peroxisome matrix targeting signal-2 binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 675 |
| AccI | GTMKAC | 1 cut(s) | 1166 |
| AccII | CGCG | 2 cut(s) | 27, 215 |
| AciI | CCGC | 4 cut(s) | 215, 245, 884, 1081 |
| AclI | AACGTT | 1 cut(s) | 181 |
| AclWI | GGATC | 3 cut(s) | 44, 875, 888 |
| AcsI | RAATTY | 4 cut(s) | 299, 383, 685, 814 |
| AcuI | CTGAAG | 2 cut(s) | 578, 1191 |
| AfaI | GTAC | 3 cut(s) | 192, 586, 1187 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 395, 946 |
| AflIII | ACRYGT | 2 cut(s) | 135, 284 |
| AjnI | CCWGG | 2 cut(s) | 774, 1016 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 401, 433 |
| AluBI | AGCT | 4 cut(s) | 643, 925, 1000, 1175 |
| AluI | AGCT | 4 cut(s) | 643, 925, 1000, 1175 |
| Alw21I | GWGCWC | 1 cut(s) | 651 |
| Alw26I | GTCTC | 2 cut(s) | 343, 547 |
| AlwI | GGATC | 3 cut(s) | 44, 875, 888 |
| Ama87I | CYCGRG | 2 cut(s) | 443, 650 |
| AoxI | GGCC | 1 cut(s) | 342 |
| ApaI | GGGCCC | 1 cut(s) | 346 |
| ApoI | RAATTY | 4 cut(s) | 299, 383, 685, 814 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 97, 129, 284, 316 |
| AseI | ATTAAT | 2 cut(s) | 621, 864 |
| AspLEI | GCGC | 4 cut(s) | 27, 255, 772, 1027 |
| AspS9I | GGNCC | 2 cut(s) | 342, 343 |
| AsuHPI | GGTGA | 2 cut(s) | 142, 460 |
| AsuII | TTCGAA | 1 cut(s) | 689 |
| AvaI | CYCGRG | 2 cut(s) | 443, 650 |
| BaeGI | GKGCMC | 1 cut(s) | 346 |
| BamHI | GGATCC | 1 cut(s) | 880 |
| BanII | GRGCYC | 2 cut(s) | 346, 940 |
| Bbv12I | GWGCWC | 1 cut(s) | 651 |
| BbvCI | CCTCAGC | 1 cut(s) | 1176 |
| BccI | CCATC | 6 cut(s) | 120, 400, 500, 815, 1064, 1087 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 527, 561 |
| BciT130I | CCWGG | 2 cut(s) | 776, 1018 |
| BciVI | GTATCC | 1 cut(s) | 757 |
| BclI | TGATCA | 1 cut(s) | 498 |
| BcoDI | GTCTC | 2 cut(s) | 343, 547 |
| BfaI | CTAG | 3 cut(s) | 158, 698, 708 |
| BfmI | CTRYAG | 1 cut(s) | 1049 |
| BfuI | GTATCC | 1 cut(s) | 757 |
| BmcAI | AGTACT | 1 cut(s) | 192 |
| Bme1390I | CCNGG | 2 cut(s) | 776, 1018 |
| BmeT110I | CYCGRG | 2 cut(s) | 443, 650 |
| BmgT120I | GGNCC | 2 cut(s) | 342, 343 |
| BmiI | GGNNCC | 2 cut(s) | 344, 882 |
| BmrFI | CCNGG | 2 cut(s) | 776, 1018 |
| BmrI | ACTGGG | 1 cut(s) | 916 |
| BmsI | GCATC | 1 cut(s) | 743 |
| BmuI | ACTGGG | 1 cut(s) | 916 |
| BoxI | GACNNNNGTC | 1 cut(s) | 146 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 914, 946 |
| BpmI | CTGGAG | 1 cut(s) | 916 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1176 |
| Bpu14I | TTCGAA | 1 cut(s) | 689 |
| BpuEI | CTTGAG | 1 cut(s) | 398 |
| BsaJI | CCNNGG | 2 cut(s) | 444, 1016 |
| BsaWI | WCCGGW | 1 cut(s) | 457 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 6, 36, 59, 89, 342, 372 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 395, 946 |
| Bse1I | ACTGG | 3 cut(s) | 153, 188, 911 |
| Bse3DI | GCAATG | 1 cut(s) | 475 |
| BseBI | CCWGG | 2 cut(s) | 776, 1018 |
| BseDI | CCNNGG | 2 cut(s) | 444, 1016 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 395, 946 |
| BseMI | GCAATG | 1 cut(s) | 475 |
| BseMII | CTCAG | 2 cut(s) | 338, 1167 |
| BseNI | ACTGG | 3 cut(s) | 153, 188, 911 |
| BseRI | GAGGAG | 1 cut(s) | 1193 |
| BseSI | GKGCMC | 1 cut(s) | 346 |
| BsgI | GTGCAG | 1 cut(s) | 531 |
| Bsh1236I | CGCG | 2 cut(s) | 27, 215 |
| BshFI | GGCC | 1 cut(s) | 344 |
| BsiHKAI | GWGCWC | 1 cut(s) | 651 |
| BsiHKCI | CYCGRG | 2 cut(s) | 443, 650 |
| BsiSI | CCGG | 1 cut(s) | 458 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 395, 946 |
| BsmAI | GTCTC | 2 cut(s) | 343, 547 |
| BsnI | GGCC | 1 cut(s) | 344 |
| BsoBI | CYCGRG | 2 cut(s) | 443, 650 |
| Bsp119I | TTCGAA | 1 cut(s) | 689 |
| Bsp120I | GGGCCC | 1 cut(s) | 342 |
| Bsp1286I | GDGCHC | 3 cut(s) | 346, 651, 940 |
| Bsp1407I | TGTACA | 1 cut(s) | 1185 |
| Bsp143I | GATC | 3 cut(s) | 36, 498, 880 |
| BspACI | CCGC | 4 cut(s) | 215, 245, 884, 1081 |
| BspANI | GGCC | 1 cut(s) | 344 |
| BspCNI | CTCAG | 2 cut(s) | 339, 1168 |
| BspFNI | CGCG | 2 cut(s) | 27, 215 |
| BspHI | TCATGA | 1 cut(s) | 892 |
| BspLI | GGNNCC | 2 cut(s) | 344, 882 |
| BspPI | GGATC | 3 cut(s) | 44, 875, 888 |
| BspT104I | TTCGAA | 1 cut(s) | 689 |
| BsrDI | GCAATG | 1 cut(s) | 475 |
| BsrGI | TGTACA | 1 cut(s) | 1185 |
| BsrI | ACTGG | 3 cut(s) | 153, 188, 911 |
| BssECI | CCNNGG | 2 cut(s) | 444, 1016 |
| BssMI | GATC | 3 cut(s) | 36, 498, 880 |
| BssNAI | GTATAC | 1 cut(s) | 1167 |
| Bst1107I | GTATAC | 1 cut(s) | 1167 |
| Bst2UI | CCWGG | 2 cut(s) | 776, 1018 |
| Bst4CI | ACNGT | 3 cut(s) | 112, 259, 830 |
| BstAUI | TGTACA | 1 cut(s) | 1185 |
| BstBI | TTCGAA | 1 cut(s) | 689 |
| BstC8I | GCNNGC | 1 cut(s) | 918 |
| BstDEI | CTNAG | 4 cut(s) | 120, 347, 377, 1176 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 393 |
| BstFNI | CGCG | 2 cut(s) | 27, 215 |
| BstHHI | GCGC | 4 cut(s) | 27, 255, 772, 1027 |
| BstKTI | GATC | 3 cut(s) | 39, 501, 883 |
| BstMAI | GTCTC | 2 cut(s) | 343, 547 |
| BstMBI | GATC | 3 cut(s) | 36, 498, 880 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 731, 776, 922 |
| BstNI | CCWGG | 2 cut(s) | 776, 1018 |
| BstNSI | RCATGY | 2 cut(s) | 139, 288 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 146 |
| BstSCI | CCNGG | 2 cut(s) | 774, 1016 |
| BstSFI | CTRYAG | 1 cut(s) | 1049 |
| BstSLI | GKGCMC | 1 cut(s) | 346 |
| BstUI | CGCG | 2 cut(s) | 27, 215 |
| BstX2I | RGATCY | 1 cut(s) | 880 |
| BstYI | RGATCY | 1 cut(s) | 880 |
| BstZ17I | GTATAC | 1 cut(s) | 1167 |
| BsuI | GTATCC | 1 cut(s) | 757 |
| BsuRI | GGCC | 1 cut(s) | 344 |
| BtsI | GCAGTG | 1 cut(s) | 240 |
| BtsIMutI | CAGTG | 4 cut(s) | 46, 240, 255, 709 |
| Cac8I | GCNNGC | 1 cut(s) | 918 |
| CciI | TCATGA | 1 cut(s) | 892 |
| CfoI | GCGC | 4 cut(s) | 27, 255, 772, 1027 |
| Cfr13I | GGNCC | 2 cut(s) | 342, 343 |
| CseI | GACGC | 1 cut(s) | 221 |
| Csp6I | GTAC | 3 cut(s) | 191, 585, 1186 |
| CviAII | CATG | 5 cut(s) | 136, 285, 425, 893, 1005 |
| CviJI | RGCY | 8 cut(s) | 98, 344, 643, 779, 925, 938, 1000, 1175 |
| CviKI_1 | RGCY | 8 cut(s) | 98, 344, 643, 779, 925, 938, 1000, 1175 |
| CviQI | GTAC | 3 cut(s) | 191, 585, 1186 |
| DdeI | CTNAG | 4 cut(s) | 120, 347, 377, 1176 |
| DpnI | GATC | 3 cut(s) | 38, 500, 882 |
| DpnII | GATC | 3 cut(s) | 36, 498, 880 |
| DraI | TTTAAA | 1 cut(s) | 325 |
| EciI | GGCGGA | 1 cut(s) | 1096 |
| Eco24I | GRGCYC | 2 cut(s) | 346, 940 |
| Eco57I | CTGAAG | 2 cut(s) | 578, 1191 |
| Eco88I | CYCGRG | 2 cut(s) | 443, 650 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 393 |
| EcoO109I | RGGNCCY | 2 cut(s) | 342, 343 |
| EcoRI | GAATTC | 1 cut(s) | 685 |
| EcoRII | CCWGG | 2 cut(s) | 774, 1016 |
| EcoT38I | GRGCYC | 2 cut(s) | 346, 940 |
| FaeI | CATG | 5 cut(s) | 139, 288, 428, 896, 1008 |
| FatI | CATG | 5 cut(s) | 135, 284, 424, 892, 1004 |
| FbaI | TGATCA | 1 cut(s) | 498 |
| FblI | GTMKAC | 1 cut(s) | 1166 |
| FriOI | GRGCYC | 2 cut(s) | 346, 940 |
| FspBI | CTAG | 3 cut(s) | 158, 698, 708 |
| GlaI | GCGC | 4 cut(s) | 26, 254, 771, 1026 |
| GsuI | CTGGAG | 1 cut(s) | 916 |
| HaeIII | GGCC | 1 cut(s) | 344 |
| HapII | CCGG | 1 cut(s) | 458 |
| HgaI | GACGC | 1 cut(s) | 221 |
| HhaI | GCGC | 4 cut(s) | 27, 255, 772, 1027 |
| Hin1II | CATG | 5 cut(s) | 139, 288, 428, 896, 1008 |
| Hin6I | GCGC | 4 cut(s) | 25, 253, 770, 1025 |
| HinP1I | GCGC | 4 cut(s) | 25, 253, 770, 1025 |
| HindIII | AAGCTT | 1 cut(s) | 641 |
| HinfI | GANTC | 4 cut(s) | 307, 783, 1009, 1071 |
| HpaII | CCGG | 1 cut(s) | 458 |
| HphI | GGTGA | 2 cut(s) | 142, 460 |
| Hpy166II | GTNNAC | 4 cut(s) | 141, 185, 451, 1167 |
| Hpy188I | TCNGA | 5 cut(s) | 87, 211, 670, 739, 997 |
| Hpy188III | TCNNGA | 9 cut(s) | 158, 290, 443, 635, 652, 893, 933, 962, 1240 |
| Hpy8I | GTNNAC | 4 cut(s) | 141, 185, 451, 1167 |
| Hpy99I | CGWCG | 1 cut(s) | 170 |
| HpyAV | CCTTC | 3 cut(s) | 88, 431, 1078 |
| HpyCH4III | ACNGT | 3 cut(s) | 112, 259, 830 |
| HpyCH4IV | ACGT | 4 cut(s) | 10, 181, 1128, 1202 |
| HpyCH4V | TGCA | 5 cut(s) | 240, 330, 548, 734, 764 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 731, 776, 922 |
| HpyF3I | CTNAG | 4 cut(s) | 120, 347, 377, 1176 |
| HpySE526I | ACGT | 4 cut(s) | 10, 181, 1128, 1202 |
| Hsp92II | CATG | 5 cut(s) | 139, 288, 428, 896, 1008 |
| HspAI | GCGC | 4 cut(s) | 25, 253, 770, 1025 |
| Ksp22I | TGATCA | 1 cut(s) | 498 |
| Kzo9I | GATC | 3 cut(s) | 36, 498, 880 |
| LmnI | GCTCC | 2 cut(s) | 14, 777 |
| LweI | GCATC | 1 cut(s) | 743 |
| MaeI | CTAG | 3 cut(s) | 158, 698, 708 |
| MaeII | ACGT | 4 cut(s) | 10, 181, 1128, 1202 |
| MaeIII | GTNAC | 5 cut(s) | 65, 148, 427, 1041, 1124 |
| MalI | GATC | 3 cut(s) | 38, 500, 882 |
| MboI | GATC | 3 cut(s) | 36, 498, 880 |
| MboII | GAAGA | 6 cut(s) | 41, 239, 503, 881, 962, 977 |
| MflI | RGATCY | 1 cut(s) | 880 |
| MhlI | GDGCHC | 3 cut(s) | 346, 651, 940 |
| MmeI | TCCRAC | 1 cut(s) | 110 |
| MnlI | CCTC | 9 cut(s) | 88, 96, 321, 333, 399, 439, 561, 648, 1171 |
| MseI | TTAA | 6 cut(s) | 324, 621, 864, 1065, 1115, 1230 |
| MslI | CAYNNNNRTG | 1 cut(s) | 397 |
| MspI | CCGG | 1 cut(s) | 458 |
| MspR9I | CCNGG | 2 cut(s) | 776, 1018 |
| MvaI | CCWGG | 2 cut(s) | 776, 1018 |
| MvnI | CGCG | 2 cut(s) | 27, 215 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 731, 776, 922 |
| NdeII | GATC | 3 cut(s) | 36, 498, 880 |
| NlaIII | CATG | 5 cut(s) | 139, 288, 428, 896, 1008 |
| NlaIV | GGNNCC | 2 cut(s) | 344, 882 |
| NmuCI | GTSAC | 3 cut(s) | 65, 148, 1041 |
| NspI | RCATGY | 2 cut(s) | 139, 288 |
| NspV | TTCGAA | 1 cut(s) | 689 |
| PaeR7I | CTCGAG | 1 cut(s) | 650 |
| PagI | TCATGA | 1 cut(s) | 892 |
| PciI | ACATGT | 2 cut(s) | 135, 284 |
| PfeI | GAWTC | 4 cut(s) | 307, 783, 1009, 1071 |
| PscI | ACATGT | 2 cut(s) | 135, 284 |
| PshAI | GACNNNNGTC | 1 cut(s) | 146 |
| PshBI | ATTAAT | 2 cut(s) | 621, 864 |
| PsiI | TTATAA | 1 cut(s) | 675 |
| Psp1406I | AACGTT | 1 cut(s) | 181 |
| Psp6I | CCWGG | 2 cut(s) | 774, 1016 |
| PspGI | CCWGG | 2 cut(s) | 774, 1016 |
| PspN4I | GGNNCC | 2 cut(s) | 344, 882 |
| PspOMI | GGGCCC | 1 cut(s) | 342 |
| PspPI | GGNCC | 2 cut(s) | 342, 343 |
| PsuI | RGATCY | 1 cut(s) | 880 |
| RsaI | GTAC | 3 cut(s) | 192, 586, 1187 |
| RsaNI | GTAC | 3 cut(s) | 191, 585, 1186 |
| RseI | CAYNNNNRTG | 1 cut(s) | 397 |
| SaqAI | TTAA | 6 cut(s) | 324, 621, 864, 1065, 1115, 1230 |
| Sau3AI | GATC | 3 cut(s) | 36, 498, 880 |
| Sau96I | GGNCC | 2 cut(s) | 342, 343 |
| ScaI | AGTACT | 1 cut(s) | 192 |
| ScrFI | CCNGG | 2 cut(s) | 776, 1018 |
| SduI | GDGCHC | 3 cut(s) | 346, 651, 940 |
| SfaNI | GCATC | 1 cut(s) | 743 |
| SfcI | CTRYAG | 1 cut(s) | 1049 |
| Sfr274I | CTCGAG | 1 cut(s) | 650 |
| SfuI | TTCGAA | 1 cut(s) | 689 |
| SlaI | CTCGAG | 1 cut(s) | 650 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 397 |
| SmlI | CTYRAG | 2 cut(s) | 413, 650 |
| SmoI | CTYRAG | 2 cut(s) | 413, 650 |
| SsiI | CCGC | 4 cut(s) | 215, 245, 884, 1081 |
| SspI | AATATT | 1 cut(s) | 992 |
| SspMI | CTAG | 3 cut(s) | 158, 698, 708 |
| StyD4I | CCNGG | 2 cut(s) | 774, 1016 |
| TaaI | ACNGT | 3 cut(s) | 112, 259, 830 |
| TaiI | ACGT | 4 cut(s) | 13, 184, 1131, 1205 |
| TaqI | TCGA | 4 cut(s) | 165, 537, 651, 689 |
| TatI | WGTACW | 2 cut(s) | 190, 1185 |
| TfiI | GAWTC | 4 cut(s) | 307, 783, 1009, 1071 |
| Tru1I | TTAA | 6 cut(s) | 324, 621, 864, 1065, 1115, 1230 |
| Tru9I | TTAA | 6 cut(s) | 324, 621, 864, 1065, 1115, 1230 |
| TscAI | CASTG | 4 cut(s) | 46, 247, 262, 709 |
| TseFI | GTSAC | 3 cut(s) | 65, 148, 1041 |
| Tsp45I | GTSAC | 3 cut(s) | 65, 148, 1041 |
| TspGWI | ACGGA | 1 cut(s) | 350 |
| TspRI | CASTG | 4 cut(s) | 46, 247, 262, 709 |
| VspI | ATTAAT | 2 cut(s) | 621, 864 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 393 |
| XapI | RAATTY | 4 cut(s) | 299, 383, 685, 814 |
| XbaI | TCTAGA | 1 cut(s) | 157 |
| XceI | RCATGY | 2 cut(s) | 139, 288 |
| XhoI | CTCGAG | 1 cut(s) | 650 |
| XmiI | GTMKAC | 1 cut(s) | 1166 |
| XspI | CTAG | 3 cut(s) | 158, 698, 708 |
| ZrmI | AGTACT | 1 cut(s) | 192 |