RchiOBHm_Chr2g0105211

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000095
Physical Location & Seq
Forward (+)
16552139 .. 16553872
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47943

Sequence Viewer

Length: 951 bp
ATGATGGACAGAATCAGTGGATTGCCGGATGAAATTCTTGTGAGTATACTGTCTCTGTTGCCCCTGAAGGAAGCGCAAGCTACTAGTATCCTTTCTAGGAGGTGGCAGTATGTGTGGGCGTATTGTACTACTCTCAACTTTGATGATGAGAAGAATTTAGTCAGATTGCGCCTGTCCGACAGAGAAGCACTCGAATTGGAAATGTACCTACACGTCAATTGGGTGGATAGTTTGTTGAAACAGCATAGAACCTTAAACATTGAGCGATTCAGGGTTTATTTTAAGCTTGATTCTAGAAGTTGTATTGATAAATGGATTCAATTTGCAATGAGGAAAGGAGTTCATATGCTTGAGTTGGACTTTTCTGAATACATTGGTTTTAAGTGCCTCAAAGTTCTTGATTTGAAAACTGTTGATGTGGACCAAGAAGTTCTTGAGTACTTTTTGTCTTACTCTCGAGGTCTTGAACGACTGTCCGTGTCTGATTCTTCAACACTGGTTAATCTAAAAGTAACCGGTCATTCTATTGCATTGAAGTACTTGGTGATAAAACGATGTAAAAATATCAAAACCATTGAGATCTGTGATTCAAACCTAGTTTCATTTGCTTATGATGGACCTGTGAGAAACTTGGTTCTTAGTAATCTACCACTTCTTGTTGAGGTTTCCCTTTTTGGATGGTTGGCACCCGATTTCTTAGAGATTGCCTTCTTACAACTGTCCTGCTGTCTTTCTCAACTGGAGATTCTCAAACTCAGCTATGCGTGGAGCAAAATGGACCCTGTGATTCCTTCATTACCTGATCTTAAGCGTTTGGAATTAGTTATTGATGAAGATGAAAACTTTGCTCTTCTTCATCTCGCATCTTTCATTAAGGAATCTCCTTGCATGCATACACTAGTGTTGCGGATATGTATACTAAACAGTGTATTTTTTATGTGCACCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

316

Amino Acids

36.61

Weight (kDa)

5.76

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 5 - 40 8e-07 F-box domain
LRR_At1g61320_AtMIF1 PF23622 128 - 305 6.1e-20 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000095)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04260 FvH4_1g04270 FvH4_1g04271 FvH4_1g04280 FvH4_1g15681 FvH4_1g15870 FvH4_1g15871 FvH4_1g15880 FvH4_1g15890 FvH4_1g15890 FvH4_1g15890 FvH4_2g27801 FvH4_2g38801 FvH4_3g15922 FvH4_6g01850 FvH4_6g01850 FvH4_6g01850 FvH4_6g01850 FvH4_6g01861 FvH4_6g01970 FvH4_6g01970 FvH4_6g04740 FvH4_6g05140 FvH4_6g05150 FvH4_6g05160 FvH4_6g05620 FvH4_6g43901 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150 FvH4_7g08150
malus_domestica MD02G1016200.v1.1 MD02G1024100.v1.1 MD02G1024500.v1.1 MD02G1269100.v1.1 MD05G1360100.v1.1 MD08G1218100.v1.1 MD10G1330300.v1.1 MD11G1200500.v1.1 MD12G1064700.v1.1 MD14G1064800.v1.1 MD15G1437600.v1.1
prunus_persica Prupe.1G269300_v2.0.a1 Prupe.1G269300_v2.0.a1 Prupe.1G404400_v2.0.a1 Prupe.1G404400_v2.0.a1 Prupe.1G404600_v2.0.a1 Prupe.3G194200_v2.0.a1 Prupe.4G126500_v2.0.a1 Prupe.4G126500_v2.0.a1 Prupe.4G126500_v2.0.a1 Prupe.5G083600_v2.0.a1 Prupe.5G181600_v2.0.a1 Prupe.6G354300_v2.0.a1 Prupe.6G354300_v2.0.a1 Prupe.6G354500_v2.0.a1 Prupe.6G354600_v2.0.a1 Prupe.7G069200_v2.0.a1 Prupe.7G069300_v2.0.a1 Prupe.7G069300_v2.0.a1 Prupe.7G069300_v2.0.a1 Prupe.7G247200_v2.0.a1 Prupe.8G038200_v2.0.a1 Prupe.8G159200_v2.0.a1 Prupe.8G168400_v2.0.a1 Prupe.8G213700_v2.0.a1 Prupe.I001500_v2.0.a1
pyrus_communis pycom01g10450 pycom02g01440 pycom02g02050 pycom02g02060 pycom05g32510 pycom10g27970 pycom12g05660 pycom14g05250 pycom15g14470
rosa_chinensis RchiOBHm_Chr1g0330451 RchiOBHm_Chr1g0330541 RchiOBHm_Chr1g0360451 RchiOBHm_Chr2g0105201 RchiOBHm_Chr2g0105211 RchiOBHm_Chr2g0105241 RchiOBHm_Chr2g0105251 RchiOBHm_Chr2g0105261 RchiOBHm_Chr2g0160681 RchiOBHm_Chr3g0448841 RchiOBHm_Chr3g0448851 RchiOBHm_Chr3g0449531 RchiOBHm_Chr3g0449541 RchiOBHm_Chr3g0449551 RchiOBHm_Chr3g0449571 RchiOBHm_Chr3g0449581 RchiOBHm_Chr3g0449591 RchiOBHm_Chr3g0449601 RchiOBHm_Chr3g0449821 RchiOBHm_Chr3g0471621 RchiOBHm_Chr3g0480841 RchiOBHm_Chr5g0026831 RchiOBHm_Chr5g0026841 RchiOBHm_Chr6g0298681 RchiOBHm_Chr6g0298691 RchiOBHm_Chr6g0302471 RchiOBHm_Chr7g0184471 RchiOBHm_Chr7g0184481 RchiOBHm_Chr7g0197551
rosa_laevigata RLG00000003925 RLG00000004972 RLG00000016059 RLG00000017409 RLG00000023448 RLG00000023621 RLG00000025792 RLG00000025793 RLG00000025816 RLG00000025871 RLG00000027807 RLG00000029809 RLG00000032963
rosa_multiflora Rmu_co8092536.1_g000001 Rmu_co8349415.1_g000001 Rmu_co8375699.1_g000001 Rmu_co8381287.1_g000001 Rmu_co8442591.1_g000001 Rmu_co8442591.1_g000002 Rmu_co8446009.1_g000001 Rmu_co8468183.1_g000001 Rmu_sc0000430.1_g000001 Rmu_sc0000430.1_g000004 Rmu_sc0000430.1_g000012 Rmu_sc0000610.1_g000007 Rmu_sc0001122.1_g000011 Rmu_sc0001170.1_g000015 Rmu_sc0001407.1_g000011 Rmu_sc0001557.1_g000012 Rmu_sc0001557.1_g000013 Rmu_sc0002200.1_g000011 Rmu_sc0004831.1_g000010 Rmu_sc0004838.1_g000013 Rmu_sc0006734.1_g000010 Rmu_sc0007290.1_g000003 Rmu_sc0011021.1_g000011 Rmu_sc0012217.1_g000006 Rmu_sc0012984.1_g000017 Rmu_sc0013983.1_g000003 Rmu_sc0018347.1_g000001 Rmu_sc0021523.1_g000001 Rmu_sc0026421.1_g000001 Rmu_sc0026421.1_g000002 Rmu_sc0027913.1_g000002 Rmu_sc0037242.1_g000001 Rmu_ssc0000031.1_g000002 Rmu_ssc0000031.1_g000003 Rmu_ssc0000172.1_g000019 Rmu_ssc0000337.1_g000013 Rmu_ssc0000368.1_g000069
rosa_roxburghii Rroxscaffold_1G00013770 Rroxscaffold_1G00017620 Rroxscaffold_1G00052670 Rroxscaffold_1G00052680 Rroxscaffold_1G00052690 Rroxscaffold_2G00089490 Rroxscaffold_2G00089510 Rroxscaffold_2G00138340 Rroxscaffold_2G00138350 Rroxscaffold_2G00138380 Rroxscaffold_2G00151560 Rroxscaffold_2G00151570 Rroxscaffold_3G00220490 Rroxscaffold_3G00258350 Rroxscaffold_4G00296100 Rroxscaffold_6G00400970 Rroxscaffold_6G00403030 Rroxscaffold_6G00427050 Rroxscaffold_6G00427490 Rroxscaffold_6G00427500 Rroxscaffold_6G00427640 Rroxscaffold_6G00427660 Rroxscaffold_7G00165730 Rroxscaffold_7G00165740
rosa_rugosa Rorug01G0194900 Rorug01G0277200 Rorug01G0277300 Rorug01G0277400 Rorug01G0277500 Rorug01G0277600 Rorug01G0277700 Rorug01G0277800 Rorug02G0003600 Rorug02G0126800 Rorug02G0126900 Rorug02G0484800 Rorug02G0484900 Rorug02G0623600 Rorug03G0186800 Rorug06G0314400 Rorug07G0041200 Rorug07G0041300 Rorug07G0041300 Rorug07G0041400
rosa_samantha Rh1AG107100 Rh1AG174800 Rh1AG212900 Rh1AG291000 Rh1BG085200 Rh1DG112700 Rh2AG048800 Rh2AG177600 Rh2AG549900 Rh2BG047600 Rh2BG185700 Rh2BG185800 Rh2BG186000 Rh2BG186100 Rh2BG564400 Rh2CG049700 Rh2CG182100 Rh2CG182300 Rh3BG014900 Rh3BG020400 Rh3BG020500 Rh3BG020600 Rh3BG020700 Rh3BG020800 Rh3BG020900 Rh3BG022500 Rh3BG195200 Rh3BG271900 Rh3CG019100 Rh3CG019200 Rh3CG019300 Rh3CG019400 Rh3CG021200 Rh3CG267700 Rh3DG020400 Rh3DG020500 Rh3DG020600 Rh3DG020700 Rh3DG022200 Rh3DG264000 Rh5BG185300 Rh5BG185500 Rh5BG186000 Rh5BG429000 Rh6AG427600 Rh6CG439700 Rh6DG426700 Rh7BG067600 Rh7CG067900 Rh7CG068000 Rh7CG174300 Rh7DG167300
rosa_wichuraiana Rw0G006170 Rw1G008880 Rw1G014570 Rw1G025770 Rw2G013940 Rw2G013960 Rw2G045560 Rw3G001610 Rw3G001620 Rw3G001630 Rw3G001650 Rw3G001790 Rw3G021340 Rw4G018910 Rw5G017150 Rw5G017160 Rw7G014440 Rw7G040330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 685
AccI GTMKAC 2 cut(s) 46, 916
AciI CCGC 1 cut(s) 907
AcsI RAATTY 2 cut(s) 33, 154
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 4 cut(s) 127, 206, 440, 539
AflII CTTAAG 1 cut(s) 806
AflIII ACRYGT 1 cut(s) 211
AgeI ACCGGT 1 cut(s) 515
AgsI TTSAA 7 cut(s) 238, 320, 406, 467, 492, 535, 591
AhlI ACTAGT 2 cut(s) 83, 898
AjiI CACGTC 1 cut(s) 214
AluBI AGCT 3 cut(s) 80, 286, 759
AluI AGCT 3 cut(s) 80, 286, 759
Alw21I GWGCWC 1 cut(s) 944
Alw26I GTCTC 1 cut(s) 57
Alw44I GTGCAC 1 cut(s) 940
Ama87I CYCGRG 1 cut(s) 456
ApaLI GTGCAC 1 cut(s) 940
ApoI RAATTY 2 cut(s) 33, 154
AsiGI ACCGGT 1 cut(s) 515
AspLEI GCGC 2 cut(s) 76, 171
AspS9I GGNCC 3 cut(s) 421, 617, 778
AsuHPI GGTGA 1 cut(s) 556
AvaI CYCGRG 1 cut(s) 456
AvaII GGWCC 3 cut(s) 421, 617, 778
BaeGI GKGCMC 1 cut(s) 944
BanI GGYRCC 1 cut(s) 685
Bbv12I GWGCWC 1 cut(s) 944
BccI CCATC 2 cut(s) 608, 672
BciVI GTATCC 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 57
BcuI ACTAGT 2 cut(s) 83, 898
BfaI CTAG 5 cut(s) 84, 96, 294, 596, 899
BfrI CTTAAG 1 cut(s) 806
BfuI GTATCC 1 cut(s) 98
BglII AGATCT 1 cut(s) 579
BmcAI AGTACT 2 cut(s) 440, 539
Bme18I GGWCC 3 cut(s) 421, 617, 778
BmeT110I CYCGRG 1 cut(s) 456
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 3 cut(s) 421, 617, 778
BmiI GGNNCC 2 cut(s) 687, 780
BmsI GCATC 1 cut(s) 872
BplI GAGNNNNNCTC 2 cut(s) 174, 206
BpmI CTGGAG 1 cut(s) 761
BpuEI CTTGAG 2 cut(s) 371, 455
BsaWI WCCGGW 1 cut(s) 515
Bse118I RCCGGY 1 cut(s) 515
Bse1I ACTGG 2 cut(s) 501, 744
Bse3DI GCAATG 1 cut(s) 333
BseGI GGATG 2 cut(s) 34, 683
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 1 cut(s) 769
BseNI ACTGG 2 cut(s) 501, 744
BseSI GKGCMC 1 cut(s) 944
BshNI GGYRCC 1 cut(s) 685
BshTI ACCGGT 1 cut(s) 515
BsiHKAI GWGCWC 1 cut(s) 944
BsiHKCI CYCGRG 1 cut(s) 456
BsiSI CCGG 2 cut(s) 26, 516
BsmAI GTCTC 1 cut(s) 57
BsoBI CYCGRG 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 944
Bsp143I GATC 2 cut(s) 579, 802
BspACI CCGC 1 cut(s) 907
BspCNI CTCAG 1 cut(s) 768
BspLI GGNNCC 2 cut(s) 687, 780
BspQI GCTCTTC 1 cut(s) 855
BspT107I GGYRCC 1 cut(s) 685
BspTI CTTAAG 1 cut(s) 806
BsrDI GCAATG 1 cut(s) 333
BsrFI RCCGGY 1 cut(s) 515
BsrI ACTGG 2 cut(s) 501, 744
BssAI RCCGGY 1 cut(s) 515
BssMI GATC 2 cut(s) 579, 802
BssNAI GTATAC 2 cut(s) 47, 917
Bst1107I GTATAC 2 cut(s) 47, 917
Bst4CI ACNGT 5 cut(s) 51, 412, 474, 720, 926
Bst6I CTCTTC 1 cut(s) 855
BstAFI CTTAAG 1 cut(s) 806
BstC8I GCNNGC 2 cut(s) 78, 890
BstDEI CTNAG 3 cut(s) 638, 697, 755
BstF5I GGATG 2 cut(s) 34, 683
BstHHI GCGC 2 cut(s) 76, 171
BstKTI GATC 2 cut(s) 582, 805
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 2 cut(s) 579, 802
BstNSI RCATGY 1 cut(s) 892
BstSLI GKGCMC 1 cut(s) 944
BstX2I RGATCY 1 cut(s) 579
BstYI RGATCY 1 cut(s) 579
BstZ17I GTATAC 2 cut(s) 47, 917
BsuI GTATCC 1 cut(s) 98
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 2 cut(s) 34, 683
BtsIMutI CAGTG 3 cut(s) 22, 494, 931
Cac8I GCNNGC 2 cut(s) 78, 890
CfoI GCGC 2 cut(s) 76, 171
Cfr10I RCCGGY 1 cut(s) 515
Cfr13I GGNCC 3 cut(s) 421, 617, 778
Csp6I GTAC 4 cut(s) 126, 205, 439, 538
CspAI ACCGGT 1 cut(s) 515
CviAII CATG 1 cut(s) 889
CviJI RGCY 3 cut(s) 80, 286, 759
CviKI_1 RGCY 3 cut(s) 80, 286, 759
CviQI GTAC 4 cut(s) 126, 205, 439, 538
DdeI CTNAG 3 cut(s) 638, 697, 755
DpnI GATC 2 cut(s) 581, 804
DpnII GATC 2 cut(s) 579, 802
Eam1104I CTCTTC 1 cut(s) 855
EarI CTCTTC 1 cut(s) 855
Eco47I GGWCC 3 cut(s) 421, 617, 778
Eco57I CTGAAG 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 456
EcoT22I ATGCAT 1 cut(s) 894
FaeI CATG 1 cut(s) 892
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FatI CATG 1 cut(s) 888
FauNDI CATATG 1 cut(s) 345
FblI GTMKAC 2 cut(s) 46, 916
FokI GGATG 2 cut(s) 41, 690
FspBI CTAG 5 cut(s) 84, 96, 294, 596, 899
GlaI GCGC 2 cut(s) 75, 170
GsuI CTGGAG 1 cut(s) 761
HapII CCGG 2 cut(s) 26, 516
HhaI GCGC 2 cut(s) 76, 171
Hin1II CATG 1 cut(s) 892
Hin6I GCGC 2 cut(s) 74, 169
HinP1I GCGC 2 cut(s) 74, 169
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 9 cut(s) 12, 267, 290, 316, 485, 587, 745, 787, 878
HpaII CCGG 2 cut(s) 26, 516
HphI GGTGA 1 cut(s) 556
Hpy166II GTNNAC 4 cut(s) 47, 421, 917, 942
Hpy188I TCNGA 4 cut(s) 164, 178, 367, 484
Hpy188III TCNNGA 6 cut(s) 294, 398, 434, 456, 464, 948
Hpy8I GTNNAC 4 cut(s) 47, 421, 917, 942
HpyAV CCTTC 3 cut(s) 61, 718, 801
HpyCH4III ACNGT 5 cut(s) 51, 412, 474, 720, 926
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 5 cut(s) 326, 530, 888, 892, 942
HpyF3I CTNAG 3 cut(s) 638, 697, 755
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 1 cut(s) 892
HspAI GCGC 2 cut(s) 74, 169
Kzo9I GATC 2 cut(s) 579, 802
LguI GCTCTTC 1 cut(s) 855
LmnI GCTCC 1 cut(s) 768
LweI GCATC 1 cut(s) 872
MaeI CTAG 5 cut(s) 84, 96, 294, 596, 899
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 1 cut(s) 511
MalI GATC 2 cut(s) 581, 804
MboI GATC 2 cut(s) 579, 802
MboII GAAGA 5 cut(s) 163, 480, 842, 845, 845
MfeI CAATTG 1 cut(s) 217
MflI RGATCY 1 cut(s) 579
MhlI GDGCHC 1 cut(s) 944
MluCI AATT 6 cut(s) 33, 154, 194, 217, 320, 818
MmeI TCCRAC 2 cut(s) 201, 336
MnlI CCTC 5 cut(s) 93, 324, 398, 452, 655
Mph1103I ATGCAT 1 cut(s) 894
MseI TTAA 6 cut(s) 254, 282, 381, 501, 807, 873
MspCI CTTAAG 1 cut(s) 806
MspI CCGG 2 cut(s) 26, 516
MunI CAATTG 1 cut(s) 217
NdeI CATATG 1 cut(s) 345
NdeII GATC 2 cut(s) 579, 802
NlaIII CATG 1 cut(s) 892
NlaIV GGNNCC 2 cut(s) 687, 780
NsiI ATGCAT 1 cut(s) 894
NspI RCATGY 1 cut(s) 892
PaeI GCATGC 1 cut(s) 892
PaeR7I CTCGAG 1 cut(s) 456
PciSI GCTCTTC 1 cut(s) 855
PfeI GAWTC 9 cut(s) 12, 267, 290, 316, 485, 587, 745, 787, 878
PinAI ACCGGT 1 cut(s) 515
PspN4I GGNNCC 2 cut(s) 687, 780
PspPI GGNCC 3 cut(s) 421, 617, 778
PsuI RGATCY 1 cut(s) 579
RsaI GTAC 4 cut(s) 127, 206, 440, 539
RsaNI GTAC 4 cut(s) 126, 205, 439, 538
SapI GCTCTTC 1 cut(s) 855
SaqAI TTAA 6 cut(s) 254, 282, 381, 501, 807, 873
Sau3AI GATC 2 cut(s) 579, 802
Sau96I GGNCC 3 cut(s) 421, 617, 778
ScaI AGTACT 2 cut(s) 440, 539
SduI GDGCHC 1 cut(s) 944
SfaNI GCATC 1 cut(s) 872
Sfr274I CTCGAG 1 cut(s) 456
SinI GGWCC 3 cut(s) 421, 617, 778
SlaI CTCGAG 1 cut(s) 456
SmlI CTYRAG 4 cut(s) 350, 434, 456, 806
SmoI CTYRAG 4 cut(s) 350, 434, 456, 806
SpeI ACTAGT 2 cut(s) 83, 898
SphI GCATGC 1 cut(s) 892
Sse9I AATT 6 cut(s) 33, 154, 194, 217, 320, 818
SsiI CCGC 1 cut(s) 907
SspMI CTAG 5 cut(s) 84, 96, 294, 596, 899
TaaI ACNGT 5 cut(s) 51, 412, 474, 720, 926
TaiI ACGT 1 cut(s) 216
TaqI TCGA 2 cut(s) 192, 457
TasI AATT 6 cut(s) 33, 154, 194, 217, 320, 818
TatI WGTACW 3 cut(s) 125, 438, 537
TfiI GAWTC 9 cut(s) 12, 267, 290, 316, 485, 587, 745, 787, 878
Tru1I TTAA 6 cut(s) 254, 282, 381, 501, 807, 873
Tru9I TTAA 6 cut(s) 254, 282, 381, 501, 807, 873
TscAI CASTG 3 cut(s) 22, 501, 931
TspDTI ATGAA 8 cut(s) 45, 332, 591, 783, 845, 846, 852, 859
TspGWI ACGGA 1 cut(s) 466
TspRI CASTG 3 cut(s) 22, 501, 931
Vha464I CTTAAG 1 cut(s) 806
VneI GTGCAC 1 cut(s) 940
VpaK11BI GGWCC 3 cut(s) 421, 617, 778
XapI RAATTY 2 cut(s) 33, 154
XbaI TCTAGA 1 cut(s) 293
XceI RCATGY 1 cut(s) 892
XhoI CTCGAG 1 cut(s) 456
XmiI GTMKAC 2 cut(s) 46, 916
XspI CTAG 5 cut(s) 84, 96, 294, 596, 899
ZrmI AGTACT 2 cut(s) 440, 539
Zsp2I ATGCAT 1 cut(s) 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.