(s) | 449, 502, 1202 |
| BspTNI | GGTCTC | 1 cut(s) | 1278 |
| BsrDI | GCAATG | 4 cut(s) | 20, 354, 758, 902 |
| BsrI | ACTGG | 3 cut(s) | 84, 532, 1194 |
| BssMI | GATC | 3 cut(s) | 441, 507, 1207 |
| Bst2UI | CCWGG | 1 cut(s) | 671 |
| Bst4CI | ACNGT | 4 cut(s) | 120, 359, 778, 1327 |
| BstC8I | GCNNGC | 3 cut(s) | 59, 879, 1054 |
| BstDEI | CTNAG | 4 cut(s) | 134, 768, 783, 1293 |
| BstEII | GGTNACC | 1 cut(s) | 772 |
| BstF5I | GGATG | 1 cut(s) | 895 |
| BstKTI | GATC | 3 cut(s) | 444, 510, 1210 |
| BstMAI | GTCTC | 3 cut(s) | 684, 1006, 1278 |
| BstMBI | GATC | 3 cut(s) | 441, 507, 1207 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 874 |
| BstNI | CCWGG | 1 cut(s) | 671 |
| BstNSI | RCATGY | 4 cut(s) | 61, 518, 1056, 1160 |
| BstPI | GGTNACC | 1 cut(s) | 772 |
| BstSCI | CCNGG | 1 cut(s) | 669 |
| BstSFI | CTRYAG | 2 cut(s) | 1326, 1343 |
| BstSLI | GKGCMC | 1 cut(s) | 967 |
| BstV1I | GCAGC | 4 cut(s) | 852, 959, 1042, 1094 |
| BstX2I | RGATCY | 2 cut(s) | 441, 507 |
| BstYI | RGATCY | 2 cut(s) | 441, 507 |
| Bsu36I | CCTNAGG | 1 cut(s) | 134 |
| BsuI | GTATCC | 1 cut(s) | 464 |
| BsuRI | GGCC | 2 cut(s) | 884, 1249 |
| BtgZI | GCGATG | 1 cut(s) | 115 |
| BtsCI | GGATG | 1 cut(s) | 895 |
| BtsIMutI | CAGTG | 5 cut(s) | 22, 91, 364, 588, 1187 |
| BveI | ACCTGC | 1 cut(s) | 747 |
| Cac8I | GCNNGC | 3 cut(s) | 59, 879, 1054 |
| Cfr13I | GGNCC | 1 cut(s) | 1046 |
| Csp6I | GTAC | 2 cut(s) | 755, 1214 |
| CviQI | GTAC | 2 cut(s) | 755, 1214 |
| DdeI | CTNAG | 4 cut(s) | 134, 768, 783, 1293 |
| DpnI | GATC | 3 cut(s) | 443, 509, 1209 |
| DpnII | GATC | 3 cut(s) | 441, 507, 1207 |
| DraI | TTTAAA | 2 cut(s) | 205, 978 |
| Ecl136II | GAGCTC | 1 cut(s) | 1136 |
| Eco24I | GRGCYC | 1 cut(s) | 1138 |
| Eco31I | GGTCTC | 1 cut(s) | 1278 |
| Eco32I | GATATC | 1 cut(s) | 725 |
| Eco47I | GGWCC | 1 cut(s) | 1046 |
| Eco53kI | GAGCTC | 1 cut(s) | 1136 |
| Eco57I | CTGAAG | 4 cut(s) | 488, 881, 944, 1010 |
| Eco81I | CCTNAGG | 1 cut(s) | 134 |
| Eco88I | CYCGRG | 2 cut(s) | 539, 569 |
| Eco91I | GGTNACC | 1 cut(s) | 772 |
| EcoICRI | GAGCTC | 1 cut(s) | 1136 |
| EcoO65I | GGTNACC | 1 cut(s) | 772 |
| EcoRI | GAATTC | 1 cut(s) | 281 |
| EcoRII | CCWGG | 1 cut(s) | 669 |
| EcoRV | GATATC | 1 cut(s) | 725 |
| EcoT22I | ATGCAT | 1 cut(s) | 1058 |
| EcoT38I | GRGCYC | 1 cut(s) | 1138 |
| Esp3I | CGTCTC | 1 cut(s) | 684 |
| FaqI | GGGAC | 2 cut(s) | 129, 475 |
| FblI | GTMKAC | 1 cut(s) | 122 |
| Fnu4HI | GCNGC | 6 cut(s) | 62, 866, 882, 948, 1031, 1083 |
| FokI | GGATG | 1 cut(s) | 882 |
| FriOI | GRGCYC | 1 cut(s) | 1138 |
| Fsp4HI | GCNGC | 6 cut(s) | 62, 866, 882, 948, 1031, 1083 |
| FspBI | CTAG | 2 cut(s) | 96, 941 |
| GluI | GCNGC | 6 cut(s) | 62, 866, 882, 948, 1031, 1083 |
| GsuI | CTGGAG | 2 cut(s) | 549, 1211 |
| HaeIII | GGCC | 2 cut(s) | 884, 1249 |
| HincII | GTYRAC | 1 cut(s) | 151 |
| HindII | GTYRAC | 1 cut(s) | 151 |
| HinfI | GANTC | 5 cut(s) | 241, 337, 587, 856, 1014 |
| HphI | GGTGA | 4 cut(s) | 250, 661, 814, 1094 |
| Hpy166II | GTNNAC | 4 cut(s) | 7, 123, 151, 697 |
| Hpy188I | TCNGA | 6 cut(s) | 507, 512, 689, 769, 861, 924 |
| Hpy188III | TCNNGA | 5 cut(s) | 286, 389, 539, 571, 988 |
| Hpy8I | GTNNAC | 4 cut(s) | 7, 123, 151, 697 |
| HpyAV | CCTTC | 2 cut(s) | 985, 1329 |
| HpyCH4III | ACNGT | 4 cut(s) | 120, 359, 778, 1327 |
| HpyCH4IV | ACGT | 2 cut(s) | 693, 753 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 874 |
| HpyF3I | CTNAG | 4 cut(s) | 134, 768, 783, 1293 |
| HpySE526I | ACGT | 2 cut(s) | 693, 753 |
| Kzo9I | GATC | 3 cut(s) | 441, 507, 1207 |
| Lsp1109I | GCAGC | 4 cut(s) | 852, 959, 1042, 1094 |
| LweI | GCATC | 3 cut(s) | 133, 904, 1004 |
| MaeI | CTAG | 2 cut(s) | 96, 941 |
| MaeII | ACGT | 2 cut(s) | 693, 753 |
| MaeIII | GTNAC | 3 cut(s) | 523, 749, 772 |
| MalI | GATC | 3 cut(s) | 443, 509, 1209 |
| MboI | GATC | 3 cut(s) | 441, 507, 1207 |
| MboII | GAAGA | 4 cut(s) | 290, 422, 494, 811 |
| MfeI | CAATTG | 3 cut(s) | 159, 232, 630 |
| MflI | RGATCY | 2 cut(s) | 441, 507 |
| MhlI | GDGCHC | 2 cut(s) | 967, 1138 |
| MlyI | GAGTC | 1 cut(s) | 1008 |
| MmeI | TCCRAC | 2 cut(s) | 535, 870 |
| MnlI | CCTC | 8 cut(s) | 257, 503, 567, 763, 918, 1115, 1242, 1271 |
| Mph1103I | ATGCAT | 1 cut(s) | 1058 |
| MroXI | GAANNNNTTC | 1 cut(s) | 336 |
| MseI | TTAA | 6 cut(s) | 204, 327, 606, 611, 977, 1169 |
| MslI | CAYNNNNRTG | 1 cut(s) | 519 |
| MspA1I | CMGCKG | 3 cut(s) | 837, 1033, 1085 |
| MspR9I | CCNGG | 1 cut(s) | 671 |
| MunI | CAATTG | 3 cut(s) | 159, 232, 630 |
| MvaI | CCWGG | 1 cut(s) | 671 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 874 |
| NdeII | GATC | 3 cut(s) | 441, 507, 1207 |
| NlaIV | GGNNCC | 1 cut(s) | 1047 |
| NmuCI | GTSAC | 1 cut(s) | 523 |
| NsiI | ATGCAT | 1 cut(s) | 1058 |
| NspI | RCATGY | 4 cut(s) | 61, 518, 1056, 1160 |
| PaeI | GCATGC | 2 cut(s) | 61, 1056 |
| PaeR7I | CTCGAG | 2 cut(s) | 539, 569 |
| PciI | ACATGT | 2 cut(s) | 514, 1156 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 632, 699 |
| PdmI | GAANNNNTTC | 1 cut(s) | 336 |
| PfeI | GAWTC | 4 cut(s) | 241, 337, 587, 856 |
| PflFI | GACNNNGTC | 1 cut(s) | 11 |
| PkrI | GCNGC | 6 cut(s) | 63, 867, 883, 949, 1032, 1084 |
| PleI | GAGTC | 1 cut(s) | 1008 |
| PpsI | GAGTC | 1 cut(s) | 1008 |
| PscI | ACATGT | 2 cut(s) | 514, 1156 |
| PshBI | ATTAAT | 1 cut(s) | 327 |
| Psp124BI | GAGCTC | 1 cut(s) | 1138 |
| Psp6I | CCWGG | 1 cut(s) | 669 |
| PspEI | GGTNACC | 1 cut(s) | 772 |
| PspGI | CCWGG | 1 cut(s) | 669 |
| PspN4I | GGNNCC | 1 cut(s) | 1047 |
| PspPI | GGNCC | 1 cut(s) | 1046 |
| PsuI | RGATCY | 2 cut(s) | 441, 507 |
| PsyI | GACNNNGTC | 1 cut(s) | 11 |
| PvuII | CAGCTG | 3 cut(s) | 837, 1033, 1085 |
| RsaI | GTAC | 2 cut(s) | 756, 1215 |
| RsaNI | GTAC | 2 cut(s) | 755, 1214 |
| RseI | CAYNNNNRTG | 1 cut(s) | 519 |
| SacI | GAGCTC | 1 cut(s) | 1138 |
| SaqAI | TTAA | 6 cut(s) | 204, 327, 606, 611, 977, 1169 |
| SatI | GCNGC | 6 cut(s) | 62, 866, 882, 948, 1031, 1083 |
| Sau3AI | GATC | 3 cut(s) | 441, 507, 1207 |
| Sau96I | GGNCC | 1 cut(s) | 1046 |
| SchI | GAGTC | 1 cut(s) | 1008 |
| ScrFI | CCNGG | 1 cut(s) | 671 |
| SduI | GDGCHC | 2 cut(s) | 967, 1138 |
| SfaNI | GCATC | 3 cut(s) | 133, 904, 1004 |
| SfcI | CTRYAG | 2 cut(s) | 1326, 1343 |
| Sfr274I | CTCGAG | 2 cut(s) | 539, 569 |
| SinI | GGWCC | 1 cut(s) | 1046 |
| SlaI | CTCGAG | 2 cut(s) | 539, 569 |
| SmiI | ATTTAAAT | 1 cut(s) | 978 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 519 |
| SmlI | CTYRAG | 3 cut(s) | 539, 569, 1106 |
| SmoI | CTYRAG | 3 cut(s) | 539, 569, 1106 |
| SpeI | ACTAGT | 1 cut(s) | 940 |
| SphI | GCATGC | 2 cut(s) | 61, 1056 |
| SsiI | CCGC | 2 cut(s) | 62, 881 |
| SspI | AATATT | 1 cut(s) | 478 |
| SspMI | CTAG | 2 cut(s) | 96, 941 |
| SstI | GAGCTC | 1 cut(s) | 1138 |
| StyD4I | CCNGG | 1 cut(s) | 669 |
| SwaI | ATTTAAAT | 1 cut(s) | 978 |
| TaaI | ACNGT | 4 cut(s) | 120, 359, 778, 1327 |
| TaiI | ACGT | 2 cut(s) | 696, 756 |
| TaqI | TCGA | 4 cut(s) | 540, 570, 1132, 1263 |
| TauI | GCSGC | 2 cut(s) | 64, 884 |
| TfiI | GAWTC | 4 cut(s) | 241, 337, 587, 856 |
| Tru1I | TTAA | 6 cut(s) | 204, 327, 606, 611, 977, 1169 |
| Tru9I | TTAA | 6 cut(s) | 204, 327, 606, 611, 977, 1169 |
| TscAI | CASTG | 5 cut(s) | 22, 91, 364, 588, 1194 |
| TseFI | GTSAC | 1 cut(s) | 523 |
| TseI | GCWGC | 4 cut(s) | 865, 947, 1030, 1082 |
| Tsp45I | GTSAC | 1 cut(s) | 523 |
| TspDTI | ATGAA | 3 cut(s) | 345, 696, 959 |
| TspGWI | ACGGA | 1 cut(s) | 1231 |
| TspRI | CASTG | 5 cut(s) | 22, 91, 364, 588, 1194 |
| Tth111I | GACNNNGTC | 1 cut(s) | 11 |
| VpaK11BI | GGWCC | 1 cut(s) | 1046 |
| VspI | ATTAAT | 1 cut(s) | 327 |
| XapI | RAATTY | 2 cut(s) | 200, 281 |
| XceI | RCATGY | 4 cut(s) | 61, 518, 1056, 1160 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 598 |
| XhoI | CTCGAG | 2 cut(s) | 539, 569 |
| XmiI | GTMKAC | 1 cut(s) | 122 |
| XmnI | GAANNNNTTC | 1 cut(s) | 336 |
| XspI | CTAG | 2 cut(s) | 96, 941 |
| Zsp2I | ATGCAT | 1 cut(s) | 1058 |