FvH4_7g04391

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000143
Physical Location & Seq
Forward (+)
4968989 .. 4977863
8875 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g04391.t1

Sequence Viewer

Length: 3492 bp
ATGCTGAACATGGATCCCAACGAGACCGAACAGAAGCTTCTCCCTCAGGCTAGAATTGTGGGAAACATGGAGGCAGAGACTGTGGTCTGTACCACAGACCAACTTATTGAAGAAGAGCCACAATACACACATATTCACCAGAATGAATTCTCATATAGGAGGCACAAGAAGCAGGAGGAGGAGGATATTGTCATATGTGAATGCAAGTATGATGAAAAGGATCCTGAAAGTGCATGTGGAGAAAGGTGCTTGAATGTGTTGACCAGCACGGAGTGCACTCCTGGATATTGCCCTTGCGGTGTGTATTGTAAGAATCAGAGATTTCAGAAACGTGAATATGCCAAGATAAAGTTGTTTAAAACAGAAGGCTGTGGTTGGGGCCTTTTAGCCAATGAGAACATAAAGGCAGGGCAGTTTATTATCGAGTACTGTGGAGAAGTGATATCATGGAAAGAAGCAAAGAAAAGATCGCATGCTTATGAAATTGAAGGTATCAGAGATGCATTTATTATTTCTCTCAATGCCTCTGAGTCTATCGATGCCACTGTGAAGGGAAGCCTTGCCAGATTTATAAATCATTCTTGCCAACCAAATTGTGAGACAAGAAAGTGGAATGTGCTTGGGGAAATTAGGGTTGGAATATTTGCAAAACAAGATATATTAGTTGGAACTGAGTTGGCATATGACTACAATTTTGAATGGTATGGAGGTGTTAAGGTTCGCTGCCTCTGTGGTGCACCTAGCTGTTCAGGATTTCTTGGGGCAAAATCTCGTGGTTTCCAGGGTGATACTTCTAGTCGAGGTAGTGGGGTTGGGTCGGATACTGCGATGGATTCCATGATTCAATCTAGTACTCATGAGGTCGATACTTCTACTCGAGGTAGTGAGGTTGACTCGGAATGTGTAATGGATTTCACAAGTGAATTTACTACTTATGAGATTTTCAAGAGTAGAGAATCCTTGATGCTGTGGGCTCGTGAGGCTGGGAAACGAAACGGGTTTGTTGTCGTGACAGTACGATCTGATTTGGGTGGTAAAAGTTTGAAACCAAGAGTTACATTAGGGTGTGAGCGTGGTGGAAAATTTAAGGTTCACAAAGAAAGATGTGATAAAGGTGGGAAGCAAAGATGTGGGACTGGCAGCAAGAAGTGTAGCTGTCCTTTCACATTAAAGGGAGAGAAGTTAACAACTGGTGATGGATGGATGCTAAATGTAGTATGTGGAGTGCATAATCATGCGTTGACCGAGACTCTTGAAGGGCACTCGTATGCAGGGAGGTTGTCTGAGGAGGAGACTTCACTCTTGATAGACTTGTCAAAGAGTCTAGTACGACCCAAAGAAATCTTGAACAAATTGAAGCAAAGGGATTCACTCAATGTTACAACTATAAGAACTATATACAATGCACGCCAGAGGCAAAGGGTTAAAGATAAAGCAGGGAAGTCTCAAATGGAACAGCTTTTATCGAAGTTAAATGAGCATAATTACCTTGAGTGGCACAGGAGTTGCCCTATCTCAGAGAGTATGCTGGACTTGTTTTGGGCACACCCTGTGAGTCTGGAATTGTTACGTGTATTTCCTACCATCTTGATGATGGATTGCACTTTTAAAACCAATAGATACCGTTATCCTCTCCTAGAGATTGTAGGTGTGACATCTACTAATATGACATTCTCAATTGCATTTGTATATTTGGATTCCGAAAAAGAAGATAATTATGTGTGGGCCTTAAATAGATTACGTGATCTGTTGGATGGGTGTGCTATGCCCAATGTTATCGTCACAGACAAGGATTTGGAATTGATGAGAGCTGTTGAGACGGTATTTCCTTCAGCTAGACATATGCTGTGTAAATGGCATATCAATAAGCATGTGTTCGCCAAATGCAGAAATTTGTTTGAGTCCAAGGATAAGTGGGATAGGTTTATCATGAAGTGGAATGTCCTTGTGACGTCACCTACGGAAGAAGACTACATACAAGAGCTAGCAGTGCTACACAGTGAATTTAGTACTTACACAGAGGTCTTGGACTATGTTACAAACACTTGGCTGAATCCTTTCAAGGACAGATTTGTGTCGGCCTGGACAGACATGATCATGCATTTTGGTAATGTGGCATCAAACAGGGCGGAGAGTTCACATTCTAAGCTTAAGAGGCAACTTGGTTTGAGTCAGGGGAATTTTGAGGGTTCTTTTGAGAAAATACATGCTCTTCTTGAGTTACAACACTCAGACATTAAGGCTTCTTTCGAGAAATGTTTGACAGTTGTCCGACATAGCTCCTGGCCTTCAGAATTCAAACATCTAAGAGGAGTAGTTTCTATGTTTGCATTGGACATGGTATTGTCTCAGTTCAAGCTAAGCACTTCAATTGAAATTGATGATCTTCATTGTGGTTGTGTCATACGCCGCACCCATGGTTTACCTTGTGCCCATGAAATTGCCAAATATAAGAAGGATTGTCGACCAGTTCCGCTCGAGTCTGTTGAACCCCATTGGAGGAAACTTCATATGTCACCCCCTTCTGATCTCATTAATAATACTATGGATGTTAATGAATTTCCTGAGTTGGAGTTGCTTCAACAGTACTTTTTTAAGACAAGTCCTGATAATCGGTTGACTCTGCGGAAAAGAGTGAGAGAACTTGTACCATCCCTTGCAACTCTTGTTGAACCAAAGGTTAAGTCAAGAACACGAGGTCAATTGAAGGATATAATTGATATGTCCATTCAGAAAAAAGCATCTGCAATTGAGCTAATACAATCTGCGTCAGTGGACAGTCTTTCACCTAGTGTGACTCGTGATACAATGGAAGTTTTGGAAAAATCAACACATAAGCAAACAGGGCCAATGAAAGAGAAGGTTTACCGGACACTCTCAGCAAAAATTATGTCGTATATCGATTCATTTCCAGCTGGATTGGGTCCATACATAGACGAAGTCAAGGATGTATGTGATGATGGTAATTGTGGGTTTAGAGCGGTTGCTGATTTAGTAGGATTGGGTGAGAATGCATACTTGCAAGTGAGGAATGACCTACTGACTGAATTGTCTTCCTATTCTGCGCATTATGGTGAGCTATATGGCAGCGCAGAGAGGGTTCGTGAGTTGGCACAATCACTTTCATTCTTTAATGAGGGTAGAGCGCCATTCGACAGATGTATGACAATGCCTGACATGGGTCATCTCATAGCATCATGCTATAAGATTGTACTCTTCTACTTGTCAGCTACTCAATGCCTTACCTTCCTACCCCTTCGATCAACACCTGTTCCTTTGCCAATGCGTAGACATATTGCTGTTGGTCTTGTCAATGATTCCCATTACGTTGAGGTCTTCTTAAAGCCAGGACATCCTGTACCACCCATTGAGTGTAATTGGATCAGATATCGTACACAAGCTGCACAAGGGTGGGATACTATATATACTGATTGTATTGCACACTTCCAGTCAGGTGTGAATGGAGCTGGAGAAGTTGCAAAACATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
FAR1 MULE SET

Protein Analysis

1164

Amino Acids

131.99

Weight (kDa)

6.85

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWS PF17907 74 - 111 8e-15 AWS domain
SET PF00856 125 - 231 2.3e-24 SET domain
ZSWIM1-3_RNaseH-like PF21056 521 - 614 2.5e-11 Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE PF10551 530 - 623 1.1e-24 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76710 AT1G76710 AT1G76710 AT1G76710
fragaria_vesca FvH4_1g20063 FvH4_2g15231 FvH4_3g04011 FvH4_3g28681 FvH4_4g01411 FvH4_4g10181 FvH4_4g15922 FvH4_4g16651 FvH4_4g16652 FvH4_5g13892 FvH4_5g13893 FvH4_5g20623 FvH4_5g20623 FvH4_5g20623 FvH4_5g20623 FvH4_5g25511 FvH4_6g12551 FvH4_6g12552 FvH4_6g38841 FvH4_7g04391
malus_domestica MD05G1031300.v1.1 MD05G1031400.v1.1 MD05G1031500.v1.1 MD08G1164400.v1.1 MD10G1032900.v1.1 MD10G1033000.v1.1 MD10G1033100.v1.1
prunus_persica Prupe.3G240700_v2.0.a1 Prupe.7G144600_v2.0.a1 Prupe.8G038500_v2.0.a1 Prupe.8G038500_v2.0.a1
pyrus_communis pycom05g02250 pycom05g02260 pycom05g02270 pycom05g02280 pycom08g14160 pycom10g02100 pycom10g02110 pycom10g02130
rosa_chinensis RchiOBHm_Chr1g0329481 RchiOBHm_Chr1g0329491 RchiOBHm_Chr1g0341531 RchiOBHm_Chr3g0464811 RchiOBHm_Chr3g0494701 RchiOBHm_Chr3g0497501 RchiOBHm_Chr4g0385841 RchiOBHm_Chr5g0061181 RchiOBHm_Chr6g0263001 RchiOBHm_Chr7g0193111 RchiOBHm_Chr7g0206281 RchiOBHm_Chr7g0206311
rosa_laevigata RLG00000018908 RLG00000028528 RLG00000028803 RLG00000029882 RLG00000029883 RLG00000030361
rosa_multiflora Rmu_co8196328.1_g000001 Rmu_co8259135.1_g000001 Rmu_co8269185.1_g000001 Rmu_sc0000076.1_g000019 Rmu_sc0000311.1_g000007 Rmu_sc0000429.1_g000038 Rmu_sc0000429.1_g000039 Rmu_sc0000429.1_g000040 Rmu_sc0000511.1_g000005 Rmu_sc0000539.1_g000054 Rmu_sc0000566.1_g000039 Rmu_sc0000805.1_g000032 Rmu_sc0000870.1_g000073 Rmu_sc0000870.1_g000074 Rmu_sc0001010.1_g000018 Rmu_sc0001083.1_g000013 Rmu_sc0001114.1_g000011 Rmu_sc0001702.1_g000027 Rmu_sc0001779.1_g000012 Rmu_sc0002923.1_g000036 Rmu_sc0003242.1_g000011 Rmu_sc0003291.1_g000036 Rmu_sc0003393.1_g000012 Rmu_sc0003872.1_g000016 Rmu_sc0003872.1_g000017 Rmu_sc0003872.1_g000018 Rmu_sc0004084.1_g000020 Rmu_sc0004386.1_g000005 Rmu_sc0004622.1_g000005 Rmu_sc0004742.1_g000021 Rmu_sc0004755.1_g000020 Rmu_sc0004805.1_g000036 Rmu_sc0005065.1_g000006 Rmu_sc0005177.1_g000004 Rmu_sc0006373.1_g000003 Rmu_sc0006373.1_g000004 Rmu_sc0007121.1_g000002 Rmu_sc0007943.1_g000003 Rmu_sc0010368.1_g000006 Rmu_sc0010543.1_g000001 Rmu_sc0012591.1_g000006 Rmu_sc0013504.1_g000009 Rmu_sc0015025.1_g000001 Rmu_sc0016170.1_g000001 Rmu_sc0017156.1_g000001 Rmu_sc0026439.1_g000001
rosa_roxburghii Rroxscaffold_175G00432280 Rroxscaffold_1G00030190 Rroxscaffold_1G00030880 Rroxscaffold_2G00090520 Rroxscaffold_3G00227940 Rroxscaffold_3G00233400 Rroxscaffold_3G00238500 Rroxscaffold_3G00251650 Rroxscaffold_4G00301570 Rroxscaffold_4G00321040 Rroxscaffold_5G00350880 Rroxscaffold_7G00184400
rosa_rugosa Rorug01G0080800 Rorug01G0080900 Rorug01G0193900 Rorug02G0275900 Rorug02G0275900 Rorug02G0275900 Rorug02G0305400 Rorug02G0311800 Rorug02G0350400 Rorug02G0381300 Rorug03G0136700 Rorug03G0181900 Rorug03G0268000 Rorug04G0301600 Rorug05G0175100 Rorug05G0250700 Rorug05G0298200 Rorug05G0596000 Rorug06G0029500 Rorug07G0094300 Rorug07G0094300 Rorug07G0095400 Rorug07G0140100
rosa_samantha Rh1AG099200 Rh1AG190500 Rh1BG078900 Rh1BG079000 Rh1BG079100 Rh1CG084600 Rh1CG084700 Rh1CG095300 Rh1CG175900 Rh1DG103300 Rh1DG150400 Rh2AG079300 Rh2AG272400 Rh2AG464800 Rh2AG507400 Rh2DG078300 Rh2DG078400 Rh3CG206900 Rh3CG295400 Rh3DG206800 Rh3DG356200 Rh4CG071500 Rh4CG179200 Rh4DG064700 Rh5CG362400 Rh7AG212300 Rh7AG226200 Rh7BG222200
rosa_wichuraiana Rw0G009990 Rw0G011260 Rw0G011270 Rw0G012910 Rw0G013740 Rw1G007740 Rw1G013050 Rw1G014230 Rw2G014270 Rw3G012720 Rw3G028110 Rw4G006630 Rw4G015270 Rw4G016160 Rw4G031380 Rw5G011440 Rw5G029180 Rw5G048360 Rw5G048430 Rw5G048780 Rw6G005250 Rw6G006390 Rw7G002840 Rw7G014080 Rw7G019650 Rw7G032790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 572
AasI GACNNNNNNGTC 1 cut(s) 3049
AatII GACGTC 1 cut(s) 1955
Acc16I TGCGCA 1 cut(s) 3066
AccBSI CCGCTC 2 cut(s) 2476, 2981
AccI GTMKAC 2 cut(s) 2464, 3289
AciI CCGC 6 cut(s) 297, 2129, 2410, 2474, 2626, 2981
AclWI GGATC 5 cut(s) 8, 21, 215, 228, 3389
AcsI RAATTY 8 cut(s) 146, 923, 1082, 1891, 2003, 2179, 2294, 2558
AcuI CTGAAG 2 cut(s) 1815, 2274
AcyI GRCGYC 1 cut(s) 1952
AdeI CACNNNGTG 3 cut(s) 273, 1552, 2792
AfiI CCNNNNNNNGG 2 cut(s) 2499, 3179
AflII CTTAAG 1 cut(s) 2150
AflIII ACRYGT 1 cut(s) 1570
AjnI CCWGG 5 cut(s) 280, 780, 2081, 2282, 3346
AjuI GAANNNNNNNTTGG 2 cut(s) 618, 650
AleI CACNNNNGTG 1 cut(s) 3409
Alw21I GWGCWC 2 cut(s) 278, 739
Alw26I GTCTC 8 cut(s) 17, 71, 593, 1241, 1286, 1449, 1811, 2352
Alw44I GTGCAC 2 cut(s) 274, 735
AlwI GGATC 5 cut(s) 8, 21, 215, 228, 3389
AlwNI CAGNNNCTG 1 cut(s) 80
Ama87I CYCGRG 2 cut(s) 876, 2477
AoxI GGCC 5 cut(s) 379, 1725, 2079, 2285, 2846
ApaLI GTGCAC 2 cut(s) 274, 735
ApeKI GCWGC 4 cut(s) 723, 1140, 3087, 3401
ApoI RAATTY 8 cut(s) 146, 923, 1082, 1891, 2003, 2179, 2294, 2558
ArsI GACNNNNNNTTYG 6 cut(s) 677, 709, 2669, 2701, 2876, 2908
AseI ATTAAT 1 cut(s) 2535
Asp700I GAANNNNTTC 2 cut(s) 146, 2057
AspLEI GCGC 3 cut(s) 3067, 3092, 3148
AspS9I GGNCC 4 cut(s) 379, 1725, 2846, 2924
AsuHPI GGTGA 8 cut(s) 128, 797, 1205, 1947, 2508, 2778, 3017, 3086
AsuNHI GCTAGC 1 cut(s) 1984
AvaI CYCGRG 2 cut(s) 876, 2477
AvaII GGWCC 1 cut(s) 2924
AxyI CCTNAGG 1 cut(s) 45
BaeGI GKGCMC 5 cut(s) 278, 739, 1263, 1546, 2434
BamHI GGATCC 2 cut(s) 13, 220
BanII GRGCYC 1 cut(s) 976
BarI GAAGNNNNNNTAC 2 cut(s) 1959, 1991
BauI CACGAG 4 cut(s) 771, 975, 2694, 2799
BbsI GAAGAC 3 cut(s) 1974, 3045, 3328
Bbv12I GWGCWC 2 cut(s) 278, 739
BbvI GCAGC 4 cut(s) 710, 1152, 3099, 3388
BccI CCATC 8 cut(s) 823, 1190, 1194, 1588, 1592, 1748, 2659, 2954
BciT130I CCWGG 5 cut(s) 282, 782, 2083, 2284, 3348
BciVI GTATCC 2 cut(s) 814, 3409
BclI TGATCA 1 cut(s) 2094
BcoDI GTCTC 8 cut(s) 17, 71, 593, 1241, 1286, 1449, 1811, 2352
BfaI CTAG 9 cut(s) 51, 741, 795, 849, 1325, 1637, 1836, 1985, 2790
BfoI RGCGCY 1 cut(s) 3149
BfrI CTTAAG 1 cut(s) 2150
BfuI GTATCC 2 cut(s) 814, 3409
BisI GCNGC 5 cut(s) 724, 1141, 2410, 3088, 3402
BlpI GCTNAGC 1 cut(s) 2360
BlsI GCNGC 5 cut(s) 725, 1142, 2411, 3089, 3403
BmcAI AGTACT 4 cut(s) 428, 853, 2011, 2588
Bme1390I CCNGG 5 cut(s) 282, 782, 2083, 2284, 3348
Bme18I GGWCC 1 cut(s) 2924
BmeT110I CYCGRG 2 cut(s) 876, 2477
BmgT120I GGNCC 4 cut(s) 379, 1725, 2846, 2924
BmiI GGNNCC 4 cut(s) 15, 222, 380, 2925
BmrFI CCNGG 5 cut(s) 282, 782, 2083, 2284, 3348
BmsI GCATC 7 cut(s) 490, 529, 954, 1194, 2126, 2750, 3203
BmtI GCTAGC 1 cut(s) 1988
BoxI GACNNNNGTC 3 cut(s) 83, 2267, 3180
BpiI GAAGAC 3 cut(s) 1974, 3045, 3328
BplI GAGNNNNNCTC 2 cut(s) 878, 910
BpmI CTGGAG 1 cut(s) 3489
Bpu1102I GCTNAGC 1 cut(s) 2360
BpuEI CTTGAG 2 cut(s) 1511, 2237
Bsa29I ATCGAT 2 cut(s) 537, 2901
BsaAI YACGTR 2 cut(s) 1571, 1742
BsaHI GRCGYC 1 cut(s) 1952
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 3 cut(s) 781, 1905, 2416
BsaWI WCCGGW 1 cut(s) 2868
Bsc4I CCNNNNNNNGG 2 cut(s) 2499, 3179
Bse1I ACTGG 4 cut(s) 1141, 1195, 2468, 3448
Bse21I CCTNAGG 1 cut(s) 45
BseBI CCWGG 5 cut(s) 282, 782, 2083, 2284, 3348
BseCI ATCGAT 2 cut(s) 537, 2901
BseDI CCNNGG 3 cut(s) 781, 1905, 2416
BseGI GGATG 7 cut(s) 1205, 1209, 1759, 2554, 2651, 2953, 3352
BseLI CCNNNNNNNGG 2 cut(s) 2499, 3179
BseMII CTCAG 9 cut(s) 59, 519, 663, 1275, 1530, 2244, 2363, 2556, 2892
BseNI ACTGG 4 cut(s) 1141, 1195, 2468, 3448
BseRI GAGGAG 5 cut(s) 191, 194, 1301, 1304, 2325
BseSI GKGCMC 5 cut(s) 278, 739, 1263, 1546, 2434
BseXI GCAGC 4 cut(s) 710, 1152, 3099, 3388
BseYI CCCAGC 1 cut(s) 983
BsgI GTGCAG 1 cut(s) 3387
BshFI GGCC 5 cut(s) 381, 1727, 2081, 2287, 2848
BshVI ATCGAT 2 cut(s) 537, 2901
BsiHKAI GWGCWC 2 cut(s) 278, 739
BsiHKCI CYCGRG 2 cut(s) 876, 2477
BsiSI CCGG 1 cut(s) 2869
BslFI GGGAC 1 cut(s) 1147
BslI CCNNNNNNNGG 2 cut(s) 2499, 3179
BsmAI GTCTC 8 cut(s) 17, 71, 593, 1241, 1286, 1449, 1811, 2352
BsmBI CGTCTC 1 cut(s) 1811
BsmFI GGGAC 1 cut(s) 1147
BsmI GAATGC 2 cut(s) 206, 3016
BsnI GGCC 5 cut(s) 381, 1727, 2081, 2287, 2848
Bso31I GGTCTC 1 cut(s) 17
BsoBI CYCGRG 2 cut(s) 876, 2477
Bsp1286I GDGCHC 6 cut(s) 278, 739, 976, 1263, 1546, 2434
Bsp1720I GCTNAGC 1 cut(s) 2360
Bsp19I CCATGG 1 cut(s) 2416
BspACI CCGC 6 cut(s) 297, 2129, 2410, 2474, 2626, 2981
BspANI GGCC 5 cut(s) 381, 1727, 2081, 2287, 2848
BspCNI CTCAG 9 cut(s) 58, 520, 664, 1276, 1529, 2243, 2362, 2557, 2891
BspDI ATCGAT 2 cut(s) 537, 2901
BspHI TCATGA 2 cut(s) 856, 1929
BspLI GGNNCC 4 cut(s) 15, 222, 380, 2925
BspOI GCTAGC 1 cut(s) 1988
BspPI GGATC 5 cut(s) 8, 21, 215, 228, 3389
BspQI GCTCTTC 2 cut(s) 108, 2217
BspTI CTTAAG 1 cut(s) 2150
BspTNI GGTCTC 1 cut(s) 17
BsrBI CCGCTC 2 cut(s) 2476, 2981
BsrI ACTGG 4 cut(s) 1141, 1195, 2468, 3448
BssECI CCNNGG 3 cut(s) 781, 1905, 2416
BssNI GRCGYC 1 cut(s) 1952
BssSI CACGAG 4 cut(s) 771, 975, 2694, 2799
BssT1I CCWWGG 2 cut(s) 1905, 2416
Bst2BI CACGAG 4 cut(s) 771, 975, 2694, 2799
Bst2UI CCWGG 5 cut(s) 282, 782, 2083, 2284, 3348
Bst6I CTCTTC 3 cut(s) 108, 2217, 3221
BstACI GRCGYC 1 cut(s) 1952
BstAFI CTTAAG 1 cut(s) 2150
BstAPI GCANNNNNTGC 2 cut(s) 273, 3485
BstBAI YACGTR 2 cut(s) 1571, 1742
BstC8I GCNNGC 3 cut(s) 474, 1408, 1986
BstDSI CCRYGG 1 cut(s) 2416
BstF5I GGATG 7 cut(s) 1205, 1209, 1759, 2554, 2651, 2953, 3352
BstH2I RGCGCY 1 cut(s) 3149
BstHHI GCGC 3 cut(s) 3067, 3092, 3148
BstMAI GTCTC 8 cut(s) 17, 71, 593, 1241, 1286, 1449, 1811, 2352
BstMWI GCNNNNNNNGC 7 cut(s) 169, 273, 980, 1990, 2155, 2845, 3485
BstNI CCWGG 5 cut(s) 282, 782, 2083, 2284, 3348
BstNSI RCATGY 5 cut(s) 237, 476, 1874, 2210, 3488
BstPAI GACNNNNGTC 3 cut(s) 83, 2267, 3180
BstSCI CCNGG 5 cut(s) 280, 780, 2081, 2282, 3346
BstSLI GKGCMC 5 cut(s) 278, 739, 1263, 1546, 2434
BstV1I GCAGC 4 cut(s) 710, 1152, 3099, 3388
BstV2I GAAGAC 3 cut(s) 1974, 3045, 3328
BstX2I RGATCY 2 cut(s) 13, 220
BstYI RGATCY 2 cut(s) 13, 220
Bsu15I ATCGAT 2 cut(s) 537, 2901
Bsu36I CCTNAGG 1 cut(s) 45
BsuI GTATCC 2 cut(s) 814, 3409
BsuRI GGCC 5 cut(s) 381, 1727, 2081, 2287, 2848
BsuTUI ATCGAT 2 cut(s) 537, 2901
BtgI CCRYGG 1 cut(s) 2416
BtgZI GCGATG 1 cut(s) 842
BtsCI GGATG 7 cut(s) 1205, 1209, 1759, 2554, 2651, 2953, 3352
BtsI GCAGTG 1 cut(s) 1995
BtsIMutI CAGTG 4 cut(s) 543, 1995, 2005, 2778
Cac8I GCNNGC 3 cut(s) 474, 1408, 1986
CaiI CAGNNNCTG 1 cut(s) 80
CciI TCATGA 2 cut(s) 856, 1929
CfoI GCGC 3 cut(s) 3067, 3092, 3148
Cfr13I GGNCC 4 cut(s) 379, 1725, 2846, 2924
ClaI ATCGAT 2 cut(s) 537, 2901
CseI GACGC 1 cut(s) 2757
DraI TTTAAA 2 cut(s) 358, 1609
DraIII CACNNNGTG 3 cut(s) 273, 1552, 2792
DrdI GACNNNNNNGTC 1 cut(s) 3049
DseDI GACNNNNNNGTC 1 cut(s) 3049
Eam1104I CTCTTC 3 cut(s) 108, 2217, 3221
EarI CTCTTC 3 cut(s) 108, 2217, 3221
EciI GGCGGA 1 cut(s) 2144
Eco130I CCWWGG 2 cut(s) 1905, 2416
Eco24I GRGCYC 1 cut(s) 976
Eco31I GGTCTC 1 cut(s) 17
Eco32I GATATC 2 cut(s) 444, 3389
Eco47I GGWCC 1 cut(s) 2924
Eco57I CTGAAG 2 cut(s) 1815, 2274
Eco81I CCTNAGG 1 cut(s) 45
Eco88I CYCGRG 2 cut(s) 876, 2477
EcoO109I RGGNCCY 1 cut(s) 379
EcoRI GAATTC 2 cut(s) 146, 2294
EcoRII CCWGG 5 cut(s) 280, 780, 2081, 2282, 3346
EcoRV GATATC 2 cut(s) 444, 3389
EcoT14I CCWWGG 2 cut(s) 1905, 2416
EcoT22I ATGCAT 3 cut(s) 505, 2103, 3016
EcoT38I GRGCYC 1 cut(s) 976
ErhI CCWWGG 2 cut(s) 1905, 2416
Esp3I CGTCTC 1 cut(s) 1811
FaqI GGGAC 1 cut(s) 1147
FauNDI CATATG 4 cut(s) 194, 682, 1842, 2511
FbaI TGATCA 1 cut(s) 2094
FblI GTMKAC 2 cut(s) 2464, 3289
Fnu4HI GCNGC 5 cut(s) 724, 1141, 2410, 3088, 3402
FokI GGATG 7 cut(s) 1212, 1216, 1766, 2561, 2638, 2960, 3339
FriOI GRGCYC 1 cut(s) 976
Fsp4HI GCNGC 5 cut(s) 724, 1141, 2410, 3088, 3402
FspBI CTAG 9 cut(s) 51, 741, 795, 849, 1325, 1637, 1836, 1985, 2790
FspI TGCGCA 1 cut(s) 3066
GlaI GCGC 3 cut(s) 3066, 3091, 3147
GluI GCNGC 5 cut(s) 724, 1141, 2410, 3088, 3402
GsaI CCCAGC 1 cut(s) 987
GsuI CTGGAG 1 cut(s) 3489
HaeII RGCGCY 1 cut(s) 3149
HaeIII GGCC 5 cut(s) 381, 1727, 2081, 2287, 2848
HapII CCGG 1 cut(s) 2869
HgaI GACGC 1 cut(s) 2757
HhaI GCGC 3 cut(s) 3067, 3092, 3148
Hin1I GRCGYC 1 cut(s) 1952
Hin6I GCGC 3 cut(s) 3065, 3090, 3146
HinP1I GCGC 3 cut(s) 3065, 3090, 3146
HincII GTYRAC 6 cut(s) 261, 892, 1185, 1242, 2465, 2619
HindII GTYRAC 6 cut(s) 261, 892, 1185, 1242, 2465, 2619
HindIII AAGCTT 2 cut(s) 35, 2147
HpaI GTTAAC 1 cut(s) 1185
HpaII CCGG 1 cut(s) 2869
HphI GGTGA 8 cut(s) 128, 797, 1205, 1947, 2508, 2778, 3017, 3086
HpyCH4IV ACGT 5 cut(s) 331, 1570, 1741, 1952, 3327
HpyF10VI GCNNNNNNNGC 7 cut(s) 169, 273, 980, 1990, 2155, 2845, 3485
HpySE526I ACGT 5 cut(s) 331, 1570, 1741, 1952, 3327
Hsp92I GRCGYC 1 cut(s) 1952
HspAI GCGC 3 cut(s) 3065, 3090, 3146
Ksp22I TGATCA 1 cut(s) 2094
KspAI GTTAAC 1 cut(s) 1185
LguI GCTCTTC 2 cut(s) 108, 2217
LmnI GCTCC 2 cut(s) 2285, 3464
Lsp1109I GCAGC 4 cut(s) 710, 1152, 3099, 3388
LweI GCATC 7 cut(s) 490, 529, 954, 1194, 2126, 2750, 3203
MaeI CTAG 9 cut(s) 51, 741, 795, 849, 1325, 1637, 1836, 1985, 2790
MaeII ACGT 5 cut(s) 331, 1570, 1741, 1952, 3327
MbiI CCGCTC 2 cut(s) 2476, 2981
MfeI CAATTG 4 cut(s) 1677, 2370, 2702, 2748
MflI RGATCY 2 cut(s) 13, 220
MhlI GDGCHC 6 cut(s) 278, 739, 976, 1263, 1546, 2434
MmeI TCCRAC 6 cut(s) 616, 646, 798, 1731, 2296, 2550
Mph1103I ATGCAT 3 cut(s) 505, 2103, 3016
MroXI GAANNNNTTC 2 cut(s) 146, 2057
MslI CAYNNNNRTG 9 cut(s) 141, 477, 1063, 1233, 1266, 1589, 2096, 3072, 3409
MspA1I CMGCKG 1 cut(s) 2915
MspCI CTTAAG 1 cut(s) 2150
MspI CCGG 1 cut(s) 2869
MspR9I CCNGG 5 cut(s) 282, 782, 2083, 2284, 3348
MunI CAATTG 4 cut(s) 1677, 2370, 2702, 2748
Mva1269I GAATGC 2 cut(s) 206, 3016
MvaI CCWGG 5 cut(s) 282, 782, 2083, 2284, 3348
MwoI GCNNNNNNNGC 7 cut(s) 169, 273, 980, 1990, 2155, 2845, 3485
NcoI CCATGG 1 cut(s) 2416
NdeI CATATG 4 cut(s) 194, 682, 1842, 2511
NheI GCTAGC 1 cut(s) 1984
NlaIV GGNNCC 4 cut(s) 15, 222, 380, 2925
NmuCI GTSAC 7 cut(s) 1009, 1651, 1780, 1948, 1953, 2514, 2794
NsbI TGCGCA 1 cut(s) 3066
NsiI ATGCAT 3 cut(s) 505, 2103, 3016
NspI RCATGY 5 cut(s) 237, 476, 1874, 2210, 3488
OliI CACNNNNGTG 1 cut(s) 3409
PaeI GCATGC 1 cut(s) 476
PaeR7I CTCGAG 2 cut(s) 876, 2477
PagI TCATGA 2 cut(s) 856, 1929
PciSI GCTCTTC 2 cut(s) 108, 2217
PcsI WCGNNNNNNNCGW 1 cut(s) 824
PctI GAATGC 2 cut(s) 206, 3016
PdmI GAANNNNTTC 2 cut(s) 146, 2057
PfeI GAWTC 9 cut(s) 313, 833, 841, 956, 1367, 1697, 2053, 2903, 3317
PflFI GACNNNGTC 1 cut(s) 2939
PfoI TCCNGGA 1 cut(s) 280
PkrI GCNGC 5 cut(s) 725, 1142, 2411, 3089, 3403
Ppu21I YACGTR 2 cut(s) 1571, 1742
PshAI GACNNNNGTC 3 cut(s) 83, 2267, 3180
PshBI ATTAAT 1 cut(s) 2535
PsiI TTATAA 1 cut(s) 572
Psp6I CCWGG 5 cut(s) 280, 780, 2081, 2282, 3346
PspFI CCCAGC 1 cut(s) 983
PspGI CCWGG 5 cut(s) 280, 780, 2081, 2282, 3346
PspN4I GGNNCC 4 cut(s) 15, 222, 380, 2925
PspPI GGNCC 4 cut(s) 379, 1725, 2846, 2924
PspXI VCTCGAGB 2 cut(s) 876, 2477
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 2 cut(s) 13, 220
PsyI GACNNNGTC 1 cut(s) 2939
PvuII CAGCTG 1 cut(s) 2915
RseI CAYNNNNRTG 9 cut(s) 141, 477, 1063, 1233, 1266, 1589, 2096, 3072, 3409
SalI GTCGAC 1 cut(s) 2463
SapI GCTCTTC 2 cut(s) 108, 2217
SatI GCNGC 5 cut(s) 724, 1141, 2410, 3088, 3402
Sau96I GGNCC 4 cut(s) 379, 1725, 2846, 2924
ScaI AGTACT 4 cut(s) 428, 853, 2011, 2588
ScrFI CCNGG 5 cut(s) 282, 782, 2083, 2284, 3348
SduI GDGCHC 6 cut(s) 278, 739, 976, 1263, 1546, 2434
SfaNI GCATC 7 cut(s) 490, 529, 954, 1194, 2126, 2750, 3203
Sfr274I CTCGAG 2 cut(s) 876, 2477
SinI GGWCC 1 cut(s) 2924
SlaI CTCGAG 2 cut(s) 876, 2477
SmiMI CAYNNNNRTG 9 cut(s) 141, 477, 1063, 1233, 1266, 1589, 2096, 3072, 3409
SmlI CTYRAG 5 cut(s) 876, 1490, 2150, 2216, 2477
SmoI CTYRAG 5 cut(s) 876, 1490, 2150, 2216, 2477
SphI GCATGC 1 cut(s) 476
SsiI CCGC 6 cut(s) 297, 2129, 2410, 2474, 2626, 2981
SspI AATATT 1 cut(s) 642
SspMI CTAG 9 cut(s) 51, 741, 795, 849, 1325, 1637, 1836, 1985, 2790
StyD4I CCNGG 5 cut(s) 280, 780, 2081, 2282, 3346
StyI CCWWGG 2 cut(s) 1905, 2416
TaiI ACGT 5 cut(s) 334, 1573, 1744, 1955, 3330
TaqII GACCGA 2 cut(s) 41, 1259
TatI WGTACW 5 cut(s) 426, 851, 2009, 2586, 3211
TauI GCSGC 1 cut(s) 2412
TfiI GAWTC 9 cut(s) 313, 833, 841, 956, 1367, 1697, 2053, 2903, 3317
TscAI CASTG 4 cut(s) 550, 1995, 2005, 2778
TseFI GTSAC 7 cut(s) 1009, 1651, 1780, 1948, 1953, 2514, 2794
TseI GCWGC 4 cut(s) 723, 1140, 3087, 3401
Tsp45I GTSAC 7 cut(s) 1009, 1651, 1780, 1948, 1953, 2514, 2794
TspGWI ACGGA 2 cut(s) 284, 1976
TspRI CASTG 4 cut(s) 550, 1995, 2005, 2778
Tth111I GACNNNGTC 1 cut(s) 2939
Vha464I CTTAAG 1 cut(s) 2150
VneI GTGCAC 2 cut(s) 274, 735
VpaK11BI GGWCC 1 cut(s) 2924
VspI ATTAAT 1 cut(s) 2535
XapI RAATTY 8 cut(s) 146, 923, 1082, 1891, 2003, 2179, 2294, 2558
XceI RCATGY 5 cut(s) 237, 476, 1874, 2210, 3488
XcmI CCANNNNNNNNNTGG 1 cut(s) 1591
XhoI CTCGAG 2 cut(s) 876, 2477
XmiI GTMKAC 2 cut(s) 2464, 3289
XmnI GAANNNNTTC 2 cut(s) 146, 2057
XspI CTAG 9 cut(s) 51, 741, 795, 849, 1325, 1637, 1836, 1985, 2790
ZraI GACGTC 1 cut(s) 1953
ZrmI AGTACT 4 cut(s) 428, 853, 2011, 2588
Zsp2I ATGCAT 3 cut(s) 505, 2103, 3016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.