pycom10g02110

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000143
Physical Location & Seq
Forward (+)
2240009 .. 2242905
2897 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g02110.4

Sequence Viewer

Length: 2544 bp
ATGGATTTCACAAGTGAATTTACAACTTATGTGATATTCAAGACTAGAGAGTCCTTGATGCAGTGGGCTCGTGAGGCTGGAAAGCGAAATGGGTTTGTTATTGTGACAATACGATCTGATATGGGTAGTAAGAGTACAAAACCAAGAGTTACATTAGGTTGCGAGAGGGGTGGAAGATTTAAGGAGAAGAAAACTAGGGCTGCAAAAAAAAGACGTGGGACAGGTACAAAGAAGTGTGGGTGTCCTTTCACATTGAAAGGGGAGAAGTCAGCGAATGGGGACGGATGGATGCTAAAGGTTGTATGTGGAGTGCATAACCATGCATCCACCGAGTATCCTGATGGGCGATCATATGCAGGGAGGTTATCTGAGCAGGAGACTTCACTCTTGATAGACTTGTCAAAGAGTTTAGTACGACCCAAAGAAATCCTGAGCACATTGAAGCAAAGGGATGAACTCAACGCTACAACCATAAGGACGATCTATAATGCACGCCAAAGGCAAAGAGTTAAAGAGAAGGCAGGGAGGTCTCAAATGCAACAACTGTTATGTAAGTTGAATGAGCATGACTATGTCGAGTGGCATAGGAGGTGCCCTATGTCAGATAGTGTGCAGGACTTGTTTTGGGCGCACCCTGTGAGTTTGGAGTTGTTACGTGCATTTCCAACCATTTTGATGATAGATTGCACGTATAAGACCAATAGGTACCATTATCCCTTACTAGAGGTTGTAGGCGTCACATCCACTGATATTACATTCTCAGTTGCATTTGTCTTTTTGGATTCTGAAAAAGAAGACAACTATATATGGGCATTAAGTAAATTGCGTGGTGTCATGGTGGAGTGTCCAATGCCAAATGTAATTGTCACAGACATGGATTTGCCATTGATGAGAGCCATTGAGGTAGTATTCCCTACAGCTAAAAATCTTTTATGTAAATGGCATATTAATAAGCATGTGTTGGCAAAATGCAAAAACTTGTTTGAGTCCAAGGAAAGGTGTGATAGATTTATTATGAAGTGGAACGTACTTGTGAGCTCTCCTACAGAAAATGATTACATACGGGATCTAGCAGTGTTACAGAGAGAATTTAGTATGTACGCTGAGGTCATGGAGTATGTTACAAACACATGGTTGAATCCTTTCAAGGACAGATTTGTGTCGGCTTGGACAGACATCGATCATGCATTTTGGTAAGTAACATCAAACAGGGCCGAGGGTGTACATACCAAGCTTAAGAGGGAGCTTGGTATGAGCCAGGGGAATTTTGAGGGTTCTTTTGAGAGTATACACTCTCTTATTGAGTTACAGCACACTGACATAAAGATATCTTTTGACAAGTGTTTGACGGTCACACAACAAAGCTTCAAGTCTTCAGAATTCAAATATTTGAGAGGAGTTGTATCTATGTTTGCATTGGAGAAGGTGTTATCTCAGTCCAAACTAGCCATCTCAATTGGTGCTGGTGGTTTAGGATGTGGTTGTGTCATACCCAGAACGCATGGTCTACCTTGTGCCCACGAGATTGTGAAATACAAGAGGGAGTGTCGGCCAATTCCTCTTGAGTCGCTTGACCCCCATTGGACTAAACTTGATATACTACCTCCGAGTTCTGCTCAGGAATCTATGGATGTGAATGAGTTTCCTGAGTTGGAGTTGCTTCAACGATACTTTCTGGAGGCAAATGCAGAAAATAGGTTGATGCTCAGGAAAAGAGTGAGAGAACTTGTAGCACCCTACACAACTAGTCTTGTTGAACCAGGAGTCAAGTCCAGATTACGAGGACAACTGACGGAGAAAATTGATATGCCAACTCAACAGGACCCATATGCATTTGAGTTGAAATCTGCATCTGTGTATAGTCACTCACCTAGCGCAATGGATGATCAGCAAAATTTTAAACATGAGCAAACGGAGCGAACCAAGGAGGTGTACCGGACATTATCAATGAAAGGTATTTCGTATATTGATGCGTTCCCAATTGGATTGAGGCCCTTCATTCAGCAAGTCAAGGATGTACGTCATGATGGTCACTGTGGGTTTAGGGCAGTTGCTGATTTGGTGGGATTGGGTGAGAATGGGTACCTGCAAGTTCGTAATGACCTGCTTACTGAGTTGTCTTCATATTCTGCACATTATGGCGAGTTGTATGGCAGCACAGACAGGGTTCGTGAGTTAGCACAGATACTATCATTCTTTGATGAAGGTAGAGCACCATTTGACAGATGGATGATGATGCCTGACATGGGTCATCTTATAGCATCATGCTATAAGGTTGTGCTTTTCCACTTATCATCTACTCAGTGCCTTACCTTCCTACCACTTCGGTCAGCACCAGTTTCTTTGCCAAGGCGTCGACAAATTGCTATTGGTTTTATGAATGATTGTCATTGTGTGGAGGTGTTCTTAAGGCCGGGGCATCCTGTACCTCCCATTGCGTCTGATTGGATCAGATATCATACGCCAGCTGCACAAGGATGGGACACTGTGTATACTAGTTGTATTTTACGCTTCAAATCTCTTGTTAGCTGTGATCAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
FAR1 MULE SET

Protein Analysis

848

Amino Acids

96.9

Weight (kDa)

8.77

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76710 AT1G76710 AT1G76710 AT1G76710
fragaria_vesca FvH4_1g20063 FvH4_2g15231 FvH4_3g04011 FvH4_3g28681 FvH4_4g01411 FvH4_4g10181 FvH4_4g15922 FvH4_4g16651 FvH4_4g16652 FvH4_5g13892 FvH4_5g13893 FvH4_5g20623 FvH4_5g20623 FvH4_5g20623 FvH4_5g20623 FvH4_5g25511 FvH4_6g12551 FvH4_6g12552 FvH4_6g38841 FvH4_7g04391
malus_domestica MD05G1031300.v1.1 MD05G1031400.v1.1 MD05G1031500.v1.1 MD08G1164400.v1.1 MD10G1032900.v1.1 MD10G1033000.v1.1 MD10G1033100.v1.1
prunus_persica Prupe.3G240700_v2.0.a1 Prupe.7G144600_v2.0.a1 Prupe.8G038500_v2.0.a1 Prupe.8G038500_v2.0.a1
pyrus_communis pycom05g02250 pycom05g02260 pycom05g02270 pycom05g02280 pycom08g14160 pycom10g02100 pycom10g02110 pycom10g02130
rosa_chinensis RchiOBHm_Chr1g0329481 RchiOBHm_Chr1g0329491 RchiOBHm_Chr1g0341531 RchiOBHm_Chr3g0464811 RchiOBHm_Chr3g0494701 RchiOBHm_Chr3g0497501 RchiOBHm_Chr4g0385841 RchiOBHm_Chr5g0061181 RchiOBHm_Chr6g0263001 RchiOBHm_Chr7g0193111 RchiOBHm_Chr7g0206281 RchiOBHm_Chr7g0206311
rosa_laevigata RLG00000018908 RLG00000028528 RLG00000028803 RLG00000029882 RLG00000029883 RLG00000030361
rosa_multiflora Rmu_co8196328.1_g000001 Rmu_co8259135.1_g000001 Rmu_co8269185.1_g000001 Rmu_sc0000076.1_g000019 Rmu_sc0000311.1_g000007 Rmu_sc0000429.1_g000038 Rmu_sc0000429.1_g000039 Rmu_sc0000429.1_g000040 Rmu_sc0000511.1_g000005 Rmu_sc0000539.1_g000054 Rmu_sc0000566.1_g000039 Rmu_sc0000805.1_g000032 Rmu_sc0000870.1_g000073 Rmu_sc0000870.1_g000074 Rmu_sc0001010.1_g000018 Rmu_sc0001083.1_g000013 Rmu_sc0001114.1_g000011 Rmu_sc0001702.1_g000027 Rmu_sc0001779.1_g000012 Rmu_sc0002923.1_g000036 Rmu_sc0003242.1_g000011 Rmu_sc0003291.1_g000036 Rmu_sc0003393.1_g000012 Rmu_sc0003872.1_g000016 Rmu_sc0003872.1_g000017 Rmu_sc0003872.1_g000018 Rmu_sc0004084.1_g000020 Rmu_sc0004386.1_g000005 Rmu_sc0004622.1_g000005 Rmu_sc0004742.1_g000021 Rmu_sc0004755.1_g000020 Rmu_sc0004805.1_g000036 Rmu_sc0005065.1_g000006 Rmu_sc0005177.1_g000004 Rmu_sc0006373.1_g000003 Rmu_sc0006373.1_g000004 Rmu_sc0007121.1_g000002 Rmu_sc0007943.1_g000003 Rmu_sc0010368.1_g000006 Rmu_sc0010543.1_g000001 Rmu_sc0012591.1_g000006 Rmu_sc0013504.1_g000009 Rmu_sc0015025.1_g000001 Rmu_sc0016170.1_g000001 Rmu_sc0017156.1_g000001 Rmu_sc0026439.1_g000001
rosa_roxburghii Rroxscaffold_175G00432280 Rroxscaffold_1G00030190 Rroxscaffold_1G00030880 Rroxscaffold_2G00090520 Rroxscaffold_3G00227940 Rroxscaffold_3G00233400 Rroxscaffold_3G00238500 Rroxscaffold_3G00251650 Rroxscaffold_4G00301570 Rroxscaffold_4G00321040 Rroxscaffold_5G00350880 Rroxscaffold_7G00184400
rosa_rugosa Rorug01G0080800 Rorug01G0080900 Rorug01G0193900 Rorug02G0275900 Rorug02G0275900 Rorug02G0275900 Rorug02G0305400 Rorug02G0311800 Rorug02G0350400 Rorug02G0381300 Rorug03G0136700 Rorug03G0181900 Rorug03G0268000 Rorug04G0301600 Rorug05G0175100 Rorug05G0250700 Rorug05G0298200 Rorug05G0596000 Rorug06G0029500 Rorug07G0094300 Rorug07G0094300 Rorug07G0095400 Rorug07G0140100
rosa_samantha Rh1AG099200 Rh1AG190500 Rh1BG078900 Rh1BG079000 Rh1BG079100 Rh1CG084600 Rh1CG084700 Rh1CG095300 Rh1CG175900 Rh1DG103300 Rh1DG150400 Rh2AG079300 Rh2AG272400 Rh2AG464800 Rh2AG507400 Rh2DG078300 Rh2DG078400 Rh3CG206900 Rh3CG295400 Rh3DG206800 Rh3DG356200 Rh4CG071500 Rh4CG179200 Rh4DG064700 Rh5CG362400 Rh7AG212300 Rh7AG226200 Rh7BG222200
rosa_wichuraiana Rw0G009990 Rw0G011260 Rw0G011270 Rw0G012910 Rw0G013740 Rw1G007740 Rw1G013050 Rw1G014230 Rw2G014270 Rw3G012720 Rw3G028110 Rw4G006630 Rw4G015270 Rw4G016160 Rw4G031380 Rw5G011440 Rw5G029180 Rw5G048360 Rw5G048430 Rw5G048780 Rw6G005250 Rw6G006390 Rw7G002840 Rw7G014080 Rw7G019650 Rw7G032790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 49
Acc36I ACCTGC 2 cut(s) 2094, 2112
Acc65I GGTACC 2 cut(s) 705, 2082
AccB1I GGYRCC 3 cut(s) 591, 705, 2082
AccI GTMKAC 4 cut(s) 1288, 1507, 2356, 2492
AclWI GGATC 2 cut(s) 1074, 2456
AcoI YGGCCR 1 cut(s) 1550
AcsI RAATTY 5 cut(s) 17, 1088, 1264, 1379, 1894
AcuI CTGAAG 1 cut(s) 1359
AcyI GRCGYC 2 cut(s) 735, 2353
AdeI CACNNNGTG 1 cut(s) 637
AfiI CCNNNNNNNGG 2 cut(s) 996, 2246
AflII CTTAAG 2 cut(s) 1235, 2407
AhlI ACTAGT 2 cut(s) 1745, 2495
AjiI CACGTC 1 cut(s) 215
AjnI CCWGG 2 cut(s) 1257, 1759
AluBI AGCT 7 cut(s) 920, 1038, 1234, 1246, 1365, 2468, 2529
AluI AGCT 7 cut(s) 920, 1038, 1234, 1246, 1365, 2468, 2529
Alw21I GWGCWC 3 cut(s) 437, 1040, 2215
Alw26I GTCTC 2 cut(s) 371, 534
AlwI GGATC 2 cut(s) 1074, 2456
AlwNI CAGNNNCTG 1 cut(s) 2054
AoxI GGCC 4 cut(s) 1212, 1550, 1991, 2411
ApeKI GCWGC 3 cut(s) 200, 2154, 2468
ApoI RAATTY 5 cut(s) 17, 1088, 1264, 1379, 1894
AseI ATTAAT 1 cut(s) 948
Asp700I GAANNNNTTC 1 cut(s) 1142
Asp718I GGTACC 2 cut(s) 705, 2082
AspLEI GCGC 2 cut(s) 631, 1877
AspS9I GGNCC 3 cut(s) 1212, 1822, 1992
AsuC2I CCSGG 1 cut(s) 2415
AsuHPI GGTGA 2 cut(s) 1860, 2084
AvaII GGWCC 1 cut(s) 1822
BaeGI GKGCMC 2 cut(s) 596, 1519
BanI GGYRCC 3 cut(s) 591, 705, 2082
BanII GRGCYC 2 cut(s) 70, 1040
BauI CACGAG 2 cut(s) 69, 1520
BbsI GAAGAC 3 cut(s) 801, 1365, 2112
Bbv12I GWGCWC 3 cut(s) 437, 1040, 2215
BbvCI CCTCAGC 1 cut(s) 1104
BbvI GCAGC 3 cut(s) 187, 2166, 2455
BccI CCATC 6 cut(s) 279, 335, 1457, 2021, 2220, 2472
BcgI CGANNNNNNTGC 2 cut(s) 50, 84
BciT130I CCWGG 2 cut(s) 1259, 1761
BciVI GTATCC 1 cut(s) 345
BclI TGATCA 2 cut(s) 1885, 2533
BcnI CCSGG 1 cut(s) 2415
BcoDI GTCTC 2 cut(s) 371, 534
BcuI ACTAGT 2 cut(s) 1745, 2495
BfaI CTAG 8 cut(s) 45, 195, 722, 1070, 1445, 1746, 1872, 2496
BfmI CTRYAG 2 cut(s) 915, 1044
BfrI CTTAAG 2 cut(s) 1235, 2407
BfuAI ACCTGC 2 cut(s) 2094, 2112
BfuI GTATCC 1 cut(s) 345
BisI GCNGC 3 cut(s) 201, 2155, 2469
BlsI GCNGC 3 cut(s) 202, 2156, 2470
Bme1390I CCNGG 3 cut(s) 1259, 1761, 2415
Bme18I GGWCC 1 cut(s) 1822
BmgBI CACGTC 1 cut(s) 215
BmgT120I GGNCC 3 cut(s) 1212, 1822, 1992
BmiI GGNNCC 4 cut(s) 593, 707, 1824, 2084
BmrFI CCNGG 3 cut(s) 1259, 1761, 2415
BmsI GCATC 9 cut(s) 48, 279, 332, 1692, 1859, 1960, 2226, 2270, 2428
BoxI GACNNNNGTC 1 cut(s) 2247
BpiI GAAGAC 3 cut(s) 801, 1365, 2112
BplI GAGNNNNNCTC 2 cut(s) 1600, 1632
BpmI CTGGAG 1 cut(s) 1697
Bpu10I CCTNAGC 4 cut(s) 431, 1104, 1617, 1706
BpuEI CTTGAG 1 cut(s) 1583
BpuMI CCSGG 1 cut(s) 2415
Bsa29I ATCGAT 1 cut(s) 1179
BsaAI YACGTR 2 cut(s) 656, 690
BsaHI GRCGYC 2 cut(s) 735, 2353
BsaI GGTCTC 1 cut(s) 534
BsaJI CCNNGG 6 cut(s) 990, 1215, 1258, 1923, 2348, 2414
BsaWI WCCGGW 1 cut(s) 1935
BsaXI ACNNNNNCTCC 2 cut(s) 1412, 1442
Bsc4I CCNNNNNNNGG 2 cut(s) 996, 2246
Bse1I ACTGG 1 cut(s) 2336
Bse3DI GCAATG 2 cut(s) 1884, 2433
BseBI CCWGG 2 cut(s) 1259, 1761
BseCI ATCGAT 1 cut(s) 1179
BseDI CCNNGG 6 cut(s) 990, 1215, 1258, 1923, 2348, 2414
BseLI CCNNNNNNNGG 2 cut(s) 996, 2246
BseMI GCAATG 2 cut(s) 1884, 2433
BseNI ACTGG 1 cut(s) 2336
BseRI GAGGAG 1 cut(s) 1410
BseSI GKGCMC 2 cut(s) 596, 1519
BseXI GCAGC 3 cut(s) 187, 2166, 2455
BsgI GTGCAG 3 cut(s) 632, 2115, 2454
BshFI GGCC 4 cut(s) 1214, 1552, 1993, 2413
BshNI GGYRCC 3 cut(s) 591, 705, 2082
BshVI ATCGAT 1 cut(s) 1179
BsiHKAI GWGCWC 3 cut(s) 437, 1040, 2215
BsiSI CCGG 2 cut(s) 1936, 2414
BslFI GGGAC 3 cut(s) 232, 293, 2495
BslI CCNNNNNNNGG 2 cut(s) 996, 2246
BsmAI GTCTC 2 cut(s) 371, 534
BsmFI GGGAC 3 cut(s) 232, 293, 2495
BsnI GGCC 4 cut(s) 1214, 1552, 1993, 2413
Bso31I GGTCTC 1 cut(s) 534
Bsp1286I GDGCHC 6 cut(s) 70, 437, 596, 1040, 1519, 2215
Bsp1407I TGTACA 1 cut(s) 1222
Bsp143I GATC 8 cut(s) 113, 347, 480, 1066, 1180, 1885, 2448, 2533
BspANI GGCC 4 cut(s) 1214, 1552, 1993, 2413
BspDI ATCGAT 1 cut(s) 1179
BspHI TCATGA 1 cut(s) 2023
BspLI GGNNCC 4 cut(s) 593, 707, 1824, 2084
BspMI ACCTGC 2 cut(s) 2094, 2112
BspPI GGATC 2 cut(s) 1074, 2456
BspT107I GGYRCC 3 cut(s) 591, 705, 2082
BspTI CTTAAG 2 cut(s) 1235, 2407
BspTNI GGTCTC 1 cut(s) 534
BsrDI GCAATG 2 cut(s) 1884, 2433
BsrGI TGTACA 1 cut(s) 1222
BsrI ACTGG 1 cut(s) 2336
BssECI CCNNGG 6 cut(s) 990, 1215, 1258, 1923, 2348, 2414
BssMI GATC 8 cut(s) 113, 347, 480, 1066, 1180, 1885, 2448, 2533
BssNAI GTATAC 2 cut(s) 1289, 2493
BssNI GRCGYC 2 cut(s) 735, 2353
BssSI CACGAG 2 cut(s) 69, 1520
BssT1I CCWWGG 3 cut(s) 990, 1923, 2348
Bst1107I GTATAC 2 cut(s) 1289, 2493
Bst2BI CACGAG 2 cut(s) 69, 1520
Bst2UI CCWGG 2 cut(s) 1259, 1761
Bst4CI ACNGT 4 cut(s) 546, 1351, 2036, 2488
BstACI GRCGYC 2 cut(s) 735, 2353
BstAFI CTTAAG 2 cut(s) 1235, 2407
BstAUI TGTACA 1 cut(s) 1222
BstBAI YACGTR 2 cut(s) 656, 690
BstC8I GCNNGC 2 cut(s) 493, 2466
BstHHI GCGC 2 cut(s) 631, 1877
BstKTI GATC 8 cut(s) 116, 350, 483, 1069, 1183, 1888, 2451, 2536
BstMAI GTCTC 2 cut(s) 371, 534
BstMBI GATC 8 cut(s) 113, 347, 480, 1066, 1180, 1885, 2448, 2533
BstMWI GCNNNNNNNGC 2 cut(s) 74, 1915
BstNI CCWGG 2 cut(s) 1259, 1761
BstNSI RCATGY 1 cut(s) 959
BstPAI GACNNNNGTC 1 cut(s) 2247
BstSCI CCNGG 3 cut(s) 1257, 1759, 2413
BstSFI CTRYAG 2 cut(s) 915, 1044
BstSLI GKGCMC 2 cut(s) 596, 1519
BstV1I GCAGC 3 cut(s) 187, 2166, 2455
BstV2I GAAGAC 3 cut(s) 801, 1365, 2112
BstX2I RGATCY 1 cut(s) 1066
BstYI RGATCY 1 cut(s) 1066
BstZ17I GTATAC 2 cut(s) 1289, 2493
Bsu15I ATCGAT 1 cut(s) 1179
BsuI GTATCC 1 cut(s) 345
BsuRI GGCC 4 cut(s) 1214, 1552, 1993, 2413
BsuTUI ATCGAT 1 cut(s) 1179
BtrI CACGTC 1 cut(s) 215
BtsI GCAGTG 2 cut(s) 68, 1080
BtsIMutI CAGTG 7 cut(s) 68, 744, 1080, 1314, 2032, 2309, 2484
BveI ACCTGC 2 cut(s) 2094, 2112
Cac8I GCNNGC 2 cut(s) 493, 2466
CaiI CAGNNNCTG 1 cut(s) 2054
CciI TCATGA 1 cut(s) 2023
CfoI GCGC 2 cut(s) 631, 1877
Cfr13I GGNCC 3 cut(s) 1212, 1822, 1992
ClaI ATCGAT 1 cut(s) 1179
CseI GACGC 3 cut(s) 724, 2342, 2427
DpnI GATC 8 cut(s) 115, 349, 482, 1068, 1182, 1887, 2450, 2535
DpnII GATC 8 cut(s) 113, 347, 480, 1066, 1180, 1885, 2448, 2533
DraI TTTAAA 1 cut(s) 1900
DraIII CACNNNGTG 1 cut(s) 637
DrdI GACNNNNNNGTC 1 cut(s) 49
DseDI GACNNNNNNGTC 1 cut(s) 49
EaeI YGGCCR 1 cut(s) 1550
Ecl136II GAGCTC 1 cut(s) 1038
Eco130I CCWWGG 3 cut(s) 990, 1923, 2348
Eco24I GRGCYC 2 cut(s) 70, 1040
Eco31I GGTCTC 1 cut(s) 534
Eco32I GATATC 2 cut(s) 1329, 2456
Eco47I GGWCC 1 cut(s) 1822
Eco53kI GAGCTC 1 cut(s) 1038
Eco57I CTGAAG 1 cut(s) 1359
EcoICRI GAGCTC 1 cut(s) 1038
EcoO109I RGGNCCY 2 cut(s) 1822, 1992
EcoRI GAATTC 1 cut(s) 1379
EcoRII CCWGG 2 cut(s) 1257, 1759
EcoRV GATATC 2 cut(s) 1329, 2456
EcoT14I CCWWGG 3 cut(s) 990, 1923, 2348
EcoT22I ATGCAT 3 cut(s) 325, 1189, 1834
EcoT38I GRGCYC 2 cut(s) 70, 1040
ErhI CCWWGG 3 cut(s) 990, 1923, 2348
FaqI GGGAC 3 cut(s) 232, 293, 2495
FauNDI CATATG 2 cut(s) 352, 1828
FbaI TGATCA 2 cut(s) 1885, 2533
FblI GTMKAC 4 cut(s) 1288, 1507, 2356, 2492
Fnu4HI GCNGC 3 cut(s) 201, 2155, 2469
FriOI GRGCYC 2 cut(s) 70, 1040
Fsp4HI GCNGC 3 cut(s) 201, 2155, 2469
FspBI CTAG 8 cut(s) 45, 195, 722, 1070, 1445, 1746, 1872, 2496
GlaI GCGC 2 cut(s) 630, 1876
GluI GCNGC 3 cut(s) 201, 2155, 2469
GsuI CTGGAG 1 cut(s) 1697
HaeIII GGCC 4 cut(s) 1214, 1552, 1993, 2413
HapII CCGG 2 cut(s) 1936, 2414
HgaI GACGC 3 cut(s) 724, 2342, 2427
HhaI GCGC 2 cut(s) 631, 1877
Hin1I GRCGYC 2 cut(s) 735, 2353
Hin6I GCGC 2 cut(s) 629, 1875
HinP1I GCGC 2 cut(s) 629, 1875
HincII GTYRAC 1 cut(s) 2357
HindII GTYRAC 1 cut(s) 2357
HindIII AAGCTT 2 cut(s) 1232, 1363
HinfI GANTC 7 cut(s) 50, 782, 986, 1138, 1565, 1622, 1764
HpaII CCGG 2 cut(s) 1936, 2414
HphI GGTGA 2 cut(s) 1860, 2084
Hpy166II GTNNAC 6 cut(s) 1223, 1289, 1508, 1933, 2357, 2493
Hpy188I TCNGA 9 cut(s) 118, 370, 604, 787, 1378, 1608, 2443, 2453, 2543
Hpy8I GTNNAC 6 cut(s) 1223, 1289, 1508, 1933, 2357, 2493
Hpy99I CGWCG 1 cut(s) 2358
HpyAV CCTTC 5 cut(s) 511, 1417, 2005, 2198, 2323
HpyCH4III ACNGT 4 cut(s) 546, 1351, 2036, 2488
HpyCH4IV ACGT 5 cut(s) 214, 655, 689, 1026, 2020
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 1915
HpySE526I ACGT 5 cut(s) 214, 655, 689, 1026, 2020
Hsp92I GRCGYC 2 cut(s) 735, 2353
HspAI GCGC 2 cut(s) 629, 1875
KpnI GGTACC 2 cut(s) 709, 2086
Ksp22I TGATCA 2 cut(s) 1885, 2533
Kzo9I GATC 8 cut(s) 113, 347, 480, 1066, 1180, 1885, 2448, 2533
LmnI GCTCC 2 cut(s) 1243, 1915
Lsp1109I GCAGC 3 cut(s) 187, 2166, 2455
LweI GCATC 9 cut(s) 48, 279, 332, 1692, 1859, 1960, 2226, 2270, 2428
MaeI CTAG 8 cut(s) 45, 195, 722, 1070, 1445, 1746, 1872, 2496
MaeII ACGT 5 cut(s) 214, 655, 689, 1026, 2020
MalI GATC 8 cut(s) 115, 349, 482, 1068, 1182, 1887, 2450, 2535
MboI GATC 8 cut(s) 113, 347, 480, 1066, 1180, 1885, 2448, 2533
MboII GAAGA 5 cut(s) 186, 199, 806, 1365, 2112
MfeI CAATTG 2 cut(s) 1455, 1980
MflI RGATCY 1 cut(s) 1066
MhlI GDGCHC 6 cut(s) 70, 437, 596, 1040, 1519, 2215
MlyI GAGTC 4 cut(s) 59, 995, 1574, 1773
MmeI TCCRAC 2 cut(s) 689, 1632
Mph1103I ATGCAT 3 cut(s) 325, 1189, 1834
MroXI GAANNNNTTC 1 cut(s) 1142
MseI TTAA 7 cut(s) 180, 510, 815, 948, 1236, 1899, 2408
MslI CAYNNNNRTG 5 cut(s) 318, 570, 674, 872, 2476
MspA1I CMGCKG 1 cut(s) 2468
MspCI CTTAAG 2 cut(s) 1235, 2407
MspI CCGG 2 cut(s) 1936, 2414
MspR9I CCNGG 3 cut(s) 1259, 1761, 2415
MunI CAATTG 2 cut(s) 1455, 1980
MvaI CCWGG 2 cut(s) 1259, 1761
MwoI GCNNNNNNNGC 2 cut(s) 74, 1915
NciI CCSGG 1 cut(s) 2415
NdeI CATATG 2 cut(s) 352, 1828
NdeII GATC 8 cut(s) 113, 347, 480, 1066, 1180, 1885, 2448, 2533
NlaIV GGNNCC 4 cut(s) 593, 707, 1824, 2084
NmeAIII GCCGAG 1 cut(s) 1240
NmuCI GTSAC 6 cut(s) 103, 736, 865, 1351, 1862, 2030
NsiI ATGCAT 3 cut(s) 325, 1189, 1834
NspI RCATGY 1 cut(s) 959
PagI TCATGA 1 cut(s) 2023
PdmI GAANNNNTTC 1 cut(s) 1142
PfeI GAWTC 3 cut(s) 782, 1138, 1622
PflFI GACNNNGTC 1 cut(s) 572
PkrI GCNGC 3 cut(s) 202, 2156, 2470
PleI GAGTC 4 cut(s) 58, 994, 1573, 1772
PpsI GAGTC 4 cut(s) 58, 994, 1573, 1772
Ppu21I YACGTR 2 cut(s) 656, 690
PpuMI RGGWCCY 1 cut(s) 1822
PshAI GACNNNNGTC 1 cut(s) 2247
PshBI ATTAAT 1 cut(s) 948
Psp124BI GAGCTC 1 cut(s) 1040
Psp5II RGGWCCY 1 cut(s) 1822
Psp6I CCWGG 2 cut(s) 1257, 1759
PspGI CCWGG 2 cut(s) 1257, 1759
PspN4I GGNNCC 4 cut(s) 593, 707, 1824, 2084
PspPI GGNCC 3 cut(s) 1212, 1822, 1992
PspPPI RGGWCCY 1 cut(s) 1822
PstNI CAGNNNCTG 1 cut(s) 2054
PsuI RGATCY 1 cut(s) 1066
PsyI GACNNNGTC 1 cut(s) 572
PvuII CAGCTG 1 cut(s) 2468
RseI CAYNNNNRTG 5 cut(s) 318, 570, 674, 872, 2476
SacI GAGCTC 1 cut(s) 1040
SalI GTCGAC 1 cut(s) 2355
SaqAI TTAA 7 cut(s) 180, 510, 815, 948, 1236, 1899, 2408
SatI GCNGC 3 cut(s) 201, 2155, 2469
Sau3AI GATC 8 cut(s) 113, 347, 480, 1066, 1180, 1885, 2448, 2533
Sau96I GGNCC 3 cut(s) 1212, 1822, 1992
SchI GAGTC 4 cut(s) 59, 995, 1574, 1773
ScrFI CCNGG 3 cut(s) 1259, 1761, 2415
SduI GDGCHC 6 cut(s) 70, 437, 596, 1040, 1519, 2215
SfaNI GCATC 9 cut(s) 48, 279, 332, 1692, 1859, 1960, 2226, 2270, 2428
SfcI CTRYAG 2 cut(s) 915, 1044
SinI GGWCC 1 cut(s) 1822
SmiMI CAYNNNNRTG 5 cut(s) 318, 570, 674, 872, 2476
SmlI CTYRAG 3 cut(s) 1235, 1562, 2407
SmoI CTYRAG 3 cut(s) 1235, 1562, 2407
SpeI ACTAGT 2 cut(s) 1745, 2495
SspI AATATT 1 cut(s) 1388
SspMI CTAG 8 cut(s) 45, 195, 722, 1070, 1445, 1746, 1872, 2496
SstI GAGCTC 1 cut(s) 1040
StyD4I CCNGG 3 cut(s) 1257, 1759, 2413
StyI CCWWGG 3 cut(s) 990, 1923, 2348
TaaI ACNGT 4 cut(s) 546, 1351, 2036, 2488
TaiI ACGT 5 cut(s) 217, 658, 692, 1029, 2023
TaqI TCGA 3 cut(s) 576, 1179, 2356
TaqII GACCGA 1 cut(s) 2316
TatI WGTACW 2 cut(s) 134, 1222
TfiI GAWTC 3 cut(s) 782, 1138, 1622
Tru1I TTAA 7 cut(s) 180, 510, 815, 948, 1236, 1899, 2408
Tru9I TTAA 7 cut(s) 180, 510, 815, 948, 1236, 1899, 2408
TscAI CASTG 7 cut(s) 68, 751, 1080, 1321, 2039, 2309, 2491
TseFI GTSAC 6 cut(s) 103, 736, 865, 1351, 1862, 2030
TseI GCWGC 3 cut(s) 200, 2154, 2468
Tsp45I GTSAC 6 cut(s) 103, 736, 865, 1351, 1862, 2030
TspDTI ATGAA 7 cut(s) 468, 1031, 1964, 1987, 2112, 2217, 2393
TspGWI ACGGA 3 cut(s) 297, 1808, 1928
TspRI CASTG 7 cut(s) 68, 751, 1080, 1321, 2039, 2309, 2491
Tth111I GACNNNGTC 1 cut(s) 572
Vha464I CTTAAG 2 cut(s) 1235, 2407
VpaK11BI GGWCC 1 cut(s) 1822
VspI ATTAAT 1 cut(s) 948
XapI RAATTY 5 cut(s) 17, 1088, 1264, 1379, 1894
XceI RCATGY 1 cut(s) 959
XcmI CCANNNNNNNNNTGG 1 cut(s) 2223
XmiI GTMKAC 4 cut(s) 1288, 1507, 2356, 2492
XmnI GAANNNNTTC 1 cut(s) 1142
XspI CTAG 8 cut(s) 45, 195, 722, 1070, 1445, 1746, 1872, 2496
Zsp2I ATGCAT 3 cut(s) 325, 1189, 1834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.