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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AluBI | AGCT | 1 cut(s) | 98 |
| AluI | AGCT | 1 cut(s) | 98 |
| BccI | CCATC | 1 cut(s) | 64 |
| Bpu10I | CCTNAGC | 1 cut(s) | 31 |
| Bse3DI | GCAATG | 1 cut(s) | 94 |
| BseMI | GCAATG | 1 cut(s) | 94 |
| BseMII | CTCAG | 1 cut(s) | 45 |
| BsmI | GAATGC | 1 cut(s) | 111 |
| BspCNI | CTCAG | 1 cut(s) | 44 |
| BsrDI | GCAATG | 1 cut(s) | 94 |
| BstDEI | CTNAG | 1 cut(s) | 31 |
| CviAII | CATG | 1 cut(s) | 27 |
| CviJI | RGCY | 2 cut(s) | 98, 141 |
| CviKI_1 | RGCY | 2 cut(s) | 98, 141 |
| DdeI | CTNAG | 1 cut(s) | 31 |
| EcoT22I | ATGCAT | 1 cut(s) | 111 |
| FaeI | CATG | 1 cut(s) | 30 |
| FaiI | YATR | 5 cut(s) | 28, 51, 111, 118, 122 |
| FatI | CATG | 1 cut(s) | 26 |
| FspEI | CC | 7 cut(s) | 18, 59, 65, 69, 112, 113, 131 |
| Hin1II | CATG | 1 cut(s) | 30 |
| Hpy188I | TCNGA | 1 cut(s) | 135 |
| Hpy188III | TCNNGA | 1 cut(s) | 60 |
| HpyCH4V | TGCA | 2 cut(s) | 92, 109 |
| HpyF3I | CTNAG | 1 cut(s) | 31 |
| Hsp92II | CATG | 1 cut(s) | 30 |
| LpnPI | CCDG | 2 cut(s) | 18, 112 |
| MnlI | CCTC | 2 cut(s) | 73, 139 |
| Mph1103I | ATGCAT | 1 cut(s) | 111 |
| Mva1269I | GAATGC | 1 cut(s) | 111 |
| NlaIII | CATG | 1 cut(s) | 30 |
| NsiI | ATGCAT | 1 cut(s) | 111 |
| PctI | GAATGC | 1 cut(s) | 111 |
| SetI | ASST | 3 cut(s) | 37, 84, 100 |
| SgeI | CNNG | 6 cut(s) | 39, 45, 72, 85, 111, 139 |
| SmlI | CTYRAG | 1 cut(s) | 143 |
| SmoI | CTYRAG | 1 cut(s) | 143 |
| TspDTI | ATGAA | 2 cut(s) | 15, 60 |
| Zsp2I | ATGCAT | 1 cut(s) | 111 |