FvH4_6g10000

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000079
Physical Location & Seq
Forward (+)
5934810 .. 5939270
4461 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10000.t1

Sequence Viewer

Length: 2727 bp
ATGGGTTCGAACTCAAAGCGTAGTAAACGATGCGCTGAAGATAGAATCAGTGGGTTGCTAGATGCAATACTCTGTCACATTCTGTCATTCCGTTCAACAGTTGAGGCTGTAAAGACCAGTGTTTTATCACATAGATGGGAGAATTTGTGGGCTTCTGTCCCCACTATTGACGTATGTGACTATGATCCAAAAGAATTCGACTTTGATAGTTTTACAATGTTTCTTGATCGTGTACTTCTCGCTCATGACTCCTTAAAAGTTCATCGGTTCCGTATTTGCTGTACTAAGATGGTGGATCCCCTTCTGTTTGATTATTGGGTTAGCACTGCCATTGGCTGTAATGTTGTTGAACTCGATCTTGATATTTATCCCTTGGGTCCTGAGCACTCATCACTTCCACCCCAGTGTTTTGAGTTGCCAGGAAGCCTTTTCATGCTGAAAAGTTTGGAGAAATTGAAGCTGAGCCTGAAATCGTTCGTTACCATTAAACCCGAACCAAGTTGGTTCCCAAGTCTCAAGTTTCTCCATGTTTCAGTCGCATATGCCAGCTCTCATCTGATGGAGAACCTGTTTACTTGCTGCCCTGCACTTGAGGAGTTGGCATTTGAAGCAGAACCTGAATATGATGATGATTCTATTTTTAATATGAACATCTGTGCACCGAAGCTGAAACGACTAGAAATAAGCTTCTTCCTGGATAGACATGATCTAGAGAAACTTGAATATGAGCACAAACTTTTTATCAATGCCAATGCTCCTAATCTTGAAAAGTTCACTTTTTACGGCAATCTTTTGGCAATCTATATCTCAAACAATGCAAAATCCCTGAATGAAGCCAAGATTGATTGCACGGACCTGCATGCGGTTCAAGAGCTTGACGATCACCTTGATGCTGCTGATAATTTGCACAGGCTTTTCACAGGAATTTTGAATGTTACATACCTATCTGTTTCAGCTCCTATTTTGGGGCACTTGGAGCTACTTCTTCACGCTTGCTCCTCTTGGCAACCATTGATTAGTTTTCTGAATGCATCGCCAAATCTGGAATCTCTTGTCTTGAAAAAGAGCCGCGATTGCCTATGTCGACACGATCCGGATGAACTACATCATGAATGGAATCCACCAGAGTATGTGCCTGTTTGTTTATCGTCACAATTGAAGAGTATTTGCATACGAGGGTACAAGGGGACGCCAGATCAGATGAAAGTGGCAGAATATTTGTTGAATCATGGTGAGGTTTTGAACAAGGCATTGAGGCCCATCCTTGTAGCCCAAGCTCCATTGACACAAAAACCTTTCCTCACCATTTTTTCCAAGACATTTCTGATTTCTCTCTCCCTCGCCAGTTTACCTATTCGAGGGCTCTTGTTCTTCCTCAGCGGCCACACACAGTGTACCGGAACCCTAAATCCTGAAATCAGGACATTGTATCACATGGAAATGGGTTCAAGCTCAAAGCATAGTAAACGATGCGCTGAAGATAGACTCAGTCAATTGCCAGATGCAATTCTTTGCCACATTCTGTCTTTCCTTTCAACAGTTGAGGCTGTGAAAACCTGTGTTTTATCACATAGGTGGGAGAATGTGTGGGGTGCTGTGCCCACTATAGACATATCTGATCATGATCGTGATGGAAAAGAATCCGACTTTGATAGTTTTTCGGTGTTTATTGATCGTGTACTTTCCACTCATGAGTCTGTGAAAGTTCATCTGTTCCGTCTTGGCTGTTTTAGCATGGTGGATCCCCTTCGGTTTGATTATTGGGTTCGCACTGCCATTGGCTGTAATGTTGTTCAACTCGATCTTGGTATCTTTCCACCCCAGAGTTTCGAGTTGCCAGGAACCCTTTTCACGCTGAAAATCACATTTGCTAGTTCTCACTTGATGGAGAATTTCTTTTCCTGCTGTCCTGTACTTGAAGAGTTGATTTTTGATGCATGGGAATATGATGGTGATTCGGATTTTAATATTAACATCTCTGCGCCGAAGCTGAAAATACTACAAATAAGGTTGTTCCAGAAATTATATGATCACGGGCACAAACTTTTTATCAATGCCTATGCTCCTAATCTTGAAAAGTTCACTTTCAAAGGCCATCTTTTGGTAATCTACACCTTGAGCAATGCAAAATCCCTGAACGAAGCAAAGATTGATTGTAATACGCTGCATGCAGGTCAAGAGCTTGACTATCACCTTGCTGATCATTTGCACAGGCTATTGAAAGGACTTTTGAATGTTACACACCTGTCAGTTACAGCTCCTATTTTTGGGGATCCCCACATTGTTCATCAGTATCCTCTGCCTACATTTGTTAATTTGATGAACTTGGAGCTACATCTTCAAGCTTGCTGCTCTTGGCAATCATTCTTTAGTTTTCTGAATGCATCGCCAAATCTGATATCTCTTGTCTTGGAAAAGAGCCATGATTGCCTACGTTTACACTATCTTGATGAAGTATACCATGAATGGAATCCACCAGAGTGTGTGCCTGCTTGTTTGGTGTCACGCTTGAAGCTTATTTGCATAATGGATTACAGGGGGACGCCAGATCAGATGAAAATGGCAGAATACTTATTGAAGCATGGTGAGGTTTTGAGTAAGGTGATAGTGCATTGGGAAAGTTTTTCTTCCAAAAAAGAGAAGAAATTATGGCGGGGATTAAAGAAGTTGCCAAGGGGTTCAAAGACTTGTCAAATGGTGTTTCCATGCCTCTCTGTCGCAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

909

Amino Acids

103.43

Weight (kDa)

6.1

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 85 - 393 2.1e-10 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 104 - 351 2.3e-09 FBD-associated F-box protein At5g56370, LRR repeats
FBD PF08387 375 - 417 1.7e-11 FBD
F-box PF00646 496 - 530 5.5e-06 F-box domain
FBD PF08387 826 - 869 7.3e-11 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000079)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32020
fragaria_vesca FvH4_2g37070 FvH4_3g17062 FvH4_3g17580 FvH4_3g17580 FvH4_3g17580 FvH4_3g17581 FvH4_3g18162 FvH4_3g20210 FvH4_3g32940 FvH4_3g33370 FvH4_3g33390 FvH4_3g33400 FvH4_3g33400 FvH4_3g33400 FvH4_3g33401 FvH4_3g33430 FvH4_4g00640 FvH4_5g02210 FvH4_5g02220 FvH4_5g02220 FvH4_5g02221 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02280 FvH4_5g02290 FvH4_5g02290 FvH4_5g08740 FvH4_5g24470 FvH4_6g07500 FvH4_6g07500 FvH4_6g07500 FvH4_6g07500 FvH4_6g08520 FvH4_6g10000 FvH4_7g28000 FvH4_7g28011 FvH4_7g28030 FvH4_7g28040 FvH4_7g28042
malus_domestica MD03G1112000.v1.1 MD03G1112100.v1.1 MD03G1112200.v1.1 MD03G1253000.v1.1 MD03G1253100.v1.1 MD03G1253400.v1.1 MD03G1255400.v1.1 MD03G1276300.v1.1 MD03G1276400.v1.1 MD05G1193700.v1.1 MD11G1265000.v1.1 MD11G1265100.v1.1 MD11G1265300.v1.1 MD11G1275400.v1.1 MD11G1275600.v1.1
prunus_persica Prupe.4G151700_v2.0.a1 Prupe.4G160800_v2.0.a1 Prupe.4G160900_v2.0.a1 Prupe.4G161000_v2.0.a1 Prupe.4G161100_v2.0.a1 Prupe.4G161200_v2.0.a1 Prupe.4G161300_v2.0.a1 Prupe.5G021800_v2.0.a1
pyrus_communis pycom03g06140 pycom03g19340 pycom03g20150 pycom03g20210 pycom05g18110 pycom11g23320 pycom11g24360 pycom11g24380
rosa_chinensis RchiOBHm_Chr1g0321391 RchiOBHm_Chr1g0363171 RchiOBHm_Chr1g0363181 RchiOBHm_Chr2g0115721 RchiOBHm_Chr3g0458571 RchiOBHm_Chr3g0460681 RchiOBHm_Chr4g0387591 RchiOBHm_Chr5g0028191 RchiOBHm_Chr5g0028201 RchiOBHm_Chr5g0028241 RchiOBHm_Chr5g0028261 RchiOBHm_Chr5g0028411 RchiOBHm_Chr5g0028431 RchiOBHm_Chr5g0029291 RchiOBHm_Chr5g0030601 RchiOBHm_Chr5g0030631 RchiOBHm_Chr5g0059941 RchiOBHm_Chr5g0059951 RchiOBHm_Chr5g0059961 RchiOBHm_Chr5g0060061 RchiOBHm_Chr5g0060761 RchiOBHm_Chr5g0060771 RchiOBHm_Chr6g0304711 RchiOBHm_Chr7g0192751 RchiOBHm_Chr7g0197861 RchiOBHm_Chr7g0197881 RchiOBHm_Chr7g0197891 RchiOBHm_Chr7g0197921
rosa_laevigata RLG00000003903 RLG00000003904 RLG00000004332 RLG00000010109 RLG00000010957 RLG00000018203 RLG00000024989 RLG00000025147 RLG00000027586 RLG00000030426 RLG00000033074 RLG00000033075 RLG00000033076 RLG00000033094 RLG00000033150 RLG00000033234 RLG00000035345 RLG00000035399
rosa_multiflora Rmu_co8271835.1_g000001 Rmu_co8353127.1_g000001 Rmu_co8434667.1_g000001 Rmu_co8434713.1_g000001 Rmu_co8441243.1_g000001 Rmu_co8501607.1_g000001 Rmu_sc0000471.1_g000010 Rmu_sc0000724.1_g000008 Rmu_sc0000727.1_g000033 Rmu_sc0000814.1_g000032 Rmu_sc0000960.1_g000003 Rmu_sc0001082.1_g000014 Rmu_sc0001082.1_g000016 Rmu_sc0001571.1_g000005 Rmu_sc0001571.1_g000007 Rmu_sc0001571.1_g000008 Rmu_sc0001571.1_g000020 Rmu_sc0002040.1_g000017 Rmu_sc0002925.1_g000003 Rmu_sc0003864.1_g000006 Rmu_sc0005482.1_g000001 Rmu_sc0005482.1_g000005 Rmu_sc0006771.1_g000006 Rmu_sc0007143.1_g000008 Rmu_sc0007248.1_g000006 Rmu_sc0011593.1_g000004 Rmu_sc0011593.1_g000006 Rmu_sc0011593.1_g000007 Rmu_sc0011593.1_g000012 Rmu_sc0016104.1_g000007 Rmu_sc0022819.1_g000001 Rmu_sc0025685.1_g000002 Rmu_sc0027256.1_g000002 Rmu_sc0034563.1_g000001 Rmu_sc0034642.1_g000001 Rmu_ssc0000197.1_g000059
rosa_roxburghii Rroxscaffold_1G00019950 Rroxscaffold_1G00020580 Rroxscaffold_1G00051250 Rroxscaffold_1G00051270 Rroxscaffold_1G00051470 Rroxscaffold_1G00051480 Rroxscaffold_2G00127930 Rroxscaffold_2G00128790 Rroxscaffold_3G00258110 Rroxscaffold_3G00258130 Rroxscaffold_6G00419310 Rroxscaffold_6G00420970 Rroxscaffold_6G00421360 Rroxscaffold_7G00163570
rosa_rugosa Rorug01G0032500 Rorug02G0199500.1 Rorug02G0199600.1 Rorug03G0024200 Rorug03G0029800 Rorug03G0029900 Rorug03G0045000 Rorug03G0045000 Rorug03G0310900 Rorug05G0104800 Rorug05G0104900 Rorug05G0105000 Rorug05G0105100 Rorug05G0105200 Rorug05G0105300 Rorug05G0107400 Rorug05G0107600 Rorug05G0112900 Rorug05G0118900 Rorug05G0327000 Rorug05G0327000 Rorug05G0327000 Rorug05G0328000 Rorug05G0333900 Rorug06G0333700 Rorug07G0004400 Rorug07G0044100 Rorug07G0044200 Rorug07G0044200 Rorug07G0044300 Rorug07G0044400
rosa_samantha Rh1AG314000 Rh1BG277500 Rh1CG293900 Rh1DG051800 Rh1DG308100 Rh1DG308200 Rh2AG256700 Rh2AG295900 Rh2BG267300 Rh2CG262300 Rh2DG265100 Rh2DG319900 Rh3AG084700 Rh3AG088100 Rh3AG102600 Rh3BG087500 Rh3CG088000 Rh3CG091300 Rh3CG108200 Rh3DG088400 Rh4AG017000 Rh4CG018100 Rh4DG012700 Rh5AG198100 Rh5AG198200 Rh5AG198300 Rh5AG198500 Rh5AG198600 Rh5AG198700 Rh5AG200400 Rh5AG205000 Rh5AG211100 Rh5AG244900 Rh5AG391100 Rh5AG391200 Rh5AG396500 Rh5BG197000 Rh5BG197100 Rh5BG197200 Rh5BG197300 Rh5BG198600 Rh5BG198800 Rh5BG403400 Rh5BG403500 Rh5CG216700 Rh5CG216900 Rh5CG217000 Rh5CG218500 Rh5CG225000 Rh5CG427400 Rh5CG427500 Rh5CG433300 Rh5DG198400 Rh5DG198500 Rh5DG198800 Rh5DG198900 Rh5DG199000 Rh5DG200700 Rh5DG201000 Rh5DG207200 Rh5DG215000 Rh5DG374300 Rh5DG419000 Rh5DG419100 Rh5DG420200 Rh5DG423900 Rh6BG456100 Rh6CG458700 Rh7AG129400 Rh7AG168100 Rh7AG457700 Rh7BG170900 Rh7CG177400 Rh7CG177500 Rh7DG131600 Rh7DG169800 Rh7DG169900 Rh7DG170000 Rh7DG170100
rosa_wichuraiana Rw1G027820 Rw2G020090 Rw3G007530 Rw3G008730 Rw4G001080 Rw5G018080 Rw5G018090 Rw5G018100 Rw5G018110 Rw5G018120 Rw5G018640 Rw5G019310 Rw5G036810 Rw5G036820 Rw5G036830 Rw5G037390 Rw6G038770 Rw7G011070 Rw7G014650 Rw7G014660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 864, 2162
AccB7I CCANNNNNTGG 1 cut(s) 2101
AccI GTMKAC 2 cut(s) 1084, 2457
AccII CGCG 1 cut(s) 1071
AccIII TCCGGA 1 cut(s) 1093
AciI CCGC 4 cut(s) 863, 1069, 1380, 2653
AclWI GGATC 8 cut(s) 179, 290, 303, 1085, 1736, 1749, 2267, 2280
AcoI YGGCCR 1 cut(s) 1381
AcsI RAATTY 4 cut(s) 142, 194, 924, 1891
AcuI CTGAAG 2 cut(s) 57, 1497
AcyI GRCGYC 2 cut(s) 1190, 2543
AdeI CACNNNGTG 1 cut(s) 1392
AfaI GTAC 6 cut(s) 234, 283, 1181, 1396, 1680, 1914
AfiI CCNNNNNNNGG 5 cut(s) 862, 965, 1358, 2101, 2267
AjnI CCWGG 3 cut(s) 418, 693, 1837
AleI CACNNNNGTG 3 cut(s) 403, 1573, 2479
AloI GAACNNNNNNTCC 2 cut(s) 1393, 1425
Alw21I GWGCWC 3 cut(s) 387, 661, 732
Alw26I GTCTC 1 cut(s) 518
Alw44I GTGCAC 1 cut(s) 657
AlwI GGATC 8 cut(s) 179, 290, 303, 1085, 1736, 1749, 2267, 2280
AlwNI CAGNNNCTG 1 cut(s) 617
Aor13HI TCCGGA 1 cut(s) 1093
AoxI GGCC 3 cut(s) 1256, 1381, 2092
ApaLI GTGCAC 1 cut(s) 657
ApeKI GCWGC 5 cut(s) 579, 893, 2164, 2349, 2720
ApoI RAATTY 4 cut(s) 142, 194, 924, 1891
Asp700I GAANNNNTTC 1 cut(s) 473
AspLEI GCGC 3 cut(s) 35, 1475, 1984
AspS9I GGNCC 3 cut(s) 377, 853, 1257
AsuHPI GGTGA 7 cut(s) 875, 1244, 1294, 1964, 2183, 2597, 2614
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 2 cut(s) 377, 853
BaeGI GKGCMC 4 cut(s) 661, 972, 1602, 2040
BamHI GGATCC 3 cut(s) 295, 1741, 2272
BanII GRGCYC 1 cut(s) 1365
Bbv12I GWGCWC 3 cut(s) 387, 661, 732
BbvCI CCTCAGC 1 cut(s) 1376
BbvI GCAGC 4 cut(s) 566, 880, 2151, 2336
BccI CCATC 8 cut(s) 129, 283, 553, 1268, 1625, 1879, 1943, 2103
BceAI ACGGC 1 cut(s) 799
BciT130I CCWGG 3 cut(s) 420, 695, 1839
BciVI GTATCC 1 cut(s) 2304
BclI TGATCA 3 cut(s) 1618, 2029, 2200
BcoDI GTCTC 1 cut(s) 518
BfaI CTAG 4 cut(s) 59, 677, 710, 1872
BfmI CTRYAG 1 cut(s) 1605
BfuAI ACCTGC 2 cut(s) 864, 2162
BfuI GTATCC 1 cut(s) 2304
BisI GCNGC 7 cut(s) 580, 894, 1069, 1381, 2165, 2350, 2721
BlpI GCTNAGC 1 cut(s) 461
BlsI GCNGC 7 cut(s) 581, 895, 1070, 1382, 2166, 2351, 2722
Bme1390I CCNGG 3 cut(s) 420, 695, 1839
Bme18I GGWCC 2 cut(s) 377, 853
BmgT120I GGNCC 3 cut(s) 377, 853, 1257
BmiI GGNNCC 8 cut(s) 269, 297, 378, 506, 1402, 1743, 1843, 2274
BmrFI CCNGG 3 cut(s) 420, 695, 1839
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 8 cut(s) 20, 52, 880, 1040, 1460, 1492, 1924, 2393
BmuI ACTGGG 1 cut(s) 397
Bpu10I CCTNAGC 2 cut(s) 381, 1376
Bpu1102I GCTNAGC 1 cut(s) 461
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 3 cut(s) 500, 611, 2137
BsaBI GATNNNNATC 2 cut(s) 366, 1623
BsaHI GRCGYC 2 cut(s) 1190, 2543
BsaJI CCNNGG 2 cut(s) 372, 2672
BsaWI WCCGGW 2 cut(s) 1093, 1397
BsaXI ACNNNNNCTCC 8 cut(s) 131, 161, 554, 584, 980, 1010, 1571, 1601
Bsc4I CCNNNNNNNGG 5 cut(s) 862, 965, 1358, 2101, 2267
Bse1I ACTGG 3 cut(s) 117, 403, 1344
Bse3DI GCAATG 1 cut(s) 2128
Bse8I GATNNNNATC 2 cut(s) 366, 1623
BseAI TCCGGA 1 cut(s) 1093
BseBI CCWGG 3 cut(s) 420, 695, 1839
BseDI CCNNGG 2 cut(s) 372, 2672
BseGI GGATG 2 cut(s) 1102, 1260
BseJI GATNNNNATC 2 cut(s) 366, 1623
BseLI CCNNNNNNNGG 5 cut(s) 862, 965, 1358, 2101, 2267
BseMI GCAATG 1 cut(s) 2128
BseMII CTCAG 4 cut(s) 372, 452, 1390, 1501
BseNI ACTGG 3 cut(s) 117, 403, 1344
BseRI GAGGAG 2 cut(s) 608, 988
BseSI GKGCMC 4 cut(s) 661, 972, 1602, 2040
BseXI GCAGC 4 cut(s) 566, 880, 2151, 2336
BsgI GTGCAG 1 cut(s) 570
Bsh1236I CGCG 1 cut(s) 1071
BshFI GGCC 3 cut(s) 1258, 1383, 2094
BsiHKAI GWGCWC 3 cut(s) 387, 661, 732
BsiSI CCGG 2 cut(s) 1094, 1398
BslFI GGGAC 3 cut(s) 143, 1201, 2554
BslI CCNNNNNNNGG 5 cut(s) 862, 965, 1358, 2101, 2267
BsmAI GTCTC 1 cut(s) 518
BsmFI GGGAC 3 cut(s) 143, 1201, 2554
BsmI GAATGC 2 cut(s) 1033, 2386
BsnI GGCC 3 cut(s) 1258, 1383, 2094
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 7 cut(s) 387, 661, 732, 972, 1365, 1602, 2040
Bsp13I TCCGGA 1 cut(s) 1093
Bsp1720I GCTNAGC 1 cut(s) 461
BspACI CCGC 4 cut(s) 863, 1069, 1380, 2653
BspANI GGCC 3 cut(s) 1258, 1383, 2094
BspCNI CTCAG 4 cut(s) 373, 453, 1389, 1500
BspEI TCCGGA 1 cut(s) 1093
BspFNI CGCG 1 cut(s) 1071
BspHI TCATGA 4 cut(s) 244, 1108, 1621, 1690
BspLI GGNNCC 8 cut(s) 269, 297, 378, 506, 1402, 1743, 1843, 2274
BspMI ACCTGC 2 cut(s) 864, 2162
BspPI GGATC 8 cut(s) 179, 290, 303, 1085, 1736, 1749, 2267, 2280
BspT104I TTCGAA 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 2128
BsrI ACTGG 3 cut(s) 117, 403, 1344
BssECI CCNNGG 2 cut(s) 372, 2672
BssNAI GTATAC 1 cut(s) 2458
BssNI GRCGYC 2 cut(s) 1190, 2543
BssT1I CCWWGG 2 cut(s) 372, 2672
Bst1107I GTATAC 1 cut(s) 2458
Bst2UI CCWGG 3 cut(s) 420, 695, 1839
Bst4CI ACNGT 3 cut(s) 100, 1392, 1540
Bst6I CTCTTC 2 cut(s) 1154, 1914
BstACI GRCGYC 2 cut(s) 1190, 2543
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 6 cut(s) 547, 861, 994, 2169, 2347, 2490
BstDEI CTNAG 5 cut(s) 285, 381, 461, 1376, 1487
BstENI CCTNNNNNAGG 1 cut(s) 1356
BstF5I GGATG 2 cut(s) 1102, 1260
BstFNI CGCG 1 cut(s) 1071
BstHHI GCGC 3 cut(s) 35, 1475, 1984
BstMAI GTCTC 1 cut(s) 518
BstMWI GCNNNNNNNGC 5 cut(s) 608, 976, 1074, 1731, 2427
BstNI CCWGG 3 cut(s) 420, 695, 1839
BstNSI RCATGY 2 cut(s) 863, 2171
BstSCI CCNGG 3 cut(s) 418, 693, 1837
BstSFI CTRYAG 1 cut(s) 1605
BstSLI GKGCMC 4 cut(s) 661, 972, 1602, 2040
BstUI CGCG 1 cut(s) 1071
BstV1I GCAGC 4 cut(s) 566, 880, 2151, 2336
BstX2I RGATCY 3 cut(s) 295, 1741, 2272
BstYI RGATCY 3 cut(s) 295, 1741, 2272
BstZ17I GTATAC 1 cut(s) 2458
BsuI GTATCC 1 cut(s) 2304
BsuRI GGCC 3 cut(s) 1258, 1383, 2094
BtgZI GCGATG 2 cut(s) 1017, 2370
BtsCI GGATG 2 cut(s) 1102, 1260
BtsI GCAGTG 2 cut(s) 324, 1770
BtsIMutI CAGTG 6 cut(s) 55, 124, 324, 410, 1397, 1770
BveI ACCTGC 2 cut(s) 864, 2162
Cac8I GCNNGC 6 cut(s) 547, 861, 994, 2169, 2347, 2490
CaiI CAGNNNCTG 1 cut(s) 617
CciI TCATGA 4 cut(s) 244, 1108, 1621, 1690
CfoI GCGC 3 cut(s) 35, 1475, 1984
Cfr13I GGNCC 3 cut(s) 377, 853, 1257
CseI GACGC 2 cut(s) 1198, 2551
Csp6I GTAC 6 cut(s) 233, 282, 1180, 1395, 1679, 1913
CviQI GTAC 6 cut(s) 233, 282, 1180, 1395, 1679, 1913
DdeI CTNAG 5 cut(s) 285, 381, 461, 1376, 1487
DraIII CACNNNGTG 1 cut(s) 1392
EaeI YGGCCR 1 cut(s) 1381
Eam1104I CTCTTC 2 cut(s) 1154, 1914
EarI CTCTTC 2 cut(s) 1154, 1914
Eco130I CCWWGG 2 cut(s) 372, 2672
Eco24I GRGCYC 1 cut(s) 1365
Eco32I GATATC 1 cut(s) 2400
Eco47I GGWCC 2 cut(s) 377, 853
Eco57I CTGAAG 2 cut(s) 57, 1497
EcoNI CCTNNNNNAGG 1 cut(s) 1356
EcoO109I RGGNCCY 1 cut(s) 377
EcoRI GAATTC 1 cut(s) 194
EcoRII CCWGG 3 cut(s) 418, 693, 1837
EcoRV GATATC 1 cut(s) 2400
EcoT14I CCWWGG 2 cut(s) 372, 2672
EcoT22I ATGCAT 3 cut(s) 1033, 1939, 2386
EcoT38I GRGCYC 1 cut(s) 1365
ErhI CCWWGG 2 cut(s) 372, 2672
FalI AAGNNNNNCTT 4 cut(s) 2082, 2114, 2611, 2643
FaqI GGGAC 3 cut(s) 143, 1201, 2554
FauI CCCGC 1 cut(s) 2646
FauNDI CATATG 1 cut(s) 541
FbaI TGATCA 3 cut(s) 1618, 2029, 2200
FblI GTMKAC 2 cut(s) 1084, 2457
Fnu4HI GCNGC 7 cut(s) 580, 894, 1069, 1381, 2165, 2350, 2721
FokI GGATG 2 cut(s) 1109, 1247
FriOI GRGCYC 1 cut(s) 1365
Fsp4HI GCNGC 7 cut(s) 580, 894, 1069, 1381, 2165, 2350, 2721
FspBI CTAG 4 cut(s) 59, 677, 710, 1872
GlaI GCGC 3 cut(s) 34, 1474, 1983
GluI GCNGC 7 cut(s) 580, 894, 1069, 1381, 2165, 2350, 2721
HaeIII GGCC 3 cut(s) 1258, 1383, 2094
HapII CCGG 2 cut(s) 1094, 1398
HgaI GACGC 2 cut(s) 1198, 2551
HhaI GCGC 3 cut(s) 35, 1475, 1984
Hin1I GRCGYC 2 cut(s) 1190, 2543
Hin6I GCGC 3 cut(s) 33, 1473, 1982
HinP1I GCGC 3 cut(s) 33, 1473, 1982
HincII GTYRAC 1 cut(s) 1085
HindII GTYRAC 1 cut(s) 1085
HindIII AAGCTT 3 cut(s) 685, 2343, 2513
HpaII CCGG 2 cut(s) 1094, 1398
HphI GGTGA 7 cut(s) 875, 1244, 1294, 1964, 2183, 2597, 2614
HpyAV CCTTC 2 cut(s) 311, 1757
HpyCH4III ACNGT 3 cut(s) 100, 1392, 1540
HpyCH4IV ACGT 2 cut(s) 171, 2434
HpyF10VI GCNNNNNNNGC 5 cut(s) 608, 976, 1074, 1731, 2427
HpyF3I CTNAG 5 cut(s) 285, 381, 461, 1376, 1487
HpySE526I ACGT 2 cut(s) 171, 2434
Hsp92I GRCGYC 2 cut(s) 1190, 2543
HspAI GCGC 3 cut(s) 33, 1473, 1982
Kpn2I TCCGGA 1 cut(s) 1093
Ksp22I TGATCA 3 cut(s) 1618, 2029, 2200
LmnI GCTCC 8 cut(s) 760, 961, 976, 1001, 1282, 2068, 2263, 2329
Lsp1109I GCAGC 4 cut(s) 566, 880, 2151, 2336
LweI GCATC 8 cut(s) 20, 52, 880, 1040, 1460, 1492, 1924, 2393
MaeI CTAG 4 cut(s) 59, 677, 710, 1872
MaeII ACGT 2 cut(s) 171, 2434
MaeIII GTNAC 8 cut(s) 74, 176, 478, 934, 1149, 2236, 2251, 2502
MfeI CAATTG 2 cut(s) 1154, 1493
MflI RGATCY 3 cut(s) 295, 1741, 2272
MhlI GDGCHC 7 cut(s) 387, 661, 732, 972, 1365, 1602, 2040
MlyI GAGTC 3 cut(s) 242, 1479, 1703
MmeI TCCRAC 1 cut(s) 1668
Mph1103I ATGCAT 3 cut(s) 1033, 1939, 2386
MroI TCCGGA 1 cut(s) 1093
MroXI GAANNNNTTC 1 cut(s) 473
MseI TTAA 7 cut(s) 254, 486, 642, 1965, 1971, 2313, 2660
MslI CAYNNNNRTG 7 cut(s) 133, 403, 888, 1439, 1573, 1626, 2479
MspA1I CMGCKG 1 cut(s) 1380
MspI CCGG 2 cut(s) 1094, 1398
MspR9I CCNGG 3 cut(s) 420, 695, 1839
MunI CAATTG 2 cut(s) 1154, 1493
Mva1269I GAATGC 2 cut(s) 1033, 2386
MvaI CCWGG 3 cut(s) 420, 695, 1839
MvnI CGCG 1 cut(s) 1071
MwoI GCNNNNNNNGC 5 cut(s) 608, 976, 1074, 1731, 2427
NdeI CATATG 1 cut(s) 541
NlaIV GGNNCC 8 cut(s) 269, 297, 378, 506, 1402, 1743, 1843, 2274
NmuCI GTSAC 4 cut(s) 74, 176, 1149, 2502
NsiI ATGCAT 3 cut(s) 1033, 1939, 2386
NspI RCATGY 2 cut(s) 863, 2171
NspV TTCGAA 1 cut(s) 8
OliI CACNNNNGTG 3 cut(s) 403, 1573, 2479
PaeI GCATGC 2 cut(s) 863, 2171
PagI TCATGA 4 cut(s) 244, 1108, 1621, 1690
PctI GAATGC 2 cut(s) 1033, 2386
PdmI GAANNNNTTC 1 cut(s) 473
PfeI GAWTC 8 cut(s) 45, 632, 1046, 1117, 1225, 1640, 1955, 2470
PflFI GACNNNGTC 1 cut(s) 1488
PflMI CCANNNNNTGG 1 cut(s) 2101
PfoI TCCNGGA 1 cut(s) 693
PkrI GCNGC 7 cut(s) 581, 895, 1070, 1382, 2166, 2351, 2722
PleI GAGTC 3 cut(s) 242, 1479, 1702
PpsI GAGTC 3 cut(s) 242, 1479, 1702
PpuMI RGGWCCY 1 cut(s) 377
Psp5II RGGWCCY 1 cut(s) 377
Psp6I CCWGG 3 cut(s) 418, 693, 1837
PspGI CCWGG 3 cut(s) 418, 693, 1837
PspN4I GGNNCC 8 cut(s) 269, 297, 378, 506, 1402, 1743, 1843, 2274
PspPI GGNCC 3 cut(s) 377, 853, 1257
PspPPI RGGWCCY 1 cut(s) 377
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 3 cut(s) 295, 1741, 2272
PsyI GACNNNGTC 1 cut(s) 1488
RsaI GTAC 6 cut(s) 234, 283, 1181, 1396, 1680, 1914
RsaNI GTAC 6 cut(s) 233, 282, 1180, 1395, 1679, 1913
RseI CAYNNNNRTG 7 cut(s) 133, 403, 888, 1439, 1573, 1626, 2479
SalI GTCGAC 1 cut(s) 1083
SaqAI TTAA 7 cut(s) 254, 486, 642, 1965, 1971, 2313, 2660
SatI GCNGC 7 cut(s) 580, 894, 1069, 1381, 2165, 2350, 2721
Sau96I GGNCC 3 cut(s) 377, 853, 1257
SchI GAGTC 3 cut(s) 242, 1479, 1703
ScrFI CCNGG 3 cut(s) 420, 695, 1839
SduI GDGCHC 7 cut(s) 387, 661, 732, 972, 1365, 1602, 2040
SfaNI GCATC 8 cut(s) 20, 52, 880, 1040, 1460, 1492, 1924, 2393
SfcI CTRYAG 1 cut(s) 1605
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 2 cut(s) 377, 853
SmiMI CAYNNNNRTG 7 cut(s) 133, 403, 888, 1439, 1573, 1626, 2479
SmlI CTYRAG 3 cut(s) 515, 590, 2116
SmoI CTYRAG 3 cut(s) 515, 590, 2116
SphI GCATGC 2 cut(s) 863, 2171
SsiI CCGC 4 cut(s) 863, 1069, 1380, 2653
SspI AATATT 2 cut(s) 1217, 1969
SspMI CTAG 4 cut(s) 59, 677, 710, 1872
StyD4I CCNGG 3 cut(s) 418, 693, 1837
StyI CCWWGG 2 cut(s) 372, 2672
TaaI ACNGT 3 cut(s) 100, 1392, 1540
TaiI ACGT 2 cut(s) 174, 2437
TaqI TCGA 7 cut(s) 8, 198, 354, 1084, 1357, 1800, 1830
TatI WGTACW 4 cut(s) 232, 281, 1678, 1912
TauI GCSGC 2 cut(s) 1071, 1383
TfiI GAWTC 8 cut(s) 45, 632, 1046, 1117, 1225, 1640, 1955, 2470
Tru1I TTAA 7 cut(s) 254, 486, 642, 1965, 1971, 2313, 2660
Tru9I TTAA 7 cut(s) 254, 486, 642, 1965, 1971, 2313, 2660
TscAI CASTG 6 cut(s) 55, 124, 331, 410, 1397, 1777
TseFI GTSAC 4 cut(s) 74, 176, 1149, 2502
TseI GCWGC 5 cut(s) 579, 893, 2164, 2349, 2720
Tsp45I GTSAC 4 cut(s) 74, 176, 1149, 2502
TspGWI ACGGA 4 cut(s) 80, 260, 866, 1706
TspRI CASTG 6 cut(s) 55, 124, 331, 410, 1397, 1777
Tth111I GACNNNGTC 1 cut(s) 1488
Van91I CCANNNNNTGG 1 cut(s) 2101
VneI GTGCAC 1 cut(s) 657
VpaK11BI GGWCC 2 cut(s) 377, 853
XagI CCTNNNNNAGG 1 cut(s) 1356
XapI RAATTY 4 cut(s) 142, 194, 924, 1891
XbaI TCTAGA 1 cut(s) 709
XceI RCATGY 2 cut(s) 863, 2171
XmiI GTMKAC 2 cut(s) 1084, 2457
XmnI GAANNNNTTC 1 cut(s) 473
XspI CTAG 4 cut(s) 59, 677, 710, 1872
Zsp2I ATGCAT 3 cut(s) 1033, 1939, 2386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.