RchiOBHm_Chr5g0028241

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000079
Physical Location & Seq
Reverse (-)
22088791 .. 22094758
5968 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30769

Sequence Viewer

Length: 2481 bp
ATGCACTTCTTTCTCACGTACTCTCTTTCCTGCCAACACTGTGGTGCTGTGAGGACCAGCATTTTGTCTGCAAGATGGAGGAACATGTGGACTACTCTTCCCAATCTAGACTTTGATAACGAAAGAGATTCATTTGGTGTCACGATAGGTCCCTATGGCAAGGATCGTTTCACGACGTTTGTTGATCGTGTATTTCTATACCGTGATTCATCTGACATTAAAAAGCTTGGTCTTAAAATCCGTTATCCTGAAGATTTTTCTCGTATCGATGATTGGATCTGCACAGCCGTTAGGCGTAATGTTTTGGAAGTTGATCTTCATGTCTTCTCTGGTGAAATTGAAGAAGAAGTTGGTTTTCAGTTCCCTCAGAGCCTTTTTATGTGCAAAACTCTGAAGGTTTTGAAATTGTATTCAAATTCATGGACTCTTAAGTATCCTCCTCCTCCGCCAGGGTGTTTTCCAAATCTTAAGTTCCTCCATGTTGAATTTGATTTCCTCGATCCGGACACATTGAAAAATCTGTTTTCTCGCTGCCCTATGCTTAAGGAGTTGTCTGTACATGGATTCATTGACTGTGAATATGCTAGGGATTATGATATCTACAAATGTGCGCCTGAATTGAACATGTTGAGAATAGAAGTGTGTGATCCTTATCAGTATTGCTGTTTTTTCACTAATACCCCAAAGCTTGAATACATTGATATTAAATCAGCAGCTCTATCAAGTTATTTCTTAATGAATTCAAACGGCCTAGTCAATGCCAGTATTGTTTTGGAAGAACGTTGTTCTAATAATAATGATAAATACCAACTGTGCCGGTTAGCTGAGTTTTTGTCACAAACAACAAATGTCAATCATCTTTCTCTTGAAATCCATGATTATTGTCTTCAGGCTTGGTATGATTTTGGTAATGTGAACCAAATGAAGCTGGTACTTCATGATTGCTATTACTCGGAAGTACTAGCAGTGCTGCTCACAAAATCACCTAAGCTTGTAGATCTTGCCTTGGAACATGTTGAGATGGATGTAGCACAGTACATGTTAAAGAATGGTCACGTTTTGAATAACATGACAATCTATACAGGCCATCTGTGTAAGAAAGAGAAGGAGTTATACAAGAAATTTGTGATGTTTGAAAGGCGTTCAATGACTTGTTGTGGAAGGAACATGAACTTTTTGGTTGAGGTTTCAATGTCAAAGAGTCAAGCAAGAGTAAAAGATAGGATTAGTGCATTACCTGACGAAATTCTTTGCAAAATACTTTCTTGCCTTGAACTACAATGTGCTGTGAGGACCACCGTTTTGTCCCCCAGATGGAAGAATTTATGGACTTCTCTTCCCAACCTACGGTTCTGCAATGAAGATTTTAGTGATGATGATGCTTTTATGACGTTTGTGGATCGTGTTCTTTTCTTTCGTGACTCGTCAGACATCAAAAAATTCTCTCTTGACCTCGAGAAACCAAAGGATGATTTCTCTCGTATTGAAAGTTGGATTTGTACCGCCATTAGGCGGAATGTTGTTGAACTTTATCTTTGTGTCAATTTATATCCGGACAAGACTTTTGAATTGCCTCGAAGCCTTTTCACTTGCAAAACACTAGAGGTGTTGTACCTGACGTCAAATTTTATTGCCAATCCTCCAAAATCTGGGTGTTTCCCGAATCTCAAGTTCCTCGATGTTCTGGTTCATTTTCCTAACCATAATAACTCGTCATTTGGACAGATTTTCTCTAACTGCCCTGTGCTTGAGGATTTGAGAATTGATGGGCCTCTTGGGGCGTATGATGAAGTTTTGGATTTCAACATCTCTGCACCTGAACTGAAGAGATTAACAATAGGATTGTACATTGACGATTTTGAGGATGAAGAGTATAATTTTCATATTAATGCTCCAAAACTTGAAGTCTTGAACCTCCCTGAGGGTGATTCGTCAGTGTTTTCATTTGAGATCAGTGCAAAGTCCCTTGTCAAAGCCAATATCAATCTTTCTAGCTGTCGTGTACATTTTGACAGTACAAATGGACTTCTGGAAGCCATCTCCAATGTCAAACAGCTGTCTCTTTCAAATCATTATTTGGAGGGTGGTGTACCTGCTTTTGATAATCTGAACCATTTGGAACTGTCTCTTTGTGGTTTCACCTGCTGGCGATATCTAATAGAGTTTCTCAAGAGATCACGGAATCTAGAATATCTTGTTATCAAACATCATTGCAAAGGTCAGCATGTGGACTTTACTCCTCCAGCGTTTGTGCCTGTTTGTATGTTGTCACATCTCAAGACTATCACAATAGCAAGCTTTGAGGGAAGGCAGGATGAAATGAGAGTTGCTCAGTATGTGCTAAAGTACAGTGAAGTTTTAAATAAGATGACTATTTTTACCATTTCAGGTCTTCCAAGAGCAGAAGAGGAGGCATTGTACAAAGAATTTTTGATGTTCGAACGGGGTTCAAAGACTTGTCAAGTTGAAATATGTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

826

Amino Acids

95.67

Weight (kDa)

5.74

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 71 - 343 4.1e-08 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 90 - 188 3.8e-09 FBD-associated F-box protein At5g56370, LRR repeats
F-box PF00646 409 - 449 8.8e-07 F-box domain
LRR_At1g61320_AtMIF1 PF23622 475 - 806 1.8e-13 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 496 - 639 7.1e-12 FBD-associated F-box protein At5g56370, LRR repeats
FBD PF08387 748 - 792 1.6e-13 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000079)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32020
fragaria_vesca FvH4_2g37070 FvH4_3g17062 FvH4_3g17580 FvH4_3g17580 FvH4_3g17580 FvH4_3g17581 FvH4_3g18162 FvH4_3g20210 FvH4_3g32940 FvH4_3g33370 FvH4_3g33390 FvH4_3g33400 FvH4_3g33400 FvH4_3g33400 FvH4_3g33401 FvH4_3g33430 FvH4_4g00640 FvH4_5g02210 FvH4_5g02220 FvH4_5g02220 FvH4_5g02221 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02230 FvH4_5g02280 FvH4_5g02290 FvH4_5g02290 FvH4_5g08740 FvH4_5g24470 FvH4_6g07500 FvH4_6g07500 FvH4_6g07500 FvH4_6g07500 FvH4_6g08520 FvH4_6g10000 FvH4_7g28000 FvH4_7g28011 FvH4_7g28030 FvH4_7g28040 FvH4_7g28042
malus_domestica MD03G1112000.v1.1 MD03G1112100.v1.1 MD03G1112200.v1.1 MD03G1253000.v1.1 MD03G1253100.v1.1 MD03G1253400.v1.1 MD03G1255400.v1.1 MD03G1276300.v1.1 MD03G1276400.v1.1 MD05G1193700.v1.1 MD11G1265000.v1.1 MD11G1265100.v1.1 MD11G1265300.v1.1 MD11G1275400.v1.1 MD11G1275600.v1.1
prunus_persica Prupe.4G151700_v2.0.a1 Prupe.4G160800_v2.0.a1 Prupe.4G160900_v2.0.a1 Prupe.4G161000_v2.0.a1 Prupe.4G161100_v2.0.a1 Prupe.4G161200_v2.0.a1 Prupe.4G161300_v2.0.a1 Prupe.5G021800_v2.0.a1
pyrus_communis pycom03g06140 pycom03g19340 pycom03g20150 pycom03g20210 pycom05g18110 pycom11g23320 pycom11g24360 pycom11g24380
rosa_chinensis RchiOBHm_Chr1g0321391 RchiOBHm_Chr1g0363171 RchiOBHm_Chr1g0363181 RchiOBHm_Chr2g0115721 RchiOBHm_Chr3g0458571 RchiOBHm_Chr3g0460681 RchiOBHm_Chr4g0387591 RchiOBHm_Chr5g0028191 RchiOBHm_Chr5g0028201 RchiOBHm_Chr5g0028241 RchiOBHm_Chr5g0028261 RchiOBHm_Chr5g0028411 RchiOBHm_Chr5g0028431 RchiOBHm_Chr5g0029291 RchiOBHm_Chr5g0030601 RchiOBHm_Chr5g0030631 RchiOBHm_Chr5g0059941 RchiOBHm_Chr5g0059951 RchiOBHm_Chr5g0059961 RchiOBHm_Chr5g0060061 RchiOBHm_Chr5g0060761 RchiOBHm_Chr5g0060771 RchiOBHm_Chr6g0304711 RchiOBHm_Chr7g0192751 RchiOBHm_Chr7g0197861 RchiOBHm_Chr7g0197881 RchiOBHm_Chr7g0197891 RchiOBHm_Chr7g0197921
rosa_laevigata RLG00000003903 RLG00000003904 RLG00000004332 RLG00000010109 RLG00000010957 RLG00000018203 RLG00000024989 RLG00000025147 RLG00000027586 RLG00000030426 RLG00000033074 RLG00000033075 RLG00000033076 RLG00000033094 RLG00000033150 RLG00000033234 RLG00000035345 RLG00000035399
rosa_multiflora Rmu_co8271835.1_g000001 Rmu_co8353127.1_g000001 Rmu_co8434667.1_g000001 Rmu_co8434713.1_g000001 Rmu_co8441243.1_g000001 Rmu_co8501607.1_g000001 Rmu_sc0000471.1_g000010 Rmu_sc0000724.1_g000008 Rmu_sc0000727.1_g000033 Rmu_sc0000814.1_g000032 Rmu_sc0000960.1_g000003 Rmu_sc0001082.1_g000014 Rmu_sc0001082.1_g000016 Rmu_sc0001571.1_g000005 Rmu_sc0001571.1_g000007 Rmu_sc0001571.1_g000008 Rmu_sc0001571.1_g000020 Rmu_sc0002040.1_g000017 Rmu_sc0002925.1_g000003 Rmu_sc0003864.1_g000006 Rmu_sc0005482.1_g000001 Rmu_sc0005482.1_g000005 Rmu_sc0006771.1_g000006 Rmu_sc0007143.1_g000008 Rmu_sc0007248.1_g000006 Rmu_sc0011593.1_g000004 Rmu_sc0011593.1_g000006 Rmu_sc0011593.1_g000007 Rmu_sc0011593.1_g000012 Rmu_sc0016104.1_g000007 Rmu_sc0022819.1_g000001 Rmu_sc0025685.1_g000002 Rmu_sc0027256.1_g000002 Rmu_sc0034563.1_g000001 Rmu_sc0034642.1_g000001 Rmu_ssc0000197.1_g000059
rosa_roxburghii Rroxscaffold_1G00019950 Rroxscaffold_1G00020580 Rroxscaffold_1G00051250 Rroxscaffold_1G00051270 Rroxscaffold_1G00051470 Rroxscaffold_1G00051480 Rroxscaffold_2G00127930 Rroxscaffold_2G00128790 Rroxscaffold_3G00258110 Rroxscaffold_3G00258130 Rroxscaffold_6G00419310 Rroxscaffold_6G00420970 Rroxscaffold_6G00421360 Rroxscaffold_7G00163570
rosa_rugosa Rorug01G0032500 Rorug02G0199500.1 Rorug02G0199600.1 Rorug03G0024200 Rorug03G0029800 Rorug03G0029900 Rorug03G0045000 Rorug03G0045000 Rorug03G0310900 Rorug05G0104800 Rorug05G0104900 Rorug05G0105000 Rorug05G0105100 Rorug05G0105200 Rorug05G0105300 Rorug05G0107400 Rorug05G0107600 Rorug05G0112900 Rorug05G0118900 Rorug05G0327000 Rorug05G0327000 Rorug05G0327000 Rorug05G0328000 Rorug05G0333900 Rorug06G0333700 Rorug07G0004400 Rorug07G0044100 Rorug07G0044200 Rorug07G0044200 Rorug07G0044300 Rorug07G0044400
rosa_samantha Rh1AG314000 Rh1BG277500 Rh1CG293900 Rh1DG051800 Rh1DG308100 Rh1DG308200 Rh2AG256700 Rh2AG295900 Rh2BG267300 Rh2CG262300 Rh2DG265100 Rh2DG319900 Rh3AG084700 Rh3AG088100 Rh3AG102600 Rh3BG087500 Rh3CG088000 Rh3CG091300 Rh3CG108200 Rh3DG088400 Rh4AG017000 Rh4CG018100 Rh4DG012700 Rh5AG198100 Rh5AG198200 Rh5AG198300 Rh5AG198500 Rh5AG198600 Rh5AG198700 Rh5AG200400 Rh5AG205000 Rh5AG211100 Rh5AG244900 Rh5AG391100 Rh5AG391200 Rh5AG396500 Rh5BG197000 Rh5BG197100 Rh5BG197200 Rh5BG197300 Rh5BG198600 Rh5BG198800 Rh5BG403400 Rh5BG403500 Rh5CG216700 Rh5CG216900 Rh5CG217000 Rh5CG218500 Rh5CG225000 Rh5CG427400 Rh5CG427500 Rh5CG433300 Rh5DG198400 Rh5DG198500 Rh5DG198800 Rh5DG198900 Rh5DG199000 Rh5DG200700 Rh5DG201000 Rh5DG207200 Rh5DG215000 Rh5DG374300 Rh5DG419000 Rh5DG419100 Rh5DG420200 Rh5DG423900 Rh6BG456100 Rh6CG458700 Rh7AG129400 Rh7AG168100 Rh7AG457700 Rh7BG170900 Rh7CG177400 Rh7CG177500 Rh7DG131600 Rh7DG169800 Rh7DG169900 Rh7DG170000 Rh7DG170100
rosa_wichuraiana Rw1G027820 Rw2G020090 Rw3G007530 Rw3G008730 Rw4G001080 Rw5G018080 Rw5G018090 Rw5G018100 Rw5G018110 Rw5G018120 Rw5G018640 Rw5G019310 Rw5G036810 Rw5G036820 Rw5G036830 Rw5G037390 Rw6G038770 Rw7G011070 Rw7G014650 Rw7G014660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2150
AatII GACGTC 1 cut(s) 1622
Acc36I ACCTGC 2 cut(s) 2101, 2150
AccB7I CCANNNNNTGG 1 cut(s) 1649
AccIII TCCGGA 2 cut(s) 502, 1552
AciI CCGC 3 cut(s) 446, 1503, 1513
AclI AACGTT 1 cut(s) 781
AclWI GGATC 5 cut(s) 171, 284, 494, 641, 1407
AcsI RAATTY 9 cut(s) 415, 485, 739, 1121, 1245, 1321, 1439, 1624, 2426
AcuI CTGAAG 4 cut(s) 270, 413, 872, 1844
AcyI GRCGYC 1 cut(s) 1619
AfiI CCNNNNNNNGG 8 cut(s) 449, 502, 1314, 1347, 1509, 1512, 1649, 1921
AflII CTTAAG 3 cut(s) 428, 467, 542
AflIII ACRYGT 4 cut(s) 84, 624, 1012, 1038
AjnI CCWGG 1 cut(s) 448
AjuI GAANNNNNNNTTGG 4 cut(s) 333, 365, 1334, 1366
AleI CACNNNNGTG 1 cut(s) 42
AluBI AGCT 9 cut(s) 226, 688, 716, 824, 928, 991, 1995, 2056, 2298
AluI AGCT 9 cut(s) 226, 688, 716, 824, 928, 991, 1995, 2056, 2298
Alw26I GTCTC 2 cut(s) 2064, 2130
AlwI GGATC 5 cut(s) 171, 284, 494, 641, 1407
Ama87I CYCGRG 1 cut(s) 1454
Aor13HI TCCGGA 2 cut(s) 502, 1552
AoxI GGCC 3 cut(s) 748, 1084, 1769
ApeKI GCWGC 3 cut(s) 531, 713, 970
ApoI RAATTY 9 cut(s) 415, 485, 739, 1121, 1245, 1321, 1439, 1624, 2426
ArsI GACNNNNNNTTYG 6 cut(s) 46, 78, 1285, 1317, 1547, 1579
AseI ATTAAT 1 cut(s) 1887
AspLEI GCGC 1 cut(s) 613
AspS9I GGNCC 4 cut(s) 54, 149, 1293, 1769
AsuHPI GGTGA 4 cut(s) 344, 975, 1937, 2131
AsuII TTCGAA 1 cut(s) 2439
AvaI CYCGRG 1 cut(s) 1454
AvaII GGWCC 3 cut(s) 54, 149, 1293
AxyI CCTNAGG 1 cut(s) 1920
BarI GAAGNNNNNNTAC 2 cut(s) 1097, 1129
BbsI GAAGAC 3 cut(s) 316, 878, 2384
BbvI GCAGC 3 cut(s) 518, 725, 957
BccI CCATC 6 cut(s) 69, 1015, 1095, 1308, 1760, 2045
BceAI ACGGC 2 cut(s) 272, 763
BciT130I CCWGG 1 cut(s) 450
BciVI GTATCC 1 cut(s) 444
BcoDI GTCTC 2 cut(s) 2064, 2130
BfaI CTAG 8 cut(s) 107, 585, 752, 962, 1601, 1992, 2186, 2479
BfrI CTTAAG 3 cut(s) 428, 467, 542
BfuAI ACCTGC 2 cut(s) 2101, 2150
BfuI GTATCC 1 cut(s) 444
BglII AGATCT 1 cut(s) 997
BisI GCNGC 3 cut(s) 532, 714, 971
BlsI GCNGC 3 cut(s) 533, 715, 972
BmcAI AGTACT 1 cut(s) 960
Bme1390I CCNGG 1 cut(s) 450
Bme18I GGWCC 3 cut(s) 54, 149, 1293
BmeT110I CYCGRG 1 cut(s) 1454
BmgT120I GGNCC 4 cut(s) 54, 149, 1293, 1769
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 1 cut(s) 450
BmsI GCATC 1 cut(s) 1369
BpiI GAAGAC 3 cut(s) 316, 878, 2384
BplI GAGNNNNNCTC 2 cut(s) 2314, 2346
BpmI CTGGAG 1 cut(s) 2226
Bpu10I CCTNAGC 1 cut(s) 987
Bpu14I TTCGAA 1 cut(s) 2439
BpuEI CTTGAG 4 cut(s) 1652, 1769, 2153, 2261
Bsa29I ATCGAT 1 cut(s) 267
BsaAI YACGTR 1 cut(s) 18
BsaBI GATNNNNATC 1 cut(s) 651
BsaHI GRCGYC 1 cut(s) 1619
BsaJI CCNNGG 2 cut(s) 449, 1005
BsaWI WCCGGW 2 cut(s) 502, 1552
BsaXI ACNNNNNCTCC 2 cut(s) 2402, 2432
Bsc4I CCNNNNNNNGG 8 cut(s) 449, 502, 1314, 1347, 1509, 1512, 1649, 1921
Bse118I RCCGGY 1 cut(s) 816
Bse1I ACTGG 1 cut(s) 762
Bse21I CCTNAGG 1 cut(s) 1920
Bse3DI GCAATG 2 cut(s) 1363, 2209
Bse8I GATNNNNATC 1 cut(s) 651
BseAI TCCGGA 2 cut(s) 502, 1552
BseBI CCWGG 1 cut(s) 450
BseCI ATCGAT 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 449, 1005
BseGI GGATG 4 cut(s) 1030, 1474, 1870, 2320
BseJI GATNNNNATC 1 cut(s) 651
BseLI CCNNNNNNNGG 8 cut(s) 449, 502, 1314, 1347, 1509, 1512, 1649, 1921
BseMI GCAATG 2 cut(s) 1363, 2209
BseMII CTCAG 4 cut(s) 380, 816, 1911, 2345
BseNI ACTGG 1 cut(s) 762
BseRI GAGGAG 4 cut(s) 429, 432, 2229, 2423
BseXI GCAGC 3 cut(s) 518, 725, 957
BsgI GTGCAG 2 cut(s) 265, 1797
BshFI GGCC 3 cut(s) 750, 1086, 1771
BshVI ATCGAT 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 1454
BsiSI CCGG 3 cut(s) 503, 817, 1553
BslFI GGGAC 3 cut(s) 135, 1291, 1948
BslI CCNNNNNNNGG 8 cut(s) 449, 502, 1314, 1347, 1509, 1512, 1649, 1921
BsmAI GTCTC 2 cut(s) 2064, 2130
BsmFI GGGAC 3 cut(s) 135, 1291, 1948
BsnI GGCC 3 cut(s) 750, 1086, 1771
BsoBI CYCGRG 1 cut(s) 1454
Bsp119I TTCGAA 1 cut(s) 2439
Bsp13I TCCGGA 2 cut(s) 502, 1552
Bsp1407I TGTACA 4 cut(s) 556, 1845, 2002, 2418
BspACI CCGC 3 cut(s) 446, 1503, 1513
BspANI GGCC 3 cut(s) 750, 1086, 1771
BspCNI CTCAG 4 cut(s) 379, 817, 1912, 2344
BspDI ATCGAT 1 cut(s) 267
BspEI TCCGGA 2 cut(s) 502, 1552
BspHI TCATGA 1 cut(s) 937
BspLI GGNNCC 1 cut(s) 151
BspMI ACCTGC 2 cut(s) 2101, 2150
BspPI GGATC 5 cut(s) 171, 284, 494, 641, 1407
BspT104I TTCGAA 1 cut(s) 2439
BspTI CTTAAG 3 cut(s) 428, 467, 542
BsrDI GCAATG 2 cut(s) 1363, 2209
BsrFI RCCGGY 1 cut(s) 816
BsrGI TGTACA 4 cut(s) 556, 1845, 2002, 2418
BsrI ACTGG 1 cut(s) 762
BssAI RCCGGY 1 cut(s) 816
BssECI CCNNGG 2 cut(s) 449, 1005
BssNI GRCGYC 1 cut(s) 1619
BssT1I CCWWGG 1 cut(s) 1005
Bst2UI CCWGG 1 cut(s) 450
Bst6I CTCTTC 5 cut(s) 102, 1341, 1820, 1863, 2400
BstACI GRCGYC 1 cut(s) 1619
BstAFI CTTAAG 3 cut(s) 428, 467, 542
BstAUI TGTACA 4 cut(s) 556, 1845, 2002, 2418
BstBAI YACGTR 1 cut(s) 18
BstBI TTCGAA 1 cut(s) 2439
BstC8I GCNNGC 2 cut(s) 2147, 2296
BstDEI CTNAG 5 cut(s) 366, 825, 987, 1920, 2331
BstENI CCTNNNNNAGG 2 cut(s) 447, 1919
BstF5I GGATG 4 cut(s) 1030, 1474, 1870, 2320
BstHHI GCGC 1 cut(s) 613
BstMAI GTCTC 2 cut(s) 2064, 2130
BstNI CCWGG 1 cut(s) 450
BstNSI RCATGY 5 cut(s) 88, 628, 1016, 1042, 2228
BstSCI CCNGG 1 cut(s) 448
BstV1I GCAGC 3 cut(s) 518, 725, 957
BstV2I GAAGAC 3 cut(s) 316, 878, 2384
BstX2I RGATCY 2 cut(s) 276, 997
BstXI CCANNNNNNTGG 1 cut(s) 41
BstYI RGATCY 2 cut(s) 276, 997
Bsu15I ATCGAT 1 cut(s) 267
Bsu36I CCTNAGG 1 cut(s) 1920
BsuI GTATCC 1 cut(s) 444
BsuRI GGCC 3 cut(s) 750, 1086, 1771
BsuTUI ATCGAT 1 cut(s) 267
BtsCI GGATG 4 cut(s) 1030, 1474, 1870, 2320
BtsI GCAGTG 1 cut(s) 972
BtsIMutI CAGTG 5 cut(s) 37, 972, 1941, 1960, 2356
BveI ACCTGC 2 cut(s) 2101, 2150
Cac8I GCNNGC 2 cut(s) 2147, 2296
CciI TCATGA 1 cut(s) 937
CfoI GCGC 1 cut(s) 613
Cfr10I RCCGGY 1 cut(s) 816
Cfr13I GGNCC 4 cut(s) 54, 149, 1293, 1769
ClaI ATCGAT 1 cut(s) 267
DdeI CTNAG 5 cut(s) 366, 825, 987, 1920, 2331
DraI TTTAAA 1 cut(s) 2361
Eam1104I CTCTTC 5 cut(s) 102, 1341, 1820, 1863, 2400
EarI CTCTTC 5 cut(s) 102, 1341, 1820, 1863, 2400
EciI GGCGGA 2 cut(s) 435, 1528
Eco130I CCWWGG 1 cut(s) 1005
Eco32I GATATC 2 cut(s) 598, 2153
Eco47I GGWCC 3 cut(s) 54, 149, 1293
Eco57I CTGAAG 4 cut(s) 270, 413, 872, 1844
Eco81I CCTNAGG 1 cut(s) 1920
Eco88I CYCGRG 1 cut(s) 1454
EcoNI CCTNNNNNAGG 2 cut(s) 447, 1919
EcoO109I RGGNCCY 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 739
EcoRII CCWGG 1 cut(s) 448
EcoRV GATATC 2 cut(s) 598, 2153
EcoT14I CCWWGG 1 cut(s) 1005
ErhI CCWWGG 1 cut(s) 1005
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FaqI GGGAC 3 cut(s) 135, 1291, 1948
Fnu4HI GCNGC 3 cut(s) 532, 714, 971
FokI GGATG 4 cut(s) 1037, 1481, 1877, 2327
Fsp4HI GCNGC 3 cut(s) 532, 714, 971
FspBI CTAG 8 cut(s) 107, 585, 752, 962, 1601, 1992, 2186, 2479
GlaI GCGC 1 cut(s) 612
GluI GCNGC 3 cut(s) 532, 714, 971
GsuI CTGGAG 1 cut(s) 2226
HaeIII GGCC 3 cut(s) 750, 1086, 1771
HapII CCGG 3 cut(s) 503, 817, 1553
HhaI GCGC 1 cut(s) 613
Hin1I GRCGYC 1 cut(s) 1619
Hin6I GCGC 1 cut(s) 611
HinP1I GCGC 1 cut(s) 611
HindIII AAGCTT 4 cut(s) 224, 686, 989, 2296
HinfI GANTC 9 cut(s) 128, 206, 424, 564, 1201, 1421, 1663, 1928, 2182
HpaII CCGG 3 cut(s) 503, 817, 1553
HphI GGTGA 4 cut(s) 344, 975, 1937, 2131
Hpy166II GTNNAC 5 cut(s) 90, 916, 2003, 2090, 2230
Hpy188I TCNGA 6 cut(s) 214, 369, 393, 955, 1429, 2109
Hpy8I GTNNAC 5 cut(s) 90, 916, 2003, 2090, 2230
Hpy99I CGWCG 1 cut(s) 178
HpyAV CCTTC 4 cut(s) 388, 1099, 1155, 2301
HpyCH4IV ACGT 6 cut(s) 17, 176, 781, 1056, 1391, 1619
HpyF3I CTNAG 5 cut(s) 366, 825, 987, 1920, 2331
HpySE526I ACGT 6 cut(s) 17, 176, 781, 1056, 1391, 1619
Hsp92I GRCGYC 1 cut(s) 1619
HspAI GCGC 1 cut(s) 611
Kpn2I TCCGGA 2 cut(s) 502, 1552
LmnI GCTCC 1 cut(s) 1897
Lsp1109I GCAGC 3 cut(s) 518, 725, 957
LweI GCATC 1 cut(s) 1369
MaeI CTAG 8 cut(s) 107, 585, 752, 962, 1601, 1992, 2186, 2479
MaeII ACGT 6 cut(s) 17, 176, 781, 1056, 1391, 1619
MaeIII GTNAC 6 cut(s) 139, 834, 1052, 1418, 2268, 2474
MflI RGATCY 2 cut(s) 276, 997
MlyI GAGTC 3 cut(s) 418, 1210, 1415
MmeI TCCRAC 1 cut(s) 1472
MroI TCCGGA 2 cut(s) 502, 1552
MslI CAYNNNNRTG 2 cut(s) 42, 1887
MspA1I CMGCKG 1 cut(s) 2056
MspCI CTTAAG 3 cut(s) 428, 467, 542
MspI CCGG 3 cut(s) 503, 817, 1553
MspR9I CCNGG 1 cut(s) 450
MvaI CCWGG 1 cut(s) 450
NlaIV GGNNCC 1 cut(s) 151
NmuCI GTSAC 5 cut(s) 139, 834, 1052, 1418, 2268
NspI RCATGY 5 cut(s) 88, 628, 1016, 1042, 2228
NspV TTCGAA 1 cut(s) 2439
OliI CACNNNNGTG 1 cut(s) 42
PaeR7I CTCGAG 1 cut(s) 1454
PagI TCATGA 1 cut(s) 937
PaqCI CACCTGC 1 cut(s) 2150
PciI ACATGT 4 cut(s) 84, 624, 1012, 1038
PfeI GAWTC 6 cut(s) 128, 206, 564, 1663, 1928, 2182
PflMI CCANNNNNTGG 1 cut(s) 1649
PkrI GCNGC 3 cut(s) 533, 715, 972
PleI GAGTC 3 cut(s) 418, 1209, 1415
PpsI GAGTC 3 cut(s) 418, 1209, 1415
Ppu21I YACGTR 1 cut(s) 18
PpuMI RGGWCCY 1 cut(s) 149
PscI ACATGT 4 cut(s) 84, 624, 1012, 1038
PshBI ATTAAT 1 cut(s) 1887
Psp1406I AACGTT 1 cut(s) 781
Psp5II RGGWCCY 1 cut(s) 149
Psp6I CCWGG 1 cut(s) 448
PspGI CCWGG 1 cut(s) 448
PspN4I GGNNCC 1 cut(s) 151
PspPI GGNCC 4 cut(s) 54, 149, 1293, 1769
PspPPI RGGWCCY 1 cut(s) 149
PsuI RGATCY 2 cut(s) 276, 997
PvuII CAGCTG 1 cut(s) 2056
RseI CAYNNNNRTG 2 cut(s) 42, 1887
SatI GCNGC 3 cut(s) 532, 714, 971
Sau96I GGNCC 4 cut(s) 54, 149, 1293, 1769
ScaI AGTACT 1 cut(s) 960
SchI GAGTC 3 cut(s) 418, 1210, 1415
ScrFI CCNGG 1 cut(s) 450
SfaNI GCATC 1 cut(s) 1369
Sfr274I CTCGAG 1 cut(s) 1454
SfuI TTCGAA 1 cut(s) 2439
SinI GGWCC 3 cut(s) 54, 149, 1293
SlaI CTCGAG 1 cut(s) 1454
SmiMI CAYNNNNRTG 2 cut(s) 42, 1887
SmlI CTYRAG 8 cut(s) 428, 467, 542, 1454, 1667, 1748, 2168, 2276
SmoI CTYRAG 8 cut(s) 428, 467, 542, 1454, 1667, 1748, 2168, 2276
SsiI CCGC 3 cut(s) 446, 1503, 1513
SspMI CTAG 8 cut(s) 107, 585, 752, 962, 1601, 1992, 2186, 2479
StyD4I CCNGG 1 cut(s) 448
StyI CCWWGG 1 cut(s) 1005
TaiI ACGT 6 cut(s) 20, 179, 784, 1059, 1394, 1622
TaqI TCGA 6 cut(s) 267, 498, 1455, 1576, 1677, 2439
TatI WGTACW 8 cut(s) 556, 958, 1035, 1845, 2002, 2015, 2346, 2418
TfiI GAWTC 6 cut(s) 128, 206, 564, 1663, 1928, 2182
TscAI CASTG 5 cut(s) 44, 972, 1941, 1960, 2356
TseFI GTSAC 5 cut(s) 139, 834, 1052, 1418, 2268
TseI GCWGC 3 cut(s) 531, 713, 970
Tsp45I GTSAC 5 cut(s) 139, 834, 1052, 1418, 2268
TspGWI ACGGA 2 cut(s) 230, 2194
TspRI CASTG 5 cut(s) 44, 972, 1941, 1960, 2356
Van91I CCANNNNNTGG 1 cut(s) 1649
Vha464I CTTAAG 3 cut(s) 428, 467, 542
VpaK11BI GGWCC 3 cut(s) 54, 149, 1293
VspI ATTAAT 1 cut(s) 1887
XagI CCTNNNNNAGG 2 cut(s) 447, 1919
XapI RAATTY 9 cut(s) 415, 485, 739, 1121, 1245, 1321, 1439, 1624, 2426
XbaI TCTAGA 2 cut(s) 106, 2185
XceI RCATGY 5 cut(s) 88, 628, 1016, 1042, 2228
XcmI CCANNNNNNNNNTGG 1 cut(s) 769
XhoI CTCGAG 1 cut(s) 1454
XspI CTAG 8 cut(s) 107, 585, 752, 962, 1601, 1992, 2186, 2479
ZraI GACGTC 1 cut(s) 1620
ZrmI AGTACT 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.