RLG00000024989

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000079
Physical Location & Seq
Reverse (-)
41110927 .. 41116602
5676 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024989

Sequence Viewer

Length: 1719 bp
ATGAATGTGAAATTAGGAATACGGGTACCTGGCGCAATTAGTGGAGACGCTGATCTTGTAAGGAATGTTGACCTTGCAGTTGGAAGGGAGCCTAGCAGCGTTGGTTGGGTTGATCCCTTGGAATTTAGCAGCAGCTGCTTTCAAGCACTTGCAAGCATTCTGGCGGTCAGCGGTGGTCCTCGCCGAGTTGACCATGTTTCTCAGGTTGGTGCAACACCCCGCACTGGGGGTCCCACCTTTGGTGAGGTAGCCCAGGCACGGTGCCATCCCGCCCTGGATCTGGTTGCATGTCATGGCCGCTTCGTTAAAAATTTAAAATGCTCCGAGTATTGCTACAGTGATCTGTATGCTCGCAAAGAAGGTGGTGGTGCTTATATAGGGGAGATGGTGGTGGCAAGTGGCAGCGTTGTGCTATTCGATGACATATCGGAGGCCGGAGTCAGCAATTGTTGTCCTACAGATCTAACCAGCGAGGTTTGGGTCTGGTTTAGAAACTTTCAGGTCCTCCGTGGGCACCACCCCAAGACTAGTGGGGCAGGCGGCGACATCTTGATTCGTGTGAATAGGGCCGGGATGAGGTTCCTCCTCCGAGGATTGGTGGCGCAACGGCTGGTTACCCATTTCGAGGAGAAGATTGGCGTAGCAACCTACAGTCCTCAGCAACCACACACAGTATCCCCCCGGAACCCTAAACCCAGAACCGACTCGACCATTCAGAAATTGTATCACATGAAAATGGGTTCGAACTCAAAGCGTCGAAAACGATGCACCGAAGATAGACTCAGTGAATTGCCAGATGCAATTCTTTGCCACATTCTGTCTTTCCTTTCAACAGTTGAGGCTGTAAAGACCAGTGTTTTATCACATAGATTGGTGAATGTGTGGGCTTCGGTGCCCACTATAGACGTATGTGATTATGATCCAAAAGAATTCGACTGCGATAGTTTTTCGATGTTTCTTGATAATGTACTTTTCGCTCATGACTCCTTGAAAGTTCATCGGTTCCGCATTTGCTGTACTAAGATGGTGGATCCCCTTCTGTTTGATTATTGGATTAGCACTGCCATTGGCTGTAATGTTGGTGAACTCGATCTTGGTATTATTCCCATGGGTTCTGTGTACTCGTCACTTCCGCCCCAGTGTTTCGAGTTGCCAGGAAGCCTTTTCGTGATGAAAAGTTTGGAGAAGTTGAAGCTGAGCCTGCAATCGTTTATCACCATTACTCCTGAACCAAATTGGTTCCCAAGTCTCAAGTTTCTCCATGTTTCAGTCTCATTTGCTGGTTCTCATCTGATGGAGAACCTCTTGTCTTGCTGCCCTATACTTGAAGAGTTGATTTTTGATGCGTACCCTGAATATGATGATGATTCAGTTTTTAACATTAACATCTCTGCACCGAAGCTGAGAAGACTACAAATAAGCTTGTTCCTGGAAACATATGATCTAGAGGTAGTAGAATATGAGCACAAACTTTTTATCAATGCCAATGCTCCAAATCTTGAAAAGTTCACTTTCGACGGCAATCTTTTGGCAATCTATTCCTCGAGCAATGCAAAATCCCTGAACGAAGCCAAGATTGATTGCGCAGACCTGCATGCGGGTCAAGAGCTTGACTATCACCTTGATGCTGCTGATAATTTGCACAGGCTTTTCACAGGAATTTTGAATGTTACATACCTGTCAGTTTCAGCTCCTATTTTTGGGGTAAGTATGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

573

Amino Acids

63.33

Weight (kDa)

5.84

Isoelectric Point (pI)

39.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 330 - 537 1.4e-07 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 349 - 509 4.3e-10 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000079)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32020
fragaria_vesca FvH4_2g37070 FvH4_3g17062 FvH4_3g17580 FvH4_3g17580 FvH4_3g17580 FvH4_3g17581 FvH4_3g18162 FvH4_3g20210 FvH4_3g32940 FvH4_3g33370 FvH4_3g33390 FvH4_3g33400 FvH4_3g33400 FvH4_3g33400 FvH4_3g33401 FvH4_3g33430 FvH4_4g00640 FvH4_5g02210 FvH4_5g02220 FvH4_5g02220 FvH4_5g02221 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02280 FvH4_5g02290 FvH4_5g02290 FvH4_5g08740 FvH4_5g24470 FvH4_6g07500 FvH4_6g07500 FvH4_6g07500 FvH4_6g07500 FvH4_6g08520 FvH4_6g10000 FvH4_7g28000 FvH4_7g28011 FvH4_7g28030 FvH4_7g28040 FvH4_7g28042
malus_domestica MD03G1112000.v1.1 MD03G1112100.v1.1 MD03G1112200.v1.1 MD03G1253000.v1.1 MD03G1253100.v1.1 MD03G1253400.v1.1 MD03G1255400.v1.1 MD03G1276300.v1.1 MD03G1276400.v1.1 MD05G1193700.v1.1 MD11G1265000.v1.1 MD11G1265100.v1.1 MD11G1265300.v1.1 MD11G1275400.v1.1 MD11G1275600.v1.1
prunus_persica Prupe.4G151700_v2.0.a1 Prupe.4G160800_v2.0.a1 Prupe.4G160900_v2.0.a1 Prupe.4G161000_v2.0.a1 Prupe.4G161100_v2.0.a1 Prupe.4G161200_v2.0.a1 Prupe.4G161300_v2.0.a1 Prupe.5G021800_v2.0.a1
pyrus_communis pycom03g06140 pycom03g19340 pycom03g20150 pycom03g20210 pycom05g18110 pycom11g23320 pycom11g24360 pycom11g24380
rosa_chinensis RchiOBHm_Chr1g0321391 RchiOBHm_Chr1g0363171 RchiOBHm_Chr1g0363181 RchiOBHm_Chr2g0115721 RchiOBHm_Chr3g0458571 RchiOBHm_Chr3g0460681 RchiOBHm_Chr4g0387591 RchiOBHm_Chr5g0028191 RchiOBHm_Chr5g0028201 RchiOBHm_Chr5g0028241 RchiOBHm_Chr5g0028261 RchiOBHm_Chr5g0028411 RchiOBHm_Chr5g0028431 RchiOBHm_Chr5g0029291 RchiOBHm_Chr5g0030601 RchiOBHm_Chr5g0030631 RchiOBHm_Chr5g0059941 RchiOBHm_Chr5g0059951 RchiOBHm_Chr5g0059961 RchiOBHm_Chr5g0060061 RchiOBHm_Chr5g0060761 RchiOBHm_Chr5g0060771 RchiOBHm_Chr6g0304711 RchiOBHm_Chr7g0192751 RchiOBHm_Chr7g0197861 RchiOBHm_Chr7g0197881 RchiOBHm_Chr7g0197891 RchiOBHm_Chr7g0197921
rosa_laevigata RLG00000003903 RLG00000003904 RLG00000004332 RLG00000010109 RLG00000010957 RLG00000018203 RLG00000024989 RLG00000025147 RLG00000027586 RLG00000030426 RLG00000033074 RLG00000033075 RLG00000033076 RLG00000033094 RLG00000033150 RLG00000033234 RLG00000035345 RLG00000035399
rosa_multiflora Rmu_co8271835.1_g000001 Rmu_co8353127.1_g000001 Rmu_co8434667.1_g000001 Rmu_co8434713.1_g000001 Rmu_co8441243.1_g000001 Rmu_co8501607.1_g000001 Rmu_sc0000471.1_g000010 Rmu_sc0000724.1_g000008 Rmu_sc0000727.1_g000033 Rmu_sc0000814.1_g000032 Rmu_sc0000960.1_g000003 Rmu_sc0001082.1_g000014 Rmu_sc0001082.1_g000016 Rmu_sc0001571.1_g000005 Rmu_sc0001571.1_g000007 Rmu_sc0001571.1_g000008 Rmu_sc0001571.1_g000020 Rmu_sc0002040.1_g000017 Rmu_sc0002925.1_g000003 Rmu_sc0003864.1_g000006 Rmu_sc0005482.1_g000001 Rmu_sc0005482.1_g000005 Rmu_sc0006771.1_g000006 Rmu_sc0007143.1_g000008 Rmu_sc0007248.1_g000006 Rmu_sc0011593.1_g000004 Rmu_sc0011593.1_g000006 Rmu_sc0011593.1_g000007 Rmu_sc0011593.1_g000012 Rmu_sc0016104.1_g000007 Rmu_sc0022819.1_g000001 Rmu_sc0025685.1_g000002 Rmu_sc0027256.1_g000002 Rmu_sc0034563.1_g000001 Rmu_sc0034642.1_g000001 Rmu_ssc0000197.1_g000059
rosa_roxburghii Rroxscaffold_1G00019950 Rroxscaffold_1G00020580 Rroxscaffold_1G00051250 Rroxscaffold_1G00051270 Rroxscaffold_1G00051470 Rroxscaffold_1G00051480 Rroxscaffold_2G00127930 Rroxscaffold_2G00128790 Rroxscaffold_3G00258110 Rroxscaffold_3G00258130 Rroxscaffold_6G00419310 Rroxscaffold_6G00420970 Rroxscaffold_6G00421360 Rroxscaffold_7G00163570
rosa_rugosa Rorug01G0032500 Rorug02G0199500.1 Rorug02G0199600.1 Rorug03G0024200 Rorug03G0029800 Rorug03G0029900 Rorug03G0045000 Rorug03G0045000 Rorug03G0310900 Rorug05G0104800 Rorug05G0104900 Rorug05G0105000 Rorug05G0105100 Rorug05G0105200 Rorug05G0105300 Rorug05G0107400 Rorug05G0107600 Rorug05G0112900 Rorug05G0118900 Rorug05G0327000 Rorug05G0327000 Rorug05G0327000 Rorug05G0328000 Rorug05G0333900 Rorug06G0333700 Rorug07G0004400 Rorug07G0044100 Rorug07G0044200 Rorug07G0044200 Rorug07G0044300 Rorug07G0044400
rosa_samantha Rh1AG314000 Rh1BG277500 Rh1CG293900 Rh1DG051800 Rh1DG308100 Rh1DG308200 Rh2AG256700 Rh2AG295900 Rh2BG267300 Rh2CG262300 Rh2DG265100 Rh2DG319900 Rh3AG084700 Rh3AG088100 Rh3AG102600 Rh3BG087500 Rh3CG088000 Rh3CG091300 Rh3CG108200 Rh3DG088400 Rh4AG017000 Rh4CG018100 Rh4DG012700 Rh5AG198100 Rh5AG198200 Rh5AG198300 Rh5AG198500 Rh5AG198600 Rh5AG198700 Rh5AG200400 Rh5AG205000 Rh5AG211100 Rh5AG244900 Rh5AG391100 Rh5AG391200 Rh5AG396500 Rh5BG197000 Rh5BG197100 Rh5BG197200 Rh5BG197300 Rh5BG198600 Rh5BG198800 Rh5BG403400 Rh5BG403500 Rh5CG216700 Rh5CG216900 Rh5CG217000 Rh5CG218500 Rh5CG225000 Rh5CG427400 Rh5CG427500 Rh5CG433300 Rh5DG198400 Rh5DG198500 Rh5DG198800 Rh5DG198900 Rh5DG199000 Rh5DG200700 Rh5DG201000 Rh5DG207200 Rh5DG215000 Rh5DG374300 Rh5DG419000 Rh5DG419100 Rh5DG420200 Rh5DG423900 Rh6BG456100 Rh6CG458700 Rh7AG129400 Rh7AG168100 Rh7AG457700 Rh7BG170900 Rh7CG177400 Rh7CG177500 Rh7DG131600 Rh7DG169800 Rh7DG169900 Rh7DG170000 Rh7DG170100
rosa_wichuraiana Rw1G027820 Rw2G020090 Rw3G007530 Rw3G008730 Rw4G001080 Rw5G018080 Rw5G018090 Rw5G018100 Rw5G018110 Rw5G018120 Rw5G018640 Rw5G019310 Rw5G036810 Rw5G036820 Rw5G036830 Rw5G037390 Rw6G038770 Rw7G011070 Rw7G014650 Rw7G014660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1585
Acc36I ACCTGC 1 cut(s) 1599
Acc65I GGTACC 1 cut(s) 25
AccB1I GGYRCC 4 cut(s) 25, 261, 513, 892
AciI CCGC 9 cut(s) 164, 171, 220, 270, 298, 540, 1006, 1133, 1598
AclWI GGATC 5 cut(s) 107, 285, 914, 1025, 1038
AcoI YGGCCR 1 cut(s) 295
AcsI RAATTY 4 cut(s) 122, 310, 929, 1659
AfaI GTAC 5 cut(s) 27, 969, 1018, 1121, 1349
AgsI TTSAA 7 cut(s) 143, 831, 991, 1192, 1328, 1502, 1666
AhlI ACTAGT 1 cut(s) 527
AjnI CCWGG 5 cut(s) 28, 252, 273, 1153, 1428
AjuI GAANNNNNNNTTGG 2 cut(s) 1077, 1109
AluBI AGCT 6 cut(s) 135, 1195, 1402, 1422, 1609, 1691
AluI AGCT 6 cut(s) 135, 1195, 1402, 1422, 1609, 1691
Alw21I GWGCWC 1 cut(s) 1467
Alw26I GTCTC 3 cut(s) 39, 1253, 1276
AlwI GGATC 5 cut(s) 107, 285, 914, 1025, 1038
AlwNI CAGNNNCTG 1 cut(s) 135
Ama87I CYCGRG 1 cut(s) 1543
AoxI GGCC 3 cut(s) 295, 432, 567
ApeKI GCWGC 7 cut(s) 96, 129, 132, 135, 402, 1314, 1628
ApoI RAATTY 4 cut(s) 122, 310, 929, 1659
Asp718I GGTACC 1 cut(s) 25
AspLEI GCGC 3 cut(s) 35, 604, 1586
AspS9I GGNCC 4 cut(s) 176, 230, 502, 567
AsuC2I CCSGG 2 cut(s) 571, 682
AsuHPI GGTGA 5 cut(s) 254, 886, 1094, 1207, 1610
AsuII TTCGAA 1 cut(s) 743
AvaI CYCGRG 1 cut(s) 1543
AvaII GGWCC 3 cut(s) 176, 230, 502
BaeGI GKGCMC 2 cut(s) 516, 897
BamHI GGATCC 1 cut(s) 1030
BanI GGYRCC 4 cut(s) 25, 261, 513, 892
BbsI GAAGAC 1 cut(s) 1414
Bbv12I GWGCWC 1 cut(s) 1467
BbvCI CCTCAGC 1 cut(s) 657
BbvI GCAGC 7 cut(s) 108, 122, 141, 144, 414, 1301, 1615
BccI CCATC 4 cut(s) 273, 379, 1018, 1288
BceAI ACGGC 2 cut(s) 623, 1534
BcgI CGANNNNNNTGC 2 cut(s) 747, 781
BciT130I CCWGG 5 cut(s) 30, 254, 275, 1155, 1430
BciVI GTATCC 1 cut(s) 685
BcnI CCSGG 2 cut(s) 571, 682
BcoDI GTCTC 3 cut(s) 39, 1253, 1276
BcuI ACTAGT 1 cut(s) 527
BfaI CTAG 3 cut(s) 93, 528, 1445
BfmI CTRYAG 4 cut(s) 334, 456, 649, 900
BfuAI ACCTGC 1 cut(s) 1599
BfuI GTATCC 1 cut(s) 685
BglII AGATCT 1 cut(s) 460
BisI GCNGC 9 cut(s) 97, 130, 133, 136, 298, 403, 541, 1315, 1629
BlpI GCTNAGC 1 cut(s) 1196
BlsI GCNGC 9 cut(s) 98, 131, 134, 137, 299, 404, 542, 1316, 1630
Bme1390I CCNGG 7 cut(s) 30, 254, 275, 571, 682, 1155, 1430
Bme18I GGWCC 3 cut(s) 176, 230, 502
BmeT110I CYCGRG 1 cut(s) 1543
BmgT120I GGNCC 4 cut(s) 176, 230, 502, 567
BmrFI CCNGG 7 cut(s) 30, 254, 275, 571, 682, 1155, 1430
BmrI ACTGGG 2 cut(s) 234, 1132
BmsI GCATC 4 cut(s) 755, 787, 1333, 1615
BmuI ACTGGG 2 cut(s) 234, 1132
BpiI GAAGAC 1 cut(s) 1414
Bpu10I CCTNAGC 1 cut(s) 657
Bpu1102I GCTNAGC 1 cut(s) 1196
Bpu14I TTCGAA 1 cut(s) 743
BpuEI CTTGAG 1 cut(s) 1235
BpuMI CCSGG 2 cut(s) 571, 682
BsaBI GATNNNNATC 1 cut(s) 918
BsaJI CCNNGG 7 cut(s) 117, 252, 273, 508, 589, 680, 1107
BsaXI ACNNNNNCTCC 2 cut(s) 1207, 1237
Bse1I ACTGG 3 cut(s) 229, 852, 1138
Bse3DI GCAATG 1 cut(s) 1555
Bse8I GATNNNNATC 1 cut(s) 918
BseBI CCWGG 5 cut(s) 30, 254, 275, 1155, 1430
BseDI CCNNGG 7 cut(s) 117, 252, 273, 508, 589, 680, 1107
BseGI GGATG 2 cut(s) 265, 579
BseJI GATNNNNATC 1 cut(s) 918
BseMI GCAATG 1 cut(s) 1555
BseMII CTCAG 5 cut(s) 215, 671, 796, 1187, 1394
BseNI ACTGG 3 cut(s) 229, 852, 1138
BseRI GAGGAG 2 cut(s) 575, 641
BseSI GKGCMC 2 cut(s) 516, 897
BseXI GCAGC 7 cut(s) 108, 122, 141, 144, 414, 1301, 1615
BsgI GTGCAG 1 cut(s) 1377
BshFI GGCC 3 cut(s) 297, 434, 569
BshNI GGYRCC 4 cut(s) 25, 261, 513, 892
BsiHKAI GWGCWC 1 cut(s) 1467
BsiHKCI CYCGRG 1 cut(s) 1543
BsiSI CCGG 3 cut(s) 435, 570, 682
BslFI GGGAC 1 cut(s) 216
BsmAI GTCTC 3 cut(s) 39, 1253, 1276
BsmBI CGTCTC 1 cut(s) 39
BsmFI GGGAC 1 cut(s) 216
BsmI GAATGC 1 cut(s) 156
BsnI GGCC 3 cut(s) 297, 434, 569
BsoBI CYCGRG 1 cut(s) 1543
Bsp119I TTCGAA 1 cut(s) 743
Bsp1286I GDGCHC 3 cut(s) 516, 897, 1467
Bsp143I GATC 9 cut(s) 52, 112, 277, 340, 460, 919, 1030, 1090, 1441
Bsp1720I GCTNAGC 1 cut(s) 1196
Bsp19I CCATGG 1 cut(s) 1107
BspACI CCGC 9 cut(s) 164, 171, 220, 270, 298, 540, 1006, 1133, 1598
BspANI GGCC 3 cut(s) 297, 434, 569
BspCNI CTCAG 5 cut(s) 214, 670, 795, 1188, 1395
BspHI TCATGA 1 cut(s) 979
BspMI ACCTGC 1 cut(s) 1599
BspPI GGATC 5 cut(s) 107, 285, 914, 1025, 1038
BspT104I TTCGAA 1 cut(s) 743
BspT107I GGYRCC 4 cut(s) 25, 261, 513, 892
BsrDI GCAATG 1 cut(s) 1555
BsrI ACTGG 3 cut(s) 229, 852, 1138
BssECI CCNNGG 7 cut(s) 117, 252, 273, 508, 589, 680, 1107
BssMI GATC 9 cut(s) 52, 112, 277, 340, 460, 919, 1030, 1090, 1441
BssT1I CCWWGG 2 cut(s) 117, 1107
Bst2UI CCWGG 5 cut(s) 30, 254, 275, 1155, 1430
Bst4CI ACNGT 5 cut(s) 261, 338, 653, 673, 835
Bst6I CTCTTC 1 cut(s) 1323
BstAPI GCANNNNNTGC 1 cut(s) 135
BstBI TTCGAA 1 cut(s) 743
BstC8I GCNNGC 5 cut(s) 154, 352, 538, 1202, 1596
BstDEI CTNAG 6 cut(s) 201, 657, 782, 1020, 1196, 1403
BstDSI CCRYGG 2 cut(s) 508, 1107
BstEII GGTNACC 1 cut(s) 613
BstF5I GGATG 2 cut(s) 265, 579
BstHHI GCGC 3 cut(s) 35, 604, 1586
BstKTI GATC 9 cut(s) 55, 115, 280, 343, 463, 922, 1033, 1093, 1444
BstMAI GTCTC 3 cut(s) 39, 1253, 1276
BstMBI GATC 9 cut(s) 52, 112, 277, 340, 460, 919, 1030, 1090, 1441
BstMWI GCNNNNNNNGC 2 cut(s) 135, 1201
BstNI CCWGG 5 cut(s) 30, 254, 275, 1155, 1430
BstNSI RCATGY 2 cut(s) 291, 1598
BstPI GGTNACC 1 cut(s) 613
BstSCI CCNGG 7 cut(s) 28, 252, 273, 569, 680, 1153, 1428
BstSFI CTRYAG 4 cut(s) 334, 456, 649, 900
BstSLI GKGCMC 2 cut(s) 516, 897
BstV1I GCAGC 7 cut(s) 108, 122, 141, 144, 414, 1301, 1615
BstV2I GAAGAC 1 cut(s) 1414
BstX2I RGATCY 3 cut(s) 277, 460, 1030
BstYI RGATCY 3 cut(s) 277, 460, 1030
BsuI GTATCC 1 cut(s) 685
BsuRI GGCC 3 cut(s) 297, 434, 569
BtgI CCRYGG 2 cut(s) 508, 1107
BtsCI GGATG 2 cut(s) 265, 579
BtsI GCAGTG 1 cut(s) 1059
BtsIMutI CAGTG 6 cut(s) 222, 343, 790, 859, 1059, 1145
BveI ACCTGC 1 cut(s) 1599
Cac8I GCNNGC 5 cut(s) 154, 352, 538, 1202, 1596
CaiI CAGNNNCTG 1 cut(s) 135
CciI TCATGA 1 cut(s) 979
CfoI GCGC 3 cut(s) 35, 604, 1586
Cfr13I GGNCC 4 cut(s) 176, 230, 502, 567
CseI GACGC 2 cut(s) 56, 743
Csp6I GTAC 5 cut(s) 26, 968, 1017, 1120, 1348
CspCI CAANNNNNGTGG 4 cut(s) 343, 378, 511, 546
CviAII CATG 8 cut(s) 194, 288, 293, 730, 980, 1108, 1262, 1595
CviQI GTAC 5 cut(s) 26, 968, 1017, 1120, 1348
DdeI CTNAG 6 cut(s) 201, 657, 782, 1020, 1196, 1403
DpnI GATC 9 cut(s) 54, 114, 279, 342, 462, 921, 1032, 1092, 1443
DpnII GATC 9 cut(s) 52, 112, 277, 340, 460, 919, 1030, 1090, 1441
DraI TTTAAA 1 cut(s) 315
EaeI YGGCCR 1 cut(s) 295
Eam1104I CTCTTC 1 cut(s) 1323
EarI CTCTTC 1 cut(s) 1323
EciI GGCGGA 1 cut(s) 1122
Eco130I CCWWGG 2 cut(s) 117, 1107
Eco47I GGWCC 3 cut(s) 176, 230, 502
Eco88I CYCGRG 1 cut(s) 1543
Eco91I GGTNACC 1 cut(s) 613
EcoO109I RGGNCCY 2 cut(s) 230, 502
EcoO65I GGTNACC 1 cut(s) 613
EcoRI GAATTC 1 cut(s) 929
EcoRII CCWGG 5 cut(s) 28, 252, 273, 1153, 1428
EcoT14I CCWWGG 2 cut(s) 117, 1107
ErhI CCWWGG 2 cut(s) 117, 1107
Esp3I CGTCTC 1 cut(s) 39
FaeI CATG 8 cut(s) 197, 291, 296, 733, 983, 1111, 1265, 1598
FaqI GGGAC 1 cut(s) 216
FatI CATG 8 cut(s) 193, 287, 292, 729, 979, 1107, 1261, 1594
FauI CCCGC 3 cut(s) 227, 277, 1591
FauNDI CATATG 1 cut(s) 1438
Fnu4HI GCNGC 9 cut(s) 97, 130, 133, 136, 298, 403, 541, 1315, 1629
FokI GGATG 2 cut(s) 252, 586
Fsp4HI GCNGC 9 cut(s) 97, 130, 133, 136, 298, 403, 541, 1315, 1629
FspBI CTAG 3 cut(s) 93, 528, 1445
FspI TGCGCA 1 cut(s) 1585
GlaI GCGC 3 cut(s) 34, 603, 1585
GluI GCNGC 9 cut(s) 97, 130, 133, 136, 298, 403, 541, 1315, 1629
HaeIII GGCC 3 cut(s) 297, 434, 569
HapII CCGG 3 cut(s) 435, 570, 682
HgaI GACGC 2 cut(s) 56, 743
HhaI GCGC 3 cut(s) 35, 604, 1586
Hin1II CATG 8 cut(s) 197, 291, 296, 733, 983, 1111, 1265, 1598
Hin6I GCGC 3 cut(s) 33, 602, 1584
HinP1I GCGC 3 cut(s) 33, 602, 1584
HincII GTYRAC 2 cut(s) 70, 190
HindII GTYRAC 2 cut(s) 70, 190
HindIII AAGCTT 1 cut(s) 1420
HinfI GANTC 6 cut(s) 438, 553, 704, 780, 983, 1367
HpaII CCGG 3 cut(s) 435, 570, 682
HphI GGTGA 5 cut(s) 254, 886, 1094, 1207, 1610
Hpy166II GTNNAC 5 cut(s) 70, 190, 1085, 1120, 1509
Hpy188I TCNGA 5 cut(s) 325, 430, 590, 717, 1293
Hpy188III TCNNGA 8 cut(s) 550, 959, 980, 1168, 1226, 1445, 1499, 1604
Hpy8I GTNNAC 5 cut(s) 70, 190, 1085, 1120, 1509
Hpy99I CGWCG 2 cut(s) 759, 1520
HpyAV CCTTC 3 cut(s) 78, 353, 1046
HpyCH4III ACNGT 5 cut(s) 261, 338, 653, 673, 835
HpyCH4IV ACGT 1 cut(s) 906
HpyF10VI GCNNNNNNNGC 2 cut(s) 135, 1201
HpyF3I CTNAG 6 cut(s) 201, 657, 782, 1020, 1196, 1403
HpySE526I ACGT 1 cut(s) 906
Hsp92II CATG 8 cut(s) 197, 291, 296, 733, 983, 1111, 1265, 1598
HspAI GCGC 3 cut(s) 33, 602, 1584
KflI GGGWCCC 1 cut(s) 230
KpnI GGTACC 1 cut(s) 29
Kzo9I GATC 9 cut(s) 52, 112, 277, 340, 460, 919, 1030, 1090, 1441
LmnI GCTCC 4 cut(s) 88, 326, 1495, 1696
Lsp1109I GCAGC 7 cut(s) 108, 122, 141, 144, 414, 1301, 1615
LweI GCATC 4 cut(s) 755, 787, 1333, 1615
MaeI CTAG 3 cut(s) 93, 528, 1445
MaeII ACGT 1 cut(s) 906
MaeIII GTNAC 3 cut(s) 613, 1125, 1669
MalI GATC 9 cut(s) 54, 114, 279, 342, 462, 921, 1032, 1092, 1443
MboI GATC 9 cut(s) 52, 112, 277, 340, 460, 919, 1030, 1090, 1441
MboII GAAGA 4 cut(s) 643, 785, 1340, 1419
MfeI CAATTG 1 cut(s) 445
MflI RGATCY 3 cut(s) 277, 460, 1030
MhlI GDGCHC 3 cut(s) 516, 897, 1467
MlyI GAGTC 4 cut(s) 447, 698, 774, 977
MmeI TCCRAC 1 cut(s) 61
MseI TTAA 4 cut(s) 306, 314, 1377, 1383
MslI CAYNNNNRTG 2 cut(s) 734, 1623
MspA1I CMGCKG 2 cut(s) 135, 171
MspI CCGG 3 cut(s) 435, 570, 682
MspR9I CCNGG 7 cut(s) 30, 254, 275, 571, 682, 1155, 1430
MunI CAATTG 1 cut(s) 445
Mva1269I GAATGC 1 cut(s) 156
MvaI CCWGG 5 cut(s) 30, 254, 275, 1155, 1430
MwoI GCNNNNNNNGC 2 cut(s) 135, 1201
NciI CCSGG 2 cut(s) 571, 682
NcoI CCATGG 1 cut(s) 1107
NdeI CATATG 1 cut(s) 1438
NdeII GATC 9 cut(s) 52, 112, 277, 340, 460, 919, 1030, 1090, 1441
NlaIII CATG 8 cut(s) 197, 291, 296, 733, 983, 1111, 1265, 1598
NmeAIII GCCGAG 1 cut(s) 209
NmuCI GTSAC 1 cut(s) 1125
NsbI TGCGCA 1 cut(s) 1585
NspI RCATGY 2 cut(s) 291, 1598
NspV TTCGAA 1 cut(s) 743
PaeI GCATGC 1 cut(s) 1598
PaeR7I CTCGAG 1 cut(s) 1543
PagI TCATGA 1 cut(s) 979
PctI GAATGC 1 cut(s) 156
PfeI GAWTC 2 cut(s) 553, 1367
PfoI TCCNGGA 1 cut(s) 1428
PkrI GCNGC 9 cut(s) 98, 131, 134, 137, 299, 404, 542, 1316, 1630
PleI GAGTC 4 cut(s) 446, 698, 774, 977
PpsI GAGTC 4 cut(s) 446, 698, 774, 977
PpuMI RGGWCCY 2 cut(s) 230, 502
Psp5II RGGWCCY 2 cut(s) 230, 502
Psp6I CCWGG 5 cut(s) 28, 252, 273, 1153, 1428
PspEI GGTNACC 1 cut(s) 613
PspGI CCWGG 5 cut(s) 28, 252, 273, 1153, 1428
PspPI GGNCC 4 cut(s) 176, 230, 502, 567
PspPPI RGGWCCY 2 cut(s) 230, 502
PspXI VCTCGAGB 1 cut(s) 1543
PstNI CAGNNNCTG 1 cut(s) 135
PsuI RGATCY 3 cut(s) 277, 460, 1030
PvuII CAGCTG 1 cut(s) 135
RsaI GTAC 5 cut(s) 27, 969, 1018, 1121, 1349
RsaNI GTAC 5 cut(s) 26, 968, 1017, 1120, 1348
RseI CAYNNNNRTG 2 cut(s) 734, 1623
SaqAI TTAA 4 cut(s) 306, 314, 1377, 1383
SatI GCNGC 9 cut(s) 97, 130, 133, 136, 298, 403, 541, 1315, 1629
Sau3AI GATC 9 cut(s) 52, 112, 277, 340, 460, 919, 1030, 1090, 1441
Sau96I GGNCC 4 cut(s) 176, 230, 502, 567
SchI GAGTC 4 cut(s) 447, 698, 774, 977
ScrFI CCNGG 7 cut(s) 30, 254, 275, 571, 682, 1155, 1430
SduI GDGCHC 3 cut(s) 516, 897, 1467
SfaNI GCATC 4 cut(s) 755, 787, 1333, 1615
SfcI CTRYAG 4 cut(s) 334, 456, 649, 900
Sfr274I CTCGAG 1 cut(s) 1543
SfuI TTCGAA 1 cut(s) 743
SinI GGWCC 3 cut(s) 176, 230, 502
SlaI CTCGAG 1 cut(s) 1543
SmiMI CAYNNNNRTG 2 cut(s) 734, 1623
SmlI CTYRAG 2 cut(s) 1250, 1543
SmoI CTYRAG 2 cut(s) 1250, 1543
SpeI ACTAGT 1 cut(s) 527
SphI GCATGC 1 cut(s) 1598
SsiI CCGC 9 cut(s) 164, 171, 220, 270, 298, 540, 1006, 1133, 1598
SspMI CTAG 3 cut(s) 93, 528, 1445
StyD4I CCNGG 7 cut(s) 28, 252, 273, 569, 680, 1153, 1428
StyI CCWWGG 2 cut(s) 117, 1107
TaaI ACNGT 5 cut(s) 261, 338, 653, 673, 835
TaiI ACGT 1 cut(s) 909
TatI WGTACW 3 cut(s) 967, 1016, 1119
TauI GCSGC 2 cut(s) 300, 543
TfiI GAWTC 2 cut(s) 553, 1367
Tru1I TTAA 4 cut(s) 306, 314, 1377, 1383
Tru9I TTAA 4 cut(s) 306, 314, 1377, 1383
TscAI CASTG 6 cut(s) 229, 343, 790, 859, 1066, 1145
TseFI GTSAC 1 cut(s) 1125
TseI GCWGC 7 cut(s) 96, 129, 132, 135, 402, 1314, 1628
Tsp45I GTSAC 1 cut(s) 1125
TspDTI ATGAA 4 cut(s) 17, 746, 986, 1187
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 6 cut(s) 229, 343, 790, 859, 1066, 1145
VpaK11BI GGWCC 3 cut(s) 176, 230, 502
XapI RAATTY 4 cut(s) 122, 310, 929, 1659
XbaI TCTAGA 1 cut(s) 1444
XceI RCATGY 2 cut(s) 291, 1598
XhoI CTCGAG 1 cut(s) 1543
XspI CTAG 3 cut(s) 93, 528, 1445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.