FvH4_4g00640

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000079
Physical Location & Seq
Forward (+)
553218 .. 554524
1307 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00640.t1

Sequence Viewer

Length: 954 bp
ATGAAAAACAGTGGAGATCTCAAACTTGCACATGAAGATTCAATATCAATACTCCAAGCTGTGGAAGTTGACAGGATTATTGCACTACCGGATGAGATTCTCTGTCATATACTCTCTTTCCTTATCCCGTTATGTTCTGTCCGGAGCACCGTTTTGTCTAAGAGATGGAACAAGTTGTGGACTTGTTGTATTACCAACCTTGATTTCGACATAAAAGATTTCAGTTCATCATATGAGAGTAACTATCACATTTTCGTCAGGTTCGTTGATCAGGTGTTGGCCTTTCGCGACTCTTCATTAGACATCACAAGATTTCGAGTTGCCTGGGAGGATTGTGAAGATTTCTCTATTATTGATCGATGGATACAGGTCGCCGTCAACGCTAATGTTGTTGATCTTGATGTTTCTAGCTATGTGTATCCTTTCGAATTGCCTTCATGTGTTTATACCTGCAAATCACTGGTGTCTTTGAAGGTGAAGTCAGATTATCTTACATTTTATTCTCTTCCTAAGACAGGTAGTTGTTTCCCAAATCTCAAGTTCCTTCATTATATTTCTGGTTATGATTTCGACTGTGCCTCTGGGGCAAATCTTTTCCGCAGCTGCCCTATACTCGAAGAGTTGACTATAGAAGCACATCATAAGAAACGTGTTTTGAATTTCGACATCTCTGCACCTGAATTGAGGACACTAACCACAATCATGAAGAAGATGAATAAAAGAGAACATTATGAAGAAGATGAATTTGAGGAAGATTATGAAGAAGATTATAGGATAGACCCCTGCAATTTCTTTATCGATGCACCTAAACTTGAAAATCTTTGTGTTAAGGGAGAGGTTATGTCAAAGTATAATTGGAAGAACACAAGCTCTTTGGTTCAAGCCAGAGTTCAACTCCAAGTGTTCAATGAAGCTTCCGCTTGTTGTCTACAACCAGAAGAGCAAAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

318

Amino Acids

36.75

Weight (kDa)

4.94

Isoelectric Point (pI)

56.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 118 - 241 1e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000079)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32020
fragaria_vesca FvH4_2g37070 FvH4_3g17062 FvH4_3g17580 FvH4_3g17580 FvH4_3g17580 FvH4_3g17581 FvH4_3g18162 FvH4_3g20210 FvH4_3g32940 FvH4_3g33370 FvH4_3g33390 FvH4_3g33400 FvH4_3g33400 FvH4_3g33400 FvH4_3g33401 FvH4_3g33430 FvH4_4g00640 FvH4_5g02210 FvH4_5g02220 FvH4_5g02220 FvH4_5g02221 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02280 FvH4_5g02290 FvH4_5g02290 FvH4_5g08740 FvH4_5g24470 FvH4_6g07500 FvH4_6g07500 FvH4_6g07500 FvH4_6g07500 FvH4_6g08520 FvH4_6g10000 FvH4_7g28000 FvH4_7g28011 FvH4_7g28030 FvH4_7g28040 FvH4_7g28042
malus_domestica MD03G1112000.v1.1 MD03G1112100.v1.1 MD03G1112200.v1.1 MD03G1253000.v1.1 MD03G1253100.v1.1 MD03G1253400.v1.1 MD03G1255400.v1.1 MD03G1276300.v1.1 MD03G1276400.v1.1 MD05G1193700.v1.1 MD11G1265000.v1.1 MD11G1265100.v1.1 MD11G1265300.v1.1 MD11G1275400.v1.1 MD11G1275600.v1.1
prunus_persica Prupe.4G151700_v2.0.a1 Prupe.4G160800_v2.0.a1 Prupe.4G160900_v2.0.a1 Prupe.4G161000_v2.0.a1 Prupe.4G161100_v2.0.a1 Prupe.4G161200_v2.0.a1 Prupe.4G161300_v2.0.a1 Prupe.5G021800_v2.0.a1
pyrus_communis pycom03g06140 pycom03g19340 pycom03g20150 pycom03g20210 pycom05g18110 pycom11g23320 pycom11g24360 pycom11g24380
rosa_chinensis RchiOBHm_Chr1g0321391 RchiOBHm_Chr1g0363171 RchiOBHm_Chr1g0363181 RchiOBHm_Chr2g0115721 RchiOBHm_Chr3g0458571 RchiOBHm_Chr3g0460681 RchiOBHm_Chr4g0387591 RchiOBHm_Chr5g0028191 RchiOBHm_Chr5g0028201 RchiOBHm_Chr5g0028241 RchiOBHm_Chr5g0028261 RchiOBHm_Chr5g0028411 RchiOBHm_Chr5g0028431 RchiOBHm_Chr5g0029291 RchiOBHm_Chr5g0030601 RchiOBHm_Chr5g0030631 RchiOBHm_Chr5g0059941 RchiOBHm_Chr5g0059951 RchiOBHm_Chr5g0059961 RchiOBHm_Chr5g0060061 RchiOBHm_Chr5g0060761 RchiOBHm_Chr5g0060771 RchiOBHm_Chr6g0304711 RchiOBHm_Chr7g0192751 RchiOBHm_Chr7g0197861 RchiOBHm_Chr7g0197881 RchiOBHm_Chr7g0197891 RchiOBHm_Chr7g0197921
rosa_laevigata RLG00000003903 RLG00000003904 RLG00000004332 RLG00000010109 RLG00000010957 RLG00000018203 RLG00000024989 RLG00000025147 RLG00000027586 RLG00000030426 RLG00000033074 RLG00000033075 RLG00000033076 RLG00000033094 RLG00000033150 RLG00000033234 RLG00000035345 RLG00000035399
rosa_multiflora Rmu_co8271835.1_g000001 Rmu_co8353127.1_g000001 Rmu_co8434667.1_g000001 Rmu_co8434713.1_g000001 Rmu_co8441243.1_g000001 Rmu_co8501607.1_g000001 Rmu_sc0000471.1_g000010 Rmu_sc0000724.1_g000008 Rmu_sc0000727.1_g000033 Rmu_sc0000814.1_g000032 Rmu_sc0000960.1_g000003 Rmu_sc0001082.1_g000014 Rmu_sc0001082.1_g000016 Rmu_sc0001571.1_g000005 Rmu_sc0001571.1_g000007 Rmu_sc0001571.1_g000008 Rmu_sc0001571.1_g000020 Rmu_sc0002040.1_g000017 Rmu_sc0002925.1_g000003 Rmu_sc0003864.1_g000006 Rmu_sc0005482.1_g000001 Rmu_sc0005482.1_g000005 Rmu_sc0006771.1_g000006 Rmu_sc0007143.1_g000008 Rmu_sc0007248.1_g000006 Rmu_sc0011593.1_g000004 Rmu_sc0011593.1_g000006 Rmu_sc0011593.1_g000007 Rmu_sc0011593.1_g000012 Rmu_sc0016104.1_g000007 Rmu_sc0022819.1_g000001 Rmu_sc0025685.1_g000002 Rmu_sc0027256.1_g000002 Rmu_sc0034563.1_g000001 Rmu_sc0034642.1_g000001 Rmu_ssc0000197.1_g000059
rosa_roxburghii Rroxscaffold_1G00019950 Rroxscaffold_1G00020580 Rroxscaffold_1G00051250 Rroxscaffold_1G00051270 Rroxscaffold_1G00051470 Rroxscaffold_1G00051480 Rroxscaffold_2G00127930 Rroxscaffold_2G00128790 Rroxscaffold_3G00258110 Rroxscaffold_3G00258130 Rroxscaffold_6G00419310 Rroxscaffold_6G00420970 Rroxscaffold_6G00421360 Rroxscaffold_7G00163570
rosa_rugosa Rorug01G0032500 Rorug02G0199500.1 Rorug02G0199600.1 Rorug03G0024200 Rorug03G0029800 Rorug03G0029900 Rorug03G0045000 Rorug03G0045000 Rorug03G0310900 Rorug05G0104800 Rorug05G0104900 Rorug05G0105000 Rorug05G0105100 Rorug05G0105200 Rorug05G0105300 Rorug05G0107400 Rorug05G0107600 Rorug05G0112900 Rorug05G0118900 Rorug05G0327000 Rorug05G0327000 Rorug05G0327000 Rorug05G0328000 Rorug05G0333900 Rorug06G0333700 Rorug07G0004400 Rorug07G0044100 Rorug07G0044200 Rorug07G0044200 Rorug07G0044300 Rorug07G0044400
rosa_samantha Rh1AG314000 Rh1BG277500 Rh1CG293900 Rh1DG051800 Rh1DG308100 Rh1DG308200 Rh2AG256700 Rh2AG295900 Rh2BG267300 Rh2CG262300 Rh2DG265100 Rh2DG319900 Rh3AG084700 Rh3AG088100 Rh3AG102600 Rh3BG087500 Rh3CG088000 Rh3CG091300 Rh3CG108200 Rh3DG088400 Rh4AG017000 Rh4CG018100 Rh4DG012700 Rh5AG198100 Rh5AG198200 Rh5AG198300 Rh5AG198500 Rh5AG198600 Rh5AG198700 Rh5AG200400 Rh5AG205000 Rh5AG211100 Rh5AG244900 Rh5AG391100 Rh5AG391200 Rh5AG396500 Rh5BG197000 Rh5BG197100 Rh5BG197200 Rh5BG197300 Rh5BG198600 Rh5BG198800 Rh5BG403400 Rh5BG403500 Rh5CG216700 Rh5CG216900 Rh5CG217000 Rh5CG218500 Rh5CG225000 Rh5CG427400 Rh5CG427500 Rh5CG433300 Rh5DG198400 Rh5DG198500 Rh5DG198800 Rh5DG198900 Rh5DG199000 Rh5DG200700 Rh5DG201000 Rh5DG207200 Rh5DG215000 Rh5DG374300 Rh5DG419000 Rh5DG419100 Rh5DG420200 Rh5DG423900 Rh6BG456100 Rh6CG458700 Rh7AG129400 Rh7AG168100 Rh7AG457700 Rh7BG170900 Rh7CG177400 Rh7CG177500 Rh7DG131600 Rh7DG169800 Rh7DG169900 Rh7DG170000 Rh7DG170100
rosa_wichuraiana Rw1G027820 Rw2G020090 Rw3G007530 Rw3G008730 Rw4G001080 Rw5G018080 Rw5G018090 Rw5G018100 Rw5G018110 Rw5G018120 Rw5G018640 Rw5G019310 Rw5G036810 Rw5G036820 Rw5G036830 Rw5G037390 Rw6G038770 Rw7G011070 Rw7G014650 Rw7G014660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 458
AccB7I CCANNNNNTGG 1 cut(s) 61
AccI GTMKAC 1 cut(s) 928
AccII CGCG 1 cut(s) 288
AccIII TCCGGA 1 cut(s) 141
AciI CCGC 2 cut(s) 598, 918
AcsI RAATTY 2 cut(s) 658, 743
AfiI CCNNNNNNNGG 2 cut(s) 61, 515
AflIII ACRYGT 1 cut(s) 649
AgsI TTSAA 7 cut(s) 42, 472, 658, 815, 881, 893, 907
AjnI CCWGG 1 cut(s) 323
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AluBI AGCT 5 cut(s) 59, 411, 603, 870, 914
AluI AGCT 5 cut(s) 59, 411, 603, 870, 914
Alw21I GWGCWC 1 cut(s) 149
Aor13HI TCCGGA 1 cut(s) 141
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 2 cut(s) 600, 603
ApoI RAATTY 2 cut(s) 658, 743
AsuHPI GGTGA 1 cut(s) 487
AsuII TTCGAA 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 149
BbvI GCAGC 2 cut(s) 590, 612
BccI CCATC 2 cut(s) 159, 354
BceAI ACGGC 1 cut(s) 359
BcgI CGANNNNNNTGC 2 cut(s) 653, 687
BciT130I CCWGG 1 cut(s) 325
BciVI GTATCC 2 cut(s) 357, 429
BclI TGATCA 1 cut(s) 268
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 627
BfuAI ACCTGC 1 cut(s) 458
BfuI GTATCC 2 cut(s) 357, 429
BglI GCCNNNNNGGC 1 cut(s) 584
BglII AGATCT 1 cut(s) 16
BisI GCNGC 2 cut(s) 601, 604
BlsI GCNGC 2 cut(s) 602, 605
Bme1390I CCNGG 1 cut(s) 325
BmrFI CCNGG 1 cut(s) 325
BmsI GCATC 1 cut(s) 790
BplI GAGNNNNNCTC 2 cut(s) 879, 911
Bpu14I TTCGAA 1 cut(s) 426
BpuEI CTTGAG 1 cut(s) 521
Bsa29I ATCGAT 2 cut(s) 358, 798
BsaJI CCNNGG 1 cut(s) 324
BsaWI WCCGGW 2 cut(s) 88, 141
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 515
Bse1I ACTGG 1 cut(s) 465
BseAI TCCGGA 1 cut(s) 141
BseBI CCWGG 1 cut(s) 325
BseCI ATCGAT 2 cut(s) 358, 798
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 1 cut(s) 97
BseLI CCNNNNNNNGG 2 cut(s) 61, 515
BseNI ACTGG 1 cut(s) 465
BseXI GCAGC 2 cut(s) 590, 612
BsgI GTGCAG 1 cut(s) 657
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 1 cut(s) 281
BshVI ATCGAT 2 cut(s) 358, 798
BsiHKAI GWGCWC 1 cut(s) 149
BsiSI CCGG 2 cut(s) 89, 142
BslI CCNNNNNNNGG 2 cut(s) 61, 515
BsnI GGCC 1 cut(s) 281
Bsp119I TTCGAA 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 149
Bsp13I TCCGGA 1 cut(s) 141
Bsp143I GATC 4 cut(s) 16, 268, 355, 394
Bsp68I TCGCGA 1 cut(s) 288
BspACI CCGC 2 cut(s) 598, 918
BspANI GGCC 1 cut(s) 281
BspDI ATCGAT 2 cut(s) 358, 798
BspEI TCCGGA 1 cut(s) 141
BspFNI CGCG 1 cut(s) 288
BspHI TCATGA 1 cut(s) 702
BspMI ACCTGC 1 cut(s) 458
BspQI GCTCTTC 1 cut(s) 933
BspT104I TTCGAA 1 cut(s) 426
BsrI ACTGG 1 cut(s) 465
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 4 cut(s) 16, 268, 355, 394
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 3 cut(s) 11, 151, 575
Bst6I CTCTTC 4 cut(s) 298, 510, 612, 933
BstBI TTCGAA 1 cut(s) 426
BstDEI CTNAG 2 cut(s) 159, 510
BstENI CCTNNNNNAGG 1 cut(s) 513
BstF5I GGATG 1 cut(s) 97
BstFNI CGCG 1 cut(s) 288
BstKTI GATC 4 cut(s) 19, 271, 358, 397
BstMBI GATC 4 cut(s) 16, 268, 355, 394
BstMWI GCNNNNNNNGC 2 cut(s) 380, 584
BstNI CCWGG 1 cut(s) 325
BstSCI CCNGG 1 cut(s) 323
BstSFI CTRYAG 1 cut(s) 627
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 2 cut(s) 590, 612
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
Bsu15I ATCGAT 2 cut(s) 358, 798
BsuI GTATCC 2 cut(s) 357, 429
BsuRI GGCC 1 cut(s) 281
BsuTUI ATCGAT 2 cut(s) 358, 798
BtsCI GGATG 1 cut(s) 97
BtsIMutI CAGTG 2 cut(s) 16, 458
BtuMI TCGCGA 1 cut(s) 288
BveI ACCTGC 1 cut(s) 458
CciI TCATGA 1 cut(s) 702
ClaI ATCGAT 2 cut(s) 358, 798
CviAII CATG 3 cut(s) 32, 438, 703
CviJI RGCY 7 cut(s) 59, 281, 411, 603, 870, 884, 914
CviKI_1 RGCY 7 cut(s) 59, 281, 411, 603, 870, 884, 914
DdeI CTNAG 2 cut(s) 159, 510
DpnI GATC 4 cut(s) 18, 270, 357, 396
DpnII GATC 4 cut(s) 16, 268, 355, 394
Eam1104I CTCTTC 4 cut(s) 298, 510, 612, 933
EarI CTCTTC 4 cut(s) 298, 510, 612, 933
EcoNI CCTNNNNNAGG 1 cut(s) 513
EcoRII CCWGG 1 cut(s) 323
FaeI CATG 3 cut(s) 35, 441, 706
FatI CATG 3 cut(s) 31, 437, 702
FauNDI CATATG 1 cut(s) 232
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 1 cut(s) 928
Fnu4HI GCNGC 2 cut(s) 601, 604
FokI GGATG 1 cut(s) 104
Fsp4HI GCNGC 2 cut(s) 601, 604
FspBI CTAG 1 cut(s) 408
GluI GCNGC 2 cut(s) 601, 604
HaeIII GGCC 1 cut(s) 281
HapII CCGG 2 cut(s) 89, 142
Hin1II CATG 3 cut(s) 35, 441, 706
HincII GTYRAC 3 cut(s) 70, 379, 624
HindII GTYRAC 3 cut(s) 70, 379, 624
HindIII AAGCTT 1 cut(s) 912
HinfI GANTC 3 cut(s) 38, 97, 290
HpaII CCGG 2 cut(s) 89, 142
HphI GGTGA 1 cut(s) 487
Hpy166II GTNNAC 5 cut(s) 70, 180, 379, 624, 929
Hpy188I TCNGA 1 cut(s) 484
Hpy188III TCNNGA 4 cut(s) 142, 287, 398, 703
Hpy8I GTNNAC 5 cut(s) 70, 180, 379, 624, 929
HpyAV CCTTC 3 cut(s) 444, 466, 554
HpyCH4III ACNGT 3 cut(s) 11, 151, 575
HpyCH4IV ACGT 1 cut(s) 649
HpyCH4V TGCA 6 cut(s) 29, 83, 453, 674, 786, 803
HpyF10VI GCNNNNNNNGC 2 cut(s) 380, 584
HpyF3I CTNAG 2 cut(s) 159, 510
HpySE526I ACGT 1 cut(s) 649
Hsp92II CATG 3 cut(s) 35, 441, 706
Kpn2I TCCGGA 1 cut(s) 141
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 4 cut(s) 16, 268, 355, 394
LguI GCTCTTC 1 cut(s) 933
LmnI GCTCC 1 cut(s) 144
Lsp1109I GCAGC 2 cut(s) 590, 612
LweI GCATC 1 cut(s) 790
MaeI CTAG 1 cut(s) 408
MaeII ACGT 1 cut(s) 649
MaeIII GTNAC 1 cut(s) 239
MalI GATC 4 cut(s) 18, 270, 357, 396
MboI GATC 4 cut(s) 16, 268, 355, 394
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 6 cut(s) 428, 658, 680, 743, 787, 853
MlyI GAGTC 1 cut(s) 284
MnlI CCTC 5 cut(s) 322, 589, 678, 742, 829
MroI TCCGGA 1 cut(s) 141
MseI TTAA 1 cut(s) 828
MslI CAYNNNNRTG 1 cut(s) 701
MspA1I CMGCKG 1 cut(s) 603
MspI CCGG 2 cut(s) 89, 142
MspR9I CCNGG 1 cut(s) 325
MvaI CCWGG 1 cut(s) 325
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 380, 584
NdeI CATATG 1 cut(s) 232
NdeII GATC 4 cut(s) 16, 268, 355, 394
NlaIII CATG 3 cut(s) 35, 441, 706
NruI TCGCGA 1 cut(s) 288
NspV TTCGAA 1 cut(s) 426
PagI TCATGA 1 cut(s) 702
PciSI GCTCTTC 1 cut(s) 933
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PfeI GAWTC 2 cut(s) 38, 97
PflMI CCANNNNNTGG 1 cut(s) 61
PkrI GCNGC 2 cut(s) 602, 605
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
Psp6I CCWGG 1 cut(s) 323
PspGI CCWGG 1 cut(s) 323
PsuI RGATCY 1 cut(s) 16
PvuII CAGCTG 1 cut(s) 603
RruI TCGCGA 1 cut(s) 288
RseI CAYNNNNRTG 1 cut(s) 701
SapI GCTCTTC 1 cut(s) 933
SaqAI TTAA 1 cut(s) 828
SatI GCNGC 2 cut(s) 601, 604
Sau3AI GATC 4 cut(s) 16, 268, 355, 394
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 325
SduI GDGCHC 1 cut(s) 149
SfaNI GCATC 1 cut(s) 790
SfcI CTRYAG 1 cut(s) 627
SfuI TTCGAA 1 cut(s) 426
SmiMI CAYNNNNRTG 1 cut(s) 701
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
Sse9I AATT 6 cut(s) 428, 658, 680, 743, 787, 853
SsiI CCGC 2 cut(s) 598, 918
SspMI CTAG 1 cut(s) 408
StyD4I CCNGG 1 cut(s) 323
TaaI ACNGT 3 cut(s) 11, 151, 575
TaiI ACGT 1 cut(s) 652
TaqI TCGA 8 cut(s) 207, 316, 358, 426, 570, 615, 663, 798
TasI AATT 6 cut(s) 428, 658, 680, 743, 787, 853
TfiI GAWTC 2 cut(s) 38, 97
Tru1I TTAA 1 cut(s) 828
Tru9I TTAA 1 cut(s) 828
TscAI CASTG 2 cut(s) 16, 465
TseI GCWGC 2 cut(s) 600, 603
TspRI CASTG 2 cut(s) 16, 465
Van91I CCANNNNNTGG 1 cut(s) 61
XagI CCTNNNNNAGG 1 cut(s) 513
XapI RAATTY 2 cut(s) 658, 743
XmiI GTMKAC 1 cut(s) 928
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.