pycom05g02260

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000143
Physical Location & Seq
Forward (+)
2597395 .. 2598526
1132 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g02260.1

Sequence Viewer

Length: 1029 bp
ATGAATGCACGCCAAAGGCAAAGAGTTAAAGAGAAGGCAGAGAGGTCTCAAATGCAACAACTGTTACGTAAGTTGAATGAGCATGACTATGTCGAGTTGCATAGGAGGTGCCCTATGTCAGATAGTGTGCAGGACTTGTTTTGGGCGCATCCTGTGAGTTTGGAGTTGTTACGTGCATTTCCAACCATTTTGATGATAGATTACACGTACAAGATCAATAGGTATTATTATCCCCTACTAGAGGTTGTAGGCGTCACATCCACTGATATTACATTCTCAATTGCATTTGTCTTTTTGAATTCTGAAAAAGAAGACAACTATATATGGGCATTAAGTAAATTGCGTGGTGTCATGGTGGAGTGTCCCATGCCAAATGTAATTGTCACAGACATGGATTTGCAATTGATGAGAGCCATTGAGGTAGTATTCCCTACTGCTAAAAATCTTAAATGTAAATGGCATATTAATAAGCATGTGTTGGCCAAATGCAAAAACTTGTTTGAGTCCAAGGAAAGGTGTGATAGATTTATTATGAAGTGGAATGTACTGGTGACCTCTCCTACAGAAAATGATTACATACGGGAGCTGGCAGTGTTACAGAGAGAATTTAGTATGTACACTGAGGTCATGGAATATGTTACAAACACATGGTTGAATCCTTTCAAGGACAGATTTGTGTCAGCTTGGACGGACATGATCATGCATTTTGGTAATGTAACATCAAACAGGGCCAAGGGTGCACATACCAAGCTTAAGAGGGAACTTGGTATGAGCCAGGGGAATTTTGAGGGTTCTTTTGAGAGTATACACTCTCTTATTGAGTTACAGCACACTGACATAAAGATATCTTTTGACAAGTGTTTGATGGTCACACAACAAAGCTTCAAGTCTTCAGAATTCAAATATCTGAGAGGAGTTGTATCTATGTTTGCATTGGAGAAGGTGTCATCTCAGTCCAAACTAGCCACCTCAATTGGTGTTGTGTTATACCCCGAACCCATGGTCTACCTTGTGCCCACGAGATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
FAR1 MULE SET

Protein Analysis

343

Amino Acids

40.07

Weight (kDa)

8.86

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZSWIM1-3_RNaseH-like PF21056 53 - 149 5.2e-10 Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE PF10551 64 - 157 5.9e-22 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76710 AT1G76710 AT1G76710 AT1G76710
fragaria_vesca FvH4_1g20063 FvH4_2g15231 FvH4_3g04011 FvH4_3g28681 FvH4_4g01411 FvH4_4g10181 FvH4_4g15922 FvH4_4g16651 FvH4_4g16652 FvH4_5g13892 FvH4_5g13893 FvH4_5g20623 FvH4_5g20623 FvH4_5g20623 FvH4_5g20623 FvH4_5g25511 FvH4_6g12551 FvH4_6g12552 FvH4_6g38841 FvH4_7g04391
malus_domestica MD05G1031300.v1.1 MD05G1031400.v1.1 MD05G1031500.v1.1 MD08G1164400.v1.1 MD10G1032900.v1.1 MD10G1033000.v1.1 MD10G1033100.v1.1
prunus_persica Prupe.3G240700_v2.0.a1 Prupe.7G144600_v2.0.a1 Prupe.8G038500_v2.0.a1 Prupe.8G038500_v2.0.a1
pyrus_communis pycom05g02250 pycom05g02260 pycom05g02270 pycom05g02280 pycom08g14160 pycom10g02100 pycom10g02110 pycom10g02130
rosa_chinensis RchiOBHm_Chr1g0329481 RchiOBHm_Chr1g0329491 RchiOBHm_Chr1g0341531 RchiOBHm_Chr3g0464811 RchiOBHm_Chr3g0494701 RchiOBHm_Chr3g0497501 RchiOBHm_Chr4g0385841 RchiOBHm_Chr5g0061181 RchiOBHm_Chr6g0263001 RchiOBHm_Chr7g0193111 RchiOBHm_Chr7g0206281 RchiOBHm_Chr7g0206311
rosa_laevigata RLG00000018908 RLG00000028528 RLG00000028803 RLG00000029882 RLG00000029883 RLG00000030361
rosa_multiflora Rmu_co8196328.1_g000001 Rmu_co8259135.1_g000001 Rmu_co8269185.1_g000001 Rmu_sc0000076.1_g000019 Rmu_sc0000311.1_g000007 Rmu_sc0000429.1_g000038 Rmu_sc0000429.1_g000039 Rmu_sc0000429.1_g000040 Rmu_sc0000511.1_g000005 Rmu_sc0000539.1_g000054 Rmu_sc0000566.1_g000039 Rmu_sc0000805.1_g000032 Rmu_sc0000870.1_g000073 Rmu_sc0000870.1_g000074 Rmu_sc0001010.1_g000018 Rmu_sc0001083.1_g000013 Rmu_sc0001114.1_g000011 Rmu_sc0001702.1_g000027 Rmu_sc0001779.1_g000012 Rmu_sc0002923.1_g000036 Rmu_sc0003242.1_g000011 Rmu_sc0003291.1_g000036 Rmu_sc0003393.1_g000012 Rmu_sc0003872.1_g000016 Rmu_sc0003872.1_g000017 Rmu_sc0003872.1_g000018 Rmu_sc0004084.1_g000020 Rmu_sc0004386.1_g000005 Rmu_sc0004622.1_g000005 Rmu_sc0004742.1_g000021 Rmu_sc0004755.1_g000020 Rmu_sc0004805.1_g000036 Rmu_sc0005065.1_g000006 Rmu_sc0005177.1_g000004 Rmu_sc0006373.1_g000003 Rmu_sc0006373.1_g000004 Rmu_sc0007121.1_g000002 Rmu_sc0007943.1_g000003 Rmu_sc0010368.1_g000006 Rmu_sc0010543.1_g000001 Rmu_sc0012591.1_g000006 Rmu_sc0013504.1_g000009 Rmu_sc0015025.1_g000001 Rmu_sc0016170.1_g000001 Rmu_sc0017156.1_g000001 Rmu_sc0026439.1_g000001
rosa_roxburghii Rroxscaffold_175G00432280 Rroxscaffold_1G00030190 Rroxscaffold_1G00030880 Rroxscaffold_2G00090520 Rroxscaffold_3G00227940 Rroxscaffold_3G00233400 Rroxscaffold_3G00238500 Rroxscaffold_3G00251650 Rroxscaffold_4G00301570 Rroxscaffold_4G00321040 Rroxscaffold_5G00350880 Rroxscaffold_7G00184400
rosa_rugosa Rorug01G0080800 Rorug01G0080900 Rorug01G0193900 Rorug02G0275900 Rorug02G0275900 Rorug02G0275900 Rorug02G0305400 Rorug02G0311800 Rorug02G0350400 Rorug02G0381300 Rorug03G0136700 Rorug03G0181900 Rorug03G0268000 Rorug04G0301600 Rorug05G0175100 Rorug05G0250700 Rorug05G0298200 Rorug05G0596000 Rorug06G0029500 Rorug07G0094300 Rorug07G0094300 Rorug07G0095400 Rorug07G0140100
rosa_samantha Rh1AG099200 Rh1AG190500 Rh1BG078900 Rh1BG079000 Rh1BG079100 Rh1CG084600 Rh1CG084700 Rh1CG095300 Rh1CG175900 Rh1DG103300 Rh1DG150400 Rh2AG079300 Rh2AG272400 Rh2AG464800 Rh2AG507400 Rh2DG078300 Rh2DG078400 Rh3CG206900 Rh3CG295400 Rh3DG206800 Rh3DG356200 Rh4CG071500 Rh4CG179200 Rh4DG064700 Rh5CG362400 Rh7AG212300 Rh7AG226200 Rh7BG222200
rosa_wichuraiana Rw0G009990 Rw0G011260 Rw0G011270 Rw0G012910 Rw0G013740 Rw1G007740 Rw1G013050 Rw1G014230 Rw2G014270 Rw3G012720 Rw3G028110 Rw4G006630 Rw4G015270 Rw4G016160 Rw4G031380 Rw5G011440 Rw5G029180 Rw5G048360 Rw5G048430 Rw5G048780 Rw6G005250 Rw6G006390 Rw7G002840 Rw7G014080 Rw7G019650 Rw7G032790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 108
AccI GTMKAC 2 cut(s) 805, 1005
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 4 cut(s) 298, 605, 781, 896
AcuI CTGAAG 1 cut(s) 876
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 3 cut(s) 209, 546, 617
AfiI CCNNNNNNNGG 2 cut(s) 241, 513
AflII CTTAAG 1 cut(s) 752
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 6 cut(s) 76, 298, 655, 664, 886, 901
AjnI CCWGG 1 cut(s) 774
AluBI AGCT 4 cut(s) 586, 683, 751, 882
AluI AGCT 4 cut(s) 586, 683, 751, 882
Alw21I GWGCWC 1 cut(s) 742
Alw26I GTCTC 1 cut(s) 51
Alw44I GTGCAC 1 cut(s) 738
AoxI GGCC 2 cut(s) 480, 729
ApaLI GTGCAC 1 cut(s) 738
ApoI RAATTY 4 cut(s) 298, 605, 781, 896
ArsI GACNNNNNNTTYG 2 cut(s) 987, 1019
AseI ATTAAT 1 cut(s) 465
Asp700I GAANNNNTTC 1 cut(s) 659
AspLEI GCGC 1 cut(s) 148
AspS9I GGNCC 1 cut(s) 729
AsuHPI GGTGA 1 cut(s) 562
BaeGI GKGCMC 3 cut(s) 113, 742, 1017
BalI TGGCCA 1 cut(s) 482
BanI GGYRCC 1 cut(s) 108
BauI CACGAG 1 cut(s) 1018
BbsI GAAGAC 2 cut(s) 318, 882
Bbv12I GWGCWC 1 cut(s) 742
BccI CCATC 1 cut(s) 859
BciT130I CCWGG 1 cut(s) 776
BclI TGATCA 1 cut(s) 696
BcoDI GTCTC 1 cut(s) 51
BfaI CTAG 2 cut(s) 239, 962
BfmI CTRYAG 1 cut(s) 561
BfrI CTTAAG 1 cut(s) 752
Bme1390I CCNGG 1 cut(s) 776
BmgT120I GGNCC 1 cut(s) 729
BmiI GGNNCC 1 cut(s) 110
BmrFI CCNGG 1 cut(s) 776
BmsI GCATC 1 cut(s) 157
BpiI GAAGAC 2 cut(s) 318, 882
BsaAI YACGTR 3 cut(s) 68, 173, 207
BsaHI GRCGYC 1 cut(s) 252
BsaI GGTCTC 1 cut(s) 51
BsaJI CCNNGG 4 cut(s) 507, 732, 775, 999
BsaXI ACNNNNNCTCC 2 cut(s) 929, 959
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 513
Bse1I ACTGG 1 cut(s) 552
BseBI CCWGG 1 cut(s) 776
BseDI CCNNGG 4 cut(s) 507, 732, 775, 999
BseGI GGATG 2 cut(s) 148, 257
BseLI CCNNNNNNNGG 2 cut(s) 241, 513
BseMII CTCAG 3 cut(s) 612, 899, 965
BseNI ACTGG 1 cut(s) 552
BseRI GAGGAG 1 cut(s) 927
BseSI GKGCMC 3 cut(s) 113, 742, 1017
BsgI GTGCAG 1 cut(s) 149
BshFI GGCC 2 cut(s) 482, 731
BshNI GGYRCC 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 742
BslFI GGGAC 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 241, 513
BsmAI GTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 348
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 2 cut(s) 482, 731
Bso31I GGTCTC 1 cut(s) 51
Bsp1286I GDGCHC 3 cut(s) 113, 742, 1017
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 2 cut(s) 213, 696
Bsp19I CCATGG 1 cut(s) 999
BspANI GGCC 2 cut(s) 482, 731
BspCNI CTCAG 3 cut(s) 613, 900, 964
BspLI GGNNCC 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 108
BspTI CTTAAG 1 cut(s) 752
BspTNI GGTCTC 1 cut(s) 51
BsrGI TGTACA 1 cut(s) 615
BsrI ACTGG 1 cut(s) 552
BssECI CCNNGG 4 cut(s) 507, 732, 775, 999
BssMI GATC 2 cut(s) 213, 696
BssNAI GTATAC 1 cut(s) 806
BssNI GRCGYC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 1018
BssT1I CCWWGG 3 cut(s) 507, 732, 999
Bst1107I GTATAC 1 cut(s) 806
Bst2BI CACGAG 1 cut(s) 1018
Bst2UI CCWGG 1 cut(s) 776
Bst4CI ACNGT 1 cut(s) 63
BstACI GRCGYC 1 cut(s) 252
BstAFI CTTAAG 1 cut(s) 752
BstAUI TGTACA 1 cut(s) 615
BstBAI YACGTR 3 cut(s) 68, 173, 207
BstC8I GCNNGC 2 cut(s) 10, 588
BstDEI CTNAG 3 cut(s) 621, 908, 951
BstDSI CCRYGG 1 cut(s) 999
BstEII GGTNACC 1 cut(s) 550
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 2 cut(s) 148, 257
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 2 cut(s) 216, 699
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 2 cut(s) 213, 696
BstMWI GCNNNNNNNGC 1 cut(s) 737
BstNI CCWGG 1 cut(s) 776
BstNSI RCATGY 1 cut(s) 476
BstPI GGTNACC 1 cut(s) 550
BstSCI CCNGG 1 cut(s) 774
BstSFI CTRYAG 1 cut(s) 561
BstSLI GKGCMC 3 cut(s) 113, 742, 1017
BstSNI TACGTA 1 cut(s) 68
BstV2I GAAGAC 2 cut(s) 318, 882
BstZ17I GTATAC 1 cut(s) 806
BsuRI GGCC 2 cut(s) 482, 731
BtgI CCRYGG 1 cut(s) 999
BtsCI GGATG 2 cut(s) 148, 257
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 4 cut(s) 261, 597, 618, 831
Cac8I GCNNGC 2 cut(s) 10, 588
CfoI GCGC 1 cut(s) 148
Cfr13I GGNCC 1 cut(s) 729
CseI GACGC 1 cut(s) 241
Csp6I GTAC 3 cut(s) 208, 545, 616
CspCI CAANNNNNGTGG 2 cut(s) 955, 990
CviJI RGCY 9 cut(s) 413, 482, 586, 683, 731, 751, 774, 882, 965
CviKI_1 RGCY 9 cut(s) 413, 482, 586, 683, 731, 751, 774, 882, 965
CviQI GTAC 3 cut(s) 208, 545, 616
DdeI CTNAG 3 cut(s) 621, 908, 951
DpnI GATC 2 cut(s) 215, 698
DpnII GATC 2 cut(s) 213, 696
EaeI YGGCCR 1 cut(s) 480
Eco105I TACGTA 1 cut(s) 68
Eco130I CCWWGG 3 cut(s) 507, 732, 999
Eco31I GGTCTC 1 cut(s) 51
Eco32I GATATC 1 cut(s) 846
Eco57I CTGAAG 1 cut(s) 876
Eco91I GGTNACC 1 cut(s) 550
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoO65I GGTNACC 1 cut(s) 550
EcoRI GAATTC 2 cut(s) 298, 896
EcoRII CCWGG 1 cut(s) 774
EcoRV GATATC 1 cut(s) 846
EcoT14I CCWWGG 3 cut(s) 507, 732, 999
EcoT22I ATGCAT 1 cut(s) 705
ErhI CCWWGG 3 cut(s) 507, 732, 999
FaqI GGGAC 1 cut(s) 348
FbaI TGATCA 1 cut(s) 696
FblI GTMKAC 2 cut(s) 805, 1005
FokI GGATG 2 cut(s) 135, 244
FspBI CTAG 2 cut(s) 239, 962
GlaI GCGC 1 cut(s) 147
HaeIII GGCC 2 cut(s) 482, 731
HgaI GACGC 1 cut(s) 241
HhaI GCGC 1 cut(s) 148
Hin1I GRCGYC 1 cut(s) 252
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HindIII AAGCTT 2 cut(s) 749, 880
HinfI GANTC 2 cut(s) 503, 655
HphI GGTGA 1 cut(s) 562
Hpy166II GTNNAC 4 cut(s) 618, 740, 806, 1006
Hpy188I TCNGA 4 cut(s) 121, 304, 895, 909
Hpy8I GTNNAC 4 cut(s) 618, 740, 806, 1006
HpyAV CCTTC 2 cut(s) 28, 934
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4IV ACGT 3 cut(s) 67, 172, 206
HpyF10VI GCNNNNNNNGC 1 cut(s) 737
HpyF3I CTNAG 3 cut(s) 621, 908, 951
HpySE526I ACGT 3 cut(s) 67, 172, 206
Hsp92I GRCGYC 1 cut(s) 252
HspAI GCGC 1 cut(s) 146
Ksp22I TGATCA 1 cut(s) 696
Kzo9I GATC 2 cut(s) 213, 696
LmnI GCTCC 1 cut(s) 583
LpnPI CCDG 7 cut(s) 116, 165, 533, 572, 712, 761, 788
LweI GCATC 1 cut(s) 157
MaeI CTAG 2 cut(s) 239, 962
MaeII ACGT 3 cut(s) 67, 172, 206
MalI GATC 2 cut(s) 215, 698
MboI GATC 2 cut(s) 213, 696
MboII GAAGA 2 cut(s) 323, 882
MfeI CAATTG 3 cut(s) 279, 401, 972
MhlI GDGCHC 3 cut(s) 113, 742, 1017
MlsI TGGCCA 1 cut(s) 482
MluCI AATT 9 cut(s) 279, 298, 338, 378, 401, 605, 781, 896, 972
MluNI TGGCCA 1 cut(s) 482
MlyI GAGTC 1 cut(s) 512
MmeI TCCRAC 1 cut(s) 206
Mox20I TGGCCA 1 cut(s) 482
Mph1103I ATGCAT 1 cut(s) 705
MroXI GAANNNNTTC 1 cut(s) 659
MscI TGGCCA 1 cut(s) 482
MseI TTAA 5 cut(s) 27, 332, 447, 465, 753
MslI CAYNNNNRTG 4 cut(s) 87, 191, 389, 698
Msp20I TGGCCA 1 cut(s) 482
MspCI CTTAAG 1 cut(s) 752
MspR9I CCNGG 1 cut(s) 776
MunI CAATTG 3 cut(s) 279, 401, 972
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 1 cut(s) 776
MwoI GCNNNNNNNGC 1 cut(s) 737
NcoI CCATGG 1 cut(s) 999
NdeII GATC 2 cut(s) 213, 696
NlaIV GGNNCC 1 cut(s) 110
NmuCI GTSAC 4 cut(s) 253, 382, 550, 868
NsiI ATGCAT 1 cut(s) 705
NspI RCATGY 1 cut(s) 476
PctI GAATGC 1 cut(s) 10
PdmI GAANNNNTTC 1 cut(s) 659
PfeI GAWTC 1 cut(s) 655
PflFI GACNNNGTC 1 cut(s) 89
PleI GAGTC 1 cut(s) 511
PpsI GAGTC 1 cut(s) 511
Ppu21I YACGTR 3 cut(s) 68, 173, 207
PshBI ATTAAT 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 774
PspEI GGTNACC 1 cut(s) 550
PspGI CCWGG 1 cut(s) 774
PspN4I GGNNCC 1 cut(s) 110
PspPI GGNCC 1 cut(s) 729
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 3 cut(s) 209, 546, 617
RsaNI GTAC 3 cut(s) 208, 545, 616
RseI CAYNNNNRTG 4 cut(s) 87, 191, 389, 698
SaqAI TTAA 5 cut(s) 27, 332, 447, 465, 753
Sau3AI GATC 2 cut(s) 213, 696
Sau96I GGNCC 1 cut(s) 729
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 1 cut(s) 776
SduI GDGCHC 3 cut(s) 113, 742, 1017
SfaNI GCATC 1 cut(s) 157
SfcI CTRYAG 1 cut(s) 561
SmiMI CAYNNNNRTG 4 cut(s) 87, 191, 389, 698
SmlI CTYRAG 1 cut(s) 752
SmoI CTYRAG 1 cut(s) 752
SnaBI TACGTA 1 cut(s) 68
Sse9I AATT 9 cut(s) 279, 298, 338, 378, 401, 605, 781, 896, 972
SspMI CTAG 2 cut(s) 239, 962
StyD4I CCNGG 1 cut(s) 774
StyI CCWWGG 3 cut(s) 507, 732, 999
TaaI ACNGT 1 cut(s) 63
TaiI ACGT 3 cut(s) 70, 175, 209
TaqI TCGA 1 cut(s) 93
TasI AATT 9 cut(s) 279, 298, 338, 378, 401, 605, 781, 896, 972
TatI WGTACW 2 cut(s) 544, 615
TfiI GAWTC 1 cut(s) 655
Tru1I TTAA 5 cut(s) 27, 332, 447, 465, 753
Tru9I TTAA 5 cut(s) 27, 332, 447, 465, 753
TscAI CASTG 4 cut(s) 268, 597, 625, 838
TseFI GTSAC 4 cut(s) 253, 382, 550, 868
Tsp45I GTSAC 4 cut(s) 253, 382, 550, 868
TspDTI ATGAA 2 cut(s) 17, 548
TspGWI ACGGA 1 cut(s) 704
TspRI CASTG 4 cut(s) 268, 597, 625, 838
Tth111I GACNNNGTC 1 cut(s) 89
Vha464I CTTAAG 1 cut(s) 752
VneI GTGCAC 1 cut(s) 738
VspI ATTAAT 1 cut(s) 465
XagI CCTNNNNNAGG 1 cut(s) 239
XapI RAATTY 4 cut(s) 298, 605, 781, 896
XceI RCATGY 1 cut(s) 476
XmiI GTMKAC 2 cut(s) 805, 1005
XmnI GAANNNNTTC 1 cut(s) 659
XspI CTAG 2 cut(s) 239, 962
Zsp2I ATGCAT 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.